dennd2a cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	dennd2a
Entrez Gene ID	111229800
Full Name	DENN domain containing 2A
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022756268.1	XP_022611989.1	DENN domain-containing protein 2A
XM_022756269.1	XP_022611990.1	DENN domain-containing protein 2A

Pathways from BioSystems
Gene Ontology

The following dennd2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dennd2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe202592	XM_022756269.1 Latest version!	Seriola dumerili DENN domain containing 2A (dennd2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSe202592	XM_022756268.1 Latest version!	Seriola dumerili DENN domain containing 2A (dennd2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dennd2a ( XM_022756268.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022611989.1 Seriola dumerili DENN domain containing 2A (dennd2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe202592
Clone ID Related Accession (Same CDS sequence)	XM_022756268.1 , XM_022756269.1
Accession Version	XM_022756268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	DENN domain-containing protein 2A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174497.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG CTAGCACCAT GACTGGTGGG AGGGCCCTGC TGCTCTGTAC AAACACTGAC 
AACTGTATCT ACCAATCAGT CAACGGGCTC CAGTGCTGTG GCTTGAAGGT TGGGGAGGCT 
CCCTCATCTT CGGTGCCCCA GCCCCAAGAG GTGTTCGGGT CACCGGGGAA CAGAAACGGG 
AGGGACACTG TGTCTTCCAA GCGTCCCCTC TCACCCTTAC TCAGCCTGAG CGACAGCCCG 
GGTCCTCCTG ACACCATGTT GGCTCACAGG AGAGCCAGCA ACACCACCGA CCCGCGGACA 
TCCAGCATGG AGAACGGACT GTGTGGCAAA CTGAACCACA GCAAGAGCCC AGCAGGAGTC 
AAGAGTGCAA GCATCCGCGA TAAGATCTCA CAATGGGAGG GCAAAAAGGA ACCTGCCCAA 
CTTACCTCTA CAGGGACATG TCCACTGAGC ACGACACAAA AAGAAGTTGA GACAGTGAGG 
AAAAAGGAAT CTAAAGTTTC AGAGGTCCAA AGGACAGATT GCAAGCGGTT TGTCAGCTGG 
GACAGACAAG ACTCAGGGAA GGAGAATGTC GGAAAGCTTG GGGATTCAAG GCCGAAATCT 
CCAGAAGGCC CAACAAACAA AGACAGAGAA GTGATACTAG AGAGAGGGTT TCGGGCTTCT 
AAGCCAACAG AGCAACCCCT CGACAAGAAA ACTGTTTTAA CTCATGTTAA GAAACTGGAG 
AAGGCAACAA AGGAAGTTCC TGACAGACCT TCACTTGCAT TTCCAGGGAA TTACTTCTGT 
CCTCCTTCAA AGGAGGAGGT GGAAGAAACA GAAAAGAGGG CCGGCGAGCC CATTTTTGGG 
AGTTTTGACG TCCCTCGGCC CAGTGGGTCA CGGAGGAGGA GGGAGGGGGA TCCAGAGAAC 
GTATACAGCG AGCCAGGTGC TCCATCTATA AACCCTCTAC CTAAACCTCA GAGAACCTTC 
