KIF20B gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_004020027.3	XP_004020076.1	kinesin-like protein KIF20B isoform X4
XM_012160445.2	XP_012015835.1	kinesin-like protein KIF20B isoform X5
XM_012160446.2	XP_012015836.1	kinesin-like protein KIF20B isoform X5
XM_004020026.4	XP_004020075.2	kinesin-like protein KIF20B isoform X1
XM_012160447.2	XP_012015837.1	kinesin-like protein KIF20B isoform X6
XM_012160444.2	XP_012015834.1	kinesin-like protein KIF20B isoform X5
XM_012160448.2	XP_012015838.1	kinesin-like protein KIF20B isoform X7
XM_012102525.2	XP_011957915.1	kinesin-like protein KIF20B isoform X1
XM_012102526.2	XP_011957916.1	kinesin-like protein KIF20B isoform X1
XM_004020026.3	XP_004020075.1	kinesin-like protein KIF20B isoform X2
XM_012102529.2	XP_011957919.1	kinesin-like protein KIF20B isoform X3
XM_027960158.1	XP_027815959.1	kinesin-like protein KIF20B isoform X1
XM_012102528.2	XP_011957918.1	kinesin-like protein KIF20B isoform X1
XM_012160449.2	XP_012015839.1	kinesin-like protein KIF20B isoform X8
XM_027960159.1	XP_027815960.1	kinesin-like protein KIF20B isoform X1
XM_027960160.1	XP_027815961.1	kinesin-like protein KIF20B isoform X2
XM_027960161.1	XP_027815962.1	kinesin-like protein KIF20B isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv32671	XM_012160446.2 Latest version!	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv32671	XM_012160444.2 Latest version!	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv32671	XM_012160445.2 Latest version!	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv32672	XM_012160447.2 Latest version!	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv32673	XM_012160448.2 Latest version!	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv32674	XM_012160449.2 Latest version!	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSm73183	XM_027960158.1 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm73183	XM_004020026.4 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm73183	XM_027960159.1 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm73184	XM_027960160.1 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm73185	XM_027960161.1 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm16083	XM_004020026.3 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm16084	XM_004020027.3 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm43701	XM_012102528.2 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm43701	XM_012102526.2 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm43701	XM_012102525.2 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm43702	XM_012102529.2 Latest version!	Ovis aries kinesin family member 20B (KIF20B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

KIF20B ( XM_012160445.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012015835.1 Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OOv32671
Clone ID Related Accession (Same CDS sequence)	XM_012160445.2 , XM_012160446.2 , XM_012160444.2
Accession Version	XM_012160445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	kinesin-like protein KIF20B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012160445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012015835.1
CDS	259..5778
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSRISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTKGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012160445.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012160445.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012160446.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012015836.1 Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X9, mRNA.

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CloneID	OOv32671
Clone ID Related Accession (Same CDS sequence)	XM_012160445.2 , XM_012160446.2 , XM_012160444.2
Accession Version	XM_012160446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	kinesin-like protein KIF20B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012160446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012015836.1
CDS	250..5769
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSRISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTKGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012160446.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012160446.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012160444.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012015834.1 Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X7, mRNA.

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CloneID	OOv32671
Clone ID Related Accession (Same CDS sequence)	XM_012160445.2 , XM_012160446.2 , XM_012160444.2
Accession Version	XM_012160444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	kinesin-like protein KIF20B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012160444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012015834.1
CDS	156..5675
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSRISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTKGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012160444.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012160444.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012160447.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012015837.1 Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X10, mRNA.

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CloneID	OOv32672
Clone ID Related Accession (Same CDS sequence)	XM_012160447.2
Accession Version	XM_012160447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	kinesin-like protein KIF20B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012160447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGGAC 
TTTGTGAAAT TTGAAAATAA GAAGAATGCT ACACCAAGAA TTAATTTGAA GTCCCCTGTT 
TCTGGGCATA GAAATTCTTC TGTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT 
AAGAAAACTT ACTCTTTACG AAGTCACGCA TCCACAAGTG ATGTAAACGG GCCTGCTAAG 
AAAAAAGAAG GAACGCTGCA GAAATTTGGA GACTTTTTAC AACATTCTCC AATTATTCTT 
CAATCAAAAG CAAAGAAGAT AATTGAAACG ATGAGCTCTT CAAAGTTCTC AAATGTAGAA 
GTAAGTAAAG ATAACGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA 
ATTTCGTCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCATAA AGTGAAAGAA 
AGCGATCATC AGATTCTCAA ACGAAGACTT CGAACCAAAA CAATCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012015837.1
CDS	156..5606
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSRISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTKGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012160447.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012160447.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGGAC 
TTTGTGAAAT TTGAAAATAA GAAGAATGCT ACACCAAGAA TTAATTTGAA GTCCCCTGTT 
TCTGGGCATA GAAATTCTTC TGTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT 
AAGAAAACTT ACTCTTTACG AAGTCACGCA TCCACAAGTG ATGTAAACGG GCCTGCTAAG 
AAAAAAGAAG GAACGCTGCA GAAATTTGGA GACTTTTTAC AACATTCTCC AATTATTCTT 
CAATCAAAAG CAAAGAAGAT AATTGAAACG ATGAGCTCTT CAAAGTTCTC AAATGTAGAA 
GTAAGTAAAG ATAACGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA 
ATTTCGTCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCATAA AGTGAAAGAA 
AGCGATCATC AGATTCTCAA ACGAAGACTT CGAACCAAAA CAATCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012160448.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012015838.1 Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X11, mRNA.

