Naa60 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Naa60
Entrez Gene ID	363545
Full Name	N(alpha)-acetyltransferase 60, NatF catalytic subunit
Synonyms	HAT4,Nat15,NatF,RGD1308915
General protein information	Preferred Names N(alpha)-acetyltransferase 60, NatF catalytic subunit Names N-alpha-acetyltransferase 60 GNAT acetyltransferase GNAT acetytransferase N-acetyltransferase 15 (GCN5-related, putative) N-alpha-acetyltransferase F histone acetyltransferase type B protein 4 natF catalytic subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767519.2	XP_008765741.1	N-alpha-acetyltransferase 60 isoform X1
XM_017597418.1	XP_017452907.1	N-alpha-acetyltransferase 60 isoform X1
XM_017597419.1	XP_017452908.1	N-alpha-acetyltransferase 60 isoform X2
NM_001014226.2	NP_001014248.2	N-alpha-acetyltransferase 60

Pathways from BioSystems

Homologies

Homo sapiens (human)	NAA60	NP_079121.1
Canis lupus familiaris (dog)	NAA60	XP_005621678.1
Bos taurus (cattle)	NAA60	NP_001069117.1
Danio rerio (zebrafish)	nat15	NP_001076341.1
Drosophila melanogaster (fruit fly)	Naa60	NP_996032.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005192	XP_314090.4
Mus musculus (house mouse)	Naa60	NP_083366.1
Xenopus tropicalis (tropical clawed frog)	naa60	NP_001106444.1
	NAT15	XP_001094891.1
Rattus norvegicus (Norway rat)	Naa60	NP_001014248.2
Gallus gallus (chicken)	NAA60	XP_414956.1
Caenorhabditis elegans (roundworm)	F30F8.10	NP_001122467.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase.
Chen JY, Liu L, Cao CL, Li MJ, Tan K, Yang X, Yun CH
Scientific reports631425(2016 Aug)

Crystal Structure of the Golgi-Associated Human N?-Acetyltransferase 60 Reveals the Molecular Determinants for Substrate-Specific Acetylation.
St?ve SI, Magin RS, Foyn H, Haug BE, Marmorstein R, Arnesen T
Structure (London, England : 1993)24(7)1044-56(2016 07)

An organellar n?-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, St?ve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T
Cell reports10(8)1362-74(2015 Mar)

HAT4, a Golgi apparatus-anchored B-type histone acetyltransferase, acetylates free histone H4 and facilitates chromatin assembly.
Yang X, Yu W, Shi L, Sun L, Liang J, Yi X, Li Q, Zhang Y, Yang F, Han X, Zhang D, Yang J, Yao Z, Shang Y
Molecular cell44(1)39-50(2011 Oct)

NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregation.
Van Damme P, Hole K, Pimenta-Marques A, Helsens K, Vandekerckhove J, Martinho RG, Gevaert K, Arnesen T
PLoS genetics7(7)e1002169(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Naa60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Naa60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12422	NM_001014226.2 Latest version!	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa48604	XM_008767519.2 Latest version!	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa48604	XM_017597418.1 Latest version!	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa57411	XM_017597419.1 Latest version!	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Naa60 ( NM_001014226.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014248.2 Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), mRNA.

Price & Availability↑

CloneID	ORa12422
Clone ID Related Accession (Same CDS sequence)	NM_001014226.2
Accession Version	NM_001014226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	N-alpha-acetyltransferase 60
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105304.1. On Sep 25, 2005 this sequence version replaced NM_001014226.1. ##Evidence-Data-START## Transcript exon combination :: BC105304.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END##

