Tdp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tdp1
Entrez Gene ID	314380
Full Name	tyrosyl-DNA phosphodiesterase 1
General protein information	Preferred Names tyrosyl-DNA phosphodiesterase 1 Names tyrosyl-DNA phosphodiesterase 1 tyr-DNA phosphodiesterase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031657.1	NP_001026827.1	tyrosyl-DNA phosphodiesterase 1
XM_006240432.3	XP_006240494.1	tyrosyl-DNA phosphodiesterase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TDP1	NP_001008744.1
Danio rerio (zebrafish)	tdp1	XP_700174.2
Xenopus tropicalis (tropical clawed frog)	tdp1	NP_001039242.1
	TDP1	XP_001089545.1
Canis lupus familiaris (dog)	TDP1	XP_547950.2
Bos taurus (cattle)	TDP1	NP_001180084.1
Gallus gallus (chicken)	TDP1	XP_004941916.1
Caenorhabditis elegans (roundworm)	F52C12.1	NP_500149.1
Oryza sativa (rice)	Os07g0530100	NP_001059844.1
Pan troglodytes (chimpanzee)	TDP1	NP_001233557.1
Mus musculus (house mouse)	Tdp1	NP_082630.2
Rattus norvegicus (Norway rat)	Tdp1	NP_001026827.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010577	XP_558110.3
Arabidopsis thaliana (thale cress)	TDP1	NP_197021.2
Drosophila melanogaster (fruit fly)	gkt	NP_523465.2

Gene Ontology
Bibliography

Related articles in PubMed

Biochemical characterization of human tyrosyl-DNA phosphodiesterase 2 (TDP2/TTRAP): a Mg(2+)/Mn(2+)-dependent phosphodiesterase specific for the repair of topoisomerase cleavage complexes.
Gao R, Huang SY, Marchand C, Pommier Y
The Journal of biological chemistry287(36)30842-52(2012 Aug)

DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair.
Gao Y, Katyal S, Lee Y, Zhao J, Rehg JE, Russell HR, McKinnon PJ
Nature471(7337)240-4(2011 Mar)

TDP1 facilitates chromosomal single-strand break repair in neurons and is neuroprotective in vivo.
Katyal S, el-Khamisy SF, Russell HR, Li Y, Ju L, Caldecott KW, McKinnon PJ
The EMBO journal26(22)4720-31(2007 Nov)

Spinocerebellar ataxia with axonal neuropathy: consequence of a Tdp1 recessive neomorphic mutation?
Hirano R, Interthal H, Huang C, Nakamura T, Deguchi K, Choi K, Bhattacharjee MB, Arimura K, Umehara F, Izumo S, Northrop JL, Salih MA, Inoue K, Armstrong DL, Champoux JJ, Takashima H, Boerkoel CF
The EMBO journal26(22)4732-43(2007 Nov)

TDP1 facilitates repair of ionizing radiation-induced DNA single-strand breaks.
El-Khamisy SF, Hartsuiker E, Caldecott KW
DNA repair6(10)1485-95(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tdp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tdp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08088	NM_001031657.1 Latest version!	Rattus norvegicus tyrosyl-DNA phosphodiesterase 1 (Tdp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$356.30 $509.00
ORa29532	XM_006240432.3 Latest version!	Rattus norvegicus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tdp1 ( NM_001031657.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001026827.1 Rattus norvegicus tyrosyl-DNA phosphodiesterase 1 (Tdp1), mRNA.

