Prlhr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prlhr
Entrez Gene ID	246075
Full Name	prolactin releasing hormone receptor
Synonyms	Gpr10,Uhr-1
General protein information	Preferred Names prolactin releasing hormone receptor Names prolactin-releasing peptide receptor G-protein coupled receptor 10 prRP receptor prRPR
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q55
Summary	putative receptor for prolactin-releasing peptide [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_139193.3	NP_631932.3	prolactin-releasing peptide receptor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRLHR	NP_004239.1
Gallus gallus (chicken)	PRLHR	NP_001012295.1
Drosophila melanogaster (fruit fly)	sNPF-R	NP_001262086.1
Rattus norvegicus (Norway rat)	Prlhr	NP_631932.3
Xenopus tropicalis (tropical clawed frog)	prlhr	XP_002940396.1
Pan troglodytes (chimpanzee)	PRLHR	XP_521659.1
Canis lupus familiaris (dog)	PRLHR	XP_544041.2
Mus musculus (house mouse)	Prlhr	NP_963909.2
Bos taurus (cattle)	PRLHR	NP_001025471.1

Gene Ontology
Bibliography

Related articles in PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
Badgandi HB, Hwang SH, Shimada IS, Loriot E, Mukhopadhyay S
The Journal of cell biology216(3)743-760(2017 Mar)

Blockade of PrRP attenuates MPTP-induced toxicity in mice.
Sun B, Mochiduki A, Nakamura K, Yokoyama K, Adachi S, Fujiwara K, Matsumoto H, Inoue K
Peptides30(7)1267-75(2009 Jul)

Mutated G-protein-coupled receptor GPR10 is responsible for the hyperphagia/dyslipidaemia/obesity locus of Dmo1 in the OLETF rat.
Watanabe TK, Suzuki M, Yamasaki Y, Okuno S, Hishigaki H, Ono T, Oga K, Mizoguchi-Miyakita A, Tsuji A, Kanemoto N, Wakitani S, Takagi T, Nakamura Y, Tanigami A
Clinical and experimental pharmacology & physiology32(5-6)355-66(2005 May-Jun)

Characterization of a naturally-occurring polymorphism in the UHR-1 gene encoding the putative rat prolactin-releasing peptide receptor.
Ellacott KL, Donald EL, Clarkson P, Morten J, Masters D, Brennand J, Luckman SM
Peptides26(4)675-81(2005 Apr)

The prolactin-releasing peptide receptor (GPR10) regulates body weight homeostasis in mice.
Gu W, Geddes BJ, Zhang C, Foley KP, Stricker-Krongrad A
Journal of molecular neuroscience : MN22(1-2)93-103(2004)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data report that PrRP and its receptor, GPR10, were found in the mouse substantia nigra pars compacta (SNpc), the main location of dopaminergic (DA) neurons of the nigrostriatal system.
Title: Blockade of PrRP attenuates MPTP-induced toxicity in mice.

the mutated GPR10 receptor is responsible for the hyperphagia leading to obesity and dyslipidemia in the obese diabetic OLETF strain rat.
Title: Mutated G-protein-coupled receptor GPR10 is responsible for the hyperphagia/dyslipidaemia/obesity locus of Dmo1 in the OLETF rat.

naturally occurring polymorphism in the UHR-1 gene that results in an ATG to ATA change at the putative translational initiation site.
Title: Characterization of a naturally-occurring polymorphism in the UHR-1 gene encoding the putative rat prolactin-releasing peptide receptor.

The receptor UHR1/GPR10 gene was expressed consistently in the medullary reticular formation and the adrenal gland but also transiently in several locations.
Title: Expression of neuropeptide FF, prolactin-releasing peptide, and the receptor UHR1/GPR10 genes during embryogenesis in the rat.

The following Prlhr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prlhr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02270	NM_139193.3 Latest version!	Rattus norvegicus prolactin releasing hormone receptor (Prlhr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prlhr ( NM_139193.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_631932.3 Rattus norvegicus prolactin releasing hormone receptor (Prlhr), mRNA.