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
GATGGAGATA TTCTAGCCTC CAGCTTCTCT GTGGATACAC AAGTTGCATA CATCCTCAGT 
CTGGGAGTGG TGAAAGAGTT CAGGAAACAC GGCATAGGTT CCCTCTTATT AGAAAGTTTA 
AAGGATCATA TTTCAACCAC CGCCCAGGAC CACTGCAAAG CCATCTACCT GCACGTTCTC 
ACCACCAACA ACACAGCAAT AAACTTCTAT GAAAACAGAG ACTTTAGGCA GCATCACTAT 
CTGCCCTATT ACTACTCCAT CCGAGGGGTC CTCAAAGATG GCTTCACCTA TGTCCTCTAC 
ATCAATGGCG GCCACCCTCC CTGGACCATC TTAGACTACA TCCAGCACCT GGGCTCAGCA 
CTAGCCAACC TGAGCCCCTG CTCCATCCCA CACAGGATCT ACCGCCAGGC CCACAGCCTG 
CTTTGCAGCT TTCTGCCGTG GTCCAGCATT TCCAGCAAAG GTGGCATTGA ATACAGCCGT 
ACCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014248.2
CDS	110..838
Translation	MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYRDITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKEDGDILASSFSVDTQVAYILSLGVVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFRQHHYLPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALANLSPCSIPHRIYRQAHSLLCSFLPWSSISSKGGIEYSRTM

Target ORF information:

RefSeq Version	NM_001014226.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014226.2

ORF Insert Sequence:

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
GATGGAGATA TTCTAGCCTC CAGCTTCTCT GTGGATACAC AAGTTGCATA CATCCTCAGT 
CTGGGAGTGG TGAAAGAGTT CAGGAAACAC GGCATAGGTT CCCTCTTATT AGAAAGTTTA 
AAGGATCATA TTTCAACCAC CGCCCAGGAC CACTGCAAAG CCATCTACCT GCACGTTCTC 
ACCACCAACA ACACAGCAAT AAACTTCTAT GAAAACAGAG ACTTTAGGCA GCATCACTAT 
CTGCCCTATT ACTACTCCAT CCGAGGGGTC CTCAAAGATG GCTTCACCTA TGTCCTCTAC 
ATCAATGGCG GCCACCCTCC CTGGACCATC TTAGACTACA TCCAGCACCT GGGCTCAGCA 
CTAGCCAACC TGAGCCCCTG CTCCATCCCA CACAGGATCT ACCGCCAGGC CCACAGCCTG 
CTTTGCAGCT TTCTGCCGTG GTCCAGCATT TCCAGCAAAG GTGGCATTGA ATACAGCCGT 
ACCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naa60 ( XM_008767519.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765741.1 Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa48604
Clone ID Related Accession (Same CDS sequence)	XM_008767519.2 , XM_017597418.1
Accession Version	XM_008767519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-alpha-acetyltransferase 60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
ACAGGGATTC ACTGTGTCCT AGCTGGCCTG AAACTCAGAC CCGCCTGCCT CTGCCTCTCA 
AGTGCTGGAG TCCAAGATCG TGCGCCCATG CGCGGGCTGC TTGAGGTTGT CTCTTTAGAA 
GTGAAGGAAG TGAGTTTGAA GCAGCAGCTT GTGGGCAGAG ATGGAGATAT TCTAGCCTCC 
AGCTTCTCTG TGGATACACA AGTTGCATAC ATCCTCAGTC TGGGAGTGGT GAAAGAGTTC 
AGGAAACACG GCATAGGTTC CCTCTTATTA GAAAGTTTAA AGGATCATAT TTCAACCACC 
GCCCAGGACC ACTGCAAAGC CATCTACCTG CACGTTCTCA CCACCAACAA CACAGCAATA 
AACTTCTATG AAAACAGAGA CTTTAGGCAG CATCACTATC TGCCCTATTA CTACTCCATC 
CGAGGGGTCC TCAAAGATGG CTTCACCTAT GTCCTCTACA TCAATGGCGG CCACCCTCCC 
TGGACCATCT TAGACTACAT CCAGCACCTG GGCTCAGCAC TAGCCAACCT GAGCCCCTGC 
TCCATCCCAC ACAGGATCTA CCGCCAGGCC CACAGCCTGC TTTGCAGCTT TCTGCCGTGG 
TCCAGCATTT CCAGCAAAGG TGGCATTGAA TACAGCCGTA CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765741.1
CDS	484..1371
Translation	MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYRDITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKETGIHCVLAGLKLRPACLCLSSAGVQDRAPMRGLLEVVSLEVKEVSLKQQLVGRDGDILASSFSVDTQVAYILSLGVVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFRQHHYLPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALANLSPCSIPHRIYRQAHSLLCSFLPWSSISSKGGIEYSRTM

