FZD6 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	FZD6
Entrez Gene ID	100157360
Full Name	frizzled class receptor 6
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 4 Map Location: 4\|4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001315750.1	NP_001302679.1	frizzled-6 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa).
Zou L, Wang X, Jiang L, Wang S, Xiong X, Yang H, Gao W, Gong M, Hu CA, Yin Y
PloS one12(6)e0179421(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that knockdown of frizzled 6 (FZD6) with small interfering RNAs (siRNA) significantly reduced the cell viability of the intestinal porcine enterocyte cells (IPEC-J2).
Title: Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa).

The following FZD6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FZD6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe08206	NM_001315750.1 Latest version!	Sus scrofa frizzled class receptor 6 (FZD6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FZD6 ( NM_001315750.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001302679.1 Sus scrofa frizzled class receptor 6 (FZD6), mRNA.

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CloneID	OSe08206
Clone ID Related Accession (Same CDS sequence)	NM_001315750.1
Accession Version	NM_001315750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Sus scrofa(pig)
Product	frizzled-6 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KJ808827.1. On Oct 9, 2015 this sequence version replaced XM_001928261.4. ##Evidence-Data-START## Transcript exon combination :: KJ808827.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1487420, SAMEA1876824 [ECO:0000350] ##Evidence-Data-END##

