Tcirg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tcirg1
Entrez Gene ID	293650
Full Name	T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3
Synonyms	OC116,TIRC7
General protein information	Preferred Names T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 Names V-type proton ATPase 116 kDa subunit a ATPase, H+ transporting, lysosomal V0 protein a T cell immune response cDNA7 protein T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 osteoclastic proton pump v-ATPase 116-kDa v-H+ATPase subunit a3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	mouse homolog is the a3 subunit of the plasma membrane V-ATPase essential for bone resorption [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006230727.2	XP_006230789.1	V-type proton ATPase 116 kDa subunit a isoform X1
XM_006230728.2	XP_006230790.1	V-type proton ATPase 116 kDa subunit a isoform X2
XM_008760097.2	XP_008758319.1	V-type proton ATPase 116 kDa subunit a isoform X1
NM_199089.2	NP_954520.2	V-type proton ATPase 116 kDa subunit a

Pathways from BioSystems

Homologies

Homo sapiens (human)	TCIRG1	NP_006010.2
Pan troglodytes (chimpanzee)	TCIRG1	XP_522295.3
Danio rerio (zebrafish)	zgc:55891	NP_998234.1
Danio rerio (zebrafish)	LOC100147923	XP_001922910.4
Xenopus tropicalis (tropical clawed frog)	tcirg1	NP_001016849.1
	TCIRG1	XP_001103418.2
Rattus norvegicus (Norway rat)	Tcirg1	NP_954520.2
Canis lupus familiaris (dog)	TCIRG1	XP_540812.1
Bos taurus (cattle)	TCIRG1	NP_001069102.1
Gallus gallus (chicken)	TCIRG1	NP_990053.1
Danio rerio (zebrafish)	tcirg1	XP_005160329.1
Mus musculus (house mouse)	Tcirg1	NP_001129563.1

Gene Ontology
Bibliography

Related articles in PubMed

Autophagy plays a critical role in the degradation of active RHOA, the control of cell cytokinesis, and genomic stability.
Belaid A, Cerezo M, Chargui A, Corcelle-Termeau E, Pedeutour F, Giuliano S, Ilie M, Rubera I, Tauc M, Barale S, Bertolotto C, Brest P, Vouret-Craviari V, Klionsky DJ, Carle GF, Hofman P, Mograbi B
Cancer research73(14)4311-22(2013 Jul)

RNA interference-mediated silencing of Atp6i prevents both periapical bone erosion and inflammation in the mouse model of endodontic disease.
Ma J, Chen W, Zhang L, Tucker B, Zhu G, Sasaki H, Hao L, Wang L, Ci H, Jiang H, Stashenko P, Li YP
Infection and immunity81(4)1021-30(2013 Apr)

Osteopetrosis, osteopetrorickets and hypophosphatemic rickets differentially affect dentin and enamel mineralization.
Koehne T, Marshall RP, Jeschke A, Kahl-Nieke B, Schinke T, Amling M
Bone53(1)25-33(2013 Mar)

Resveratrol improves cardiomyopathy in dystrophin-deficient mice through SIRT1 protein-mediated modulation of p300 protein.
Kuno A, Hori YS, Hosoda R, Tanno M, Miura T, Shimamoto K, Horio Y
The Journal of biological chemistry288(8)5963-72(2013 Feb)

The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2.
Hosokawa H, Dip PV, Merkulova M, Bakulina A, Zhuang Z, Khatri A, Jian X, Keating SM, Bueler SA, Rubinstein JL, Randazzo PA, Ausiello DA, Gr?ber G, Marshansky V
The Journal of biological chemistry288(8)5896-913(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2
Title: The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2.

The following Tcirg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcirg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04864	NM_199089.2 Latest version!	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa23724	XM_006230727.2 Latest version!	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa23724	XM_008760097.2 Latest version!	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa23725	XM_006230728.2 Latest version!	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tcirg1 ( NM_199089.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954520.2 Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), mRNA.