Target ORF information:

RefSeq Version	XM_008767519.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767519.2

ORF Insert Sequence:

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
ACAGGGATTC ACTGTGTCCT AGCTGGCCTG AAACTCAGAC CCGCCTGCCT CTGCCTCTCA 
AGTGCTGGAG TCCAAGATCG TGCGCCCATG CGCGGGCTGC TTGAGGTTGT CTCTTTAGAA 
GTGAAGGAAG TGAGTTTGAA GCAGCAGCTT GTGGGCAGAG ATGGAGATAT TCTAGCCTCC 
AGCTTCTCTG TGGATACACA AGTTGCATAC ATCCTCAGTC TGGGAGTGGT GAAAGAGTTC 
AGGAAACACG GCATAGGTTC CCTCTTATTA GAAAGTTTAA AGGATCATAT TTCAACCACC 
GCCCAGGACC ACTGCAAAGC CATCTACCTG CACGTTCTCA CCACCAACAA CACAGCAATA 
AACTTCTATG AAAACAGAGA CTTTAGGCAG CATCACTATC TGCCCTATTA CTACTCCATC 
CGAGGGGTCC TCAAAGATGG CTTCACCTAT GTCCTCTACA TCAATGGCGG CCACCCTCCC 
TGGACCATCT TAGACTACAT CCAGCACCTG GGCTCAGCAC TAGCCAACCT GAGCCCCTGC 
TCCATCCCAC ACAGGATCTA CCGCCAGGCC CACAGCCTGC TTTGCAGCTT TCTGCCGTGG 
TCCAGCATTT CCAGCAAAGG TGGCATTGAA TACAGCCGTA CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naa60 ( XM_017597418.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452907.1 Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa48604
Clone ID Related Accession (Same CDS sequence)	XM_008767519.2 , XM_017597418.1
Accession Version	XM_017597418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-alpha-acetyltransferase 60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
ACAGGGATTC ACTGTGTCCT AGCTGGCCTG AAACTCAGAC CCGCCTGCCT CTGCCTCTCA 
AGTGCTGGAG TCCAAGATCG TGCGCCCATG CGCGGGCTGC TTGAGGTTGT CTCTTTAGAA 
GTGAAGGAAG TGAGTTTGAA GCAGCAGCTT GTGGGCAGAG ATGGAGATAT TCTAGCCTCC 
AGCTTCTCTG TGGATACACA AGTTGCATAC ATCCTCAGTC TGGGAGTGGT GAAAGAGTTC 
AGGAAACACG GCATAGGTTC CCTCTTATTA GAAAGTTTAA AGGATCATAT TTCAACCACC 
GCCCAGGACC ACTGCAAAGC CATCTACCTG CACGTTCTCA CCACCAACAA CACAGCAATA 
AACTTCTATG AAAACAGAGA CTTTAGGCAG CATCACTATC TGCCCTATTA CTACTCCATC 
CGAGGGGTCC TCAAAGATGG CTTCACCTAT GTCCTCTACA TCAATGGCGG CCACCCTCCC 
TGGACCATCT TAGACTACAT CCAGCACCTG GGCTCAGCAC TAGCCAACCT GAGCCCCTGC 
TCCATCCCAC ACAGGATCTA CCGCCAGGCC CACAGCCTGC TTTGCAGCTT TCTGCCGTGG 
TCCAGCATTT CCAGCAAAGG TGGCATTGAA TACAGCCGTA CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452907.1
CDS	2699..3586
Translation	MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYRDITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKETGIHCVLAGLKLRPACLCLSSAGVQDRAPMRGLLEVVSLEVKEVSLKQQLVGRDGDILASSFSVDTQVAYILSLGVVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFRQHHYLPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALANLSPCSIPHRIYRQAHSLLCSFLPWSSISSKGGIEYSRTM

