chrnb1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	chrnb1
Entrez Gene ID	111226935
Full Name	cholinergic receptor nicotinic beta 1 subunit
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022752251.1	XP_022607972.1	acetylcholine receptor subunit beta isoform X2
XM_022752250.1	XP_022607971.1	acetylcholine receptor subunit beta isoform X1

Pathways from BioSystems
Gene Ontology

The following chrnb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chrnb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe199326	XM_022752250.1 Latest version!	Seriola dumerili cholinergic receptor nicotinic beta 1 subunit (chrnb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OSe199327	XM_022752251.1 Latest version!	Seriola dumerili cholinergic receptor nicotinic beta 1 subunit (chrnb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chrnb1 ( XM_022752250.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022607971.1 Seriola dumerili cholinergic receptor nicotinic beta 1 subunit (chrnb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe199326
Clone ID Related Accession (Same CDS sequence)	XM_022752250.1
Accession Version	XM_022752250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	acetylcholine receptor subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174422.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC TGGATCTGCT CCTGCTGGCA TGTTGCTTAT GCAGCCTGAG CACGTTGGGA 
GGTGCATCTG AAATGGAGCA GATTATGATG AAGAAGCTCT TCGCCAACTA CAACGTAAAG 
GTCCGGCCAG CACCCAGCCC TGAAGAGAGG GTGGTGGTCC GTGTGGGAAT GATCCTCTCT 
TCCTTCGTTG GACTGAATAT GAAAAATGAA GAGATGAGCA CTGTGGTTGT CATGAATCTG 
GAATGGACAG ATCATCGGTT GACATGGAAA CCCAAGCAGC ATGATGGGAT TGAAGTGCTG 
CGTATCCCCG CTGCGAGGGT TTGGCTGCCT GACATTGTCC TCATCAATAA TAATGATGGG 
GTGTTTGATG TGGCCTTGCA TGTCCATGTG CAGGTTTATA GTAACGGCAG AGTAACCTGG 
ACTCCCCCTG CACTATACTG CAGCTCCTGT GGAGTTAAGG TAACATACTT CCCATTCGAC 
TGGCAGAACT GCACCATGCA GTTCCGCTCC TACACGTATG ACTCGACAGA GATCGACCTG 
CAGTATGCAC TTGACTCAAA GGGCAACGAG ATACGGGAGA TCCAACTGGA CGAGGCTTAC 
AGCGAGGGCG GCGAGTGGCG CATCATGCAC AAGCCGAGCA GGAAGAGCAC GAATGATGAT 
CTGTACGAGG ACATGATCTT CTACCTGATC ATTGAGAGGA AGCCTCTGTA CTACGTCCTG 
AACATCATCC TCCCCTGTAT TCTCATCACC ATCATCGCCA TCTTTAACTT CTACCTGCCT 
CCTGATGCAG GTACACAGAG TGAAAAGATG GGTCTGTCCA TCAACGTGCT GCTGACCCTC 
ACTGTGTTCC TGCTGCTGCT GGCTGATAAG ATCCCTGAGA CGTCACTGGG CGTCCCCATC 
ATCGTCAACT ACATCATGTT CACCATGATC CTGGTCACCT TCTCCGTCAT CCTCAGCGTG 
GTCGTCCTTA ACCTGCATCA CCGCTCGCCC AACACCCACC TGATGCCCAT GTGGGTCCGC 
AAGATCTTCA TCCACATGCT ACCTCCTTAC CTTGGCATGC TACGGCCAAA AGTGGAGACC 
CCCCTGTCGC TGGAGACCCT GCCCCGTCGG GAGAAAAAGA AGATGGCCAT CAGCAAAGTG 
GCTGATGAAT ACTTTATCCG CAAACCGGAC TCCTCCATCT TGTTCCCCAA GCCTAACAGG 
TTTCAGCCAG AGGGCATGTG TACGGACCTG AGGAAATTTA TCGACGGCCC CAGCAGCTAC 
CTATCGTTAC CTGAAGAGCT GAAGTCGGCC ATAGACGCCA TCACTTATAT CGCAGAGGCT 
CTGCAGGCTG AGAAGGACTA TGAAGCTCTG AGAGAGGACT GGCAGTACGT GGCTATGGTG 
GTGGACCGCA TGTTCCTCTG GATCTTCGTC GTCTTCACCA CCGTGGGAAC CTTAGCCATC 
TTCGCCGATG CCAGCTTCAA CCACACTCCC ACCGACCCCT TCAAGTCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022607971.1
CDS	171..1664
Translation	MKSLDLLLLACCLCSLSTLGGASEMEQIMMKKLFANYNVKVRPAPSPEERVVVRVGMILSSFVGLNMKNEEMSTVVVMNLEWTDHRLTWKPKQHDGIEVLRIPAARVWLPDIVLINNNDGVFDVALHVHVQVYSNGRVTWTPPALYCSSCGVKVTYFPFDWQNCTMQFRSYTYDSTEIDLQYALDSKGNEIREIQLDEAYSEGGEWRIMHKPSRKSTNDDLYEDMIFYLIIERKPLYYVLNIILPCILITIIAIFNFYLPPDAGTQSEKMGLSINVLLTLTVFLLLLADKIPETSLGVPIIVNYIMFTMILVTFSVILSVVVLNLHHRSPNTHLMPMWVRKIFIHMLPPYLGMLRPKVETPLSLETLPRREKKKMAISKVADEYFIRKPDSSILFPKPNRFQPEGMCTDLRKFIDGPSSYLSLPEELKSAIDAITYIAEALQAEKDYEALREDWQYVAMVVDRMFLWIFVVFTTVGTLAIFADASFNHTPTDPFKSP

