E2f8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	E2f8
Entrez Gene ID	308607
Full Name	E2F transcription factor 8
Synonyms	E2F-8,RGD1308091
General protein information	Preferred Names E2F transcription factor 8 Names transcription factor E2F8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229263.3	XP_006229325.1	transcription factor E2F8 isoform X1
XM_001080259.6	XP_001080259.1	transcription factor E2F8 isoform X2
NM_001100580.1	NP_001094050.1	transcription factor E2F8
XM_003753252.4	XP_003753300.1	transcription factor E2F8 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	E2F8	NP_001243300.1
	E2F8	XP_001095088.1
Bos taurus (cattle)	E2F8	NP_001178163.1
Pan troglodytes (chimpanzee)	E2F8	XP_508325.2
Gallus gallus (chicken)	E2F8	XP_420910.4
Mus musculus (house mouse)	E2f8	NP_001013386.2
Canis lupus familiaris (dog)	E2F8	XP_534087.2
Rattus norvegicus (Norway rat)	E2f8	XP_006229325.1
Danio rerio (zebrafish)	e2f8	XP_005168886.1
Xenopus tropicalis (tropical clawed frog)	e2f8	XP_002942881.1

Gene Ontology
Bibliography

Related articles in PubMed

E2F8 is essential for polyploidization in mammalian cells.
Pandit SK, Westendorp B, Nantasanti S, van Liere E, Tooten PC, Cornelissen PW, Toussaint MJ, Lamers WH, de Bruin A
Nature cell biology14(11)1181-91(2012 Nov)

Canonical and atypical E2Fs regulate the mammalian endocycle.
Chen HZ, Ouseph MM, Li J, P?cot T, Chokshi V, Kent L, Bae S, Byrne M, Duran C, Comstock G, Trikha P, Mair M, Senapati S, Martin CK, Gandhi S, Wilson N, Liu B, Huang YW, Thompson JC, Raman S, Singh S, Leone M, Machiraju R, Huang K, Mo X, Fernandez S, Kalaszczynska I, Wolgemuth DJ, Sicinski P, Huang T, Jin V, Leone G
Nature cell biology14(11)1192-202(2012 Nov)

E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1.
Weijts BG, Bakker WJ, Cornelissen PW, Liang KH, Schaftenaar FH, Westendorp B, de Wolf CA, Paciejewska M, Scheele CL, Kent L, Leone G, Schulte-Merker S, de Bruin A
The EMBO journal31(19)3871-84(2012 Oct)

Atypical E2F repressors and activators coordinate placental development.
Ouseph MM, Li J, Chen HZ, P?cot T, Wenzel P, Thompson JC, Comstock G, Chokshi V, Byrne M, Forde B, Chong JL, Huang K, Machiraju R, de Bruin A, Leone G
Developmental cell22(4)849-62(2012 Apr)

Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription.
Christensen J, Cloos P, Toftegaard U, Klinkenberg D, Bracken AP, Trinh E, Heeran M, Di Stefano L, Helin K
Nucleic acids research33(17)5458-70(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following E2f8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2f8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa14430	XM_003753252.4 Latest version!	Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa14430	XM_001080259.6 Latest version!	Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22836	XM_006229263.3 Latest version!	Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22836	NM_001100580.1 Latest version!	Rattus norvegicus E2F transcription factor 8 (E2f8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2f8 ( XM_001080259.6 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001080259.1 Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa14430
Clone ID Related Accession (Same CDS sequence)	XM_001080259.6 , XM_003753252.4
Accession Version	XM_001080259.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E2F8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001080259.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACAC 
TTATCTGGAG ATGAATTTGA GAAGTCCCAG CCAAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCTTAGC GAGGTACCCT AAGTACCCCA ACCCCGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTCGAAC GTCGGCGGAT TTATGACATC 
GTGAATGTAC TAGAGAGCCT GCACATGGTG AGCCGCCTCG CCAAAAACAG GTACACTTGG 
CACGGCCGGC ACAATCTCAC CAAAACCCTG GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCGG AGCAGATCAT GATGATCAAA AGGAAAGAGC ATGAGCAAGA GTTCGACTTC 
ATTAAGAGCT GTGGCCTAGA GGACCACGTG ATCAAGGGGG ACCACGTGAT CAAGTCAACC 
GCTGGCCAGA ACGGGCACTC AGACATGTGC TTCGTAGAGC TCCCTGGAGT GGAATTTCGG 
GCAGCTTCTG CAAACAGCCG CAAAGACAAG TCCTTACGCG TGATGAGCCA GAAGTTTGTG 
ATGCTGTTCT TGGTGTCGAC GCCTCAGATA GTAAGCCTGG AGATCGCTGC CAAGATCTTA 
ATTGGGGAGG ACCACGTGGA AGATCTGGAT AAAAGCAAGT TTAAAACAAA AATTAGGAGG 
CTGTACGATA TTGCTAATGT TCTGAGTAGC TTGGATCTTA TCAAGAAAGT TCATGTTACA 
GAAGAACGAG GACGGAAGCC AGCTTTCAAA TGGACAGGCC CGGAAATCAG CCCAAATAAC 
AGTGGCTCCA GCCCAGTCAT GCCTCTCACC GCCTCCTTAG AGGCTGAGCA GTCTGCAAAA 
GAGAACTGTG CCAAAAACCT CTTTTCTACA CGGGGGAAAC CCAGCTTTAC TCGACACCCA 
TCTCTTATCA AATTGGTAAA GAGCATAGAA AATGATCGGA GGAAGATCAG TTCCGCTCCC 
AGCAGCCCTG TCAAGAGCAG CAAAGCTGAG AGTTCTCAAA ATTCTCCACC CGTCCCAAAC 
AAAATGGCCC AGCTCGCTGC TATCTGCAAG ATGCAGTTGG AGGAGCAGTC AAGTGAACCC 
AGGAAGAGAG TAAAAGTCAA CCTAACAAGA TCTGGGCACT ACAAACCACT GGCTCCCCTG 
GACCCTGCAG TGAACACTGA GCTGGAACTG CTTGCTCCGT CCCTTATCCA GCCCCTGGGC 
ATGGTCCCCC TGATCCCTAG CCCATTGTCA TCTGCAGTGC CTGTGATCCT ACCTCAGGCC 
CCTTCGGGCC CATCCTATGC CATCTACTTG CAACCTGCCC AAGCCCAAAT GTTGACACCA 
CCCCATGGCC TGAGCCCAAC AGTCTGCCCC ACCCAGTCCT CTAATGCTAC CGGATCCAAA 
GACCCTACAG ACGCCCCCAC TGAGAAGACG GCCACGGATG CCACAAAGTC CAGTGCCTCC 
TGCAGACCCG GAAGCTTGCA GCCAGCACCA GAAAGGCAAG GTGCTAAGAA TCGAAGCAAG 
GAGACGACCG GAGATCGAGG CACGAAGAGG ACGGGCGCCT TGGAGGACGG TGGTCCCGGT 
CCCATAAAGA AACCTAAAGA GGACCTGAAG GCGCTTGAGA ATGTCCCCAC CCCCACGACC 
CTGTTCCCAT CAGGATACCT AATCCCTCTT ACCCAGTGCC CATCCCTGGG GCCAGACCCT 
ATGTTGTCTA ACACAGAAAA CTCAGGTACA CTTTCCCCAA ACCACAGGAT CTACGGCTCC 
CCAATTGCAG GTGTGATCCC AGTGGCGTCG TCTGAACTCA CTGCTGTGAA TTTTCCCCCA 
TTCCATGTGA CACCGCTGAA ACTTATGGTC TCCCCGACGT CTATGGCAGC CGTACCTGTT 
GGGAACAGCC CGGCTCTCAG CTCCAGCCAC CCTGCTCCCA CCCAGAACCC AAGCTCAGCC 
ATTGTAAACT TTACACTGCA GCACTTGGGA CTCATCTCCC CCGGCGTGCA GATGTCTGCC 
AGCCCCGGGC CTGGAGCTGG CACAGTCCCT CTGTCCCCAC GTGTAGAAGC AGCTAGCGTC 
ATACCAGATA ACTTGAGCTC TCGGCAAGGG AGGGCCACCA TCCATGACTC TCCAGTCCTG 
GGCCAAAGCC AACTAAATGG ACAACCAGTT GCTGGGACAG GGGCACAGCA GCCTGTTCCT 
GTGACGCCCA AAGGCTCCCA GTTGGTGGCT GAAAGTTTCT TCCGTACTCC AGGTGGACCA 
ACCAAGCCCA CCAGCTCATC TTTCATGGAT TTCGATGGTG CTAACAAAAC CTCCTTTGGA 
ACCCTCTTTG TCCCACAGCG CAAACTGGAA GTCTCAACTG AGGATGTCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001080259.1
CDS	824..3457
Translation	MENQKENLFSEPHKRGLVKSPLQESSKANVVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSGLPEARDCLHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEHEQEFDFIKSCGLEDHVIKGDHVIKSTAGQNGHSDMCFVELPGVEFRAASANSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPVMPLTASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSSKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKRVKVNLTRSGHYKPLAPLDPAVNTELELLAPSLIQPLGMVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPHGLSPTVCPTQSSNATGSKDPTDAPTEKTATDATKSSASCRPGSLQPAPERQGAKNRSKETTGDRGTKRTGALEDGGPGPIKKPKEDLKALENVPTPTTLFPSGYLIPLTQCPSLGPDPMLSNTENSGTLSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALSSSHPAPTQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPLSPRVEAASVIPDNLSSRQGRATIHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSFMDFDGANKTSFGTLFVPQRKLEVSTEDVH

