LOC7484301 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7484301
Entrez Gene ID	7484301
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_568103
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002316533.2	XP_002316569.2	hypothetical protein
XM_024581513.1	XP_024437281.1	TMV resistance protein N
XM_024581512.1	XP_024437280.1	TMV resistance protein N

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7484301 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7484301 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa25320	XM_002316533.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0011s01860g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OPa439871	XM_024581513.1 Latest version!	Populus trichocarpa TMV resistance protein N (LOC7484301), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa439871	XM_024581512.1 Latest version!	Populus trichocarpa TMV resistance protein N (LOC7484301), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7484301 ( XM_002316533.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002316569.2 Populus trichocarpa hypothetical protein (POPTR_0011s01860g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa25320
Clone ID Related Accession (Same CDS sequence)	XM_002316533.2
Accession Version	XM_002316533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008477). On Dec 16, 2013 this sequence version replaced XM_002316533.1. COMPLETENESS: incomplete on the 5' end.

ATGGAAGATG ATCATGCAGA AGTTGTCTGT ACCAATTTAA TGGTTCGCAG CAAGCGTTGC 
AGGCTTAACT TCTTTCAACA ATGGCTTTCT GACTTTTTCG ATGGAGAAAA ATTACAAACT 
GGCCAAACAA GTTTGTTTCC CATCCTCAAA ACAGATGCTT TTAGAAAGAT GCCAGATATA 
AAATTTCTCC AACTAAATTA CACGAAGTTC TATGGAAGTT TTGAGCACTT CCCTAAGAAT 
TTGATATGGT TATGTTGGCA TGGATTCTCT TCCAGATCCA TACCAAATCA CGTATGTTTG 
GAGAAGCTGG TGGTTCTTGA TCTATCCAGA AGCTGTCTAG TTGATGCTTG GAAAGGCAAA 
CCGTTTTTTC CAAAATTGAA AATTCTTGAT CTCCGTCACT CTCATGATCT CATTAGAACC 
CCAGACTTCT CGGGTCTCCC AGCCCTTGAA AAACTAATAC TTGAAGACTG CATCCGTTTG 
GTTCAAATTC ATGAATCTAT TGGTGATTTA CAAAGATTGT TGATCTTAAA TCTAAGAAAT 
TGTACAAGTC TTATGGAGCT TCCAGAAGAA ATGAGTAGAT TGAATTCACT TCAAGAGCTG 
GTTTTAGGTG GTTGCTCAAA TCTTGACAGC TTGAATATGG AGTTAGAGCA TCATCAGTGG 
CGCAGCTTGC TTCAAAGCGA TGAAATTGTT GCAAGTACAT CACACATTAC ATCTCTTCCA 
TTGAAGCTAT TCTTTCCCTC TAGGTTTTCA GCGAGGAAAA TATTGAGATT TACGTTGTTT 
TCGCTGCCAC GCTCCTTGAC GAGACTAGAT TTAAGTGGAA CTCCAATTCG TTTTCTTCCA 
GAAAGCATCA AGGATCTTGG TCCACTCAGA CATCTATATT TAAGAAATTG CAAAATGCTT 
CAGGCACTCT TAGAGCTTCC ATCCCATTTG TGGTCGTTAG ATGTGTCCTT TTGCTATTCA 
CTGCAAAGAC CTGCAAATCC AAACAGTTCG AATAAAGCAG ATGGTTGTGA TCAATTTGTC 
GAGTTCCAAG ATTGGATAAA GCAAGAATTA ATCCAAACGT TTGACTCACA CGTGTTCAGA 
ATAATGGAAA CGGTTTGTGC TCAAATACAG CCATCGAGAT TTCAGATGAC ATTTGTATAT 
GGCAAATTCA ACTTTGTCAA CTTTGTATTT GATGAAGATG AGACGTTAAG GAGGTTTCAT 
GAAGAGGAAG AAGAGGATAA ATGGCTAATT CAGAATGAGT TTAGTGTGAC GTCAGAGTAC 
AGTTGCCATG AAGAAGTTGG TATTGGAATC AGAAACAATA TCAGTGGTCA ATCCTTGAGT 
CGTCAAGGGG TCTTCCCTGC GTGTAACATG CGGAGGTTTC GTGAAATCCA ATCGCTAAGC 
CACTGGAAAT TAGGGGCCAA TGATCCTACA TTTGATAATG GTGATGACGT GAGTATTTCA 
GTGCTTCCAC ATGATCCATC GCTAAGCAAC TGGATTTTCG CCACATATGA TAACGGTGAT 
GACATGAGTA TTTCAGTGCT TCAACATGAG CCAGCTATTC AAATAAGGAC GATTGGTATC 
CAATGGTTGC ATGAAGAGGA AGGAAATGAT AATGATATCC AATCAAAGAA TGAAGATATC 
ACATCCCATT GCAGCAGCTC ATCAAACAGT GAAGTTATCA ATGCCCACAA CAGTAGCGAT 
GAAGATGACG TACTCAAAGT AGGAATAGCT TCTCATATTT TTAGAAATTA TTATTGCCTT 
TCCCATTATA ATCATGATGC CATGGAAGCA AGGATGTGGA CTTTTGAAAA GAAAGTTCCA 
GAGGCAATAG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002316569.2
CDS	1..1818
Translation	MEDDHAEVVCTNLMVRSKRCRLNFFQQWLSDFFDGEKLQTGQTSLFPILKTDAFRKMPDIKFLQLNYTKFYGSFEHFPKNLIWLCWHGFSSRSIPNHVCLEKLVVLDLSRSCLVDAWKGKPFFPKLKILDLRHSHDLIRTPDFSGLPALEKLILEDCIRLVQIHESIGDLQRLLILNLRNCTSLMELPEEMSRLNSLQELVLGGCSNLDSLNMELEHHQWRSLLQSDEIVASTSHITSLPLKLFFPSRFSARKILRFTLFSLPRSLTRLDLSGTPIRFLPESIKDLGPLRHLYLRNCKMLQALLELPSHLWSLDVSFCYSLQRPANPNSSNKADGCDQFVEFQDWIKQELIQTFDSHVFRIMETVCAQIQPSRFQMTFVYGKFNFVNFVFDEDETLRRFHEEEEEDKWLIQNEFSVTSEYSCHEEVGIGIRNNISGQSLSRQGVFPACNMRRFREIQSLSHWKLGANDPTFDNGDDVSISVLPHDPSLSNWIFATYDNGDDMSISVLQHEPAIQIRTIGIQWLHEEEGNDNDIQSKNEDITSHCSSSSNSEVINAHNSSDEDDVLKVGIASHIFRNYYCLSHYNHDAMEARMWTFEKKVPEAIEQ

