rbm20 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	rbm20
Entrez Gene ID	103465883
Full Name	RNA binding motif protein 20
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008411197.2	XP_008409419.1	RNA-binding protein 20 isoform X2
XM_008411124.2	XP_008409346.1	RNA-binding protein 20 isoform X1

Pathways from BioSystems
Gene Ontology

The following rbm20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rbm20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo166880	XM_008411124.2 Latest version!	Poecilia reticulata RNA binding motif protein 20 (rbm20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo166881	XM_008411197.2 Latest version!	Poecilia reticulata RNA binding motif protein 20 (rbm20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rbm20 ( XM_008411124.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409346.1 Poecilia reticulata RNA binding motif protein 20 (rbm20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo166880
Clone ID Related Accession (Same CDS sequence)	XM_008411124.2
Accession Version	XM_008411124.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	RNA-binding protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008411124.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAA AAGGGCACGG CGGCGGCGCG TACGTCCCTG AGATGAACGA AGCACTGCAG 
AGCATTATAT CACCGCCGTT GGACAGCACA GAGAAGAAGG GTCTTCTGGT GGCTGCCCAG 
CTGCCAGGCA ATGTGCAAGC TGGACCTCAG CTGCCGAACC AGTTCCTCCT GACCCCAGCA 
AGCCTTCAGC TTGCTCAGCT CCAGGCCCAG CTCACCCTCC ATCGCCTCAA ACTGGCTCAG 
GGAGGAAACA CGGCCAACGC CGCCAGCATT CTCAACCAGT TGATTTCCAA TGTCAACATG 
TCCCAGCCCC TGTTGAATCA ATTAAGGAGT TCGTCTTTGG TCGGGAATCC ACAGGGGGCC 
TTCCCCACTG GGGTGATTGG GTTCCCCTCC TCAAATTCAG CCGTTGGGTC GTTGGTGGGT 
GGGGCATTCA ACCAAAACTC AGGGAATGTG CGACTGGATC ATGCTTGTGG AGGGGCTGGG 
AAGGGGCAAC AAGGTGGGGA GTACGGAAAA AATCCTGGAT CAACTTACTC TTCTGACACC 
GACAGACGTC TTCAGTACAG CTTATCTGGA GGAACATCTG CTGACGGACA GTACGCGGGG 
ATTAAATCTC AGGCCAAGAA CATGACCAAT GTTGGCTTTC ACAGAGATTT CTACGGGCAT 
GAATTGCAGG GACAACCAGT TGGGTTTAGC TCCAATGAGC CAGTGAACTC CACCGCACAT 
AAGGAGCAAT GGAAAGGCCA TAACAACTTA AACCAAACTG GAAAGGTGAA CATGGCTTCA 
AACCCTCCCA CAGCATGGCC TTCAGCTGGG CAGCCCATTT GGTCCAGAAC AGAACTGTAC 
AACCCAGAAG AGCCGACCCC CGAGCCCAAG CTGAACTGTA GCGGGGTTTC CTCTTTCGGA 
TCCAGTGGCC CGCAGGGCTT CGGAAGCTAC CAGAACCTGC ATGGGGGCGA GGAAACCCTG 
TCGTCAGGTA CCAGGACACT CCAGGCCCAT CAAGTCAGTG ACTTTTATGG CATTASGCCT 