CAGCACCACA CACCGCCCGC TACCCCAACT TCAGGGGCCG GCTCGGGGAA GGGAAAGAGG 
AACTTGCCCC CTTTGCCCTC CATTCCTCCT CCACCTTTAC CAACATGCCC ACCACCTGGA 
GTGTGCAGGA GACCCTGGGC TGACAAACCC CGGGACAGCA GTAACAGGAA GTCGTATGAA 
TTCGAGGATC TGCTCCAGTC GTCTACAGAG AGCTGCAGGG TGGACTGGTA CGCTCAGTCC 
AGACTGGGCC TAACACGCAC TTTATCAGAA GAGAATGTCT ACGAGGACAT AATAGATCCT 
CCATCCAAGG AGAACCCTTA TGAAGATATA GAGCTGGAGA GAAGTTGTTT GGGAAGCAAA 
TGTGTTTCAC CTGCCTCCTC ATCTCCTGTC CCTGACACGC CAACTAAGCT CTCCTCCAAG 
CCCGGCTTCT TCAGACAAAA CTCAGAACGG CGAAGTTTTA AGCTCCTCGA ACTACGCAAG 
ACCGGCAGGG ACACTGGCAT CTCCTCACCC TCCCGTGTCA GCCCGCCCTC CACACCCAGC 
AGCCCCGACG ACACCCCCTG CCTCTCAGGA GACCCGTACA ATCGCAGACG GAGGAAAATC 
CCCAAGATGG TGCTGAAAAT CAATGGCATC TTTGAGGCAC GAAGAGGGAA GAAACGCATG 
AAGAGAGTGT CTCAGTCGAC CGAGTCCAGC TCAGGGAGAG TGACAGATGA GAACAGTGAG 
TCGGAAAGTG ACACGGAGGA GAAACTAAAA GCCCACAGCC AGCGTCTGGT GTCGGTTCAG 
TCCATGCTAA GGCAGACGGG GCGGTACCGA ACTTTGGAGA GGGATCTGAT GGAGTTGCAG 
GAGAGGAAAC TCTTTGAGTA TTTCATAGTC GTTGCGCTTC ACAAAACCAA GGCCGGAGTT 
CCATACCTGC CCGAGGTCAC ACAGCAGTTC CCTCTCAAGC TTGAGAGGAG CTTTAAGTTC 
ATGCGGGAGA CTGAGGACCA GCTGAAGGTC ATCCCTCAGT TCTGTTTCCC TGACGCCAAA 
GACTGGGCAC CTGTCGACAA CTTCCCCAGT GAAACGTTCT CATTCGTCCT GACTGGTGAG 
GATGGAAGCA GGCGGTTCGG CTACTGTCGA CGATTACTGC CCAGTGGTAA AGGCCGAAGG 
CTCCCTGAGG TCTACTGCAT CGTCAGCCGC CTGGGCTGCT TCGACCTCTT CTCCAAGATC 
CTGGATGAAG TGGAAAAGAG GAGGGCTATC TCTCCTGCTC TGGTGCAGCC TTTTATGAGA 
GGGATCATGG AAGCTCCCTT CCCTGCTCCA GGAAGGACCA TCACTGTCAA AAACTTCCTA 
CCTGGCTCTG GGACAGAGGT GATAGAGTTG TGTCGGCCGT CAGATTCCCG TCTGGAGCAT 
GTGGACTTTG AATGTCTCTT CTCCTCCTTG AGTCTGCGTC TCCTTCTGCG AGTGTTTGCC 
TCTCTGCTGC TGGAGCGCAG GGTCATCTTC ACCGCTGACA AACTCAGCAC ATTGTCTCAG 
TGTTGTCATG CAGTCGTGGC TCTTCTCTAC CCCTTCACCT GGCAGCATAC GTACATCCCC 
GTGCTCCCGC CCTCCATGTT GGATATCGTC TGCACGCCTA CACCCTTTAT AGTCGGCCTC 
CTCTCCAGCT CTCTGCCTCG ACTCAAAGAG CTGCCCATCG AAGAAGTCCT GGTGGTCGAC 
CTCGGCAACA GCCGCTTCCT ACGACAGCTG GATGATGAGG ACTCCATTCT TCCTCACAAG 
CTGCAGGCGG CTCTCGAACA CGTGCTGGAC AAGAGGAGGG AGCTGGCATG TGAGAAAGGA 
GATCTGCCCA ATGACTCCAG CTGCCTCAGC ACGGTGGTAT CGGAGGCCTT TGTGCGTTTC 
TTCGTGGAGA TGGTGGGTCA CTACTCCCTC TTTATGGGCG GAGCAGAGCG CGAGGATGAG 
TCTGTGTCCT CCCCCACCTT GCCCAGCCCC TCTTCTGCTG CCTCCTCATC CTTTCAGCGT 
GAGGCCTTTC GCAAAGCGGT CACTTCCAAG AGCTTGAGAA GGTTTCTGGA GGTGTTCATG 
GAGACACAGA TGTTCACCGG CTTCATCCAG GAGAGGGAGC TGCGCAGGCA GGGTCTCAGA 