Target ORF information:

RefSeq Version	XM_001080259.6
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001080259.6

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACAC 
TTATCTGGAG ATGAATTTGA GAAGTCCCAG CCAAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCTTAGC GAGGTACCCT AAGTACCCCA ACCCCGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTCGAAC GTCGGCGGAT TTATGACATC 
GTGAATGTAC TAGAGAGCCT GCACATGGTG AGCCGCCTCG CCAAAAACAG GTACACTTGG 
CACGGCCGGC ACAATCTCAC CAAAACCCTG GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCGG AGCAGATCAT GATGATCAAA AGGAAAGAGC ATGAGCAAGA GTTCGACTTC 
ATTAAGAGCT GTGGCCTAGA GGACCACGTG ATCAAGGGGG ACCACGTGAT CAAGTCAACC 
GCTGGCCAGA ACGGGCACTC AGACATGTGC TTCGTAGAGC TCCCTGGAGT GGAATTTCGG 
GCAGCTTCTG CAAACAGCCG CAAAGACAAG TCCTTACGCG TGATGAGCCA GAAGTTTGTG 
ATGCTGTTCT TGGTGTCGAC GCCTCAGATA GTAAGCCTGG AGATCGCTGC CAAGATCTTA 
ATTGGGGAGG ACCACGTGGA AGATCTGGAT AAAAGCAAGT TTAAAACAAA AATTAGGAGG 
CTGTACGATA TTGCTAATGT TCTGAGTAGC TTGGATCTTA TCAAGAAAGT TCATGTTACA 
GAAGAACGAG GACGGAAGCC AGCTTTCAAA TGGACAGGCC CGGAAATCAG CCCAAATAAC 
AGTGGCTCCA GCCCAGTCAT GCCTCTCACC GCCTCCTTAG AGGCTGAGCA GTCTGCAAAA 
GAGAACTGTG CCAAAAACCT CTTTTCTACA CGGGGGAAAC CCAGCTTTAC TCGACACCCA 
TCTCTTATCA AATTGGTAAA GAGCATAGAA AATGATCGGA GGAAGATCAG TTCCGCTCCC 
AGCAGCCCTG TCAAGAGCAG CAAAGCTGAG AGTTCTCAAA ATTCTCCACC CGTCCCAAAC 
AAAATGGCCC AGCTCGCTGC TATCTGCAAG ATGCAGTTGG AGGAGCAGTC AAGTGAACCC 
AGGAAGAGAG TAAAAGTCAA CCTAACAAGA TCTGGGCACT ACAAACCACT GGCTCCCCTG 
GACCCTGCAG TGAACACTGA GCTGGAACTG CTTGCTCCGT CCCTTATCCA GCCCCTGGGC 
ATGGTCCCCC TGATCCCTAG CCCATTGTCA TCTGCAGTGC CTGTGATCCT ACCTCAGGCC 
CCTTCGGGCC CATCCTATGC CATCTACTTG CAACCTGCCC AAGCCCAAAT GTTGACACCA 
CCCCATGGCC TGAGCCCAAC AGTCTGCCCC ACCCAGTCCT CTAATGCTAC CGGATCCAAA 
GACCCTACAG ACGCCCCCAC TGAGAAGACG GCCACGGATG CCACAAAGTC CAGTGCCTCC 
TGCAGACCCG GAAGCTTGCA GCCAGCACCA GAAAGGCAAG GTGCTAAGAA TCGAAGCAAG 
GAGACGACCG GAGATCGAGG CACGAAGAGG ACGGGCGCCT TGGAGGACGG TGGTCCCGGT 
CCCATAAAGA AACCTAAAGA GGACCTGAAG GCGCTTGAGA ATGTCCCCAC CCCCACGACC 
CTGTTCCCAT CAGGATACCT AATCCCTCTT ACCCAGTGCC CATCCCTGGG GCCAGACCCT 
ATGTTGTCTA ACACAGAAAA CTCAGGTACA CTTTCCCCAA ACCACAGGAT CTACGGCTCC 
CCAATTGCAG GTGTGATCCC AGTGGCGTCG TCTGAACTCA CTGCTGTGAA TTTTCCCCCA 
TTCCATGTGA CACCGCTGAA ACTTATGGTC TCCCCGACGT CTATGGCAGC CGTACCTGTT 
GGGAACAGCC CGGCTCTCAG CTCCAGCCAC CCTGCTCCCA CCCAGAACCC AAGCTCAGCC 
ATTGTAAACT TTACACTGCA GCACTTGGGA CTCATCTCCC CCGGCGTGCA GATGTCTGCC 
AGCCCCGGGC CTGGAGCTGG CACAGTCCCT CTGTCCCCAC GTGTAGAAGC AGCTAGCGTC 
ATACCAGATA ACTTGAGCTC TCGGCAAGGG AGGGCCACCA TCCATGACTC TCCAGTCCTG 
GGCCAAAGCC AACTAAATGG ACAACCAGTT GCTGGGACAG GGGCACAGCA GCCTGTTCCT 
GTGACGCCCA AAGGCTCCCA GTTGGTGGCT GAAAGTTTCT TCCGTACTCC AGGTGGACCA 
ACCAAGCCCA CCAGCTCATC TTTCATGGAT TTCGATGGTG CTAACAAAAC CTCCTTTGGA 
ACCCTCTTTG TCCCACAGCG CAAACTGGAA GTCTCAACTG AGGATGTCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2f8 ( XM_003753252.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003753300.1 Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa14430
Clone ID Related Accession (Same CDS sequence)	XM_001080259.6 , XM_003753252.4
Accession Version	XM_003753252.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E2F8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003753252.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACAC 
TTATCTGGAG ATGAATTTGA GAAGTCCCAG CCAAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCTTAGC GAGGTACCCT AAGTACCCCA ACCCCGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTCGAAC GTCGGCGGAT TTATGACATC 
