wwp2 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	wwp2
Entrez Gene ID	111238830
Full Name	WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022768528.1	XP_022624249.1	NEDD4-like E3 ubiquitin-protein ligase WWP2 isoform X1
XM_022768529.1	XP_022624250.1	NEDD4-like E3 ubiquitin-protein ligase WWP2 isoform X2

Pathways from BioSystems
Gene Ontology

The following wwp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wwp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe197563	XM_022768528.1 Latest version!	Seriola dumerili WW domain containing E3 ubiquitin protein ligase 2 (wwp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OSe197564	XM_022768529.1 Latest version!	Seriola dumerili WW domain containing E3 ubiquitin protein ligase 2 (wwp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wwp2 ( XM_022768528.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022624249.1 Seriola dumerili WW domain containing E3 ubiquitin protein ligase 2 (wwp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe197563
Clone ID Related Accession (Same CDS sequence)	XM_022768528.1
Accession Version	XM_022768528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019175945.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG CCAGTGTGAG CAGACCTGCC GGTTCCCAGA CCACTGAAAA GTCTCAGTTG 
ATCTTGAAAG TGGTTTCAGC GAAGCCCCAG TCTCCCAAGC TGCCAAACCG CCACTCTCGA 
CTCAGCTCCT TTGTGGAGGT GACTGCCGAC GGTCTGACCA GTGAAACCAA AAAAACAGGC 
AAACGCAGCG GACATCTTGA GCTGCAGTGG AATGAAGACC TCACCCTTAA TGTAACGCCT 
CAGAGTCATC TGGATCTGAA GCTTTGGAGC TGCCACACCC TGAGGAAGGA GCTGCTGGGC 
AGTGCCACCA TAGACCTGTT GGACACACTG CGCACACATG ATGGCAAGAT GGAGAACGTC 
CAGCTGAGTC TGGTGCTACA GACGGAGAAC AAAGGCTTGG TTGTAGCGGG AGGCGAGCTC 
AACGTCTGTC TGGACGGCAT GGTTGTTGAT CTGGGCTCGC TGCCCAATGG CAGCACGGCA 
GCAGACAAGA TACTTCAGTC AGACAGACCC AACCTGAGGA GAACACAGAG TGAGGAGACT 
CCCAGTGCTG TTGGATCCCA GAGCAGGAGT AACAGGCACT CGGTGGGGGG TTCGCAGAGT 
GGCTCCCCAG GGTGCTCCAG AGGGGGGTTT CTGACTAATG GGGAGCTGAC TGAGGAGCAG 
ATCCCTGGGC CGGGCTCGTC TGGGCGCCGT CAGTCCTCCA AGAGTCAAGG AAGCCCAGGA 
AACAGTGCAG ACAAAGTTGA GTTGACGTCA AATGGGCTGG AGAACAGTCG AGGGGGCGTC 
GCCCGCCACA CACCTCCTCC ACCCTCTCCG TCCGGCCGCT CCCCGGCCGC CAGCAGCAGC 
AACGGTAGCC CCTCTCCAGG TCAGGACGCC CTGGGTCCGA CAACCCCGGC GGCGCCCACC 