Target ORF information:

RefSeq Version	XM_022752250.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili cholinergic receptor nicotinic beta 1 subunit (chrnb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022752250.1

ORF Insert Sequence:

ATGAAAAGCC TGGATCTGCT CCTGCTGGCA TGTTGCTTAT GCAGCCTGAG CACGTTGGGA 
GGTGCATCTG AAATGGAGCA GATTATGATG AAGAAGCTCT TCGCCAACTA CAACGTAAAG 
GTCCGGCCAG CACCCAGCCC TGAAGAGAGG GTGGTGGTCC GTGTGGGAAT GATCCTCTCT 
TCCTTCGTTG GACTGAATAT GAAAAATGAA GAGATGAGCA CTGTGGTTGT CATGAATCTG 
GAATGGACAG ATCATCGGTT GACATGGAAA CCCAAGCAGC ATGATGGGAT TGAAGTGCTG 
CGTATCCCCG CTGCGAGGGT TTGGCTGCCT GACATTGTCC TCATCAATAA TAATGATGGG 
GTGTTTGATG TGGCCTTGCA TGTCCATGTG CAGGTTTATA GTAACGGCAG AGTAACCTGG 
ACTCCCCCTG CACTATACTG CAGCTCCTGT GGAGTTAAGG TAACATACTT CCCATTCGAC 
TGGCAGAACT GCACCATGCA GTTCCGCTCC TACACGTATG ACTCGACAGA GATCGACCTG 
CAGTATGCAC TTGACTCAAA GGGCAACGAG ATACGGGAGA TCCAACTGGA CGAGGCTTAC 
AGCGAGGGCG GCGAGTGGCG CATCATGCAC AAGCCGAGCA GGAAGAGCAC GAATGATGAT 
CTGTACGAGG ACATGATCTT CTACCTGATC ATTGAGAGGA AGCCTCTGTA CTACGTCCTG 
AACATCATCC TCCCCTGTAT TCTCATCACC ATCATCGCCA TCTTTAACTT CTACCTGCCT 
CCTGATGCAG GTACACAGAG TGAAAAGATG GGTCTGTCCA TCAACGTGCT GCTGACCCTC 
ACTGTGTTCC TGCTGCTGCT GGCTGATAAG ATCCCTGAGA CGTCACTGGG CGTCCCCATC 
ATCGTCAACT ACATCATGTT CACCATGATC CTGGTCACCT TCTCCGTCAT CCTCAGCGTG 
GTCGTCCTTA ACCTGCATCA CCGCTCGCCC AACACCCACC TGATGCCCAT GTGGGTCCGC 
AAGATCTTCA TCCACATGCT ACCTCCTTAC CTTGGCATGC TACGGCCAAA AGTGGAGACC 
CCCCTGTCGC TGGAGACCCT GCCCCGTCGG GAGAAAAAGA AGATGGCCAT CAGCAAAGTG 
GCTGATGAAT ACTTTATCCG CAAACCGGAC TCCTCCATCT TGTTCCCCAA GCCTAACAGG 
TTTCAGCCAG AGGGCATGTG TACGGACCTG AGGAAATTTA TCGACGGCCC CAGCAGCTAC 
CTATCGTTAC CTGAAGAGCT GAAGTCGGCC ATAGACGCCA TCACTTATAT CGCAGAGGCT 
CTGCAGGCTG AGAAGGACTA TGAAGCTCTG AGAGAGGACT GGCAGTACGT GGCTATGGTG 
GTGGACCGCA TGTTCCTCTG GATCTTCGTC GTCTTCACCA CCGTGGGAAC CTTAGCCATC 
TTCGCCGATG CCAGCTTCAA CCACACTCCC ACCGACCCCT TCAAGTCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chrnb1 ( XM_022752251.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022607972.1 Seriola dumerili cholinergic receptor nicotinic beta 1 subunit (chrnb1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe199327
Clone ID Related Accession (Same CDS sequence)	XM_022752251.1