Price & Availability↑

CloneID	ORa04864
Clone ID Related Accession (Same CDS sequence)	NM_199089.2
Accession Version	NM_199089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase 116 kDa subunit a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ286426.1. On Feb 5, 2006 this sequence version replaced NM_199089.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ286426.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTCCA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGCA 
TCTGCCTACA ACTGTGTGAG CCAGCTGGGT GAGCTGGGCC TCGTGGAGTT TAGAGACCTC 
AACGAGTCGG TGAGTGCCTT CCAGAGACGC TTCGTGGTAG AGGTCCGGCG CTGCGAGGAG 
CTGGAGAAGA CCTTCACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGCCT GACACTGACC 
CCACCCGAGG GGACACTGCC AGCACCGCCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGACCGCT TGGCCCAGGA GCTTCGGGAT GTTCGAGGCA ACCAACAGGC ACTTCGGGCC 
CAGCTGCACC AGCTACAGCT CCACTCAGCC GTGCTGGGCC AGAGCCACGC CCCTCCGATG 
GCAGCTGTCC ATACGGAGGG ACCCACCACT GAGAGAACAC CCCTGCTTTC AGCCACCCCA 
GGGCCACATG CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC TTGCAAGGCT 
GCAGCCCTGG AGCGCCTGCT TTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTAAG 
GAGACTGAGG GGCAGCTGGA GGACCCGGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGGTTC GGAAGATCAC AGACTGCTTT 
CACTGTCATG TCTTCCCGTA CCTGGAGCAG GAGGAAGCCC GGCTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AGAGCCAGGA GCTTCAGGAG GTCCTGGGGG AGACAGAACG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCAGGGC AGGTGCAGAT CCGGAAGATG 
AAGGCCGTGC ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATT 
GCGGAGGTCT GGTGTGCCAC GAGGGACCTT CCCACTGTAC AGCAGGCGCT GCAGAGCGGC 
TCGAGTGAGG AAGGAGTGAG TGCTGTGGCT CACCGTATCC CTTGCCAGGA CATGCCTCCA 
ACTCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGAT ACAGGGAAGT CAACCCTGCT CCCTACACCA TCATCACATT CCCCTTCCTC 
TTTGCTGTGA TGTTTGGCGA CGTGGGCCAC GGACTGCTCA TGTTTCTCTT CGCCCTGGCT 
ATGGTCCTCA CTGAGAACCA ACCAGCTGTA AAGACCACGC AAAATGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGCTACCT ACCCCTGCTC ATGGGTCTGT TCTCCATCTA CACGGGCTTC 
ATCTACAACG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGCTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACCC CATGCTTACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTC GGACCTTATC CCTTCGGCAT TGACCCGATC 
TGGAGCCTGG CCACCAACCA CCTGAGCTTC CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACACACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCATGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCGTGCCTG AGCTCATCTT CCTGTTGGGC 
CTCTTTGGCT ACCTTGTTTT CCTCATTGTC TACAAGTGGC TCTACGTCTC AGCTGCCAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCCCAAAAC 
CCCACCAATC GGCTGCTCTT CCACGGGCAG GTAGTGGTAC AGTATGCGCT GGTGGTCCTG 
GCTTTGGCCA CAGTTCCTAT CCTGCTGCTG GGCACACCCT TGTACCTTCT GCGCCAGCAC 
CACCGAAGAA ACACTCAGAG AAGGCCTACA GCAGGCAGAC AGGATGAGGA CAGTGACAAG 
CTTCTGGCCT CCCCGGATGC TTCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCT CAGGAGATGA AGAAGCTGAG TTTGTCCCTT CGGAGATCTT CATGCACCAA 
GCCATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC TCATGCTCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TAGGCATGGG CCGAGAGATT GGTGTGGCAT CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCTTGCTAG TGATGGAGGG GCTCTCAGCC 
TTCCTACATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGGACC 
GGCTACAAGC TCAGCCCCTT CGCCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954520.2
CDS	1..2505
Translation	MGSMFRSEEVALVQLLLPTASAYNCVSQLGELGLVEFRDLNESVSAFQRRFVVEVRRCEELEKTFTFLREEVQRAGLTLTPPEGTLPAPPPRDLLRIQEETDRLAQELRDVRGNQQALRAQLHQLQLHSAVLGQSHAPPMAAVHTEGPTTERTPLLSATPGPHADLKVNFVAGAVEPCKAAALERLLWRACRGFLIASFKETEGQLEDPVTGEPATWMTFVISYWGEQIGQKVRKITDCFHCHVFPYLEQEEARLRTLQQLQQQSQELQEVLGETERFLSQVLGRVQQLLPPGQVQIRKMKAVHLTLNQCSVNTTHKCLIAEVWCATRDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENQPAVKTTQNEIWQTFFGGRYLPLLMGLFSIYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHPMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETVPELIFLLGLFGYLVFLIVYKWLYVSAASASSAPSILIHFINMFLFSQNPTNRLLFHGQVVVQYALVVLALATVPILLLGTPLYLLRQHHRRNTQRRPTAGRQDEDSDKLLASPDASTLENSWSPDEEKAGSSGDEEAEFVPSEIFMHQAIHTIEFCLGCISNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGMGREIGVASVVLVPVFAAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFAFTVDSD

Target ORF information:

RefSeq Version	NM_199089.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199089.2

ORF Insert Sequence:

ATGGGCTCCA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGCA 
TCTGCCTACA ACTGTGTGAG CCAGCTGGGT GAGCTGGGCC TCGTGGAGTT TAGAGACCTC 
AACGAGTCGG TGAGTGCCTT CCAGAGACGC TTCGTGGTAG AGGTCCGGCG CTGCGAGGAG 
CTGGAGAAGA CCTTCACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGCCT GACACTGACC 
CCACCCGAGG GGACACTGCC AGCACCGCCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGACCGCT TGGCCCAGGA GCTTCGGGAT GTTCGAGGCA ACCAACAGGC ACTTCGGGCC 
CAGCTGCACC AGCTACAGCT CCACTCAGCC GTGCTGGGCC AGAGCCACGC CCCTCCGATG 
GCAGCTGTCC ATACGGAGGG ACCCACCACT GAGAGAACAC CCCTGCTTTC AGCCACCCCA 
GGGCCACATG CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC TTGCAAGGCT 
GCAGCCCTGG AGCGCCTGCT TTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTAAG 
GAGACTGAGG GGCAGCTGGA GGACCCGGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGGTTC GGAAGATCAC AGACTGCTTT 
CACTGTCATG TCTTCCCGTA CCTGGAGCAG GAGGAAGCCC GGCTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AGAGCCAGGA GCTTCAGGAG GTCCTGGGGG AGACAGAACG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCAGGGC AGGTGCAGAT CCGGAAGATG 
AAGGCCGTGC ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATT 
GCGGAGGTCT GGTGTGCCAC GAGGGACCTT CCCACTGTAC AGCAGGCGCT GCAGAGCGGC 
TCGAGTGAGG AAGGAGTGAG TGCTGTGGCT CACCGTATCC CTTGCCAGGA CATGCCTCCA 
ACTCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGAT ACAGGGAAGT CAACCCTGCT CCCTACACCA TCATCACATT CCCCTTCCTC 
TTTGCTGTGA TGTTTGGCGA CGTGGGCCAC GGACTGCTCA TGTTTCTCTT CGCCCTGGCT 
ATGGTCCTCA CTGAGAACCA ACCAGCTGTA AAGACCACGC AAAATGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGCTACCT ACCCCTGCTC ATGGGTCTGT TCTCCATCTA CACGGGCTTC 
ATCTACAACG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGCTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACCC CATGCTTACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTC GGACCTTATC CCTTCGGCAT TGACCCGATC 
TGGAGCCTGG CCACCAACCA CCTGAGCTTC CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACACACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCATGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCGTGCCTG AGCTCATCTT CCTGTTGGGC 
CTCTTTGGCT ACCTTGTTTT CCTCATTGTC TACAAGTGGC TCTACGTCTC AGCTGCCAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCCCAAAAC 
CCCACCAATC GGCTGCTCTT CCACGGGCAG GTAGTGGTAC AGTATGCGCT GGTGGTCCTG 
GCTTTGGCCA CAGTTCCTAT CCTGCTGCTG GGCACACCCT TGTACCTTCT GCGCCAGCAC 
CACCGAAGAA ACACTCAGAG AAGGCCTACA GCAGGCAGAC AGGATGAGGA CAGTGACAAG 
CTTCTGGCCT CCCCGGATGC TTCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCT CAGGAGATGA AGAAGCTGAG TTTGTCCCTT CGGAGATCTT CATGCACCAA 
GCCATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC TCATGCTCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TAGGCATGGG CCGAGAGATT GGTGTGGCAT CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCTTGCTAG TGATGGAGGG GCTCTCAGCC 
TTCCTACATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGGACC 
GGCTACAAGC TCAGCCCCTT CGCCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcirg1 ( XM_006230727.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230789.1 Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23724
Clone ID Related Accession (Same CDS sequence)	XM_006230727.2 , XM_008760097.2
Accession Version	XM_006230727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006230727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGCA 
TCTGCCTACA ACTGTGTGAG CCAGCTGGGT GAGCTGGGCC TCGTGGAGTT TAGAGACCTC 
AACGAATCGG TGAGTGCCTT CCAGAGACGC TTCGTGGTAG AGGTCCGGCG CTGCGAGGAG 
CTGGAGAAGA CCTTCACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGCCT GACACTGACC 
CCACCCGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGACCGCT TGGCCCAGGA GCTTCGGGAT GTTCGAGGCA ACCAACAGGC ACTTCGGGCC 
CAGCTGCACC AGCTACAGCT CCACTCAGCC GTGCTGGGCC AGAGCCACGC CCCTCCGATG 
GCAGCTGTCC ATACGGAGGG ACCCACCACT GAGAGAACAC CCCTGCTTTC AGCCACCCCA 
GGGCCACATG CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC TTGCAAGGCT 
GCAGCCCTGG AGCGCCTGCT TTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTAAG 
GAGACTGAGG GGCAGCTGGA GGACCCGGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGGTTC GGAAGATCAC AGACTGCTTT 
CACTGTCATG TCTTCCCGTA CCTGGAGCAG GAGGAAGCCC GGCTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AGAGCCAGGA GCTTCAGGAG GTCCTGGGGG AGACAGAACG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCAGGGC AGGTGCAGAT CCGGAAGATG 
AAGGCCGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATT 
GCGGAGGTCT GGTGTGCCAC GAGGGACCTT CCCACTGTAC AGCAGGCGCT GCAGAGCGGC 
TCGAGTGAGG AAGGAGTGAG TGCTGTGGCT CACCGTATCC CTTGCCAGGA CATGCCTCCA 
ACTCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGAT ACAGGGAAGT CAACCCTGCT CCCTACACCA TCATCACATT CCCCTTCCTC 
TTTGCTGTGA TGTTTGGCGA TGTGGGCCAC GGACTGCTCA TGTTTCTCTT CGCCCTGGCT 
ATGGTCCTCA CTGAGAACCA ACCAGCTGTA AAGACCACGC AAAATGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGCTACCT ACTCCTGCTC ATGGGTCTGT TCTCCATCTA CACGGGCTTC 
ATCTACAACG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGCTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACCC CATGCTTACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTC GGACCTTATC CCTTCGGCAT TGACCCGATC 
TGGAGCCTGG CCACCAACCA CCTGAGCTTC CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACACACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCATGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCGTGCCTG AGCTCATCTT CCTGTTGGGC 
CTCTTTGGCT ACCTTGTTTT CCTCATTGTC TACAAGTGGC TCTACGTCTC AGCTGCCAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCCCAAAAC 
CCCACCAATC GGCTGCTCTT CCACGGGCAG GTAGTGGTAC AGTATGCGCT GGTGGTCCTG 
GCTTTGGCCA CAGTTCCTAT CCTGCTGCTG GGCACACCCT TGTACCTTCT GCGCCAGCAC 
CACCGAAGAA ACACTCAGAG AAGGCCTACA GCAGGCAGAC AGGATGAGGA CAGTGACAAG 
CTTCTGGCCT CCCCGGATGC TTCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCT CAGGAGATGA AGAAGCTGAG TTTGTCCCTT CGGAGATCTT CATGCACCAA 
GCCATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC TCATGCTCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TAGGCATGGG CCGAGAGATT GGTGTGGCAT CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCTTGCTAG TGATGGAGGG GCTCTCAGCC 
TTCCTACATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGGACC 
GGCTACAAGC TCAGCCCCTT CGCCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230789.1
CDS	105..2609
Translation	MGSMFRSEEVALVQLLLPTASAYNCVSQLGELGLVEFRDLNESVSAFQRRFVVEVRRCEELEKTFTFLREEVQRAGLTLTPPEGTLPAPPPRDLLRIQEETDRLAQELRDVRGNQQALRAQLHQLQLHSAVLGQSHAPPMAAVHTEGPTTERTPLLSATPGPHADLKVNFVAGAVEPCKAAALERLLWRACRGFLIASFKETEGQLEDPVTGEPATWMTFVISYWGEQIGQKVRKITDCFHCHVFPYLEQEEARLRTLQQLQQQSQELQEVLGETERFLSQVLGRVQQLLPPGQVQIRKMKAVYLTLNQCSVNTTHKCLIAEVWCATRDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENQPAVKTTQNEIWQTFFGGRYLLLLMGLFSIYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHPMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETVPELIFLLGLFGYLVFLIVYKWLYVSAASASSAPSILIHFINMFLFSQNPTNRLLFHGQVVVQYALVVLALATVPILLLGTPLYLLRQHHRRNTQRRPTAGRQDEDSDKLLASPDASTLENSWSPDEEKAGSSGDEEAEFVPSEIFMHQAIHTIEFCLGCISNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGMGREIGVASVVLVPVFAAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFAFTVDSD

Target ORF information:

RefSeq Version	XM_006230727.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230727.2

ORF Insert Sequence:

ATGGGCTCCA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGCA 
TCTGCCTACA ACTGTGTGAG CCAGCTGGGT GAGCTGGGCC TCGTGGAGTT TAGAGACCTC 
AACGAATCGG TGAGTGCCTT CCAGAGACGC TTCGTGGTAG AGGTCCGGCG CTGCGAGGAG 
CTGGAGAAGA CCTTCACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGCCT GACACTGACC 
CCACCCGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGACCGCT TGGCCCAGGA GCTTCGGGAT GTTCGAGGCA ACCAACAGGC ACTTCGGGCC 
CAGCTGCACC AGCTACAGCT CCACTCAGCC GTGCTGGGCC AGAGCCACGC CCCTCCGATG 
GCAGCTGTCC ATACGGAGGG ACCCACCACT GAGAGAACAC CCCTGCTTTC AGCCACCCCA 
GGGCCACATG CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC TTGCAAGGCT 
GCAGCCCTGG AGCGCCTGCT TTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTAAG 
GAGACTGAGG GGCAGCTGGA GGACCCGGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGGTTC GGAAGATCAC AGACTGCTTT 
CACTGTCATG TCTTCCCGTA CCTGGAGCAG GAGGAAGCCC GGCTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AGAGCCAGGA GCTTCAGGAG GTCCTGGGGG AGACAGAACG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCAGGGC AGGTGCAGAT CCGGAAGATG 
AAGGCCGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATT 
GCGGAGGTCT GGTGTGCCAC GAGGGACCTT CCCACTGTAC AGCAGGCGCT GCAGAGCGGC 
TCGAGTGAGG AAGGAGTGAG TGCTGTGGCT CACCGTATCC CTTGCCAGGA CATGCCTCCA 
ACTCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGAT ACAGGGAAGT CAACCCTGCT CCCTACACCA TCATCACATT CCCCTTCCTC 
TTTGCTGTGA TGTTTGGCGA TGTGGGCCAC GGACTGCTCA TGTTTCTCTT CGCCCTGGCT 
ATGGTCCTCA CTGAGAACCA ACCAGCTGTA AAGACCACGC AAAATGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGCTACCT ACTCCTGCTC ATGGGTCTGT TCTCCATCTA CACGGGCTTC 
ATCTACAACG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGCTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACCC CATGCTTACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTC GGACCTTATC CCTTCGGCAT TGACCCGATC 
TGGAGCCTGG CCACCAACCA CCTGAGCTTC CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACACACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCATGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCGTGCCTG AGCTCATCTT CCTGTTGGGC 
CTCTTTGGCT ACCTTGTTTT CCTCATTGTC TACAAGTGGC TCTACGTCTC AGCTGCCAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCCCAAAAC 
CCCACCAATC GGCTGCTCTT CCACGGGCAG GTAGTGGTAC AGTATGCGCT GGTGGTCCTG 
GCTTTGGCCA CAGTTCCTAT CCTGCTGCTG GGCACACCCT TGTACCTTCT GCGCCAGCAC 
CACCGAAGAA ACACTCAGAG AAGGCCTACA GCAGGCAGAC AGGATGAGGA CAGTGACAAG 
CTTCTGGCCT CCCCGGATGC TTCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCT CAGGAGATGA AGAAGCTGAG TTTGTCCCTT CGGAGATCTT CATGCACCAA 
GCCATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC TCATGCTCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TAGGCATGGG CCGAGAGATT GGTGTGGCAT CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCTTGCTAG TGATGGAGGG GCTCTCAGCC 
TTCCTACATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGGACC 
GGCTACAAGC TCAGCCCCTT CGCCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcirg1 ( XM_008760097.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758319.1 Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X2, mRNA.