CCCAGTGCCA CCTCCAGCAC AGAGCAGGCC AGCAGCAGCA GCACCACGAC AGAGTCGACC 
ACAGAATCAC TCCCGGCAGG CTGGGAGCAG AGGGTGTTGC CTCATGGCAG AGTGTACTAC 
GTCGACCACA ACACCAAAAC CACAACCTGG GAGAGACCAC TGCCACCAGG CTGGGAGAAG 
CGGGTGGACC AGCAGGGCAG ATTCTACTAT GTGGACCACA ACACCAGAAC CACCACATGG 
CAGAGGCCCA CGGCCGAGTC GGTGCGCAAC TACCAGCAGT GGCAGAGCCA GCGCAGCCAG 
CTGCAGGGCG CCATGCACCA GTTCAGCCAG CGTTTCCTCT ACCAGCCGTC TGGTGCTCCG 
CTGGAAAATG ACCCTCTGGG CCCGCTGCCT CCTGGATGGG AGAAGCGTCA GGACAATGGC 
AGAGTGTATT TTGTCAACCA CAACACACGG ACAACACAGT GGGACGACCC GCGGACCCAA 
GGGATGATCA AGGAGCACCC CCTGCCCCCG GGCTGGGAGA TGAAGTACAC CGCAGAGGGC 
GTCCGATATT TTGTGGACCA CAACTCACGC ACCACCACCT TTAAAGATCC CCGACCTGGG 
TTTGAGTCAG GATCACGGCA GGGCGGTTCA CCTGGCGCCT ACGACCGCAG CTTCAGGTGG 
AAATACCACC AGTTCCGTTT CCTTTGCCAT TCCAACGCAT TGCCCAGCCA TGTGAAGATC 
TCTGTGTCCA GACAGACGCT GTTTGAGGAC TCATTTCAGC AGATCATGAA TGTGAAGCCG 
TACGACCTTC GCCGCAGACT CTACATCATC ATGAGAGGAG AGGAGGGGCT GGACTACGGC 
GGCATCGCCA GGGAGTGGTT CTTCCTGCTG TCCCATGAGG TCCTGAACCC CATGTACTGT 
CTGTTTGAAT ACGCCGGCAA GAACAACTAC TGTCTGCAGA TCAACCCGGC TTCCTCCATC 
AACCCCGACC ACCTCACATA CTTCCGCTTC ATTGGACGCT TCATTGCTAT GGCTTTGTAC 
CACGGAAAGT TCATCGACAC CGGCTTCACG CTGCCATTCT ACAAGCGAAT GCTGGACAAG 
AAGCCCACTC TCAAAGACCT GGAGTCCATA GACCCTGAGT TCTATAACTC CATCATGTGG 
GTCAAAGACA ACAACCTGGA GGAGTGTGGT GTGGAGCTGT ATTTTGCACA GGACATGGAG 
ATCCTTGGGA AAGTTTCCAC TCACCAGCTG AAGGATGATG GAGAGAACGA GCTGGTCACG 
GAGGAAAACA AGGAAGAGTA CATCAGCCTG CTGACAGACT GGAGGTTCAC CCGCGGCGTG 
GAGGAGCAGA CCAAAGCTTT CCTGGACGGC TTCAACGAGG TGGTTCCCCT GGAGTGGCTT 
CGCTACTTTG ATGAGAAGGA GCTGGAGCTG ATGCTCTGTG GGATGCAGGA GATCGACTTA 
GCCGACTGGC AGAAGAACAC CATCTACAGA CATTACACCA AGAACAGCAA GCAGATCCAC 
TGGTTCTGGC AGGTGGTGAA AGAGATGGAC AATGAGAAGA GAATCCGTCT GCTTCAGTTT 
GTGACGGGAA CCTGTCGGCT GCCTGTCGGA GGCTTCGCTG AGCTCATAGG AAGTAACGGT 
CCCCAGAAGT TCTGCATAGA CAAGGTGGGG AAGGAGACTT GGCTTCCAAG AAGCCACACA 
TGCTTCAACC GTCTGGATCT GCCACCCTAC AGAAGCCTGG AGCAGCTCCG AGAGAAACTC 
CTGTTTGCCA TCGAGGAAAC AGAGGGGTTT GGACAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022624249.1
CDS	61..2742