TCCCAGCTGC CACATCAGTG CAGCATCTGT GAAAAAAAGG TCTACAACCT CAAGGACTGG 
GACCAGCATG TGAAGGGGAA ACTACATCTG CAGAATCAAA CTTTGTACGC CACCGACAGC 
TCAGCGGCGG CGGCGGCCGG AGCCCCTCAG TACGCCGTCG GCAGGCCGTG TGATGGAGGG 
ACGAACCCAG GAGGGACGAA CCCCACAGTC GGCTCCGCCG CAAGTCAAGA TGTGTCGTCA 
GGAACCAAAG CCTCATACCT GCCAGCAGCA GCCATGAAGA CGTATCCCAC AGCAGACGCA 
GCGTTTACCC CCCACCAGCC GGAGTCAAAG CCGTTTCCAC CCAGGAAGGC CTCGGCCGGC 
CGCGTCGTTC ACATCTGCAA CCTGCCCGAA GGCAGCTGCA CGGAGAACGA CGTCATCAAC 
CTGGGAATAC CGTTCGGGAA GGTCACCAAC TACATCCTGA TGCGCTCAAC CCATCAGGCC 
TTTCTGGAGA TGGCGTATGT AGAGGCAGCT CAGGCCATGG TTCAATACTA TCAACTGACT 
CCGGCCACGA TTAACGGTCA GAAACTCCTC ATACGGATGT CTAAGAGGTA CAAGGAGCTG 
CAGCTCAAGA AACCTGGTAA AGATGTTCAG TCAATCATCC AGGATATTAC CTCACAGCAG 
GAAAGGGACA AAATGCAAGA ACTTGAACAC TACCTTCCAG ACAGAGCGCG GTCTCGCAGC 
CCCATCAGCC GCTCCCTGAG TCCTCATTCC CACAGTCCCA GTTTCACGTC GTGCAGCTCT 
GCCCACAGCC CCCCGGCGGC TCCGTGCCGG GGTCAGGACA GGGGCAGCAA CGGCCTGAGC 
CCTCACCGGG GCTCCAGAGA CTGGATATCG CACTTGAGAC GAGGGGAGGA TGAGAGGGAG 
CGGGACGGTC CCTGGAGGAA CGGCGGCAGC GGCGGCGGTG GCAGCATGGA CGACAGTCGA 
CCCAACGGGA GGACGGCCGA CCGGTGGAAG AGCTACCACA AACACTTGGG TCACTTGAGG 
TCAACAGACG AACGCGGAAG CGGCGAAGCG ACAACGACGA GGAGCAGCAG AGACTGGCAC 
CCGCGGGACA GCCCCCTGGG CTCGTCTTTC AACTCCTACA GGAGCATGGA CGAAGATTTC 
AGCAGGACAT CTCACTATCG GCATGACGCA AAGCCGAGGA GGAGGGACGG CGACCACCCC 
GTCCGGTCGA GACACTCCGA GTTTGAGACG ACGAACGAGC CGCGGTGTCG AACGCCGGAG 
GACAAGAGGC GGAGCAGAAA CCCGAGCAGG AGGCACGCCG AGAAACAGGA GAGAGAAGCT 
GCTCCCGAAA ACACTGACGC CGCAGCCAAA GAAAAATCTG TTCTGCCACA ACAAAGCAAC 
AAGTCAAGAG AGGCGGCTGA AAATGACAAG GACGGAGAAA CTGAGAATGT GCTGGAAAGT 
GCCGAGGACA CGGATGAAGA ATGTTGGTAT CCCAAAAACA TGGAGGAGCT GGTCACGGTG 
GATGAAGTGG GAGGGGAGGA CGACTGCATC CTCGAGCCAG ATCTCTCTGA GCTCGAGGAG 
TTTGCTTCCT GTCGCAAACA GCCGATAGCG GAGCAACCGC AGCAGAAGCC CACGTCTCCA 
TCGACTACAG CAGTGGAGGA GCACAAAGAA TCCGGCGAGA AACCTAAACG GGAGGACTCT 
GTTGAGGAGA TGGGGGGCGA GGCGTCAGCA TCCAAAGTTG AGAAAGCAGA GGATCAAATC 
GCCGCACCAA GTACTGAAAC CGAGAAAGTG AATTTAGAGA CGACAGAGCA AAAAGTGTCG 
GACATCAGTG GCTTCCCCAG TGAGGAGTTC AAAGCGGCGC TAGAGGAGAC GTGCTTGGAG 
AGCGCCAAGC TTCCCAACAA CACGGCGCCG GAGGAGCCAA TGGAAAATCA TGTCTGTTTG 
TTAGACGACA GCAGAAGCCA GGAAGAAACA CAGACTCCAG ATAAAATCCA TCTGGAAGTT 
CAGCTGAAGG ACGTCAGTCT GAAAAGAGAG ATCGAAGCCC CCTCCTCATC TCTAGAGCAG 
GACAAAGCTG TCAGTGAACA CAGCATTCCT TTGGGAGTGG AGTTCATTGA GCCAAGAACA 
GGCTTCTACT GCAAACTGTG TGGCTTGTTC TACAGCAGCG AGGACACTGC AAAAACCACT 
CACTGCCGCA GCACCGTACA CTACAGAAAC CTGCAGAAAT ACCTGTCCCA GCTGGCAGAG 