GGTCTGTTTG AGGTGAGAGC ACAAGAGTAT CTGGATTCGC TGCCTGGAAG CGAACAACGA 
GGAGTCAACA AGTTCCTCAA GGGCCTAGGA AACAAGATGA AATTCCTTTC CAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022611989.1
CDS	431..3610
Translation	MPAASTMTGGRALLLCTNTDNCIYQSVNGLQCCGLKVGEAPSSSVPQPQEVFGSPGNRNGRDTVSSKRPLSPLLSLSDSPGPPDTMLAHRRASNTTDPRTSSMENGLCGKLNHSKSPAGVKSASIRDKISQWEGKKEPAQLTSTGTCPLSTTQKEVETVRKKESKVSEVQRTDCKRFVSWDRQDSGKENVGKLGDSRPKSPEGPTNKDREVILERGFRASKPTEQPLDKKTVLTHVKKLEKATKEVPDRPSLAFPGNYFCPPSKEEVEETEKRAGEPIFGSFDVPRPSGSRRRREGDPENVYSEPGAPSINPLPKPQRTFQHHTPPATPTSGAGSGKGKRNLPPLPSIPPPPLPTCPPPGVCRRPWADKPRDSSNRKSYEFEDLLQSSTESCRVDWYAQSRLGLTRTLSEENVYEDIIDPPSKENPYEDIELERSCLGSKCVSPASSSPVPDTPTKLSSKPGFFRQNSERRSFKLLELRKTGRDTGISSPSRVSPPSTPSSPDDTPCLSGDPYNRRRRKIPKMVLKINGIFEARRGKKRMKRVSQSTESSSGRVTDENSESESDTEEKLKAHSQRLVSVQSMLRQTGRYRTLERDLMELQERKLFEYFIVVALHKTKAGVPYLPEVTQQFPLKLERSFKFMRETEDQLKVIPQFCFPDAKDWAPVDNFPSETFSFVLTGEDGSRRFGYCRRLLPSGKGRRLPEVYCIVSRLGCFDLFSKILDEVEKRRAISPALVQPFMRGIMEAPFPAPGRTITVKNFLPGSGTEVIELCRPSDSRLEHVDFECLFSSLSLRLLLRVFASLLLERRVIFTADKLSTLSQCCHAVVALLYPFTWQHTYIPVLPPSMLDIVCTPTPFIVGLLSSSLPRLKELPIEEVLVVDLGNSRFLRQLDDEDSILPHKLQAALEHVLDKRRELACEKGDLPNDSSCLSTVVSEAFVRFFVEMVGHYSLFMGGAEREDESVSSPTLPSPSSAASSSFQREAFRKAVTSKSLRRFLEVFMETQMFTGFIQERELRRQGLRGLFEVRAQEYLDSLPGSEQRGVNKFLKGLGNKMKFLSKK

Target ORF information:

RefSeq Version	XM_022756268.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili DENN domain containing 2A (dennd2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022756268.1

ORF Insert Sequence:

ATGCCAGCTG CTAGCACCAT GACTGGTGGG AGGGCCCTGC TGCTCTGTAC AAACACTGAC 
AACTGTATCT ACCAATCAGT CAACGGGCTC CAGTGCTGTG GCTTGAAGGT TGGGGAGGCT 
CCCTCATCTT CGGTGCCCCA GCCCCAAGAG GTGTTCGGGT CACCGGGGAA CAGAAACGGG 
AGGGACACTG TGTCTTCCAA GCGTCCCCTC TCACCCTTAC TCAGCCTGAG CGACAGCCCG 
GGTCCTCCTG ACACCATGTT GGCTCACAGG AGAGCCAGCA ACACCACCGA CCCGCGGACA 
TCCAGCATGG AGAACGGACT GTGTGGCAAA CTGAACCACA GCAAGAGCCC AGCAGGAGTC 
AAGAGTGCAA GCATCCGCGA TAAGATCTCA CAATGGGAGG GCAAAAAGGA ACCTGCCCAA 
CTTACCTCTA CAGGGACATG TCCACTGAGC ACGACACAAA AAGAAGTTGA GACAGTGAGG 
AAAAAGGAAT CTAAAGTTTC AGAGGTCCAA AGGACAGATT GCAAGCGGTT TGTCAGCTGG 
GACAGACAAG ACTCAGGGAA GGAGAATGTC GGAAAGCTTG GGGATTCAAG GCCGAAATCT 
CCAGAAGGCC CAACAAACAA AGACAGAGAA GTGATACTAG AGAGAGGGTT TCGGGCTTCT 
AAGCCAACAG AGCAACCCCT CGACAAGAAA ACTGTTTTAA CTCATGTTAA GAAACTGGAG 
AAGGCAACAA AGGAAGTTCC TGACAGACCT TCACTTGCAT TTCCAGGGAA TTACTTCTGT 
