LOC107453431 cDNA ORF clone, Parasteatoda tepidariorum(common house spider)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107453431
Entrez Gene ID	107453431
Full Name	hemicentin-1
Gene Type	protein-coding
Organism	Parasteatoda tepidariorum(common house spider)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016070259.1	XP_015925745.1	hemicentin-1 isoform X1
XM_016070260.1	XP_015925746.1	hemicentin-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC107453431 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107453431 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa137483	XM_016070259.1 Latest version!	Parasteatoda tepidariorum hemicentin-1 (LOC107453431), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPa137484	XM_016070260.1 Latest version!	Parasteatoda tepidariorum hemicentin-1 (LOC107453431), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107453431 ( XM_016070259.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015925745.1 Parasteatoda tepidariorum hemicentin-1 (LOC107453431), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa137483
Clone ID Related Accession (Same CDS sequence)	XM_016070259.1
Accession Version	XM_016070259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	hemicentin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018368549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTTT GTGGAAGCTT GTGGACTCTT GTATTAATTA TAAATTTGAT GTTCTCCTTT 
TATCTTTGCA AAGAGCCAAT ACCCTTGCCA ATACCAGAAT ATGAAGCTGT GGCAGGAGAA 
ATAGGACATT TACCATGCAA TTTTACAATA CCAAGTGATC CATCTTTAGT ATCTTTAATG 
CTCTGGTATA GGGACCAAGA AAGAGCTCCA ATCTATACAG TGGACTTTCG AAAAGGGACT 
AGAGATAACC CAAAACATTT TACAATTCCA GAATATGCAG AGAGATCTTA TTTTGATGTA 
AATATTATAC CACCAACTCT TAGGTTAAAT TCTATTAGTC TTGCAGACGA GGGATATTAC 
AGATGCCATA TAGAGTTTAG GAGATCAAGA ACCATTACAA GAGTCGTTAA TCTAAAAGTG 
ATAACACCAC CTCAATCAGT TGTGATAATG GATGATCGAG GTCAAAGGTT GAAAGATGTA 
GCAGGACCAT ACGATGAAGG CGCTTATTTG TCATTACTTT GTGAAGCAGA AGGAGGCAAT 
CCATCACCCT CAGTAACATG GTGGCGTGAC AAATCATTGT TAGATGACAC GTTCTCTCGA 
ACTTCTCAAA GCTTTGTTAG AAATGAGTAC GTCTTCTTGG AGCTACGAAG ATCAGATCTC 
ATGGCTCAGC TGACGTGCGA AGCATCCAAC ACAAATCTCA CGCAACCGCT GAGGGAGCAA 