GTGAATGTAC TAGAGAGCCT GCACATGGTG AGCCGCCTCG CCAAAAACAG GTACACTTGG 
CACGGCCGGC ACAATCTCAC CAAAACCCTG GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCGG AGCAGATCAT GATGATCAAA AGGAAAGAGC ATGAGCAAGA GTTCGACTTC 
ATTAAGAGCT GTGGCCTAGA GGACCACGTG ATCAAGGGGG ACCACGTGAT CAAGTCAACC 
GCTGGCCAGA ACGGGCACTC AGACATGTGC TTCGTAGAGC TCCCTGGAGT GGAATTTCGG 
GCAGCTTCTG CAAACAGCCG CAAAGACAAG TCCTTACGCG TGATGAGCCA GAAGTTTGTG 
ATGCTGTTCT TGGTGTCGAC GCCTCAGATA GTAAGCCTGG AGATCGCTGC CAAGATCTTA 
ATTGGGGAGG ACCACGTGGA AGATCTGGAT AAAAGCAAGT TTAAAACAAA AATTAGGAGG 
CTGTACGATA TTGCTAATGT TCTGAGTAGC TTGGATCTTA TCAAGAAAGT TCATGTTACA 
GAAGAACGAG GACGGAAGCC AGCTTTCAAA TGGACAGGCC CGGAAATCAG CCCAAATAAC 
AGTGGCTCCA GCCCAGTCAT GCCTCTCACC GCCTCCTTAG AGGCTGAGCA GTCTGCAAAA 
GAGAACTGTG CCAAAAACCT CTTTTCTACA CGGGGGAAAC CCAGCTTTAC TCGACACCCA 
TCTCTTATCA AATTGGTAAA GAGCATAGAA AATGATCGGA GGAAGATCAG TTCCGCTCCC 
AGCAGCCCTG TCAAGAGCAG CAAAGCTGAG AGTTCTCAAA ATTCTCCACC CGTCCCAAAC 
AAAATGGCCC AGCTCGCTGC TATCTGCAAG ATGCAGTTGG AGGAGCAGTC AAGTGAACCC 
AGGAAGAGAG TAAAAGTCAA CCTAACAAGA TCTGGGCACT ACAAACCACT GGCTCCCCTG 
GACCCTGCAG TGAACACTGA GCTGGAACTG CTTGCTCCGT CCCTTATCCA GCCCCTGGGC 
ATGGTCCCCC TGATCCCTAG CCCATTGTCA TCTGCAGTGC CTGTGATCCT ACCTCAGGCC 
CCTTCGGGCC CATCCTATGC CATCTACTTG CAACCTGCCC AAGCCCAAAT GTTGACACCA 
CCCCATGGCC TGAGCCCAAC AGTCTGCCCC ACCCAGTCCT CTAATGCTAC CGGATCCAAA 
GACCCTACAG ACGCCCCCAC TGAGAAGACG GCCACGGATG CCACAAAGTC CAGTGCCTCC 
TGCAGACCCG GAAGCTTGCA GCCAGCACCA GAAAGGCAAG GTGCTAAGAA TCGAAGCAAG 
GAGACGACCG GAGATCGAGG CACGAAGAGG ACGGGCGCCT TGGAGGACGG TGGTCCCGGT 
CCCATAAAGA AACCTAAAGA GGACCTGAAG GCGCTTGAGA ATGTCCCCAC CCCCACGACC 
CTGTTCCCAT CAGGATACCT AATCCCTCTT ACCCAGTGCC CATCCCTGGG GCCAGACCCT 
ATGTTGTCTA ACACAGAAAA CTCAGGTACA CTTTCCCCAA ACCACAGGAT CTACGGCTCC 
CCAATTGCAG GTGTGATCCC AGTGGCGTCG TCTGAACTCA CTGCTGTGAA TTTTCCCCCA 
TTCCATGTGA CACCGCTGAA ACTTATGGTC TCCCCGACGT CTATGGCAGC CGTACCTGTT 
GGGAACAGCC CGGCTCTCAG CTCCAGCCAC CCTGCTCCCA CCCAGAACCC AAGCTCAGCC 
ATTGTAAACT TTACACTGCA GCACTTGGGA CTCATCTCCC CCGGCGTGCA GATGTCTGCC 
AGCCCCGGGC CTGGAGCTGG CACAGTCCCT CTGTCCCCAC GTGTAGAAGC AGCTAGCGTC 
ATACCAGATA ACTTGAGCTC TCGGCAAGGG AGGGCCACCA TCCATGACTC TCCAGTCCTG 
GGCCAAAGCC AACTAAATGG ACAACCAGTT GCTGGGACAG GGGCACAGCA GCCTGTTCCT 
GTGACGCCCA AAGGCTCCCA GTTGGTGGCT GAAAGTTTCT TCCGTACTCC AGGTGGACCA 
ACCAAGCCCA CCAGCTCATC TTTCATGGAT TTCGATGGTG CTAACAAAAC CTCCTTTGGA 
ACCCTCTTTG TCCCACAGCG CAAACTGGAA GTCTCAACTG AGGATGTCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003753300.1
CDS	464..3097
Translation	MENQKENLFSEPHKRGLVKSPLQESSKANVVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSGLPEARDCLHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEHEQEFDFIKSCGLEDHVIKGDHVIKSTAGQNGHSDMCFVELPGVEFRAASANSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPVMPLTASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSSKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKRVKVNLTRSGHYKPLAPLDPAVNTELELLAPSLIQPLGMVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPHGLSPTVCPTQSSNATGSKDPTDAPTEKTATDATKSSASCRPGSLQPAPERQGAKNRSKETTGDRGTKRTGALEDGGPGPIKKPKEDLKALENVPTPTTLFPSGYLIPLTQCPSLGPDPMLSNTENSGTLSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALSSSHPAPTQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPLSPRVEAASVIPDNLSSRQGRATIHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSFMDFDGANKTSFGTLFVPQRKLEVSTEDVH