CCTCCTTCAA AGGAGGAGGT GGAAGAAACA GAAAAGAGGG CCGGCGAGCC CATTTTTGGG 
AGTTTTGACG TCCCTCGGCC CAGTGGGTCA CGGAGGAGGA GGGAGGGGGA TCCAGAGAAC 
GTATACAGCG AGCCAGGTGC TCCATCTATA AACCCTCTAC CTAAACCTCA GAGAACCTTC 
CAGCACCACA CACCGCCCGC TACCCCAACT TCAGGGGCCG GCTCGGGGAA GGGAAAGAGG 
AACTTGCCCC CTTTGCCCTC CATTCCTCCT CCACCTTTAC CAACATGCCC ACCACCTGGA 
GTGTGCAGGA GACCCTGGGC TGACAAACCC CGGGACAGCA GTAACAGGAA GTCGTATGAA 
TTCGAGGATC TGCTCCAGTC GTCTACAGAG AGCTGCAGGG TGGACTGGTA CGCTCAGTCC 
AGACTGGGCC TAACACGCAC TTTATCAGAA GAGAATGTCT ACGAGGACAT AATAGATCCT 
CCATCCAAGG AGAACCCTTA TGAAGATATA GAGCTGGAGA GAAGTTGTTT GGGAAGCAAA 
TGTGTTTCAC CTGCCTCCTC ATCTCCTGTC CCTGACACGC CAACTAAGCT CTCCTCCAAG 
CCCGGCTTCT TCAGACAAAA CTCAGAACGG CGAAGTTTTA AGCTCCTCGA ACTACGCAAG 
ACCGGCAGGG ACACTGGCAT CTCCTCACCC TCCCGTGTCA GCCCGCCCTC CACACCCAGC 
AGCCCCGACG ACACCCCCTG CCTCTCAGGA GACCCGTACA ATCGCAGACG GAGGAAAATC 
CCCAAGATGG TGCTGAAAAT CAATGGCATC TTTGAGGCAC GAAGAGGGAA GAAACGCATG 
AAGAGAGTGT CTCAGTCGAC CGAGTCCAGC TCAGGGAGAG TGACAGATGA GAACAGTGAG 
TCGGAAAGTG ACACGGAGGA GAAACTAAAA GCCCACAGCC AGCGTCTGGT GTCGGTTCAG 
TCCATGCTAA GGCAGACGGG GCGGTACCGA ACTTTGGAGA GGGATCTGAT GGAGTTGCAG 
GAGAGGAAAC TCTTTGAGTA TTTCATAGTC GTTGCGCTTC ACAAAACCAA GGCCGGAGTT 
CCATACCTGC CCGAGGTCAC ACAGCAGTTC CCTCTCAAGC TTGAGAGGAG CTTTAAGTTC 
ATGCGGGAGA CTGAGGACCA GCTGAAGGTC ATCCCTCAGT TCTGTTTCCC TGACGCCAAA 
GACTGGGCAC CTGTCGACAA CTTCCCCAGT GAAACGTTCT CATTCGTCCT GACTGGTGAG 
GATGGAAGCA GGCGGTTCGG CTACTGTCGA CGATTACTGC CCAGTGGTAA AGGCCGAAGG 
CTCCCTGAGG TCTACTGCAT CGTCAGCCGC CTGGGCTGCT TCGACCTCTT CTCCAAGATC 
CTGGATGAAG TGGAAAAGAG GAGGGCTATC TCTCCTGCTC TGGTGCAGCC TTTTATGAGA 
GGGATCATGG AAGCTCCCTT CCCTGCTCCA GGAAGGACCA TCACTGTCAA AAACTTCCTA 
CCTGGCTCTG GGACAGAGGT GATAGAGTTG TGTCGGCCGT CAGATTCCCG TCTGGAGCAT 
GTGGACTTTG AATGTCTCTT CTCCTCCTTG AGTCTGCGTC TCCTTCTGCG AGTGTTTGCC 
TCTCTGCTGC TGGAGCGCAG GGTCATCTTC ACCGCTGACA AACTCAGCAC ATTGTCTCAG 
TGTTGTCATG CAGTCGTGGC TCTTCTCTAC CCCTTCACCT GGCAGCATAC GTACATCCCC 
GTGCTCCCGC CCTCCATGTT GGATATCGTC TGCACGCCTA CACCCTTTAT AGTCGGCCTC 
CTCTCCAGCT CTCTGCCTCG ACTCAAAGAG CTGCCCATCG AAGAAGTCCT GGTGGTCGAC 
CTCGGCAACA GCCGCTTCCT ACGACAGCTG GATGATGAGG ACTCCATTCT TCCTCACAAG 
CTGCAGGCGG CTCTCGAACA CGTGCTGGAC AAGAGGAGGG AGCTGGCATG TGAGAAAGGA 
GATCTGCCCA ATGACTCCAG CTGCCTCAGC ACGGTGGTAT CGGAGGCCTT TGTGCGTTTC 
TTCGTGGAGA TGGTGGGTCA CTACTCCCTC TTTATGGGCG GAGCAGAGCG CGAGGATGAG 
