Crybb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crybb2
Entrez Gene ID	25422
Full Name	crystallin, beta B2
General protein information	Preferred Names crystallin, beta B2 Names beta-crystallin B2 R.norvegicus CRYBB2 gene (crystallin, beta B2) beta-B2 crystallin beta-crystallin Bp
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	structural protein of the lens of the eye; transiently expressed during development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012937.1	NP_037069.1	beta-crystallin B2
XM_017598273.1	XP_017453762.1	beta-crystallin B2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CRYBB2	NP_777232.1
Danio rerio (zebrafish)	crybb2	NP_001018138.1
Homo sapiens (human)	CRYBB2	NP_000487.1
Canis lupus familiaris (dog)	CRYBB2	NP_001041578.1
Mus musculus (house mouse)	Crybb2	NP_031799.1
Rattus norvegicus (Norway rat)	Crybb2	NP_037069.1
Gallus gallus (chicken)	CRYBB2	NP_990506.2
Pan troglodytes (chimpanzee)	CRYBB2	XP_003317196.1
	CRYBB2	NP_001116366.1
Xenopus tropicalis (tropical clawed frog)	crybb2	NP_001238879.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein-protein interactions among human lens acidic and basic beta-crystallins.
Liu BF, Liang JJ
FEBS letters581(21)3936-42(2007 Aug)

Elongation of axons during regeneration involves retinal crystallin beta b2 (crybb2).
Liedtke T, Schwamborn JC, Schr?er U, Thanos S
Molecular & cellular proteomics : MCP6(5)895-907(2007 May)

Domain interaction sites of human lens betaB2-crystallin.
Liu BF, Liang JJ
The Journal of biological chemistry281(5)2624-30(2006 Feb)

Energetics of domain-domain interactions and entropy driven association of beta-crystallins.
Sergeev YV, Hejtmancik JF, Wingfield PT
Biochemistry43(2)415-24(2004 Jan)

Lens proteomics: analysis of rat crystallin sequences and two-dimensional electrophoresis map.
Lampi KJ, Shih M, Ueda Y, Shearer TR, David LL
Investigative ophthalmology & visual science43(1)216-24(2002 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data are the first to show that axonal regeneration is related to crybb2 movement.
Title: Elongation of axons during regeneration involves retinal crystallin beta b2 (crybb2).

The following Crybb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crybb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01372	NM_012937.1 Latest version!	Rattus norvegicus crystallin, beta B2 (Crybb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa57784	XM_017598273.1 Latest version!	Rattus norvegicus crystallin, beta B2 (Crybb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crybb2 ( NM_012937.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037069.1 Rattus norvegicus crystallin, beta B2 (Crybb2), mRNA.

Price & Availability↑

CloneID	ORa01372
Clone ID Related Accession (Same CDS sequence)	NM_012937.1
Accession Version	NM_012937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	beta-crystallin B2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X16072.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16072.1, CA997768.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC TAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCATGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGGATGGA GAAGGCAGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGAGAG CAGTTTGTGT TTGAGAAGGG CGAGTACCCA 
CGCTGGGACT CCTGGACCAG CAGCCGGAGG ACGGACTCCC TCAGTTCTCT GAGACCCATC 
AAAGTGGACA GCCAGGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACGGGCAAG 
AAGATGGAGA TTGTCGATGA TGACGTACCC AGCTTTCACG CTCACGGATA CCAAGAAAAA 
GTGTCTTCCG TGCGCGTGCA GAGCGGCACG TGGGTTGGGT ATCAGTACCC TGGCTACCGT 
GGGCTGCAGT ATCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAATCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037069.1
CDS	27..644
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELSGPCPNLKETGMEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIVDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDNSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	NM_012937.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus crystallin, beta B2 (Crybb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012937.1

ORF Insert Sequence:

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC TAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCATGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGGATGGA GAAGGCAGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGAGAG CAGTTTGTGT TTGAGAAGGG CGAGTACCCA 
CGCTGGGACT CCTGGACCAG CAGCCGGAGG ACGGACTCCC TCAGTTCTCT GAGACCCATC 
AAAGTGGACA GCCAGGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACGGGCAAG 
AAGATGGAGA TTGTCGATGA TGACGTACCC AGCTTTCACG CTCACGGATA CCAAGAAAAA 
GTGTCTTCCG TGCGCGTGCA GAGCGGCACG TGGGTTGGGT ATCAGTACCC TGGCTACCGT 
GGGCTGCAGT ATCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAATCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crybb2 ( XM_017598273.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453762.1 Rattus norvegicus crystallin, beta B2 (Crybb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57784
Clone ID Related Accession (Same CDS sequence)	XM_017598273.1
Accession Version	XM_017598273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-crystallin B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC TAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCATGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGGATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGAGAG CAGTTTGTGT TTGAGAAGGG CGAGTACCCA 
CGCTGGGACT CCTGGACCAG CAGCCGGAGG ACGGACTCCC TCAGTTCTCT GAGACCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACGGGCAAG 
AAGATGGAGA TTGTCGATGA TGACGTACCC AGCTTTCACG CTCACGGATA CCAAGAAAAA 
GTGTCTTCCG TGCGCGTGCA GAGCGGCACG TGGGTTGGGT ATCAGTACCC TGGCTACCGT 
GGGCTGCAGT ATCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAATCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453762.1
CDS	97..714
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELSGPCPNLKETGMEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIVDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDNSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	XM_017598273.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus crystallin, beta B2 (Crybb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598273.1

ORF Insert Sequence:

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC TAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCATGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGGATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGAGAG CAGTTTGTGT TTGAGAAGGG CGAGTACCCA 
CGCTGGGACT CCTGGACCAG CAGCCGGAGG ACGGACTCCC TCAGTTCTCT GAGACCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACGGGCAAG 
AAGATGGAGA TTGTCGATGA TGACGTACCC AGCTTTCACG CTCACGGATA CCAAGAAAAA 
GTGTCTTCCG TGCGCGTGCA GAGCGGCACG TGGGTTGGGT ATCAGTACCC TGGCTACCGT 
GGGCTGCAGT ATCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAATCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein-protein interactions among human lens acidic and basic beta-crystallins.
FEBS letters581(21)3936-42(2007 Aug)
Liu BF,Liang JJ

Elongation of axons during regeneration involves retinal crystallin beta b2 (crybb2).
Molecular & cellular proteomics : MCP6(5)895-907(2007 May)
Liedtke T,Schwamborn JC,Schr?er U,Thanos S

Domain interaction sites of human lens betaB2-crystallin.
The Journal of biological chemistry281(5)2624-30(2006 Feb)
Liu BF,Liang JJ

Energetics of domain-domain interactions and entropy driven association of beta-crystallins.
Biochemistry43(2)415-24(2004 Jan)
Sergeev YV,Hejtmancik JF,Wingfield PT

Lens proteomics: analysis of rat crystallin sequences and two-dimensional electrophoresis map.
Investigative ophthalmology & visual science43(1)216-24(2002 Jan)
Lampi KJ,Shih M,Ueda Y,Shearer TR,David LL