RAPGEF1 gene cDNA ORF clone, Ornithorhynchus anatinus(platypus)

mRNA	Protein	Name
XM_007662218.1	XP_007660408.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_007662219.1	XP_007660409.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_007662220.2	XP_007660410.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_029061996.1	XP_028917829.1	rap guanine nucleotide exchange factor 1 isoform X5
XM_029061994.1	XP_028917827.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_029061995.1	XP_028917828.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_029061999.1	XP_028917832.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_029061997.1	XP_028917830.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_007662214.1	XP_007660404.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_029061993.1	XP_028917826.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_007662214.2	XP_007660404.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_029061998.1	XP_028917831.1	rap guanine nucleotide exchange factor 1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc126889	XM_029061993.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc126890	XM_029061994.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc126891	XM_029061995.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc126892	XM_029061996.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc126893	XM_029061997.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc126894	XM_029061998.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc126895	XM_029061999.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOc01210	XM_007662214.2 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OOc01211	XM_007662218.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OOc01212	XM_007662219.1 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OOc01213	XM_007662220.2 Latest version!	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OOc01210	XM_007662214.1	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

RAPGEF1 ( XM_029061993.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028917826.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc126889
Clone ID Related Accession (Same CDS sequence)	XM_029061993.1
Accession Version	XM_029061993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAGAGGCA ACAGACAGTT TTCTACCAGA GGGCTATGCT 
ACTCCCTTGG ATCTGGAGCA CCAAGCTGTA GAATTTAAAT CCATCAGTGC TGTGATTTCC 
AGGTCTCAGA GGCAGAAGAA TCTAAGCTGG CTGGAAGAGA AGGAGAAGGA AGTCGTCAGT 
GCATTGCGCT ACTTTAAGAC AATTGTGGAC AAAATGGCAA TTGACAAGAA GGTGTTGGAG 
ATGCTTCCTG GCTCTGCCAG CAAGGTGCTA GAGGCTATTC TACCCCTAGT GCAGTCTGAC 
CCTCGGATTC AACAAAGCTC TGCAATCTCT TCCTGCTACA GCCGTGTATA CCAGAGTCTG 
GCCAATCTGA TTCGCTGGTC TGATCAAGTG ATGTTAGAAG GTGTGAACTT AGAAGATAAA 
GAGATGGTGA CAACTGTCAC AGGCGTCATA AAGGCTGTGC TTGATGGAGT GAAGGAGCTG 
GTCAGACTTA CTATAGAAAA ACAGGAGCAT CCATCCCCCA CAAGCCCAGC TAAACTCAAT 
TCACCAGCCT GCAAGTCTGA CGGCCCCTCT GAGCTTCCAC TGACGGATCG AGAAATGGAG 
ATTTTGAACA AGACAACTGG TATGACGCAG TCTACCGAGC AACTCCCTGA TTCCATGGAT 
