Pkia cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkia
Entrez Gene ID	114906
Full Name	cAMP-dependent protein kinase inhibitor alpha
General protein information	Preferred Names cAMP-dependent protein kinase inhibitor alpha Names cAMP-dependent protein kinase inhibitor alpha PKI-alpha cAMP-dependent protein kinase inhibitor, muscle/brain isoform protein kinase (cAMP-dependent, catalytic) inhibitor alpha protein kinase inhibitor, alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q23
Summary	inhibits cAMP-dependent protein kinase; may bind to the protein kinase catalytic site [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006232153.3	XP_006232215.1	cAMP-dependent protein kinase inhibitor alpha isoform X2
NM_053772.2	NP_446224.1	cAMP-dependent protein kinase inhibitor alpha
XM_008760857.2	XP_008759079.1	cAMP-dependent protein kinase inhibitor alpha isoform X1
XM_006232155.3	XP_006232217.1	cAMP-dependent protein kinase inhibitor alpha isoform X3
XM_006232156.3	XP_006232218.1	cAMP-dependent protein kinase inhibitor alpha isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	PKIA	NP_862822.1
Canis lupus familiaris (dog)	PKIA	XP_853383.2
Xenopus tropicalis (tropical clawed frog)	pkia	NP_001025580.1
Bos taurus (cattle)	PKIA	NP_001032709.1
Rattus norvegicus (Norway rat)	Pkia	NP_446224.1
Pan troglodytes (chimpanzee)	PKIA	XP_003311822.1
	PKIA	XP_002805436.1
Gallus gallus (chicken)	PKIA	NP_990705.1
Mus musculus (house mouse)	Pkia	NP_032888.1

Gene Ontology
Bibliography

Related articles in PubMed

The testis-specific C?2 subunit of PKA is kinetically indistinguishable from the common C?1 subunit of PKA.
Vetter MM, Zenn HM, M?ndez E, van den Boom H, Herberg FW, Sk?lhegg BS
BMC biochemistry1240(2011 Aug)

Characterization of PKIgamma, a novel isoform of the protein kinase inhibitor of cAMP-dependent protein kinase.
Collins SP, Uhler MD
The Journal of biological chemistry272(29)18169-78(1997 Jul)

The expression and intracellular distribution of the heat-stable protein kinase inhibitor is cell cycle regulated.
Wen W, Taylor SS, Meinkoth JL
The Journal of biological chemistry270(5)2041-6(1995 Feb)

Glutamic acid 203 of the cAMP-dependent protein kinase catalytic subunit participates in the inhibition by two isoforms of the protein kinase inhibitor.
Baude EJ, Dignam SS, Olsen SR, Reimann EM, Uhler MD
The Journal of biological chemistry269(3)2316-23(1994 Jan)

Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase.
Knighton DR, Zheng JH, Ten Eyck LF, Ashford VA, Xuong NH, Taylor SS, Sowadski JM
Science (New York, N.Y.)253(5018)407-14(1991 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pkia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10591	NM_053772.2 Latest version!	Rattus norvegicus cAMP-dependent protein kinase inhibitor alpha (Pkia), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10591	XM_006232155.3 Latest version!	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10591	XM_006232156.3 Latest version!	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa24541	XM_006232153.3 Latest version!	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa43966	XM_008760857.2 Latest version!	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkia ( NM_053772.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446224.1 Rattus norvegicus cAMP-dependent protein kinase inhibitor alpha (Pkia), mRNA.