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CloneID	OOv32673
Clone ID Related Accession (Same CDS sequence)	XM_012160448.2
Accession Version	XM_012160448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	kinesin-like protein KIF20B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012160448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012015838.1
CDS	156..5555
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSRISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTKGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012160448.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012160448.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGAGT 
AGTGTATTTT CAAGCTGGTG GAAGAACAGA AATAATATTA AACATACTCC TGAGGCTCTC 
TGTCAGGACT TTGTGAAATT TGAAAATAAG AAGAATGCTA CACCAAGAAT TAATTTGAAG 
TCCCCTGTTT CTGGGCATAG AAATTCTTCT GTCAAAAAGG AACAAAAGGT TTCCATACGC 
CCATCATCTA AGAAAACTTA CTCTTTACGA AGTCACGCAT CCACAAGTGA TGTAAACGGG 
CCTGCTAAGA AAAAAGAAGG AACGCTGCAG AAATTTGGAG ACTTTTTACA ACATTCTCCA 
ATTATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACGA TGAGCTCTTC AAAGTTCTCA 
AATGTAGAAG TAAGTAAAGA TAACGTGTCT CGACCAAAAC GGGCCAAACG AAAATTATAC 
ACAAATGAAA TTTCGTCTCC TATTGATATA TCTGGCCAAG TGATTTTGAT GGACCATAAA 
GTGAAAGAAA GCGATCATCA GATTCTCAAA CGAAGACTTC GAACCAAAAC AATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012160449.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012015839.1 Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X12, mRNA.