AACGGCCTGT CGAGCGCGCT GGCTGAACCC TCCGCGGCGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409346.1
CDS	428..3712
Translation	MLGKGHGGGAYVPEMNEALQSIISPPLDSTEKKGLLVAAQLPGNVQAGPQLPNQFLLTPASLQLAQLQAQLTLHRLKLAQGGNTANAASILNQLISNVNMSQPLLNQLRSSSLVGNPQGAFPTGVIGFPSSNSAVGSLVGGAFNQNSGNVRLDHACGGAGKGQQGGEYGKNPGSTYSSDTDRRLQYSLSGGTSADGQYAGIKSQAKNMTNVGFHRDFYGHELQGQPVGFSSNEPVNSTAHKEQWKGHNNLNQTGKVNMASNPPTAWPSAGQPIWSRTELYNPEEPTPEPKLNCSGVSSFGSSGPQGFGSYQNLHGGEETLSSGTRTLQAHQVSDFYGInullPSQLPHQCSICEKKVYNLKDWDQHVKGKLHLQNQTLYATDSSAAAAAGAPQYAVGRPCDGGTNPGGTNPTVGSAASQDVSSGTKASYLPAAAMKTYPTADAAFTPHQPESKPFPPRKASAGRVVHICNLPEGSCTENDVINLGIPFGKVTNYILMRSTHQAFLEMAYVEAAQAMVQYYQLTPATINGQKLLIRMSKRYKELQLKKPGKDVQSIIQDITSQQERDKMQELEHYLPDRARSRSPISRSLSPHSHSPSFTSCSSAHSPPAAPCRGQDRGSNGLSPHRGSRDWISHLRRGEDERERDGPWRNGGSGGGGSMDDSRPNGRTADRWKSYHKHLGHLRSTDERGSGEATTTRSSRDWHPRDSPLGSSFNSYRSMDEDFSRTSHYRHDAKPRRRDGDHPVRSRHSEFETTNEPRCRTPEDKRRSRNPSRRHAEKQEREAAPENTDAAAKEKSVLPQQSNKSREAAENDKDGETENVLESAEDTDEECWYPKNMEELVTVDEVGGEDDCILEPDLSELEEFASCRKQPIAEQPQQKPTSPSTTAVEEHKESGEKPKREDSVEEMGGEASASKVEKAEDQIAAPSTETEKVNLETTEQKVSDISGFPSEEFKAALEETCLESAKLPNNTAPEEPMENHVCLLDDSRSQEETQTPDKIHLEVQLKDVSLKREIEAPSSSLEQDKAVSEHSIPLGVEFIEPRTGFYCKLCGLFYSSEDTAKTTHCRSTVHYRNLQKYLSQLAENGLSSALAEPSAAH

Target ORF information:

RefSeq Version	XM_008411124.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata RNA binding motif protein 20 (rbm20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008411124.2

ORF Insert Sequence:

ATGCTGGGAA AAGGGCACGG CGGCGGCGCG TACGTCCCTG AGATGAACGA AGCACTGCAG 
AGCATTATAT CACCGCCGTT GGACAGCACA GAGAAGAAGG GTCTTCTGGT GGCTGCCCAG 
CTGCCAGGCA ATGTGCAAGC TGGACCTCAG CTGCCGAACC AGTTCCTCCT GACCCCAGCA 
AGCCTTCAGC TTGCTCAGCT CCAGGCCCAG CTCACCCTCC ATCGCCTCAA ACTGGCTCAG 
GGAGGAAACA CGGCCAACGC CGCCAGCATT CTCAACCAGT TGATTTCCAA TGTCAACATG 
TCCCAGCCCC TGTTGAATCA ATTAAGGAGT TCGTCTTTGG TCGGGAATCC ACAGGGGGCC 
TTCCCCACTG GGGTGATTGG GTTCCCCTCC TCAAATTCAG CCGTTGGGTC GTTGGTGGGT 
GGGGCATTCA ACCAAAACTC AGGGAATGTG CGACTGGATC ATGCTTGTGG AGGGGCTGGG 
AAGGGGCAAC AAGGTGGGGA GTACGGAAAA AATCCTGGAT CAACTTACTC TTCTGACACC 
GACAGACGTC TTCAGTACAG CTTATCTGGA GGAACATCTG CTGACGGACA GTACGCGGGG 
ATTAAATCTC AGGCCAAGAA CATGACCAAT GTTGGCTTTC ACAGAGATTT CTACGGGCAT 
GAATTGCAGG GACAACCAGT TGGGTTTAGC TCCAATGAGC CAGTGAACTC CACCGCACAT 
AAGGAGCAAT GGAAAGGCCA TAACAACTTA AACCAAACTG GAAAGGTGAA CATGGCTTCA 
AACCCTCCCA CAGCATGGCC TTCAGCTGGG CAGCCCATTT GGTCCAGAAC AGAACTGTAC 