Target ORF information:

RefSeq Version	XM_017597418.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597418.1

ORF Insert Sequence:

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
ACAGGGATTC ACTGTGTCCT AGCTGGCCTG AAACTCAGAC CCGCCTGCCT CTGCCTCTCA 
AGTGCTGGAG TCCAAGATCG TGCGCCCATG CGCGGGCTGC TTGAGGTTGT CTCTTTAGAA 
GTGAAGGAAG TGAGTTTGAA GCAGCAGCTT GTGGGCAGAG ATGGAGATAT TCTAGCCTCC 
AGCTTCTCTG TGGATACACA AGTTGCATAC ATCCTCAGTC TGGGAGTGGT GAAAGAGTTC 
AGGAAACACG GCATAGGTTC CCTCTTATTA GAAAGTTTAA AGGATCATAT TTCAACCACC 
GCCCAGGACC ACTGCAAAGC CATCTACCTG CACGTTCTCA CCACCAACAA CACAGCAATA 
AACTTCTATG AAAACAGAGA CTTTAGGCAG CATCACTATC TGCCCTATTA CTACTCCATC 
CGAGGGGTCC TCAAAGATGG CTTCACCTAT GTCCTCTACA TCAATGGCGG CCACCCTCCC 
TGGACCATCT TAGACTACAT CCAGCACCTG GGCTCAGCAC TAGCCAACCT GAGCCCCTGC 
TCCATCCCAC ACAGGATCTA CCGCCAGGCC CACAGCCTGC TTTGCAGCTT TCTGCCGTGG 
TCCAGCATTT CCAGCAAAGG TGGCATTGAA TACAGCCGTA CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naa60 ( XM_017597419.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452908.1 Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa57411
Clone ID Related Accession (Same CDS sequence)	XM_017597419.1
Accession Version	XM_017597419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-alpha-acetyltransferase 60 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
ACAGGGATTC ACTGTGTCCT AGCTGGCCTG AAACTCAGAC CCGCCTGCCT CTGCCTCTCA 
AGTGCTGGAG TCCAAGATCG TGCGCCCATG CGCGGGCTGC TTGAGGTTGT CTCTTTAGAA 
GTGAAGGAAG ATGGAGATAT TCTAGCCTCC AGCTTCTCTG TGGATACACA AGTTGCATAC 
ATCCTCAGTC TGGGAGTGGT GAAAGAGTTC AGGAAACACG GCATAGGTTC CCTCTTATTA 
GAAAGTTTAA AGGATCATAT TTCAACCACC GCCCAGGACC ACTGCAAAGC CATCTACCTG 
CACGTTCTCA CCACCAACAA CACAGCAATA AACTTCTATG AAAACAGAGA CTTTAGGCAG 
CATCACTATC TGCCCTATTA CTACTCCATC CGAGGGGTCC TCAAAGATGG CTTCACCTAT 
GTCCTCTACA TCAATGGCGG CCACCCTCCC TGGACCATCT TAGACTACAT CCAGCACCTG 
GGCTCAGCAC TAGCCAACCT GAGCCCCTGC TCCATCCCAC ACAGGATCTA CCGCCAGGCC 
CACAGCCTGC TTTGCAGCTT TCTGCCGTGG TCCAGCATTT CCAGCAAAGG TGGCATTGAA 
TACAGCCGTA CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452908.1
CDS	484..1341
Translation	MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYRDITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKETGIHCVLAGLKLRPACLCLSSAGVQDRAPMRGLLEVVSLEVKEDGDILASSFSVDTQVAYILSLGVVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFRQHHYLPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALANLSPCSIPHRIYRQAHSLLCSFLPWSSISSKGGIEYSRTM