ATTAGAATAG ATCTAAATCT TAAACCTATT TATATTCGTA TAACAACTCC ACAAAGGCCT 
TTAAGAGTTG GAGAAGAAAT CCGACTACAT TGTGAAACGT CTGGATCCCG TCCTCCAGCT 
AAACTATCAT GGTGGATAGA TAAAAAACGT GTACAAACTG GTAAAGATTC TATTCCTAAC 
AGTAGAAATA TTACATACAG CACTTTAAGT ATTACACCTA AATCCGATGA TAATGGAAAA 
GCTGTTGTGT GCAAAGCTGA AAATAAAGAT TTGCCACATC CCGTTCTTCA AGACATATGG 
TATTTAAATG TTTTATTTTC TCCAGAAGTG TCATTACAGC TCGGAGCTAA CATAAAATAC 
TCGACCATTA AGGAAGGAAA TGATGTCTAC ATGGAGTGTA TTATCCGGGC AAATCCACCA 
GTTGGAGAAC TAACCTGGTT ATTCGAAAAC AAACCATTCT TTAGTAACGC TTCGGCCGGG 
ATCATCATCT CTAATCAATC TTTAGTTCTG CAAAGGGTGA AAAAAGAGCA TCGAGGGAAA 
TATAGATGTA TTGCTTCTAA TAGTGAAGGC GAAGGGGAAA GCCTGGATTT ATTCTTAGAA 
ATAAGGTATA CTCCAACCTG CAAGCCGTCA AAGAAAAATA TTTATGGTCT AGCTAGAGAA 
GAGAAAGTAA ATATAACGTG TGAAGTAGAT TCGGATCCTG CCGATGTCAC GTTTCGATGG 
GCCTTAAACA ATTCTATTGA AAACGTTGAA CTACACAACT TTAAATCCTC TGGTTCAACA 
AGCATCATAT CTTATATGCC AAAATCTACG ACAGATTATG GATCTGTGTT ATGTTGGGGA 
AGAAATTCAG TTGGGGATCA AAAAGAGCCT TGCTTTTTTA GAATTATACC TGCTGGGCCA 
CCAGATCCCG TACGTAATTG TGTAGTTGTT AACCAGTCCA AATCTTGGCT TCAAATAGAA 
TGCGAAGCTG GTCACGGTAA CGGACTTGAA CAGAGCTTTC ATTTAGATGT ATATAACTCA 
GAAATAAATC ATCTAGAAGT CAATTTAACA TCTGCTACAT CACCTTCGTT TTCAGTCAAA 
GACTTGCCTG CGGGAACTCC ATTTGTACTT GTCGTCTATG CGTCAAATGA AAAAGGGAAA 
AGTAACTCTT TGGCAATCAT GGCTAATACT TTGCACTCTG ATCCACAAAG TAAAGACTCA 
GCCTTTAGTC CGATTGCTGC GATTTTATTG GCCGCGCTGG GTACACTTTT GATGATTGGT 
GTTGTTGGAA TAGCTTTTAT TCAGCTTCGT AGAAGAAGAC CGTTAAAATC TGATATTGAG 
GAAGACAAAT CAAAGATAGA TATGATGCTG AAGAAAGCTT CCGAAGAAAT AGTTGAAACC 
ACAATTGCTT GCCCAGATGT TGTGCCTCCG AATAATACGG TTTATGCTAG TCCAGATTTT 
GAAGGCATTT GTGAAATTAC GAAAGATTCC GATTCATCAA AAATGAAGGA CAATTACAAA 
AACTGTGTTG TATCTCTTAA GAAATCTTGG AGTTTCAAGA AAGGGTTACA AGATATGCCA 
GATGTTGAAG TCACATTACC AGACTTCAAT AATCCATTAG TAAAACATCC GTCATCACCT 
CCGCTAATAG GTGATGTTAC TCTTGAAGAA TGCTTATCAA ATAAGAAAAT TGTGATAAAA 
GTGGAACATT CTCCAGAACC CGATAGGTGT CAAAGCAGAA AGGCTCCATA TGGGATAGAA 
AAGTTAGTTC CTTCCTGTAA ACATGCATTT GTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015925745.1
CDS	229..2727
