irs2 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	irs2
Entrez Gene ID	111218308
Full Name	insulin receptor substrate 2
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022740557.1	XP_022596278.1	insulin receptor substrate 2

Pathways from BioSystems
Gene Ontology

The following irs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe211555	XM_022740557.1 Latest version!	Seriola dumerili insulin receptor substrate 2 (irs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

irs2 ( XM_022740557.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022596278.1 Seriola dumerili insulin receptor substrate 2 (irs2), mRNA.

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CloneID	OSe211555
Clone ID Related Accession (Same CDS sequence)	XM_022740557.1
Accession Version	XM_022740557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	insulin receptor substrate 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174307.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTC CGCCAAACAC GGGAGACCAG TCGTTAATAC CCAGCAATAC GAACGCGAAC 
AACGCTGTCA AGAAATGCGG ATACCTAAAG AAGCAGAAAC ACGGACACAA GCGCTTTTTT 
GTTTTGAAAG AGCCGAGCGA AGGGTTCCCT GCGCGGCTGG AGTACTACGA GAGCGAGAAG 
AAATGGAAAA ACAAATCGGC CGCGAAGAGA GTTATTCCCC TCGACTGCTG CCTCAATATC 
AACAAGAGAG CGGACGCAAA ACACAAATAT CTTATTGCTC TTTATACCAA GGACGAATAT 
TTTGCCGTAG CGGCAGAAAA CGAGCAGGAA CAAGAGAGCT GGTACCGGGT GCTGACGGAT 
TTAATGGCAG AGGGGAAAGT GTATGATGGC TCGGCGTCTA ACTCAGCGTC CTCGCTGGTT 
GGCTTCGACG AGGCGAGCTA CGGTGTAATA ACGCCGGTTA CCGCCGTCTA CAAGGAGGTA 
TGGCAAGTTA ATCTGAAATC TAAAGGCCTG GGGCAGAGCA GAAATTTGAC CGGGGTGTAC 
AGACTGTGTC TCTCCAGCCG GACTATCAGC TTCGTCAAGT TGAACTCCGA GGTAGCCTCC 
GTCATCTTAC AGCTGATGAA TATCCGGAGG TGCGGCCACT CTGACAGCTT CTTTTTCATT 
GAGGTGGGTC GTTCTGCAGC CACGGGTCCC GGGGAGTTAT GGATGCAGGC TGATGACTCT 
GTGGTGGCAC AAAACATCCA CGAAACTATC TTGGAGGCGA TGAAAGCCAT GAAGGAGCTG 
TCGGAATTCC GTCCGCGCAG CAAGAGCCAG TCGTCTGGGA CAAACCCAAT CTCTGTGCCC 
ACACGGAGGC ACCTGAACAA TCTCCCCCCC AGCCAGACCG GGCTTCCTCG GCGGTCGCGC 
