hcn2 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	hcn2
Entrez Gene ID	101066607
Full Name	hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003974068.1	XP_003974117.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
XM_003974068.2	XP_003974117.2	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Pathways from BioSystems
Gene Ontology

The following hcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo58452	XM_003974068.2 Latest version!	Takifugu rubripes hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (hcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo12939	XM_003974068.1 Latest version!	Takifugu rubripes hyperpolarization activated cyclic nucleotide gated potassium channel 2 (hcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hcn2 ( XM_003974068.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003974117.2 Takifugu rubripes hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (hcn2), mRNA.

Price & Availability↑

CloneID	OTo58452
Clone ID Related Accession (Same CDS sequence)	XM_003974068.2
Accession Version	XM_003974068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042304.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003974068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAG TGGAGGAATT GAGAAAAAGC ATAAAGAAAT TTGTGCCAGG AGGCGGAGCT 
GCGTCCATTA CGCGCAAAAA GTCCTACAAC AAAGAGCCGT GCCTCCTTCG AGGGGAGGAC 
TCGGCACCTG TCAGTGACCG CGCAGGCTGC GCTCTGCTGC GCCAGAGGGA TGCTGCTGGA 
TCAGAGTCAG AGCGCAGAGG TGGAGGGGAC TCGGAGTCAG GGGCAGGCGT GGTAGGAGCC 
GCAACCATCG GAATGATATG CAACAAAAAC GGAGACTGTA GAAGGGACCC CACGAGTTCA 
GGGAGCCTCT CGTCTCTTAT ATCCCGACAA GAGAGGCGGG CGGATGGAGT GACCACACCA 
TCAGCTGAAG ACGGCACGGC TGCAGTCATG GACGGCTCCG TCACTTCTGC CGGGTCCCTC 
AGCCAGGGGC CCGGGGCCGA GGTCAAAACC ACGGCCACGT CGGAGAAAAA GGACTCCAGA 
GTTTCCTTCT CCAACGCGCC TGCCGGTAAG GGACAGACCC TGAGTCAGCA TTCAAAGAAC 
TCAAGAATCT CCTTCTCCAA AGCCGAGGAT GGGTCCCAGA TCGGGCCGGA TGACGGGGAG 
TCCAGGGGGA ACCAGACTTA CATGCAGCGG CAGTTCAGCT CCATGCTGCA GCCCGGAGTC 
AACAAATTCT CCCTGCGCAT GTTCGGTAGC CAGAAGGCGG TGGAGAAGGA GCAGGAGCGC 
GTCAAGTCAG CCGGGAACTG GATCATACAC CCGTACAGCG ACTTCAGGTT CTACTGGGAC 
TTCACCATGC TGATGTTCAT GGTGGCCAAC CTGATCATCA TCCCCGTGGG CATCACTTTC 
TTCAAGGACG AGACCACCAC GCCCTGGATC ATCTTCAACG TCATCTCCGA CACGTTCTTC 
CTCATGGACC TGGTGCTCAA TTTCAGGACC GGCATCGTGT TCGACGACAA CACCGAGATC 
ATCCTGGACC CCGACAAGAT CAAGAAGAAG TACCTGAAGA CCTGGTTCGT GGTGGACTTT 
GTCTCGTCCA TACCCGTGGA TTACATCTTC CTCATCGTGG AGAAGGGCAT CGACTCGGAG 