Translation	MAAASVSRPAGSQTTEKSQLILKVVSAKPQSPKLPNRHSRLSSFVEVTADGLTSETKKTGKRSGHLELQWNEDLTLNVTPQSHLDLKLWSCHTLRKELLGSATIDLLDTLRTHDGKMENVQLSLVLQTENKGLVVAGGELNVCLDGMVVDLGSLPNGSTAADKILQSDRPNLRRTQSEETPSAVGSQSRSNRHSVGGSQSGSPGCSRGGFLTNGELTEEQIPGPGSSGRRQSSKSQGSPGNSADKVELTSNGLENSRGGVARHTPPPPSPSGRSPAASSSNGSPSPGQDALGPTTPAAPTPSATSSTEQASSSSTTTESTTESLPAGWEQRVLPHGRVYYVDHNTKTTTWERPLPPGWEKRVDQQGRFYYVDHNTRTTTWQRPTAESVRNYQQWQSQRSQLQGAMHQFSQRFLYQPSGAPLENDPLGPLPPGWEKRQDNGRVYFVNHNTRTTQWDDPRTQGMIKEHPLPPGWEMKYTAEGVRYFVDHNSRTTTFKDPRPGFESGSRQGGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNVKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLDKKPTLKDLESIDPEFYNSIMWVKDNNLEECGVELYFAQDMEILGKVSTHQLKDDGENELVTEENKEEYISLLTDWRFTRGVEEQTKAFLDGFNEVVPLEWLRYFDEKELELMLCGMQEIDLADWQKNTIYRHYTKNSKQIHWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYRSLEQLREKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_022768528.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili WW domain containing E3 ubiquitin protein ligase 2 (wwp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022768528.1

ORF Insert Sequence:

ATGGCAGCTG CCAGTGTGAG CAGACCTGCC GGTTCCCAGA CCACTGAAAA GTCTCAGTTG 
ATCTTGAAAG TGGTTTCAGC GAAGCCCCAG TCTCCCAAGC TGCCAAACCG CCACTCTCGA 
CTCAGCTCCT TTGTGGAGGT GACTGCCGAC GGTCTGACCA GTGAAACCAA AAAAACAGGC 
AAACGCAGCG GACATCTTGA GCTGCAGTGG AATGAAGACC TCACCCTTAA TGTAACGCCT 
CAGAGTCATC TGGATCTGAA GCTTTGGAGC TGCCACACCC TGAGGAAGGA GCTGCTGGGC 
AGTGCCACCA TAGACCTGTT GGACACACTG CGCACACATG ATGGCAAGAT GGAGAACGTC 
CAGCTGAGTC TGGTGCTACA GACGGAGAAC AAAGGCTTGG TTGTAGCGGG AGGCGAGCTC 
AACGTCTGTC TGGACGGCAT GGTTGTTGAT CTGGGCTCGC TGCCCAATGG CAGCACGGCA 
GCAGACAAGA TACTTCAGTC AGACAGACCC AACCTGAGGA GAACACAGAG TGAGGAGACT 
CCCAGTGCTG TTGGATCCCA GAGCAGGAGT AACAGGCACT CGGTGGGGGG TTCGCAGAGT 
GGCTCCCCAG GGTGCTCCAG AGGGGGGTTT CTGACTAATG GGGAGCTGAC TGAGGAGCAG 
ATCCCTGGGC CGGGCTCGTC TGGGCGCCGT CAGTCCTCCA AGAGTCAAGG AAGCCCAGGA 
AACAGTGCAG ACAAAGTTGA GTTGACGTCA AATGGGCTGG AGAACAGTCG AGGGGGCGTC 
GCCCGCCACA CACCTCCTCC ACCCTCTCCG TCCGGCCGCT CCCCGGCCGC CAGCAGCAGC 
AACGGTAGCC CCTCTCCAGG TCAGGACGCC CTGGGTCCGA CAACCCCGGC GGCGCCCACC 
CCCAGTGCCA CCTCCAGCAC AGAGCAGGCC AGCAGCAGCA GCACCACGAC AGAGTCGACC 
ACAGAATCAC TCCCGGCAGG CTGGGAGCAG AGGGTGTTGC CTCATGGCAG AGTGTACTAC 
GTCGACCACA ACACCAAAAC CACAACCTGG GAGAGACCAC TGCCACCAGG CTGGGAGAAG 
CGGGTGGACC AGCAGGGCAG ATTCTACTAT GTGGACCACA ACACCAGAAC CACCACATGG 
CAGAGGCCCA CGGCCGAGTC GGTGCGCAAC TACCAGCAGT GGCAGAGCCA GCGCAGCCAG 