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CloneID	OOv32674
Clone ID Related Accession (Same CDS sequence)	XM_012160449.2
Accession Version	XM_012160449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	kinesin-like protein KIF20B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012160449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGGAC 
TTTGTGAAAT TTGAAAATAA GAAGAATGCT ACACCAAGAA TTAATTTGAA GTCCCCTGTT 
TCTGGGCATA GAAATTCTTC TGTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT 
AAGAAAACTT ACTCTTTACG AAGTCACGCA TCCACAAGTG ATGTAAACGG GCCTGCTAAG 
AAAAAAGAAG GAACGCTGCA GAAATTTGGA GACTTTTTAC AACATTCTCC AATTATTCTT 
CAATCAAAAG CAAAGAAGAT AATTGAAACG ATGAGCTCTT CAAAGTTCTC AAATGTAGAA 
GTAAGTAAAG ATAACGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA 
ATTTCGTCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCATAA AGTGAAAGAA 
AGCGATCATC AGATTCTCAA ACGAAGACTT CGAACCAAAA CAATCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012015839.1
CDS	156..5486
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSRISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTKGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012160449.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon kinesin family member 20B (KIF20B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012160449.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTTG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTATG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA AAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCAGGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AGTTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAG 
CTAAAAACAC GTTTACTCAC CATTGAGAAT GAACTTAAAA ATGAAAAGGA AGAAAAAACA 
GAATTAAATA AACAGATTGT TAGTTTGCAG CAGGAACTTT CTTCTTCTGA AAAAAAAAGT 
TTAGGCTTTA GTATAGAGGT CCAACAAATT CAGTCGAGTT ATGACAATGT GATTTCTGAA 
TTACATGTGC AGAAAAGTAT AAATCAAGAA CAGAAGGAAA GGATCATAAA ATTGTCAGAG 
GAGATAGAAA CTGCTAGAAG AAACATCACA AATAATGTTT CCCAAATAAA ATTAATGCAG 
GCAAAAATAG ATGAACTGCG TAGACTTGAT TCAGTCTCTC AGATTGCAAG CATAGATTTA 
CTCAATCTCA GGGATTTGTC CAGTTCTCAA GAAGATAATT TGCCGAATAC ACAGTTACGT 
CATTTGGATA ATGATTATTT GATAAGTAAG CAGGTTAAAG AACATGGTAT TCAAGAACTT 
AGTAGGGAAA GGTCTTTCCA TTCTACTGTT GAAGCCATTT GGGAAGAATG TAAAGAGATT 
GTAAAGACTT CCTCCCAAAA AACCCATCAG ATTGAGGAAC TAGAACAACA AATTGAAAAA 
TTACAGGCAG AAGTAAAAGA CTATACAAAT GAAAACAACA GACTAAAAAT AGAGGAGAGG 
GAGAAAAAGA ATCAAGATGA TTTACTAAAA GAAAAAGATA GTCTTATACA GCAGCTGAAG 
AAAGAAGTAC AAGAAAAAAC CATTAGTCTT GATATTGAAG TACAGCATGT AGTGGAAGGA 
AAGAGAGCAC TTTCAGAACT TACGAAAGGT GTTACTTCCT ATAAGGTGAA AATAAAGGAA 
CTTGAAGCAA TGTTAGAAAC TCAGAAAGAT GAATGTAGCC ATTCAGCCAA GTTAGAACAA 
GAAATTATGG AAAAGGAATC TATCATTTTA AAGCAAGAAA GAAATCTGAA TGAATGTCAA 
GCAAATCTTA AAGATTCTAT CCAAAATGCC AGAGATTTAA GTGAAAGGGA AGTCAAATTG 
AAAGAAGAAA TCATGCAATT AACAAAAAAT TTGCAAGATG CTAAGCATTC ACTTCAATTA 
AAGGAAGAAG AAAAGGAAAC AAACTGGCAA GAAACAGAAA AGTTGAAAGA GGAGCTCTCT 
GCAAGCACTG CTCTTATCCA AAATCTGAAA GCTGATCTCC AGAGGAAGGA AGAAGATTAT 
GCTGAGCTCA AAGAGAAACT GGCCGATGCC AAAAAGCAGA TTGAGCAAGT ACAGAAAGAG 
GTCTCTGTAA TGCGTGATGA GGAGAAATTG TTGAGGATTA AAATTAATGA ACTGGAGAAA 
AAGAAAAACC AGTGTTCTCA GGAAATAGAT ATGAAACAGC GAACCATTCA GCAGCTTAAG 
GAGCAGTTAA ATAATCAAAA GGTGGAAGAA GCTATGCAAC AGTATGAGAG AGTGTGCAAA 
GATCTGAGTG TTAAAGAAAA AATAATCAAA GACATGCAAA TGACCCTGGA AGAACAAGAG 
CAAACTCAGG TAGAACAAGA TCAAGTGCTT GAGGCTAAAT TAGAGGAAAC CGAAAGACTG 
GCCAGAGAAT TGGAAGAATG GAAGGAAAAA TGCAAAGATT TGGAAGCCAA ACACAGTCAA 
AGTTCAAATA AAGAATTTGA GGATAACACA GATGTACTTA ATACAAAACT CACTAAGCTT 
CAAGATGAGT TACAGGAATC TGAACAGAAA CATGAAGCTG AAAGAAAAAA ATGGTTAGAA 
GAAAAAATGA TGCTCATCAC TCAAGCAAAA GAAGCTGAGA GTCTACGAAA CAAAGAGATG 
AAAAAATACG CTGAAGACAG AGAGCATTGT TTGAAGCAAC AAAATGCAAT GGAAATACTT 
AAAGGACAGT TGGCTGAGAA AGAGGGTAAC CTCCAGCAGT GGCGGGAAGA ACGCGATCAA 
CTGGTTGCAG CTTTAGAAAT ACAGCTGAAA GCACTGATCT CCAGTAATGT ACAAAAAGAT 
AATGAAATCA AACAGTTAAA AAAGATCACA TCAGAGACTT CTAAGACAGA AAAACAGACC 
ATGGATACCC AGCCCACACA GACGAGTTCA ACAGATCCTG GCAAGGTTGG ACCTGAACCT 
CAGTCAACAA GTTTTGAAAT TTCCAGAGGT GAAGTAGAGG ATGGATCTGT AGTCCTTGAC 
TCTTGTGAAG TGTCAACAGA AAATAATCAA AGCACTCGAT TTCCAAAACC TGAGTTAGAG 
ATTCAGTTTA CACCTTTACA GCCAAACAGA ATGGCAGTGA AACACCCAGG TTGTACCTCA 
CCAGTGACGG TTAAGATTCC CAGGGCCCGA AAGAGGAAAA GTCATGAAAT GGAAGAGGAC 
TTTGTGAAAT TTGAAAATAA GAAGAATGCT ACACCAAGAA TTAATTTGAA GTCCCCTGTT 
TCTGGGCATA GAAATTCTTC TGTCAAAAAG GAACAAAAGG TTTCCATACG CCCATCATCT 
AAGAAAACTT ACTCTTTACG AAGTCACGCA TCCACAAGTG ATGTAAACGG GCCTGCTAAG 
AAAAAAGAAG GAACGCTGCA GAAATTTGGA GACTTTTTAC AACATTCTCC AATTATTCTT 
CAATCAAAAG CAAAGAAGAT AATTGAAACG ATGAGCTCTT CAAAGTTCTC AAATGTAGAA 
GTAAGTAAAG ATAACGTGTC TCGACCAAAA CGGGCCAAAC GAAAATTATA CACAAATGAA 
ATTTCGTCTC CTATTGATAT ATCTGGCCAA GTGATTTTGA TGGACCATAA AGTGAAAGAA 
AGCGATCATC AGATTCTCAA ACGAAGACTT CGAACCAAAA CAATCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_004020026.4 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004020075.2 Ovis aries kinesin family member 20B (KIF20B), transcript variant X2, mRNA.