TCTGTGTCCT CCCCCACCTT GCCCAGCCCC TCTTCTGCTG CCTCCTCATC CTTTCAGCGT 
GAGGCCTTTC GCAAAGCGGT CACTTCCAAG AGCTTGAGAA GGTTTCTGGA GGTGTTCATG 
GAGACACAGA TGTTCACCGG CTTCATCCAG GAGAGGGAGC TGCGCAGGCA GGGTCTCAGA 
GGTCTGTTTG AGGTGAGAGC ACAAGAGTAT CTGGATTCGC TGCCTGGAAG CGAACAACGA 
GGAGTCAACA AGTTCCTCAA GGGCCTAGGA AACAAGATGA AATTCCTTTC CAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd2a ( XM_022756269.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022611990.1 Seriola dumerili DENN domain containing 2A (dennd2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe202592
Clone ID Related Accession (Same CDS sequence)	XM_022756268.1 , XM_022756269.1
Accession Version	XM_022756269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	DENN domain-containing protein 2A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174497.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG CTAGCACCAT GACTGGTGGG AGGGCCCTGC TGCTCTGTAC AAACACTGAC 
AACTGTATCT ACCAATCAGT CAACGGGCTC CAGTGCTGTG GCTTGAAGGT TGGGGAGGCT 
CCCTCATCTT CGGTGCCCCA GCCCCAAGAG GTGTTCGGGT CACCGGGGAA CAGAAACGGG 
AGGGACACTG TGTCTTCCAA GCGTCCCCTC TCACCCTTAC TCAGCCTGAG CGACAGCCCG 
GGTCCTCCTG ACACCATGTT GGCTCACAGG AGAGCCAGCA ACACCACCGA CCCGCGGACA 
TCCAGCATGG AGAACGGACT GTGTGGCAAA CTGAACCACA GCAAGAGCCC AGCAGGAGTC 
AAGAGTGCAA GCATCCGCGA TAAGATCTCA CAATGGGAGG GCAAAAAGGA ACCTGCCCAA 
CTTACCTCTA CAGGGACATG TCCACTGAGC ACGACACAAA AAGAAGTTGA GACAGTGAGG 
AAAAAGGAAT CTAAAGTTTC AGAGGTCCAA AGGACAGATT GCAAGCGGTT TGTCAGCTGG 
GACAGACAAG ACTCAGGGAA GGAGAATGTC GGAAAGCTTG GGGATTCAAG GCCGAAATCT 
CCAGAAGGCC CAACAAACAA AGACAGAGAA GTGATACTAG AGAGAGGGTT TCGGGCTTCT 
AAGCCAACAG AGCAACCCCT CGACAAGAAA ACTGTTTTAA CTCATGTTAA GAAACTGGAG 
AAGGCAACAA AGGAAGTTCC TGACAGACCT TCACTTGCAT TTCCAGGGAA TTACTTCTGT 
CCTCCTTCAA AGGAGGAGGT GGAAGAAACA GAAAAGAGGG CCGGCGAGCC CATTTTTGGG 
AGTTTTGACG TCCCTCGGCC CAGTGGGTCA CGGAGGAGGA GGGAGGGGGA TCCAGAGAAC 
GTATACAGCG AGCCAGGTGC TCCATCTATA AACCCTCTAC CTAAACCTCA GAGAACCTTC 
CAGCACCACA CACCGCCCGC TACCCCAACT TCAGGGGCCG GCTCGGGGAA GGGAAAGAGG 
AACTTGCCCC CTTTGCCCTC CATTCCTCCT CCACCTTTAC CAACATGCCC ACCACCTGGA 
GTGTGCAGGA GACCCTGGGC TGACAAACCC CGGGACAGCA GTAACAGGAA GTCGTATGAA 
TTCGAGGATC TGCTCCAGTC GTCTACAGAG AGCTGCAGGG TGGACTGGTA CGCTCAGTCC 
AGACTGGGCC TAACACGCAC TTTATCAGAA GAGAATGTCT ACGAGGACAT AATAGATCCT 
CCATCCAAGG AGAACCCTTA TGAAGATATA GAGCTGGAGA GAAGTTGTTT GGGAAGCAAA 