AACCCAGAAG AGCCGACCCC CGAGCCCAAG CTGAACTGTA GCGGGGTTTC CTCTTTCGGA 
TCCAGTGGCC CGCAGGGCTT CGGAAGCTAC CAGAACCTGC ATGGGGGCGA GGAAACCCTG 
TCGTCAGGTA CCAGGACACT CCAGGCCCAT CAAGTCAGTG ACTTTTATGG CATTASGCCT 
TCCCAGCTGC CACATCAGTG CAGCATCTGT GAAAAAAAGG TCTACAACCT CAAGGACTGG 
GACCAGCATG TGAAGGGGAA ACTACATCTG CAGAATCAAA CTTTGTACGC CACCGACAGC 
TCAGCGGCGG CGGCGGCCGG AGCCCCTCAG TACGCCGTCG GCAGGCCGTG TGATGGAGGG 
ACGAACCCAG GAGGGACGAA CCCCACAGTC GGCTCCGCCG CAAGTCAAGA TGTGTCGTCA 
GGAACCAAAG CCTCATACCT GCCAGCAGCA GCCATGAAGA CGTATCCCAC AGCAGACGCA 
GCGTTTACCC CCCACCAGCC GGAGTCAAAG CCGTTTCCAC CCAGGAAGGC CTCGGCCGGC 
CGCGTCGTTC ACATCTGCAA CCTGCCCGAA GGCAGCTGCA CGGAGAACGA CGTCATCAAC 
CTGGGAATAC CGTTCGGGAA GGTCACCAAC TACATCCTGA TGCGCTCAAC CCATCAGGCC 
TTTCTGGAGA TGGCGTATGT AGAGGCAGCT CAGGCCATGG TTCAATACTA TCAACTGACT 
CCGGCCACGA TTAACGGTCA GAAACTCCTC ATACGGATGT CTAAGAGGTA CAAGGAGCTG 
CAGCTCAAGA AACCTGGTAA AGATGTTCAG TCAATCATCC AGGATATTAC CTCACAGCAG 
GAAAGGGACA AAATGCAAGA ACTTGAACAC TACCTTCCAG ACAGAGCGCG GTCTCGCAGC 
CCCATCAGCC GCTCCCTGAG TCCTCATTCC CACAGTCCCA GTTTCACGTC GTGCAGCTCT 
GCCCACAGCC CCCCGGCGGC TCCGTGCCGG GGTCAGGACA GGGGCAGCAA CGGCCTGAGC 
CCTCACCGGG GCTCCAGAGA CTGGATATCG CACTTGAGAC GAGGGGAGGA TGAGAGGGAG 
CGGGACGGTC CCTGGAGGAA CGGCGGCAGC GGCGGCGGTG GCAGCATGGA CGACAGTCGA 
CCCAACGGGA GGACGGCCGA CCGGTGGAAG AGCTACCACA AACACTTGGG TCACTTGAGG 
TCAACAGACG AACGCGGAAG CGGCGAAGCG ACAACGACGA GGAGCAGCAG AGACTGGCAC 
CCGCGGGACA GCCCCCTGGG CTCGTCTTTC AACTCCTACA GGAGCATGGA CGAAGATTTC 
AGCAGGACAT CTCACTATCG GCATGACGCA AAGCCGAGGA GGAGGGACGG CGACCACCCC 
GTCCGGTCGA GACACTCCGA GTTTGAGACG ACGAACGAGC CGCGGTGTCG AACGCCGGAG 
GACAAGAGGC GGAGCAGAAA CCCGAGCAGG AGGCACGCCG AGAAACAGGA GAGAGAAGCT 
GCTCCCGAAA ACACTGACGC CGCAGCCAAA GAAAAATCTG TTCTGCCACA ACAAAGCAAC 
AAGTCAAGAG AGGCGGCTGA AAATGACAAG GACGGAGAAA CTGAGAATGT GCTGGAAAGT 
GCCGAGGACA CGGATGAAGA ATGTTGGTAT CCCAAAAACA TGGAGGAGCT GGTCACGGTG 
GATGAAGTGG GAGGGGAGGA CGACTGCATC CTCGAGCCAG ATCTCTCTGA GCTCGAGGAG 
TTTGCTTCCT GTCGCAAACA GCCGATAGCG GAGCAACCGC AGCAGAAGCC CACGTCTCCA 
TCGACTACAG CAGTGGAGGA GCACAAAGAA TCCGGCGAGA AACCTAAACG GGAGGACTCT 
GTTGAGGAGA TGGGGGGCGA GGCGTCAGCA TCCAAAGTTG AGAAAGCAGA GGATCAAATC 
GCCGCACCAA GTACTGAAAC CGAGAAAGTG AATTTAGAGA CGACAGAGCA AAAAGTGTCG 
GACATCAGTG GCTTCCCCAG TGAGGAGTTC AAAGCGGCGC TAGAGGAGAC GTGCTTGGAG 
AGCGCCAAGC TTCCCAACAA CACGGCGCCG GAGGAGCCAA TGGAAAATCA TGTCTGTTTG 
TTAGACGACA GCAGAAGCCA GGAAGAAACA CAGACTCCAG ATAAAATCCA TCTGGAAGTT 