Target ORF information:

RefSeq Version	XM_017597419.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N(alpha)-acetyltransferase 60, NatF catalytic subunit (Naa60), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597419.1

ORF Insert Sequence:

ATGACAGAGG TGGTGCCTTC CAGCGCCCTC AGCGAGGTCA GCCTGCGCCT CCTCTGCCAC 
GATGACATAG ACACTGTGAA GCACCTGTGT GGCGACTGGT TCCCCATTGA GTACCCAGAC 
TCATGGTATC GTGATATCAC ATCCAACAAG AAGTTCTTCT CCCTTGCTGC AACCTACAGA 
GGTGCCATTG TGGGAATGAT AGTAGCTGAA ATAAAGAATA GGACCAAAAT ACATAAAGAG 
ACAGGGATTC ACTGTGTCCT AGCTGGCCTG AAACTCAGAC CCGCCTGCCT CTGCCTCTCA 
AGTGCTGGAG TCCAAGATCG TGCGCCCATG CGCGGGCTGC TTGAGGTTGT CTCTTTAGAA 
GTGAAGGAAG ATGGAGATAT TCTAGCCTCC AGCTTCTCTG TGGATACACA AGTTGCATAC 
ATCCTCAGTC TGGGAGTGGT GAAAGAGTTC AGGAAACACG GCATAGGTTC CCTCTTATTA 
GAAAGTTTAA AGGATCATAT TTCAACCACC GCCCAGGACC ACTGCAAAGC CATCTACCTG 
CACGTTCTCA CCACCAACAA CACAGCAATA AACTTCTATG AAAACAGAGA CTTTAGGCAG 
CATCACTATC TGCCCTATTA CTACTCCATC CGAGGGGTCC TCAAAGATGG CTTCACCTAT 
GTCCTCTACA TCAATGGCGG CCACCCTCCC TGGACCATCT TAGACTACAT CCAGCACCTG 
GGCTCAGCAC TAGCCAACCT GAGCCCCTGC TCCATCCCAC ACAGGATCTA CCGCCAGGCC 
CACAGCCTGC TTTGCAGCTT TCTGCCGTGG TCCAGCATTT CCAGCAAAGG TGGCATTGAA 
TACAGCCGTA CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase.
Scientific reports631425(2016 Aug)
Chen JY,Liu L,Cao CL,Li MJ,Tan K,Yang X,Yun CH

Crystal Structure of the Golgi-Associated Human N?-Acetyltransferase 60 Reveals the Molecular Determinants for Substrate-Specific Acetylation.
Structure (London, England : 1993)24(7)1044-56(2016 07)
St?ve SI,Magin RS,Foyn H,Haug BE,Marmorstein R,Arnesen T

An organellar n?-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
Cell reports10(8)1362-74(2015 Mar)
Aksnes H,Van Damme P,Goris M,Starheim KK,Marie M,St?ve SI,Hoel C,Kalvik TV,Hole K,Glomnes N,Furnes C,Ljostveit S,Ziegler M,Niere M,Gevaert K,Arnesen T

HAT4, a Golgi apparatus-anchored B-type histone acetyltransferase, acetylates free histone H4 and facilitates chromatin assembly.
Molecular cell44(1)39-50(2011 Oct)
Yang X,Yu W,Shi L,Sun L,Liang J,Yi X,Li Q,Zhang Y,Yang F,Han X,Zhang D,Yang J,Yao Z,Shang Y

NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregation.
PLoS genetics7(7)e1002169(2011 Jul)
Van Damme P,Hole K,Pimenta-Marques A,Helsens K,Vandekerckhove J,Martinho RG,Gevaert K,Arnesen T