Translation	MEFCGSLWTLVLIINLMFSFYLCKEPIPLPIPEYEAVAGEIGHLPCNFTIPSDPSLVSLMLWYRDQERAPIYTVDFRKGTRDNPKHFTIPEYAERSYFDVNIIPPTLRLNSISLADEGYYRCHIEFRRSRTITRVVNLKVITPPQSVVIMDDRGQRLKDVAGPYDEGAYLSLLCEAEGGNPSPSVTWWRDKSLLDDTFSRTSQSFVRNEYVFLELRRSDLMAQLTCEASNTNLTQPLREQIRIDLNLKPIYIRITTPQRPLRVGEEIRLHCETSGSRPPAKLSWWIDKKRVQTGKDSIPNSRNITYSTLSITPKSDDNGKAVVCKAENKDLPHPVLQDIWYLNVLFSPEVSLQLGANIKYSTIKEGNDVYMECIIRANPPVGELTWLFENKPFFSNASAGIIISNQSLVLQRVKKEHRGKYRCIASNSEGEGESLDLFLEIRYTPTCKPSKKNIYGLAREEKVNITCEVDSDPADVTFRWALNNSIENVELHNFKSSGSTSIISYMPKSTTDYGSVLCWGRNSVGDQKEPCFFRIIPAGPPDPVRNCVVVNQSKSWLQIECEAGHGNGLEQSFHLDVYNSEINHLEVNLTSATSPSFSVKDLPAGTPFVLVVYASNEKGKSNSLAIMANTLHSDPQSKDSAFSPIAAILLAALGTLLMIGVVGIAFIQLRRRRPLKSDIEEDKSKIDMMLKKASEEIVETTIACPDVVPPNNTVYASPDFEGICEITKDSDSSKMKDNYKNCVVSLKKSWSFKKGLQDMPDVEVTLPDFNNPLVKHPSSPPLIGDVTLEECLSNKKIVIKVEHSPEPDRCQSRKAPYGIEKLVPSCKHAFVC

Target ORF information:

RefSeq Version	XM_016070259.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum hemicentin-1 (LOC107453431), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016070259.1

ORF Insert Sequence:

ATGGAATTTT GTGGAAGCTT GTGGACTCTT GTATTAATTA TAAATTTGAT GTTCTCCTTT 
TATCTTTGCA AAGAGCCAAT ACCCTTGCCA ATACCAGAAT ATGAAGCTGT GGCAGGAGAA 
ATAGGACATT TACCATGCAA TTTTACAATA CCAAGTGATC CATCTTTAGT ATCTTTAATG 
CTCTGGTATA GGGACCAAGA AAGAGCTCCA ATCTATACAG TGGACTTTCG AAAAGGGACT 
AGAGATAACC CAAAACATTT TACAATTCCA GAATATGCAG AGAGATCTTA TTTTGATGTA 
AATATTATAC CACCAACTCT TAGGTTAAAT TCTATTAGTC TTGCAGACGA GGGATATTAC 
AGATGCCATA TAGAGTTTAG GAGATCAAGA ACCATTACAA GAGTCGTTAA TCTAAAAGTG 
ATAACACCAC CTCAATCAGT TGTGATAATG GATGATCGAG GTCAAAGGTT GAAAGATGTA 
GCAGGACCAT ACGATGAAGG CGCTTATTTG TCATTACTTT GTGAAGCAGA AGGAGGCAAT 
CCATCACCCT CAGTAACATG GTGGCGTGAC AAATCATTGT TAGATGACAC GTTCTCTCGA 
ACTTCTCAAA GCTTTGTTAG AAATGAGTAC GTCTTCTTGG AGCTACGAAG ATCAGATCTC 
ATGGCTCAGC TGACGTGCGA AGCATCCAAC ACAAATCTCA CGCAACCGCT GAGGGAGCAA 
ATTAGAATAG ATCTAAATCT TAAACCTATT TATATTCGTA TAACAACTCC ACAAAGGCCT 
TTAAGAGTTG GAGAAGAAAT CCGACTACAT TGTGAAACGT CTGGATCCCG TCCTCCAGCT 
AAACTATCAT GGTGGATAGA TAAAAAACGT GTACAAACTG GTAAAGATTC TATTCCTAAC 
AGTAGAAATA TTACATACAG CACTTTAAGT ATTACACCTA AATCCGATGA TAATGGAAAA 
GCTGTTGTGT GCAAAGCTGA AAATAAAGAT TTGCCACATC CCGTTCTTCA AGACATATGG 
TATTTAAATG TTTTATTTTC TCCAGAAGTG TCATTACAGC TCGGAGCTAA CATAAAATAC 
TCGACCATTA AGGAAGGAAA TGATGTCTAC ATGGAGTGTA TTATCCGGGC AAATCCACCA 
