sqle cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	sqle
Entrez Gene ID	108940515
Full Name	squalene epoxidase
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018762759.1	XP_018618275.1	squalene monooxygenase
XM_018762759.2	XP_018618275.1	squalene monooxygenase

Pathways from BioSystems
Gene Ontology

The following sqle gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sqle cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc62695	XM_018762759.2 Latest version!	Scleropages formosus squalene epoxidase (sqle), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OSc62695	XM_018762759.1	Scleropages formosus squalene epoxidase (sqle), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sqle ( XM_018762759.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018618275.1 Scleropages formosus squalene epoxidase (sqle), mRNA.

Price & Availability↑

CloneID	OSc62695
Clone ID Related Accession (Same CDS sequence)	XM_018762759.1 , XM_018762759.2
Accession Version	XM_018762759.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	squalene monooxygenase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372153.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACCT TTTTGGGCAT CGCGAGCTTC ACCTATATTT ACAAGAAGTG CGATGCCGTT 
CTCGGTCACG CGCATCGCGA GCTCGCGCTG GTGGTGCTTG CGCTGTTCAC CGCGTGCCTG 
CTGCTGTCAT ACGCGCGGTA CCGCGGCGGA GATGCGCTGC AGACGACGCT CGAGCTCCTG 
TCGTCGCTGC CGCTCGCGAG GCGACTTTTC CGCGTCAAAG AAGAACAAGA AGGAGGCTCC 
TTCGCTGAAA AGGTGTGTAG TCAAACAAAG GAGGTGGAAC AAATGGACTC AGATTGTAGA 
GAGAAGGTGG CAGCATGTGA CCCCTCGCAG GAAGCCGACA TCATCATCGT GGGGGCTGGT 
GTGCTAGGCT CTGCCATGGC TGCCGTGCTG GCACGAGACG GCCGCAAGGT TACCGTGGTG 
GAGAGAGATC TGCATGAGCC GGACAGAATT GTGGGCGAGC TGTTGCAACC GGGGGGATAC 
TGTGCCCTCA AAGATCTTGG GCTGGAAGGT TCAGTAGAGG GTTTGGATGC CCACGTTGTA 
AACGGCTACG TGATCCACGA CCTGGAGAGC CGCACGGAGG TGGAGATCCC ATACCCAAAG 