CAGCTGAAGG ACGTCAGTCT GAAAAGAGAG ATCGAAGCCC CCTCCTCATC TCTAGAGCAG 
GACAAAGCTG TCAGTGAACA CAGCATTCCT TTGGGAGTGG AGTTCATTGA GCCAAGAACA 
GGCTTCTACT GCAAACTGTG TGGCTTGTTC TACAGCAGCG AGGACACTGC AAAAACCACT 
CACTGCCGCA GCACCGTACA CTACAGAAAC CTGCAGAAAT ACCTGTCCCA GCTGGCAGAG 
AACGGCCTGT CGAGCGCGCT GGCTGAACCC TCCGCGGCGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rbm20 ( XM_008411197.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409419.1 Poecilia reticulata RNA binding motif protein 20 (rbm20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo166881
Clone ID Related Accession (Same CDS sequence)	XM_008411197.2
Accession Version	XM_008411197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	RNA-binding protein 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024331.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008411197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATA AACTCTGGCA AACTGCAGAA AGAAACAGAT GCACCAGTCC AAGGAAAAAG 
ACCATTATAT CACCGCCGTT GGACAGCACA GAGAAGAAGG GTCTTCTGGT GGCTGCCCAG 
CTGCCAGGCA ATGTGCAAGC TGGACCTCAG CTGCCGAACC AGTTCCTCCT GACCCCAGCA 
AGCCTTCAGC TTGCTCAGCT CCAGGCCCAG CTCACCCTCC ATCGCCTCAA ACTGGCTCAG 
GGAGGAAACA CGGCCAACGC CGCCAGCATT CTCAACCAGT TGATTTCCAA TGTCAACATG 
TCCCAGCCCC TGTTGAATCA ATTAAGGAGT TCGTCTTTGG TCGGGAATCC ACAGGGGGCC 
TTCCCCACTG GGGTGATTGG GTTCCCCTCC TCAAATTCAG CCGTTGGGTC GTTGGTGGGT 
GGGGCATTCA ACCAAAACTC AGGGAATGTG CGACTGGATC ATGCTTGTGG AGGGGCTGGG 
AAGGGGCAAC AAGGTGGGGA GTACGGAAAA AATCCTGGAT CAACTTACTC TTCTGACACC 
GACAGACGTC TTCAGTACAG CTTATCTGGA GGAACATCTG CTGACGGACA GTACGCGGGG 
ATTAAATCTC AGGCCAAGAA CATGACCAAT GTTGGCTTTC ACAGAGATTT CTACGGGCAT 
GAATTGCAGG GACAACCAGT TGGGTTTAGC TCCAATGAGC CAGTGAACTC CACCGCACAT 
AAGGAGCAAT GGAAAGGCCA TAACAACTTA AACCAAACTG GAAAGGTGAA CATGGCTTCA 
AACCCTCCCA CAGCATGGCC TTCAGCTGGG CAGCCCATTT GGTCCAGAAC AGAACTGTAC 
AACCCAGAAG AGCCGACCCC CGAGCCCAAG CTGAACTGTA GCGGGGTTTC CTCTTTCGGA 
TCCAGTGGCC CGCAGGGCTT CGGAAGCTAC CAGAACCTGC ATGGGGGCGA GGAAACCCTG 
TCGTCAGGTA CCAGGACACT CCAGGCCCAT CAAGTCAGTG ACTTTTATGG CATTASGCCT 
TCCCAGCTGC CACATCAGTG CAGCATCTGT GAAAAAAAGG TCTACAACCT CAAGGACTGG 
GACCAGCATG TGAAGGGGAA ACTACATCTG CAGAATCAAA CTTTGTACGC CACCGACAGC 
TCAGCGGCGG CGGCGGCCGG AGCCCCTCAG TACGCCGTCG GCAGGCCGTG TGATGGAGGG 
ACGAACCCAG GAGGGACGAA CCCCACAGTC GGCTCCGCCG CAAGTCAAGA TGTGTCGTCA 
GGAACCAAAG CCTCATACCT GCCAGCAGCA GCCATGAAGA CGTATCCCAC AGCAGACGCA 
