Pou3f4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pou3f4
Entrez Gene ID	29589
Full Name	POU class 3 homeobox 4
Synonyms	RHS2
General protein information	Preferred Names POU class 3 homeobox 4 Names POU domain, class 3, transcription factor 4 OTF-9 RHS2 class III POU protein brain-4 brain-specific homeobox/POU domain protein 4 brn-4 oct-9 octamer-binding protein 9 octamer-binding transcription factor 9
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq31
Summary	binds octamer motif containing DNA sequences; acts as a transcriptional transactivator; may play a role in early neural development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017252.2	NP_058948.1	POU domain, class 3, transcription factor 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	POU3F4	XP_003317585.1
	POU3F4	XP_001104059.1
Canis lupus familiaris (dog)	POU3F4	XP_549108.1
Mus musculus (house mouse)	Pou3f4	NP_032927.1
Rattus norvegicus (Norway rat)	Pou3f4	NP_058948.1
Homo sapiens (human)	POU3F4	NP_000298.3
Bos taurus (cattle)	POU3F4	XP_002699986.1
Xenopus tropicalis (tropical clawed frog)	pou3f4	NP_001090728.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea.
Uetsuka S, Ogata G, Nagamori S, Isozumi N, Nin F, Yoshida T, Komune S, Kitahara T, Kikkawa Y, Inohara H, Kanai Y, Hibino H
The European journal of neuroscience42(3)1984-2002(2015 Aug)

The co-transduction of Nurr1 and Brn4 genes induces the differentiation of neural stem cells into dopaminergic neurons.
Tan XF, Jin GH, Tian ML, Qin JB, Zhang L, Zhu HX, Li HM
Cell biology international35(12)1217-23(2011 Dec)

The role of Brn-4 in the regulation of neural stem cell differentiation into neurons.
Shi J, Jin G, Zhu H, Tian M, Zhang X, Qin J, Tan X
Neuroscience research67(1)8-17(2010 May)

Brn-4 is upregulated in the deafferented hippocampus and promotes neuronal differentiation of neural progenitors in vitro.
Zhang X, Jin G, Wang L, Hu W, Tian M, Qin J, Huang H
Hippocampus19(2)176-86(2009 Feb)

Cooperative function of Tbx1 and Brn4 in the periotic mesenchyme is necessary for cochlea formation.
Braunstein EM, Crenshaw EB 3rd, Morrow BE, Adams JC
Journal of the Association for Research in Otolaryngology : JARO9(1)33-43(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The co-transduction of Nurr1 and Brn4 genes induces cell differentiation of neural stem cells
Title: The co-transduction of Nurr1 and Brn4 genes induces the differentiation of neural stem cells into dopaminergic neurons.

Brn-4 expression is required both for neuronal differentiation of NSCs and maturation of newborn neurons, and that there may be some regulatory factors in deafferented hippocampus that can regulate Brn-4 expression in neuronal progenitors.
Title: The role of Brn-4 in the regulation of neural stem cell differentiation into neurons.

study showed after fimbria-fornix lesion, Brn-4 in deafferented hippocampus was upregulated & might play a role in inducing local progenitors to differentiate into neurons, which may compensate for loss of cholinergic afferent fibers or other dysfunctions
Title: Brn-4 is upregulated in the deafferented hippocampus and promotes neuronal differentiation of neural progenitors in vitro.

The following Pou3f4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pou3f4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42555	NM_017252.2 Latest version!	Rattus norvegicus POU class 3 homeobox 4 (Pou3f4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pou3f4 ( NM_017252.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058948.1 Rattus norvegicus POU class 3 homeobox 4 (Pou3f4), mRNA.

