bbox1 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbox1
Entrez Gene ID	103462718
Full Name	gamma-butyrobetaine hydroxylase 1
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008405679.2	XP_008403901.1	gamma-butyrobetaine dioxygenase

Pathways from BioSystems
Gene Ontology

The following bbox1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbox1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo192001	XM_008405679.2 Latest version!	Poecilia reticulata gamma-butyrobetaine hydroxylase 1 (bbox1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbox1 ( XM_008405679.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008403901.1 Poecilia reticulata gamma-butyrobetaine hydroxylase 1 (bbox1), mRNA.

Price & Availability↑

CloneID	OPo192001
Clone ID Related Accession (Same CDS sequence)	XM_008405679.2
Accession Version	XM_008405679.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	gamma-butyrobetaine dioxygenase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024333.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008405679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCCA AGACCACTAA TTTTTTTTGC AGGCACGGCC GACCTTTCAC CTACTTCCCC 
TCTCAAGCTA ACAGCTGCTG CGTTGCTAAA GACAACAACC GGTCGGCGTT TACAAAATAC 
AGTACTGGTG ATAAAGCTTG GGAAGAGCAT TCAGAAATGT GGATGAGTAC ACTTGCACGT 
TTTACTCGTC CCGTTTTTCA AAGGAGCCCG TATCCAGTGG TCTGCAAGGC AATGAAAGCT 
TGCTGTAGGA TTGAGCTGCC TGCTCCAAGT TTCTACATGA CTCCAGTACA TTTTCGCAGA 
CATCAAACCC TTGGTTCTGC CTCCATGCCT GTGGCTGCTC ACTCAATGAG ACATGTCCGT 
GCTCTGGAGG AGGAGAGGCT GATGGCGGTA GAATGGGAAG ATGGAGGCCA AAGCTTGTAC 
CCATTCACCT GGCTGAGAGA CAACTGCCAG TGTCCGCGCT GCACCCTGCA GTCAGCTGAG 
GCCCGCTTGC TGTTGATGGC TGATCTTGAT ATCCACACCG GAATGGATTC TGTGGAGGTT 
ACCAAAGATG GCAAGGTTTC CATTGTGTGG CCCGACCAGC ACATGAGTGT GTTTGATGCA 
GAGTGGCTGA AGAAACGCTG TTTTTCTGAT GCTGCCAGAC AAGTAATGCA GGAGGAGCTG 
TTCCTCAATG AGCGTTACTA TTGGGACGCG ACGCTCAGTA TTCCCCAAGC TGACTTCCAG 
GAAGTTCTCC ATGACGACGG GGCTGCGCTC GCCTGGCTCC TGGCCCTGCG GCGAGTTGGT 
ATCGTGCACC TAAAGGGGGC GCCTGCAGAG CAAGGTCAAG TGGCCAGACT GGCTGAGAGG 
ATTGGATATC TCAGGCTGAC GTTTTACGGG CATACATGGC AGGTCCAGGA CAAATCCATG 
GCAAACAATG TGGCGTACAC TTCAGGGAAG CTGAGCCTGC ATACAGACTA TCCAGCTTTA 
CACTTTGCAC CTGGGGTGCA GTTCCTGCAC TGCATTAACC AGGCGAAGGA AGGAGGGGAG 
AGCGAGGTGG TGGACGGTTT CCACATGGCC GAGCAGCTGC AGCGAGAAGA TCCAGAGGCC 
TTCCGGACGC TCACCTCGCT CCGTGTGGAC TTCACCGACA CCGGGACGGA CTACTGCGAC 
TTCATGCTGC ACTCCAAGAA CCACATCATC GACGTTGACT CCGAGGGAAA AGTCACAAAA 
ATCAACTACA ACAACGCCAC CAGAGACTCT GTGCTGGACC TCCCCTTACA TCAGGTCCAG 
CCCTTCTATA GAGCCCTGAG GGCCTATGTG GACATCATGA ACAGGCCGGA AAACGTGGTC 
ACCTACACCA TGAAGCCAGG TGACATTGTG ACCTTTGACA ACTGGCGTCT GCTGCACGGC 
AGGAAGAGCT ACATCAGCAG GCCAGACAGG CTGCGACACT TGGAGGGTGC CTACTTGGAC 
TGGGACGAAG TGATGTCTCG TCTCCGGATT GTCCGCAGAA ATGTCCACGG GGACGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008403901.1
CDS	16..1515
Translation	MFPKTTNFFCRHGRPFTYFPSQANSCCVAKDNNRSAFTKYSTGDKAWEEHSEMWMSTLARFTRPVFQRSPYPVVCKAMKACCRIELPAPSFYMTPVHFRRHQTLGSASMPVAAHSMRHVRALEEERLMAVEWEDGGQSLYPFTWLRDNCQCPRCTLQSAEARLLLMADLDIHTGMDSVEVTKDGKVSIVWPDQHMSVFDAEWLKKRCFSDAARQVMQEELFLNERYYWDATLSIPQADFQEVLHDDGAALAWLLALRRVGIVHLKGAPAEQGQVARLAERIGYLRLTFYGHTWQVQDKSMANNVAYTSGKLSLHTDYPALHFAPGVQFLHCINQAKEGGESEVVDGFHMAEQLQREDPEAFRTLTSLRVDFTDTGTDYCDFMLHSKNHIIDVDSEGKVTKINYNNATRDSVLDLPLHQVQPFYRALRAYVDIMNRPENVVTYTMKPGDIVTFDNWRLLHGRKSYISRPDRLRHLEGAYLDWDEVMSRLRIVRRNVHGDA

