Por cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Por
Entrez Gene ID	29441
Full Name	cytochrome p450 oxidoreductase
Synonyms	CYPOR
General protein information	Preferred Names cytochrome p450 oxidoreductase Names NADPH--cytochrome P450 reductase CPR P450 (cytochrome) oxidoreductase P450R cytochrome P450 reductase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	plays a role in electron transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031576.1	NP_113764.1	NADPH--cytochrome P450 reductase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	POR	NP_001171276.1
Gallus gallus (chicken)	POR	NP_001182725.1
Kluyveromyces lactis	KLLA0D08712g	XP_453451.1
Homo sapiens (human)	POR	NP_000932.3
Pan troglodytes (chimpanzee)	POR	XP_001155755.2
	POR	XP_001109664.1
Drosophila melanogaster (fruit fly)	Cpr	NP_001260128.1
Caenorhabditis elegans (roundworm)	emb-8	NP_498103.1
Oryza sativa (rice)	Os09g0558900	NP_001063914.1
Oryza sativa (rice)	Os08g0243500	NP_001061346.1
Rattus norvegicus (Norway rat)	Por	NP_113764.1
Eremothecium gossypii	AGOS_AAL121C	NP_982421.1
Schizosaccharomyces pombe (fission yeast)	ccr1	NP_596046.1
Magnaporthe oryzae (rice blast fungus)	MGG_07117	XP_003715306.1
Danio rerio (zebrafish)	por	NP_001030154.1
Bos taurus (cattle)	POR	NP_001030467.1
Mus musculus (house mouse)	Por	NP_032924.1
Saccharomyces cerevisiae (baker's yeast)	NCP1	NP_011908.1
Neurospora crassa	NCU09741	XP_964443.1
Arabidopsis thaliana (thale cress)	ATR2	NP_849472.2
Xenopus tropicalis (tropical clawed frog)	por	XP_002933864.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural and kinetic studies of Asp632 mutants and fully-reduced NADPH-cytochrome P450 oxidoreductase define the role of Asp632 loop dynamics in control of NADPH binding and hydride transfer.
Xia C, Rwere F, Im S, Shen A, Waskell LA, Kim JP
Biochemistry(2018 Jan)

Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1.
Gonzalez E, Guengerich FP
The Journal of biological chemistry292(32)13168-13185(2017 08)

Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or Gly-143 Destabilize Its FMN Semiquinone.
Rwere F, Xia C, Im S, Haque MM, Stuehr DJ, Waskell L, Kim JJ
The Journal of biological chemistry291(28)14639-61(2016 Jul)

Kinetic and structural characterization of the interaction between the FMN binding domain of cytochrome P450 reductase and cytochrome c.
Huang R, Zhang M, Rwere F, Waskell L, Ramamoorthy A
The Journal of biological chemistry290(8)4843-55(2015 Feb)

CYP2J2 epoxygenase membrane anchor plays an important role in facilitating electron transfer from CPR.
Meling DD, McDougle DR, Das A
Journal of inorganic biochemistry14247-53(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest CYP17A1, with electron donor NADPH-P450 reductase, is inherently distributive enzyme but that some processivity is present; some 17alpha-hydroxy pregnenolone formed does not dissociate from CYP17A1 before conversion to dehydroepiandrosterone; CYB5A does not enhance reaction by decreasing k(off) of ligands of CYP17A1. (CYP17A1 = cytochrome P450, family 17, subfamily A, polypeptide 1; CYB5A = cytochrome b5)
Title: Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1.

The DeltaGly-143 protein had moderately decreased activity with cytochrome P450 and cytochrome c. It formed a flexible loop, which transiently interacts with the flavin N5, resulting in the generation of both an unstable neutral blue semiquinone and hydroquinone.
Title: Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or Gly-143 Destabilize Its FMN Semiquinone.