GTTGGAGAAC TAACCTGGTT ATTCGAAAAC AAACCATTCT TTAGTAACGC TTCGGCCGGG 
ATCATCATCT CTAATCAATC TTTAGTTCTG CAAAGGGTGA AAAAAGAGCA TCGAGGGAAA 
TATAGATGTA TTGCTTCTAA TAGTGAAGGC GAAGGGGAAA GCCTGGATTT ATTCTTAGAA 
ATAAGGTATA CTCCAACCTG CAAGCCGTCA AAGAAAAATA TTTATGGTCT AGCTAGAGAA 
GAGAAAGTAA ATATAACGTG TGAAGTAGAT TCGGATCCTG CCGATGTCAC GTTTCGATGG 
GCCTTAAACA ATTCTATTGA AAACGTTGAA CTACACAACT TTAAATCCTC TGGTTCAACA 
AGCATCATAT CTTATATGCC AAAATCTACG ACAGATTATG GATCTGTGTT ATGTTGGGGA 
AGAAATTCAG TTGGGGATCA AAAAGAGCCT TGCTTTTTTA GAATTATACC TGCTGGGCCA 
CCAGATCCCG TACGTAATTG TGTAGTTGTT AACCAGTCCA AATCTTGGCT TCAAATAGAA 
TGCGAAGCTG GTCACGGTAA CGGACTTGAA CAGAGCTTTC ATTTAGATGT ATATAACTCA 
GAAATAAATC ATCTAGAAGT CAATTTAACA TCTGCTACAT CACCTTCGTT TTCAGTCAAA 
GACTTGCCTG CGGGAACTCC ATTTGTACTT GTCGTCTATG CGTCAAATGA AAAAGGGAAA 
AGTAACTCTT TGGCAATCAT GGCTAATACT TTGCACTCTG ATCCACAAAG TAAAGACTCA 
GCCTTTAGTC CGATTGCTGC GATTTTATTG GCCGCGCTGG GTACACTTTT GATGATTGGT 
GTTGTTGGAA TAGCTTTTAT TCAGCTTCGT AGAAGAAGAC CGTTAAAATC TGATATTGAG 
GAAGACAAAT CAAAGATAGA TATGATGCTG AAGAAAGCTT CCGAAGAAAT AGTTGAAACC 
ACAATTGCTT GCCCAGATGT TGTGCCTCCG AATAATACGG TTTATGCTAG TCCAGATTTT 
GAAGGCATTT GTGAAATTAC GAAAGATTCC GATTCATCAA AAATGAAGGA CAATTACAAA 
AACTGTGTTG TATCTCTTAA GAAATCTTGG AGTTTCAAGA AAGGGTTACA AGATATGCCA 
GATGTTGAAG TCACATTACC AGACTTCAAT AATCCATTAG TAAAACATCC GTCATCACCT 
CCGCTAATAG GTGATGTTAC TCTTGAAGAA TGCTTATCAA ATAAGAAAAT TGTGATAAAA 
GTGGAACATT CTCCAGAACC CGATAGGTGT CAAAGCAGAA AGGCTCCATA TGGGATAGAA 
AAGTTAGTTC CTTCCTGTAA ACATGCATTT GTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107453431 ( XM_016070260.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015925746.1 Parasteatoda tepidariorum hemicentin-1 (LOC107453431), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa137484
Clone ID Related Accession (Same CDS sequence)	XM_016070260.1
Accession Version	XM_016070260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	hemicentin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018368549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTTT GTGGAAGCTT GTGGACTCTT GTATTAATTA TAAATTTGAT GTTCTCCTTT 
TATCTTTGCA AAGAGCCAAT ACCCTTGCCA ATACCAGAAT ATGAAGCTGT GGCAGGAGAA 