Target ORF information:

RefSeq Version	XM_003753252.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003753252.4

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACAC 
TTATCTGGAG ATGAATTTGA GAAGTCCCAG CCAAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCTTAGC GAGGTACCCT AAGTACCCCA ACCCCGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTCGAAC GTCGGCGGAT TTATGACATC 
GTGAATGTAC TAGAGAGCCT GCACATGGTG AGCCGCCTCG CCAAAAACAG GTACACTTGG 
CACGGCCGGC ACAATCTCAC CAAAACCCTG GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCGG AGCAGATCAT GATGATCAAA AGGAAAGAGC ATGAGCAAGA GTTCGACTTC 
ATTAAGAGCT GTGGCCTAGA GGACCACGTG ATCAAGGGGG ACCACGTGAT CAAGTCAACC 
GCTGGCCAGA ACGGGCACTC AGACATGTGC TTCGTAGAGC TCCCTGGAGT GGAATTTCGG 
GCAGCTTCTG CAAACAGCCG CAAAGACAAG TCCTTACGCG TGATGAGCCA GAAGTTTGTG 
ATGCTGTTCT TGGTGTCGAC GCCTCAGATA GTAAGCCTGG AGATCGCTGC CAAGATCTTA 
ATTGGGGAGG ACCACGTGGA AGATCTGGAT AAAAGCAAGT TTAAAACAAA AATTAGGAGG 
CTGTACGATA TTGCTAATGT TCTGAGTAGC TTGGATCTTA TCAAGAAAGT TCATGTTACA 
GAAGAACGAG GACGGAAGCC AGCTTTCAAA TGGACAGGCC CGGAAATCAG CCCAAATAAC 
AGTGGCTCCA GCCCAGTCAT GCCTCTCACC GCCTCCTTAG AGGCTGAGCA GTCTGCAAAA 
GAGAACTGTG CCAAAAACCT CTTTTCTACA CGGGGGAAAC CCAGCTTTAC TCGACACCCA 
TCTCTTATCA AATTGGTAAA GAGCATAGAA AATGATCGGA GGAAGATCAG TTCCGCTCCC 
AGCAGCCCTG TCAAGAGCAG CAAAGCTGAG AGTTCTCAAA ATTCTCCACC CGTCCCAAAC 
AAAATGGCCC AGCTCGCTGC TATCTGCAAG ATGCAGTTGG AGGAGCAGTC AAGTGAACCC 
AGGAAGAGAG TAAAAGTCAA CCTAACAAGA TCTGGGCACT ACAAACCACT GGCTCCCCTG 
GACCCTGCAG TGAACACTGA GCTGGAACTG CTTGCTCCGT CCCTTATCCA GCCCCTGGGC 
ATGGTCCCCC TGATCCCTAG CCCATTGTCA TCTGCAGTGC CTGTGATCCT ACCTCAGGCC 
CCTTCGGGCC CATCCTATGC CATCTACTTG CAACCTGCCC AAGCCCAAAT GTTGACACCA 
CCCCATGGCC TGAGCCCAAC AGTCTGCCCC ACCCAGTCCT CTAATGCTAC CGGATCCAAA 
GACCCTACAG ACGCCCCCAC TGAGAAGACG GCCACGGATG CCACAAAGTC CAGTGCCTCC 
TGCAGACCCG GAAGCTTGCA GCCAGCACCA GAAAGGCAAG GTGCTAAGAA TCGAAGCAAG 
GAGACGACCG GAGATCGAGG CACGAAGAGG ACGGGCGCCT TGGAGGACGG TGGTCCCGGT 
CCCATAAAGA AACCTAAAGA GGACCTGAAG GCGCTTGAGA ATGTCCCCAC CCCCACGACC 
CTGTTCCCAT CAGGATACCT AATCCCTCTT ACCCAGTGCC CATCCCTGGG GCCAGACCCT 
ATGTTGTCTA ACACAGAAAA CTCAGGTACA CTTTCCCCAA ACCACAGGAT CTACGGCTCC 
CCAATTGCAG GTGTGATCCC AGTGGCGTCG TCTGAACTCA CTGCTGTGAA TTTTCCCCCA 
TTCCATGTGA CACCGCTGAA ACTTATGGTC TCCCCGACGT CTATGGCAGC CGTACCTGTT 
GGGAACAGCC CGGCTCTCAG CTCCAGCCAC CCTGCTCCCA CCCAGAACCC AAGCTCAGCC 
ATTGTAAACT TTACACTGCA GCACTTGGGA CTCATCTCCC CCGGCGTGCA GATGTCTGCC 
AGCCCCGGGC CTGGAGCTGG CACAGTCCCT CTGTCCCCAC GTGTAGAAGC AGCTAGCGTC 
ATACCAGATA ACTTGAGCTC TCGGCAAGGG AGGGCCACCA TCCATGACTC TCCAGTCCTG 
GGCCAAAGCC AACTAAATGG ACAACCAGTT GCTGGGACAG GGGCACAGCA GCCTGTTCCT 
GTGACGCCCA AAGGCTCCCA GTTGGTGGCT GAAAGTTTCT TCCGTACTCC AGGTGGACCA 
ACCAAGCCCA CCAGCTCATC TTTCATGGAT TTCGATGGTG CTAACAAAAC CTCCTTTGGA 
ACCCTCTTTG TCCCACAGCG CAAACTGGAA GTCTCAACTG AGGATGTCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2f8 ( XM_006229263.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229325.1 Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.

