Cst3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cst3
Entrez Gene ID	25307
Full Name	cystatin C
Synonyms	CYSC
General protein information	Preferred Names cystatin C Names cystatin-C Cystatin C (cysteine proteinase inhibitor) cystatin-3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	extracellular cysteine protease inhibitor that may be involved in amyloidosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012837.1	NP_036969.1	cystatin-C precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC607874	XP_003639869.1
Pan troglodytes (chimpanzee)	CST3	XP_001146702.2
	CST3	NP_001028096.1
Gallus gallus (chicken)	CST3	NP_990831.2
Homo sapiens (human)	CST3	NP_000090.1
Xenopus tropicalis (tropical clawed frog)	LOC100485774	XP_002934915.1
Bos taurus (cattle)	CST3	NP_776454.1
Mus musculus (house mouse)	Cst3	NP_034106.2
Rattus norvegicus (Norway rat)	Cst3	NP_036969.1

Gene Ontology
Bibliography

Related articles in PubMed

RIPK1 mediates a disease-associated microglial response in Alzheimer's disease.
Ofengeim D, Mazzitelli S, Ito Y, DeWitt JP, Mifflin L, Zou C, Das S, Adiconis X, Chen H, Zhu H, Kelliher MA, Levin JZ, Yuan J
Proceedings of the National Academy of Sciences of the United States of America114(41)E8788-E8797(2017 Oct)

Protective effect of tanshinone IIA against cardiac hypertrophy in spontaneously hypertensive rats through inhibiting the Cys-C/Wnt signaling pathway.
Feng J, Chen HW, Pi LJ, Wang J, Zhan DQ
Oncotarget8(6)10161-10170(2017 Feb)

Amyloid properties of the leader peptide of variant B cystatin C: implications for Alzheimer and macular degeneration.
Sant'Anna R, Navarro S, Ventura S, Paraoan L, Foguel D
FEBS letters590(5)644-54(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that Cd increases the urinary excretion of cystatin C, and they suggest that this effect may involve disruption of megalin-mediated uptake of cystatin C by epithelial cells of the proximal tubule.
Title: Evaluation of cystatin C as an early biomarker of cadmium nephrotoxicity in the rat.

High cystatin C is associated with proteinuria.
Title: Albuminuria increases cystatin C excretion: implications for urinary biomarkers.

Triiodothyronine-induced increase in the production of cystatin C may be related to an increased cell metabolism and proteolysis control demand.
Title: Triiodothyronine stimulates cystatin C production in bone cells.

Oxidants induce elevated cystatin C production from cardiac myocytes. Cystatin C plays a role in cardiac extracellular matrix remodelling.
Title: Cystatin C increases in cardiac injury: a role in extracellular matrix protein modulation.

CysC plays a protective role under conditions of neuronal challenge by inducing autophagy via mTOR inhibition
Title: Induction of autophagy by cystatin C: a mechanism that protects murine primary cortical neurons and neuronal cell lines.

The following Cst3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cst3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01083	NM_012837.1 Latest version!	Rattus norvegicus cystatin C (Cst3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cst3 ( NM_012837.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036969.1 Rattus norvegicus cystatin C (Cst3), mRNA.

Price & Availability↑

CloneID	ORa01083
Clone ID Related Accession (Same CDS sequence)	NM_012837.1
Accession Version	NM_012837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cystatin-C precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB692474.1, CB608544.1 and CB314369.1. On Oct 18, 2004 this sequence version replaced XM_342538.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ218053.1, FQ212057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAGCC CGCTGCGCTC CTTGATGCTA CTGCTGGCCG TCCTGGCCGT GGCCTGGGCC 
GGAACCTCCA GGCCACCCCC GCGATTGTTG GGAGCTCCGC AGGAGGCAGA TGCCAGCGAG 
GAGGGCGTGC AGCGAGCGTT GGACTTCGCC GTAAGCGAGT ACAACAAGGG CAGCAACGAT 
GCGTACCACA GCCGCGCCAT ACAGGTGGTG AGAGCTCGTA AGCAGCTTGT GGCTGGAATA 
AACTATTATT TGGATGTGGA GATGGGCCGA ACTACATGTA CCAAGTCCCA GACAAATTTG 
ACTAACTGTC CTTTCCACGA CCAGCCCCAT CTGATGAGGA AGGCACTCTG CTCCTTCCAG 
ATCTACAGCG TGCCCTGGAA AGGCACACAC ACCCTGACAA AATCCAGCTG CAAAAATGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036969.1
CDS	70..492
Translation	MASPLRSLMLLLAVLAVAWAGTSRPPPRLLGAPQEADASEEGVQRALDFAVSEYNKGSNDAYHSRAIQVVRARKQLVAGINYYLDVEMGRTTCTKSQTNLTNCPFHDQPHLMRKALCSFQIYSVPWKGTHTLTKSSCKNA

Target ORF information:

RefSeq Version	NM_012837.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cystatin C (Cst3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012837.1

ORF Insert Sequence:

ATGGCCAGCC CGCTGCGCTC CTTGATGCTA CTGCTGGCCG TCCTGGCCGT GGCCTGGGCC 
GGAACCTCCA GGCCACCCCC GCGATTGTTG GGAGCTCCGC AGGAGGCAGA TGCCAGCGAG 
GAGGGCGTGC AGCGAGCGTT GGACTTCGCC GTAAGCGAGT ACAACAAGGG CAGCAACGAT 
GCGTACCACA GCCGCGCCAT ACAGGTGGTG AGAGCTCGTA AGCAGCTTGT GGCTGGAATA 
AACTATTATT TGGATGTGGA GATGGGCCGA ACTACATGTA CCAAGTCCCA GACAAATTTG 
ACTAACTGTC CTTTCCACGA CCAGCCCCAT CTGATGAGGA AGGCACTCTG CTCCTTCCAG 
ATCTACAGCG TGCCCTGGAA AGGCACACAC ACCCTGACAA AATCCAGCTG CAAAAATGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RIPK1 mediates a disease-associated microglial response in Alzheimer's disease.
Proceedings of the National Academy of Sciences of the United States of America114(41)E8788-E8797(2017 Oct)
Ofengeim D,Mazzitelli S,Ito Y,DeWitt JP,Mifflin L,Zou C,Das S,Adiconis X,Chen H,Zhu H,Kelliher MA,Levin JZ,Yuan J

Protective effect of tanshinone IIA against cardiac hypertrophy in spontaneously hypertensive rats through inhibiting the Cys-C/Wnt signaling pathway.
Oncotarget8(6)10161-10170(2017 Feb)
Feng J,Chen HW,Pi LJ,Wang J,Zhan DQ

Amyloid properties of the leader peptide of variant B cystatin C: implications for Alzheimer and macular degeneration.
FEBS letters590(5)644-54(2016 Mar)
Sant'Anna R,Navarro S,Ventura S,Paraoan L,Foguel D