Target ORF information:

RefSeq Version	XM_002316533.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0011s01860g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002316533.2

ORF Insert Sequence:

ATGGAAGATG ATCATGCAGA AGTTGTCTGT ACCAATTTAA TGGTTCGCAG CAAGCGTTGC 
AGGCTTAACT TCTTTCAACA ATGGCTTTCT GACTTTTTCG ATGGAGAAAA ATTACAAACT 
GGCCAAACAA GTTTGTTTCC CATCCTCAAA ACAGATGCTT TTAGAAAGAT GCCAGATATA 
AAATTTCTCC AACTAAATTA CACGAAGTTC TATGGAAGTT TTGAGCACTT CCCTAAGAAT 
TTGATATGGT TATGTTGGCA TGGATTCTCT TCCAGATCCA TACCAAATCA CGTATGTTTG 
GAGAAGCTGG TGGTTCTTGA TCTATCCAGA AGCTGTCTAG TTGATGCTTG GAAAGGCAAA 
CCGTTTTTTC CAAAATTGAA AATTCTTGAT CTCCGTCACT CTCATGATCT CATTAGAACC 
CCAGACTTCT CGGGTCTCCC AGCCCTTGAA AAACTAATAC TTGAAGACTG CATCCGTTTG 
GTTCAAATTC ATGAATCTAT TGGTGATTTA CAAAGATTGT TGATCTTAAA TCTAAGAAAT 
TGTACAAGTC TTATGGAGCT TCCAGAAGAA ATGAGTAGAT TGAATTCACT TCAAGAGCTG 
GTTTTAGGTG GTTGCTCAAA TCTTGACAGC TTGAATATGG AGTTAGAGCA TCATCAGTGG 
CGCAGCTTGC TTCAAAGCGA TGAAATTGTT GCAAGTACAT CACACATTAC ATCTCTTCCA 
TTGAAGCTAT TCTTTCCCTC TAGGTTTTCA GCGAGGAAAA TATTGAGATT TACGTTGTTT 
TCGCTGCCAC GCTCCTTGAC GAGACTAGAT TTAAGTGGAA CTCCAATTCG TTTTCTTCCA 
GAAAGCATCA AGGATCTTGG TCCACTCAGA CATCTATATT TAAGAAATTG CAAAATGCTT 
CAGGCACTCT TAGAGCTTCC ATCCCATTTG TGGTCGTTAG ATGTGTCCTT TTGCTATTCA 
CTGCAAAGAC CTGCAAATCC AAACAGTTCG AATAAAGCAG ATGGTTGTGA TCAATTTGTC 
GAGTTCCAAG ATTGGATAAA GCAAGAATTA ATCCAAACGT TTGACTCACA CGTGTTCAGA 
ATAATGGAAA CGGTTTGTGC TCAAATACAG CCATCGAGAT TTCAGATGAC ATTTGTATAT 
GGCAAATTCA ACTTTGTCAA CTTTGTATTT GATGAAGATG AGACGTTAAG GAGGTTTCAT 
GAAGAGGAAG AAGAGGATAA ATGGCTAATT CAGAATGAGT TTAGTGTGAC GTCAGAGTAC 
AGTTGCCATG AAGAAGTTGG TATTGGAATC AGAAACAATA TCAGTGGTCA ATCCTTGAGT 
CGTCAAGGGG TCTTCCCTGC GTGTAACATG CGGAGGTTTC GTGAAATCCA ATCGCTAAGC 
CACTGGAAAT TAGGGGCCAA TGATCCTACA TTTGATAATG GTGATGACGT GAGTATTTCA 
GTGCTTCCAC ATGATCCATC GCTAAGCAAC TGGATTTTCG CCACATATGA TAACGGTGAT 
GACATGAGTA TTTCAGTGCT TCAACATGAG CCAGCTATTC AAATAAGGAC GATTGGTATC 
CAATGGTTGC ATGAAGAGGA AGGAAATGAT AATGATATCC AATCAAAGAA TGAAGATATC 
ACATCCCATT GCAGCAGCTC ATCAAACAGT GAAGTTATCA ATGCCCACAA CAGTAGCGAT 
GAAGATGACG TACTCAAAGT AGGAATAGCT TCTCATATTT TTAGAAATTA TTATTGCCTT 
TCCCATTATA ATCATGATGC CATGGAAGCA AGGATGTGGA CTTTTGAAAA GAAAGTTCCA 
GAGGCAATAG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7484301 ( XM_024581513.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024437281.1 Populus trichocarpa TMV resistance protein N (LOC7484301), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa439871
Clone ID Related Accession (Same CDS sequence)	XM_024581513.1 , XM_024581512.1
Accession Version	XM_024581513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	TMV resistance protein N