ACTGACAGCA TGGCTGCGAC CTCTCCTGTG AGCAAATTTT CTTCCTGTCG AATACGCACG 
GCAAGCGAGG GCGATGGCAC CATGACACGC CCCATGTCAG TGAGTGGGAG CCCGCTTAGC 
CCGGGGGTCC ACAGGACCCT CCTGAGCAGA TCTCACACCA TCACAGCTCG CCCTTGTCGG 
ACATTTGAAT CCTCTTCCCT CCAGCACAGT AAGTCCATGT CTATGCCCCT GTCTCCCTCC 
CCACCCATGG CCACCCCTAG CCTAGTGAGT CTTTCCTCCT GCCCAGACAG CAGTGCCCCT 
CGCCCCTCCA GCTGCAGTGC CTCCTTCTCA GGCTCACCCA GTGATGCTGG TTTTATATCA 
TGTGAGGAGT ATGGCTCTAG TCCTGCAGAT GCACGGGACC TCAGGCTGCC ACTCACCCTC 
CGTAGCAACA CTCCCGAGTC CCTCAAAGCT GACACCCCCC CCTCCCGGGA CAGCAGTGAG 
CTTGAAGGCT ACATGGTGAT GGAGCGACAG AACCAGAATG GTTACCGTCG GTTACCGGAC 
CTGGACAAGG CATATCGAAA GCGCACATAC TCCCTCACCA CTCCCCGCCA GCAAAGGCCC 
CCTCCCCAAG TATCTTCAGC CTCTCTGGAT GAGTACACTC TTATGAGGGC CACGTACACC 
AGCGGAAGCC AGTCTTCTCG CAGGTGCCAC ACTGCCTCCC CTAAAGGGAC CTATCCTGAG 
GATTACAGGG ATGTCCATAT CGGATCTAAC AGTGTCCAAG CTGACAGTGG TTATATGCCC 
ATGATGCCAG GTGTGACACC TCAGACGCCA GGGTCTAAAG ATGACCCCTA CATGCCCATG 
AGCCCCATGT GTGTTTCTGC TCCAAAGCAG ATCATCAACC CCCATTCGCA CTCCTCTTCA 
GCAGGGCTGG CCCTACGCAC AGACTCCCCC GGGAGTGTTT CTCTGGAGGA GAGTGGCTAC 
ATGCGCATGT GGTGCGGAAC CAAGATGTCA GTGGAGAGCA CAGATGGAAA GCTCACCAAC 
GGAGAGTACC TGAATATGTC TCCTGTTGAC CCTCTAGTGT CTCTCACACC CCCAGACTAC 
ATCCTTAGTC CCTCAGGAGG AGATCCCCAC CCCCAACAAC ATCACAGACA GCCTTATTTT 
TATAGCTCTC TTCCACGCTC ACTTAAAGGC CCATTGCAGC GCAACGGGTC CACAGACACA 
GACCAGTACG TGGTGATGAA TTTACAGAGA CAGCGGATAG AAGAGGAGTC CAACTATTGT 
CCTGTCTCTC CACGAGACTC GGTGGCCAGC AGCTCTCCGG TGACCTCCTC TGCCCCATCT 
CCTCTCAGAC TGGCTCGGTT AGATGGAGGT CTGGTACACC GGAGTAGGGT GTGTCGGCCT 
ACCCGCCTGG CTCTGGAATC CCTCAGAACG TTACCATGTA TGAGCGAACA CCCCCTTCCA 
TCAGAGCCAC GCAGCCCAGG AGAGTACATC AACATAGACT TTAGCAGTGT TACCTGTGAC 
CCACAGGCAT CCACAGTGTC GGAGAGCTCT GAGTCTTCTG TGAGTTTAAC ATGTGGGAAG 
AGAGGATCCC TGCCACTCTC AGACTATGCT AATGTTGATG TCAGTTCTCC AGGTCACCTT 
GGCTCAACCC AAGCTAGGGT TTCCCCCACT GCAGGGTGCC TAAACCACAC ACCTGAAGTC 
TTGTCTTGTC CTCTGGCGAG CACCCAGCAG GGCATGCAGG AAGGCAAGGA GGAAGAAGGG 
GAAAAAAGCA AAGAGGGACA GACAGAGGAG AGGGGGATGG TAGAGGAGTA TCCTCACCAA 