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CloneID	ORa22836
Clone ID Related Accession (Same CDS sequence)	XM_006229263.3 , NM_001100580.1
Accession Version	XM_006229263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E2F8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACCA 
CAAGCGAAGA CGAACGAGAA GTCCCAGCCA AGTCGGAAGG AGAAGAGCTT GGGGTTGCTA 
TGCCACAAAT TCTTAGCGAG GTACCCTAAG TACCCCAACC CCGCTGTGAA TAACGACATC 
TGCCTGGACG AGGTGGCCGA GGAGCTCAAT GTCGAACGTC GGCGGATTTA TGACATCGTG 
AATGTACTAG AGAGCCTGCA CATGGTGAGC CGCCTCGCCA AAAACAGGTA CACTTGGCAC 
GGCCGGCACA ATCTCACCAA AACCCTGGGG ACGCTGAAGA GTGTCGGGGA AGAGAACAAG 
TACGCGGAGC AGATCATGAT GATCAAAAGG AAAGAGCATG AGCAAGAGTT CGACTTCATT 
AAGAGCTGTG GCCTAGAGGA CCACGTGATC AAGGGGGACC ACGTGATCAA GTCAACCGCT 
GGCCAGAACG GGCACTCAGA CATGTGCTTC GTAGAGCTCC CTGGAGTGGA ATTTCGGGCA 
GCTTCTGCAA ACAGCCGCAA AGACAAGTCC TTACGCGTGA TGAGCCAGAA GTTTGTGATG 
CTGTTCTTGG TGTCGACGCC TCAGATAGTA AGCCTGGAGA TCGCTGCCAA GATCTTAATT 
GGGGAGGACC ACGTGGAAGA TCTGGATAAA AGCAAGTTTA AAACAAAAAT TAGGAGGCTG 
TACGATATTG CTAATGTTCT GAGTAGCTTG GATCTTATCA AGAAAGTTCA TGTTACAGAA 
GAACGAGGAC GGAAGCCAGC TTTCAAATGG ACAGGCCCGG AAATCAGCCC AAATAACAGT 
GGCTCCAGCC CAGTCATGCC TCTCACCGCC TCCTTAGAGG CTGAGCAGTC TGCAAAAGAG 
AACTGTGCCA AAAACCTCTT TTCTACACGG GGGAAACCCA GCTTTACTCG ACACCCATCT 
CTTATCAAAT TGGTAAAGAG CATAGAAAAT GATCGGAGGA AGATCAGTTC CGCTCCCAGC 
AGCCCTGTCA AGAGCAGCAA AGCTGAGAGT TCTCAAAATT CTCCACCCGT CCCAAACAAA 
ATGGCCCAGC TCGCTGCTAT CTGCAAGATG CAGTTGGAGG AGCAGTCAAG TGAACCCAGG 
AAGAGAGTAA AAGTCAACCT AACAAGATCT GGGCACTACA AACCACTGGC TCCCCTGGAC 
CCTGCAGTGA ACACTGAGCT GGAACTGCTT GCTCCGTCCC TTATCCAGCC CCTGGGCATG 
GTCCCCCTGA TCCCTAGCCC ATTGTCATCT GCAGTGCCTG TGATCCTACC TCAGGCCCCT 
TCGGGCCCAT CCTATGCCAT CTACTTGCAA CCTGCCCAAG CCCAAATGTT GACACCACCC 
CATGGCCTGA GCCCAACAGT CTGCCCCACC CAGTCCTCTA ATGCTACCGG ATCCAAAGAC 
CCTACAGACG CCCCCACTGA GAAGACGGCC ACGGATGCCA CAAAGTCCAG TGCCTCCTGC 
AGACCCGGAA GCTTGCAGCC AGCACCAGAA AGGCAAGGTG CTAAGAATCG AAGCAAGGAG 
ACGACCGGAG ATCGAGGCAC GAAGAGGACG GGCGCCTTGG AGGACGGTGG TCCCGGTCCC 
ATAAAGAAAC CTAAAGAGGA CCTGAAGGCG CTTGAGAATG TCCCCACCCC CACGACCCTG 
TTCCCATCAG GATACCTAAT CCCTCTTACC CAGTGCCCAT CCCTGGGGCC AGACCCTATG 
TTGTCTAACA CAGAAAACTC AGGTACACTT TCCCCAAACC ACAGGATCTA CGGCTCCCCA 
ATTGCAGGTG TGATCCCAGT GGCGTCGTCT GAACTCACTG CTGTGAATTT TCCCCCATTC 
CATGTGACAC CGCTGAAACT TATGGTCTCC CCGACGTCTA TGGCAGCCGT ACCTGTTGGG 
AACAGCCCGG CTCTCAGCTC CAGCCACCCT GCTCCCACCC AGAACCCAAG CTCAGCCATT 
GTAAACTTTA CACTGCAGCA CTTGGGACTC ATCTCCCCCG GCGTGCAGAT GTCTGCCAGC 
CCCGGGCCTG GAGCTGGCAC AGTCCCTCTG TCCCCACGTG TAGAAGCAGC TAGCGTCATA 
CCAGATAACT TGAGCTCTCG GCAAGGGAGG GCCACCATCC ATGACTCTCC AGTCCTGGGC 
CAAAGCCAAC TAAATGGACA ACCAGTTGCT GGGACAGGGG CACAGCAGCC TGTTCCTGTG 
ACGCCCAAAG GCTCCCAGTT GGTGGCTGAA AGTTTCTTCC GTACTCCAGG TGGACCAACC 
AAGCCCACCA GCTCATCTTT CATGGATTTC GATGGTGCTA ACAAAACCTC CTTTGGAACC 
CTCTTTGTCC CACAGCGCAA ACTGGAAGTC TCAACTGAGG ATGTCCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229325.1
CDS	826..3456
Translation	MENQKENLFSEPHKRGLVKSPLQESSKANVVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSGLPEARDCLHEPQAKTNEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEHEQEFDFIKSCGLEDHVIKGDHVIKSTAGQNGHSDMCFVELPGVEFRAASANSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPVMPLTASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSSKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKRVKVNLTRSGHYKPLAPLDPAVNTELELLAPSLIQPLGMVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPHGLSPTVCPTQSSNATGSKDPTDAPTEKTATDATKSSASCRPGSLQPAPERQGAKNRSKETTGDRGTKRTGALEDGGPGPIKKPKEDLKALENVPTPTTLFPSGYLIPLTQCPSLGPDPMLSNTENSGTLSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALSSSHPAPTQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPLSPRVEAASVIPDNLSSRQGRATIHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSFMDFDGANKTSFGTLFVPQRKLEVSTEDVH

Target ORF information:

RefSeq Version	XM_006229263.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229263.3