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037295.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GGAAATATCA AGAATCCTAC TCTTCACGGT TTTCTTATTG TAAATATCAA 
GTGTTCTTGA GTTTTAGAGG TGAAGACACC CGCAAGAACT TTACCGATCA CCTCTACAAG 
GCCCTGGTTG ATGCAGGGTT TCACACATTT AGAGATGATG ATGAAATTCG GAGAGGAAAG 
AATATACAGC TGGAGCTCCA GAAGGCAATA CAACAATCAA AAATAGCGAT AATCGTGTTC 
TCCAAAAACT ATGCTTGGTC GAGATGGTGC CTCGATGAAC TTGTAAAGAT CATGGAACGT 
AATAGGAATG CTGACTGCAT AGTTTTCCCA GTATTCTATC ATGTGGATCC ATCTGAAGTC 
AGAAATCAAA ATGGGAGCTT CGCTGCAGCA TTTGTTGAAC ATGAAAAGCA CTACAAGGAG 
GAGATGGAGC GGGTGAATGG GTGGAGGATT GCTTTGAAGG AAGTTGCAAA TTTAGCTGGA 
ATGGATTTAG GAGATGGGTA CGAGGCACAG TTTGTCCAAT CTATTGTGGA GAATGTCTCA 
AAGAATTTGG ATCCAAAAAT ATTTTATGTC CCCCTTCATT TCATTGGAAG AGATCCTCTA 
GTACAGGATA TCAACTCATG GTTGCAAGAT GGATCCCATG GTGCTGCCAT TGCTTTACTC 
TATGGAATTG GTGGAGTTGG AAAGACAGCC ATAGCTAAGA GTGTTTTTAA CCAGAACTTT 
TATAAATTTG AAGGAAAGAG CTTTCTATCA AATTTTAGGT CAAAGGATAT AGTTTGCCTA 
CAGAGGCAAC TTATTTTTGA CATCCTAAAC AAGACTGTTG AGATAAATGA TGAAGATGAA 
GGAATTCTGA AAATTAAGGA TGCATTATGT TGCAGAAGAA CTCTTATTGT TCTAGATGAT 
GTGGACAAAA GGGACCAATT CAATAAAATC ATTGGCATGC AAAATTGGCT TTGTAAAGGA 
AGTAAAATCA TTGTAACAAC CAGAAATAAG GGTTTGTTTT CAGCTAATGA TATTGAGGGG 
GTCCGGTGCA AAGTTGGACC GCTAGATGAT GAAAAATCAC TTGAGCTTTT TAGTTGGAAT 
GCCTTTGGAC AAGCTGACCC TGTTGATGGT TTTGTAGAAG ACTCTTGGAG AATAGTACAT 
CATTGTAATG GACTTCCATT AGCTCTTCGA GTTATTGGCT CTTCATTGTC CGGAAAAGGA 
AGAGAAATAT GGGAAAGCGC ATTAAAACAA ATGGAAGTGA TTCCAAATTG TGAAGTTCAA 
AAGGTTCTTC GAATAAGTTA CGACTCTCTT GATGATGATT ATCAGAAGGG TTTATTCCTT 
GATATTGCTT GTTTCTTCAA TGGAATGGAT GAGGATGATG CAGTTAGGAT ACTGGATGGG 
CTCGATAAAG GTGCAAGATT TGGGATTGAC AATCTCATTG ATAGATGTCT TGTTGAAATC 
AACAATGATA AAAGGTTGTG GATGCATCAA CTAGTAAGAG ATATGGGAAG GGAAATTGCT 
CGTCAAGAAT CTCCCAAATG TCAAAGAATA TGGCGTCACG AGGATGCTTT TACCGTTTTG 
AAAGGAACTA CTGATGCCGA AAAATTGCGA GGCCTTACCC TGGATATGCA TGCATTAATG 
GAAGATGATC ATGCAGAAGT TGTCTGTACC AATTTAATGG TTCGCAGCAA GCGTTGCAGG 
CTTAACTTCT TTCAACAATG GCTTTCTGAC TTTTTCGATG GAGAAAAATT ACAAACTGGC 
CAAACAAGTT TGTTTCCCAT CCTCAAAACA GATGCTTTTA GAAAGATGCC AGATATAAAA 
TTTCTCCAAC TAAATTACAC GAAGTTCTAT GGAAGTTTTG AGCACTTCCC TAAGAATTTG 
ATATGGTTAT GTTGGCATGG ATTCTCTTCC AGATCCATAC CAAATCACGT ATGTTTGGAG 
AAGCTGGTGG TTCTTGATCT ATCCAGAAGC TGTCTAGTTG ATGCTTGGAA AGGCAAACCG 
TTTTTTCCAA AATTGAAAAT TCTTGATCTC CGTCACTCTC ATGATCTCAT TAGAACCCCA 
GACTTCTCGG GTCTCCCAGC CCTTGAAAAA CTAATACTTG AAGACTGCAT CCGTTTGGTT 
CAAATTCATG AATCTATTGG TGATTTACAA AGATTGTTGA TCTTAAATCT AAGAAATTGT 
ACAAGTCTTA TGGAGCTTCC AGAAGAAATG AGTAGATTGA ATTCACTTCA AGAGCTGGTT 
TTAGGTGGTT GCTCAAATCT TGACAGCTTG AATATGGAGT TAGAGCATCA TCAGTGGCGC 