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CloneID	OSm73183
Clone ID Related Accession (Same CDS sequence)	XM_004020026.4 , XM_027960158.1 , XM_027960159.1
Accession Version	XM_004020026.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040273.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004020026.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004020075.2
CDS	127..5580
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQTKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_004020026.4
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004020026.4

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_027960158.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027815959.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X1, mRNA.

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CloneID	OSm73183
Clone ID Related Accession (Same CDS sequence)	XM_004020026.4 , XM_027960158.1 , XM_027960159.1
Accession Version	XM_027960158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040273.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027815959.1
CDS	235..5688
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQTKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_027960158.1
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027960158.1

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_027960159.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027815960.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X3, mRNA.

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CloneID	OSm73183
Clone ID Related Accession (Same CDS sequence)	XM_004020026.4 , XM_027960158.1 , XM_027960159.1
Accession Version	XM_027960159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040273.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027815960.1
CDS	230..5683
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQTKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_027960159.1
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027960159.1

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_027960160.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027815961.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X4, mRNA.

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CloneID	OSm73184
Clone ID Related Accession (Same CDS sequence)	XM_027960160.1
Accession Version	XM_027960160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040273.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027815961.1
CDS	235..5637
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQTKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_027960160.1
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027960160.1

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_027960161.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027815962.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X5, mRNA.

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CloneID	OSm73185
Clone ID Related Accession (Same CDS sequence)	XM_027960161.1
Accession Version	XM_027960161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040273.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027815962.1
CDS	235..5568
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFEGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQTKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISVEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQSSYDNVISELHVQKSINQEQKERIIKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQGVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALIQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIKQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_027960161.1
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027960161.1

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCGAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGGT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAACTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGATT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA ACGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTCAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GACTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGTTGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTAAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACAGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTCGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT AAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GGTGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAT CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCAAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_004020026.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004020075.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X4, mRNA.

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CloneID	OSm16083
Clone ID Related Accession (Same CDS sequence)	XM_004020026.3
Accession Version	XM_004020026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004020026.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004020075.1
CDS	156..5609
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFKGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISDEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQLSYDNVISELHVQKSINQEQKERIMKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQDVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALTQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIEQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_004020026.3
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004020026.3

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_004020027.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004020076.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X6, mRNA.

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CloneID	OSm16084
Clone ID Related Accession (Same CDS sequence)	XM_004020027.3
Accession Version	XM_004020027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004020027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004020076.1
CDS	156..5489
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFKGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISDEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQLSYDNVISELHVQKSINQEQKERIMKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQDVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALTQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIEQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEEDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_004020027.3
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004020027.3

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
GACTTTGTGA AATTTGAAAA TAAGAAGAAT GCTACACCAA GAATTAATTT GAAGTCCCCT 
GTTTCTGGGC ATAGAAATTC TTCTGTCAAA AAGGAACAAA AGGTTTCCAT ACGCCCATCA 
TCTAAGAAAA CTTACTCTTT ACGAAGTCAC GCATCCACAA GTGATGTAAA CGGGCCTGCT 
AAGAAAAAAG AAGGAACGCT GCAGAAATTT GGAGACTTTT TACAACATTC TCCAATTATT 
CTTCAATCAA AAGCAAAGAA GATAATTGAA ACGATGAGCT CTTCAAAGTT CTCAAATGTA 
GAAGTAAGTA AAGATAACGT GTCTCGACCA AAACGGGCCA AACGAAAATT ATACACAAAT 
GAAATTTCGT CTCCTATTGA TATATCTGGC CAAGTGATTT TGATGGACCA TAAAGTGAAA 
GAAAGCGATC ATCAGATTCT CAAACGAAGA CTTCGAACCA AAACAATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012102525.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011957915.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X1, mRNA.

