Fshb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fshb
Entrez Gene ID	25447
Full Name	follicle stimulating hormone beta subunit
Synonyms	FSH-B,FSH-beta
General protein information	Preferred Names follicle stimulating hormone beta subunit Names follitropin subunit beta follicle - stimulating hormone subunit beta follicle stimulating hormone, beta polypeptide follicle-stimulating hormone subunit beta follitropin beta chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q33
Summary	stimulates development of follicle and spermatogenesis in the reproductive organs [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007597.2	NP_001007598.1	follitropin subunit beta precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FSHB	NP_001065282.1
	FSHB	XP_001087914.1
Danio rerio (zebrafish)	fshb	NP_991187.1
Xenopus tropicalis (tropical clawed frog)	fshb	XP_002937726.1
Canis lupus familiaris (dog)	FSHB	XP_542546.2
Gallus gallus (chicken)	FSHB	NP_989588.1
Bos taurus (cattle)	FSHB	NP_776485.1
Rattus norvegicus (Norway rat)	Fshb	NP_001007598.1
Homo sapiens (human)	FSHB	NP_001018090.1
Mus musculus (house mouse)	Fshb	NP_032071.1

Gene Ontology
Bibliography

Related articles in PubMed

Roles of differential expression of microRNA-21-3p and microRNA-433 in FSH regulation in rat anterior pituitary cells.
Han DX, Sun XL, Xu MQ, Chen CZ, Jiang H, Gao Y, Yuan B, Zhang JB
Oncotarget8(22)36553-36565(2017 May)

Long-chain unsaturated fatty acids reduce the transcriptional activity of the rat follicle-stimulating hormone ?-subunit gene.
Moriyama R, Yamazaki T, Kato T, Kato Y
The Journal of reproduction and development62(2)195-9(2016 Apr)

Anti-M?llerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty.
Garrel G, Racine C, L'H?te D, Denoyelle C, Guigon CJ, di Clemente N, Cohen-Tannoudji J
Scientific reports623790(2016 Mar)

Rapid communication: A microRNA-132/212 pathway mediates GnRH activation of FSH expression.
Lannes J, L'H?te D, Garrel G, Laverri?re JN, Cohen-Tannoudji J, Qu?rat B
Molecular endocrinology (Baltimore, Md.)29(3)364-72(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the upstream region of Fshb is susceptible to unsaturated long-chain fatty acids
Title: Long-chain unsaturated fatty acids reduce the transcriptional activity of the rat follicle-stimulating hormone ?-subunit gene.

The study uncovers a new role for AMH in regulating gonadotrope function and suggests that AMH participates in the postnatal elevation of FSH secretion in females.
Title: Anti-M?llerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty.

GnRH stimulation of Fshb expression is dependent on miR-132/212 and involves a SIRT1-FOXO1 pathway.
Title: Rapid communication: A microRNA-132/212 pathway mediates GnRH activation of FSH expression.

FSH acutely regulated CCND2 expression through both PKA and PI3K signaling pathways during granulosa cell proliferation and also accelerated its ubiquitination-proteasomal degradation.
Title: Regulation of cyclin D2 expression and degradation by follicle-stimulating hormone during rat granulosa cell proliferation in vitro.

Data suggest that FSH (Cga/FSHb) induces clock system in granulosa cells by up-regulation of connexin 43 expression; this suggests that gap junctions are implicated in circadian clock in peripheral oscillators besides central pacemaker.
Title: FSH induces the development of circadian clockwork in rat granulosa cells via a gap junction protein Cx43-dependent pathway.

The following Fshb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fshb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa26161	NM_001007597.2 Latest version!	Rattus norvegicus follicle stimulating hormone subunit beta (Fshb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fshb ( NM_001007597.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007598.1 Rattus norvegicus follicle stimulating hormone subunit beta (Fshb), mRNA.

Price & Availability↑

CloneID	ORa26161
Clone ID Related Accession (Same CDS sequence)	NM_001007597.2
Accession Version	NM_001007597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Rattus norvegicus(Norway rat)
Product	follitropin subunit beta precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113706.6. On or before Apr 13, 2016 this sequence version replaced XM_006234672.2, NM_001007597.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC168724.1, CB704014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN04563757 [ECO:0000350] ##Evidence-Data-END##

ATGATGAAGT CGATCCAGCT TTGCATCCTA CTCTGGTGCT TGAGAGCAGT CTGCTGCCAT 
AGCTGTGAAC TGACCAACAT CACCATCTCA GTAGAGAAGG AAGAGTGCCG TTTCTGCATA 
AGCATCAATA CCACTTGGTG TGAGGGCTAC TGCTACACCA GGGATCTGGT GTATAAGGAC 
CCAGCTAGAC CAAACACCCA GAAAGTATGC ACCTTCAAGG AGCTGGTGTA CGAGACCATA 
AGATTGCCTG GCTGTGCCCG CCACTCAGAC TCCCTCTACA CATATCCAGT AGCCACTGAA 
TGCCACTGTG GCAAGTGTGA TAGTGACAGC ACCGACTGCA CTGTAAGAGG CCTGGGACCC 
AGCTACTGCT CCTTCGGTGA AATGAAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007598.1
CDS	63..455
Translation	MMKSIQLCILLWCLRAVCCHSCELTNITISVEKEECRFCISINTTWCEGYCYTRDLVYKDPARPNTQKVCTFKELVYETIRLPGCARHSDSLYTYPVATECHCGKCDSDSTDCTVRGLGPSYCSFGEMKE

Target ORF information:

RefSeq Version	NM_001007597.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus follicle stimulating hormone subunit beta (Fshb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007597.2

ORF Insert Sequence:

ATGATGAAGT CGATCCAGCT TTGCATCCTA CTCTGGTGCT TGAGAGCAGT CTGCTGCCAT 
AGCTGTGAAC TGACCAACAT CACCATCTCA GTAGAGAAGG AAGAGTGCCG TTTCTGCATA 
AGCATCAATA CCACTTGGTG TGAGGGCTAC TGCTACACCA GGGATCTGGT GTATAAGGAC 
CCAGCTAGAC CAAACACCCA GAAAGTATGC ACCTTCAAGG AGCTGGTGTA CGAGACCATA 
AGATTGCCTG GCTGTGCCCG CCACTCAGAC TCCCTCTACA CATATCCAGT AGCCACTGAA 
TGCCACTGTG GCAAGTGTGA TAGTGACAGC ACCGACTGCA CTGTAAGAGG CCTGGGACCC 
AGCTACTGCT CCTTCGGTGA AATGAAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Roles of differential expression of microRNA-21-3p and microRNA-433 in FSH regulation in rat anterior pituitary cells.
Oncotarget8(22)36553-36565(2017 May)
Han DX,Sun XL,Xu MQ,Chen CZ,Jiang H,Gao Y,Yuan B,Zhang JB

Long-chain unsaturated fatty acids reduce the transcriptional activity of the rat follicle-stimulating hormone ?-subunit gene.
The Journal of reproduction and development62(2)195-9(2016 Apr)
Moriyama R,Yamazaki T,Kato T,Kato Y

Anti-M?llerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty.
Scientific reports623790(2016 Mar)
Garrel G,Racine C,L'H?te D,Denoyelle C,Guigon CJ,di Clemente N,Cohen-Tannoudji J

Rapid communication: A microRNA-132/212 pathway mediates GnRH activation of FSH expression.
Molecular endocrinology (Baltimore, Md.)29(3)364-72(2015 Mar)
Lannes J,L'H?te D,Garrel G,Laverri?re JN,Cohen-Tannoudji J,Qu?rat B