ATAGGACATT TACCATGCAA TTTTACAATA CCAAGTGATC CATCTTTAGT ATCTTTAATG 
CTCTGGTATA GGGACCAAGA AAGAGCTCCA ATCTATACAG TGGACTTTCG AAAAGGGACT 
AGAGATAACC CAAAACATTT TACAATTCCA GAATATGCAG AGAGATCTTA TTTTGATGTA 
AATATTATAC CACCAACTCT TAGGTTAAAT TCTATTAGTC TTGCAGACGA GGGATATTAC 
AGATGCCATA TAGAGTTTAG GAGATCAAGA ACCATTACAA GAGTCGTTAA TCTAAAAGTG 
ATAACACCAC CTCAATCAGT TGTGATAATG GATGATCGAG GTCAAAGGTT GAAAGATGTA 
GCAGGACCAT ACGATGAAGG CGCTTATTTG TCATTACTTT GTGAAGCAGA AGGAGGCAAT 
CCATCACCCT CAGTAACATG GTGGCGTGAC AAATCATTGT TAGATGACAC GTTCTCTCGA 
ACTTCTCAAA GCTTTGTTAG AAATGAGTAC GTCTTCTTGG AGCTACGAAG ATCAGATCTC 
ATGGCTCAGC TGACGTGCGA AGCATCCAAC ACAAATCTCA CGCAACCGCT GAGGGAGCAA 
ATTAGAATAG ATCTAAATCT TAAACCTATT TATATTCGTA TAACAACTCC ACAAAGGCCT 
TTAAGAGTTG GAGAAGAAAT CCGACTACAT TGTGAAACGT CTGGATCCCG TCCTCCAGCT 
AAACTATCAT GGTGGATAGA TAAAAAACGT GTACAAACTG GTAAAGATTC TATTCCTAAC 
AGTAGAAATA TTACATACAG CACTTTAAGT ATTACACCTA AATCCGATGA TAATGGAAAA 
GCTGTTGTGT GCAAAGCTGA AAATAAAGAT TTGCCACATC CCGTTCTTCA AGACATATGG 
TATTTAAATG TTTTATTTTC TCCAGAAGTG TCATTACAGC TCGGAGCTAA CATAAAATAC 
TCGACCATTA AGGAAGGAAA TGATGTCTAC ATGGAGTGTA TTATCCGGGC AAATCCACCA 
GTTGGAGAAC TAACCTGGTT ATTCGAAAAC AAACCATTCT TTAGTAACGC TTCGGCCGGG 
ATCATCATCT CTAATCAATC TTTAGTTCTG CAAAGGGTGA AAAAAGAGCA TCGAGGGAAA 
TATAGATGTA TTGCTTCTAA TAGTGAAGGC GAAGGGGAAA GCCTGGATTT ATTCTTAGAA 
ATAAGGTATA CTCCAACCTG CAAGCCGTCA AAGAAAAATA TTTATGGTCT AGCTAGAGAA 
GAGAAAGTAA ATATAACGTG TGAAGTAGAT TCGGATCCTG CCGATGTCAC GTTTCGATGG 
GCCTTAAACA ATTCTATTGA AAACGTTGAA CTACACAACT TTAAATCCTC TGGTTCAACA 
AGCATCATAT CTTATATGCC AAAATCTACG ACAGATTATG GATCTGTGTT ATGTTGGGGA 
AGAAATTCAG TTGGGGATCA AAAAGAGCCT TGCTTTTTTA GAATTATACC TGCTGGGCCA 
CCAGATCCCG TACGTAATTG TGTAGTTGTT AACCAGTCCA AATCTTGGCT TCAAATAGAA 
TGCGAAGCTG GTCACGGTAA CGGACTTGAA CAGAGCTTTC ATTTAGATGT ATATAACTCA 
GAAATAAATC ATCTAGAAGT CAATTTAACA TCTGCTACAT CACCTTCGTT TTCAGTCAAA 
GACTTGCCTG CGGGAACTCC ATTTGTACTT GTCGTCTATG CGTCAAATGA AAAAGGGAAA 
AGTAACTCTT TGGCAATCAT GGCTAATACT TTGCACTCTG ATCCACAAAG TAAAGACTCA 
GCCTTTAGTC CGATTGCTGC GATTTTATTG GCCGCGCTGG GTACACTTTT GATGATTGGT 
GTTGTTGGAA TAGCTTTTAT TCAGCTTCGT AGAAGAAGAC CGTTAAAATA TATGATGCTG 