Price & Availability↑

CloneID	ORa42555
Clone ID Related Accession (Same CDS sequence)	NM_017252.2
Accession Version	NM_017252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	POU domain, class 3, transcription factor 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M84645.1 and Z11834.1. On Nov 23, 2005 this sequence version replaced NM_017252.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCCACAG CTGCCTCGAA TCCCTACAGC ATTCTCAGTT CCAGCTCCCT CGTCCATGCG 
GACTCTGCGG GCATGCAGCA GGGAAGTCCT TTCCGCAATC CTCAGAAACT TCTCCAAAGT 
GACTACTTGC AGGGAGTTCC CAGCAATGGG CATCCCCTCG GGCATCACTG GGTGACCAGT 
CTGAGCGACG GGGGCCCGTG GTCCTCCACA TTGGCCACCA GCCCCCTGGA CCAGCAGGAC 
GTGAAGCCGG GACGTGAAGA TCTGCAACTG GGAGCAATCA TCCATCACCG CTCGCCTCAC 
GTAGCCCACC ACTCGCCCCA CACTAACCAT CCGAACGCCT GGGGAGCGAG CCCGGCTCCA 
AACTCGTCCA TCACGTCCAG CGGCCAACCT CTCAATGTGT ACTCGCAGCC AGGCTTCACG 
GTGAGTGGTA TGCTAGAGCA CGGGGGACTC ACTCCACCAC CAGCTGCTGC CTCCACACAG 
AGCCTGCATC CAGTGCTCCG GGAGCCTCCA GACCATGGTG AGCTGGGCTC GCACCACTGC 
CAGGACCACT CGGATGAAGA GACTCCAACC TCTGATGAGT TGGAACAGTT CGCCAAACAA 
TTCAAACAAA GAAGAATCAA GTTGGGTTTC ACGCAAGCCG ACGTGGGGCT GGCATTGGGC 
ACACTGTATG GCAACGTGTT CTCGCAGACT ACCATCTGCA GGTTCGAGGC CTTGCAACTG 
AGCTTCAAGA ACATGTGCAA GCTGAAACCA CTGCTAAATA AGTGGCTGGA GGAGGCTGAT 
TCATCCACAG GAAGCCCGAC CAGCATTGAC AAGATCGCCG CTCAGGGCCG TAAACGCAAG 
AAGCGAACCT CCATTGAGGT GAGTGTCAAG GGCGTACTGG AAACGCATTT CCTCAAGTGT 
CCCAAGCCTG CAGCACAGGA GATCTCCTCG CTGGCAGACA GTCTCCAGTT GGAGAAAGAA 
GTGGTACGTG TCTGGTTCTG TAATAGAAGA CAAAAAGAAA AAAGAATGAC TCCGCCAGGG 
GATCAGCAGC CACACGAGGT TTATTCGCAC ACGGTGAAAA CAGATGCGTC CTGCCACGAT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058948.1
CDS	33..1118
Translation	MATAASNPYSILSSSSLVHADSAGMQQGSPFRNPQKLLQSDYLQGVPSNGHPLGHHWVTSLSDGGPWSSTLATSPLDQQDVKPGREDLQLGAIIHHRSPHVAHHSPHTNHPNAWGASPAPNSSITSSGQPLNVYSQPGFTVSGMLEHGGLTPPPAAASTQSLHPVLREPPDHGELGSHHCQDHSDEETPTSDELEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSTGSPTSIDKIAAQGRKRKKRTSIEVSVKGVLETHFLKCPKPAAQEISSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGDQQPHEVYSHTVKTDASCHDL

Target ORF information:

RefSeq Version	NM_017252.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus POU class 3 homeobox 4 (Pou3f4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017252.2

ORF Insert Sequence:

ATGGCCACAG CTGCCTCGAA TCCCTACAGC ATTCTCAGTT CCAGCTCCCT CGTCCATGCG 
GACTCTGCGG GCATGCAGCA GGGAAGTCCT TTCCGCAATC CTCAGAAACT TCTCCAAAGT 
GACTACTTGC AGGGAGTTCC CAGCAATGGG CATCCCCTCG GGCATCACTG GGTGACCAGT 
CTGAGCGACG GGGGCCCGTG GTCCTCCACA TTGGCCACCA GCCCCCTGGA CCAGCAGGAC 
GTGAAGCCGG GACGTGAAGA TCTGCAACTG GGAGCAATCA TCCATCACCG CTCGCCTCAC 
GTAGCCCACC ACTCGCCCCA CACTAACCAT CCGAACGCCT GGGGAGCGAG CCCGGCTCCA 
AACTCGTCCA TCACGTCCAG CGGCCAACCT CTCAATGTGT ACTCGCAGCC AGGCTTCACG 
GTGAGTGGTA TGCTAGAGCA CGGGGGACTC ACTCCACCAC CAGCTGCTGC CTCCACACAG 
AGCCTGCATC CAGTGCTCCG GGAGCCTCCA GACCATGGTG AGCTGGGCTC GCACCACTGC 
CAGGACCACT CGGATGAAGA GACTCCAACC TCTGATGAGT TGGAACAGTT CGCCAAACAA 
TTCAAACAAA GAAGAATCAA GTTGGGTTTC ACGCAAGCCG ACGTGGGGCT GGCATTGGGC 
ACACTGTATG GCAACGTGTT CTCGCAGACT ACCATCTGCA GGTTCGAGGC CTTGCAACTG 
AGCTTCAAGA ACATGTGCAA GCTGAAACCA CTGCTAAATA AGTGGCTGGA GGAGGCTGAT 
TCATCCACAG GAAGCCCGAC CAGCATTGAC AAGATCGCCG CTCAGGGCCG TAAACGCAAG 
AAGCGAACCT CCATTGAGGT GAGTGTCAAG GGCGTACTGG AAACGCATTT CCTCAAGTGT 
CCCAAGCCTG CAGCACAGGA GATCTCCTCG CTGGCAGACA GTCTCCAGTT GGAGAAAGAA 
GTGGTACGTG TCTGGTTCTG TAATAGAAGA CAAAAAGAAA AAAGAATGAC TCCGCCAGGG 
GATCAGCAGC CACACGAGGT TTATTCGCAC ACGGTGAAAA CAGATGCGTC CTGCCACGAT 
CTCTGA