ACY1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACY1
Entrez Gene ID	396930
Full Name	aminoacylase 1
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 13 Map Location: 13\|13

mRNA and Protein(s)

mRNA	Protein	Name
NM_213896.1	NP_999061.1	aminoacylase-1
XM_021068527.1	XP_020924186.1	aminoacylase-1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Probing the acyl-binding pocket of aminoacylase-1.
Lindner HA, Alary A, Wilke M, Sulea T
Biochemistry47(14)4266-75(2008 Apr)

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

Probing the catalytic center of porcine aminoacylase 1 by site-directed mutagenesis, homology modeling and substrate docking.
Liu Z, Zhen Z, Zuo Z, Wu Y, Liu A, Yi Q, Li W
Journal of biochemistry139(3)421-30(2006 Mar)

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
J?rgensen FG, Hobolth A, Hornsh?j H, Bendixen C, Fredholm M, Schierup MH
BMC biology32(2005 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

T347S variant of human Acy1 exhibited markedly increased catalytic efficiency against N-benzoylamino acids
Title: Probing the acyl-binding pocket of aminoacylase-1.

Based on site-directed mutagenesis, homology modeling and substrate docking studies, a revised catalytic mechanism for porcine metalloenzyme aminoacylase 1 has been proposed.
Title: Probing the catalytic center of porcine aminoacylase 1 by site-directed mutagenesis, homology modeling and substrate docking.

Apo-aminoacylase followed similar rules as Holo-enzyme, which suggested the zinc ion may play more important roles on activity other than structure
Title: Aspartate-induced aminoacylase folding and forming of molten globule.

The following ACY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00252	NM_213896.1 Latest version!	Sus scrofa aminoacylase 1 (ACY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OSe234914	XM_021068527.1 Latest version!	Sus scrofa aminoacylase 1 (ACY1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACY1 ( NM_213896.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999061.1 Sus scrofa aminoacylase 1 (ACY1), mRNA.

Price & Availability↑

CloneID	OSe00252
Clone ID Related Accession (Same CDS sequence)	NM_213896.1
Accession Version	NM_213896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Sus scrofa(pig)
Product	aminoacylase-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D13514.1. ##Evidence-Data-START## Transcript exon combination :: D13514.1, AK347648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAGCA AGGGTCGCGA GGGCGAGCAC CCATCCGTGA CGCTCTTCCG TCAGTACCTG 
CGCATCCGCA CCGTCCAGCC TGAGCCCGAC TACGGGGCTG CTGTGGCCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGCCT GGGCTGTCAG AAAGTGGAGG TGGTACCTGG CCATGTGGTG 
ACCGTGCTGA CCTGGCCGGG CACCAACCCC ACACTCTCCT CCATCTTGCT CAACTCCCAC 
ACAGATGTGG TGCCTGTCTT CAAGGAGCAT TGGAGTCATG ACCCCTTTGA GGGCTTCAAG 
GATGCAGATG GCTACATCTA TGGCAGGGGC GCCCAGGACA TGAAGTGCGT CAGCATCCAG 
TACCTGGAGG CTGTGAGGAG GCTGAAGGTT GAGGGCCACC ATTTCCCCAG AACCATCCAC 
ATGACCTTTG TGCCAGATGA GGAGGTTGGA GGTCACCAAG GCATGGAGCT CTTTGTGAAG 
CGGCCCGAGT TCCAGGCCCT GAGGGCTGGC TTTGCCCTGG ATGAAGGCCT AGCCAGCCCC 
ACTGACGCCT TCACTGTCTT TTACAGTGAG CGGAGCCCCT GGTGGCTAAG GGTCACGAGC 
ACTGGGAAGC CAGGGCATGG CTCGCGCTTC ATTGAGGACA CAGCAGCAGA GAAGCTGCAC 
AAGGTCATCA ACTCTATCCT GGCTTTTCGG GAGAAGGAGA AGCAGAGGCT GCAGTCAAAC 
CAGCTGAAGC CGGGGGCTGT GACCTCCGTG AACCTGACTA TGCTAGAGGG TGGCGTGGCC 
TATAACGTCG TTCCTGCCAC CATGAGTGCC TGCTTTGACT TCCGCGTAGC ACCGGATGTG 
GACCTGAAGG CTTTCGAGGA GCAGCTGCAG AGTTGGTGCC AGGCAGCTGG CGAGGGGGTC 
ACCTTTGAGT TTGTTCAGAA GTGGATGGAG ACGCAAGTGA CATCTACTGA TGACTCAGAC 
CCCTGGTGGG CAGCATTTAG TGGGGTCTTC AAGGATATGA AGCTTGCCCT AGAGCTAGAG 
ATCTGCCCTG CTTCCACTGA CGCCCGCTAT ATTCGTGCGG CGGGGGTTCC GGCTCTGGGC 
TTCTCACCCA TGAACCACAC ACCGGTGCTG CTCCATGACC ATGATGAGCG GCTGCATGAG 
GCCGTGTTTC TCCGTGGGGT TGACATATAC ACTCAGCTGC TGTCTGCCTT GGCCAGCGTG 
CCCGCACTGC CCAGTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999061.1
CDS	58..1281
Translation	MASKGREGEHPSVTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPGHVVTVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRLKVEGHHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFYSERSPWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVTSVNLTMLEGGVAYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTFEFVQKWMETQVTSTDDSDPWWAAFSGVFKDMKLALELEICPASTDARYIRAAGVPALGFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALASVPALPSES