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CloneID	OSm43701
Clone ID Related Accession (Same CDS sequence)	XM_012102525.2 , XM_012102526.2 , XM_012102528.2
Accession Version	XM_012102525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012102525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011957915.1
CDS	156..5678
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFKGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISDEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQLSYDNVISELHVQKSINQEQKERIMKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQDVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALTQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIEQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012102525.2
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012102525.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012102526.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011957916.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X2, mRNA.

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CloneID	OSm43701
Clone ID Related Accession (Same CDS sequence)	XM_012102525.2 , XM_012102526.2 , XM_012102528.2
Accession Version	XM_012102526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012102526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011957916.1
CDS	260..5782
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFKGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISDEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQLSYDNVISELHVQKSINQEQKERIMKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQDVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALTQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIEQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012102526.2
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012102526.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012102528.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011957918.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X3, mRNA.

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CloneID	OSm43701
Clone ID Related Accession (Same CDS sequence)	XM_012102525.2 , XM_012102526.2 , XM_012102528.2
Accession Version	XM_012102528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012102528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011957918.1
CDS	255..5777
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFKGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEGHNYVGIEDIIDSLQDDVTGIKKQAEIAHLYIASLADPQEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISDEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQLSYDNVISELHVQKSINQEQKERIMKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQDVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALTQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIEQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012102528.2
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012102528.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGGACAT AATTATGTAG GAATTGAAGA TATTATTGAT 
TCTCTTCAAG ATGATGTCAC TGGTATTAAG AAACAGGCTG AAATTGCTCA CTTATATATT 
GCATCTCTTG CTGACCCCCA GGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF20B ( XM_012102529.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011957919.1 Ovis aries kinesin family member 20B (KIF20B), transcript variant X5, mRNA.

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CloneID	OSm43702
Clone ID Related Accession (Same CDS sequence)	XM_012102529.2
Accession Version	XM_012102529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	kinesin-like protein KIF20B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639031.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012102529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011957919.1
CDS	156..5558
Translation	MESNLNQDGMSRPSYVFSADPLARPSEINFDGIKLDLSREFSLVASGTEANSSESKDYLQVCLRVRPFTQTEKEHESEGCVYILDSQTVVMKEPQCTLGRLSEKGSGQMAQKFSFSKVFGPETTQKEFFKGCILQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENTGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSPDQEKEEVASKSALLRQIKEVVMHNDSYGALYGSLTNSLSIPEFEESMKDCEHSNLNMDINRKFSVWVSFFEIYNECIYDLFVPVSSKFQKRKTLRLSQDVKGYSFIKDLQWIQVSDAKEAYRLLKLGTKHQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPHVIRVSELSLCDLAGSERSMRTQNEGERLRETGNINSSLLTLGKCISVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCSFAYDETLNVLKFSAIAQKVCVPDILNCSQEKSFGSVKSQDESLDINNSDNKILNEKRSTISWESSLEDVVEDEDLVEDLEEAEEKQNVETEFTNEDLDNTLEDDKAFSSHAGKRKLLDLIENLKKKLINERKEKLTLEFKIREEVTQEFTQYLAQREADFKETLLQEREILEENAECRLAIFKDLIGKCDAQEESKNEDHALKDETEEAIGCLEIKFNQVKAELAKTKEELIKTQEELKKRESESEINLNSLVQELEKSNKKIIMQNQRIQELMDKIDQKEDTIMENTCKGSDSVGNSSLLINNKLVCNETTEMSKDSKTKTYSGRKRFNETELQQDEPPAKKGLTHASPAITEDQKKSEETQESISDEENIRVLQENNEELKTRLLTIENELKNEKEEKTELNKQIVSLQQELSSSEKKSLGFSIEVQQIQLSYDNVISELHVQKSINQEQKERIMKLSEEIETARRNITNNVSQIKLMQAKIDELRRLDSVSQIASIDLLNLRDLSSSQEDNLPNTQLRHLDNDYLISKQVKEHGIQELSRERSFHSTVEAIWEECKEIVKTSSQKTHQIEELEQQIEKLQAEVKDYTNENNRLKIEEREKKNQDDLLKEKDSLIQQLKKEVQEKTISLDIEVQHVVEGKRALSELTQDVTSYKVKIKELEAMLETQKDECSHSAKLEQEIMEKESIILKQERNLNECQANLKDSIQNARDLSEREVKLKEEIMQLTKNLQDAKHSLQLKEEEKETNWQETEKLKEELSASTALTQNLKADLQRKEEDYAELKEKLADAKKQIEQVQKEVSVMRDEEKLLRIKINELEKKKNQCSQEIDMKQRTIQQLKEQLNNQKVEEAMQQYERVCKDLSVKEKIIKDMQMTLEEQEQTQVEQDQVLEAKLEETERLARELEEWKEKCKDLEAKHSQSSNKEFEDNTDVLNTKLTKLQDELQESEQKHEAERKKWLEEKMMLITQAKEAESLRNKEMKKYAEDREHCLKQQNAMEILKGQLAEKEGNLQQWREERDQLVAALEIQLKALISSNVQKDNEIEQLKKITSETSKTEKQTMDTQPTQTSSTDPGKVGPEPQSTSFEISRGEVEDGSVVLDSCEVSTENNQSTRFPKPELEIQFTPLQPNRMAVKHPGCTSPVTVKIPRARKRKSHEMEESSVFSSWWKNRNNIKHTPEALCQDFVKFENKKNATPRINLKSPVSGHRNSSVKKEQKVSIRPSSKKTYSLRSHASTSDVNGPAKKKEGTLQKFGDFLQHSPIILQSKAKKIIETMSSSKFSNVEVSKDNVSRPKRAKRKLYTNEISSPIDISGQVILMDHKVKESDHQILKRRLRTKTIK