Price & Availability↑

CloneID	ORa02270
Clone ID Related Accession (Same CDS sequence)	NM_139193.3
Accession Version	NM_139193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	prolactin-releasing peptide receptor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S77867.1. On Apr 28, 2006 this sequence version replaced NM_139193.2. ##Evidence-Data-START## Transcript exon combination :: S77867.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCTCAC TGCCCCCTGG AACCACTGGG GACCCCGATT TGTTTTCTGG GCCGTCGCCA 
GCCGGCTCCA CTCCAGCCAA CCAGAGTGCA GAGGCTTCAG AGAGCAATGT GTCTGCGACG 
GTTCCCAGAG CTGCAGCAGT CACGCCGTTC CAGAGCCTGC AACTAGTGCA CCAGCTGAAG 
GGACTGATCG TGATGCTGTA CAGCATCGTG GTGGTCGTGG GTCTGGTGGG CAACTGCCTT 
CTTGTGCTGG TGATCGCGCG CGTGCGCCGG CTGCACAACG TGACCAACTT CCTCATCGGC 
AACCTGGCCT TGTCCGATGT GCTCATGTGT GCCGCCTGTG TGCCTCTCAC GCTGGCCTAC 
GCCTTTGAAC CTCGTGGCTG GGTGTTCGGT GGAGGCCTGT GCCACCTTGT TTTCTTCCTG 
CAGCCGGTCA CCGTCTACGT ATCGGTGTTC ACACTCACCA CAATCGCTGT GGACCGCTAT 
GTGGTTCTGG TGCACCCGCT ACGTCGGCGC ATTTCACTGA AGCTCAGCGC CTACGCTGTG 
CTGGGCATCT GGGCTCTATC TGCAGTGCTG GCGCTGCCGG CCGCGGTGCA CACCTACCAT 
GTAGAGCTCA AGCCCCACGA CGTGCGCCTC TGCGAGGAGT TCTGGGGTTC GCAGGAGCGC 
CAGAGACAGA TCTATGCCTG GGGGCTGCTG CTGGGCACCT ATTTGCTCCC CCTGCTGGCC 
ATTCTCCTGT CTTACGTCCG GGTGTCGGTG AAGTTGCGGA ACCGCGTGGT GCCTGGCAGC 
GTGACCCAGA GCCAGGCTGA CTGGGACCGA GCGCGTCGCC GTCGCACTTT CTGCCTGCTG 
GTGGTGGTGG TGGTCGTGTT CGCGCTCTGC TGGCTGCCTC TGCACATTTT CAACCTTCTG 
CGGGACCTGG ACCCGCGTGC CATCGACCCC TACGCCTTCG GGCTGGTGCA GCTCCTCTGC 
CACTGGCTTG CCATGAGCTC CGCCTGCTAC AACCCCTTCA TCTATGCGTG GCTGCACGAC 
AGCTTCCGAG AGGAGCTACG CAAGATGCTT CTGTCTTGGC CCCGCAAGAT CGTGCCTCAT 
GGCCAGAATA TGACCGTCAG TGTGGTCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_631932.3
CDS	21..1133
Translation	MTSLPPGTTGDPDLFSGPSPAGSTPANQSAEASESNVSATVPRAAAVTPFQSLQLVHQLKGLIVMLYSIVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCAACVPLTLAYAFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISLKLSAYAVLGIWALSAVLALPAAVHTYHVELKPHDVRLCEEFWGSQERQRQIYAWGLLLGTYLLPLLAILLSYVRVSVKLRNRVVPGSVTQSQADWDRARRRRTFCLLVVVVVVFALCWLPLHIFNLLRDLDPRAIDPYAFGLVQLLCHWLAMSSACYNPFIYAWLHDSFREELRKMLLSWPRKIVPHGQNMTVSVVI

Target ORF information:

RefSeq Version	NM_139193.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prolactin releasing hormone receptor (Prlhr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139193.3

ORF Insert Sequence:

ATGACCTCAC TGCCCCCTGG AACCACTGGG GACCCCGATT TGTTTTCTGG GCCGTCGCCA 
GCCGGCTCCA CTCCAGCCAA CCAGAGTGCA GAGGCTTCAG AGAGCAATGT GTCTGCGACG 
GTTCCCAGAG CTGCAGCAGT CACGCCGTTC CAGAGCCTGC AACTAGTGCA CCAGCTGAAG 
GGACTGATCG TGATGCTGTA CAGCATCGTG GTGGTCGTGG GTCTGGTGGG CAACTGCCTT 
CTTGTGCTGG TGATCGCGCG CGTGCGCCGG CTGCACAACG TGACCAACTT CCTCATCGGC 
AACCTGGCCT TGTCCGATGT GCTCATGTGT GCCGCCTGTG TGCCTCTCAC GCTGGCCTAC 
GCCTTTGAAC CTCGTGGCTG GGTGTTCGGT GGAGGCCTGT GCCACCTTGT TTTCTTCCTG 
CAGCCGGTCA CCGTCTACGT ATCGGTGTTC ACACTCACCA CAATCGCTGT GGACCGCTAT 
GTGGTTCTGG TGCACCCGCT ACGTCGGCGC ATTTCACTGA AGCTCAGCGC CTACGCTGTG 
CTGGGCATCT GGGCTCTATC TGCAGTGCTG GCGCTGCCGG CCGCGGTGCA CACCTACCAT 
GTAGAGCTCA AGCCCCACGA CGTGCGCCTC TGCGAGGAGT TCTGGGGTTC GCAGGAGCGC 
CAGAGACAGA TCTATGCCTG GGGGCTGCTG CTGGGCACCT ATTTGCTCCC CCTGCTGGCC 
ATTCTCCTGT CTTACGTCCG GGTGTCGGTG AAGTTGCGGA ACCGCGTGGT GCCTGGCAGC 
GTGACCCAGA GCCAGGCTGA CTGGGACCGA GCGCGTCGCC GTCGCACTTT CTGCCTGCTG 
GTGGTGGTGG TGGTCGTGTT CGCGCTCTGC TGGCTGCCTC TGCACATTTT CAACCTTCTG 
CGGGACCTGG ACCCGCGTGC CATCGACCCC TACGCCTTCG GGCTGGTGCA GCTCCTCTGC 
CACTGGCTTG CCATGAGCTC CGCCTGCTAC AACCCCTTCA TCTATGCGTG GCTGCACGAC 
AGCTTCCGAG AGGAGCTACG CAAGATGCTT CTGTCTTGGC CCCGCAAGAT CGTGCCTCAT 
GGCCAGAATA TGACCGTCAG TGTGGTCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
The Journal of cell biology216(3)743-760(2017 Mar)
Badgandi HB,Hwang SH,Shimada IS,Loriot E,Mukhopadhyay S

Blockade of PrRP attenuates MPTP-induced toxicity in mice.
Peptides30(7)1267-75(2009 Jul)
Sun B,Mochiduki A,Nakamura K,Yokoyama K,Adachi S,Fujiwara K,Matsumoto H,Inoue K

Mutated G-protein-coupled receptor GPR10 is responsible for the hyperphagia/dyslipidaemia/obesity locus of Dmo1 in the OLETF rat.
Clinical and experimental pharmacology & physiology32(5-6)355-66(2005 May-Jun)
Watanabe TK,Suzuki M,Yamasaki Y,Okuno S,Hishigaki H,Ono T,Oga K,Mizoguchi-Miyakita A,Tsuji A,Kanemoto N,Wakitani S,Takagi T,Nakamura Y,Tanigami A

Characterization of a naturally-occurring polymorphism in the UHR-1 gene encoding the putative rat prolactin-releasing peptide receptor.
Peptides26(4)675-81(2005 Apr)
Ellacott KL,Donald EL,Clarkson P,Morten J,Masters D,Brennand J,Luckman SM

The prolactin-releasing peptide receptor (GPR10) regulates body weight homeostasis in mice.
Journal of molecular neuroscience : MN22(1-2)93-103(2004)
Gu W,Geddes BJ,Zhang C,Foley KP,Stricker-Krongrad A