GCGTTTACCC CCCACCAGCC GGAGTCAAAG CCGTTTCCAC CCAGGAAGGC CTCGGCCGGC 
CGCGTCGTTC ACATCTGCAA CCTGCCCGAA GGCAGCTGCA CGGAGAACGA CGTCATCAAC 
CTGGGAATAC CGTTCGGGAA GGTCACCAAC TACATCCTGA TGCGCTCAAC CCATCAGGCC 
TTTCTGGAGA TGGCGTATGT AGAGGCAGCT CAGGCCATGG TTCAATACTA TCAACTGACT 
CCGGCCACGA TTAACGGTCA GAAACTCCTC ATACGGATGT CTAAGAGGTA CAAGGAGCTG 
CAGCTCAAGA AACCTGGTAA AGATGTTCAG TCAATCATCC AGGATATTAC CTCACAGCAG 
GAAAGGGACA AAATGCAAGA ACTTGAACAC TACCTTCCAG ACAGAGCGCG GTCTCGCAGC 
CCCATCAGCC GCTCCCTGAG TCCTCATTCC CACAGTCCCA GTTTCACGTC GTGCAGCTCT 
GCCCACAGCC CCCCGGCGGC TCCGTGCCGG GGTCAGGACA GGGGCAGCAA CGGCCTGAGC 
CCTCACCGGG GCTCCAGAGA CTGGATATCG CACTTGAGAC GAGGGGAGGA TGAGAGGGAG 
CGGGACGGTC CCTGGAGGAA CGGCGGCAGC GGCGGCGGTG GCAGCATGGA CGACAGTCGA 
CCCAACGGGA GGACGGCCGA CCGGTGGAAG AGCTACCACA AACACTTGGG TCACTTGAGG 
TCAACAGACG AACGCGGAAG CGGCGAAGCG ACAACGACGA GGAGCAGCAG AGACTGGCAC 
CCGCGGGACA GCCCCCTGGG CTCGTCTTTC AACTCCTACA GGAGCATGGA CGAAGATTTC 
AGCAGGACAT CTCACTATCG GCATGACGCA AAGCCGAGGA GGAGGGACGG CGACCACCCC 
GTCCGGTCGA GACACTCCGA GTTTGAGACG ACGAACGAGC CGCGGTGTCG AACGCCGGAG 
GACAAGAGGC GGAGCAGAAA CCCGAGCAGG AGGCACGCCG AGAAACAGGA GAGAGAAGCT 
GCTCCCGAAA ACACTGACGC CGCAGCCAAA GAAAAATCTG TTCTGCCACA ACAAAGCAAC 
AAGTCAAGAG AGGCGGCTGA AAATGACAAG GACGGAGAAA CTGAGAATGT GCTGGAAAGT 
GCCGAGGACA CGGATGAAGA ATGTTGGTAT CCCAAAAACA TGGAGGAGCT GGTCACGGTG 
GATGAAGTGG GAGGGGAGGA CGACTGCATC CTCGAGCCAG ATCTCTCTGA GCTCGAGGAG 
TTTGCTTCCT GTCGCAAACA GCCGATAGCG GAGCAACCGC AGCAGAAGCC CACGTCTCCA 
TCGACTACAG CAGTGGAGGA GCACAAAGAA TCCGGCGAGA AACCTAAACG GGAGGACTCT 
GTTGAGGAGA TGGGGGGCGA GGCGTCAGCA TCCAAAGTTG AGAAAGCAGA GGATCAAATC 
GCCGCACCAA GTACTGAAAC CGAGAAAGTG AATTTAGAGA CGACAGAGCA AAAAGTGTCG 
GACATCAGTG GCTTCCCCAG TGAGGAGTTC AAAGCGGCGC TAGAGGAGAC GTGCTTGGAG 
AGCGCCAAGC TTCCCAACAA CACGGCGCCG GAGGAGCCAA TGGAAAATCA TGTCTGTTTG 
TTAGACGACA GCAGAAGCCA GGAAGAAACA CAGACTCCAG ATAAAATCCA TCTGGAAGTT 
CAGCTGAAGG ACGTCAGTCT GAAAAGAGAG ATCGAAGCCC CCTCCTCATC TCTAGAGCAG 
GACAAAGCTG TCAGTGAACA CAGCATTCCT TTGGGAGTGG AGTTCATTGA GCCAAGAACA 
GGCTTCTACT GCAAACTGTG TGGCTTGTTC TACAGCAGCG AGGACACTGC AAAAACCACT 
CACTGCCGCA GCACCGTACA CTACAGAAAC CTGCAGAAAT ACCTGTCCCA GCTGGCAGAG 
AACGGCCTGT CGAGCGCGCT GGCTGAACCC TCCGCGGCGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409419.1
CDS	5..3289