GTGTACAAGA CGGCCCGGGC GCTGCGCATC GTCCGCTTCA CCAAGATCCT CAGCCTGCTG 
CGTCTGCTGA GGCTGTCCAG GCTCATCCGC TACATCCACC AATGGGAGGA GATCTTCCAC 
ATGACCTATG ACCTGGCAAG TGCTGTGATG AGAATATTCA ACCTGATCGG GATGATGCTG 
CTGCTGTGCC ACTGGGACGG CTGCCTCCAG TTCCTGGTGC CCATGCTGCA GGACTTCCCA 
TCCGACTGCT GGGTTTCCCT CAACAAGATG GTGAATGATT CCTGGAGCGA GCTCTATTCC 
TTTGCCCTCT TTAAAGCCAT GAGCCACATG CTGTGTATTG GATATGGGAG ACAAGCTCCA 
GAGAGCATGT CAGACATCTG GCTCACCATG CTCAGTATGA TCGTCGGAGC CACCTGCTAC 
GCCATGTTTA TCGGCCACGC CACCGCTCTG ATCCAATCGC TGGACTCGTC TAGACGCCAG 
TACCAAGAAA AGTACAAACA AGTGGAGCAG TACATGTCCT TCCACAAACT ACCGGCAGAC 
TTCCGACAGA AAATCCATGA CTACTACGAG CACCGATACC AAGGCAAGAT GTTTGACGAA 
GAGAGCATCC TGGAGGAGCT TAACGAGCCT CTGCGGGAGG AGATCGTCAA CTTCAACTGC 
CGTAAGCTGG TAGCCTCCAT GCCGCTGTTT GCCAACGCCG ATCCCAACTT TGTGACGGCC 
ATGCTGACCA AGCTGAGGTT TGAGGTCTTC CAGCCCAAAG ATTACATCGT CAGAGAGGGC 
ACCATCGGAA AGAAGATGTT CTTCATCCAG CACGGGGTGG TGAGCATCCT GACCAAAGGG 
AACATCAGCA TGAAGCTCAT GGATGGCTCC TACTTTGGAG AGATCTGCCT GCTGACCCGC 
GGCAGACGGA CGGCGAGTGT GCAGGCGGAG ACCTACTGCC GCCTGTACTC GCTCTCTGTG 
GACAACTTCA ACGAGGTGCT GGAGGAGTAT CCCATGATGA GGAGGGCCTT CGAGACGGTC 
GCCATCGACA GGCTGGACAG GATCGGCAAG AAAAACTCCA TCCTGATGCA CAAAGTTCAA 
CACGATCTCA ACTCCGGCGT CTTCAACAAC CGCGAGAACG AGATGATCCA GGAGATCGTC 
AAATATGACC GGGAGATGGT CCAGCAGGTG GACGCTCCTC GACCGCGGGC CATGTCCCTG 
ACTCCCCCCC TGCTCGGAGG GATGTTTCCG CACTCGGGCC CGACGGCACA GCCACAGAAT 
TCAGCTATCG CCTCCCTGCA GCAGGCCGTA GCCATGAGCT TTTGCCCCCC CGTCCAGGAC 
ACCTCTGCGG GTGGGGGAGG AGGACTCCAG TCGCCCAGGA TGCTGAGGAG ACTCCAAGTG 
ACTGAAGGCA TATCCACCCC AGCCTCATCC TCCCCGCTGC AGTCCCAGCT CCTCGCTTCT 
GGTGCCTTTG CTCCCATGCT GTGCAGCCCC CCCGTCCAGA GCCCCTTAGC CTTTGGAGGA 
CGGTCTTTCC AGTATGGATC CAGTGGCCTG GCGGGTCCAT TTTCAGGCTC CCAGCTTTCT 
CTCACCCAGC AACACTTATC CAGCCCGGTC CACAGGCCCA GCACACACAA GAGTACCCAC 
TCCCTGCCGA TGGGGGGCCT GAGCCAGGAC GCCAGGGCAC TGTCAGCCTC CCAGCCCTCG 
CTGCCCCACG AGGGAGCGCA GCCGCACGGT CCGAGTCCAC CCCAGTCAAC GCGAGTCTCC 
TCCACATCCA TTGGACCCCC TCAGACCCCG TCGGGCCACG CGGGCATCCG GAGCGCGGTG 
GTTCACAGGG TGGTGCTGCC CCACCAGATG TCTGTGGGGG CCTTCCCTGT AGGCTCCCTG 
CCAGGGTCTG CACACAGCTT CGGTGCCAGT GTGGGGCTGG TGGTAGGGGG CGTGGGTACG 
GGTCTGAGAG ACCCTGGACT GCCCAAGAAG GACTCCATAT CCAGCATTCC CGAGACAGAT 
CACATCAAAG TGAGATCCAG GCTATCCTCA AACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003974117.2
CDS	872..3970