AGCTTGCTTC AAAGCGATGA AATTGTTGCA AGTACATCAC ACATTACATC TCTTCCATTG 
AAGCTATTCT TTCCCTCTAG GTTTTCAGCG AGGAAAATAT TGAGATTTAC GTTGTTTTCG 
CTGCCACGCT CCTTGACGAG ACTAGATTTA AGTGGAACTC CAATTCGTTT TCTTCCAGAA 
AGCATCAAGG ATCTTGGTCC ACTCAGACAT CTATATTTAA GAAATTGCAA AATGCTTCAG 
GCACTCTTAG AGCTTCCATC CCATTTGTGG TCGTTAGATG TGTCCTTTTG CTATTCACTG 
CAAAGACCTG CAAATCCAAA CTGTTCGAAT AAAGCAGATG GTTGTGATCA ATTTGTCGAG 
TTCCAAGATT GGATAAAGCA AGAATTAATC CAAACGTTTG ACTCACACGT GTTCAGAATA 
ATGGAAACGG TTTGTGCTCA AATACAGCCA TCGAGATTTC AGATGACATT TGTATATGGC 
AAATTCAACT TTGTCAACTT TGTATTTGAT GAAGATGAGA CGTTAAGGAG GTTTCATGAA 
GAGGAAGAAG AGGATAAATG GCTAATTCAG AATGAGTTTA CAGATAACTT TTCATTCAAA 
ATATCCTCAC GTCCTGCGCA CCGGATATGT GGCTTTAATC TGTTCACAAG GTTTAGTGTG 
ACGTCAGAGT ACAGTTGCCA TGAAGAAGTT GGTATTGGAA TCAGAAACAA TATCAGTGGT 
CAATCCTTGA GTCGTCAAGG GGTCTTCCCT GCGTGTAACA TGCGGAGGTT TCGTGAAATC 
CAATCGCTAA GCCACTGGAA ATTAGGGGCC AATGATCCTA CATTTGATAA TGGTGATGAC 
GTGAGTATTT CAGTGCTTCC ACATGATCCA TCGCTAAGCA ACTGGATTTT CGCCACATAT 
GATAACGGTG ATGACATGAG TATTTCAGTG CTTCAACATG AGCCAGCTAT TCAAATAAGG 
ACGATTGGTA TCCAATGGTT GCATGAAGAG GAAGGAAATG ATAATGATAT CCAATCAAAG 
AATGAAGATA TCACATCCCA TTGCAGCAGC TCATCAAACA GTGAAGTTAT CAATGCCCAC 
AACAGTAGCG ATGAAGATGA CGTACTCAAA GTAGGAATAG CTTCTCATAT TTTTAGAAAT 
TATTATTGCC TTTCCCATTA TAATCATGAT GCCATGGAAG CAAGGATGTG GACTTTTGAA 
AAGAAAGTTC CAGAGGCAAT AGAACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024437281.1
CDS	118..3627
Translation	MAAGKYQESYSSRFSYCKYQVFLSFRGEDTRKNFTDHLYKALVDAGFHTFRDDDEIRRGKNIQLELQKAIQQSKIAIIVFSKNYAWSRWCLDELVKIMERNRNADCIVFPVFYHVDPSEVRNQNGSFAAAFVEHEKHYKEEMERVNGWRIALKEVANLAGMDLGDGYEAQFVQSIVENVSKNLDPKIFYVPLHFIGRDPLVQDINSWLQDGSHGAAIALLYGIGGVGKTAIAKSVFNQNFYKFEGKSFLSNFRSKDIVCLQRQLIFDILNKTVEINDEDEGILKIKDALCCRRTLIVLDDVDKRDQFNKIIGMQNWLCKGSKIIVTTRNKGLFSANDIEGVRCKVGPLDDEKSLELFSWNAFGQADPVDGFVEDSWRIVHHCNGLPLALRVIGSSLSGKGREIWESALKQMEVIPNCEVQKVLRISYDSLDDDYQKGLFLDIACFFNGMDEDDAVRILDGLDKGARFGIDNLIDRCLVEINNDKRLWMHQLVRDMGREIARQESPKCQRIWRHEDAFTVLKGTTDAEKLRGLTLDMHALMEDDHAEVVCTNLMVRSKRCRLNFFQQWLSDFFDGEKLQTGQTSLFPILKTDAFRKMPDIKFLQLNYTKFYGSFEHFPKNLIWLCWHGFSSRSIPNHVCLEKLVVLDLSRSCLVDAWKGKPFFPKLKILDLRHSHDLIRTPDFSGLPALEKLILEDCIRLVQIHESIGDLQRLLILNLRNCTSLMELPEEMSRLNSLQELVLGGCSNLDSLNMELEHHQWRSLLQSDEIVASTSHITSLPLKLFFPSRFSARKILRFTLFSLPRSLTRLDLSGTPIRFLPESIKDLGPLRHLYLRNCKMLQALLELPSHLWSLDVSFCYSLQRPANPNCSNKADGCDQFVEFQDWIKQELIQTFDSHVFRIMETVCAQIQPSRFQMTFVYGKFNFVNFVFDEDETLRRFHEEEEEDKWLIQNEFTDNFSFKISSRPAHRICGFNLFTRFSVTSEYSCHEEVGIGIRNNISGQSLSRQGVFPACNMRRFREIQSLSHWKLGANDPTFDNGDDVSISVLPHDPSLSNWIFATYDNGDDMSISVLQHEPAIQIRTIGIQWLHEEEGNDNDIQSKNEDITSHCSSSSNSEVINAHNSSDEDDVLKVGIASHIFRNYYCLSHYNHDAMEARMWTFEKKVPEAIEQ