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACCA 
CAAGCGAAGA CGAACGAGAA GTCCCAGCCA AGTCGGAAGG AGAAGAGCTT GGGGTTGCTA 
TGCCACAAAT TCTTAGCGAG GTACCCTAAG TACCCCAACC CCGCTGTGAA TAACGACATC 
TGCCTGGACG AGGTGGCCGA GGAGCTCAAT GTCGAACGTC GGCGGATTTA TGACATCGTG 
AATGTACTAG AGAGCCTGCA CATGGTGAGC CGCCTCGCCA AAAACAGGTA CACTTGGCAC 
GGCCGGCACA ATCTCACCAA AACCCTGGGG ACGCTGAAGA GTGTCGGGGA AGAGAACAAG 
TACGCGGAGC AGATCATGAT GATCAAAAGG AAAGAGCATG AGCAAGAGTT CGACTTCATT 
AAGAGCTGTG GCCTAGAGGA CCACGTGATC AAGGGGGACC ACGTGATCAA GTCAACCGCT 
GGCCAGAACG GGCACTCAGA CATGTGCTTC GTAGAGCTCC CTGGAGTGGA ATTTCGGGCA 
GCTTCTGCAA ACAGCCGCAA AGACAAGTCC TTACGCGTGA TGAGCCAGAA GTTTGTGATG 
CTGTTCTTGG TGTCGACGCC TCAGATAGTA AGCCTGGAGA TCGCTGCCAA GATCTTAATT 
GGGGAGGACC ACGTGGAAGA TCTGGATAAA AGCAAGTTTA AAACAAAAAT TAGGAGGCTG 
TACGATATTG CTAATGTTCT GAGTAGCTTG GATCTTATCA AGAAAGTTCA TGTTACAGAA 
GAACGAGGAC GGAAGCCAGC TTTCAAATGG ACAGGCCCGG AAATCAGCCC AAATAACAGT 
GGCTCCAGCC CAGTCATGCC TCTCACCGCC TCCTTAGAGG CTGAGCAGTC TGCAAAAGAG 
AACTGTGCCA AAAACCTCTT TTCTACACGG GGGAAACCCA GCTTTACTCG ACACCCATCT 
CTTATCAAAT TGGTAAAGAG CATAGAAAAT GATCGGAGGA AGATCAGTTC CGCTCCCAGC 
AGCCCTGTCA AGAGCAGCAA AGCTGAGAGT TCTCAAAATT CTCCACCCGT CCCAAACAAA 
ATGGCCCAGC TCGCTGCTAT CTGCAAGATG CAGTTGGAGG AGCAGTCAAG TGAACCCAGG 
AAGAGAGTAA AAGTCAACCT AACAAGATCT GGGCACTACA AACCACTGGC TCCCCTGGAC 
CCTGCAGTGA ACACTGAGCT GGAACTGCTT GCTCCGTCCC TTATCCAGCC CCTGGGCATG 
GTCCCCCTGA TCCCTAGCCC ATTGTCATCT GCAGTGCCTG TGATCCTACC TCAGGCCCCT 
TCGGGCCCAT CCTATGCCAT CTACTTGCAA CCTGCCCAAG CCCAAATGTT GACACCACCC 
CATGGCCTGA GCCCAACAGT CTGCCCCACC CAGTCCTCTA ATGCTACCGG ATCCAAAGAC 
CCTACAGACG CCCCCACTGA GAAGACGGCC ACGGATGCCA CAAAGTCCAG TGCCTCCTGC 
AGACCCGGAA GCTTGCAGCC AGCACCAGAA AGGCAAGGTG CTAAGAATCG AAGCAAGGAG 
ACGACCGGAG ATCGAGGCAC GAAGAGGACG GGCGCCTTGG AGGACGGTGG TCCCGGTCCC 
ATAAAGAAAC CTAAAGAGGA CCTGAAGGCG CTTGAGAATG TCCCCACCCC CACGACCCTG 
TTCCCATCAG GATACCTAAT CCCTCTTACC CAGTGCCCAT CCCTGGGGCC AGACCCTATG 
TTGTCTAACA CAGAAAACTC AGGTACACTT TCCCCAAACC ACAGGATCTA CGGCTCCCCA 
ATTGCAGGTG TGATCCCAGT GGCGTCGTCT GAACTCACTG CTGTGAATTT TCCCCCATTC 
CATGTGACAC CGCTGAAACT TATGGTCTCC CCGACGTCTA TGGCAGCCGT ACCTGTTGGG 
AACAGCCCGG CTCTCAGCTC CAGCCACCCT GCTCCCACCC AGAACCCAAG CTCAGCCATT 
GTAAACTTTA CACTGCAGCA CTTGGGACTC ATCTCCCCCG GCGTGCAGAT GTCTGCCAGC 
CCCGGGCCTG GAGCTGGCAC AGTCCCTCTG TCCCCACGTG TAGAAGCAGC TAGCGTCATA 
CCAGATAACT TGAGCTCTCG GCAAGGGAGG GCCACCATCC ATGACTCTCC AGTCCTGGGC 
CAAAGCCAAC TAAATGGACA ACCAGTTGCT GGGACAGGGG CACAGCAGCC TGTTCCTGTG 
ACGCCCAAAG GCTCCCAGTT GGTGGCTGAA AGTTTCTTCC GTACTCCAGG TGGACCAACC 
AAGCCCACCA GCTCATCTTT CATGGATTTC GATGGTGCTA ACAAAACCTC CTTTGGAACC 
CTCTTTGTCC CACAGCGCAA ACTGGAAGTC TCAACTGAGG ATGTCCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2f8 ( NM_001100580.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094050.1 Rattus norvegicus E2F transcription factor 8 (E2f8), mRNA.