CTGCAGGGCG CCATGCACCA GTTCAGCCAG CGTTTCCTCT ACCAGCCGTC TGGTGCTCCG 
CTGGAAAATG ACCCTCTGGG CCCGCTGCCT CCTGGATGGG AGAAGCGTCA GGACAATGGC 
AGAGTGTATT TTGTCAACCA CAACACACGG ACAACACAGT GGGACGACCC GCGGACCCAA 
GGGATGATCA AGGAGCACCC CCTGCCCCCG GGCTGGGAGA TGAAGTACAC CGCAGAGGGC 
GTCCGATATT TTGTGGACCA CAACTCACGC ACCACCACCT TTAAAGATCC CCGACCTGGG 
TTTGAGTCAG GATCACGGCA GGGCGGTTCA CCTGGCGCCT ACGACCGCAG CTTCAGGTGG 
AAATACCACC AGTTCCGTTT CCTTTGCCAT TCCAACGCAT TGCCCAGCCA TGTGAAGATC 
TCTGTGTCCA GACAGACGCT GTTTGAGGAC TCATTTCAGC AGATCATGAA TGTGAAGCCG 
TACGACCTTC GCCGCAGACT CTACATCATC ATGAGAGGAG AGGAGGGGCT GGACTACGGC 
GGCATCGCCA GGGAGTGGTT CTTCCTGCTG TCCCATGAGG TCCTGAACCC CATGTACTGT 
CTGTTTGAAT ACGCCGGCAA GAACAACTAC TGTCTGCAGA TCAACCCGGC TTCCTCCATC 
AACCCCGACC ACCTCACATA CTTCCGCTTC ATTGGACGCT TCATTGCTAT GGCTTTGTAC 
CACGGAAAGT TCATCGACAC CGGCTTCACG CTGCCATTCT ACAAGCGAAT GCTGGACAAG 
AAGCCCACTC TCAAAGACCT GGAGTCCATA GACCCTGAGT TCTATAACTC CATCATGTGG 
GTCAAAGACA ACAACCTGGA GGAGTGTGGT GTGGAGCTGT ATTTTGCACA GGACATGGAG 
ATCCTTGGGA AAGTTTCCAC TCACCAGCTG AAGGATGATG GAGAGAACGA GCTGGTCACG 
GAGGAAAACA AGGAAGAGTA CATCAGCCTG CTGACAGACT GGAGGTTCAC CCGCGGCGTG 
GAGGAGCAGA CCAAAGCTTT CCTGGACGGC TTCAACGAGG TGGTTCCCCT GGAGTGGCTT 
CGCTACTTTG ATGAGAAGGA GCTGGAGCTG ATGCTCTGTG GGATGCAGGA GATCGACTTA 
GCCGACTGGC AGAAGAACAC CATCTACAGA CATTACACCA AGAACAGCAA GCAGATCCAC 
TGGTTCTGGC AGGTGGTGAA AGAGATGGAC AATGAGAAGA GAATCCGTCT GCTTCAGTTT 
GTGACGGGAA CCTGTCGGCT GCCTGTCGGA GGCTTCGCTG AGCTCATAGG AAGTAACGGT 
CCCCAGAAGT TCTGCATAGA CAAGGTGGGG AAGGAGACTT GGCTTCCAAG AAGCCACACA 
TGCTTCAACC GTCTGGATCT GCCACCCTAC AGAAGCCTGG AGCAGCTCCG AGAGAAACTC 
CTGTTTGCCA TCGAGGAAAC AGAGGGGTTT GGACAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp2 ( XM_022768529.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022624250.1 Seriola dumerili WW domain containing E3 ubiquitin protein ligase 2 (wwp2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe197564
Clone ID Related Accession (Same CDS sequence)	XM_022768529.1
Accession Version	XM_022768529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019175945.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG CCAGTGTGAG CAGACCTGCC GGTTCCCAGA CCACTGAAAA GTCTCAGTTG 
ATCTTGAAAG TGGTTTCAGC GAAGCCCCAG TCTCCCAAGC TGCCAAACCG CCACTCTCGA 