Target ORF information:

RefSeq Version	XM_024581513.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa TMV resistance protein N (LOC7484301), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024581513.1

ORF Insert Sequence:

ATGGCTGCTG GGAAATATCA AGAATCCTAC TCTTCACGGT TTTCTTATTG TAAATATCAA 
GTGTTCTTGA GTTTTAGAGG TGAAGACACC CGCAAGAACT TTACCGATCA CCTCTACAAG 
GCCCTGGTTG ATGCAGGGTT TCACACATTT AGAGATGATG ATGAAATTCG GAGAGGAAAG 
AATATACAGC TGGAGCTCCA GAAGGCAATA CAACAATCAA AAATAGCGAT AATCGTGTTC 
TCCAAAAACT ATGCTTGGTC GAGATGGTGC CTCGATGAAC TTGTAAAGAT CATGGAACGT 
AATAGGAATG CTGACTGCAT AGTTTTCCCA GTATTCTATC ATGTGGATCC ATCTGAAGTC 
AGAAATCAAA ATGGGAGCTT CGCTGCAGCA TTTGTTGAAC ATGAAAAGCA CTACAAGGAG 
GAGATGGAGC GGGTGAATGG GTGGAGGATT GCTTTGAAGG AAGTTGCAAA TTTAGCTGGA 
ATGGATTTAG GAGATGGGTA CGAGGCACAG TTTGTCCAAT CTATTGTGGA GAATGTCTCA 
AAGAATTTGG ATCCAAAAAT ATTTTATGTC CCCCTTCATT TCATTGGAAG AGATCCTCTA 
GTACAGGATA TCAACTCATG GTTGCAAGAT GGATCCCATG GTGCTGCCAT TGCTTTACTC 
TATGGAATTG GTGGAGTTGG AAAGACAGCC ATAGCTAAGA GTGTTTTTAA CCAGAACTTT 
TATAAATTTG AAGGAAAGAG CTTTCTATCA AATTTTAGGT CAAAGGATAT AGTTTGCCTA 
CAGAGGCAAC TTATTTTTGA CATCCTAAAC AAGACTGTTG AGATAAATGA TGAAGATGAA 
GGAATTCTGA AAATTAAGGA TGCATTATGT TGCAGAAGAA CTCTTATTGT TCTAGATGAT 
GTGGACAAAA GGGACCAATT CAATAAAATC ATTGGCATGC AAAATTGGCT TTGTAAAGGA 
AGTAAAATCA TTGTAACAAC CAGAAATAAG GGTTTGTTTT CAGCTAATGA TATTGAGGGG 
GTCCGGTGCA AAGTTGGACC GCTAGATGAT GAAAAATCAC TTGAGCTTTT TAGTTGGAAT 
GCCTTTGGAC AAGCTGACCC TGTTGATGGT TTTGTAGAAG ACTCTTGGAG AATAGTACAT 
CATTGTAATG GACTTCCATT AGCTCTTCGA GTTATTGGCT CTTCATTGTC CGGAAAAGGA 
AGAGAAATAT GGGAAAGCGC ATTAAAACAA ATGGAAGTGA TTCCAAATTG TGAAGTTCAA 
AAGGTTCTTC GAATAAGTTA CGACTCTCTT GATGATGATT ATCAGAAGGG TTTATTCCTT 
GATATTGCTT GTTTCTTCAA TGGAATGGAT GAGGATGATG CAGTTAGGAT ACTGGATGGG 
CTCGATAAAG GTGCAAGATT TGGGATTGAC AATCTCATTG ATAGATGTCT TGTTGAAATC 
AACAATGATA AAAGGTTGTG GATGCATCAA CTAGTAAGAG ATATGGGAAG GGAAATTGCT 
CGTCAAGAAT CTCCCAAATG TCAAAGAATA TGGCGTCACG AGGATGCTTT TACCGTTTTG 
AAAGGAACTA CTGATGCCGA AAAATTGCGA GGCCTTACCC TGGATATGCA TGCATTAATG 
GAAGATGATC ATGCAGAAGT TGTCTGTACC AATTTAATGG TTCGCAGCAA GCGTTGCAGG 
CTTAACTTCT TTCAACAATG GCTTTCTGAC TTTTTCGATG GAGAAAAATT ACAAACTGGC 
CAAACAAGTT TGTTTCCCAT CCTCAAAACA GATGCTTTTA GAAAGATGCC AGATATAAAA 
TTTCTCCAAC TAAATTACAC GAAGTTCTAT GGAAGTTTTG AGCACTTCCC TAAGAATTTG 
ATATGGTTAT GTTGGCATGG ATTCTCTTCC AGATCCATAC CAAATCACGT ATGTTTGGAG 
AAGCTGGTGG TTCTTGATCT ATCCAGAAGC TGTCTAGTTG ATGCTTGGAA AGGCAAACCG 
TTTTTTCCAA AATTGAAAAT TCTTGATCTC CGTCACTCTC ATGATCTCAT TAGAACCCCA 
GACTTCTCGG GTCTCCCAGC CCTTGAAAAA CTAATACTTG AAGACTGCAT CCGTTTGGTT 
CAAATTCATG AATCTATTGG TGATTTACAA AGATTGTTGA TCTTAAATCT AAGAAATTGT 
ACAAGTCTTA TGGAGCTTCC AGAAGAAATG AGTAGATTGA ATTCACTTCA AGAGCTGGTT 
TTAGGTGGTT GCTCAAATCT TGACAGCTTG AATATGGAGT TAGAGCATCA TCAGTGGCGC 
AGCTTGCTTC AAAGCGATGA AATTGTTGCA AGTACATCAC ACATTACATC TCTTCCATTG 
AAGCTATTCT TTCCCTCTAG GTTTTCAGCG AGGAAAATAT TGAGATTTAC GTTGTTTTCG 
CTGCCACGCT CCTTGACGAG ACTAGATTTA AGTGGAACTC CAATTCGTTT TCTTCCAGAA 
AGCATCAAGG ATCTTGGTCC ACTCAGACAT CTATATTTAA GAAATTGCAA AATGCTTCAG 
GCACTCTTAG AGCTTCCATC CCATTTGTGG TCGTTAGATG TGTCCTTTTG CTATTCACTG 
CAAAGACCTG CAAATCCAAA CTGTTCGAAT AAAGCAGATG GTTGTGATCA ATTTGTCGAG 
TTCCAAGATT GGATAAAGCA AGAATTAATC CAAACGTTTG ACTCACACGT GTTCAGAATA 
ATGGAAACGG TTTGTGCTCA AATACAGCCA TCGAGATTTC AGATGACATT TGTATATGGC 
AAATTCAACT TTGTCAACTT TGTATTTGAT GAAGATGAGA CGTTAAGGAG GTTTCATGAA 
GAGGAAGAAG AGGATAAATG GCTAATTCAG AATGAGTTTA CAGATAACTT TTCATTCAAA 
ATATCCTCAC GTCCTGCGCA CCGGATATGT GGCTTTAATC TGTTCACAAG GTTTAGTGTG 
ACGTCAGAGT ACAGTTGCCA TGAAGAAGTT GGTATTGGAA TCAGAAACAA TATCAGTGGT 
CAATCCTTGA GTCGTCAAGG GGTCTTCCCT GCGTGTAACA TGCGGAGGTT TCGTGAAATC 
CAATCGCTAA GCCACTGGAA ATTAGGGGCC AATGATCCTA CATTTGATAA TGGTGATGAC 
GTGAGTATTT CAGTGCTTCC ACATGATCCA TCGCTAAGCA ACTGGATTTT CGCCACATAT 
GATAACGGTG ATGACATGAG TATTTCAGTG CTTCAACATG AGCCAGCTAT TCAAATAAGG 
ACGATTGGTA TCCAATGGTT GCATGAAGAG GAAGGAAATG ATAATGATAT CCAATCAAAG 
AATGAAGATA TCACATCCCA TTGCAGCAGC TCATCAAACA GTGAAGTTAT CAATGCCCAC 
AACAGTAGCG ATGAAGATGA CGTACTCAAA GTAGGAATAG CTTCTCATAT TTTTAGAAAT 
TATTATTGCC TTTCCCATTA TAATCATGAT GCCATGGAAG CAAGGATGTG GACTTTTGAA 
AAGAAAGTTC CAGAGGCAAT AGAACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7484301 ( XM_024581512.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024437280.1 Populus trichocarpa TMV resistance protein N (LOC7484301), transcript variant X1, mRNA.