Translation	MGAVEELRKSIKKFVPGGGAASITRKKSYNKEPCLLRGEDSAPVSDRAGCALLRQRDAAGSESERRGGGDSESGAGVVGAATIGMICNKNGDCRRDPTSSGSLSSLISRQERRADGVTTPSAEDGTAAVMDGSVTSAGSLSQGPGAEVKTTATSEKKDSRVSFSNAPAGKGQTLSQHSKNSRISFSKAEDGSQIGPDDGESRGNQTYMQRQFSSMLQPGVNKFSLRMFGSQKAVEKEQERVKSAGNWIIHPYSDFRFYWDFTMLMFMVANLIIIPVGITFFKDETTTPWIIFNVISDTFFLMDLVLNFRTGIVFDDNTEIILDPDKIKKKYLKTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSDCWVSLNKMVNDSWSELYSFALFKAMSHMLCIGYGRQAPESMSDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEESILEELNEPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLRFEVFQPKDYIVREGTIGKKMFFIQHGVVSILTKGNISMKLMDGSYFGEICLLTRGRRTASVQAETYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILMHKVQHDLNSGVFNNRENEMIQEIVKYDREMVQQVDAPRPRAMSLTPPLLGGMFPHSGPTAQPQNSAIASLQQAVAMSFCPPVQDTSAGGGGGLQSPRMLRRLQVTEGISTPASSSPLQSQLLASGAFAPMLCSPPVQSPLAFGGRSFQYGSSGLAGPFSGSQLSLTQQHLSSPVHRPSTHKSTHSLPMGGLSQDARALSASQPSLPHEGAQPHGPSPPQSTRVSSTSIGPPQTPSGHAGIRSAVVHRVVLPHQMSVGAFPVGSLPGSAHSFGASVGLVVGGVGTGLRDPGLPKKDSISSIPETDHIKVRSRLSSNL

Target ORF information:

RefSeq Version	XM_003974068.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (hcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003974068.2

ORF Insert Sequence:

ATGGGAGCAG TGGAGGAATT GAGAAAAAGC ATAAAGAAAT TTGTGCCAGG AGGCGGAGCT 
GCGTCCATTA CGCGCAAAAA GTCCTACAAC AAAGAGCCGT GCCTCCTTCG AGGGGAGGAC 
TCGGCACCTG TCAGTGACCG CGCAGGCTGC GCTCTGCTGC GCCAGAGGGA TGCTGCTGGA 
TCAGAGTCAG AGCGCAGAGG TGGAGGGGAC TCGGAGTCAG GGGCAGGCGT GGTAGGAGCC 
GCAACCATCG GAATGATATG CAACAAAAAC GGAGACTGTA GAAGGGACCC CACGAGTTCA 
GGGAGCCTCT CGTCTCTTAT ATCCCGACAA GAGAGGCGGG CGGATGGAGT GACCACACCA 
TCAGCTGAAG ACGGCACGGC TGCAGTCATG GACGGCTCCG TCACTTCTGC CGGGTCCCTC 
AGCCAGGGGC CCGGGGCCGA GGTCAAAACC ACGGCCACGT CGGAGAAAAA GGACTCCAGA 
GTTTCCTTCT CCAACGCGCC TGCCGGTAAG GGACAGACCC TGAGTCAGCA TTCAAAGAAC 
TCAAGAATCT CCTTCTCCAA AGCCGAGGAT GGGTCCCAGA TCGGGCCGGA TGACGGGGAG 
TCCAGGGGGA ACCAGACTTA CATGCAGCGG CAGTTCAGCT CCATGCTGCA GCCCGGAGTC 
AACAAATTCT CCCTGCGCAT GTTCGGTAGC CAGAAGGCGG TGGAGAAGGA GCAGGAGCGC 
GTCAAGTCAG CCGGGAACTG GATCATACAC CCGTACAGCG ACTTCAGGTT CTACTGGGAC 
TTCACCATGC TGATGTTCAT GGTGGCCAAC CTGATCATCA TCCCCGTGGG CATCACTTTC 
TTCAAGGACG AGACCACCAC GCCCTGGATC ATCTTCAACG TCATCTCCGA CACGTTCTTC 
CTCATGGACC TGGTGCTCAA TTTCAGGACC GGCATCGTGT TCGACGACAA CACCGAGATC 
ATCCTGGACC CCGACAAGAT CAAGAAGAAG TACCTGAAGA CCTGGTTCGT GGTGGACTTT 
GTCTCGTCCA TACCCGTGGA TTACATCTTC CTCATCGTGG AGAAGGGCAT CGACTCGGAG 
GTGTACAAGA CGGCCCGGGC GCTGCGCATC GTCCGCTTCA CCAAGATCCT CAGCCTGCTG 