Target ORF information:

RefSeq Version	XM_012102529.2
Organism	Ovis aries(sheep)
Definition	Ovis aries kinesin family member 20B (KIF20B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012102529.2

ORF Insert Sequence:

ATGGAATCTA ATTTAAATCA AGATGGGATG TCTAGACCAT CTTATGTTTT CAGTGCTGAC 
CCACTTGCAA GACCTTCAGA AATAAATTTT GATGGCATTA AGCTTGACCT CTCTCGTGAA 
TTTTCCTTAG TTGCTTCAGG CACTGAGGCA AACAGTTCCG AATCTAAAGA TTATCTCCAA 
GTTTGTCTTC GAGTAAGACC ATTCACACAG ACAGAAAAAG AACATGAGTC TGAGGGCTGT 
GTGTATATTC TGGATTCACA GACTGTTGTG ATGAAGGAGC CTCAGTGTAC CCTTGGTCGT 
TTAAGTGAGA AAGGCTCTGG GCAGATGGCA CAGAAATTCA GTTTTTCCAA GGTTTTTGGC 
CCAGAAACTA CACAGAAGGA ATTCTTCAAG GGTTGCATTC TGCAGCCAGT TAAAGACCTC 
TTGAAAGGAC AGAGTCGGCT GATTTTTACT TATGGGCTAA CCAATTCTGG AAAAACTTAC 
ACATTTCAAG GCACAGAAGA AAATACCGGC ATTCTTCCTC GAACTTTGAA TGTATTATTT 
GATAGTCTTC AGGAAAGACT GTATACAAAG ATGAACCTTA AACCACATAG GTCCAGAGAA 
TACTTACGAT TATCACCAGA CCAAGAGAAA GAAGAAGTCG CTAGCAAAAG TGCATTACTT 
CGGCAAATTA AGGAGGTTGT TATGCATAAT GACAGTTACG GTGCTCTTTA TGGAAGTCTA 
ACAAACTCTT TGAGTATCCC AGAGTTTGAA GAATCCATGA AAGATTGTGA ACACTCGAAC 
TTGAACATGG ATATTAATAG AAAATTTTCT GTGTGGGTAT CTTTCTTTGA GATTTACAAC 
GAATGTATTT ATGATTTGTT TGTTCCTGTA TCATCTAAGT TCCAGAAGAG AAAGACACTG 
CGCCTTTCCC AAGATGTAAA AGGCTATTCT TTCATAAAAG ATCTACAGTG GATTCAAGTG 
TCTGATGCCA AAGAAGCGTA TAGACTTTTA AAGCTAGGAA CAAAGCACCA GAGTGTTGCT 
TTCACAAAAC TGAACAATGC TTCTAGTAGA AGTCACAGCA TATTTACCAT TAGAATATTA 
CAGATTGAAG ATTCAGAAAT ACCACATGTA ATACGAGTCA GTGAATTGTC TTTATGTGAT 
CTTGCTGGCT CAGAACGAAG CATGAGGACA CAGAATGAAG GTGAAAGGTT AAGAGAGACT 
GGGAACATCA ACTCTTCTTT ATTGACTCTG GGAAAGTGTA TCAGTGTTTT GAAGAACAGC 
GAAAAGTCAA AGTTTCAACA GCATGTGCCT TTCCGGGAAA GTAAATTGAC CCACTATTTT 
CAAAGTTTTT TTAATGGTAA AGGGAAGATA TGTATGATTG TCAACATCAG CCAGTGTTCT 
TTTGCCTATG ATGAGACACT CAATGTGTTG AAGTTCTCAG CCATTGCACA AAAAGTTTGT 
GTTCCAGATA TTTTAAATTG CTCTCAAGAG AAATCATTTG GATCTGTCAA ATCTCAAGAT 
GAATCATTAG ATATTAATAA TTCAGATAAT AAAATACTGA ATGAAAAAAG GTCCACGATT 
TCATGGGAAA GTAGCCTAGA AGATGTGGTG GAAGATGAAG ATTTGGTTGA GGATCTAGAA 
GAAGCTGAAG AAAAGCAGAA TGTGGAAACT GAATTTACTA ATGAAGATCT AGATAATACA 
TTAGAGGATG ATAAGGCTTT CAGTAGCCAT GCGGGGAAGA GAAAACTTTT GGATTTAATA 