GAAGAAGTTG CTCCTCCCAA GCCCCCTTTA CCTGGTATTC GAGTGGTGGA TAATAGTCCT 
CCTCCAGCAC TACCACCCAA AAAGCGCCAA TCAGCACCTT CCCCTACGAG AGTGGCAGTG 
GTGGCACCCA TGAGTCGAGC CACCAGTGGC TCTAGTTTAC CTATTGGAAT TAATAGACAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG AGGTTGTCAG GAGGCAGCCA CTCGTATGGT 
GGGGAATCCC CCCGCCTCTC CCCCTGCAAC AGCATGGGCA AGCTCAGCAA ATCCGATGAG 
CAGCTGTCCT CCTTGGACCG GGACAGTGGG CAGTGCTCCC GAAATACAAG CTGTGAAACA 
CTAGATCACT ACGATCCAGA TTATGAATTC CTGCAGGAAG ACCTCTCCAA TGCTGACCAG 
ATACCTCAAC AGGTGCCCTG TAACCTTAGC CCATTGCCAG AATCCTTGGG TGAATCTGTT 
GCTCCATTTT CCGGGAGTCC TTTCAGGCTC TCCCAGAGCA GTTCCCCACA GGCGGAGCAC 
TGCAGTCCTC TACCCTCAAC ACCGTCTTTG GAGACGCCAC CGGCTCTTCC GGAGAAGAAA 
CGGAGGAGCG CCGCCTCTCA GCCCTCGGAC AGTTCCAGCA GTAGGGTCTC CTATGAGAGG 
CACCCTTCCC AATATGACAA CATTTCCGAG GACGACCTGC AGAACCCAGC CAACACACAG 
TCATTACCTT TCACGCCGTT TGCTGCCGTT CTCCCCTTTC AGCAGGGGGC TTCTTCAGCA 
CCAGGGGAAT TTGTTGGGGA TTTTGCTGCT TCAGAGTCTT CAGGTGATCC AGAAAAACCA 
CCTCCTCTCC CAGAGAAGAA GAACAAGCAC ATGTTGGCGT ACATGCAGCT CTTGGAGGAC 
TATTCAGAGC CTCAGCCCTC CATGTTCTAC CAGATGCCCC AGAGCGAGCA CATTTACCAA 
CAGAAGAACA AGTACTTAAT GGAAGTCTAT GGATTCAATG ACTCGTTCAG TAGCGGCGAC 
TCACTGCATG AGTTGGCCCC TCCCCCTGCC CTTCCTCCCA AACAGAGACA GCTGCAGGCC 
TCCTATGCTG CTTCTTCCTT CTCCCCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGCCC 
TTCACTCCTG AGGACACCAG TGCCACTCAA GGCATCAGTA TGTCCATCTC AAACTCCTTT 
CTCAACCGGC ACAGTTCCTT TCCTGTGCAC TCGTATAAGT CTGTGTTTAG GTCCTACTCC 
CAGGATTTCG TGCCTCACAA CCAAGCCTCT GTTCCACCTT TTCTGTCGTC TACCTCCTCT 
TCCACTCCAC ATTTTCCGCC TGTCCACCAC TCTCAGAGCT CTGACCTAGT GGTGGTGCCA 
ACCGCAGCCA ACCCGCCTCC AAGCACTGTG GATGGGCCCA CCTCCTCTTC TCAGGAGAAC 
CGCTTTAATG GGAATGCGTC TGTCTGCCTT CCTTCTGAAC CTCTCACTGA TTCTCTCCAG 
ACGTCTTCGG AATCACCAGT TGCCAAAGAT GGGCACCCTA AAGATTCCTC AACACACACC 
AATGCCACTG GGAAAGAAAG CCGAGAAGGT GGTGAAAGGC AACAAAATTC ACCAGATGCT 
TTGGAATCAG CACAGTCAGA GGAGGAGGTT GATGAGCTCT CCTTGATTGA CCACAGTGAA 
ATTATGTCAA GATTGACACT TAAGCAAGAG GGTGACGATG GGCCAGATGT GCGTGGCGGA 
TCTGGGGATA TCTTGTTGGT CCATGCAACC CAGACTGACA GAAAAGATTT GGTTGTGTAC 
TGTGAAGCCT TTTTGACAAC GTACAGGACG TTCATCACCC CCGAGGAGCT CATCAAAAAG 
CTGCAGTACA GATATGAGAA GTTCTGCCCG TTCCCTGATA CATTTAAGAA GCGTGTCAGC 
AAAAATACTT TCTTTGTGCT GGTGCGAGTG GTGGATGAAT TGTGTCTAGT GGAATTGACA 
GAAGAGATTT TAAAGCTACT GATGGAGCTT GTGTTTCGGT TAGTGTGCAA TGGGGAACTC 
AGCCTTGCCC GAGTGCTGCG GAAAAACATC CTAGATAAAG TGGACCAGAA GAAGATGCTG 
AAATGTGCTA ACTCGGATCA GCCCTTAGCT GCGAGAGGTG TGGCAGCCAG ACCAGGAACC 
CTCCACGATT TTCATAGCCA TGAAATAGCA GAACAGCTAA CGCTGTTAGA CGCCGAACTC 
TTCTATAAAA TAGAGATTCC AGAAGTTTTG CTTTGGGCAA AGGAGCAGAA TGAAGAGAAG 
AGCCCCAACC TGACACAGTT CACTGAGCAC TTTAATAACA TGTCCTACTG GGTCCGATCG 
ATAATAATGC TACAGGAAAA GGCACAGGAT AGGGAGAGGC TGCTCCTGAA ATTCATCAAG 
ATCATGAAGC ACTTGCGAAA GCTGAATAAC TTTAACTCCT ATCTGGCCAT TCTCTCGGCT 
TTGGACTCCG CACCCATCAG AAGGCTAGAG TGGCAGAAGC AGACATCAGA GGGCCTAGCT 
GAGTACTGCA CTTTGATTGA CAGCTCATCC TCCTTCCGGG CCTATCGGGC GGCTCTATCA 
GAAGTAGAAC CTCCCTGTAT CCCATACCTG GGGCTGATCC TGCAGGACCT CACCTTCGTT 