CGTCTGCTGA GGCTGTCCAG GCTCATCCGC TACATCCACC AATGGGAGGA GATCTTCCAC 
ATGACCTATG ACCTGGCAAG TGCTGTGATG AGAATATTCA ACCTGATCGG GATGATGCTG 
CTGCTGTGCC ACTGGGACGG CTGCCTCCAG TTCCTGGTGC CCATGCTGCA GGACTTCCCA 
TCCGACTGCT GGGTTTCCCT CAACAAGATG GTGAATGATT CCTGGAGCGA GCTCTATTCC 
TTTGCCCTCT TTAAAGCCAT GAGCCACATG CTGTGTATTG GATATGGGAG ACAAGCTCCA 
GAGAGCATGT CAGACATCTG GCTCACCATG CTCAGTATGA TCGTCGGAGC CACCTGCTAC 
GCCATGTTTA TCGGCCACGC CACCGCTCTG ATCCAATCGC TGGACTCGTC TAGACGCCAG 
TACCAAGAAA AGTACAAACA AGTGGAGCAG TACATGTCCT TCCACAAACT ACCGGCAGAC 
TTCCGACAGA AAATCCATGA CTACTACGAG CACCGATACC AAGGCAAGAT GTTTGACGAA 
GAGAGCATCC TGGAGGAGCT TAACGAGCCT CTGCGGGAGG AGATCGTCAA CTTCAACTGC 
CGTAAGCTGG TAGCCTCCAT GCCGCTGTTT GCCAACGCCG ATCCCAACTT TGTGACGGCC 
ATGCTGACCA AGCTGAGGTT TGAGGTCTTC CAGCCCAAAG ATTACATCGT CAGAGAGGGC 
ACCATCGGAA AGAAGATGTT CTTCATCCAG CACGGGGTGG TGAGCATCCT GACCAAAGGG 
AACATCAGCA TGAAGCTCAT GGATGGCTCC TACTTTGGAG AGATCTGCCT GCTGACCCGC 
GGCAGACGGA CGGCGAGTGT GCAGGCGGAG ACCTACTGCC GCCTGTACTC GCTCTCTGTG 
GACAACTTCA ACGAGGTGCT GGAGGAGTAT CCCATGATGA GGAGGGCCTT CGAGACGGTC 
GCCATCGACA GGCTGGACAG GATCGGCAAG AAAAACTCCA TCCTGATGCA CAAAGTTCAA 
CACGATCTCA ACTCCGGCGT CTTCAACAAC CGCGAGAACG AGATGATCCA GGAGATCGTC 
AAATATGACC GGGAGATGGT CCAGCAGGTG GACGCTCCTC GACCGCGGGC CATGTCCCTG 
ACTCCCCCCC TGCTCGGAGG GATGTTTCCG CACTCGGGCC CGACGGCACA GCCACAGAAT 
TCAGCTATCG CCTCCCTGCA GCAGGCCGTA GCCATGAGCT TTTGCCCCCC CGTCCAGGAC 
ACCTCTGCGG GTGGGGGAGG AGGACTCCAG TCGCCCAGGA TGCTGAGGAG ACTCCAAGTG 
ACTGAAGGCA TATCCACCCC AGCCTCATCC TCCCCGCTGC AGTCCCAGCT CCTCGCTTCT 
GGTGCCTTTG CTCCCATGCT GTGCAGCCCC CCCGTCCAGA GCCCCTTAGC CTTTGGAGGA 
CGGTCTTTCC AGTATGGATC CAGTGGCCTG GCGGGTCCAT TTTCAGGCTC CCAGCTTTCT 
CTCACCCAGC AACACTTATC CAGCCCGGTC CACAGGCCCA GCACACACAA GAGTACCCAC 
TCCCTGCCGA TGGGGGGCCT GAGCCAGGAC GCCAGGGCAC TGTCAGCCTC CCAGCCCTCG 
CTGCCCCACG AGGGAGCGCA GCCGCACGGT CCGAGTCCAC CCCAGTCAAC GCGAGTCTCC 
TCCACATCCA TTGGACCCCC TCAGACCCCG TCGGGCCACG CGGGCATCCG GAGCGCGGTG 
GTTCACAGGG TGGTGCTGCC CCACCAGATG TCTGTGGGGG CCTTCCCTGT AGGCTCCCTG 
CCAGGGTCTG CACACAGCTT CGGTGCCAGT GTGGGGCTGG TGGTAGGGGG CGTGGGTACG 
GGTCTGAGAG ACCCTGGACT GCCCAAGAAG GACTCCATAT CCAGCATTCC CGAGACAGAT 
CACATCAAAG TGAGATCCAG GCTATCCTCA AACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hcn2 ( XM_003974068.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003974117.1 Takifugu rubripes hyperpolarization activated cyclic nucleotide gated potassium channel 2 (hcn2), mRNA.