The findings provide insights into the function of CPR and CPR-cyt c interaction on a structural basis.
Title: Kinetic and structural characterization of the interaction between the FMN binding domain of cytochrome P450 reductase and cytochrome c.

the N-terminus of CYP2J2 is very important to form a productive CYP-CPR complex to facilitate electron transfer.
Title: CYP2J2 epoxygenase membrane anchor plays an important role in facilitating electron transfer from CPR.

the structure and dynamics of the full-length NADPH-cytochrome P450 oxidoreductase
Title: Probing the transmembrane structure and dynamics of microsomal NADPH-cytochrome P450 oxidoreductase by solid-state NMR.

The following Por gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Por cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08145	NM_031576.1 Latest version!	Rattus norvegicus cytochrome p450 oxidoreductase (Por), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$398.30 $569.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Por ( NM_031576.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113764.1 Rattus norvegicus cytochrome p450 oxidoreductase (Por), mRNA.

Price & Availability↑

CloneID	ORa08145
Clone ID Related Accession (Same CDS sequence)	NM_031576.1
Accession Version	NM_031576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	NADPH--cytochrome P450 reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M12516.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M12516.1, M10068.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGACT CTCACGAAGA CACCAGTGCC ACCATGCCTG AGGCCGTGGC TGAAGAAGTG 
TCTCTATTCA GCACGACGGA CATGGTTCTG TTTTCTCTCA TCGTGGGGGT CCTGACCTAC 
TGGTTCATCT TTAGAAAGAA GAAAGAAGAG ATACCGGAGT TCAGCAAGAT CCAAACAACG 
GCCCCACCCG TCAAAGAGAG CAGCTTCGTG GAAAAGATGA AGAAAACGGG AAGGAACATT 
ATCGTATTCT ATGGCTCCCA GACGGGAACC GCTGAGGAGT TTGCCAACCG GCTGTCCAAG 
GATGCCCACC GCTACGGGAT GCGGGGCATG TCCGCAGACC CTGAAGAGTA TGACTTGGCC 
GACCTGAGCA GCCTGCCTGA GATCGACAAG TCCCTGGTAG TCTTCTGCAT GGCCACATAC 
GGAGAGGGCG ACCCCACGGA CAATGCGCAG GACTTCTATG ACTGGCTGCA GGAGACTGAC 
GTGGACCTCA CTGGGGTCAA GTTTGCTGTA TTTGGTCTTG GGAACAAGAC CTATGAGCAC 
TTCAATGCCA TGGGCAAGTA TGTGGACCAG CGGCTGGAGC AGCTTGGCGC CCAGCGCATC 
TTTGAGTTGG GCCTTGGTGA TGATGACGGG AACTTGGAAG AGGATTTCAT CACGTGGAGG 