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CloneID	ORa22836
Clone ID Related Accession (Same CDS sequence)	XM_006229263.3 , NM_001100580.1
Accession Version	NM_001100580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E2F8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07003303.1. On or before Nov 30, 2019 this sequence version replaced XM_006229263.3, XM_001080259.6. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2689594, SAMEA2689598 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACCA 
CAAGCGAAGA CGAACGAGAA GTCCCAGCCA AGTCGGAAGG AGAAGAGCTT GGGGTTGCTA 
TGCCACAAAT TCTTAGCGAG GTACCCTAAG TACCCCAACC CCGCTGTGAA TAACGACATC 
TGCCTGGACG AGGTGGCCGA GGAGCTCAAT GTCGAACGTC GGCGGATTTA TGACATCGTG 
AATGTACTAG AGAGCCTGCA CATGGTGAGC CGCCTCGCCA AAAACAGGTA CACTTGGCAC 
GGCCGGCACA ATCTCACCAA AACCCTGGGG ACGCTGAAGA GTGTCGGGGA AGAGAACAAG 
TACGCGGAGC AGATCATGAT GATCAAAAGG AAAGAGCATG AGCAAGAGTT CGACTTCATT 
AAGAGCTGTG GCCTAGAGGA CCACGTGATC AAGGGGGACC ACGTGATCAA GTCAACCGCT 
GGCCAGAACG GGCACTCAGA CATGTGCTTC GTAGAGCTCC CTGGAGTGGA ATTTCGGGCA 
GCTTCTGCAA ACAGCCGCAA AGACAAGTCC TTACGCGTGA TGAGCCAGAA GTTTGTGATG 
CTGTTCTTGG TGTCGACGCC TCAGATAGTA AGCCTGGAGA TCGCTGCCAA GATCTTAATT 
GGGGAGGACC ACGTGGAAGA TCTGGATAAA AGCAAGTTTA AAACAAAAAT TAGGAGGCTG 
TACGATATTG CTAATGTTCT GAGTAGCTTG GATCTTATCA AGAAAGTTCA TGTTACAGAA 
GAACGAGGAC GGAAGCCAGC TTTCAAATGG ACAGGCCCGG AAATCAGCCC AAATAACAGT 
GGCTCCAGCC CAGTCATGCC TCTCACCGCC TCCTTAGAGG CTGAGCAGTC TGCAAAAGAG 
AACTGTGCCA AAAACCTCTT TTCTACACGG GGGAAACCCA GCTTTACTCG ACACCCATCT 
CTTATCAAAT TGGTAAAGAG CATAGAAAAT GATCGGAGGA AGATCAGTTC CGCTCCCAGC 
AGCCCTGTCA AGAGCAGCAA AGCTGAGAGT TCTCAAAATT CTCCACCCGT CCCAAACAAA 
ATGGCCCAGC TCGCTGCTAT CTGCAAGATG CAGTTGGAGG AGCAGTCAAG TGAACCCAGG 
AAGAGAGTAA AAGTCAACCT AACAAGATCT GGGCACTACA AACCACTGGC TCCCCTGGAC 
CCTGCAGTGA ACACTGAGCT GGAACTGCTT GCTCCGTCCC TTATCCAGCC CCTGGGCATG 
GTCCCCCTGA TCCCTAGCCC ATTGTCATCT GCAGTGCCTG TGATCCTACC TCAGGCCCCT 
TCGGGCCCAT CCTATGCCAT CTACTTGCAA CCTGCCCAAG CCCAAATGTT GACACCACCC 
CATGGCCTGA GCCCAACAGT CTGCCCCACC CAGTCCTCTA ATGCTACCGG ATCCAAAGAC 
CCTACAGACG CCCCCACTGA GAAGACGGCC ACGGATGCCA CAAAGTCCAG TGCCTCCTGC 
AGACCCGGAA GCTTGCAGCC AGCACCAGAA AGGCAAGGTG CTAAGAATCG AAGCAAGGAG 
ACGACCGGAG ATCGAGGCAC GAAGAGGACG GGCGCCTTGG AGGACGGTGG TCCCGGTCCC 
ATAAAGAAAC CTAAAGAGGA CCTGAAGGCG CTTGAGAATG TCCCCACCCC CACGACCCTG 
TTCCCATCAG GATACCTAAT CCCTCTTACC CAGTGCCCAT CCCTGGGGCC AGACCCTATG 
TTGTCTAACA CAGAAAACTC AGGTACACTT TCCCCAAACC ACAGGATCTA CGGCTCCCCA 
ATTGCAGGTG TGATCCCAGT GGCGTCGTCT GAACTCACTG CTGTGAATTT TCCCCCATTC 
CATGTGACAC CGCTGAAACT TATGGTCTCC CCGACGTCTA TGGCAGCCGT ACCTGTTGGG 
AACAGCCCGG CTCTCAGCTC CAGCCACCCT GCTCCCACCC AGAACCCAAG CTCAGCCATT 
GTAAACTTTA CACTGCAGCA CTTGGGACTC ATCTCCCCCG GCGTGCAGAT GTCTGCCAGC 
CCCGGGCCTG GAGCTGGCAC AGTCCCTCTG TCCCCACGTG TAGAAGCAGC TAGCGTCATA 
CCAGATAACT TGAGCTCTCG GCAAGGGAGG GCCACCATCC ATGACTCTCC AGTCCTGGGC 
CAAAGCCAAC TAAATGGACA ACCAGTTGCT GGGACAGGGG CACAGCAGCC TGTTCCTGTG 
ACGCCCAAAG GCTCCCAGTT GGTGGCTGAA AGTTTCTTCC GTACTCCAGG TGGACCAACC 
AAGCCCACCA GCTCATCTTT CATGGATTTC GATGGTGCTA ACAAAACCTC CTTTGGAACC 
CTCTTTGTCC CACAGCGCAA ACTGGAAGTC TCAACTGAGG ATGTCCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094050.1
CDS	826..3456
Translation	MENQKENLFSEPHKRGLVKSPLQESSKANVVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSGLPEARDCLHEPQAKTNEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEHEQEFDFIKSCGLEDHVIKGDHVIKSTAGQNGHSDMCFVELPGVEFRAASANSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPVMPLTASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSSKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKRVKVNLTRSGHYKPLAPLDPAVNTELELLAPSLIQPLGMVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPHGLSPTVCPTQSSNATGSKDPTDAPTEKTATDATKSSASCRPGSLQPAPERQGAKNRSKETTGDRGTKRTGALEDGGPGPIKKPKEDLKALENVPTPTTLFPSGYLIPLTQCPSLGPDPMLSNTENSGTLSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALSSSHPAPTQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPLSPRVEAASVIPDNLSSRQGRATIHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSFMDFDGANKTSFGTLFVPQRKLEVSTEDVH

Target ORF information:

RefSeq Version	NM_001100580.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E2F transcription factor 8 (E2f8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100580.1