Price & Availability↑

CloneID	OTo12939
Clone ID Related Accession (Same CDS sequence)	XM_003974068.1
Accession Version	XM_003974068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071143.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 28% of CDS bases ##RefSeq-Attributes-END##

ATGGGAGCAG TGGAGGAATT GAGAAAAAGC ATAAAGAAAT TTGTGCCAGG AGGCGGAGCT 
GCGTCCATTA CGCGCAAAAA GTCCTACAAC AAAGAGCCGT GCCTCCTTCG AGGGGAGGAC 
TCGGCACCTG TCAGTGACCG CGCAGGCTGC GCTCTGCTGC GCCAGAGGGA TGCTGCTGGA 
TCAGAGTCAG AGCGCAGAGG TGGAGGGGAC TCGGAGTCAG GGGCAGGCGT GGTAGGAGCC 
GCAACCATCG GAATGATATG CAACAAAAAC GGAGACTGTA GAAGGGACCC CACGAGTTCA 
GGGAGCCTCT CGTCTCTTAT ATCCCGACAA GAGAGGCGGG CGGATGGAGT GACCACACCA 
TCAGCTGAAG ACGGCACGGC TGCAGTCATG GACGGCTCCG TCACTTCTGC CGGGTCCCTC 
AGCCAGGGGC CCGGGGCCGA GGTCAAAACC ACGGCCACGT CGGAGAAAAA GGACTCCAGA 
GTTTCCTTCT CCAACGCGCC TGCCGGTAAG GGACAGACCC TGAGTCAGCA TTCAAAGAAC 
TCAAGAATCT CCTTCTCCAA AGCCGAGGAT GGGTCCCAGA TCGGGCCGGA TGACGGGGAG 
TCCAGGGGGA ACCAGACTTA CATGCAGCGG CAGTTCAGCT CCATGCTGCA GCCCGGAGTC 
AACAAATTCT CCCTGCGCAT GTTCGGTAGC CAGAAGGCGG TGGAGAAGGA GCAGGAGCGC 
GTCAAGTCAG CCGGGAACTG GATCATACAC CCGTACAGCG ACTTCAGGTT CTACTGGGAC 
TTCACCATGC TGATGTTCAT GGTGGCCAAC CTGATCATCA TCCCCGTGGG CATCACTTTC 
TTCAAGGACG AGACCACCAC GCCCTGGATC ATCTTCAACG TCATCTCCGA CACGTTCTTC 
CTCATGGACC TGGTGCTCAA TTTCAGGACC GGCATCGTGT TCGACGACAA CACCGAGATC 
ATCCTGGACC CCGACAAGAT CAAGAAGAAG TACCTGAAGA CCTGGTTCGT GGTGGACTTT 
GTCTCGTCCA TACCCGTGGA TTACATCTTC CTCATCGTGG AGAAGGGCAT CGACTCGGAG 
GTGTACAAGA CGGCCCGGGC GCTGCGCATC GTCCGCTTCA CCAAGATCCT CAGCCTGCTG 
CGTCTGCTGA GGCTGTCCAG GCTCATCCGC TACATCCACC AATGGGAGGA GATCTTCCAC 
ATGACCTATG ACCTGGCAAG TGCTGTGATG AGAATATTCA ACCTGATCGG GATGATGCTG 
CTGCTGTGCC ACTGGGACGG CTGCCTCCAG TTCCTGGTGC CCATGCTGCA GGACTTCCCA 
TCCGACTGCT GGGTTTCCCT CAACAAGATG GTGAATGATT CCTGGAGCGA GCTCTATTCC 
TTTGCCCTCT TTAAAGCCAT GAGCCACATG CTGTGTATTG GATATGGGAG ACAAGCTCCA 
GAGAGCATGT CAGACATCTG GCTCACCATG CTCAGTATGA TCGTCGGAGC CACCTGCTAC 
GCCATGTTTA TCGGCCACGC CACCGCTCTG ATCCAATCGC TGGACTCGTC TAGACGCCAG 
TACCAAGAAA AGTACAAACA AGTGGAGCAG TACATGTCCT TCCACAAACT ACCGGCAGAC 
TTCCGACAGA AAATCCATGA CTACTACGAG CACCGATACC AAGGCAAGAT GTTTGACGAA 