AAGAAAGCTT CCGAAGAAAT AGTTGAAACC ACAATTGCTT GCCCAGATGT TGTGCCTCCG 
AATAATACGG TTTATGCTAG TCCAGATTTT GAAGGCATTT GTGAAATTAC GAAAGATTCC 
GATTCATCAA AAATGAAGGA CAATTACAAA AACTGTGTTG TATCTCTTAA GAAATCTTGG 
AGTTTCAAGA AAGGGTTACA AGATATGCCA GATGTTGAAG TCACATTACC AGACTTCAAT 
AATCCATTAG TAAAACATCC GTCATCACCT CCGCTAATAG GTGATGTTAC TCTTGAAGAA 
TGCTTATCAA ATAAGAAAAT TGTGATAAAA GTGGAACATT CTCCAGAACC CGATAGGTGT 
CAAAGCAGAA AGGCTCCATA TGGGATAGAA AAGTTAGTTC CTTCCTGTAA ACATGCATTT 
GTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015925746.1
CDS	530..2998
Translation	MEFCGSLWTLVLIINLMFSFYLCKEPIPLPIPEYEAVAGEIGHLPCNFTIPSDPSLVSLMLWYRDQERAPIYTVDFRKGTRDNPKHFTIPEYAERSYFDVNIIPPTLRLNSISLADEGYYRCHIEFRRSRTITRVVNLKVITPPQSVVIMDDRGQRLKDVAGPYDEGAYLSLLCEAEGGNPSPSVTWWRDKSLLDDTFSRTSQSFVRNEYVFLELRRSDLMAQLTCEASNTNLTQPLREQIRIDLNLKPIYIRITTPQRPLRVGEEIRLHCETSGSRPPAKLSWWIDKKRVQTGKDSIPNSRNITYSTLSITPKSDDNGKAVVCKAENKDLPHPVLQDIWYLNVLFSPEVSLQLGANIKYSTIKEGNDVYMECIIRANPPVGELTWLFENKPFFSNASAGIIISNQSLVLQRVKKEHRGKYRCIASNSEGEGESLDLFLEIRYTPTCKPSKKNIYGLAREEKVNITCEVDSDPADVTFRWALNNSIENVELHNFKSSGSTSIISYMPKSTTDYGSVLCWGRNSVGDQKEPCFFRIIPAGPPDPVRNCVVVNQSKSWLQIECEAGHGNGLEQSFHLDVYNSEINHLEVNLTSATSPSFSVKDLPAGTPFVLVVYASNEKGKSNSLAIMANTLHSDPQSKDSAFSPIAAILLAALGTLLMIGVVGIAFIQLRRRRPLKYMMLKKASEEIVETTIACPDVVPPNNTVYASPDFEGICEITKDSDSSKMKDNYKNCVVSLKKSWSFKKGLQDMPDVEVTLPDFNNPLVKHPSSPPLIGDVTLEECLSNKKIVIKVEHSPEPDRCQSRKAPYGIEKLVPSCKHAFVC

Target ORF information:

RefSeq Version	XM_016070260.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum hemicentin-1 (LOC107453431), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016070260.1

ORF Insert Sequence:

ATGGAATTTT GTGGAAGCTT GTGGACTCTT GTATTAATTA TAAATTTGAT GTTCTCCTTT 
TATCTTTGCA AAGAGCCAAT ACCCTTGCCA ATACCAGAAT ATGAAGCTGT GGCAGGAGAA 
ATAGGACATT TACCATGCAA TTTTACAATA CCAAGTGATC CATCTTTAGT ATCTTTAATG 
CTCTGGTATA GGGACCAAGA AAGAGCTCCA ATCTATACAG TGGACTTTCG AAAAGGGACT 
AGAGATAACC CAAAACATTT TACAATTCCA GAATATGCAG AGAGATCTTA TTTTGATGTA 
AATATTATAC CACCAACTCT TAGGTTAAAT TCTATTAGTC TTGCAGACGA GGGATATTAC 
AGATGCCATA TAGAGTTTAG GAGATCAAGA ACCATTACAA GAGTCGTTAA TCTAAAAGTG 
ATAACACCAC CTCAATCAGT TGTGATAATG GATGATCGAG GTCAAAGGTT GAAAGATGTA 
GCAGGACCAT ACGATGAAGG CGCTTATTTG TCATTACTTT GTGAAGCAGA AGGAGGCAAT 