TGTGTTTCAC CTGCCTCCTC ATCTCCTGTC CCTGACACGC CAACTAAGCT CTCCTCCAAG 
CCCGGCTTCT TCAGACAAAA CTCAGAACGG CGAAGTTTTA AGCTCCTCGA ACTACGCAAG 
ACCGGCAGGG ACACTGGCAT CTCCTCACCC TCCCGTGTCA GCCCGCCCTC CACACCCAGC 
AGCCCCGACG ACACCCCCTG CCTCTCAGGA GACCCGTACA ATCGCAGACG GAGGAAAATC 
CCCAAGATGG TGCTGAAAAT CAATGGCATC TTTGAGGCAC GAAGAGGGAA GAAACGCATG 
AAGAGAGTGT CTCAGTCGAC CGAGTCCAGC TCAGGGAGAG TGACAGATGA GAACAGTGAG 
TCGGAAAGTG ACACGGAGGA GAAACTAAAA GCCCACAGCC AGCGTCTGGT GTCGGTTCAG 
TCCATGCTAA GGCAGACGGG GCGGTACCGA ACTTTGGAGA GGGATCTGAT GGAGTTGCAG 
GAGAGGAAAC TCTTTGAGTA TTTCATAGTC GTTGCGCTTC ACAAAACCAA GGCCGGAGTT 
CCATACCTGC CCGAGGTCAC ACAGCAGTTC CCTCTCAAGC TTGAGAGGAG CTTTAAGTTC 
ATGCGGGAGA CTGAGGACCA GCTGAAGGTC ATCCCTCAGT TCTGTTTCCC TGACGCCAAA 
GACTGGGCAC CTGTCGACAA CTTCCCCAGT GAAACGTTCT CATTCGTCCT GACTGGTGAG 
GATGGAAGCA GGCGGTTCGG CTACTGTCGA CGATTACTGC CCAGTGGTAA AGGCCGAAGG 
CTCCCTGAGG TCTACTGCAT CGTCAGCCGC CTGGGCTGCT TCGACCTCTT CTCCAAGATC 
CTGGATGAAG TGGAAAAGAG GAGGGCTATC TCTCCTGCTC TGGTGCAGCC TTTTATGAGA 
GGGATCATGG AAGCTCCCTT CCCTGCTCCA GGAAGGACCA TCACTGTCAA AAACTTCCTA 
CCTGGCTCTG GGACAGAGGT GATAGAGTTG TGTCGGCCGT CAGATTCCCG TCTGGAGCAT 
GTGGACTTTG AATGTCTCTT CTCCTCCTTG AGTCTGCGTC TCCTTCTGCG AGTGTTTGCC 
TCTCTGCTGC TGGAGCGCAG GGTCATCTTC ACCGCTGACA AACTCAGCAC ATTGTCTCAG 
TGTTGTCATG CAGTCGTGGC TCTTCTCTAC CCCTTCACCT GGCAGCATAC GTACATCCCC 
GTGCTCCCGC CCTCCATGTT GGATATCGTC TGCACGCCTA CACCCTTTAT AGTCGGCCTC 
CTCTCCAGCT CTCTGCCTCG ACTCAAAGAG CTGCCCATCG AAGAAGTCCT GGTGGTCGAC 
CTCGGCAACA GCCGCTTCCT ACGACAGCTG GATGATGAGG ACTCCATTCT TCCTCACAAG 
CTGCAGGCGG CTCTCGAACA CGTGCTGGAC AAGAGGAGGG AGCTGGCATG TGAGAAAGGA 
GATCTGCCCA ATGACTCCAG CTGCCTCAGC ACGGTGGTAT CGGAGGCCTT TGTGCGTTTC 
TTCGTGGAGA TGGTGGGTCA CTACTCCCTC TTTATGGGCG GAGCAGAGCG CGAGGATGAG 
TCTGTGTCCT CCCCCACCTT GCCCAGCCCC TCTTCTGCTG CCTCCTCATC CTTTCAGCGT 
GAGGCCTTTC GCAAAGCGGT CACTTCCAAG AGCTTGAGAA GGTTTCTGGA GGTGTTCATG 
GAGACACAGA TGTTCACCGG CTTCATCCAG GAGAGGGAGC TGCGCAGGCA GGGTCTCAGA 
GGTCTGTTTG AGGTGAGAGC ACAAGAGTAT CTGGATTCGC TGCCTGGAAG CGAACAACGA 
GGAGTCAACA AGTTCCTCAA GGGCCTAGGA AACAAGATGA AATTCCTTTC CAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022611990.1
CDS	383..3562