Accession Version	XM_022752251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	acetylcholine receptor subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174422.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC TGGATCTGCT CCTGCTGGCA TGTTGCTTAT GCAGCCTGAG CACGTTGGGA 
GGTGCATCTG AAATGGAGCA GATTATGATG AAGAAGCTCT TCGCCAACTA CAACGTAAAG 
GTCCGGCCAG CACCCAGCCC TGAAGAGAGG GTGGTGGTCC GTGTGGGAAT GATCCTCTCT 
TCCTTCGTTG GACTGAATAT GAAAAATGAA GAGATGAGCA CTGTGGTTGT CATGAATCTG 
GAATGGACAG ATCATCGGTT GACATGGAAA CCCAAGCAGC ATGATGGGAT TGAAGTGCTG 
CGTATCCCCG CTGCGAGGGT TTGGCTGCCT GACATTGTCC TCATCAATAA TAATGATGGG 
GTGTTTGATG TGGCCTTGCA TGTCCATGTG CAGGTTTATA GTAACGGCAG AGTAACCTGG 
ACTCCCCCTG CACTATACTG CAGCTCCTGT GGAGTTAAGG TAACATACTT CCCATTCGAC 
TGGCAGAACT GCACCATGCA GTTCCGCTCC TACACGTATG ACTCGACAGA GATCGACCTG 
CAGTATGCAC TTGACTCAAA GGGCAACGAG ATACGGGAGA TCCAACTGGA CGAGGCTTAC 
AGCGAGGGCG GCGAGTGGCG CATCATGCAC AAGCCGAGCA GGAAGAGCAC GAATGATGAT 
CTGTACGAGG ACATGATCTT CTACCTGATC ATTGAGAGGA AGCCTCTGTA CTACGTCCTG 
AACATCATCC TCCCCTGTAT TCTCATCACC ATCATCGCCA TCTTTAACTT CTACCTGCCT 
CCTGATGCAG GTGAAAAGAT GGGTCTGTCC ATCAACGTGC TGCTGACCCT CACTGTGTTC 
CTGCTGCTGC TGGCTGATAA GATCCCTGAG ACGTCACTGG GCGTCCCCAT CATCGTCAAC 
TACATCATGT TCACCATGAT CCTGGTCACC TTCTCCGTCA TCCTCAGCGT GGTCGTCCTT 
AACCTGCATC ACCGCTCGCC CAACACCCAC CTGATGCCCA TGTGGGTCCG CAAGATCTTC 
ATCCACATGC TACCTCCTTA CCTTGGCATG CTACGGCCAA AAGTGGAGAC CCCCCTGTCG 
CTGGAGACCC TGCCCCGTCG GGAGAAAAAG AAGATGGCCA TCAGCAAAGT GGCTGATGAA 
TACTTTATCC GCAAACCGGA CTCCTCCATC TTGTTCCCCA AGCCTAACAG GTTTCAGCCA 
GAGGGCATGT GTACGGACCT GAGGAAATTT ATCGACGGCC CCAGCAGCTA CCTATCGTTA 
CCTGAAGAGC TGAAGTCGGC CATAGACGCC ATCACTTATA TCGCAGAGGC TCTGCAGGCT 
GAGAAGGACT ATGAAGCTCT GAGAGAGGAC TGGCAGTACG TGGCTATGGT GGTGGACCGC 
ATGTTCCTCT GGATCTTCGT CGTCTTCACC ACCGTGGGAA CCTTAGCCAT CTTCGCCGAT 
GCCAGCTTCA ACCACACTCC CACCGACCCC TTCAAGTCCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022607972.1
CDS	171..1655
Translation	MKSLDLLLLACCLCSLSTLGGASEMEQIMMKKLFANYNVKVRPAPSPEERVVVRVGMILSSFVGLNMKNEEMSTVVVMNLEWTDHRLTWKPKQHDGIEVLRIPAARVWLPDIVLINNNDGVFDVALHVHVQVYSNGRVTWTPPALYCSSCGVKVTYFPFDWQNCTMQFRSYTYDSTEIDLQYALDSKGNEIREIQLDEAYSEGGEWRIMHKPSRKSTNDDLYEDMIFYLIIERKPLYYVLNIILPCILITIIAIFNFYLPPDAGEKMGLSINVLLTLTVFLLLLADKIPETSLGVPIIVNYIMFTMILVTFSVILSVVVLNLHHRSPNTHLMPMWVRKIFIHMLPPYLGMLRPKVETPLSLETLPRREKKKMAISKVADEYFIRKPDSSILFPKPNRFQPEGMCTDLRKFIDGPSSYLSLPEELKSAIDAITYIAEALQAEKDYEALREDWQYVAMVVDRMFLWIFVVFTTVGTLAIFADASFNHTPTDPFKSP