Translation	MDHKLWQTAERNRCTSPRKKTIISPPLDSTEKKGLLVAAQLPGNVQAGPQLPNQFLLTPASLQLAQLQAQLTLHRLKLAQGGNTANAASILNQLISNVNMSQPLLNQLRSSSLVGNPQGAFPTGVIGFPSSNSAVGSLVGGAFNQNSGNVRLDHACGGAGKGQQGGEYGKNPGSTYSSDTDRRLQYSLSGGTSADGQYAGIKSQAKNMTNVGFHRDFYGHELQGQPVGFSSNEPVNSTAHKEQWKGHNNLNQTGKVNMASNPPTAWPSAGQPIWSRTELYNPEEPTPEPKLNCSGVSSFGSSGPQGFGSYQNLHGGEETLSSGTRTLQAHQVSDFYGInullPSQLPHQCSICEKKVYNLKDWDQHVKGKLHLQNQTLYATDSSAAAAAGAPQYAVGRPCDGGTNPGGTNPTVGSAASQDVSSGTKASYLPAAAMKTYPTADAAFTPHQPESKPFPPRKASAGRVVHICNLPEGSCTENDVINLGIPFGKVTNYILMRSTHQAFLEMAYVEAAQAMVQYYQLTPATINGQKLLIRMSKRYKELQLKKPGKDVQSIIQDITSQQERDKMQELEHYLPDRARSRSPISRSLSPHSHSPSFTSCSSAHSPPAAPCRGQDRGSNGLSPHRGSRDWISHLRRGEDERERDGPWRNGGSGGGGSMDDSRPNGRTADRWKSYHKHLGHLRSTDERGSGEATTTRSSRDWHPRDSPLGSSFNSYRSMDEDFSRTSHYRHDAKPRRRDGDHPVRSRHSEFETTNEPRCRTPEDKRRSRNPSRRHAEKQEREAAPENTDAAAKEKSVLPQQSNKSREAAENDKDGETENVLESAEDTDEECWYPKNMEELVTVDEVGGEDDCILEPDLSELEEFASCRKQPIAEQPQQKPTSPSTTAVEEHKESGEKPKREDSVEEMGGEASASKVEKAEDQIAAPSTETEKVNLETTEQKVSDISGFPSEEFKAALEETCLESAKLPNNTAPEEPMENHVCLLDDSRSQEETQTPDKIHLEVQLKDVSLKREIEAPSSSLEQDKAVSEHSIPLGVEFIEPRTGFYCKLCGLFYSSEDTAKTTHCRSTVHYRNLQKYLSQLAENGLSSALAEPSAAH

Target ORF information:

RefSeq Version	XM_008411197.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata RNA binding motif protein 20 (rbm20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008411197.2

ORF Insert Sequence:

ATGGATCATA AACTCTGGCA AACTGCAGAA AGAAACAGAT GCACCAGTCC AAGGAAAAAG 
ACCATTATAT CACCGCCGTT GGACAGCACA GAGAAGAAGG GTCTTCTGGT GGCTGCCCAG 
CTGCCAGGCA ATGTGCAAGC TGGACCTCAG CTGCCGAACC AGTTCCTCCT GACCCCAGCA 
AGCCTTCAGC TTGCTCAGCT CCAGGCCCAG CTCACCCTCC ATCGCCTCAA ACTGGCTCAG 
GGAGGAAACA CGGCCAACGC CGCCAGCATT CTCAACCAGT TGATTTCCAA TGTCAACATG 
TCCCAGCCCC TGTTGAATCA ATTAAGGAGT TCGTCTTTGG TCGGGAATCC ACAGGGGGCC 
TTCCCCACTG GGGTGATTGG GTTCCCCTCC TCAAATTCAG CCGTTGGGTC GTTGGTGGGT 
GGGGCATTCA ACCAAAACTC AGGGAATGTG CGACTGGATC ATGCTTGTGG AGGGGCTGGG 
AAGGGGCAAC AAGGTGGGGA GTACGGAAAA AATCCTGGAT CAACTTACTC TTCTGACACC 
GACAGACGTC TTCAGTACAG CTTATCTGGA GGAACATCTG CTGACGGACA GTACGCGGGG 
ATTAAATCTC AGGCCAAGAA CATGACCAAT GTTGGCTTTC ACAGAGATTT CTACGGGCAT 
GAATTGCAGG GACAACCAGT TGGGTTTAGC TCCAATGAGC CAGTGAACTC CACCGCACAT 
AAGGAGCAAT GGAAAGGCCA TAACAACTTA AACCAAACTG GAAAGGTGAA CATGGCTTCA 
AACCCTCCCA CAGCATGGCC TTCAGCTGGG CAGCCCATTT GGTCCAGAAC AGAACTGTAC 
AACCCAGAAG AGCCGACCCC CGAGCCCAAG CTGAACTGTA GCGGGGTTTC CTCTTTCGGA 
TCCAGTGGCC CGCAGGGCTT CGGAAGCTAC CAGAACCTGC ATGGGGGCGA GGAAACCCTG 
TCGTCAGGTA CCAGGACACT CCAGGCCCAT CAAGTCAGTG ACTTTTATGG CATTASGCCT 
TCCCAGCTGC CACATCAGTG CAGCATCTGT GAAAAAAAGG TCTACAACCT CAAGGACTGG 
GACCAGCATG TGAAGGGGAA ACTACATCTG CAGAATCAAA CTTTGTACGC CACCGACAGC 