GAGAGCATCC TGGAGGAGCT TAACGAGCCT CTGCGGGAGG AGATCGTCAA CTTCAACTGC 
CGTAAGCTGG TAGCCTCCAT GCCGCTGTTT GCCAACGCCG ATCCCAACTT TGTGACGGCC 
ATGCTGACCA AGCTGAGGTT TGAGGTCTTC CAGCCCAAAG ATTACATCGT CAGAGAGGGC 
ACCATCGGAA AGAAGATGTT CTTCATCCAG CACGGGGTGG TGAGCATCCT GACCAAAGGG 
AACATCAGCA TGAAGCTCAT GGATGGCTCC TACTTTGGAG AGATCTGCCT GCTGACCCGC 
GGCAGACGGA CGGCGAGTGT GCAGGCGGAG ACCTACTGCC GCCTGTACTC GCTCTCTGTG 
GACAACTTCA ACGAGGTGCT GGAGGAGTAT CCCATGATGA GGAGGGCCTT CGAGACGGTC 
GCCATCGACA GGCTGGACAG GATCGGCAAG AAAAACTCCA TCCTGATGCA CAAAGTTCAA 
CACGATCTCA ACTCCGGCGT CTTCAACAAC CGCGAGAACG AGATGATCCA GGAGATCGTC 
AAATATGACC GGGAGATGGT CCAGCAGGTG GACGCTCCTC GACCGCGGGC CATGTCCCTG 
ACTCCCCCCC TGCTCGGAGG GATGTTTCCG CACTCGGGCC CGACGGCACA GCCACAGAAT 
TCAGCTATCG CCTCCCTGCA GCAGGCCGTA GCCATGAGCT TTTGCCCCCC CGTCCAGGAC 
ACCTCTGCGG GTGGGGGAGG AGGACTCCAG TCGCCCAGGA TGCTGAGGAG ACTCCAAGTG 
ACTGAAGGCA TATCCACCCC AGCCTCATCC TCCCCGCTGC AGTCCCAGCT CCTCGCTTCT 
GGTGCCTTTG CTCCCATGCT GTGCAGCCCC CCCGTCCAGA GCCCCTTAGC CTTTGGAGGA 
CGGTCTTTCC AGTATGGATC CAGTGGCCTG GCGGGTCCAT TTTCAGGCTC CCAGCTTTCT 
CTCACCCAGC AACACTTATC CAGCCCGGTC CACAGGCCCA GCACACACAA GAGTACCCAC 
TCCCTGCCGA TGGGGGGCCT GAGCCAGGAC GCCAGGGCAC TGTCAGCCTC CCAGCCCTCG 
CTGCCCCACG AGGGAGCGCA GCCACACGGT CCGAGTCCAC CCCAGTCAAC GCGAGTCTCC 
TCCACATCCA TTGGACCCCC TCAGACCCCG TCGGGCCACG CGGGCATCCG GAGCGCGGTG 
GTTCACAGGG TGGTGCTGCC CCACCAGATG TCTGTGGGGG CCTTCCCTGT AGGCTCCCTG 
CCAGGGTCTG CACACAGCTT CGGTGCCAGT GTGGGGCTGG TGGTAGGGGG CGTGGGTACG 
GGTCTGAGAG ACCCTGGACT GCCCAAGAAG GACTCCATAT GCAGCATTCC CGAGACAGAT 
CACATCAAAG TGAGATCCAG GCTATCCTCA AACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003974117.1
CDS	1..3099
Translation	MGAVEELRKSIKKFVPGGGAASITRKKSYNKEPCLLRGEDSAPVSDRAGCALLRQRDAAGSESERRGGGDSESGAGVVGAATIGMICNKNGDCRRDPTSSGSLSSLISRQERRADGVTTPSAEDGTAAVMDGSVTSAGSLSQGPGAEVKTTATSEKKDSRVSFSNAPAGKGQTLSQHSKNSRISFSKAEDGSQIGPDDGESRGNQTYMQRQFSSMLQPGVNKFSLRMFGSQKAVEKEQERVKSAGNWIIHPYSDFRFYWDFTMLMFMVANLIIIPVGITFFKDETTTPWIIFNVISDTFFLMDLVLNFRTGIVFDDNTEIILDPDKIKKKYLKTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSDCWVSLNKMVNDSWSELYSFALFKAMSHMLCIGYGRQAPESMSDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEESILEELNEPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLRFEVFQPKDYIVREGTIGKKMFFIQHGVVSILTKGNISMKLMDGSYFGEICLLTRGRRTASVQAETYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILMHKVQHDLNSGVFNNRENEMIQEIVKYDREMVQQVDAPRPRAMSLTPPLLGGMFPHSGPTAQPQNSAIASLQQAVAMSFCPPVQDTSAGGGGGLQSPRMLRRLQVTEGISTPASSSPLQSQLLASGAFAPMLCSPPVQSPLAFGGRSFQYGSSGLAGPFSGSQLSLTQQHLSSPVHRPSTHKSTHSLPMGGLSQDARALSASQPSLPHEGAQPHGPSPPQSTRVSSTSIGPPQTPSGHAGIRSAVVHRVVLPHQMSVGAFPVGSLPGSAHSFGASVGLVVGGVGTGLRDPGLPKKDSICSIPETDHIKVRSRLSSNL

Target ORF information:

RefSeq Version	XM_003974068.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes hyperpolarization activated cyclic nucleotide gated potassium channel 2 (hcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003974068.1

ORF Insert Sequence:

ATGGGAGCAG TGGAGGAATT GAGAAAAAGC ATAAAGAAAT TTGTGCCAGG AGGCGGAGCT 
GCGTCCATTA CGCGCAAAAA GTCCTACAAC AAAGAGCCGT GCCTCCTTCG AGGGGAGGAC 
TCGGCACCTG TCAGTGACCG CGCAGGCTGC GCTCTGCTGC GCCAGAGGGA TGCTGCTGGA 
TCAGAGTCAG AGCGCAGAGG TGGAGGGGAC TCGGAGTCAG GGGCAGGCGT GGTAGGAGCC 
GCAACCATCG GAATGATATG CAACAAAAAC GGAGACTGTA GAAGGGACCC CACGAGTTCA 
GGGAGCCTCT CGTCTCTTAT ATCCCGACAA GAGAGGCGGG CGGATGGAGT GACCACACCA 
TCAGCTGAAG ACGGCACGGC TGCAGTCATG GACGGCTCCG TCACTTCTGC CGGGTCCCTC 
AGCCAGGGGC CCGGGGCCGA GGTCAAAACC ACGGCCACGT CGGAGAAAAA GGACTCCAGA 
GTTTCCTTCT CCAACGCGCC TGCCGGTAAG GGACAGACCC TGAGTCAGCA TTCAAAGAAC 
TCAAGAATCT CCTTCTCCAA AGCCGAGGAT GGGTCCCAGA TCGGGCCGGA TGACGGGGAG 
TCCAGGGGGA ACCAGACTTA CATGCAGCGG CAGTTCAGCT CCATGCTGCA GCCCGGAGTC 
AACAAATTCT CCCTGCGCAT GTTCGGTAGC CAGAAGGCGG TGGAGAAGGA GCAGGAGCGC 
GTCAAGTCAG CCGGGAACTG GATCATACAC CCGTACAGCG ACTTCAGGTT CTACTGGGAC 
TTCACCATGC TGATGTTCAT GGTGGCCAAC CTGATCATCA TCCCCGTGGG CATCACTTTC 
TTCAAGGACG AGACCACCAC GCCCTGGATC ATCTTCAACG TCATCTCCGA CACGTTCTTC 
CTCATGGACC TGGTGCTCAA TTTCAGGACC GGCATCGTGT TCGACGACAA CACCGAGATC 
ATCCTGGACC CCGACAAGAT CAAGAAGAAG TACCTGAAGA CCTGGTTCGT GGTGGACTTT 
GTCTCGTCCA TACCCGTGGA TTACATCTTC CTCATCGTGG AGAAGGGCAT CGACTCGGAG 
GTGTACAAGA CGGCCCGGGC GCTGCGCATC GTCCGCTTCA CCAAGATCCT CAGCCTGCTG 
CGTCTGCTGA GGCTGTCCAG GCTCATCCGC TACATCCACC AATGGGAGGA GATCTTCCAC 
