EPO cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPO
Entrez Gene ID	397249
Full Name	erythropoietin
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 3 Map Location: 3\|3

mRNA and Protein(s)

mRNA	Protein	Name
NM_214134.1	NP_999299.1	erythropoietin precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Localization of erythropoietin in and around growing cartilage.
De Spiegelaere W, Cornillie P, Van den Broeck W
Molecular and cellular biochemistry337(1-2)287-91(2010 Apr)

Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass.
Ryou MG, Flaherty DC, Hoxha B, Sun J, Gurji H, Rodriguez S, Bell G, Olivencia-Yurvati AH, Mallet RT
American journal of physiology. Heart and circulatory physiology297(5)H1914-22(2009 Nov)

The porcine erythropoietin gene: cDNA sequence, genomic sequence and expression analyses in piglets.
David RB, Blom AK, Sjaastad OV, Harbitz I
Domestic animal endocrinology20(2)137-47(2001 Feb)

Erythropoietin structure-function relationships: high degree of sequence homology among mammals.
Wen D, Boissel JP, Tracy TE, Gruninger RH, Mulcahy LS, Czelusniak J, Goodman M, Bunn HF
Blood82(5)1507-16(1993 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EPO might play a role as a survival factor or as a mitogen in developing cartilage tissue.
Title: Localization of erythropoietin in and around growing cartilage.

Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass.
Title: Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass.

The following EPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00158	NM_214134.1 Latest version!	Sus scrofa erythropoietin (EPO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPO ( NM_214134.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999299.1 Sus scrofa erythropoietin (EPO), mRNA.

Price & Availability↑

CloneID	OSe00158
Clone ID Related Accession (Same CDS sequence)	NM_214134.1
Accession Version	NM_214134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Sus scrofa(pig)
Product	erythropoietin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ249745.1. ##Evidence-Data-START## Transcript exon combination :: AJ249745.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGGGCGC GCGAATGTCC TGCCCGGCTG CTTCTGCTGT CCTTGCTGCT GCTTCCTCTG 
GGCCTCCCAG TCCTGGGCGC CCCCCCACGC CTCATCTGTG ACAGCCGAGT CCTGGAGAGG 
TACATCTTGG AGGCCAAGGA GGGCGAAAAT GCCACGATGG GCTGTGCCGA AAGCTGCAGC 
TTCAGTGAGA ATATCACTGT CCCAGACACC AAGGTTAACT TCTATGCCTG GAAGAGGATG 
GAGGTCCAGC AGCAGGCCAT GGAGGTCTGG CAGGGTCTGG CCCTGCTCTC AGAAGCCATC 
CTGCAGGGCC AGGCCCTGTT GGCCAACTCC TCCCAGCCAT CTGAGGCCCT GCAGCTGCAT 
GTGGACAAAG CTGTCAGCGG CCTGCGCAGC CTCACCTCCC TGCTTCGGGC GCTGGGAGCC 
CAGAAGGAAG CCATCCCCCT TCCAGACGCA TCCCCCTCCT CTGCCACCCC ACTCCGAACA 
TTTGCTGTTG ATACTTTGTG CAAACTTTTC CGCAACTACT CCAATTTCCT GCGGGGAAAG 
CTGACGCTGT ACACTGGAGA GGCCTGCAGG AGAAGGGACA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999299.1
CDS	120..704
Translation	MGARECPARLLLLSLLLLPLGLPVLGAPPRLICDSRVLERYILEAKEGENATMGCAESCSFSENITVPDTKVNFYAWKRMEVQQQAMEVWQGLALLSEAILQGQALLANSSQPSEALQLHVDKAVSGLRSLTSLLRALGAQKEAIPLPDASPSSATPLRTFAVDTLCKLFRNYSNFLRGKLTLYTGEACRRRDR

Target ORF information:

RefSeq Version	NM_214134.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa erythropoietin (EPO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214134.1

ORF Insert Sequence:

ATGGGGGCGC GCGAATGTCC TGCCCGGCTG CTTCTGCTGT CCTTGCTGCT GCTTCCTCTG 
GGCCTCCCAG TCCTGGGCGC CCCCCCACGC CTCATCTGTG ACAGCCGAGT CCTGGAGAGG 
TACATCTTGG AGGCCAAGGA GGGCGAAAAT GCCACGATGG GCTGTGCCGA AAGCTGCAGC 
TTCAGTGAGA ATATCACTGT CCCAGACACC AAGGTTAACT TCTATGCCTG GAAGAGGATG 
GAGGTCCAGC AGCAGGCCAT GGAGGTCTGG CAGGGTCTGG CCCTGCTCTC AGAAGCCATC 
CTGCAGGGCC AGGCCCTGTT GGCCAACTCC TCCCAGCCAT CTGAGGCCCT GCAGCTGCAT 
GTGGACAAAG CTGTCAGCGG CCTGCGCAGC CTCACCTCCC TGCTTCGGGC GCTGGGAGCC 
CAGAAGGAAG CCATCCCCCT TCCAGACGCA TCCCCCTCCT CTGCCACCCC ACTCCGAACA 
TTTGCTGTTG ATACTTTGTG CAAACTTTTC CGCAACTACT CCAATTTCCT GCGGGGAAAG 
CTGACGCTGT ACACTGGAGA GGCCTGCAGG AGAAGGGACA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Localization of erythropoietin in and around growing cartilage.
Molecular and cellular biochemistry337(1-2)287-91(2010 Apr)
De Spiegelaere W,Cornillie P,Van den Broeck W

Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass.
American journal of physiology. Heart and circulatory physiology297(5)H1914-22(2009 Nov)
Ryou MG,Flaherty DC,Hoxha B,Sun J,Gurji H,Rodriguez S,Bell G,Olivencia-Yurvati AH,Mallet RT

The porcine erythropoietin gene: cDNA sequence, genomic sequence and expression analyses in piglets.
Domestic animal endocrinology20(2)137-47(2001 Feb)
David RB,Blom AK,Sjaastad OV,Harbitz I

Erythropoietin structure-function relationships: high degree of sequence homology among mammals.
Blood82(5)1507-16(1993 Sep)
Wen D,Boissel JP,Tracy TE,Gruninger RH,Mulcahy LS,Czelusniak J,Goodman M,Bunn HF