Translation	MPAASTMTGGRALLLCTNTDNCIYQSVNGLQCCGLKVGEAPSSSVPQPQEVFGSPGNRNGRDTVSSKRPLSPLLSLSDSPGPPDTMLAHRRASNTTDPRTSSMENGLCGKLNHSKSPAGVKSASIRDKISQWEGKKEPAQLTSTGTCPLSTTQKEVETVRKKESKVSEVQRTDCKRFVSWDRQDSGKENVGKLGDSRPKSPEGPTNKDREVILERGFRASKPTEQPLDKKTVLTHVKKLEKATKEVPDRPSLAFPGNYFCPPSKEEVEETEKRAGEPIFGSFDVPRPSGSRRRREGDPENVYSEPGAPSINPLPKPQRTFQHHTPPATPTSGAGSGKGKRNLPPLPSIPPPPLPTCPPPGVCRRPWADKPRDSSNRKSYEFEDLLQSSTESCRVDWYAQSRLGLTRTLSEENVYEDIIDPPSKENPYEDIELERSCLGSKCVSPASSSPVPDTPTKLSSKPGFFRQNSERRSFKLLELRKTGRDTGISSPSRVSPPSTPSSPDDTPCLSGDPYNRRRRKIPKMVLKINGIFEARRGKKRMKRVSQSTESSSGRVTDENSESESDTEEKLKAHSQRLVSVQSMLRQTGRYRTLERDLMELQERKLFEYFIVVALHKTKAGVPYLPEVTQQFPLKLERSFKFMRETEDQLKVIPQFCFPDAKDWAPVDNFPSETFSFVLTGEDGSRRFGYCRRLLPSGKGRRLPEVYCIVSRLGCFDLFSKILDEVEKRRAISPALVQPFMRGIMEAPFPAPGRTITVKNFLPGSGTEVIELCRPSDSRLEHVDFECLFSSLSLRLLLRVFASLLLERRVIFTADKLSTLSQCCHAVVALLYPFTWQHTYIPVLPPSMLDIVCTPTPFIVGLLSSSLPRLKELPIEEVLVVDLGNSRFLRQLDDEDSILPHKLQAALEHVLDKRRELACEKGDLPNDSSCLSTVVSEAFVRFFVEMVGHYSLFMGGAEREDESVSSPTLPSPSSAASSSFQREAFRKAVTSKSLRRFLEVFMETQMFTGFIQERELRRQGLRGLFEVRAQEYLDSLPGSEQRGVNKFLKGLGNKMKFLSKK

Target ORF information:

RefSeq Version	XM_022756269.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili DENN domain containing 2A (dennd2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022756269.1

ORF Insert Sequence:

ATGCCAGCTG CTAGCACCAT GACTGGTGGG AGGGCCCTGC TGCTCTGTAC AAACACTGAC 
AACTGTATCT ACCAATCAGT CAACGGGCTC CAGTGCTGTG GCTTGAAGGT TGGGGAGGCT 
CCCTCATCTT CGGTGCCCCA GCCCCAAGAG GTGTTCGGGT CACCGGGGAA CAGAAACGGG 
AGGGACACTG TGTCTTCCAA GCGTCCCCTC TCACCCTTAC TCAGCCTGAG CGACAGCCCG 
GGTCCTCCTG ACACCATGTT GGCTCACAGG AGAGCCAGCA ACACCACCGA CCCGCGGACA 
TCCAGCATGG AGAACGGACT GTGTGGCAAA CTGAACCACA GCAAGAGCCC AGCAGGAGTC 
AAGAGTGCAA GCATCCGCGA TAAGATCTCA CAATGGGAGG GCAAAAAGGA ACCTGCCCAA 
CTTACCTCTA CAGGGACATG TCCACTGAGC ACGACACAAA AAGAAGTTGA GACAGTGAGG 
AAAAAGGAAT CTAAAGTTTC AGAGGTCCAA AGGACAGATT GCAAGCGGTT TGTCAGCTGG 
GACAGACAAG ACTCAGGGAA GGAGAATGTC GGAAAGCTTG GGGATTCAAG GCCGAAATCT 
CCAGAAGGCC CAACAAACAA AGACAGAGAA GTGATACTAG AGAGAGGGTT TCGGGCTTCT 
AAGCCAACAG AGCAACCCCT CGACAAGAAA ACTGTTTTAA CTCATGTTAA GAAACTGGAG 
AAGGCAACAA AGGAAGTTCC TGACAGACCT TCACTTGCAT TTCCAGGGAA TTACTTCTGT 
CCTCCTTCAA AGGAGGAGGT GGAAGAAACA GAAAAGAGGG CCGGCGAGCC CATTTTTGGG 
AGTTTTGACG TCCCTCGGCC CAGTGGGTCA CGGAGGAGGA GGGAGGGGGA TCCAGAGAAC 
GTATACAGCG AGCCAGGTGC TCCATCTATA AACCCTCTAC CTAAACCTCA GAGAACCTTC 
CAGCACCACA CACCGCCCGC TACCCCAACT TCAGGGGCCG GCTCGGGGAA GGGAAAGAGG 
AACTTGCCCC CTTTGCCCTC CATTCCTCCT CCACCTTTAC CAACATGCCC ACCACCTGGA 