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CloneID	ORa23724
Clone ID Related Accession (Same CDS sequence)	XM_006230727.2 , XM_008760097.2
Accession Version	XM_008760097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGCA 
TCTGCCTACA ACTGTGTGAG CCAGCTGGGT GAGCTGGGCC TCGTGGAGTT TAGAGACCTC 
AACGAATCGG TGAGTGCCTT CCAGAGACGC TTCGTGGTAG AGGTCCGGCG CTGCGAGGAG 
CTGGAGAAGA CCTTCACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGCCT GACACTGACC 
CCACCCGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGACCGCT TGGCCCAGGA GCTTCGGGAT GTTCGAGGCA ACCAACAGGC ACTTCGGGCC 
CAGCTGCACC AGCTACAGCT CCACTCAGCC GTGCTGGGCC AGAGCCACGC CCCTCCGATG 
GCAGCTGTCC ATACGGAGGG ACCCACCACT GAGAGAACAC CCCTGCTTTC AGCCACCCCA 
GGGCCACATG CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC TTGCAAGGCT 
GCAGCCCTGG AGCGCCTGCT TTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTAAG 
GAGACTGAGG GGCAGCTGGA GGACCCGGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGGTTC GGAAGATCAC AGACTGCTTT 
CACTGTCATG TCTTCCCGTA CCTGGAGCAG GAGGAAGCCC GGCTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AGAGCCAGGA GCTTCAGGAG GTCCTGGGGG AGACAGAACG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCAGGGC AGGTGCAGAT CCGGAAGATG 
AAGGCCGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATT 
GCGGAGGTCT GGTGTGCCAC GAGGGACCTT CCCACTGTAC AGCAGGCGCT GCAGAGCGGC 
TCGAGTGAGG AAGGAGTGAG TGCTGTGGCT CACCGTATCC CTTGCCAGGA CATGCCTCCA 
ACTCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGAT ACAGGGAAGT CAACCCTGCT CCCTACACCA TCATCACATT CCCCTTCCTC 
TTTGCTGTGA TGTTTGGCGA TGTGGGCCAC GGACTGCTCA TGTTTCTCTT CGCCCTGGCT 
ATGGTCCTCA CTGAGAACCA ACCAGCTGTA AAGACCACGC AAAATGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGCTACCT ACTCCTGCTC ATGGGTCTGT TCTCCATCTA CACGGGCTTC 
ATCTACAACG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGCTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACCC CATGCTTACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTC GGACCTTATC CCTTCGGCAT TGACCCGATC 
TGGAGCCTGG CCACCAACCA CCTGAGCTTC CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACACACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCATGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCGTGCCTG AGCTCATCTT CCTGTTGGGC 
CTCTTTGGCT ACCTTGTTTT CCTCATTGTC TACAAGTGGC TCTACGTCTC AGCTGCCAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCCCAAAAC 
CCCACCAATC GGCTGCTCTT CCACGGGCAG GTAGTGGTAC AGTATGCGCT GGTGGTCCTG 
GCTTTGGCCA CAGTTCCTAT CCTGCTGCTG GGCACACCCT TGTACCTTCT GCGCCAGCAC 
CACCGAAGAA ACACTCAGAG AAGGCCTACA GCAGGCAGAC AGGATGAGGA CAGTGACAAG 
CTTCTGGCCT CCCCGGATGC TTCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCT CAGGAGATGA AGAAGCTGAG TTTGTCCCTT CGGAGATCTT CATGCACCAA 
GCCATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC TCATGCTCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TAGGCATGGG CCGAGAGATT GGTGTGGCAT CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCTTGCTAG TGATGGAGGG GCTCTCAGCC 
TTCCTACATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGGACC 
GGCTACAAGC TCAGCCCCTT CGCCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758319.1
CDS	174..2678
Translation	MGSMFRSEEVALVQLLLPTASAYNCVSQLGELGLVEFRDLNESVSAFQRRFVVEVRRCEELEKTFTFLREEVQRAGLTLTPPEGTLPAPPPRDLLRIQEETDRLAQELRDVRGNQQALRAQLHQLQLHSAVLGQSHAPPMAAVHTEGPTTERTPLLSATPGPHADLKVNFVAGAVEPCKAAALERLLWRACRGFLIASFKETEGQLEDPVTGEPATWMTFVISYWGEQIGQKVRKITDCFHCHVFPYLEQEEARLRTLQQLQQQSQELQEVLGETERFLSQVLGRVQQLLPPGQVQIRKMKAVYLTLNQCSVNTTHKCLIAEVWCATRDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENQPAVKTTQNEIWQTFFGGRYLLLLMGLFSIYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHPMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETVPELIFLLGLFGYLVFLIVYKWLYVSAASASSAPSILIHFINMFLFSQNPTNRLLFHGQVVVQYALVVLALATVPILLLGTPLYLLRQHHRRNTQRRPTAGRQDEDSDKLLASPDASTLENSWSPDEEKAGSSGDEEAEFVPSEIFMHQAIHTIEFCLGCISNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGMGREIGVASVVLVPVFAAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFAFTVDSD

Target ORF information:

RefSeq Version	XM_008760097.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760097.2

ORF Insert Sequence:

ATGGGCTCCA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGCA 
TCTGCCTACA ACTGTGTGAG CCAGCTGGGT GAGCTGGGCC TCGTGGAGTT TAGAGACCTC 
AACGAATCGG TGAGTGCCTT CCAGAGACGC TTCGTGGTAG AGGTCCGGCG CTGCGAGGAG 
CTGGAGAAGA CCTTCACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGCCT GACACTGACC 
CCACCCGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGACCGCT TGGCCCAGGA GCTTCGGGAT GTTCGAGGCA ACCAACAGGC ACTTCGGGCC 
CAGCTGCACC AGCTACAGCT CCACTCAGCC GTGCTGGGCC AGAGCCACGC CCCTCCGATG 
GCAGCTGTCC ATACGGAGGG ACCCACCACT GAGAGAACAC CCCTGCTTTC AGCCACCCCA 
GGGCCACATG CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC TTGCAAGGCT 
GCAGCCCTGG AGCGCCTGCT TTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTAAG 
GAGACTGAGG GGCAGCTGGA GGACCCGGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGGTTC GGAAGATCAC AGACTGCTTT 
CACTGTCATG TCTTCCCGTA CCTGGAGCAG GAGGAAGCCC GGCTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AGAGCCAGGA GCTTCAGGAG GTCCTGGGGG AGACAGAACG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCAGGGC AGGTGCAGAT CCGGAAGATG 
AAGGCCGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATT 
GCGGAGGTCT GGTGTGCCAC GAGGGACCTT CCCACTGTAC AGCAGGCGCT GCAGAGCGGC 
TCGAGTGAGG AAGGAGTGAG TGCTGTGGCT CACCGTATCC CTTGCCAGGA CATGCCTCCA 
ACTCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGAT ACAGGGAAGT CAACCCTGCT CCCTACACCA TCATCACATT CCCCTTCCTC 
TTTGCTGTGA TGTTTGGCGA TGTGGGCCAC GGACTGCTCA TGTTTCTCTT CGCCCTGGCT 
ATGGTCCTCA CTGAGAACCA ACCAGCTGTA AAGACCACGC AAAATGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGCTACCT ACTCCTGCTC ATGGGTCTGT TCTCCATCTA CACGGGCTTC 
ATCTACAACG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGCTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACCC CATGCTTACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTC GGACCTTATC CCTTCGGCAT TGACCCGATC 
TGGAGCCTGG CCACCAACCA CCTGAGCTTC CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACACACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCATGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCGTGCCTG AGCTCATCTT CCTGTTGGGC 
CTCTTTGGCT ACCTTGTTTT CCTCATTGTC TACAAGTGGC TCTACGTCTC AGCTGCCAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCCCAAAAC 
CCCACCAATC GGCTGCTCTT CCACGGGCAG GTAGTGGTAC AGTATGCGCT GGTGGTCCTG 
GCTTTGGCCA CAGTTCCTAT CCTGCTGCTG GGCACACCCT TGTACCTTCT GCGCCAGCAC 
CACCGAAGAA ACACTCAGAG AAGGCCTACA GCAGGCAGAC AGGATGAGGA CAGTGACAAG 
CTTCTGGCCT CCCCGGATGC TTCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCT CAGGAGATGA AGAAGCTGAG TTTGTCCCTT CGGAGATCTT CATGCACCAA 
GCCATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC TCATGCTCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TAGGCATGGG CCGAGAGATT GGTGTGGCAT CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCTTGCTAG TGATGGAGGG GCTCTCAGCC 
TTCCTACATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGGACC 
GGCTACAAGC TCAGCCCCTT CGCCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcirg1 ( XM_006230728.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230790.1 Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa23725
Clone ID Related Accession (Same CDS sequence)	XM_006230728.2