ATGGAAATGT TTACATTTTT GTGGACATGT ATTTTTCTGC CCCTCATAAG AGGGCACAGT 
CTTTTCACCT GTGAACCAAT TACTGTTCCC AGATGTATGA AAATGGCCTA CAACATGACA 
TTTTTCCCTA ATTTGATGGG TCATTATGAC CAGAGTATTG CTGCTGTGGA AATGGAGCCT 
TTTCTCCCTC TTGCGAATCT GGAATGTTCA CCAAATGTTG AAACTTTCCT TTGCAAAGCT 
TTTGTACCAA CCTGCACAGA GCAAATTGAT GTGGTTCCAC CCTGTCGTAA ATTTTGTGAG 
AAAGTATATT CTGATTGCAA AAAATTAATG GATACTTTTG GGATCCGATG GCCTGAGGAA 
CTTGAATGTA ACAGGTTACA ATACTGTGAC GAACCTGTTC CTGCAACTTT TGATCCACAC 
ACAGATTTTC TTAGTCCTCA GAAGAAAATA GAACCAGTCC AAAGAGACAT TGGATTTTGG 
TGTCCAAGGC ATCTTAAGAC TTCTGGGGGA CAAGGCTATA AGTTTCTTGG AATTGACCAG 
TGTGCACCTC CATGCCCCAA CATGTATTTT AAGAATGATG AGTTGGAGTT TGCAAAAAGT 
TTTATTGGAA TAGTTTCAAT ATTTTGTCTT TGTGCAACTC TGTTCACATT CCTTACTTTT 
TTAATTGATG TTAGAAGATT CAGATACCCA GAGAGACCAA TTATATATTA CTCTGTCTGT 
TATAGCATTG TATCTCTTAT GTACTTTATT GGATTCTTGC TGGGCAATAG TACAGCCTGC 
AATAAGGCAG ACGAGAAGCT AGAACTTGGT GACACAGTTG TCCTAGGCTC TCAAAATAAG 
GCTTGCACCG TTTTGTTTAT GTTTTTGTAT TTTTTCACAA TGGCTGGCAC TGTGTGGTGG 
GTGATTCTTA CCATTACTTG GTTCTTAGCT GCTGGGAGAA AGTGGAGTTG TGAAGCCATT 
GAGCAAAAAG CAGTGTGGTT TCATGCCGTG GCCTGGGGAA TACCAGGTTT TCTGACCGTT 
ATGCTTCTTG CTATGAACAA AGTGGAAGGA GACAATATTA GTGGAGTTTG CTTTGTCGGT 
CTTTATGACC TGGATGCTTC TCGCTACTTT GTACTCTTGC CGCTGTGCTT TTGTGTGTTT 
GTTGGGCTGT CTCTTCTTTT AGCTGGCATT ATTTCCTTAA ATCATGTTCG ACAAGTTATA 
CAGCATGATG GCCGGAACCA AGAGAAGCTA AAGAAATTTA TGATTCGAAT CGGAGTCTTC 
AGTGGCCTGT ATCTTGTGCC ATTAGTGATG CTTCTTGGAT GTTATGTCTA TGAGCAAGTG 
AACAGGATCA CCTGGGAGAT AACTTGGGTC TCTGACCATT GTCGTCAGTA CCATATACCA 
TGTCCTTATC AGGCAAAAAC AGAAACTCGA CCAGAATTGG CATTATTTAT GATGAAATAT 
TTGATGACTT TAATTGTTGG CATCTCTGCT GTCTTCTGGG TCGGAAGCAA AAAGACATGC 
ACAGAATGGG CTGGGTTTTT TAAACGAAAT CGCAAGAAAG ATCCAATCAG TGAAAGCCGA 
AGAGTACTAC AAGAGTCATG TGAGTTTTTC TTAAAGCACA ATTCTAAAGT TAAACACAAA 
AAGAAGCACT ACAAACCAAG TTCACACAAG CTGAAGGTCA TTTCCAAATC CATGGGAACC 
AGCACAGGAG CGACAGCAAA TCATGGCACT TCTGCAGTAG CAATCACTAA CCATGACTAC 
TTAGGACAAG AAACTTTGAC AGAAATACAA ACCTCGCCAG AAACATCAGT GAGGGAAGTG 
AGAGCGGACA GAGCTAGCCC GCCCAAAGCG AGAGAACAGG ACGGTGAGGA CCCTGCCTCC 
TCAGCAGCAT CCAGCTCCAG ACTCTGCGGG GAACAGACCG ACAGGAAGGG CCGGGCAGGC 
AATGTGAAGG ATAAGAGCCG AGTGCCGGCA AGTGCGCGGA GTGAAGGAAG GGTAACTCCA 
AAGAACGATG GCACTGAAAC CAGCCCGGCA CCGAGCAACA GCCTGCAGGC CCCCAGTTCT 
TCAGAGCCAG GCAGCCTCAA AGGCTCCACA TCACTGCTTG TTCACTCGGC TTCAGGAGTT 
AGAAAAGAGC AGGGCACTGG CAGTCACTCG GATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001302679.1
CDS	268..2406
Translation	MEMFTFLWTCIFLPLIRGHSLFTCEPITVPRCMKMAYNMTFFPNLMGHYDQSIAAVEMEPFLPLANLECSPNVETFLCKAFVPTCTEQIDVVPPCRKFCEKVYSDCKKLMDTFGIRWPEELECNRLQYCDEPVPATFDPHTDFLSPQKKIEPVQRDIGFWCPRHLKTSGGQGYKFLGIDQCAPPCPNMYFKNDELEFAKSFIGIVSIFCLCATLFTFLTFLIDVRRFRYPERPIIYYSVCYSIVSLMYFIGFLLGNSTACNKADEKLELGDTVVLGSQNKACTVLFMFLYFFTMAGTVWWVILTITWFLAAGRKWSCEAIEQKAVWFHAVAWGIPGFLTVMLLAMNKVEGDNISGVCFVGLYDLDASRYFVLLPLCFCVFVGLSLLLAGIISLNHVRQVIQHDGRNQEKLKKFMIRIGVFSGLYLVPLVMLLGCYVYEQVNRITWEITWVSDHCRQYHIPCPYQAKTETRPELALFMMKYLMTLIVGISAVFWVGSKKTCTEWAGFFKRNRKKDPISESRRVLQESCEFFLKHNSKVKHKKKHYKPSSHKLKVISKSMGTSTGATANHGTSAVAITNHDYLGQETLTEIQTSPETSVREVRADRASPPKAREQDGEDPASSAASSSRLCGEQTDRKGRAGNVKDKSRVPASARSEGRVTPKNDGTETSPAPSNSLQAPSSSEPGSLKGSTSLLVHSASGVRKEQGTGSHSDT

Target ORF information:

RefSeq Version	NM_001315750.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa frizzled class receptor 6 (FZD6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001315750.1

ORF Insert Sequence:

ATGGAAATGT TTACATTTTT GTGGACATGT ATTTTTCTGC CCCTCATAAG AGGGCACAGT 
CTTTTCACCT GTGAACCAAT TACTGTTCCC AGATGTATGA AAATGGCCTA CAACATGACA 
TTTTTCCCTA ATTTGATGGG TCATTATGAC CAGAGTATTG CTGCTGTGGA AATGGAGCCT 
TTTCTCCCTC TTGCGAATCT GGAATGTTCA CCAAATGTTG AAACTTTCCT TTGCAAAGCT 
TTTGTACCAA CCTGCACAGA GCAAATTGAT GTGGTTCCAC CCTGTCGTAA ATTTTGTGAG 
AAAGTATATT CTGATTGCAA AAAATTAATG GATACTTTTG GGATCCGATG GCCTGAGGAA 
CTTGAATGTA ACAGGTTACA ATACTGTGAC GAACCTGTTC CTGCAACTTT TGATCCACAC 
ACAGATTTTC TTAGTCCTCA GAAGAAAATA GAACCAGTCC AAAGAGACAT TGGATTTTGG 
TGTCCAAGGC ATCTTAAGAC TTCTGGGGGA CAAGGCTATA AGTTTCTTGG AATTGACCAG 
TGTGCACCTC CATGCCCCAA CATGTATTTT AAGAATGATG AGTTGGAGTT TGCAAAAAGT 
TTTATTGGAA TAGTTTCAAT ATTTTGTCTT TGTGCAACTC TGTTCACATT CCTTACTTTT 
TTAATTGATG TTAGAAGATT CAGATACCCA GAGAGACCAA TTATATATTA CTCTGTCTGT 
TATAGCATTG TATCTCTTAT GTACTTTATT GGATTCTTGC TGGGCAATAG TACAGCCTGC 
AATAAGGCAG ACGAGAAGCT AGAACTTGGT GACACAGTTG TCCTAGGCTC TCAAAATAAG 
GCTTGCACCG TTTTGTTTAT GTTTTTGTAT TTTTTCACAA TGGCTGGCAC TGTGTGGTGG 
GTGATTCTTA CCATTACTTG GTTCTTAGCT GCTGGGAGAA AGTGGAGTTG TGAAGCCATT 
GAGCAAAAAG CAGTGTGGTT TCATGCCGTG GCCTGGGGAA TACCAGGTTT TCTGACCGTT 
ATGCTTCTTG CTATGAACAA AGTGGAAGGA GACAATATTA GTGGAGTTTG CTTTGTCGGT 
CTTTATGACC TGGATGCTTC TCGCTACTTT GTACTCTTGC CGCTGTGCTT TTGTGTGTTT 
GTTGGGCTGT CTCTTCTTTT AGCTGGCATT ATTTCCTTAA ATCATGTTCG ACAAGTTATA 
CAGCATGATG GCCGGAACCA AGAGAAGCTA AAGAAATTTA TGATTCGAAT CGGAGTCTTC 
AGTGGCCTGT ATCTTGTGCC ATTAGTGATG CTTCTTGGAT GTTATGTCTA TGAGCAAGTG 
AACAGGATCA CCTGGGAGAT AACTTGGGTC TCTGACCATT GTCGTCAGTA CCATATACCA 
TGTCCTTATC AGGCAAAAAC AGAAACTCGA CCAGAATTGG CATTATTTAT GATGAAATAT 
TTGATGACTT TAATTGTTGG CATCTCTGCT GTCTTCTGGG TCGGAAGCAA AAAGACATGC 
ACAGAATGGG CTGGGTTTTT TAAACGAAAT CGCAAGAAAG ATCCAATCAG TGAAAGCCGA 
AGAGTACTAC AAGAGTCATG TGAGTTTTTC TTAAAGCACA ATTCTAAAGT TAAACACAAA 
AAGAAGCACT ACAAACCAAG TTCACACAAG CTGAAGGTCA TTTCCAAATC CATGGGAACC 
AGCACAGGAG CGACAGCAAA TCATGGCACT TCTGCAGTAG CAATCACTAA CCATGACTAC 
TTAGGACAAG AAACTTTGAC AGAAATACAA ACCTCGCCAG AAACATCAGT GAGGGAAGTG 
AGAGCGGACA GAGCTAGCCC GCCCAAAGCG AGAGAACAGG ACGGTGAGGA CCCTGCCTCC 
TCAGCAGCAT CCAGCTCCAG ACTCTGCGGG GAACAGACCG ACAGGAAGGG CCGGGCAGGC 
AATGTGAAGG ATAAGAGCCG AGTGCCGGCA AGTGCGCGGA GTGAAGGAAG GGTAACTCCA 
AAGAACGATG GCACTGAAAC CAGCCCGGCA CCGAGCAACA GCCTGCAGGC CCCCAGTTCT 
TCAGAGCCAG GCAGCCTCAA AGGCTCCACA TCACTGCTTG TTCACTCGGC TTCAGGAGTT 
AGAAAAGAGC AGGGCACTGG CAGTCACTCG GATACTTGA