GAGGACAATG TAGTCCAGGG TGGGAGAGCT TTCCACCATG GGCGCTTCAT CATGGGCCTG 
CGGCGGGCTG CCATGGCTGA GACAAATGCA AAGTTGATTG AAGGCACAGT AACCAGCCTG 
CTGGAGCACA ATGGACGCAT CATAGGAATC CAGTACAGGG TGAAGGAAAC TGGGGAAGTG 
AAGGAGATTC ACGCCCCACT AACTGTGGTG GCCGATGGCT GTTTCTCCAA GTTCAGGAAA 
AACCTTGTGT CTAATAAAGT AAAAATTTCT TCACACTTTG TGGGATGCAT AATGAAGGAT 
TGTCCGCAGT TTAAGCCTCA CCATGCTGAA CTTGTGCTGG CTAATCCTAG CCCTGTGCTC 
ATTTACCAGA TCTCCTCCTC GGAAACCAGG GTTCTGGTTG ACATCCGAGG GGAGATGCCC 
AGAAACCTTA GGGAATACAT GGAGGAGAAG ATCTACCCAC AGCTGCCAGA GCACATCAAA 
GGGCCTTTCC TAAATGCTCT GCAGAACGAT CGCCTGAGGA CCATGCCAGC TAGCTTTCTC 
CCACCTTCTC CTGTAAACAA AGCAGGAGTC CTCCTTTTAG GGGATGCTTA CAACATGAGA 
CACCCCCTGA CTGGAGGAGG AATGAGTGTT GTCCTCAACG ATGTTAAGAT ATGGAGAGGG 
CTTCTGGGGA ACATCCCTGA CCTCTACGAT CAAGGCGGCA TGAAGCAGGC CAAGAAGAGG 
TTCCACTGGG AGCGGAAGTC ATCGCATTCG TTTGTGGTCA ACGTACTGGC ACAAGCTCTT 
TATGAACTGT TTGCTGCCAC AGACAATTCG CTACACCAGT TGAGGAAGGC CTGCTTCCAT 
TACTTCAAAC TTGGTGGAGA GTGTGTTTCT GGGCCTGTTG GACTCTTGTC AGTCCTGACG 
CCCAAACCCA TGACCCTGAT CGGGAATTTC TTCGCTGTGG CCTTGTACGC CATCTACTTC 
CGCTTCAAGT CTGAGTCCTG GCTGACGATG CCGCGAGCGT TATTGCACAG CGGCGAAATC 
CTGTACCGCG CCTGTACGGT CATGTTCCCC CTCATCTATT CTGAGCTCAA GTACCTGGTG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018618275.1
CDS	315..2000
Translation	MWTFLGIASFTYIYKKCDAVLGHAHRELALVVLALFTACLLLSYARYRGGDALQTTLELLSSLPLARRLFRVKEEQEGGSFAEKVCSQTKEVEQMDSDCREKVAACDPSQEADIIIVGAGVLGSAMAAVLARDGRKVTVVERDLHEPDRIVGELLQPGGYCALKDLGLEGSVEGLDAHVVNGYVIHDLESRTEVEIPYPKEDNVVQGGRAFHHGRFIMGLRRAAMAETNAKLIEGTVTSLLEHNGRIIGIQYRVKETGEVKEIHAPLTVVADGCFSKFRKNLVSNKVKISSHFVGCIMKDCPQFKPHHAELVLANPSPVLIYQISSSETRVLVDIRGEMPRNLREYMEEKIYPQLPEHIKGPFLNALQNDRLRTMPASFLPPSPVNKAGVLLLGDAYNMRHPLTGGGMSVVLNDVKIWRGLLGNIPDLYDQGGMKQAKKRFHWERKSSHSFVVNVLAQALYELFAATDNSLHQLRKACFHYFKLGGECVSGPVGLLSVLTPKPMTLIGNFFAVALYAIYFRFKSESWLTMPRALLHSGEILYRACTVMFPLIYSELKYLVY