Price & Availability↑

CloneID	ORa10591
Clone ID Related Accession (Same CDS sequence)	NM_053772.2 , XM_006232155.3 , XM_006232156.3
Accession Version	NM_053772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase inhibitor alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM055544.1 and AABR07009404.1. On Dec 23, 2009 this sequence version replaced NM_053772.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FM055544.1, FN800013.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGACTGATG TGGAAACTAC GTATGCAGAT TTTATTGCCT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCTT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTG 
AAATTAGCAG GTCTTGATAT TAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACAGAACAAA GTGGCGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446224.1
CDS	377..607
Translation	MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	NM_053772.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cAMP-dependent protein kinase inhibitor alpha (Pkia), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053772.2

ORF Insert Sequence:

ATGACTGATG TGGAAACTAC GTATGCAGAT TTTATTGCCT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCTT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTG 
AAATTAGCAG GTCTTGATAT TAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACAGAACAAA GTGGCGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkia ( XM_006232155.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232217.1 Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa10591
Clone ID Related Accession (Same CDS sequence)	NM_053772.2 , XM_006232155.3 , XM_006232156.3
Accession Version	XM_006232155.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase inhibitor alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232155.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGATG TGGAAACTAC GTATGCAGAT TTTATTGCCT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCTT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTG 
AAATTAGCAG GTCTTGATAT TAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACAGAACAAA GTGGCGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232217.1
CDS	228..458
Translation	MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	XM_006232155.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232155.3

ORF Insert Sequence:

ATGACTGATG TGGAAACTAC GTATGCAGAT TTTATTGCCT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCTT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTG 
AAATTAGCAG GTCTTGATAT TAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACAGAACAAA GTGGCGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkia ( XM_006232156.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232218.1 Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa10591
Clone ID Related Accession (Same CDS sequence)	NM_053772.2 , XM_006232155.3 , XM_006232156.3
Accession Version	XM_006232156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase inhibitor alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGATG TGGAAACTAC GTATGCAGAT TTTATTGCCT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCTT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTG 
AAATTAGCAG GTCTTGATAT TAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACAGAACAAA GTGGCGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232218.1
CDS	2268..2498
Translation	MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	XM_006232156.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232156.3

ORF Insert Sequence:

ATGACTGATG TGGAAACTAC GTATGCAGAT TTTATTGCCT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCTT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTG 
AAATTAGCAG GTCTTGATAT TAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACAGAACAAA GTGGCGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkia ( XM_006232153.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232215.1 Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24541
Clone ID Related Accession (Same CDS sequence)	XM_006232153.3
Accession Version	XM_006232153.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase inhibitor alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232153.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAAT GTCACTATAA AAATCATCTT AGCAGCTTCG TAGGCTCAGT TTCTTCGAGG 
ATACCCCGAT GTGGAAAGCT TCAGTATAAG CTAGGAAGCT CCCTGCTATG TGGATATTTG 
TTAGCAATGA CTGATGTGGA AACTACGTAT GCAGATTTTA TTGCCTCAGG AAGAACAGGT 
AGAAGAAATG CAATACATGA TATCTTGGTT TCCTCTGCAA GTGGCAACAG CAATGAATTA 
GCCTTGAAAT TAGCAGGTCT TGATATTAAC AAGACAGAAG GTGAAGATGA TGGACAGAGA 
AGCTCCACAG AACAAAGTGG CGAAGCCCAG GGAGAAGCAG CCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232215.1
CDS	441..797
Translation	MTQCHYKNHLSSFVGSVSSRIPRCGKLQYKLGSSLLCGYLLAMTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	XM_006232153.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232153.3

ORF Insert Sequence:

ATGACTCAAT GTCACTATAA AAATCATCTT AGCAGCTTCG TAGGCTCAGT TTCTTCGAGG 
ATACCCCGAT GTGGAAAGCT TCAGTATAAG CTAGGAAGCT CCCTGCTATG TGGATATTTG 
TTAGCAATGA CTGATGTGGA AACTACGTAT GCAGATTTTA TTGCCTCAGG AAGAACAGGT 
AGAAGAAATG CAATACATGA TATCTTGGTT TCCTCTGCAA GTGGCAACAG CAATGAATTA 
GCCTTGAAAT TAGCAGGTCT TGATATTAAC AAGACAGAAG GTGAAGATGA TGGACAGAGA 
AGCTCCACAG AACAAAGTGG CGAAGCCCAG GGAGAAGCAG CCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkia ( XM_008760857.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759079.1 Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43966
Clone ID Related Accession (Same CDS sequence)	XM_008760857.2
Accession Version	XM_008760857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase inhibitor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAAT GTCACTATAA AAATCATCTT AGCAGCTTCG TAGGCTCAGT TTCTTCGAGG 
ATACCCCGAT GTGGAAAGCT TCAGTATAAG CTAGGAAGCC TTTCTTCTTC CAGATACACA 
GTGTCCTCTA GAATTCCCTA TCACAAAGCA GGCTGGTCCA CTAGCTTTTT GGCATGGAAC 
AAGGAAGACA GCAAACATCT AAGGACAATT AAATATGTTT CCCTGCTATG TGGATATTTG 
TTAGCAATGA CTGATGTGGA AACTACGTAT GCAGATTTTA TTGCCTCAGG AAGAACAGGT 
AGAAGAAATG CAATACATGA TATCTTGGTT TCCTCTGCAA GTGGCAACAG CAATGAATTA 
GCCTTGAAAT TAGCAGGTCT TGATATTAAC AAGACAGAAG GTGAAGATGA TGGACAGAGA 
AGCTCCACAG AACAAAGTGG CGAAGCCCAG GGAGAAGCAG CCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759079.1
CDS	443..919
Translation	MTQCHYKNHLSSFVGSVSSRIPRCGKLQYKLGSLSSSRYTVSSRIPYHKAGWSTSFLAWNKEDSKHLRTIKYVSLLCGYLLAMTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	XM_008760857.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760857.2

ORF Insert Sequence:

ATGACTCAAT GTCACTATAA AAATCATCTT AGCAGCTTCG TAGGCTCAGT TTCTTCGAGG 
ATACCCCGAT GTGGAAAGCT TCAGTATAAG CTAGGAAGCC TTTCTTCTTC CAGATACACA 
GTGTCCTCTA GAATTCCCTA TCACAAAGCA GGCTGGTCCA CTAGCTTTTT GGCATGGAAC 
AAGGAAGACA GCAAACATCT AAGGACAATT AAATATGTTT CCCTGCTATG TGGATATTTG 
TTAGCAATGA CTGATGTGGA AACTACGTAT GCAGATTTTA TTGCCTCAGG AAGAACAGGT 
AGAAGAAATG CAATACATGA TATCTTGGTT TCCTCTGCAA GTGGCAACAG CAATGAATTA 
GCCTTGAAAT TAGCAGGTCT TGATATTAAC AAGACAGAAG GTGAAGATGA TGGACAGAGA 
AGCTCCACAG AACAAAGTGG CGAAGCCCAG GGAGAAGCAG CCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The testis-specific C?2 subunit of PKA is kinetically indistinguishable from the common C?1 subunit of PKA.
BMC biochemistry1240(2011 Aug)
Vetter MM,Zenn HM,M?ndez E,van den Boom H,Herberg FW,Sk?lhegg BS

Characterization of PKIgamma, a novel isoform of the protein kinase inhibitor of cAMP-dependent protein kinase.
The Journal of biological chemistry272(29)18169-78(1997 Jul)
Collins SP,Uhler MD

The expression and intracellular distribution of the heat-stable protein kinase inhibitor is cell cycle regulated.
The Journal of biological chemistry270(5)2041-6(1995 Feb)
Wen W,Taylor SS,Meinkoth JL

Glutamic acid 203 of the cAMP-dependent protein kinase catalytic subunit participates in the inhibition by two isoforms of the protein kinase inhibitor.
The Journal of biological chemistry269(3)2316-23(1994 Jan)
Baude EJ,Dignam SS,Olsen SR,Reimann EM,Uhler MD

Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase.
Science (New York, N.Y.)253(5018)407-14(1991 Jul)
Knighton DR,Zheng JH,Ten Eyck LF,Ashford VA,Xuong NH,Taylor SS,Sowadski JM