CAGCAGGTGA GCCTGACGTG TCAGCCTGCT CCAGTTAAGG ATGACTACAC TGAGATGACT 
TTCAGTCCTC TTGCCCCTCT CCCTGCTCTC TGCACCACTG ATGCTGCTTC CCTCCCCACC 
AGCCCCACCG CATGTGTCCA GAGACTCAGC CTTGAAAGCA GCATTGTGGA AACGGTGCCC 
CATGTCCCAG AGCACTCTTT TCTCATGGGG GGTCCTTCGT TATCTGTCAC TCTCGTGGAT 
CCAAACAGGG GAGCCAAAGT GATCAAGGCT GATCCTCAAG GGCGTCGGCG GCACAGTTCT 
GAGACTTTTT CCTCCACCAC CACTGTCACA CCCGTGTGTC CATCCTTTGC CCACGACACC 
AAACGGCACA GCTCCGCCTC TGTGGAGAAT GTGTCCCGCA CTGTTCGCAG TTCTGAGGGA 
CCTGACGAGG ACTATGGCAA CAACAGCCCC ATGTGCAGGG AGACGTCAGC TGGTTATCAA 
AATGGACTTA ACTACATTGC CTTAAACTTG ATGGAGGGAA ACTTTGGAGG ATGCCGGTTG 
GGGGGCTGTG ATGGACTCTT GAGGTTCAAG CCCGCCTGTG GCTGCAAGGG TGGCATGAAT 
GGTTTCAACA CCAGCCCCTA TGCCAGCCTG GGATTCAAGG AGACTGCTGC TGCTGTGAAA 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022596278.1
CDS	183..3548
Translation	MASPPNTGDQSLIPSNTNANNAVKKCGYLKKQKHGHKRFFVLKEPSEGFPARLEYYESEKKWKNKSAAKRVIPLDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQESWYRVLTDLMAEGKVYDGSASNSASSLVGFDEASYGVITPVTAVYKEVWQVNLKSKGLGQSRNLTGVYRLCLSSRTISFVKLNSEVASVILQLMNIRRCGHSDSFFFIEVGRSAATGPGELWMQADDSVVAQNIHETILEAMKAMKELSEFRPRSKSQSSGTNPISVPTRRHLNNLPPSQTGLPRRSRTDSMAATSPVSKFSSCRIRTASEGDGTMTRPMSVSGSPLSPGVHRTLLSRSHTITARPCRTFESSSLQHSKSMSMPLSPSPPMATPSLVSLSSCPDSSAPRPSSCSASFSGSPSDAGFISCEEYGSSPADARDLRLPLTLRSNTPESLKADTPPSRDSSELEGYMVMERQNQNGYRRLPDLDKAYRKRTYSLTTPRQQRPPPQVSSASLDEYTLMRATYTSGSQSSRRCHTASPKGTYPEDYRDVHIGSNSVQADSGYMPMMPGVTPQTPGSKDDPYMPMSPMCVSAPKQIINPHSHSSSAGLALRTDSPGSVSLEESGYMRMWCGTKMSVESTDGKLTNGEYLNMSPVDPLVSLTPPDYILSPSGGDPHPQQHHRQPYFYSSLPRSLKGPLQRNGSTDTDQYVVMNLQRQRIEEESNYCPVSPRDSVASSSPVTSSAPSPLRLARLDGGLVHRSRVCRPTRLALESLRTLPCMSEHPLPSEPRSPGEYINIDFSSVTCDPQASTVSESSESSVSLTCGKRGSLPLSDYANVDVSSPGHLGSTQARVSPTAGCLNHTPEVLSCPLASTQQGMQEGKEEEGEKSKEGQTEERGMVEEYPHQQQVSLTCQPAPVKDDYTEMTFSPLAPLPALCTTDAASLPTSPTACVQRLSLESSIVETVPHVPEHSFLMGGPSLSVTLVDPNRGAKVIKADPQGRRRHSSETFSSTTTVTPVCPSFAHDTKRHSSASVENVSRTVRSSEGPDEDYGNNSPMCRETSAGYQNGLNYIALNLMEGNFGGCRLGGCDGLLRFKPACGCKGGMNGFNTSPYASLGFKETAAAVKE

