KRT25 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	KRT25
Entrez Gene ID	443079
Full Name	keratin 25
Synonyms	CK-25,K25
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009739.1	NP_001009739.1	keratin, type I cytoskeletal 25

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A unique type I keratin intermediate filament gene family is abundantly expressed in the inner root sheaths of sheep and human hair follicles.
Bawden CS, McLaughlan C, Nesci A, Rogers G
The Journal of investigative dermatology116(1)157-66(2001 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KRT25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSm00047	NM_001009739.1 Latest version!	Ovis aries keratin 25 (KRT25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KRT25 ( NM_001009739.1 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001009739.1 Ovis aries keratin 25 (KRT25), mRNA.

Price & Availability↑

CloneID	OSm00047
Clone ID Related Accession (Same CDS sequence)	NM_001009739.1
Accession Version	NM_001009739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Ovis aries(sheep)
Product	keratin, type I cytoskeletal 25
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF227759.1. ##Evidence-Data-START## Transcript exon combination :: AF227759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA104494974, SAMEA104495000 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTTC GACTTCCCAG TGGATCCAGG AGGGCCGGTC CCCGTCCCAC CACGGGCTCA 
CTCAGGCTCT CCGGGGCAGG AGCCAGCTTC GGAGCTGGCA ATGCTTGCAG CATGCCTGGA 
ATTGGAAGCA GTTTCTCATG TGCCTTTGGG AGCAGCTCAT CAGGAGGAAA CGCCTTGGGG 
GGCAATCCCT GTGCTGGCTT TACTATGAAT GAGGGGGGCC TCCTTTCTGG CAATGAGAAG 
GTGACCATGC AGAATCTCAA TGACCGCCTC GCATCCTACC TGGAGAATGT CCGTGCCCTG 
GAGGAGGCCA ACGCTGACCT GGAGCAGAAG ATCAAGGGCT GGTATGAGAA ATTCGGGCCC 
GGTTCTTGCC GTGGTCTTGA TCATGACTAT AGTAGATATC TCCCGATAAT CGAGGATCTT 
AAAAACCAGA TCATTGCTTC CACCACCAGC AATGCCAATG CTGTTCTGCA GATCGATAAT 
GCCAGGCTGA CTGCTGATGT TTTCAGACTC AAGTATGAAA ATGAGCTGGC TCTTCATCAG 
AGCGTAGAGA GCGATGTCAA TGGGCTTCGC AGAGTTCTGG ATGTAATCAC CCTCTGCAGA 
ACAGACCTGG AGATTCAGTA TGAAACCCTG AGTGAAGAGC TGACTTACCT CAAAAAGAAC 
CATAAAGAGG AGATGCAGGT TCTGCAGTGC GCGGCCGGAG GCAACGTGAA CGTGGAGATG 
AACGCAGCGC CCGGCGTGGA CCTCACGGTT CTGCTGAACA ATATGAGAGC CGAGTACGAA 
GCCCTGGCCG AGCAGAACCG CAGGGACGCT GAGGCTTGGT TCAACGAGAA GAGCGCTTCG 
CTGCAACAGC AGATCACTGA GGATGTGGGA GCCACCACCT CAGCCAGGAA TGAGCTGACA 
GAGATGAAGC GCAACCTCCA AACACTGGAA ATTGAACTTC AGTCTCTCCT AGCCACGAAA 
CACTCCCTGG AGTGCTCCTT GACAGAGACG GAGGGCAACT ACTGTGCACA GCTGGCCCAG 
GTCCAGGCTC AGATCGGGGC CCTGGAGGAG CAGCTGCACC AAGTCAGGAC CGAGACCGAG 
GGCCAGAAAC TGGAGTATGA ACAGCTCCTG GACATCAAGG TCCACCTGGA AAAAGAAATC 
GAGACCTACT GTCTCCTCAT TGGTGGAGAT GATGGGGCTT GCAAGTCTGG AGGTTACAAA 
TCTAAAGACT ATGCAGCTGG AAATATGGGA AACCAAATGA AAGATCCAAT CAGAGCCATT 
GTGGTTAAGA AAGTCCTTGA GGAGGTAGAC CAGCGCAGCA AAGTACTTAC CACCAGGCTC 
CACTCCCTGG AAGAGAAATC TCAAAGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009739.1
CDS	25..1377
Translation	MSLRLPSGSRRAGPRPTTGSLRLSGAGASFGAGNACSMPGIGSSFSCAFGSSSSGGNALGGNPCAGFTMNEGGLLSGNEKVTMQNLNDRLASYLENVRALEEANADLEQKIKGWYEKFGPGSCRGLDHDYSRYLPIIEDLKNQIIASTTSNANAVLQIDNARLTADVFRLKYENELALHQSVESDVNGLRRVLDVITLCRTDLEIQYETLSEELTYLKKNHKEEMQVLQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFNEKSASLQQQITEDVGATTSARNELTEMKRNLQTLEIELQSLLATKHSLECSLTETEGNYCAQLAQVQAQIGALEEQLHQVRTETEGQKLEYEQLLDIKVHLEKEIETYCLLIGGDDGACKSGGYKSKDYAAGNMGNQMKDPIRAIVVKKVLEEVDQRSKVLTTRLHSLEEKSQSN