CCATCACCCT CAGTAACATG GTGGCGTGAC AAATCATTGT TAGATGACAC GTTCTCTCGA 
ACTTCTCAAA GCTTTGTTAG AAATGAGTAC GTCTTCTTGG AGCTACGAAG ATCAGATCTC 
ATGGCTCAGC TGACGTGCGA AGCATCCAAC ACAAATCTCA CGCAACCGCT GAGGGAGCAA 
ATTAGAATAG ATCTAAATCT TAAACCTATT TATATTCGTA TAACAACTCC ACAAAGGCCT 
TTAAGAGTTG GAGAAGAAAT CCGACTACAT TGTGAAACGT CTGGATCCCG TCCTCCAGCT 
AAACTATCAT GGTGGATAGA TAAAAAACGT GTACAAACTG GTAAAGATTC TATTCCTAAC 
AGTAGAAATA TTACATACAG CACTTTAAGT ATTACACCTA AATCCGATGA TAATGGAAAA 
GCTGTTGTGT GCAAAGCTGA AAATAAAGAT TTGCCACATC CCGTTCTTCA AGACATATGG 
TATTTAAATG TTTTATTTTC TCCAGAAGTG TCATTACAGC TCGGAGCTAA CATAAAATAC 
TCGACCATTA AGGAAGGAAA TGATGTCTAC ATGGAGTGTA TTATCCGGGC AAATCCACCA 
GTTGGAGAAC TAACCTGGTT ATTCGAAAAC AAACCATTCT TTAGTAACGC TTCGGCCGGG 
ATCATCATCT CTAATCAATC TTTAGTTCTG CAAAGGGTGA AAAAAGAGCA TCGAGGGAAA 
TATAGATGTA TTGCTTCTAA TAGTGAAGGC GAAGGGGAAA GCCTGGATTT ATTCTTAGAA 
ATAAGGTATA CTCCAACCTG CAAGCCGTCA AAGAAAAATA TTTATGGTCT AGCTAGAGAA 
GAGAAAGTAA ATATAACGTG TGAAGTAGAT TCGGATCCTG CCGATGTCAC GTTTCGATGG 
GCCTTAAACA ATTCTATTGA AAACGTTGAA CTACACAACT TTAAATCCTC TGGTTCAACA 
AGCATCATAT CTTATATGCC AAAATCTACG ACAGATTATG GATCTGTGTT ATGTTGGGGA 
AGAAATTCAG TTGGGGATCA AAAAGAGCCT TGCTTTTTTA GAATTATACC TGCTGGGCCA 
CCAGATCCCG TACGTAATTG TGTAGTTGTT AACCAGTCCA AATCTTGGCT TCAAATAGAA 
TGCGAAGCTG GTCACGGTAA CGGACTTGAA CAGAGCTTTC ATTTAGATGT ATATAACTCA 
GAAATAAATC ATCTAGAAGT CAATTTAACA TCTGCTACAT CACCTTCGTT TTCAGTCAAA 
GACTTGCCTG CGGGAACTCC ATTTGTACTT GTCGTCTATG CGTCAAATGA AAAAGGGAAA 
AGTAACTCTT TGGCAATCAT GGCTAATACT TTGCACTCTG ATCCACAAAG TAAAGACTCA 
GCCTTTAGTC CGATTGCTGC GATTTTATTG GCCGCGCTGG GTACACTTTT GATGATTGGT 
GTTGTTGGAA TAGCTTTTAT TCAGCTTCGT AGAAGAAGAC CGTTAAAATA TATGATGCTG 
AAGAAAGCTT CCGAAGAAAT AGTTGAAACC ACAATTGCTT GCCCAGATGT TGTGCCTCCG 
AATAATACGG TTTATGCTAG TCCAGATTTT GAAGGCATTT GTGAAATTAC GAAAGATTCC 
GATTCATCAA AAATGAAGGA CAATTACAAA AACTGTGTTG TATCTCTTAA GAAATCTTGG 
AGTTTCAAGA AAGGGTTACA AGATATGCCA GATGTTGAAG TCACATTACC AGACTTCAAT 
AATCCATTAG TAAAACATCC GTCATCACCT CCGCTAATAG GTGATGTTAC TCTTGAAGAA 
TGCTTATCAA ATAAGAAAAT TGTGATAAAA GTGGAACATT CTCCAGAACC CGATAGGTGT 
CAAAGCAGAA AGGCTCCATA TGGGATAGAA AAGTTAGTTC CTTCCTGTAA ACATGCATTT 
GTATGTTAA