Price & Availability↑

CloneID	ORa08088
Clone ID Related Accession (Same CDS sequence)	NM_001031657.1
Accession Version	NM_001031657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	tyrosyl-DNA phosphodiesterase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098739.1. On Aug 12, 2005 this sequence version replaced XM_234379.3. ##Evidence-Data-START## Transcript exon combination :: BC098739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCAGG AAAGCAGCTA CGGAAAGTGG ACAATATCCA GTAGTGACGA CAGCGAGGAT 
GAAAAGCCCA AACCTGTCAA ACCATCAACA TCCTCTGGCC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC CCACGTACAC CTGCTCAGAG GCCCGCAAGG CTGCACACAA GAGACAGATC 
TCACCTGTGA AATTCAACAA CACAGACTCC GTGTTACCCC ACAAGAAGCA GAAGATGGAT 
TCCCCGGAAG GTCTGGGCTG GTGTCTGTCC AGCAGCGATG ATGAGCAGCA GCCGGATGTG 
ACACAGCAGG AGCAGCCTAA GGGAGTGCCA CCTCAAGAGA AAAAATATGC CCCCTCAGCC 
AATGTGACTA CTGCCCAGAA AGTTGAGGAT CGAAGCCCTC CTGACAGCCA TAGGGCCCAA 
AGGGCAGACG AAGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGAGAACC CCTTCCAGTT TTATCTCACT AGAGTCTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGATGTGAAC TGGCTCATAA AGCAATATCC ACCAGAATTC 
AGGAAGAAGC CGATCCTGCT TGTGCACGGC GATAAGAGGG AAGCCAAGGC AGACTTGCAT 
GCCCAGGCGA AGCCCTATGC AAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTCGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CACGAATTTA CCAGGGAAAC CACACATCCG GAGAGTCAAG CACCCATTTC 
AAAGCGGACC TCACCAGTTA TTTGATGGCG TATAATGCCC CCCCTCTCCA GGAATGGATA 
GACATCATCC AAGAACACGA TCTCTCTGAA ACCAATGTTT ACCTTATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAAAGATAAT TGGGGACATT TTAGACTTAG AAAGCTTCTG 
CAGGCGCATG CGCCATCTGC ACCCAGGGGC GAGTGCTGGC CCGTCGTGGG TCAGTTTTCA 
AGCATTGGCT CCCTGGGGCC TGATGAATCC AAGTGGTTGT GCTCAGAGTT TAAAGAGAGC 
TTGCTGGCAG TGAGAGAGGA AGGCCGGACC CCGGGCAGAA GCGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATATCCTGC TGGGGGCTCT 
CTTCCCTATG GCATCCAGAC GGCTGAGAAA CAGAGGTGGC TGCACCCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGCAAT GCCATGCCAC ATATTAAGAC GTACATGAGG 
CCCTCACCAG ACTTCAGTAA ACTGGCTTGG TTCCTTGTCA CAAGTGCAAA TCTGTCCAAG 
GCTGCCTGGG GAGCACTGGA GAAGAACGGC GCTCAACTGA TGATCCGCTC CTATGAGCTG 
GGGGTCCTTT TCCTCCCGTC GGCCTTTGGC CTGGACACCT TCAAAGTGAA ACAGAAATTC 
TTCTCCAGCA GCAGTGAGCC AATGGCCTCC TTTCCTGTGC CCTATGACCT GCCCCCAGAG 
CTCTACGGGA GTAAAGATCG GCCATGGATT TGGAACATTC CTTATGTCAA AGCACCAGAC 
ACACATGGCA ACATGTGGGT GCCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026827.1
CDS	17..1846
Translation	MSQESSYGKWTISSSDDSEDEKPKPVKPSTSSGPQAGQGVSKEPTYTCSEARKAAHKRQISPVKFNNTDSVLPHKKQKMDSPEGLGWCLSSSDDEQQPDVTQQEQPKGVPPQEKKYAPSANVTTAQKVEDRSPPDSHRAQRADEEYETSGEGQDIWDMLDKENPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVNWLIKQYPPEFRKKPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIYQGNHTSGESSTHFKADLTSYLMAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHKDNWGHFRLRKLLQAHAPSAPRGECWPVVGQFSSIGSLGPDESKWLCSEFKESLLAVREEGRTPGRSAVPLHLIYPSVENVRTSLEGYPAGGSLPYGIQTAEKQRWLHPYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGAQLMIRSYELGVLFLPSAFGLDTFKVKQKFFSSSSEPMASFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS

Target ORF information:

RefSeq Version	NM_001031657.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tyrosyl-DNA phosphodiesterase 1 (Tdp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031657.1

ORF Insert Sequence:

ATGTCCCAGG AAAGCAGCTA CGGAAAGTGG ACAATATCCA GTAGTGACGA CAGCGAGGAT 
GAAAAGCCCA AACCTGTCAA ACCATCAACA TCCTCTGGCC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC CCACGTACAC CTGCTCAGAG GCCCGCAAGG CTGCACACAA GAGACAGATC 
TCACCTGTGA AATTCAACAA CACAGACTCC GTGTTACCCC ACAAGAAGCA GAAGATGGAT 
TCCCCGGAAG GTCTGGGCTG GTGTCTGTCC AGCAGCGATG ATGAGCAGCA GCCGGATGTG 
ACACAGCAGG AGCAGCCTAA GGGAGTGCCA CCTCAAGAGA AAAAATATGC CCCCTCAGCC 
AATGTGACTA CTGCCCAGAA AGTTGAGGAT CGAAGCCCTC CTGACAGCCA TAGGGCCCAA 
AGGGCAGACG AAGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGAGAACC CCTTCCAGTT TTATCTCACT AGAGTCTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGATGTGAAC TGGCTCATAA AGCAATATCC ACCAGAATTC 
AGGAAGAAGC CGATCCTGCT TGTGCACGGC GATAAGAGGG AAGCCAAGGC AGACTTGCAT 
GCCCAGGCGA AGCCCTATGC AAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTCGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CACGAATTTA CCAGGGAAAC CACACATCCG GAGAGTCAAG CACCCATTTC 
AAAGCGGACC TCACCAGTTA TTTGATGGCG TATAATGCCC CCCCTCTCCA GGAATGGATA 
GACATCATCC AAGAACACGA TCTCTCTGAA ACCAATGTTT ACCTTATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAAAGATAAT TGGGGACATT TTAGACTTAG AAAGCTTCTG 
CAGGCGCATG CGCCATCTGC ACCCAGGGGC GAGTGCTGGC CCGTCGTGGG TCAGTTTTCA 
AGCATTGGCT CCCTGGGGCC TGATGAATCC AAGTGGTTGT GCTCAGAGTT TAAAGAGAGC 
TTGCTGGCAG TGAGAGAGGA AGGCCGGACC CCGGGCAGAA GCGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATATCCTGC TGGGGGCTCT 
CTTCCCTATG GCATCCAGAC GGCTGAGAAA CAGAGGTGGC TGCACCCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGCAAT GCCATGCCAC ATATTAAGAC GTACATGAGG 
CCCTCACCAG ACTTCAGTAA ACTGGCTTGG TTCCTTGTCA CAAGTGCAAA TCTGTCCAAG 
GCTGCCTGGG GAGCACTGGA GAAGAACGGC GCTCAACTGA TGATCCGCTC CTATGAGCTG 
GGGGTCCTTT TCCTCCCGTC GGCCTTTGGC CTGGACACCT TCAAAGTGAA ACAGAAATTC 
TTCTCCAGCA GCAGTGAGCC AATGGCCTCC TTTCCTGTGC CCTATGACCT GCCCCCAGAG 
CTCTACGGGA GTAAAGATCG GCCATGGATT TGGAACATTC CTTATGTCAA AGCACCAGAC 
ACACATGGCA ACATGTGGGT GCCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tdp1 ( XM_006240432.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240494.1 Rattus norvegicus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29532
Clone ID Related Accession (Same CDS sequence)	XM_006240432.3
Accession Version	XM_006240432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosyl-DNA phosphodiesterase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240432.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAAGA TCGGCATAGT CTGTGCAAGG ATGGCACACA GAAGTACAAT GTCCCAGGAA 
AGCAGCTACG GAAAGTGGAC AATATCCAGT AGTGACGACA GCGAGGATGA AAAGCCCAAA 
CCTGTCAAAC CATCAACATC CTCTGGCCCC CAGGCTGGGC AGGGAGTGTC GAAGGAGCCC 