CTCAGCTCCT TTGTGGAGGT GACTGCCGAC GGTCTGACCA GTGAAACCAA AAAAACAGGC 
AAACGCAGCG GACATCTTGA GCTGCAGTGG AATGAAGACC TCACCCTTAA TGTAACGCCT 
CAGAGTCATC TGGATCTGAA GCTTTGGAGC TGCCACACCC TGAGGAAGGA GCTGCTGGGC 
AGTGCCACCA TAGACCTGTT GGACACACTG CGCACACATG ATGGCAAGAT GGAGAACGTC 
CAGCTGAGTC TGGTGCTACA GACGGAGAAC AAAGGCTTGG TTGTAGCGGG AGGCGAGCTC 
AACGTCTGTC TGGACGGCAT GGTTGTTGAT CTGGGCTCGC TGCCCAATGG CAGCACGGCA 
GCAGACAAGA TACTTCAGTC AGACAGACCC AACCTGAGGA GAACACAGAG TGAGGAGACT 
CCCAGTGCTG TTGGATCCCA GAGCAGGAGT AACAGGCACT CGGTGGGGGG TTCGCAGAGT 
GGCTCCCCAG GGTGCTCCAG AGGGGGGTTT CTGACTAATG GGGAGCTGAC TGAGGAGCAG 
ATCCCTGGGC CGGGCTCGTC TGGGCGCCGT CAGTCCTCCA AGAGTCAAGG AAGCCCAGGA 
AACAGTGCAG ACAAAGTTGA GTTGACGTCA AATGGGCTGG AGAACAGTCG AGGGGGCGTC 
GCCCGCCACA CACCTCCTCC ACCCTCTCCG TCCGGCCGCT CCCCGGCCGC CAGCAGCAGC 
AACGGTAGCC CCTCTCCAGG TCAGGACGCC CTGGGTCCGA CAACCCCGGC GGCGCCCACC 
CCCAGTGCCA CCTCCAGCAC AGAGCAGGCC AGCAGCAGCA GCACCACGAC AGAGTCGACC 
ACAGAATCAC TCCCGGCAGG CTGGGAGCAG AGGGTGTTGC CTCATGGCAG AGTGTACTAC 
GTCGACCACA ACACCAAAAC CACAACCTGG GAGAGACCAC TGCCACCAGG CTGGGAGAAG 
CGGGTGGACC AGCAGGGCAG ATTCTACTAT GTGGACCACA ACACCAGAAC CACCACATGG 
CAGAGGCCCA CGGCCGAGTC GGTGCGCAAC TACCAGCAGT GGCAGAGCCA GCGCAGCCAG 
CTGCAGGGCG CCATGCACCA GTTCAGCCAG CGTTTCCTCT ACCAGCCGTC TGGTGCTCCG 
CTGGAAAATG ACCCTCTGGG CCCGCTGCCT CCTGGATGGG AGAAGCGTCA GGACAATGGC 
AGAGTGTATT TTGTCAACCA CAACACACGG ACAACACAGT GGGACGACCC GCGGACCCAA 
GGATCACGGC AGGGCGGTTC ACCTGGCGCC TACGACCGCA GCTTCAGGTG GAAATACCAC 
CAGTTCCGTT TCCTTTGCCA TTCCAACGCA TTGCCCAGCC ATGTGAAGAT CTCTGTGTCC 
AGACAGACGC TGTTTGAGGA CTCATTTCAG CAGATCATGA ATGTGAAGCC GTACGACCTT 
CGCCGCAGAC TCTACATCAT CATGAGAGGA GAGGAGGGGC TGGACTACGG CGGCATCGCC 
AGGGAGTGGT TCTTCCTGCT GTCCCATGAG GTCCTGAACC CCATGTACTG TCTGTTTGAA 
TACGCCGGCA AGAACAACTA CTGTCTGCAG ATCAACCCGG CTTCCTCCAT CAACCCCGAC 
CACCTCACAT ACTTCCGCTT CATTGGACGC TTCATTGCTA TGGCTTTGTA CCACGGAAAG 
TTCATCGACA CCGGCTTCAC GCTGCCATTC TACAAGCGAA TGCTGGACAA GAAGCCCACT 
CTCAAAGACC TGGAGTCCAT AGACCCTGAG TTCTATAACT CCATCATGTG GGTCAAAGAC 
AACAACCTGG AGGAGTGTGG TGTGGAGCTG TATTTTGCAC AGGACATGGA GATCCTTGGG 
AAAGTTTCCA CTCACCAGCT GAAGGATGAT GGAGAGAACG AGCTGGTCAC GGAGGAAAAC 
AAGGAAGAGT ACATCAGCCT GCTGACAGAC TGGAGGTTCA CCCGCGGCGT GGAGGAGCAG 