GAAAATTTGA AAAAAAAACT AATAAATGAA AGAAAGGAAA AATTAACATT GGAATTTAAA 
ATTCGTGAAG AAGTTACACA GGAATTTACT CAGTATTTGG CCCAACGAGA AGCTGACTTT 
AAGGAAACTC TCCTTCAGGA GCGAGAAATT TTAGAAGAAA ATGCTGAATG TCGTTTGGCT 
ATTTTCAAGG ATTTGATTGG TAAATGTGAT GCTCAAGAAG AATCAAAGAA TGAAGATCAT 
GCCCTGAAGG ATGAAACTGA AGAAGCTATT GGTTGTTTAG AAATAAAGTT TAATCAAGTT 
AAAGCTGAAT TAGCTAAAAC CAAAGAAGAA TTAATCAAAA CCCAAGAAGA GTTAAAAAAG 
AGAGAAAGTG AATCAGAAAT TAATTTAAAT TCATTGGTTC AAGAGCTTGA GAAATCTAAT 
AAGAAAATAA TTATGCAGAA TCAAAGAATT CAAGAGTTAA TGGATAAAAT TGACCAAAAA 
GAGGATACTA TTATGGAAAA CACATGTAAA GGCAGTGACA GTGTTGGTAA TTCTTCTTTA 
CTCATAAACA ATAAATTGGT TTGTAATGAA ACGACTGAAA TGTCTAAGGA CAGCAAAACT 
AAAACGTATT CAGGAAGAAA AAGATTCAAT GAAACTGAAC TTCAACAAGA TGAACCACCA 
GCAAAGAAAG GCCTTACACA TGCTAGTCCA GCTATCACTG AAGACCAAAA GAAAAGTGAA 
GAAACGCAAG AGAGCATTTC AGATGAGGAA AACATTAGAG TTCTACAAGA AAATAATGAA 
GAGCTGAAAA CACGTTTACT CACCATTGAG AATGAACTTA AAAATGAAAA GGAAGAAAAA 
ACGGAATTAA ATAAACAGAT TGTTAGTTTG CAGCAGGAAC TTTCTTCTTC TGAAAAAAAA 
AGTTTAGGCT TTAGTATAGA GGTCCAACAA ATTCAGTTGA GTTATGACAA TGTGATTTCT 
GAATTACATG TGCAGAAAAG TATAAATCAA GAACAGAAGG AAAGGATCAT GAAATTGTCA 
GAGGAGATAG AAACTGCTAG AAGAAACATC ACAAATAATG TTTCCCAAAT AAAATTAATG 
CAGGCAAAAA TAGATGAACT GCGTAGACTT GATTCAGTCT CTCAGATTGC AAGCATAGAT 
TTACTCAATC TCAGGGATTT GTCCAGTTCT CAAGAAGATA ATTTGCCGAA TACACAGTTA 
CGTCATTTGG ATAATGATTA TTTGATAAGT AAGCAGGTTA AAGAACATGG TATTCAAGAA 
CTTAGTAGGG AAAGGTCTTT CCATTCTACT GTTGAAGCCA TTTGGGAAGA ATGTAAAGAG 
ATTGTAAAGA CTTCCTCCCA AAAAACCCAT CAGATTGAGG AACTAGAACA ACAAATTGAA 
AAATTACAGG CAGAAGTAAA AGACTATACA AATGAAAACA ACAGACTAAA AATAGAGGAG 
AGGGAGAAAA AGAATCAAGA TGATTTACTA AAAGAAAAAG ATAGTCTTAT ACAGCAGCTG 
AAGAAAGAAG TACAAGAAAA AACCATTAGT CTTGATATTG AAGTACAGCA TGTAGTGGAA 
GGAAAGAGAG CACTTTCAGA ACTTACGCAA GATGTTACTT CCTATAAGGT GAAAATAAAG 
GAACTTGAAG CAATGTTAGA AACTCAGAAA GATGAATGTA GCCATTCAGC CAAGTTAGAA 
CAAGAAATTA TGGAAAAGGA ATCTATCATT TTAAAGCAAG AAAGAAATCT GAATGAATGT 
CAAGCAAATC TTAAAGATTC TATCCAAAAT GCCAGAGATT TAAGTGAAAG GGAAGTCAAA 
TTGAAAGAAG AAATCATGCA ATTAACAAAA AATTTGCAAG ATGCTAAGCA TTCACTTCAA 
TTAAAGGAAG AAGAAAAGGA AACAAACTGG CAAGAAACAG AAAAGTTGAA AGAGGAGCTC 
TCTGCAAGCA CTGCTCTTAC CCAAAATCTG AAAGCTGATC TCCAGAGGAA GGAAGAAGAT 
