med17 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	med17
Entrez Gene ID	111216966
Full Name	mediator complex subunit 17
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022738638.1	XP_022594359.1	mediator of RNA polymerase II transcription subunit 17

Pathways from BioSystems
Gene Ontology

The following med17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe210011	XM_022738638.1 Latest version!	Seriola dumerili mediator complex subunit 17 (med17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med17 ( XM_022738638.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022594359.1 Seriola dumerili mediator complex subunit 17 (med17), mRNA.

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CloneID	OSe210011
Clone ID Related Accession (Same CDS sequence)	XM_022738638.1
Accession Version	XM_022738638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	mediator of RNA polymerase II transcription subunit 17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GTCCAGCAGT GAGGGTCAGC ATCGAGTCTT CCTGTGAGAA GCAGGTGCAA 
GAAGTGGCCC TAGATGGGAC AGAGACATAT GTCCCCCCAC TGTCCATGTC ACAGAACTTG 
GCAAAGTTGG CACAAAGAAT TGACTTCAGC CAGGGATCTG ACTCAGAGGA GGACGGGGTG 
GAAGGTGAAC CCAGGGACCG TGAATGGGGC AAGCAGGAGC CAGAAGAAGA AGAAGGCACA 
GTGAAGTTTC AGCCATCACT TTGGCCCTGG GACTCGGTGC GTAACAACCT GCGCAGTGCC 
CTCACAGAGA TGTGTGTACT CTATGATGTG CTCAGCGTGG TGAAGGAGAA GAAGTACATG 
GCCTTAGACC CAGTGTCTCA GGATCCAACA GCTGGCAAGA CGCCCCAGGT GTTCCAGCTG 
ATCAGTAAAA AGAAGTCATT GGCCACAGCA GCGCAGCTCC TCCTGAAGGG AGCAGAGAAG 
CTCACTAAGT CAGTAGCAGA GAACCAGGAG AACAGGAGGC AGAGGGACTT CAACTCTGAA 
CTGCTGCGAC TCCGCTCACA GTGGAAACTA CGTAAAGTGG GAGACAAGAT CCTGGGAGAC 
CTCAGCTACC GCAGCGCAGG TTCCCTTTTT CCTCATCACG GCACGTTTGA GGTGATTAAG 
AACACAGACA TTGATCTGGA CAAAAAACTC CCAGAGGACT ACTGCCCCCT CGATGTCCAG 
ATCCCCAGTG ATTTAGAGGG ATCTGCTTAT ATCAAGGTGT CCATCCAGAA GCAAGCTCCA 
GACATCGGTG ATCTGGGAAC TGTCAACCTG TTCAGGAGAC CACCAAAAAC CAAAGCTGGT 
ACGCAGGCGT GGCATGTGAA GCTAGAGGCG GCTCAGAACG TTTTGCTGTG TAAGGAGATC 
TTCGCCCAGC TGTCCAGGGA AGCTGTCCAG ATTAAATCCC AGATCCCTCA CATTGTTGTG 
AAGAATCAGA TCATCTCGCA GCCCTTCCCA GGTCTGCAGC TGTCCATCTC ACTCTGCCAC 
TCTACTGGAG AGAAGAAGAA CCATAGGGCG TCTCCAGAGA AACCCAAACC AGACGACCAC 
CTTTACGTAC TGGAACATAA TCTGCATCAA ATGATGAGAG AGTTCCACAA GCAGCAGCTG 
AGCTCTGTGG TGATGCCACA TCCTGCCAGC GCCCCATTTG GCCACAAGCG CCTGCGCCTA 
GCGGGACCGC TGGCCTACGA CAAAACTGAA ATCTCCAGCT TGCAGCAGAG TGAAGGACTG 
CTGGAGAAGA TCATCAAACA GGCCAAACAC ATCTTCCTAC GCAGCAGGAC AGCACGGACC 
ATCGACAGCC TGGCCAGCCG GATCGAAGAC CCCCAGATCC AGGCTCACTG GTCGAATATC 
AACGATGTGT ATGAGTCCAG CGTCAAGGTG CTCATCACCT CTCAGGGCTA CGAGCAGATC 
TGCAAGTCCA TCCAGCTGCA GCTGAACATC GGTGTGGAGC AGATCAGAGT GGTGCACAGG 
GATGGACGTG TCATCACACT GTCACACCAG GAGCAGGAGC TGCAGGACTT CCTTCTCTCA 
CAGATGTCGC AGCATCAGGT GCATGCAGTT CAGCAGCTGG CCAAAGTGAT GGGCTGGCAC 
GTCCTGAGCT TCAGTAACCA TGTAGGCCTG GGCCCGGTCG AGAGCATTGG AAACGCCTCC 
GCTGTCACTG TCGCCTCTCC CAACGGAGAG TACGCCATCT CAGTGCGTAA CGGCCCAGAG 
AGCGGGTGTA AAGTTTTGGT CCAGTTCCCC CGCAGCCTGA CCAAAGAGCT GCCGAAGAGT 
GATGTCATCC AGGACACCAA GTGGAGTCAC CTCAGAGGGC CCAACAAAGA GGTCCACTGG 
AGCAAGATGG AGGGAAGAAA CTTTGTGTAC AAGATGGAGC TCCTCATGGC TGCACTGACT 
CCCTGCCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022594359.1
CDS	96..2027
Translation	MSGGPAVRVSIESSCEKQVQEVALDGTETYVPPLSMSQNLAKLAQRIDFSQGSDSEEDGVEGEPRDREWGKQEPEEEEGTVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSVVKEKKYMALDPVSQDPTAGKTPQVFQLISKKKSLATAAQLLLKGAEKLTKSVAENQENRRQRDFNSELLRLRSQWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDIDLDKKLPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFRRPPKTKAGTQAWHVKLEAAQNVLLCKEIFAQLSREAVQIKSQIPHIVVKNQIISQPFPGLQLSISLCHSTGEKKNHRASPEKPKPDDHLYVLEHNLHQMMREFHKQQLSSVVMPHPASAPFGHKRLRLAGPLAYDKTEISSLQQSEGLLEKIIKQAKHIFLRSRTARTIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWHVLSFSNHVGLGPVESIGNASAVTVASPNGEYAISVRNGPESGCKVLVQFPRSLTKELPKSDVIQDTKWSHLRGPNKEVHWSKMEGRNFVYKMELLMAALTPCP