Target ORF information:

RefSeq Version	NM_213896.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa aminoacylase 1 (ACY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213896.1

ORF Insert Sequence:

ATGGCCAGCA AGGGTCGCGA GGGCGAGCAC CCATCCGTGA CGCTCTTCCG TCAGTACCTG 
CGCATCCGCA CCGTCCAGCC TGAGCCCGAC TACGGGGCTG CTGTGGCCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGCCT GGGCTGTCAG AAAGTGGAGG TGGTACCTGG CCATGTGGTG 
ACCGTGCTGA CCTGGCCGGG CACCAACCCC ACACTCTCCT CCATCTTGCT CAACTCCCAC 
ACAGATGTGG TGCCTGTCTT CAAGGAGCAT TGGAGTCATG ACCCCTTTGA GGGCTTCAAG 
GATGCAGATG GCTACATCTA TGGCAGGGGC GCCCAGGACA TGAAGTGCGT CAGCATCCAG 
TACCTGGAGG CTGTGAGGAG GCTGAAGGTT GAGGGCCACC ATTTCCCCAG AACCATCCAC 
ATGACCTTTG TGCCAGATGA GGAGGTTGGA GGTCACCAAG GCATGGAGCT CTTTGTGAAG 
CGGCCCGAGT TCCAGGCCCT GAGGGCTGGC TTTGCCCTGG ATGAAGGCCT AGCCAGCCCC 
ACTGACGCCT TCACTGTCTT TTACAGTGAG CGGAGCCCCT GGTGGCTAAG GGTCACGAGC 
ACTGGGAAGC CAGGGCATGG CTCGCGCTTC ATTGAGGACA CAGCAGCAGA GAAGCTGCAC 
AAGGTCATCA ACTCTATCCT GGCTTTTCGG GAGAAGGAGA AGCAGAGGCT GCAGTCAAAC 
CAGCTGAAGC CGGGGGCTGT GACCTCCGTG AACCTGACTA TGCTAGAGGG TGGCGTGGCC 
TATAACGTCG TTCCTGCCAC CATGAGTGCC TGCTTTGACT TCCGCGTAGC ACCGGATGTG 
GACCTGAAGG CTTTCGAGGA GCAGCTGCAG AGTTGGTGCC AGGCAGCTGG CGAGGGGGTC 
ACCTTTGAGT TTGTTCAGAA GTGGATGGAG ACGCAAGTGA CATCTACTGA TGACTCAGAC 
CCCTGGTGGG CAGCATTTAG TGGGGTCTTC AAGGATATGA AGCTTGCCCT AGAGCTAGAG 
ATCTGCCCTG CTTCCACTGA CGCCCGCTAT ATTCGTGCGG CGGGGGTTCC GGCTCTGGGC 
TTCTCACCCA TGAACCACAC ACCGGTGCTG CTCCATGACC ATGATGAGCG GCTGCATGAG 
GCCGTGTTTC TCCGTGGGGT TGACATATAC ACTCAGCTGC TGTCTGCCTT GGCCAGCGTG 
CCCGCACTGC CCAGTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACY1 ( XM_021068527.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020924186.1 Sus scrofa aminoacylase 1 (ACY1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe234914
Clone ID Related Accession (Same CDS sequence)	XM_021068527.1
Accession Version	XM_021068527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	aminoacylase-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCA AGGGTCGCGA GGGCGAGCAC CCATCCGTGA CGCTCTTCCG TCAGTACCTG 