Target ORF information:

RefSeq Version	XM_018762759.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus squalene epoxidase (sqle), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018762759.1

ORF Insert Sequence:

ATGTGGACCT TTTTGGGCAT CGCGAGCTTC ACCTATATTT ACAAGAAGTG CGATGCCGTT 
CTCGGTCACG CGCATCGCGA GCTCGCGCTG GTGGTGCTTG CGCTGTTCAC CGCGTGCCTG 
CTGCTGTCAT ACGCGCGGTA CCGCGGCGGA GATGCGCTGC AGACGACGCT CGAGCTCCTG 
TCGTCGCTGC CGCTCGCGAG GCGACTTTTC CGCGTCAAAG AAGAACAAGA AGGAGGCTCC 
TTCGCTGAAA AGGTGTGTAG TCAAACAAAG GAGGTGGAAC AAATGGACTC AGATTGTAGA 
GAGAAGGTGG CAGCATGTGA CCCCTCGCAG GAAGCCGACA TCATCATCGT GGGGGCTGGT 
GTGCTAGGCT CTGCCATGGC TGCCGTGCTG GCACGAGACG GCCGCAAGGT TACCGTGGTG 
GAGAGAGATC TGCATGAGCC GGACAGAATT GTGGGCGAGC TGTTGCAACC GGGGGGATAC 
TGTGCCCTCA AAGATCTTGG GCTGGAAGGT TCAGTAGAGG GTTTGGATGC CCACGTTGTA 
AACGGCTACG TGATCCACGA CCTGGAGAGC CGCACGGAGG TGGAGATCCC ATACCCAAAG 
GAGGACAATG TAGTCCAGGG TGGGAGAGCT TTCCACCATG GGCGCTTCAT CATGGGCCTG 
CGGCGGGCTG CCATGGCTGA GACAAATGCA AAGTTGATTG AAGGCACAGT AACCAGCCTG 
CTGGAGCACA ATGGACGCAT CATAGGAATC CAGTACAGGG TGAAGGAAAC TGGGGAAGTG 
AAGGAGATTC ACGCCCCACT AACTGTGGTG GCCGATGGCT GTTTCTCCAA GTTCAGGAAA 
AACCTTGTGT CTAATAAAGT AAAAATTTCT TCACACTTTG TGGGATGCAT AATGAAGGAT 
TGTCCGCAGT TTAAGCCTCA CCATGCTGAA CTTGTGCTGG CTAATCCTAG CCCTGTGCTC 
ATTTACCAGA TCTCCTCCTC GGAAACCAGG GTTCTGGTTG ACATCCGAGG GGAGATGCCC 
AGAAACCTTA GGGAATACAT GGAGGAGAAG ATCTACCCAC AGCTGCCAGA GCACATCAAA 
GGGCCTTTCC TAAATGCTCT GCAGAACGAT CGCCTGAGGA CCATGCCAGC TAGCTTTCTC 
CCACCTTCTC CTGTAAACAA AGCAGGAGTC CTCCTTTTAG GGGATGCTTA CAACATGAGA 
CACCCCCTGA CTGGAGGAGG AATGAGTGTT GTCCTCAACG ATGTTAAGAT ATGGAGAGGG 
CTTCTGGGGA ACATCCCTGA CCTCTACGAT CAAGGCGGCA TGAAGCAGGC CAAGAAGAGG 
TTCCACTGGG AGCGGAAGTC ATCGCATTCG TTTGTGGTCA ACGTACTGGC ACAAGCTCTT 
TATGAACTGT TTGCTGCCAC AGACAATTCG CTACACCAGT TGAGGAAGGC CTGCTTCCAT 
TACTTCAAAC TTGGTGGAGA GTGTGTTTCT GGGCCTGTTG GACTCTTGTC AGTCCTGACG 
CCCAAACCCA TGACCCTGAT CGGGAATTTC TTCGCTGTGG CCTTGTACGC CATCTACTTC 
CGCTTCAAGT CTGAGTCCTG GCTGACGATG CCGCGAGCGT TATTGCACAG CGGCGAAATC 
CTGTACCGCG CCTGTACGGT CATGTTCCCC CTCATCTATT CTGAGCTCAA GTACCTGGTG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqle ( XM_018762759.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018618275.1 Scleropages formosus squalene epoxidase (sqle), mRNA.