Target ORF information:

RefSeq Version	XM_022738638.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili mediator complex subunit 17 (med17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022738638.1

ORF Insert Sequence:

ATGTCTGGTG GTCCAGCAGT GAGGGTCAGC ATCGAGTCTT CCTGTGAGAA GCAGGTGCAA 
GAAGTGGCCC TAGATGGGAC AGAGACATAT GTCCCCCCAC TGTCCATGTC ACAGAACTTG 
GCAAAGTTGG CACAAAGAAT TGACTTCAGC CAGGGATCTG ACTCAGAGGA GGACGGGGTG 
GAAGGTGAAC CCAGGGACCG TGAATGGGGC AAGCAGGAGC CAGAAGAAGA AGAAGGCACA 
GTGAAGTTTC AGCCATCACT TTGGCCCTGG GACTCGGTGC GTAACAACCT GCGCAGTGCC 
CTCACAGAGA TGTGTGTACT CTATGATGTG CTCAGCGTGG TGAAGGAGAA GAAGTACATG 
GCCTTAGACC CAGTGTCTCA GGATCCAACA GCTGGCAAGA CGCCCCAGGT GTTCCAGCTG 
ATCAGTAAAA AGAAGTCATT GGCCACAGCA GCGCAGCTCC TCCTGAAGGG AGCAGAGAAG 
CTCACTAAGT CAGTAGCAGA GAACCAGGAG AACAGGAGGC AGAGGGACTT CAACTCTGAA 
CTGCTGCGAC TCCGCTCACA GTGGAAACTA CGTAAAGTGG GAGACAAGAT CCTGGGAGAC 
CTCAGCTACC GCAGCGCAGG TTCCCTTTTT CCTCATCACG GCACGTTTGA GGTGATTAAG 
AACACAGACA TTGATCTGGA CAAAAAACTC CCAGAGGACT ACTGCCCCCT CGATGTCCAG 
ATCCCCAGTG ATTTAGAGGG ATCTGCTTAT ATCAAGGTGT CCATCCAGAA GCAAGCTCCA 
GACATCGGTG ATCTGGGAAC TGTCAACCTG TTCAGGAGAC CACCAAAAAC CAAAGCTGGT 
ACGCAGGCGT GGCATGTGAA GCTAGAGGCG GCTCAGAACG TTTTGCTGTG TAAGGAGATC 
TTCGCCCAGC TGTCCAGGGA AGCTGTCCAG ATTAAATCCC AGATCCCTCA CATTGTTGTG 
AAGAATCAGA TCATCTCGCA GCCCTTCCCA GGTCTGCAGC TGTCCATCTC ACTCTGCCAC 
TCTACTGGAG AGAAGAAGAA CCATAGGGCG TCTCCAGAGA AACCCAAACC AGACGACCAC 
CTTTACGTAC TGGAACATAA TCTGCATCAA ATGATGAGAG AGTTCCACAA GCAGCAGCTG 
AGCTCTGTGG TGATGCCACA TCCTGCCAGC GCCCCATTTG GCCACAAGCG CCTGCGCCTA 
GCGGGACCGC TGGCCTACGA CAAAACTGAA ATCTCCAGCT TGCAGCAGAG TGAAGGACTG 
CTGGAGAAGA TCATCAAACA GGCCAAACAC ATCTTCCTAC GCAGCAGGAC AGCACGGACC 
ATCGACAGCC TGGCCAGCCG GATCGAAGAC CCCCAGATCC AGGCTCACTG GTCGAATATC 
AACGATGTGT ATGAGTCCAG CGTCAAGGTG CTCATCACCT CTCAGGGCTA CGAGCAGATC 
TGCAAGTCCA TCCAGCTGCA GCTGAACATC GGTGTGGAGC AGATCAGAGT GGTGCACAGG 
GATGGACGTG TCATCACACT GTCACACCAG GAGCAGGAGC TGCAGGACTT CCTTCTCTCA 
CAGATGTCGC AGCATCAGGT GCATGCAGTT CAGCAGCTGG CCAAAGTGAT GGGCTGGCAC 
GTCCTGAGCT TCAGTAACCA TGTAGGCCTG GGCCCGGTCG AGAGCATTGG AAACGCCTCC 
GCTGTCACTG TCGCCTCTCC CAACGGAGAG TACGCCATCT CAGTGCGTAA CGGCCCAGAG 
AGCGGGTGTA AAGTTTTGGT CCAGTTCCCC CGCAGCCTGA CCAAAGAGCT GCCGAAGAGT 
GATGTCATCC AGGACACCAA GTGGAGTCAC CTCAGAGGGC CCAACAAAGA GGTCCACTGG 
AGCAAGATGG AGGGAAGAAA CTTTGTGTAC AAGATGGAGC TCCTCATGGC TGCACTGACT 
CCCTGCCCTT AG