Target ORF information:

RefSeq Version	XM_008405679.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata gamma-butyrobetaine hydroxylase 1 (bbox1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008405679.2

ORF Insert Sequence:

ATGTTCCCCA AGACCACTAA TTTTTTTTGC AGGCACGGCC GACCTTTCAC CTACTTCCCC 
TCTCAAGCTA ACAGCTGCTG CGTTGCTAAA GACAACAACC GGTCGGCGTT TACAAAATAC 
AGTACTGGTG ATAAAGCTTG GGAAGAGCAT TCAGAAATGT GGATGAGTAC ACTTGCACGT 
TTTACTCGTC CCGTTTTTCA AAGGAGCCCG TATCCAGTGG TCTGCAAGGC AATGAAAGCT 
TGCTGTAGGA TTGAGCTGCC TGCTCCAAGT TTCTACATGA CTCCAGTACA TTTTCGCAGA 
CATCAAACCC TTGGTTCTGC CTCCATGCCT GTGGCTGCTC ACTCAATGAG ACATGTCCGT 
GCTCTGGAGG AGGAGAGGCT GATGGCGGTA GAATGGGAAG ATGGAGGCCA AAGCTTGTAC 
CCATTCACCT GGCTGAGAGA CAACTGCCAG TGTCCGCGCT GCACCCTGCA GTCAGCTGAG 
GCCCGCTTGC TGTTGATGGC TGATCTTGAT ATCCACACCG GAATGGATTC TGTGGAGGTT 
ACCAAAGATG GCAAGGTTTC CATTGTGTGG CCCGACCAGC ACATGAGTGT GTTTGATGCA 
GAGTGGCTGA AGAAACGCTG TTTTTCTGAT GCTGCCAGAC AAGTAATGCA GGAGGAGCTG 
TTCCTCAATG AGCGTTACTA TTGGGACGCG ACGCTCAGTA TTCCCCAAGC TGACTTCCAG 
GAAGTTCTCC ATGACGACGG GGCTGCGCTC GCCTGGCTCC TGGCCCTGCG GCGAGTTGGT 
ATCGTGCACC TAAAGGGGGC GCCTGCAGAG CAAGGTCAAG TGGCCAGACT GGCTGAGAGG 
ATTGGATATC TCAGGCTGAC GTTTTACGGG CATACATGGC AGGTCCAGGA CAAATCCATG 
GCAAACAATG TGGCGTACAC TTCAGGGAAG CTGAGCCTGC ATACAGACTA TCCAGCTTTA 
CACTTTGCAC CTGGGGTGCA GTTCCTGCAC TGCATTAACC AGGCGAAGGA AGGAGGGGAG 
AGCGAGGTGG TGGACGGTTT CCACATGGCC GAGCAGCTGC AGCGAGAAGA TCCAGAGGCC 
TTCCGGACGC TCACCTCGCT CCGTGTGGAC TTCACCGACA CCGGGACGGA CTACTGCGAC 
TTCATGCTGC ACTCCAAGAA CCACATCATC GACGTTGACT CCGAGGGAAA AGTCACAAAA 
ATCAACTACA ACAACGCCAC CAGAGACTCT GTGCTGGACC TCCCCTTACA TCAGGTCCAG 
CCCTTCTATA GAGCCCTGAG GGCCTATGTG GACATCATGA ACAGGCCGGA AAACGTGGTC 
ACCTACACCA TGAAGCCAGG TGACATTGTG ACCTTTGACA ACTGGCGTCT GCTGCACGGC 
AGGAAGAGCT ACATCAGCAG GCCAGACAGG CTGCGACACT TGGAGGGTGC CTACTTGGAC 
TGGGACGAAG TGATGTCTCG TCTCCGGATT GTCCGCAGAA ATGTCCACGG GGACGCGTGA