CGCATCCGCA CCGTCCAGCC TGAGCCCGAC TACGGGGCTG CTGTGGCCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGCCT GGGCTGTCAG AAAGTGGAGG TGAGCCTGCG GCCCTGCGTG 
GAGAGCGGAG TCGGGCCCTG GGCAGCTCCT CACCCTGCAC TGACCACCTC TCCTCCTGGC 
CCTCTCCAGG TGGTACCTGG CCATGTGGTG ACCGTGCTGA CCTGGCCGGG CACCAACCCC 
ACACTCTCCT CCATCTTGCT CAACTCCCAC ACAGATGTGG TGCCTGTCTT CAAGGAGCAT 
TGGAGTCATG ACCCCTTTGA GGGCTTCAAG GATGCAGATG GCTACATCTA TGGCAGGGGC 
GCCCAGGACA TGAAGTGCGT CAGCATCCAG TACCTGGAGG CTGTGAGGAG GCTGAAGGTT 
GAGGGCCACC ATTTCCCCAG AACCATCCAC ATGACCTTTG TGCCAGATGA GGAGGTTGGA 
GGTCACCAAG GCATGGAGCT CTTTGTGAAG CGGCCCGAGT TCCAGGCCCT GAGGGCTGGC 
TTTGCCCTGG ATGAAGGCCT AGCCAGCCCC ACTGACGCCT TCACTGTCTT TTACAGTGAG 
CGGAGCCCCT GGTGGCTAAG GGTCACGAGC ACTGGGAAGC CAGGGCATGG CTCGCGCTTC 
ATTGAGGACA CAGCAGCAGA GAAGCTGCAC AAGGTCATCA ACTCTATCCT GGCTTTTCGG 
GAGAAGGAGA AGCAGAGGCT GCAGTCAAAC CAGCTGAAGC CGGGGGCTGT GACCTCCGTG 
AACCTGACTA TGCTAGAGGG TGGCGTGGCC TATAACGTCG TTCCTGCCAC CATGAGTGCC 
TGCTTTGACT TCCGCGTAGC ACCGGATGTG GACCTGAAGG CTTTCGAGGA GCAGCTGCAG 
AGTTGGTGCC AGGCAGCTGG CGAGGGGGTC ACCTTTGAGT TTGTTCAGAA GTGGATGGAG 
ACGCAAGTGA CATCTACTGA TGACTCAGAC CCCTGGTGGG CAGCATTTAG TGGGGTCTTC 
AAGGATATGA AGCTTGCCCT AGAGCTAGAG ATCTGCCCTG CTTCCACTGA CGCCCGCTAT 
ATTCGTGCGG CGGGGGTTCC GGCTCTGGGC TTCTCACCCA TGAACCACAC ACCGGTGCTG 
CTCCATGACC ATGATGAGCG GCTGCATGAG GCCGTGTTTC TCCGTGGGGT TGACATATAC 
ACTCAGCTGC TGTCTGCCTT GGCCAGCGTG CCCGCACTGC CCAGTGAAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020924186.1
CDS	133..1446
Translation	MASKGREGEHPSVTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVSLRPCVESGVGPWAAPHPALTTSPPGPLQVVPGHVVTVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRLKVEGHHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFYSERSPWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVTSVNLTMLEGGVAYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTFEFVQKWMETQVTSTDDSDPWWAAFSGVFKDMKLALELEICPASTDARYIRAAGVPALGFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALASVPALPSES

Target ORF information:

RefSeq Version	XM_021068527.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa aminoacylase 1 (ACY1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021068527.1

ORF Insert Sequence:

ATGGCCAGCA AGGGTCGCGA GGGCGAGCAC CCATCCGTGA CGCTCTTCCG TCAGTACCTG 
CGCATCCGCA CCGTCCAGCC TGAGCCCGAC TACGGGGCTG CTGTGGCCTT CCTTGAGGAG 
AGAGCCCGCC AGCTGGGCCT GGGCTGTCAG AAAGTGGAGG TGAGCCTGCG GCCCTGCGTG 
GAGAGCGGAG TCGGGCCCTG GGCAGCTCCT CACCCTGCAC TGACCACCTC TCCTCCTGGC 
CCTCTCCAGG TGGTACCTGG CCATGTGGTG ACCGTGCTGA CCTGGCCGGG CACCAACCCC 
ACACTCTCCT CCATCTTGCT CAACTCCCAC ACAGATGTGG TGCCTGTCTT CAAGGAGCAT 
TGGAGTCATG ACCCCTTTGA GGGCTTCAAG GATGCAGATG GCTACATCTA TGGCAGGGGC 
GCCCAGGACA TGAAGTGCGT CAGCATCCAG TACCTGGAGG CTGTGAGGAG GCTGAAGGTT 
GAGGGCCACC ATTTCCCCAG AACCATCCAC ATGACCTTTG TGCCAGATGA GGAGGTTGGA 
GGTCACCAAG GCATGGAGCT CTTTGTGAAG CGGCCCGAGT TCCAGGCCCT GAGGGCTGGC 
TTTGCCCTGG ATGAAGGCCT AGCCAGCCCC ACTGACGCCT TCACTGTCTT TTACAGTGAG 
CGGAGCCCCT GGTGGCTAAG GGTCACGAGC ACTGGGAAGC CAGGGCATGG CTCGCGCTTC 
ATTGAGGACA CAGCAGCAGA GAAGCTGCAC AAGGTCATCA ACTCTATCCT GGCTTTTCGG 
GAGAAGGAGA AGCAGAGGCT GCAGTCAAAC CAGCTGAAGC CGGGGGCTGT GACCTCCGTG 
AACCTGACTA TGCTAGAGGG TGGCGTGGCC TATAACGTCG TTCCTGCCAC CATGAGTGCC 
TGCTTTGACT TCCGCGTAGC ACCGGATGTG GACCTGAAGG CTTTCGAGGA GCAGCTGCAG 
AGTTGGTGCC AGGCAGCTGG CGAGGGGGTC ACCTTTGAGT TTGTTCAGAA GTGGATGGAG 
ACGCAAGTGA CATCTACTGA TGACTCAGAC CCCTGGTGGG CAGCATTTAG TGGGGTCTTC 
AAGGATATGA AGCTTGCCCT AGAGCTAGAG ATCTGCCCTG CTTCCACTGA CGCCCGCTAT 
ATTCGTGCGG CGGGGGTTCC GGCTCTGGGC TTCTCACCCA TGAACCACAC ACCGGTGCTG 
CTCCATGACC ATGATGAGCG GCTGCATGAG GCCGTGTTTC TCCGTGGGGT TGACATATAC 
ACTCAGCTGC TGTCTGCCTT GGCCAGCGTG CCCGCACTGC CCAGTGAAAG CTGA