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CloneID	OPa439871
Clone ID Related Accession (Same CDS sequence)	XM_024581513.1 , XM_024581512.1
Accession Version	XM_024581512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	TMV resistance protein N
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037295.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GGAAATATCA AGAATCCTAC TCTTCACGGT TTTCTTATTG TAAATATCAA 
GTGTTCTTGA GTTTTAGAGG TGAAGACACC CGCAAGAACT TTACCGATCA CCTCTACAAG 
GCCCTGGTTG ATGCAGGGTT TCACACATTT AGAGATGATG ATGAAATTCG GAGAGGAAAG 
AATATACAGC TGGAGCTCCA GAAGGCAATA CAACAATCAA AAATAGCGAT AATCGTGTTC 
TCCAAAAACT ATGCTTGGTC GAGATGGTGC CTCGATGAAC TTGTAAAGAT CATGGAACGT 
AATAGGAATG CTGACTGCAT AGTTTTCCCA GTATTCTATC ATGTGGATCC ATCTGAAGTC 
AGAAATCAAA ATGGGAGCTT CGCTGCAGCA TTTGTTGAAC ATGAAAAGCA CTACAAGGAG 
GAGATGGAGC GGGTGAATGG GTGGAGGATT GCTTTGAAGG AAGTTGCAAA TTTAGCTGGA 
ATGGATTTAG GAGATGGGTA CGAGGCACAG TTTGTCCAAT CTATTGTGGA GAATGTCTCA 
AAGAATTTGG ATCCAAAAAT ATTTTATGTC CCCCTTCATT TCATTGGAAG AGATCCTCTA 
GTACAGGATA TCAACTCATG GTTGCAAGAT GGATCCCATG GTGCTGCCAT TGCTTTACTC 
TATGGAATTG GTGGAGTTGG AAAGACAGCC ATAGCTAAGA GTGTTTTTAA CCAGAACTTT 
TATAAATTTG AAGGAAAGAG CTTTCTATCA AATTTTAGGT CAAAGGATAT AGTTTGCCTA 
CAGAGGCAAC TTATTTTTGA CATCCTAAAC AAGACTGTTG AGATAAATGA TGAAGATGAA 
GGAATTCTGA AAATTAAGGA TGCATTATGT TGCAGAAGAA CTCTTATTGT TCTAGATGAT 
GTGGACAAAA GGGACCAATT CAATAAAATC ATTGGCATGC AAAATTGGCT TTGTAAAGGA 
AGTAAAATCA TTGTAACAAC CAGAAATAAG GGTTTGTTTT CAGCTAATGA TATTGAGGGG 
GTCCGGTGCA AAGTTGGACC GCTAGATGAT GAAAAATCAC TTGAGCTTTT TAGTTGGAAT 
GCCTTTGGAC AAGCTGACCC TGTTGATGGT TTTGTAGAAG ACTCTTGGAG AATAGTACAT 
CATTGTAATG GACTTCCATT AGCTCTTCGA GTTATTGGCT CTTCATTGTC CGGAAAAGGA 
AGAGAAATAT GGGAAAGCGC ATTAAAACAA ATGGAAGTGA TTCCAAATTG TGAAGTTCAA 
AAGGTTCTTC GAATAAGTTA CGACTCTCTT GATGATGATT ATCAGAAGGG TTTATTCCTT 
GATATTGCTT GTTTCTTCAA TGGAATGGAT GAGGATGATG CAGTTAGGAT ACTGGATGGG 
CTCGATAAAG GTGCAAGATT TGGGATTGAC AATCTCATTG ATAGATGTCT TGTTGAAATC 
AACAATGATA AAAGGTTGTG GATGCATCAA CTAGTAAGAG ATATGGGAAG GGAAATTGCT 
CGTCAAGAAT CTCCCAAATG TCAAAGAATA TGGCGTCACG AGGATGCTTT TACCGTTTTG 
AAAGGAACTA CTGATGCCGA AAAATTGCGA GGCCTTACCC TGGATATGCA TGCATTAATG 
GAAGATGATC ATGCAGAAGT TGTCTGTACC AATTTAATGG TTCGCAGCAA GCGTTGCAGG 
CTTAACTTCT TTCAACAATG GCTTTCTGAC TTTTTCGATG GAGAAAAATT ACAAACTGGC 
CAAACAAGTT TGTTTCCCAT CCTCAAAACA GATGCTTTTA GAAAGATGCC AGATATAAAA 
TTTCTCCAAC TAAATTACAC GAAGTTCTAT GGAAGTTTTG AGCACTTCCC TAAGAATTTG 
ATATGGTTAT GTTGGCATGG ATTCTCTTCC AGATCCATAC CAAATCACGT ATGTTTGGAG 
AAGCTGGTGG TTCTTGATCT ATCCAGAAGC TGTCTAGTTG ATGCTTGGAA AGGCAAACCG 
TTTTTTCCAA AATTGAAAAT TCTTGATCTC CGTCACTCTC ATGATCTCAT TAGAACCCCA 
GACTTCTCGG GTCTCCCAGC CCTTGAAAAA CTAATACTTG AAGACTGCAT CCGTTTGGTT 
CAAATTCATG AATCTATTGG TGATTTACAA AGATTGTTGA TCTTAAATCT AAGAAATTGT 