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GGTGAAAAGC 
CCCCTGCAAG AGTCCTCGAA GGCCAACGTG GTACTGGCTG AGATCCAGCC TGACCTGGGT 
CCTTTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTTAT CAGCGCGGTG AGCCCAGAGA TCCGAAGCCG AGATCAGAAA 
AGGGGCCTGT CCGACAACCG CAGTGGATTA CCCGAAGCTA GAGACTGTTT GCATGAACCA 
CAAGCGAAGA CGAACGAGAA GTCCCAGCCA AGTCGGAAGG AGAAGAGCTT GGGGTTGCTA 
TGCCACAAAT TCTTAGCGAG GTACCCTAAG TACCCCAACC CCGCTGTGAA TAACGACATC 
TGCCTGGACG AGGTGGCCGA GGAGCTCAAT GTCGAACGTC GGCGGATTTA TGACATCGTG 
AATGTACTAG AGAGCCTGCA CATGGTGAGC CGCCTCGCCA AAAACAGGTA CACTTGGCAC 
GGCCGGCACA ATCTCACCAA AACCCTGGGG ACGCTGAAGA GTGTCGGGGA AGAGAACAAG 
TACGCGGAGC AGATCATGAT GATCAAAAGG AAAGAGCATG AGCAAGAGTT CGACTTCATT 
AAGAGCTGTG GCCTAGAGGA CCACGTGATC AAGGGGGACC ACGTGATCAA GTCAACCGCT 
GGCCAGAACG GGCACTCAGA CATGTGCTTC GTAGAGCTCC CTGGAGTGGA ATTTCGGGCA 
GCTTCTGCAA ACAGCCGCAA AGACAAGTCC TTACGCGTGA TGAGCCAGAA GTTTGTGATG 
CTGTTCTTGG TGTCGACGCC TCAGATAGTA AGCCTGGAGA TCGCTGCCAA GATCTTAATT 
GGGGAGGACC ACGTGGAAGA TCTGGATAAA AGCAAGTTTA AAACAAAAAT TAGGAGGCTG 
TACGATATTG CTAATGTTCT GAGTAGCTTG GATCTTATCA AGAAAGTTCA TGTTACAGAA 
GAACGAGGAC GGAAGCCAGC TTTCAAATGG ACAGGCCCGG AAATCAGCCC AAATAACAGT 
GGCTCCAGCC CAGTCATGCC TCTCACCGCC TCCTTAGAGG CTGAGCAGTC TGCAAAAGAG 
AACTGTGCCA AAAACCTCTT TTCTACACGG GGGAAACCCA GCTTTACTCG ACACCCATCT 
CTTATCAAAT TGGTAAAGAG CATAGAAAAT GATCGGAGGA AGATCAGTTC CGCTCCCAGC 
AGCCCTGTCA AGAGCAGCAA AGCTGAGAGT TCTCAAAATT CTCCACCCGT CCCAAACAAA 
ATGGCCCAGC TCGCTGCTAT CTGCAAGATG CAGTTGGAGG AGCAGTCAAG TGAACCCAGG 
AAGAGAGTAA AAGTCAACCT AACAAGATCT GGGCACTACA AACCACTGGC TCCCCTGGAC 
CCTGCAGTGA ACACTGAGCT GGAACTGCTT GCTCCGTCCC TTATCCAGCC CCTGGGCATG 
GTCCCCCTGA TCCCTAGCCC ATTGTCATCT GCAGTGCCTG TGATCCTACC TCAGGCCCCT 
TCGGGCCCAT CCTATGCCAT CTACTTGCAA CCTGCCCAAG CCCAAATGTT GACACCACCC 
CATGGCCTGA GCCCAACAGT CTGCCCCACC CAGTCCTCTA ATGCTACCGG ATCCAAAGAC 
CCTACAGACG CCCCCACTGA GAAGACGGCC ACGGATGCCA CAAAGTCCAG TGCCTCCTGC 
AGACCCGGAA GCTTGCAGCC AGCACCAGAA AGGCAAGGTG CTAAGAATCG AAGCAAGGAG 
ACGACCGGAG ATCGAGGCAC GAAGAGGACG GGCGCCTTGG AGGACGGTGG TCCCGGTCCC 
ATAAAGAAAC CTAAAGAGGA CCTGAAGGCG CTTGAGAATG TCCCCACCCC CACGACCCTG 
TTCCCATCAG GATACCTAAT CCCTCTTACC CAGTGCCCAT CCCTGGGGCC AGACCCTATG 
TTGTCTAACA CAGAAAACTC AGGTACACTT TCCCCAAACC ACAGGATCTA CGGCTCCCCA 
ATTGCAGGTG TGATCCCAGT GGCGTCGTCT GAACTCACTG CTGTGAATTT TCCCCCATTC 
CATGTGACAC CGCTGAAACT TATGGTCTCC CCGACGTCTA TGGCAGCCGT ACCTGTTGGG 
AACAGCCCGG CTCTCAGCTC CAGCCACCCT GCTCCCACCC AGAACCCAAG CTCAGCCATT 
GTAAACTTTA CACTGCAGCA CTTGGGACTC ATCTCCCCCG GCGTGCAGAT GTCTGCCAGC 
CCCGGGCCTG GAGCTGGCAC AGTCCCTCTG TCCCCACGTG TAGAAGCAGC TAGCGTCATA 
CCAGATAACT TGAGCTCTCG GCAAGGGAGG GCCACCATCC ATGACTCTCC AGTCCTGGGC 
CAAAGCCAAC TAAATGGACA ACCAGTTGCT GGGACAGGGG CACAGCAGCC TGTTCCTGTG 
ACGCCCAAAG GCTCCCAGTT GGTGGCTGAA AGTTTCTTCC GTACTCCAGG TGGACCAACC 
AAGCCCACCA GCTCATCTTT CATGGATTTC GATGGTGCTA ACAAAACCTC CTTTGGAACC 
CTCTTTGTCC CACAGCGCAA ACTGGAAGTC TCAACTGAGG ATGTCCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E2F8 is essential for polyploidization in mammalian cells.
Nature cell biology14(11)1181-91(2012 Nov)
Pandit SK,Westendorp B,Nantasanti S,van Liere E,Tooten PC,Cornelissen PW,Toussaint MJ,Lamers WH,de Bruin A

Canonical and atypical E2Fs regulate the mammalian endocycle.
Nature cell biology14(11)1192-202(2012 Nov)
Chen HZ,Ouseph MM,Li J,P?cot T,Chokshi V,Kent L,Bae S,Byrne M,Duran C,Comstock G,Trikha P,Mair M,Senapati S,Martin CK,Gandhi S,Wilson N,Liu B,Huang YW,Thompson JC,Raman S,Singh S,Leone M,Machiraju R,Huang K,Mo X,Fernandez S,Kalaszczynska I,Wolgemuth DJ,Sicinski P,Huang T,Jin V,Leone G

E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1.
The EMBO journal31(19)3871-84(2012 Oct)
Weijts BG,Bakker WJ,Cornelissen PW,Liang KH,Schaftenaar FH,Westendorp B,de Wolf CA,Paciejewska M,Scheele CL,Kent L,Leone G,Schulte-Merker S,de Bruin A

Atypical E2F repressors and activators coordinate placental development.
Developmental cell22(4)849-62(2012 Apr)
Ouseph MM,Li J,Chen HZ,P?cot T,Wenzel P,Thompson JC,Comstock G,Chokshi V,Byrne M,Forde B,Chong JL,Huang K,Machiraju R,de Bruin A,Leone G

Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription.
Nucleic acids research33(17)5458-70(2005)
Christensen J,Cloos P,Toftegaard U,Klinkenberg D,Bracken AP,Trinh E,Heeran M,Di Stefano L,Helin K

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E2f8 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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