Price & Availability↑

CloneID	OSc62695
Clone ID Related Accession (Same CDS sequence)	XM_018762759.1 , XM_018762759.2
Accession Version	XM_018762759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	squalene monooxygenase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041823.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018762759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACCT TTTTGGGCAT CGCGAGCTTC ACCTATATTT ACAAGAAGTG CGATGCCGTT 
CTCGGTCACG CGCATCGCGA GCTCGCGCTG GTGGTGCTTG CGCTGTTCAC CGCGTGCCTG 
CTGCTGTCAT ACGCGCGGTA CCGCGGCGGA GATGCGCTGC AGACGACGCT CGAGCTCCTG 
TCGTCGCTGC CGCTCGCGAG GCGACTTTTC CGCGTCAAAG AAGAACAAGA AGGAGGCTCC 
TTCGCTGAAA AGGTGTGTAG TCAAACAAAG GAGGTGGAAC AAATGGACTC AGATTGTAGA 
GAGAAGGTGG CAGCATGTGA CCCCTCGCAG GAAGCCGACA TCATCATCGT GGGGGCTGGT 
GTGCTAGGCT CTGCCATGGC TGCCGTGCTG GCACGAGACG GCCGCAAGGT TACCGTGGTG 
GAGAGAGATC TGCATGAGCC GGACAGAATT GTGGGCGAGC TGTTGCAACC GGGGGGATAC 
TGTGCCCTCA AAGATCTTGG GCTGGAAGGT TCAGTAGAGG GTTTGGATGC CCACGTTGTA 
AACGGCTACG TGATCCACGA CCTGGAGAGC CGCACGGAGG TGGAGATCCC ATACCCAAAG 
GAGGACAATG TAGTCCAGGG TGGGAGAGCT TTCCACCATG GGCGCTTCAT CATGGGCCTG 
CGGCGGGCTG CCATGGCTGA GACAAATGCA AAGTTGATTG AAGGCACAGT AACCAGCCTG 
CTGGAGCACA ATGGACGCAT CATAGGAATC CAGTACAGGG TGAAGGAAAC TGGGGAAGTG 
AAGGAGATTC ACGCCCCACT AACTGTGGTG GCCGATGGCT GTTTCTCCAA GTTCAGGAAA 
AACCTTGTGT CTAATAAAGT AAAAATTTCT TCACACTTTG TGGGATGCAT AATGAAGGAT 
TGTCCGCAGT TTAAGCCTCA CCATGCTGAA CTTGTGCTGG CTAATCCTAG CCCTGTGCTC 
ATTTACCAGA TCTCCTCCTC GGAAACCAGG GTTCTGGTTG ACATCCGAGG GGAGATGCCC 
AGAAACCTTA GGGAATACAT GGAGGAGAAG ATCTACCCAC AGCTGCCAGA GCACATCAAA 
GGGCCTTTCC TAAATGCTCT GCAGAACGAT CGCCTGAGGA CCATGCCAGC TAGCTTTCTC 
CCACCTTCTC CTGTAAACAA AGCAGGAGTC CTCCTTTTAG GGGATGCTTA CAACATGAGA 
CACCCCCTGA CTGGAGGAGG AATGAGTGTT GTCCTCAACG ATGTTAAGAT ATGGAGAGGG 
CTTCTGGGGA ACATCCCTGA CCTCTACGAT CAAGGCGGCA TGAAGCAGGC CAAGAAGAGG 
TTCCACTGGG AGCGGAAGTC ATCGCATTCG TTTGTGGTCA ACGTACTGGC ACAAGCTCTT 
TATGAACTGT TTGCTGCCAC AGACAATTCG CTACACCAGT TGAGGAAGGC CTGCTTCCAT 
TACTTCAAAC TTGGTGGAGA GTGTGTTTCT GGGCCTGTTG GACTCTTGTC AGTCCTGACG 
CCCAAACCCA TGACCCTGAT CGGGAATTTC TTCGCTGTGG CCTTGTACGC CATCTACTTC 
CGCTTCAAGT CTGAGTCCTG GCTGACGATG CCGCGAGCGT TATTGCACAG CGGCGAAATC 
CTGTACCGCG CCTGTACGGT CATGTTCCCC CTCATCTATT CTGAGCTCAA GTACCTGGTG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018618275.1
CDS	315..2000
Translation	MWTFLGIASFTYIYKKCDAVLGHAHRELALVVLALFTACLLLSYARYRGGDALQTTLELLSSLPLARRLFRVKEEQEGGSFAEKVCSQTKEVEQMDSDCREKVAACDPSQEADIIIVGAGVLGSAMAAVLARDGRKVTVVERDLHEPDRIVGELLQPGGYCALKDLGLEGSVEGLDAHVVNGYVIHDLESRTEVEIPYPKEDNVVQGGRAFHHGRFIMGLRRAAMAETNAKLIEGTVTSLLEHNGRIIGIQYRVKETGEVKEIHAPLTVVADGCFSKFRKNLVSNKVKISSHFVGCIMKDCPQFKPHHAELVLANPSPVLIYQISSSETRVLVDIRGEMPRNLREYMEEKIYPQLPEHIKGPFLNALQNDRLRTMPASFLPPSPVNKAGVLLLGDAYNMRHPLTGGGMSVVLNDVKIWRGLLGNIPDLYDQGGMKQAKKRFHWERKSSHSFVVNVLAQALYELFAATDNSLHQLRKACFHYFKLGGECVSGPVGLLSVLTPKPMTLIGNFFAVALYAIYFRFKSESWLTMPRALLHSGEILYRACTVMFPLIYSELKYLVY

Target ORF information:

RefSeq Version	XM_018762759.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus squalene epoxidase (sqle), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018762759.2

ORF Insert Sequence:

ATGTGGACCT TTTTGGGCAT CGCGAGCTTC ACCTATATTT ACAAGAAGTG CGATGCCGTT 
CTCGGTCACG CGCATCGCGA GCTCGCGCTG GTGGTGCTTG CGCTGTTCAC CGCGTGCCTG 
CTGCTGTCAT ACGCGCGGTA CCGCGGCGGA GATGCGCTGC AGACGACGCT CGAGCTCCTG 
TCGTCGCTGC CGCTCGCGAG GCGACTTTTC CGCGTCAAAG AAGAACAAGA AGGAGGCTCC 
TTCGCTGAAA AGGTGTGTAG TCAAACAAAG GAGGTGGAAC AAATGGACTC AGATTGTAGA 
GAGAAGGTGG CAGCATGTGA CCCCTCGCAG GAAGCCGACA TCATCATCGT GGGGGCTGGT 
GTGCTAGGCT CTGCCATGGC TGCCGTGCTG GCACGAGACG GCCGCAAGGT TACCGTGGTG 
GAGAGAGATC TGCATGAGCC GGACAGAATT GTGGGCGAGC TGTTGCAACC GGGGGGATAC 
TGTGCCCTCA AAGATCTTGG GCTGGAAGGT TCAGTAGAGG GTTTGGATGC CCACGTTGTA 
AACGGCTACG TGATCCACGA CCTGGAGAGC CGCACGGAGG TGGAGATCCC ATACCCAAAG 
GAGGACAATG TAGTCCAGGG TGGGAGAGCT TTCCACCATG GGCGCTTCAT CATGGGCCTG 
CGGCGGGCTG CCATGGCTGA GACAAATGCA AAGTTGATTG AAGGCACAGT AACCAGCCTG 
CTGGAGCACA ATGGACGCAT CATAGGAATC CAGTACAGGG TGAAGGAAAC TGGGGAAGTG 
AAGGAGATTC ACGCCCCACT AACTGTGGTG GCCGATGGCT GTTTCTCCAA GTTCAGGAAA 
AACCTTGTGT CTAATAAAGT AAAAATTTCT TCACACTTTG TGGGATGCAT AATGAAGGAT 
TGTCCGCAGT TTAAGCCTCA CCATGCTGAA CTTGTGCTGG CTAATCCTAG CCCTGTGCTC 
ATTTACCAGA TCTCCTCCTC GGAAACCAGG GTTCTGGTTG ACATCCGAGG GGAGATGCCC 
AGAAACCTTA GGGAATACAT GGAGGAGAAG ATCTACCCAC AGCTGCCAGA GCACATCAAA 
GGGCCTTTCC TAAATGCTCT GCAGAACGAT CGCCTGAGGA CCATGCCAGC TAGCTTTCTC 
CCACCTTCTC CTGTAAACAA AGCAGGAGTC CTCCTTTTAG GGGATGCTTA CAACATGAGA 
CACCCCCTGA CTGGAGGAGG AATGAGTGTT GTCCTCAACG ATGTTAAGAT ATGGAGAGGG 
CTTCTGGGGA ACATCCCTGA CCTCTACGAT CAAGGCGGCA TGAAGCAGGC CAAGAAGAGG 
TTCCACTGGG AGCGGAAGTC ATCGCATTCG TTTGTGGTCA ACGTACTGGC ACAAGCTCTT 
TATGAACTGT TTGCTGCCAC AGACAATTCG CTACACCAGT TGAGGAAGGC CTGCTTCCAT 
TACTTCAAAC TTGGTGGAGA GTGTGTTTCT GGGCCTGTTG GACTCTTGTC AGTCCTGACG 
CCCAAACCCA TGACCCTGAT CGGGAATTTC TTCGCTGTGG CCTTGTACGC CATCTACTTC 
CGCTTCAAGT CTGAGTCCTG GCTGACGATG CCGCGAGCGT TATTGCACAG CGGCGAAATC 
CTGTACCGCG CCTGTACGGT CATGTTCCCC CTCATCTATT CTGAGCTCAA GTACCTGGTG 
TACTGA