Target ORF information:

RefSeq Version	XM_022752251.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili cholinergic receptor nicotinic beta 1 subunit (chrnb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022752251.1

ORF Insert Sequence:

ATGAAAAGCC TGGATCTGCT CCTGCTGGCA TGTTGCTTAT GCAGCCTGAG CACGTTGGGA 
GGTGCATCTG AAATGGAGCA GATTATGATG AAGAAGCTCT TCGCCAACTA CAACGTAAAG 
GTCCGGCCAG CACCCAGCCC TGAAGAGAGG GTGGTGGTCC GTGTGGGAAT GATCCTCTCT 
TCCTTCGTTG GACTGAATAT GAAAAATGAA GAGATGAGCA CTGTGGTTGT CATGAATCTG 
GAATGGACAG ATCATCGGTT GACATGGAAA CCCAAGCAGC ATGATGGGAT TGAAGTGCTG 
CGTATCCCCG CTGCGAGGGT TTGGCTGCCT GACATTGTCC TCATCAATAA TAATGATGGG 
GTGTTTGATG TGGCCTTGCA TGTCCATGTG CAGGTTTATA GTAACGGCAG AGTAACCTGG 
ACTCCCCCTG CACTATACTG CAGCTCCTGT GGAGTTAAGG TAACATACTT CCCATTCGAC 
TGGCAGAACT GCACCATGCA GTTCCGCTCC TACACGTATG ACTCGACAGA GATCGACCTG 
CAGTATGCAC TTGACTCAAA GGGCAACGAG ATACGGGAGA TCCAACTGGA CGAGGCTTAC 
AGCGAGGGCG GCGAGTGGCG CATCATGCAC AAGCCGAGCA GGAAGAGCAC GAATGATGAT 
CTGTACGAGG ACATGATCTT CTACCTGATC ATTGAGAGGA AGCCTCTGTA CTACGTCCTG 
AACATCATCC TCCCCTGTAT TCTCATCACC ATCATCGCCA TCTTTAACTT CTACCTGCCT 
CCTGATGCAG GTGAAAAGAT GGGTCTGTCC ATCAACGTGC TGCTGACCCT CACTGTGTTC 
CTGCTGCTGC TGGCTGATAA GATCCCTGAG ACGTCACTGG GCGTCCCCAT CATCGTCAAC 
TACATCATGT TCACCATGAT CCTGGTCACC TTCTCCGTCA TCCTCAGCGT GGTCGTCCTT 
AACCTGCATC ACCGCTCGCC CAACACCCAC CTGATGCCCA TGTGGGTCCG CAAGATCTTC 
ATCCACATGC TACCTCCTTA CCTTGGCATG CTACGGCCAA AAGTGGAGAC CCCCCTGTCG 
CTGGAGACCC TGCCCCGTCG GGAGAAAAAG AAGATGGCCA TCAGCAAAGT GGCTGATGAA 
TACTTTATCC GCAAACCGGA CTCCTCCATC TTGTTCCCCA AGCCTAACAG GTTTCAGCCA 
GAGGGCATGT GTACGGACCT GAGGAAATTT ATCGACGGCC CCAGCAGCTA CCTATCGTTA 
CCTGAAGAGC TGAAGTCGGC CATAGACGCC ATCACTTATA TCGCAGAGGC TCTGCAGGCT 
GAGAAGGACT ATGAAGCTCT GAGAGAGGAC TGGCAGTACG TGGCTATGGT GGTGGACCGC 
ATGTTCCTCT GGATCTTCGT CGTCTTCACC ACCGTGGGAA CCTTAGCCAT CTTCGCCGAT 
GCCAGCTTCA ACCACACTCC CACCGACCCC TTCAAGTCCC CCTGA