ACCAAAGCTT TCCTGGACGG CTTCAACGAG GTGGTTCCCC TGGAGTGGCT TCGCTACTTT 
GATGAGAAGG AGCTGGAGCT GATGCTCTGT GGGATGCAGG AGATCGACTT AGCCGACTGG 
CAGAAGAACA CCATCTACAG ACATTACACC AAGAACAGCA AGCAGATCCA CTGGTTCTGG 
CAGGTGGTGA AAGAGATGGA CAATGAGAAG AGAATCCGTC TGCTTCAGTT TGTGACGGGA 
ACCTGTCGGC TGCCTGTCGG AGGCTTCGCT GAGCTCATAG GAAGTAACGG TCCCCAGAAG 
TTCTGCATAG ACAAGGTGGG GAAGGAGACT TGGCTTCCAA GAAGCCACAC ATGCTTCAAC 
CGTCTGGATC TGCCACCCTA CAGAAGCCTG GAGCAGCTCC GAGAGAAACT CCTGTTTGCC 
ATCGAGGAAA CAGAGGGGTT TGGACAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022624250.1
CDS	61..2613
Translation	MAAASVSRPAGSQTTEKSQLILKVVSAKPQSPKLPNRHSRLSSFVEVTADGLTSETKKTGKRSGHLELQWNEDLTLNVTPQSHLDLKLWSCHTLRKELLGSATIDLLDTLRTHDGKMENVQLSLVLQTENKGLVVAGGELNVCLDGMVVDLGSLPNGSTAADKILQSDRPNLRRTQSEETPSAVGSQSRSNRHSVGGSQSGSPGCSRGGFLTNGELTEEQIPGPGSSGRRQSSKSQGSPGNSADKVELTSNGLENSRGGVARHTPPPPSPSGRSPAASSSNGSPSPGQDALGPTTPAAPTPSATSSTEQASSSSTTTESTTESLPAGWEQRVLPHGRVYYVDHNTKTTTWERPLPPGWEKRVDQQGRFYYVDHNTRTTTWQRPTAESVRNYQQWQSQRSQLQGAMHQFSQRFLYQPSGAPLENDPLGPLPPGWEKRQDNGRVYFVNHNTRTTQWDDPRTQGSRQGGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNVKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLDKKPTLKDLESIDPEFYNSIMWVKDNNLEECGVELYFAQDMEILGKVSTHQLKDDGENELVTEENKEEYISLLTDWRFTRGVEEQTKAFLDGFNEVVPLEWLRYFDEKELELMLCGMQEIDLADWQKNTIYRHYTKNSKQIHWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYRSLEQLREKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_022768529.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili WW domain containing E3 ubiquitin protein ligase 2 (wwp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022768529.1

ORF Insert Sequence:

ATGGCAGCTG CCAGTGTGAG CAGACCTGCC GGTTCCCAGA CCACTGAAAA GTCTCAGTTG 
ATCTTGAAAG TGGTTTCAGC GAAGCCCCAG TCTCCCAAGC TGCCAAACCG CCACTCTCGA 
CTCAGCTCCT TTGTGGAGGT GACTGCCGAC GGTCTGACCA GTGAAACCAA AAAAACAGGC 
AAACGCAGCG GACATCTTGA GCTGCAGTGG AATGAAGACC TCACCCTTAA TGTAACGCCT 
CAGAGTCATC TGGATCTGAA GCTTTGGAGC TGCCACACCC TGAGGAAGGA GCTGCTGGGC 
AGTGCCACCA TAGACCTGTT GGACACACTG CGCACACATG ATGGCAAGAT GGAGAACGTC 
CAGCTGAGTC TGGTGCTACA GACGGAGAAC AAAGGCTTGG TTGTAGCGGG AGGCGAGCTC 
AACGTCTGTC TGGACGGCAT GGTTGTTGAT CTGGGCTCGC TGCCCAATGG CAGCACGGCA 
GCAGACAAGA TACTTCAGTC AGACAGACCC AACCTGAGGA GAACACAGAG TGAGGAGACT 