Target ORF information:

RefSeq Version	XM_022740557.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili insulin receptor substrate 2 (irs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022740557.1

ORF Insert Sequence:

ATGGCGAGTC CGCCAAACAC GGGAGACCAG TCGTTAATAC CCAGCAATAC GAACGCGAAC 
AACGCTGTCA AGAAATGCGG ATACCTAAAG AAGCAGAAAC ACGGACACAA GCGCTTTTTT 
GTTTTGAAAG AGCCGAGCGA AGGGTTCCCT GCGCGGCTGG AGTACTACGA GAGCGAGAAG 
AAATGGAAAA ACAAATCGGC CGCGAAGAGA GTTATTCCCC TCGACTGCTG CCTCAATATC 
AACAAGAGAG CGGACGCAAA ACACAAATAT CTTATTGCTC TTTATACCAA GGACGAATAT 
TTTGCCGTAG CGGCAGAAAA CGAGCAGGAA CAAGAGAGCT GGTACCGGGT GCTGACGGAT 
TTAATGGCAG AGGGGAAAGT GTATGATGGC TCGGCGTCTA ACTCAGCGTC CTCGCTGGTT 
GGCTTCGACG AGGCGAGCTA CGGTGTAATA ACGCCGGTTA CCGCCGTCTA CAAGGAGGTA 
TGGCAAGTTA ATCTGAAATC TAAAGGCCTG GGGCAGAGCA GAAATTTGAC CGGGGTGTAC 
AGACTGTGTC TCTCCAGCCG GACTATCAGC TTCGTCAAGT TGAACTCCGA GGTAGCCTCC 
GTCATCTTAC AGCTGATGAA TATCCGGAGG TGCGGCCACT CTGACAGCTT CTTTTTCATT 
GAGGTGGGTC GTTCTGCAGC CACGGGTCCC GGGGAGTTAT GGATGCAGGC TGATGACTCT 
GTGGTGGCAC AAAACATCCA CGAAACTATC TTGGAGGCGA TGAAAGCCAT GAAGGAGCTG 
TCGGAATTCC GTCCGCGCAG CAAGAGCCAG TCGTCTGGGA CAAACCCAAT CTCTGTGCCC 
ACACGGAGGC ACCTGAACAA TCTCCCCCCC AGCCAGACCG GGCTTCCTCG GCGGTCGCGC 
ACTGACAGCA TGGCTGCGAC CTCTCCTGTG AGCAAATTTT CTTCCTGTCG AATACGCACG 
GCAAGCGAGG GCGATGGCAC CATGACACGC CCCATGTCAG TGAGTGGGAG CCCGCTTAGC 
CCGGGGGTCC ACAGGACCCT CCTGAGCAGA TCTCACACCA TCACAGCTCG CCCTTGTCGG 
ACATTTGAAT CCTCTTCCCT CCAGCACAGT AAGTCCATGT CTATGCCCCT GTCTCCCTCC 
CCACCCATGG CCACCCCTAG CCTAGTGAGT CTTTCCTCCT GCCCAGACAG CAGTGCCCCT 
CGCCCCTCCA GCTGCAGTGC CTCCTTCTCA GGCTCACCCA GTGATGCTGG TTTTATATCA 
TGTGAGGAGT ATGGCTCTAG TCCTGCAGAT GCACGGGACC TCAGGCTGCC ACTCACCCTC 
CGTAGCAACA CTCCCGAGTC CCTCAAAGCT GACACCCCCC CCTCCCGGGA CAGCAGTGAG 
CTTGAAGGCT ACATGGTGAT GGAGCGACAG AACCAGAATG GTTACCGTCG GTTACCGGAC 
CTGGACAAGG CATATCGAAA GCGCACATAC TCCCTCACCA CTCCCCGCCA GCAAAGGCCC 
CCTCCCCAAG TATCTTCAGC CTCTCTGGAT GAGTACACTC TTATGAGGGC CACGTACACC 