GTGTGCAGGA GACCCTGGGC TGACAAACCC CGGGACAGCA GTAACAGGAA GTCGTATGAA 
TTCGAGGATC TGCTCCAGTC GTCTACAGAG AGCTGCAGGG TGGACTGGTA CGCTCAGTCC 
AGACTGGGCC TAACACGCAC TTTATCAGAA GAGAATGTCT ACGAGGACAT AATAGATCCT 
CCATCCAAGG AGAACCCTTA TGAAGATATA GAGCTGGAGA GAAGTTGTTT GGGAAGCAAA 
TGTGTTTCAC CTGCCTCCTC ATCTCCTGTC CCTGACACGC CAACTAAGCT CTCCTCCAAG 
CCCGGCTTCT TCAGACAAAA CTCAGAACGG CGAAGTTTTA AGCTCCTCGA ACTACGCAAG 
ACCGGCAGGG ACACTGGCAT CTCCTCACCC TCCCGTGTCA GCCCGCCCTC CACACCCAGC 
AGCCCCGACG ACACCCCCTG CCTCTCAGGA GACCCGTACA ATCGCAGACG GAGGAAAATC 
CCCAAGATGG TGCTGAAAAT CAATGGCATC TTTGAGGCAC GAAGAGGGAA GAAACGCATG 
AAGAGAGTGT CTCAGTCGAC CGAGTCCAGC TCAGGGAGAG TGACAGATGA GAACAGTGAG 
TCGGAAAGTG ACACGGAGGA GAAACTAAAA GCCCACAGCC AGCGTCTGGT GTCGGTTCAG 
TCCATGCTAA GGCAGACGGG GCGGTACCGA ACTTTGGAGA GGGATCTGAT GGAGTTGCAG 
GAGAGGAAAC TCTTTGAGTA TTTCATAGTC GTTGCGCTTC ACAAAACCAA GGCCGGAGTT 
CCATACCTGC CCGAGGTCAC ACAGCAGTTC CCTCTCAAGC TTGAGAGGAG CTTTAAGTTC 
ATGCGGGAGA CTGAGGACCA GCTGAAGGTC ATCCCTCAGT TCTGTTTCCC TGACGCCAAA 
GACTGGGCAC CTGTCGACAA CTTCCCCAGT GAAACGTTCT CATTCGTCCT GACTGGTGAG 
GATGGAAGCA GGCGGTTCGG CTACTGTCGA CGATTACTGC CCAGTGGTAA AGGCCGAAGG 
CTCCCTGAGG TCTACTGCAT CGTCAGCCGC CTGGGCTGCT TCGACCTCTT CTCCAAGATC 
CTGGATGAAG TGGAAAAGAG GAGGGCTATC TCTCCTGCTC TGGTGCAGCC TTTTATGAGA 
GGGATCATGG AAGCTCCCTT CCCTGCTCCA GGAAGGACCA TCACTGTCAA AAACTTCCTA 
CCTGGCTCTG GGACAGAGGT GATAGAGTTG TGTCGGCCGT CAGATTCCCG TCTGGAGCAT 
GTGGACTTTG AATGTCTCTT CTCCTCCTTG AGTCTGCGTC TCCTTCTGCG AGTGTTTGCC 
TCTCTGCTGC TGGAGCGCAG GGTCATCTTC ACCGCTGACA AACTCAGCAC ATTGTCTCAG 
TGTTGTCATG CAGTCGTGGC TCTTCTCTAC CCCTTCACCT GGCAGCATAC GTACATCCCC 
GTGCTCCCGC CCTCCATGTT GGATATCGTC TGCACGCCTA CACCCTTTAT AGTCGGCCTC 
CTCTCCAGCT CTCTGCCTCG ACTCAAAGAG CTGCCCATCG AAGAAGTCCT GGTGGTCGAC 
CTCGGCAACA GCCGCTTCCT ACGACAGCTG GATGATGAGG ACTCCATTCT TCCTCACAAG 
CTGCAGGCGG CTCTCGAACA CGTGCTGGAC AAGAGGAGGG AGCTGGCATG TGAGAAAGGA 
GATCTGCCCA ATGACTCCAG CTGCCTCAGC ACGGTGGTAT CGGAGGCCTT TGTGCGTTTC 
TTCGTGGAGA TGGTGGGTCA CTACTCCCTC TTTATGGGCG GAGCAGAGCG CGAGGATGAG 
TCTGTGTCCT CCCCCACCTT GCCCAGCCCC TCTTCTGCTG CCTCCTCATC CTTTCAGCGT 
GAGGCCTTTC GCAAAGCGGT CACTTCCAAG AGCTTGAGAA GGTTTCTGGA GGTGTTCATG 
GAGACACAGA TGTTCACCGG CTTCATCCAG GAGAGGGAGC TGCGCAGGCA GGGTCTCAGA 
GGTCTGTTTG AGGTGAGAGC ACAAGAGTAT CTGGATTCGC TGCCTGGAAG CGAACAACGA 
GGAGTCAACA AGTTCCTCAA GGGCCTAGGA AACAAGATGA AATTCCTTTC CAAGAAATGA