TCAGCGGCGG CGGCGGCCGG AGCCCCTCAG TACGCCGTCG GCAGGCCGTG TGATGGAGGG 
ACGAACCCAG GAGGGACGAA CCCCACAGTC GGCTCCGCCG CAAGTCAAGA TGTGTCGTCA 
GGAACCAAAG CCTCATACCT GCCAGCAGCA GCCATGAAGA CGTATCCCAC AGCAGACGCA 
GCGTTTACCC CCCACCAGCC GGAGTCAAAG CCGTTTCCAC CCAGGAAGGC CTCGGCCGGC 
CGCGTCGTTC ACATCTGCAA CCTGCCCGAA GGCAGCTGCA CGGAGAACGA CGTCATCAAC 
CTGGGAATAC CGTTCGGGAA GGTCACCAAC TACATCCTGA TGCGCTCAAC CCATCAGGCC 
TTTCTGGAGA TGGCGTATGT AGAGGCAGCT CAGGCCATGG TTCAATACTA TCAACTGACT 
CCGGCCACGA TTAACGGTCA GAAACTCCTC ATACGGATGT CTAAGAGGTA CAAGGAGCTG 
CAGCTCAAGA AACCTGGTAA AGATGTTCAG TCAATCATCC AGGATATTAC CTCACAGCAG 
GAAAGGGACA AAATGCAAGA ACTTGAACAC TACCTTCCAG ACAGAGCGCG GTCTCGCAGC 
CCCATCAGCC GCTCCCTGAG TCCTCATTCC CACAGTCCCA GTTTCACGTC GTGCAGCTCT 
GCCCACAGCC CCCCGGCGGC TCCGTGCCGG GGTCAGGACA GGGGCAGCAA CGGCCTGAGC 
CCTCACCGGG GCTCCAGAGA CTGGATATCG CACTTGAGAC GAGGGGAGGA TGAGAGGGAG 
CGGGACGGTC CCTGGAGGAA CGGCGGCAGC GGCGGCGGTG GCAGCATGGA CGACAGTCGA 
CCCAACGGGA GGACGGCCGA CCGGTGGAAG AGCTACCACA AACACTTGGG TCACTTGAGG 
TCAACAGACG AACGCGGAAG CGGCGAAGCG ACAACGACGA GGAGCAGCAG AGACTGGCAC 
CCGCGGGACA GCCCCCTGGG CTCGTCTTTC AACTCCTACA GGAGCATGGA CGAAGATTTC 
AGCAGGACAT CTCACTATCG GCATGACGCA AAGCCGAGGA GGAGGGACGG CGACCACCCC 
GTCCGGTCGA GACACTCCGA GTTTGAGACG ACGAACGAGC CGCGGTGTCG AACGCCGGAG 
GACAAGAGGC GGAGCAGAAA CCCGAGCAGG AGGCACGCCG AGAAACAGGA GAGAGAAGCT 
GCTCCCGAAA ACACTGACGC CGCAGCCAAA GAAAAATCTG TTCTGCCACA ACAAAGCAAC 
AAGTCAAGAG AGGCGGCTGA AAATGACAAG GACGGAGAAA CTGAGAATGT GCTGGAAAGT 
GCCGAGGACA CGGATGAAGA ATGTTGGTAT CCCAAAAACA TGGAGGAGCT GGTCACGGTG 
GATGAAGTGG GAGGGGAGGA CGACTGCATC CTCGAGCCAG ATCTCTCTGA GCTCGAGGAG 
TTTGCTTCCT GTCGCAAACA GCCGATAGCG GAGCAACCGC AGCAGAAGCC CACGTCTCCA 
TCGACTACAG CAGTGGAGGA GCACAAAGAA TCCGGCGAGA AACCTAAACG GGAGGACTCT 
GTTGAGGAGA TGGGGGGCGA GGCGTCAGCA TCCAAAGTTG AGAAAGCAGA GGATCAAATC 
GCCGCACCAA GTACTGAAAC CGAGAAAGTG AATTTAGAGA CGACAGAGCA AAAAGTGTCG 
GACATCAGTG GCTTCCCCAG TGAGGAGTTC AAAGCGGCGC TAGAGGAGAC GTGCTTGGAG 
AGCGCCAAGC TTCCCAACAA CACGGCGCCG GAGGAGCCAA TGGAAAATCA TGTCTGTTTG 
TTAGACGACA GCAGAAGCCA GGAAGAAACA CAGACTCCAG ATAAAATCCA TCTGGAAGTT 
CAGCTGAAGG ACGTCAGTCT GAAAAGAGAG ATCGAAGCCC CCTCCTCATC TCTAGAGCAG 
GACAAAGCTG TCAGTGAACA CAGCATTCCT TTGGGAGTGG AGTTCATTGA GCCAAGAACA 
GGCTTCTACT GCAAACTGTG TGGCTTGTTC TACAGCAGCG AGGACACTGC AAAAACCACT 
CACTGCCGCA GCACCGTACA CTACAGAAAC CTGCAGAAAT ACCTGTCCCA GCTGGCAGAG 
AACGGCCTGT CGAGCGCGCT GGCTGAACCC TCCGCGGCGC ACTGA