AGCGGAAGCC AGTCTTCTCG CAGGTGCCAC ACTGCCTCCC CTAAAGGGAC CTATCCTGAG 
GATTACAGGG ATGTCCATAT CGGATCTAAC AGTGTCCAAG CTGACAGTGG TTATATGCCC 
ATGATGCCAG GTGTGACACC TCAGACGCCA GGGTCTAAAG ATGACCCCTA CATGCCCATG 
AGCCCCATGT GTGTTTCTGC TCCAAAGCAG ATCATCAACC CCCATTCGCA CTCCTCTTCA 
GCAGGGCTGG CCCTACGCAC AGACTCCCCC GGGAGTGTTT CTCTGGAGGA GAGTGGCTAC 
ATGCGCATGT GGTGCGGAAC CAAGATGTCA GTGGAGAGCA CAGATGGAAA GCTCACCAAC 
GGAGAGTACC TGAATATGTC TCCTGTTGAC CCTCTAGTGT CTCTCACACC CCCAGACTAC 
ATCCTTAGTC CCTCAGGAGG AGATCCCCAC CCCCAACAAC ATCACAGACA GCCTTATTTT 
TATAGCTCTC TTCCACGCTC ACTTAAAGGC CCATTGCAGC GCAACGGGTC CACAGACACA 
GACCAGTACG TGGTGATGAA TTTACAGAGA CAGCGGATAG AAGAGGAGTC CAACTATTGT 
CCTGTCTCTC CACGAGACTC GGTGGCCAGC AGCTCTCCGG TGACCTCCTC TGCCCCATCT 
CCTCTCAGAC TGGCTCGGTT AGATGGAGGT CTGGTACACC GGAGTAGGGT GTGTCGGCCT 
ACCCGCCTGG CTCTGGAATC CCTCAGAACG TTACCATGTA TGAGCGAACA CCCCCTTCCA 
TCAGAGCCAC GCAGCCCAGG AGAGTACATC AACATAGACT TTAGCAGTGT TACCTGTGAC 
CCACAGGCAT CCACAGTGTC GGAGAGCTCT GAGTCTTCTG TGAGTTTAAC ATGTGGGAAG 
AGAGGATCCC TGCCACTCTC AGACTATGCT AATGTTGATG TCAGTTCTCC AGGTCACCTT 
GGCTCAACCC AAGCTAGGGT TTCCCCCACT GCAGGGTGCC TAAACCACAC ACCTGAAGTC 
TTGTCTTGTC CTCTGGCGAG CACCCAGCAG GGCATGCAGG AAGGCAAGGA GGAAGAAGGG 
GAAAAAAGCA AAGAGGGACA GACAGAGGAG AGGGGGATGG TAGAGGAGTA TCCTCACCAA 
CAGCAGGTGA GCCTGACGTG TCAGCCTGCT CCAGTTAAGG ATGACTACAC TGAGATGACT 
TTCAGTCCTC TTGCCCCTCT CCCTGCTCTC TGCACCACTG ATGCTGCTTC CCTCCCCACC 
AGCCCCACCG CATGTGTCCA GAGACTCAGC CTTGAAAGCA GCATTGTGGA AACGGTGCCC 
CATGTCCCAG AGCACTCTTT TCTCATGGGG GGTCCTTCGT TATCTGTCAC TCTCGTGGAT 
CCAAACAGGG GAGCCAAAGT GATCAAGGCT GATCCTCAAG GGCGTCGGCG GCACAGTTCT 
GAGACTTTTT CCTCCACCAC CACTGTCACA CCCGTGTGTC CATCCTTTGC CCACGACACC 
AAACGGCACA GCTCCGCCTC TGTGGAGAAT GTGTCCCGCA CTGTTCGCAG TTCTGAGGGA 
CCTGACGAGG ACTATGGCAA CAACAGCCCC ATGTGCAGGG AGACGTCAGC TGGTTATCAA 
AATGGACTTA ACTACATTGC CTTAAACTTG ATGGAGGGAA ACTTTGGAGG ATGCCGGTTG 
GGGGGCTGTG ATGGACTCTT GAGGTTCAAG CCCGCCTGTG GCTGCAAGGG TGGCATGAAT 
GGTTTCAACA CCAGCCCCTA TGCCAGCCTG GGATTCAAGG AGACTGCTGC TGCTGTGAAA 
GAATGA