GAGCAGTTCT GGCCAGCTGT GTGCGAGTTC TTTGGGGTAG AAGCCACTGG GGAGGAGTCG 
AGCATTCGCC AGTATGAGCT CGTGGTCCAC GAAGACATGG ACGTAGCCAA GGTGTACACG 
GGTGAGATGG GCCGTCTGAA GAGCTACGAG AACCAGAAAC CCCCCTTCGA TGCTAAGAAT 
CCATTCCTGG CTGCTGTCAC CGCCAACCGG AAGCTGAACC AAGGCACTGA GCGGCATCTA 
ATGCACCTGG AGTTGGACAT CTCAGACTCC AAGATCAGGT ATGAATCTGG AGATCACGTG 
GCTGTGTACC CAGCCAATGA CTCAGCCCTG GTCAACCAGA TTGGGGAGAT CCTGGGAGCT 
GACCTGGATG TCATCATGTC TCTAAACAAT CTCGATGAGG AGTCAAACAA GAAGCATCCG 
TTCCCCTGCC CCACCACCTA CCGCACGGCC CTCACCTACT ACCTGGACAT CACTAACCCG 
CCACGCACCA ATGTGCTCTA CGAACTGGCA CAGTACGCCT CAGAGCCCTC GGAGCAGGAG 
CACCTGCACA AGATGGCGTC ATCCTCAGGC GAGGGCAAGG AGCTGTACCT GAGCTGGGTG 
GTGGAAGCCC GGAGGCACAT CCTAGCCATC CTCCAAGACT ACCCATCACT GCGGCCACCC 
ATCGACCACC TGTGTGAGCT GCTGCCACGC CTGCAGGCCC GATACTACTC CATTGCCTCA 
TCCTCCAAGG TCCACCCCAA CTCCGTGCAC ATCTGTGCCG TGGCCGTGGA GTACGAAGCG 
AAGTCTGGCC GAGTGAACAA GGGGGTGGCC ACTAGCTGGC TTCGGGCCAA GGAACCAGCA 
GGCGAGAATG GCGGCCGCGC CCTGGTACCC ATGTTCGTGC GCAAATCTCA GTTCCGCTTG 
CCTTTCAAGT CCACCACACC TGTCATCATG GTGGGCCCCG GCACTGGGAT TGCCCCTTTC 
ATGGGCTTCA TCCAGGAACG AGCTTGGCTT CGAGAGCAAG GCAAGGAGGT GGGAGAGACG 
CTGCTATACT ATGGCTGCCG GCGCTCGGAT GAGGACTATC TGTACCGTGA AGAGCTAGCC 
CGCTTCCACA AGGACGGTGC CCTCACGCAG CTTAATGTGG CCTTTTCCCG GGAGCAGGCC 
CACAAGGTCT ATGTCCAGCA CCTTCTGAAG AGAGACAGGG AACACCTGTG GAAGCTGATC 
CACGAGGGCG GTGCCCACAT CTATGTGTGC GGGGATGCTC GAAATATGGC CAAAGATGTG 
CAAAACACAT TCTATGACAT TGTGGCTGAG TTCGGGCCCA TGGAGCACAC CCAGGCTGTG 
GACTATGTTA AGAAGCTGAT GACCAAGGGC CGCTACTCAC TAGATGTGTG GAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113764.1
CDS	41..2077
Translation	MGDSHEDTSATMPEAVAEEVSLFSTTDMVLFSLIVGVLTYWFIFRKKKEEIPEFSKIQTTAPPVKESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDKSLVVFCMATYGEGDPTDNAQDFYDWLQETDVDLTGVKFAVFGLGNKTYEHFNAMGKYVDQRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEFFGVEATGEESSIRQYELVVHEDMDVAKVYTGEMGRLKSYENQKPPFDAKNPFLAAVTANRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQIGEILGADLDVIMSLNNLDEESNKKHPFPCPTTYRTALTYYLDITNPPRTNVLYELAQYASEPSEQEHLHKMASSSGEGKELYLSWVVEARRHILAILQDYPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVAVEYEAKSGRVNKGVATSWLRAKEPAGENGGRALVPMFVRKSQFRLPFKSTTPVIMVGPGTGIAPFMGFIQERAWLREQGKEVGETLLYYGCRRSDEDYLYREELARFHKDGALTQLNVAFSREQAHKVYVQHLLKRDREHLWKLIHEGGAHIYVCGDARNMAKDVQNTFYDIVAEFGPMEHTQAVDYVKKLMTKGRYSLDVWS