ACGTACACCT GCTCAGAGGC CCGCAAGGCT GCACACAAGA GACAGATCTC ACCTGTGAAA 
TTCAACAACA CAGACTCCGT GTTACCCCAC AAGAAGCAGA AGATGGATTC CCCGGAAGGT 
CTGGGCTGGT GTCTGTCCAG CAGCGATGAT GAGCAGCAGC CGGATGTGAC ACAGCAGGAG 
CAGCCTAAGG GAGTGCCACC TCAAGAGAAA AAATATGCCC CCTCAGCCAA TGTGACTACT 
GCCCAGAAAG TTGAGGATCG AAGCCCTCCT GACAGCCATA GGGCCCAAAG GGCAGACGAA 
GAGTACGAGA CCTCAGGGGA AGGCCAGGAC ATCTGGGACA TGCTGGATAA GGAGAACCCC 
TTCCAGTTTT ATCTCACTAG AGTCTCGGGG ATTAAGGCAA AGTACAACTC CAAAGCCCTC 
CACATCAAGG ATATTTTATC CCCTCTGTTT GGGACACTTG TCTCTTCAGC TCAGTTTAAC 
TACTGCTTTG ATGTGAACTG GCTCATAAAG CAATATCCAC CAGAATTCAG GAAGAAGCCG 
ATCCTGCTTG TGCACGGCGA TAAGAGGGAA GCCAAGGCAG ACTTGCATGC CCAGGCGAAG 
CCCTATGCAA ACATTTCCCT CTGCCAGGCA AAGTTGGATA TTGCATTTGG AACACACCAC 
ACGAAAATGA TGCTCTTGCT CTATGAAGAA GGCCTCCGAG TCGTCATCCA CACCTCCAAC 
CTCATCCGGG AAGACTGGCA CCAGAAAACC CAAGGAATAT GGTTGAGCCC TTTGTACCCA 
CGAATTTACC AGGGAAACCA CACATCCGGA GAGTCAAGCA CCCATTTCAA AGCGGACCTC 
ACCAGTTATT TGATGGCGTA TAATGCCCCC CCTCTCCAGG AATGGATAGA CATCATCCAA 
GAACACGATC TCTCTGAAAC CAATGTTTAC CTTATTGGTT CAACCCCTGG ACGCTTTCAA 
GGAAGTCACA AAGATAATTG GGGACATTTT AGACTTAGAA AGCTTCTGCA GGCGCATGCG 
CCATCTGCAC CCAGGGGCGA GTGCTGGCCC GTCGTGGGTC AGTTTTCAAG CATTGGCTCC 
CTGGGGCCTG ATGAATCCAA GTGGTTGTGC TCAGAGTTTA AAGAGAGCTT GCTGGCAGTG 
AGAGAGGAAG GCCGGACCCC GGGCAGAAGC GCAGTTCCTC TTCATCTGAT CTATCCCTCT 
GTGGAAAATG TGCGGACCAG CTTAGAAGGA TATCCTGCTG GGGGCTCTCT TCCCTATGGC 
ATCCAGACGG CTGAGAAACA GAGGTGGCTG CACCCCTACT TCCACAAGTG GTCAGCAGAG 
ACATCCGGCC GAAGCAATGC CATGCCACAT ATTAAGACGT ACATGAGGCC CTCACCAGAC 
TTCAGTAAAC TGGCTTGGTT CCTTGTCACA AGTGCAAATC TGTCCAAGGC TGCCTGGGGA 
GCACTGGAGA AGAACGGCGC TCAACTGATG ATCCGCTCCT ATGAGCTGGG GGTCCTTTTC 
CTCCCGTCGG CCTTTGGCCT GGACACCTTC AAAGTGAAAC AGAAATTCTT CTCCAGCAGC 
AGTGAGCCAA TGGCCTCCTT TCCTGTGCCC TATGACCTGC CCCCAGAGCT CTACGGGAGT 
AAAGATCGGC CATGGATTTG GAACATTCCT TATGTCAAAG CACCAGACAC ACATGGCAAC 
ATGTGGGTGC CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240494.1
CDS	307..2184
Translation	MTKIGIVCARMAHRSTMSQESSYGKWTISSSDDSEDEKPKPVKPSTSSGPQAGQGVSKEPTYTCSEARKAAHKRQISPVKFNNTDSVLPHKKQKMDSPEGLGWCLSSSDDEQQPDVTQQEQPKGVPPQEKKYAPSANVTTAQKVEDRSPPDSHRAQRADEEYETSGEGQDIWDMLDKENPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVNWLIKQYPPEFRKKPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIYQGNHTSGESSTHFKADLTSYLMAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHKDNWGHFRLRKLLQAHAPSAPRGECWPVVGQFSSIGSLGPDESKWLCSEFKESLLAVREEGRTPGRSAVPLHLIYPSVENVRTSLEGYPAGGSLPYGIQTAEKQRWLHPYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGAQLMIRSYELGVLFLPSAFGLDTFKVKQKFFSSSSEPMASFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS

Target ORF information:

RefSeq Version	XM_006240432.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240432.3

ORF Insert Sequence:

ATGACGAAGA TCGGCATAGT CTGTGCAAGG ATGGCACACA GAAGTACAAT GTCCCAGGAA 
AGCAGCTACG GAAAGTGGAC AATATCCAGT AGTGACGACA GCGAGGATGA AAAGCCCAAA 
CCTGTCAAAC CATCAACATC CTCTGGCCCC CAGGCTGGGC AGGGAGTGTC GAAGGAGCCC 
ACGTACACCT GCTCAGAGGC CCGCAAGGCT GCACACAAGA GACAGATCTC ACCTGTGAAA 
TTCAACAACA CAGACTCCGT GTTACCCCAC AAGAAGCAGA AGATGGATTC CCCGGAAGGT 
CTGGGCTGGT GTCTGTCCAG CAGCGATGAT GAGCAGCAGC CGGATGTGAC ACAGCAGGAG 
CAGCCTAAGG GAGTGCCACC TCAAGAGAAA AAATATGCCC CCTCAGCCAA TGTGACTACT 
GCCCAGAAAG TTGAGGATCG AAGCCCTCCT GACAGCCATA GGGCCCAAAG GGCAGACGAA 
GAGTACGAGA CCTCAGGGGA AGGCCAGGAC ATCTGGGACA TGCTGGATAA GGAGAACCCC 
TTCCAGTTTT ATCTCACTAG AGTCTCGGGG ATTAAGGCAA AGTACAACTC CAAAGCCCTC 
CACATCAAGG ATATTTTATC CCCTCTGTTT GGGACACTTG TCTCTTCAGC TCAGTTTAAC 
TACTGCTTTG ATGTGAACTG GCTCATAAAG CAATATCCAC CAGAATTCAG GAAGAAGCCG 
ATCCTGCTTG TGCACGGCGA TAAGAGGGAA GCCAAGGCAG ACTTGCATGC CCAGGCGAAG 
CCCTATGCAA ACATTTCCCT CTGCCAGGCA AAGTTGGATA TTGCATTTGG AACACACCAC 
ACGAAAATGA TGCTCTTGCT CTATGAAGAA GGCCTCCGAG TCGTCATCCA CACCTCCAAC 
CTCATCCGGG AAGACTGGCA CCAGAAAACC CAAGGAATAT GGTTGAGCCC TTTGTACCCA 
CGAATTTACC AGGGAAACCA CACATCCGGA GAGTCAAGCA CCCATTTCAA AGCGGACCTC 
ACCAGTTATT TGATGGCGTA TAATGCCCCC CCTCTCCAGG AATGGATAGA CATCATCCAA 
GAACACGATC TCTCTGAAAC CAATGTTTAC CTTATTGGTT CAACCCCTGG ACGCTTTCAA 
GGAAGTCACA AAGATAATTG GGGACATTTT AGACTTAGAA AGCTTCTGCA GGCGCATGCG 
CCATCTGCAC CCAGGGGCGA GTGCTGGCCC GTCGTGGGTC AGTTTTCAAG CATTGGCTCC 
CTGGGGCCTG ATGAATCCAA GTGGTTGTGC TCAGAGTTTA AAGAGAGCTT GCTGGCAGTG 
AGAGAGGAAG GCCGGACCCC GGGCAGAAGC GCAGTTCCTC TTCATCTGAT CTATCCCTCT 
GTGGAAAATG TGCGGACCAG CTTAGAAGGA TATCCTGCTG GGGGCTCTCT TCCCTATGGC 
ATCCAGACGG CTGAGAAACA GAGGTGGCTG CACCCCTACT TCCACAAGTG GTCAGCAGAG 
ACATCCGGCC GAAGCAATGC CATGCCACAT ATTAAGACGT ACATGAGGCC CTCACCAGAC 
TTCAGTAAAC TGGCTTGGTT CCTTGTCACA AGTGCAAATC TGTCCAAGGC TGCCTGGGGA 
GCACTGGAGA AGAACGGCGC TCAACTGATG ATCCGCTCCT ATGAGCTGGG GGTCCTTTTC 
CTCCCGTCGG CCTTTGGCCT GGACACCTTC AAAGTGAAAC AGAAATTCTT CTCCAGCAGC 
AGTGAGCCAA TGGCCTCCTT TCCTGTGCCC TATGACCTGC CCCCAGAGCT CTACGGGAGT 
AAAGATCGGC CATGGATTTG GAACATTCCT TATGTCAAAG CACCAGACAC ACATGGCAAC 
ATGTGGGTGC CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biochemical characterization of human tyrosyl-DNA phosphodiesterase 2 (TDP2/TTRAP): a Mg(2+)/Mn(2+)-dependent phosphodiesterase specific for the repair of topoisomerase cleavage complexes.
The Journal of biological chemistry287(36)30842-52(2012 Aug)
Gao R,Huang SY,Marchand C,Pommier Y

DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair.
Nature471(7337)240-4(2011 Mar)
Gao Y,Katyal S,Lee Y,Zhao J,Rehg JE,Russell HR,McKinnon PJ

TDP1 facilitates chromosomal single-strand break repair in neurons and is neuroprotective in vivo.
The EMBO journal26(22)4720-31(2007 Nov)
Katyal S,el-Khamisy SF,Russell HR,Li Y,Ju L,Caldecott KW,McKinnon PJ

Spinocerebellar ataxia with axonal neuropathy: consequence of a Tdp1 recessive neomorphic mutation?
The EMBO journal26(22)4732-43(2007 Nov)
Hirano R,Interthal H,Huang C,Nakamura T,Deguchi K,Choi K,Bhattacharjee MB,Arimura K,Umehara F,Izumo S,Northrop JL,Salih MA,Inoue K,Armstrong DL,Champoux JJ,Takashima H,Boerkoel CF

TDP1 facilitates repair of ionizing radiation-induced DNA single-strand breaks.
DNA repair6(10)1485-95(2007 Oct)
El-Khamisy SF,Hartsuiker E,Caldecott KW