CCCAGTGCTG TTGGATCCCA GAGCAGGAGT AACAGGCACT CGGTGGGGGG TTCGCAGAGT 
GGCTCCCCAG GGTGCTCCAG AGGGGGGTTT CTGACTAATG GGGAGCTGAC TGAGGAGCAG 
ATCCCTGGGC CGGGCTCGTC TGGGCGCCGT CAGTCCTCCA AGAGTCAAGG AAGCCCAGGA 
AACAGTGCAG ACAAAGTTGA GTTGACGTCA AATGGGCTGG AGAACAGTCG AGGGGGCGTC 
GCCCGCCACA CACCTCCTCC ACCCTCTCCG TCCGGCCGCT CCCCGGCCGC CAGCAGCAGC 
AACGGTAGCC CCTCTCCAGG TCAGGACGCC CTGGGTCCGA CAACCCCGGC GGCGCCCACC 
CCCAGTGCCA CCTCCAGCAC AGAGCAGGCC AGCAGCAGCA GCACCACGAC AGAGTCGACC 
ACAGAATCAC TCCCGGCAGG CTGGGAGCAG AGGGTGTTGC CTCATGGCAG AGTGTACTAC 
GTCGACCACA ACACCAAAAC CACAACCTGG GAGAGACCAC TGCCACCAGG CTGGGAGAAG 
CGGGTGGACC AGCAGGGCAG ATTCTACTAT GTGGACCACA ACACCAGAAC CACCACATGG 
CAGAGGCCCA CGGCCGAGTC GGTGCGCAAC TACCAGCAGT GGCAGAGCCA GCGCAGCCAG 
CTGCAGGGCG CCATGCACCA GTTCAGCCAG CGTTTCCTCT ACCAGCCGTC TGGTGCTCCG 
CTGGAAAATG ACCCTCTGGG CCCGCTGCCT CCTGGATGGG AGAAGCGTCA GGACAATGGC 
AGAGTGTATT TTGTCAACCA CAACACACGG ACAACACAGT GGGACGACCC GCGGACCCAA 
GGATCACGGC AGGGCGGTTC ACCTGGCGCC TACGACCGCA GCTTCAGGTG GAAATACCAC 
CAGTTCCGTT TCCTTTGCCA TTCCAACGCA TTGCCCAGCC ATGTGAAGAT CTCTGTGTCC 
AGACAGACGC TGTTTGAGGA CTCATTTCAG CAGATCATGA ATGTGAAGCC GTACGACCTT 
CGCCGCAGAC TCTACATCAT CATGAGAGGA GAGGAGGGGC TGGACTACGG CGGCATCGCC 
AGGGAGTGGT TCTTCCTGCT GTCCCATGAG GTCCTGAACC CCATGTACTG TCTGTTTGAA 
TACGCCGGCA AGAACAACTA CTGTCTGCAG ATCAACCCGG CTTCCTCCAT CAACCCCGAC 
CACCTCACAT ACTTCCGCTT CATTGGACGC TTCATTGCTA TGGCTTTGTA CCACGGAAAG 
TTCATCGACA CCGGCTTCAC GCTGCCATTC TACAAGCGAA TGCTGGACAA GAAGCCCACT 
CTCAAAGACC TGGAGTCCAT AGACCCTGAG TTCTATAACT CCATCATGTG GGTCAAAGAC 
AACAACCTGG AGGAGTGTGG TGTGGAGCTG TATTTTGCAC AGGACATGGA GATCCTTGGG 
AAAGTTTCCA CTCACCAGCT GAAGGATGAT GGAGAGAACG AGCTGGTCAC GGAGGAAAAC 
AAGGAAGAGT ACATCAGCCT GCTGACAGAC TGGAGGTTCA CCCGCGGCGT GGAGGAGCAG 
ACCAAAGCTT TCCTGGACGG CTTCAACGAG GTGGTTCCCC TGGAGTGGCT TCGCTACTTT 
GATGAGAAGG AGCTGGAGCT GATGCTCTGT GGGATGCAGG AGATCGACTT AGCCGACTGG 
CAGAAGAACA CCATCTACAG ACATTACACC AAGAACAGCA AGCAGATCCA CTGGTTCTGG 
CAGGTGGTGA AAGAGATGGA CAATGAGAAG AGAATCCGTC TGCTTCAGTT TGTGACGGGA 
ACCTGTCGGC TGCCTGTCGG AGGCTTCGCT GAGCTCATAG GAAGTAACGG TCCCCAGAAG 
TTCTGCATAG ACAAGGTGGG GAAGGAGACT TGGCTTCCAA GAAGCCACAC ATGCTTCAAC 
CGTCTGGATC TGCCACCCTA CAGAAGCCTG GAGCAGCTCC GAGAGAAACT CCTGTTTGCC 
ATCGAGGAAA CAGAGGGGTT TGGACAGGAG TGA