Accession Version	XM_006230728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase 116 kDa subunit a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006230728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG TCCATACGGA GGGACCCACC ACTGAGAGAA CACCCCTGCT TTCAGCCACC 
CCAGGGCCAC ATGCAGACCT GAAGGTCAAT TTTGTGGCAG GTGCTGTGGA GCCTTGCAAG 
GCTGCAGCCC TGGAGCGCCT GCTTTGGAGA GCTTGCCGCG GCTTCCTCAT TGCCAGCTTT 
AAGGAGACTG AGGGGCAGCT GGAGGACCCG GTGACGGGTG AGCCTGCAAC TTGGATGACC 
TTTGTCATCT CCTACTGGGG TGAGCAGATC GGACAGAAGG TTCGGAAGAT CACAGACTGC 
TTTCACTGTC ATGTCTTCCC GTACCTGGAG CAGGAGGAAG CCCGGCTCAG GACCTTGCAG 
CAGCTGCAGC AGCAGAGCCA GGAGCTTCAG GAGGTCCTGG GGGAGACAGA ACGGTTCCTG 
AGCCAGGTGT TGGGCCGGGT GCAGCAGCTG CTGCCCCCAG GGCAGGTGCA GATCCGGAAG 
ATGAAGGCCG TGTACCTGAC CCTCAACCAG TGCAGTGTGA ACACCACACA CAAGTGCCTC 
ATTGCGGAGG TCTGGTGTGC CACGAGGGAC CTTCCCACTG TACAGCAGGC GCTGCAGAGC 
GGCTCGAGTG AGGAAGGAGT GAGTGCTGTG GCTCACCGTA TCCCTTGCCA GGACATGCCT 
CCAACTCTCA TCAGGACCAA CCGCTTCACC TCCAGCTTCC AGGGCATTGT GGATGCCTAT 
GGTGTGGGCC GATACAGGGA AGTCAACCCT GCTCCCTACA CCATCATCAC ATTCCCCTTC 
CTCTTTGCTG TGATGTTTGG CGATGTGGGC CACGGACTGC TCATGTTTCT CTTCGCCCTG 
GCTATGGTCC TCACTGAGAA CCAACCAGCT GTAAAGACCA CGCAAAATGA GATCTGGCAG 
ACCTTCTTTG GGGGTCGCTA CCTACTCCTG CTCATGGGTC TGTTCTCCAT CTACACGGGC 
TTCATCTACA ACGAGTGCTT CAGCCGAGCC ACCACTATTT TCCCCTCAGG CTGGAGTGTG 
GCTGCCATGG CCAACCAGTC AGGCTGGAGT GACGAGTATC TGTCCCAGCA CCCCATGCTT 
ACCCTGAACC CTAACATCAC TGGTGTCTTC CTCGGACCTT ATCCCTTCGG CATTGACCCG 
ATCTGGAGCC TGGCCACCAA CCACCTGAGC TTCCTCAACT CCTTCAAGAT GAAGATGTCT 
GTCATCCTTG GGGTCACACA CATGGCCTTT GGGGTGTTCC TCAGCATCTT CAACCATGTG 
CACTTTGGCC AGGCCCACCG GCTACTGCTG GAGACCGTGC CTGAGCTCAT CTTCCTGTTG 
GGCCTCTTTG GCTACCTTGT TTTCCTCATT GTCTACAAGT GGCTCTACGT CTCAGCTGCC 
AGCGCCTCCT CGGCCCCCAG TATCCTCATT CACTTCATCA ATATGTTCCT CTTCTCCCAA 
AACCCCACCA ATCGGCTGCT CTTCCACGGG CAGGTAGTGG TACAGTATGC GCTGGTGGTC 
CTGGCTTTGG CCACAGTTCC TATCCTGCTG CTGGGCACAC CCTTGTACCT TCTGCGCCAG 
CACCACCGAA GAAACACTCA GAGAAGGCCT ACAGCAGGCA GACAGGATGA GGACAGTGAC 
AAGCTTCTGG CCTCCCCGGA TGCTTCCACC TTGGAGAACA GCTGGAGCCC TGATGAGGAG 
AAGGCTGGGA GCTCAGGAGA TGAAGAAGCT GAGTTTGTCC CTTCGGAGAT CTTCATGCAC 
CAAGCCATCC ACACCATTGA GTTCTGCCTG GGCTGCATCT CCAACACAGC CTCCTACTTG 
CGTCTCTGGG CCCTGAGCCT GGCTCATGCT CAGCTGTCTG AGGTCCTGTG GGCCATGGTG 
ATGCGCATAG GCCTAGGCAT GGGCCGAGAG ATTGGTGTGG CATCTGTGGT GCTGGTCCCT 
GTGTTTGCTG CCTTTGCTGT GTTGACTGTG GCCATCTTGC TAGTGATGGA GGGGCTCTCA 
GCCTTCCTAC ATGCCCTGCG GTTGCACTGG GTGGAGTTCC AGAACAAGTT CTATTCAGGG 
ACCGGCTACA AGCTCAGCCC CTTCGCCTTC ACTGTGGACA GTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230790.1
CDS	493..2580
Translation	MAAVHTEGPTTERTPLLSATPGPHADLKVNFVAGAVEPCKAAALERLLWRACRGFLIASFKETEGQLEDPVTGEPATWMTFVISYWGEQIGQKVRKITDCFHCHVFPYLEQEEARLRTLQQLQQQSQELQEVLGETERFLSQVLGRVQQLLPPGQVQIRKMKAVYLTLNQCSVNTTHKCLIAEVWCATRDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENQPAVKTTQNEIWQTFFGGRYLLLLMGLFSIYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHPMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETVPELIFLLGLFGYLVFLIVYKWLYVSAASASSAPSILIHFINMFLFSQNPTNRLLFHGQVVVQYALVVLALATVPILLLGTPLYLLRQHHRRNTQRRPTAGRQDEDSDKLLASPDASTLENSWSPDEEKAGSSGDEEAEFVPSEIFMHQAIHTIEFCLGCISNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGMGREIGVASVVLVPVFAAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFAFTVDSD

Target ORF information:

RefSeq Version	XM_006230728.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 (Tcirg1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230728.2

ORF Insert Sequence:

ATGGCAGCTG TCCATACGGA GGGACCCACC ACTGAGAGAA CACCCCTGCT TTCAGCCACC 
CCAGGGCCAC ATGCAGACCT GAAGGTCAAT TTTGTGGCAG GTGCTGTGGA GCCTTGCAAG 
GCTGCAGCCC TGGAGCGCCT GCTTTGGAGA GCTTGCCGCG GCTTCCTCAT TGCCAGCTTT 
AAGGAGACTG AGGGGCAGCT GGAGGACCCG GTGACGGGTG AGCCTGCAAC TTGGATGACC 
TTTGTCATCT CCTACTGGGG TGAGCAGATC GGACAGAAGG TTCGGAAGAT CACAGACTGC 
TTTCACTGTC ATGTCTTCCC GTACCTGGAG CAGGAGGAAG CCCGGCTCAG GACCTTGCAG 
CAGCTGCAGC AGCAGAGCCA GGAGCTTCAG GAGGTCCTGG GGGAGACAGA ACGGTTCCTG 
AGCCAGGTGT TGGGCCGGGT GCAGCAGCTG CTGCCCCCAG GGCAGGTGCA GATCCGGAAG 
ATGAAGGCCG TGTACCTGAC CCTCAACCAG TGCAGTGTGA ACACCACACA CAAGTGCCTC 
ATTGCGGAGG TCTGGTGTGC CACGAGGGAC CTTCCCACTG TACAGCAGGC GCTGCAGAGC 
GGCTCGAGTG AGGAAGGAGT GAGTGCTGTG GCTCACCGTA TCCCTTGCCA GGACATGCCT 
CCAACTCTCA TCAGGACCAA CCGCTTCACC TCCAGCTTCC AGGGCATTGT GGATGCCTAT 
GGTGTGGGCC GATACAGGGA AGTCAACCCT GCTCCCTACA CCATCATCAC ATTCCCCTTC 
CTCTTTGCTG TGATGTTTGG CGATGTGGGC CACGGACTGC TCATGTTTCT CTTCGCCCTG 
GCTATGGTCC TCACTGAGAA CCAACCAGCT GTAAAGACCA CGCAAAATGA GATCTGGCAG 
ACCTTCTTTG GGGGTCGCTA CCTACTCCTG CTCATGGGTC TGTTCTCCAT CTACACGGGC 
TTCATCTACA ACGAGTGCTT CAGCCGAGCC ACCACTATTT TCCCCTCAGG CTGGAGTGTG 
GCTGCCATGG CCAACCAGTC AGGCTGGAGT GACGAGTATC TGTCCCAGCA CCCCATGCTT 
ACCCTGAACC CTAACATCAC TGGTGTCTTC CTCGGACCTT ATCCCTTCGG CATTGACCCG 
ATCTGGAGCC TGGCCACCAA CCACCTGAGC TTCCTCAACT CCTTCAAGAT GAAGATGTCT 
GTCATCCTTG GGGTCACACA CATGGCCTTT GGGGTGTTCC TCAGCATCTT CAACCATGTG 
CACTTTGGCC AGGCCCACCG GCTACTGCTG GAGACCGTGC CTGAGCTCAT CTTCCTGTTG 
GGCCTCTTTG GCTACCTTGT TTTCCTCATT GTCTACAAGT GGCTCTACGT CTCAGCTGCC 
AGCGCCTCCT CGGCCCCCAG TATCCTCATT CACTTCATCA ATATGTTCCT CTTCTCCCAA 
AACCCCACCA ATCGGCTGCT CTTCCACGGG CAGGTAGTGG TACAGTATGC GCTGGTGGTC 
CTGGCTTTGG CCACAGTTCC TATCCTGCTG CTGGGCACAC CCTTGTACCT TCTGCGCCAG 
CACCACCGAA GAAACACTCA GAGAAGGCCT ACAGCAGGCA GACAGGATGA GGACAGTGAC 
AAGCTTCTGG CCTCCCCGGA TGCTTCCACC TTGGAGAACA GCTGGAGCCC TGATGAGGAG 
AAGGCTGGGA GCTCAGGAGA TGAAGAAGCT GAGTTTGTCC CTTCGGAGAT CTTCATGCAC 
CAAGCCATCC ACACCATTGA GTTCTGCCTG GGCTGCATCT CCAACACAGC CTCCTACTTG 
CGTCTCTGGG CCCTGAGCCT GGCTCATGCT CAGCTGTCTG AGGTCCTGTG GGCCATGGTG 
ATGCGCATAG GCCTAGGCAT GGGCCGAGAG ATTGGTGTGG CATCTGTGGT GCTGGTCCCT 
GTGTTTGCTG CCTTTGCTGT GTTGACTGTG GCCATCTTGC TAGTGATGGA GGGGCTCTCA 
GCCTTCCTAC ATGCCCTGCG GTTGCACTGG GTGGAGTTCC AGAACAAGTT CTATTCAGGG 
ACCGGCTACA AGCTCAGCCC CTTCGCCTTC ACTGTGGACA GTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autophagy plays a critical role in the degradation of active RHOA, the control of cell cytokinesis, and genomic stability.
Cancer research73(14)4311-22(2013 Jul)
Belaid A,Cerezo M,Chargui A,Corcelle-Termeau E,Pedeutour F,Giuliano S,Ilie M,Rubera I,Tauc M,Barale S,Bertolotto C,Brest P,Vouret-Craviari V,Klionsky DJ,Carle GF,Hofman P,Mograbi B

RNA interference-mediated silencing of Atp6i prevents both periapical bone erosion and inflammation in the mouse model of endodontic disease.
Infection and immunity81(4)1021-30(2013 Apr)
Ma J,Chen W,Zhang L,Tucker B,Zhu G,Sasaki H,Hao L,Wang L,Ci H,Jiang H,Stashenko P,Li YP

Osteopetrosis, osteopetrorickets and hypophosphatemic rickets differentially affect dentin and enamel mineralization.
Bone53(1)25-33(2013 Mar)
Koehne T,Marshall RP,Jeschke A,Kahl-Nieke B,Schinke T,Amling M

Resveratrol improves cardiomyopathy in dystrophin-deficient mice through SIRT1 protein-mediated modulation of p300 protein.
The Journal of biological chemistry288(8)5963-72(2013 Feb)
Kuno A,Hori YS,Hosoda R,Tanno M,Miura T,Shimamoto K,Horio Y

The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2.
The Journal of biological chemistry288(8)5896-913(2013 Feb)
Hosokawa H,Dip PV,Merkulova M,Bakulina A,Zhuang Z,Khatri A,Jian X,Keating SM,Bueler SA,Rubinstein JL,Randazzo PA,Ausiello DA,Gr?ber G,Marshansky V

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Tcirg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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