ACAAGTCTTA TGGAGCTTCC AGAAGAAATG AGTAGATTGA ATTCACTTCA AGAGCTGGTT 
TTAGGTGGTT GCTCAAATCT TGACAGCTTG AATATGGAGT TAGAGCATCA TCAGTGGCGC 
AGCTTGCTTC AAAGCGATGA AATTGTTGCA AGTACATCAC ACATTACATC TCTTCCATTG 
AAGCTATTCT TTCCCTCTAG GTTTTCAGCG AGGAAAATAT TGAGATTTAC GTTGTTTTCG 
CTGCCACGCT CCTTGACGAG ACTAGATTTA AGTGGAACTC CAATTCGTTT TCTTCCAGAA 
AGCATCAAGG ATCTTGGTCC ACTCAGACAT CTATATTTAA GAAATTGCAA AATGCTTCAG 
GCACTCTTAG AGCTTCCATC CCATTTGTGG TCGTTAGATG TGTCCTTTTG CTATTCACTG 
CAAAGACCTG CAAATCCAAA CTGTTCGAAT AAAGCAGATG GTTGTGATCA ATTTGTCGAG 
TTCCAAGATT GGATAAAGCA AGAATTAATC CAAACGTTTG ACTCACACGT GTTCAGAATA 
ATGGAAACGG TTTGTGCTCA AATACAGCCA TCGAGATTTC AGATGACATT TGTATATGGC 
AAATTCAACT TTGTCAACTT TGTATTTGAT GAAGATGAGA CGTTAAGGAG GTTTCATGAA 
GAGGAAGAAG AGGATAAATG GCTAATTCAG AATGAGTTTA CAGATAACTT TTCATTCAAA 
ATATCCTCAC GTCCTGCGCA CCGGATATGT GGCTTTAATC TGTTCACAAG GTTTAGTGTG 
ACGTCAGAGT ACAGTTGCCA TGAAGAAGTT GGTATTGGAA TCAGAAACAA TATCAGTGGT 
CAATCCTTGA GTCGTCAAGG GGTCTTCCCT GCGTGTAACA TGCGGAGGTT TCGTGAAATC 
CAATCGCTAA GCCACTGGAA ATTAGGGGCC AATGATCCTA CATTTGATAA TGGTGATGAC 
GTGAGTATTT CAGTGCTTCC ACATGATCCA TCGCTAAGCA ACTGGATTTT CGCCACATAT 
GATAACGGTG ATGACATGAG TATTTCAGTG CTTCAACATG AGCCAGCTAT TCAAATAAGG 
ACGATTGGTA TCCAATGGTT GCATGAAGAG GAAGGAAATG ATAATGATAT CCAATCAAAG 
AATGAAGATA TCACATCCCA TTGCAGCAGC TCATCAAACA GTGAAGTTAT CAATGCCCAC 
AACAGTAGCG ATGAAGATGA CGTACTCAAA GTAGGAATAG CTTCTCATAT TTTTAGAAAT 
TATTATTGCC TTTCCCATTA TAATCATGAT GCCATGGAAG CAAGGATGTG GACTTTTGAA 
AAGAAAGTTC CAGAGGCAAT AGAACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024437280.1
CDS	172..3681
Translation	MAAGKYQESYSSRFSYCKYQVFLSFRGEDTRKNFTDHLYKALVDAGFHTFRDDDEIRRGKNIQLELQKAIQQSKIAIIVFSKNYAWSRWCLDELVKIMERNRNADCIVFPVFYHVDPSEVRNQNGSFAAAFVEHEKHYKEEMERVNGWRIALKEVANLAGMDLGDGYEAQFVQSIVENVSKNLDPKIFYVPLHFIGRDPLVQDINSWLQDGSHGAAIALLYGIGGVGKTAIAKSVFNQNFYKFEGKSFLSNFRSKDIVCLQRQLIFDILNKTVEINDEDEGILKIKDALCCRRTLIVLDDVDKRDQFNKIIGMQNWLCKGSKIIVTTRNKGLFSANDIEGVRCKVGPLDDEKSLELFSWNAFGQADPVDGFVEDSWRIVHHCNGLPLALRVIGSSLSGKGREIWESALKQMEVIPNCEVQKVLRISYDSLDDDYQKGLFLDIACFFNGMDEDDAVRILDGLDKGARFGIDNLIDRCLVEINNDKRLWMHQLVRDMGREIARQESPKCQRIWRHEDAFTVLKGTTDAEKLRGLTLDMHALMEDDHAEVVCTNLMVRSKRCRLNFFQQWLSDFFDGEKLQTGQTSLFPILKTDAFRKMPDIKFLQLNYTKFYGSFEHFPKNLIWLCWHGFSSRSIPNHVCLEKLVVLDLSRSCLVDAWKGKPFFPKLKILDLRHSHDLIRTPDFSGLPALEKLILEDCIRLVQIHESIGDLQRLLILNLRNCTSLMELPEEMSRLNSLQELVLGGCSNLDSLNMELEHHQWRSLLQSDEIVASTSHITSLPLKLFFPSRFSARKILRFTLFSLPRSLTRLDLSGTPIRFLPESIKDLGPLRHLYLRNCKMLQALLELPSHLWSLDVSFCYSLQRPANPNCSNKADGCDQFVEFQDWIKQELIQTFDSHVFRIMETVCAQIQPSRFQMTFVYGKFNFVNFVFDEDETLRRFHEEEEEDKWLIQNEFTDNFSFKISSRPAHRICGFNLFTRFSVTSEYSCHEEVGIGIRNNISGQSLSRQGVFPACNMRRFREIQSLSHWKLGANDPTFDNGDDVSISVLPHDPSLSNWIFATYDNGDDMSISVLQHEPAIQIRTIGIQWLHEEEGNDNDIQSKNEDITSHCSSSSNSEVINAHNSSDEDDVLKVGIASHIFRNYYCLSHYNHDAMEARMWTFEKKVPEAIEQ