ATGACCTATG ACCTGGCAAG TGCTGTGATG AGAATATTCA ACCTGATCGG GATGATGCTG 
CTGCTGTGCC ACTGGGACGG CTGCCTCCAG TTCCTGGTGC CCATGCTGCA GGACTTCCCA 
TCCGACTGCT GGGTTTCCCT CAACAAGATG GTGAATGATT CCTGGAGCGA GCTCTATTCC 
TTTGCCCTCT TTAAAGCCAT GAGCCACATG CTGTGTATTG GATATGGGAG ACAAGCTCCA 
GAGAGCATGT CAGACATCTG GCTCACCATG CTCAGTATGA TCGTCGGAGC CACCTGCTAC 
GCCATGTTTA TCGGCCACGC CACCGCTCTG ATCCAATCGC TGGACTCGTC TAGACGCCAG 
TACCAAGAAA AGTACAAACA AGTGGAGCAG TACATGTCCT TCCACAAACT ACCGGCAGAC 
TTCCGACAGA AAATCCATGA CTACTACGAG CACCGATACC AAGGCAAGAT GTTTGACGAA 
GAGAGCATCC TGGAGGAGCT TAACGAGCCT CTGCGGGAGG AGATCGTCAA CTTCAACTGC 
CGTAAGCTGG TAGCCTCCAT GCCGCTGTTT GCCAACGCCG ATCCCAACTT TGTGACGGCC 
ATGCTGACCA AGCTGAGGTT TGAGGTCTTC CAGCCCAAAG ATTACATCGT CAGAGAGGGC 
ACCATCGGAA AGAAGATGTT CTTCATCCAG CACGGGGTGG TGAGCATCCT GACCAAAGGG 
AACATCAGCA TGAAGCTCAT GGATGGCTCC TACTTTGGAG AGATCTGCCT GCTGACCCGC 
GGCAGACGGA CGGCGAGTGT GCAGGCGGAG ACCTACTGCC GCCTGTACTC GCTCTCTGTG 
GACAACTTCA ACGAGGTGCT GGAGGAGTAT CCCATGATGA GGAGGGCCTT CGAGACGGTC 
GCCATCGACA GGCTGGACAG GATCGGCAAG AAAAACTCCA TCCTGATGCA CAAAGTTCAA 
CACGATCTCA ACTCCGGCGT CTTCAACAAC CGCGAGAACG AGATGATCCA GGAGATCGTC 
AAATATGACC GGGAGATGGT CCAGCAGGTG GACGCTCCTC GACCGCGGGC CATGTCCCTG 
ACTCCCCCCC TGCTCGGAGG GATGTTTCCG CACTCGGGCC CGACGGCACA GCCACAGAAT 
TCAGCTATCG CCTCCCTGCA GCAGGCCGTA GCCATGAGCT TTTGCCCCCC CGTCCAGGAC 
ACCTCTGCGG GTGGGGGAGG AGGACTCCAG TCGCCCAGGA TGCTGAGGAG ACTCCAAGTG 
ACTGAAGGCA TATCCACCCC AGCCTCATCC TCCCCGCTGC AGTCCCAGCT CCTCGCTTCT 
GGTGCCTTTG CTCCCATGCT GTGCAGCCCC CCCGTCCAGA GCCCCTTAGC CTTTGGAGGA 
CGGTCTTTCC AGTATGGATC CAGTGGCCTG GCGGGTCCAT TTTCAGGCTC CCAGCTTTCT 
CTCACCCAGC AACACTTATC CAGCCCGGTC CACAGGCCCA GCACACACAA GAGTACCCAC 
TCCCTGCCGA TGGGGGGCCT GAGCCAGGAC GCCAGGGCAC TGTCAGCCTC CCAGCCCTCG 
CTGCCCCACG AGGGAGCGCA GCCACACGGT CCGAGTCCAC CCCAGTCAAC GCGAGTCTCC 
TCCACATCCA TTGGACCCCC TCAGACCCCG TCGGGCCACG CGGGCATCCG GAGCGCGGTG 
GTTCACAGGG TGGTGCTGCC CCACCAGATG TCTGTGGGGG CCTTCCCTGT AGGCTCCCTG 
CCAGGGTCTG CACACAGCTT CGGTGCCAGT GTGGGGCTGG TGGTAGGGGG CGTGGGTACG 
GGTCTGAGAG ACCCTGGACT GCCCAAGAAG GACTCCATAT GCAGCATTCC CGAGACAGAT 
CACATCAAAG TGAGATCCAG GCTATCCTCA AACTTGTGA