Target ORF information:

RefSeq Version	NM_031576.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome p450 oxidoreductase (Por), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031576.1

ORF Insert Sequence:

ATGGGGGACT CTCACGAAGA CACCAGTGCC ACCATGCCTG AGGCCGTGGC TGAAGAAGTG 
TCTCTATTCA GCACGACGGA CATGGTTCTG TTTTCTCTCA TCGTGGGGGT CCTGACCTAC 
TGGTTCATCT TTAGAAAGAA GAAAGAAGAG ATACCGGAGT TCAGCAAGAT CCAAACAACG 
GCCCCACCCG TCAAAGAGAG CAGCTTCGTG GAAAAGATGA AGAAAACGGG AAGGAACATT 
ATCGTATTCT ATGGCTCCCA GACGGGAACC GCTGAGGAGT TTGCCAACCG GCTGTCCAAG 
GATGCCCACC GCTACGGGAT GCGGGGCATG TCCGCAGACC CTGAAGAGTA TGACTTGGCC 
GACCTGAGCA GCCTGCCTGA GATCGACAAG TCCCTGGTAG TCTTCTGCAT GGCCACATAC 
GGAGAGGGCG ACCCCACGGA CAATGCGCAG GACTTCTATG ACTGGCTGCA GGAGACTGAC 
GTGGACCTCA CTGGGGTCAA GTTTGCTGTA TTTGGTCTTG GGAACAAGAC CTATGAGCAC 
TTCAATGCCA TGGGCAAGTA TGTGGACCAG CGGCTGGAGC AGCTTGGCGC CCAGCGCATC 
TTTGAGTTGG GCCTTGGTGA TGATGACGGG AACTTGGAAG AGGATTTCAT CACGTGGAGG 
GAGCAGTTCT GGCCAGCTGT GTGCGAGTTC TTTGGGGTAG AAGCCACTGG GGAGGAGTCG 
AGCATTCGCC AGTATGAGCT CGTGGTCCAC GAAGACATGG ACGTAGCCAA GGTGTACACG 
GGTGAGATGG GCCGTCTGAA GAGCTACGAG AACCAGAAAC CCCCCTTCGA TGCTAAGAAT 
CCATTCCTGG CTGCTGTCAC CGCCAACCGG AAGCTGAACC AAGGCACTGA GCGGCATCTA 
ATGCACCTGG AGTTGGACAT CTCAGACTCC AAGATCAGGT ATGAATCTGG AGATCACGTG 
GCTGTGTACC CAGCCAATGA CTCAGCCCTG GTCAACCAGA TTGGGGAGAT CCTGGGAGCT 
GACCTGGATG TCATCATGTC TCTAAACAAT CTCGATGAGG AGTCAAACAA GAAGCATCCG 
TTCCCCTGCC CCACCACCTA CCGCACGGCC CTCACCTACT ACCTGGACAT CACTAACCCG 
CCACGCACCA ATGTGCTCTA CGAACTGGCA CAGTACGCCT CAGAGCCCTC GGAGCAGGAG 
CACCTGCACA AGATGGCGTC ATCCTCAGGC GAGGGCAAGG AGCTGTACCT GAGCTGGGTG 
GTGGAAGCCC GGAGGCACAT CCTAGCCATC CTCCAAGACT ACCCATCACT GCGGCCACCC 
ATCGACCACC TGTGTGAGCT GCTGCCACGC CTGCAGGCCC GATACTACTC CATTGCCTCA 
TCCTCCAAGG TCCACCCCAA CTCCGTGCAC ATCTGTGCCG TGGCCGTGGA GTACGAAGCG 
AAGTCTGGCC GAGTGAACAA GGGGGTGGCC ACTAGCTGGC TTCGGGCCAA GGAACCAGCA 
GGCGAGAATG GCGGCCGCGC CCTGGTACCC ATGTTCGTGC GCAAATCTCA GTTCCGCTTG 
CCTTTCAAGT CCACCACACC TGTCATCATG GTGGGCCCCG GCACTGGGAT TGCCCCTTTC 
ATGGGCTTCA TCCAGGAACG AGCTTGGCTT CGAGAGCAAG GCAAGGAGGT GGGAGAGACG 
CTGCTATACT ATGGCTGCCG GCGCTCGGAT GAGGACTATC TGTACCGTGA AGAGCTAGCC 
CGCTTCCACA AGGACGGTGC CCTCACGCAG CTTAATGTGG CCTTTTCCCG GGAGCAGGCC 
CACAAGGTCT ATGTCCAGCA CCTTCTGAAG AGAGACAGGG AACACCTGTG GAAGCTGATC 
CACGAGGGCG GTGCCCACAT CTATGTGTGC GGGGATGCTC GAAATATGGC CAAAGATGTG 
CAAAACACAT TCTATGACAT TGTGGCTGAG TTCGGGCCCA TGGAGCACAC CCAGGCTGTG 
GACTATGTTA AGAAGCTGAT GACCAAGGGC CGCTACTCAC TAGATGTGTG GAGCTAG