Target ORF information:

RefSeq Version	NM_001009739.1
Organism	Ovis aries(sheep)
Definition	Ovis aries keratin 25 (KRT25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009739.1

ORF Insert Sequence:

ATGTCTCTTC GACTTCCCAG TGGATCCAGG AGGGCCGGTC CCCGTCCCAC CACGGGCTCA 
CTCAGGCTCT CCGGGGCAGG AGCCAGCTTC GGAGCTGGCA ATGCTTGCAG CATGCCTGGA 
ATTGGAAGCA GTTTCTCATG TGCCTTTGGG AGCAGCTCAT CAGGAGGAAA CGCCTTGGGG 
GGCAATCCCT GTGCTGGCTT TACTATGAAT GAGGGGGGCC TCCTTTCTGG CAATGAGAAG 
GTGACCATGC AGAATCTCAA TGACCGCCTC GCATCCTACC TGGAGAATGT CCGTGCCCTG 
GAGGAGGCCA ACGCTGACCT GGAGCAGAAG ATCAAGGGCT GGTATGAGAA ATTCGGGCCC 
GGTTCTTGCC GTGGTCTTGA TCATGACTAT AGTAGATATC TCCCGATAAT CGAGGATCTT 
AAAAACCAGA TCATTGCTTC CACCACCAGC AATGCCAATG CTGTTCTGCA GATCGATAAT 
GCCAGGCTGA CTGCTGATGT TTTCAGACTC AAGTATGAAA ATGAGCTGGC TCTTCATCAG 
AGCGTAGAGA GCGATGTCAA TGGGCTTCGC AGAGTTCTGG ATGTAATCAC CCTCTGCAGA 
ACAGACCTGG AGATTCAGTA TGAAACCCTG AGTGAAGAGC TGACTTACCT CAAAAAGAAC 
CATAAAGAGG AGATGCAGGT TCTGCAGTGC GCGGCCGGAG GCAACGTGAA CGTGGAGATG 
AACGCAGCGC CCGGCGTGGA CCTCACGGTT CTGCTGAACA ATATGAGAGC CGAGTACGAA 
GCCCTGGCCG AGCAGAACCG CAGGGACGCT GAGGCTTGGT TCAACGAGAA GAGCGCTTCG 
CTGCAACAGC AGATCACTGA GGATGTGGGA GCCACCACCT CAGCCAGGAA TGAGCTGACA 
GAGATGAAGC GCAACCTCCA AACACTGGAA ATTGAACTTC AGTCTCTCCT AGCCACGAAA 
CACTCCCTGG AGTGCTCCTT GACAGAGACG GAGGGCAACT ACTGTGCACA GCTGGCCCAG 
GTCCAGGCTC AGATCGGGGC CCTGGAGGAG CAGCTGCACC AAGTCAGGAC CGAGACCGAG 
GGCCAGAAAC TGGAGTATGA ACAGCTCCTG GACATCAAGG TCCACCTGGA AAAAGAAATC 
GAGACCTACT GTCTCCTCAT TGGTGGAGAT GATGGGGCTT GCAAGTCTGG AGGTTACAAA 
TCTAAAGACT ATGCAGCTGG AAATATGGGA AACCAAATGA AAGATCCAAT CAGAGCCATT 
GTGGTTAAGA AAGTCCTTGA GGAGGTAGAC CAGCGCAGCA AAGTACTTAC CACCAGGCTC 
CACTCCCTGG AAGAGAAATC TCAAAGCAAT TAA