TATGCTGAGC TCAAAGAGAA ACTGGCCGAT GCCAAAAAGC AGATTGAGCA AGTACAGAAA 
GAGGTCTCTG TAATGCGTGA TGAGGAGAAA TTGTTGAGGA TTAAAATTAA TGAACTGGAG 
AAAAAGAAAA ACCAGTGTTC TCAGGAAATA GATATGAAAC AGCGAACCAT TCAGCAACTT 
AAGGAGCAGT TAAATAATCA AAAGGTGGAA GAAGCTATGC AACAGTATGA GAGAGTGTGC 
AAAGATCTGA GTGTTAAAGA AAAAATAATC AAAGACATGC AAATGACCCT GGAAGAACAA 
GAGCAAACTC AGGTAGAACA AGATCAAGTG CTTGAGGCTA AATTAGAGGA AACCGAAAGA 
CTGGCCAGAG AATTGGAAGA ATGGAAGGAA AAATGCAAAG ATTTGGAAGC CAAACACAGT 
CAAAGTTCAA ATAAAGAATT TGAGGATAAC ACAGATGTAC TTAATACAAA ACTCACTAAG 
CTTCAAGATG AGTTACAGGA ATCTGAACAG AAACATGAAG CTGAAAGAAA AAAATGGTTA 
GAAGAAAAAA TGATGCTTAT CACTCAAGCA AAAGAAGCTG AGAGTCTACG AAACAAAGAG 
ATGAAAAAAT ACGCTGAAGA CAGAGAGCAT TGTTTGAAGC AACAAAATGC AATGGAAATA 
CTTAAAGGAC AGTTGGCTGA GAAAGAGGGT AACCTCCAGC AGTGGCGGGA AGAACGCGAT 
CAACTGGTTG CAGCTTTAGA AATACAGCTG AAAGCACTGA TCTCCAGTAA TGTACAAAAA 
GATAATGAAA TCGAACAGTT AAAAAAGATC ACATCAGAGA CTTCTAAGAC AGAAAAACAG 
ACCATGGATA CCCAGCCCAC ACAGACGAGT TCAACAGATC CTGGCAAGGT TGGACCTGAA 
CCTCAGTCAA CAAGTTTTGA AATTTCCAGA GGTGAAGTAG AGGATGGATC TGTAGTCCTT 
GACTCTTGTG AAGTGTCAAC AGAAAATAAT CAAAGCACTC GATTTCCAAA ACCTGAGTTA 
GAGATTCAGT TTACACCTTT ACAGCCAAAC AGAATGGCAG TGAAACACCC AGGTTGTACC 
TCACCAGTGA CGGTTAAGAT TCCCAGGGCC CGAAAGAGGA AAAGTCATGA AATGGAAGAG 
AGTAGTGTAT TTTCAAGCTG GTGGAAGAAC AGAAATAATA TTAAACATAC TCCTGAGGCT 
CTCTGTCAGG ACTTTGTGAA ATTTGAAAAT AAGAAGAATG CTACACCAAG AATTAATTTG 
AAGTCCCCTG TTTCTGGGCA TAGAAATTCT TCTGTCAAAA AGGAACAAAA GGTTTCCATA 
CGCCCATCAT CTAAGAAAAC TTACTCTTTA CGAAGTCACG CATCCACAAG TGATGTAAAC 
GGGCCTGCTA AGAAAAAAGA AGGAACGCTG CAGAAATTTG GAGACTTTTT ACAACATTCT 
CCAATTATTC TTCAATCAAA AGCAAAGAAG ATAATTGAAA CGATGAGCTC TTCAAAGTTC 
TCAAATGTAG AAGTAAGTAA AGATAACGTG TCTCGACCAA AACGGGCCAA ACGAAAATTA 
TACACAAATG AAATTTCGTC TCCTATTGAT ATATCTGGCC AAGTGATTTT GATGGACCAT 
AAAGTGAAAG AAAGCGATCA TCAGATTCTC AAACGAAGAC TTCGAACCAA AACAATCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KIF20B
Entrez Gene ID	101122564
Full Name	kinesin family member 20B
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KIF20B cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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