Target ORF information:

RefSeq Version	XM_024581512.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa TMV resistance protein N (LOC7484301), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024581512.1

ORF Insert Sequence:

ATGGCTGCTG GGAAATATCA AGAATCCTAC TCTTCACGGT TTTCTTATTG TAAATATCAA 
GTGTTCTTGA GTTTTAGAGG TGAAGACACC CGCAAGAACT TTACCGATCA CCTCTACAAG 
GCCCTGGTTG ATGCAGGGTT TCACACATTT AGAGATGATG ATGAAATTCG GAGAGGAAAG 
AATATACAGC TGGAGCTCCA GAAGGCAATA CAACAATCAA AAATAGCGAT AATCGTGTTC 
TCCAAAAACT ATGCTTGGTC GAGATGGTGC CTCGATGAAC TTGTAAAGAT CATGGAACGT 
AATAGGAATG CTGACTGCAT AGTTTTCCCA GTATTCTATC ATGTGGATCC ATCTGAAGTC 
AGAAATCAAA ATGGGAGCTT CGCTGCAGCA TTTGTTGAAC ATGAAAAGCA CTACAAGGAG 
GAGATGGAGC GGGTGAATGG GTGGAGGATT GCTTTGAAGG AAGTTGCAAA TTTAGCTGGA 
ATGGATTTAG GAGATGGGTA CGAGGCACAG TTTGTCCAAT CTATTGTGGA GAATGTCTCA 
AAGAATTTGG ATCCAAAAAT ATTTTATGTC CCCCTTCATT TCATTGGAAG AGATCCTCTA 
GTACAGGATA TCAACTCATG GTTGCAAGAT GGATCCCATG GTGCTGCCAT TGCTTTACTC 
TATGGAATTG GTGGAGTTGG AAAGACAGCC ATAGCTAAGA GTGTTTTTAA CCAGAACTTT 
TATAAATTTG AAGGAAAGAG CTTTCTATCA AATTTTAGGT CAAAGGATAT AGTTTGCCTA 
CAGAGGCAAC TTATTTTTGA CATCCTAAAC AAGACTGTTG AGATAAATGA TGAAGATGAA 
GGAATTCTGA AAATTAAGGA TGCATTATGT TGCAGAAGAA CTCTTATTGT TCTAGATGAT 
GTGGACAAAA GGGACCAATT CAATAAAATC ATTGGCATGC AAAATTGGCT TTGTAAAGGA 
AGTAAAATCA TTGTAACAAC CAGAAATAAG GGTTTGTTTT CAGCTAATGA TATTGAGGGG 
GTCCGGTGCA AAGTTGGACC GCTAGATGAT GAAAAATCAC TTGAGCTTTT TAGTTGGAAT 
GCCTTTGGAC AAGCTGACCC TGTTGATGGT TTTGTAGAAG ACTCTTGGAG AATAGTACAT 
CATTGTAATG GACTTCCATT AGCTCTTCGA GTTATTGGCT CTTCATTGTC CGGAAAAGGA 
AGAGAAATAT GGGAAAGCGC ATTAAAACAA ATGGAAGTGA TTCCAAATTG TGAAGTTCAA 
AAGGTTCTTC GAATAAGTTA CGACTCTCTT GATGATGATT ATCAGAAGGG TTTATTCCTT 
GATATTGCTT GTTTCTTCAA TGGAATGGAT GAGGATGATG CAGTTAGGAT ACTGGATGGG 
CTCGATAAAG GTGCAAGATT TGGGATTGAC AATCTCATTG ATAGATGTCT TGTTGAAATC 
AACAATGATA AAAGGTTGTG GATGCATCAA CTAGTAAGAG ATATGGGAAG GGAAATTGCT 
CGTCAAGAAT CTCCCAAATG TCAAAGAATA TGGCGTCACG AGGATGCTTT TACCGTTTTG 
AAAGGAACTA CTGATGCCGA AAAATTGCGA GGCCTTACCC TGGATATGCA TGCATTAATG 
GAAGATGATC ATGCAGAAGT TGTCTGTACC AATTTAATGG TTCGCAGCAA GCGTTGCAGG 
CTTAACTTCT TTCAACAATG GCTTTCTGAC TTTTTCGATG GAGAAAAATT ACAAACTGGC 
CAAACAAGTT TGTTTCCCAT CCTCAAAACA GATGCTTTTA GAAAGATGCC AGATATAAAA 
TTTCTCCAAC TAAATTACAC GAAGTTCTAT GGAAGTTTTG AGCACTTCCC TAAGAATTTG 
ATATGGTTAT GTTGGCATGG ATTCTCTTCC AGATCCATAC CAAATCACGT ATGTTTGGAG 
AAGCTGGTGG TTCTTGATCT ATCCAGAAGC TGTCTAGTTG ATGCTTGGAA AGGCAAACCG 
TTTTTTCCAA AATTGAAAAT TCTTGATCTC CGTCACTCTC ATGATCTCAT TAGAACCCCA 
GACTTCTCGG GTCTCCCAGC CCTTGAAAAA CTAATACTTG AAGACTGCAT CCGTTTGGTT 
CAAATTCATG AATCTATTGG TGATTTACAA AGATTGTTGA TCTTAAATCT AAGAAATTGT 
ACAAGTCTTA TGGAGCTTCC AGAAGAAATG AGTAGATTGA ATTCACTTCA AGAGCTGGTT 
TTAGGTGGTT GCTCAAATCT TGACAGCTTG AATATGGAGT TAGAGCATCA TCAGTGGCGC 
AGCTTGCTTC AAAGCGATGA AATTGTTGCA AGTACATCAC ACATTACATC TCTTCCATTG 
AAGCTATTCT TTCCCTCTAG GTTTTCAGCG AGGAAAATAT TGAGATTTAC GTTGTTTTCG 
CTGCCACGCT CCTTGACGAG ACTAGATTTA AGTGGAACTC CAATTCGTTT TCTTCCAGAA 
AGCATCAAGG ATCTTGGTCC ACTCAGACAT CTATATTTAA GAAATTGCAA AATGCTTCAG 
GCACTCTTAG AGCTTCCATC CCATTTGTGG TCGTTAGATG TGTCCTTTTG CTATTCACTG 
CAAAGACCTG CAAATCCAAA CTGTTCGAAT AAAGCAGATG GTTGTGATCA ATTTGTCGAG 
TTCCAAGATT GGATAAAGCA AGAATTAATC CAAACGTTTG ACTCACACGT GTTCAGAATA 
ATGGAAACGG TTTGTGCTCA AATACAGCCA TCGAGATTTC AGATGACATT TGTATATGGC 
AAATTCAACT TTGTCAACTT TGTATTTGAT GAAGATGAGA CGTTAAGGAG GTTTCATGAA 
GAGGAAGAAG AGGATAAATG GCTAATTCAG AATGAGTTTA CAGATAACTT TTCATTCAAA 
ATATCCTCAC GTCCTGCGCA CCGGATATGT GGCTTTAATC TGTTCACAAG GTTTAGTGTG 
ACGTCAGAGT ACAGTTGCCA TGAAGAAGTT GGTATTGGAA TCAGAAACAA TATCAGTGGT 
CAATCCTTGA GTCGTCAAGG GGTCTTCCCT GCGTGTAACA TGCGGAGGTT TCGTGAAATC 
CAATCGCTAA GCCACTGGAA ATTAGGGGCC AATGATCCTA CATTTGATAA TGGTGATGAC 
GTGAGTATTT CAGTGCTTCC ACATGATCCA TCGCTAAGCA ACTGGATTTT CGCCACATAT 
GATAACGGTG ATGACATGAG TATTTCAGTG CTTCAACATG AGCCAGCTAT TCAAATAAGG 
ACGATTGGTA TCCAATGGTT GCATGAAGAG GAAGGAAATG ATAATGATAT CCAATCAAAG 
AATGAAGATA TCACATCCCA TTGCAGCAGC TCATCAAACA GTGAAGTTAT CAATGCCCAC 
AACAGTAGCG ATGAAGATGA CGTACTCAAA GTAGGAATAG CTTCTCATAT TTTTAGAAAT 
TATTATTGCC TTTCCCATTA TAATCATGAT GCCATGGAAG CAAGGATGTG GACTTTTGAA 
AAGAAAGTTC CAGAGGCAAT AGAACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7484301 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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