CACCTTGGAA ACCCAGACTA CATTGATGGG AAAGTAAATT TCTCTAAACG CTGGCAACAG 
TTTAACATCT TGGACAGCAT GAGGCGCTTC CAACAAGTAC ATTATGAAAT CCGGAGAAAT 
GACGATATCG TGAACTTCTT CAACGATTTC AGTGATCACC TGGCTGAGGA GGCCCTATGG 
GAACTCTCTC TTAAAATCAA ACCCCGAAAC ATACCACGGC GAAAAACAGA CCGGGAAGAA 
AAAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028917826.1
CDS	174..3902
Translation	MGNANEKQKPLKQNNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLQASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_029061993.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029061993.1

ORF Insert Sequence:

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAGAGGCA ACAGACAGTT TTCTACCAGA GGGCTATGCT 
ACTCCCTTGG ATCTGGAGCA CCAAGCTGTA GAATTTAAAT CCATCAGTGC TGTGATTTCC 
AGGTCTCAGA GGCAGAAGAA TCTAAGCTGG CTGGAAGAGA AGGAGAAGGA AGTCGTCAGT 
GCATTGCGCT ACTTTAAGAC AATTGTGGAC AAAATGGCAA TTGACAAGAA GGTGTTGGAG 
ATGCTTCCTG GCTCTGCCAG CAAGGTGCTA GAGGCTATTC TACCCCTAGT GCAGTCTGAC 
CCTCGGATTC AACAAAGCTC TGCAATCTCT TCCTGCTACA GCCGTGTATA CCAGAGTCTG 
GCCAATCTGA TTCGCTGGTC TGATCAAGTG ATGTTAGAAG GTGTGAACTT AGAAGATAAA 
GAGATGGTGA CAACTGTCAC AGGCGTCATA AAGGCTGTGC TTGATGGAGT GAAGGAGCTG 
GTCAGACTTA CTATAGAAAA ACAGGAGCAT CCATCCCCCA CAAGCCCAGC TAAACTCAAT 
TCACCAGCCT GCAAGTCTGA CGGCCCCTCT GAGCTTCCAC TGACGGATCG AGAAATGGAG 
ATTTTGAACA AGACAACTGG TATGACGCAG TCTACCGAGC AACTCCCTGA TTCCATGGAT 
GAAGAAGTTG CTCCTCCCAA GCCCCCTTTA CCTGGTATTC GAGTGGTGGA TAATAGTCCT 
CCTCCAGCAC TACCACCCAA AAAGCGCCAA TCAGCACCTT CCCCTACGAG AGTGGCAGTG 
GTGGCACCCA TGAGTCGAGC CACCAGTGGC TCTAGTTTAC CTATTGGAAT TAATAGACAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG AGGTTGTCAG GAGGCAGCCA CTCGTATGGT 
GGGGAATCCC CCCGCCTCTC CCCCTGCAAC AGCATGGGCA AGCTCAGCAA ATCCGATGAG 
CAGCTGTCCT CCTTGGACCG GGACAGTGGG CAGTGCTCCC GAAATACAAG CTGTGAAACA 
CTAGATCACT ACGATCCAGA TTATGAATTC CTGCAGGAAG ACCTCTCCAA TGCTGACCAG 
ATACCTCAAC AGGTGCCCTG TAACCTTAGC CCATTGCCAG AATCCTTGGG TGAATCTGTT 
GCTCCATTTT CCGGGAGTCC TTTCAGGCTC TCCCAGAGCA GTTCCCCACA GGCGGAGCAC 
TGCAGTCCTC TACCCTCAAC ACCGTCTTTG GAGACGCCAC CGGCTCTTCC GGAGAAGAAA 
CGGAGGAGCG CCGCCTCTCA GCCCTCGGAC AGTTCCAGCA GTAGGGTCTC CTATGAGAGG 
CACCCTTCCC AATATGACAA CATTTCCGAG GACGACCTGC AGAACCCAGC CAACACACAG 
TCATTACCTT TCACGCCGTT TGCTGCCGTT CTCCCCTTTC AGCAGGGGGC TTCTTCAGCA 
CCAGGGGAAT TTGTTGGGGA TTTTGCTGCT TCAGAGTCTT CAGGTGATCC AGAAAAACCA 
CCTCCTCTCC CAGAGAAGAA GAACAAGCAC ATGTTGGCGT ACATGCAGCT CTTGGAGGAC 
TATTCAGAGC CTCAGCCCTC CATGTTCTAC CAGATGCCCC AGAGCGAGCA CATTTACCAA 
CAGAAGAACA AGTACTTAAT GGAAGTCTAT GGATTCAATG ACTCGTTCAG TAGCGGCGAC 
TCACTGCATG AGTTGGCCCC TCCCCCTGCC CTTCCTCCCA AACAGAGACA GCTGCAGGCC 
TCCTATGCTG CTTCTTCCTT CTCCCCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGCCC 
TTCACTCCTG AGGACACCAG TGCCACTCAA GGCATCAGTA TGTCCATCTC AAACTCCTTT 
CTCAACCGGC ACAGTTCCTT TCCTGTGCAC TCGTATAAGT CTGTGTTTAG GTCCTACTCC 
CAGGATTTCG TGCCTCACAA CCAAGCCTCT GTTCCACCTT TTCTGTCGTC TACCTCCTCT 
TCCACTCCAC ATTTTCCGCC TGTCCACCAC TCTCAGAGCT CTGACCTAGT GGTGGTGCCA 
ACCGCAGCCA ACCCGCCTCC AAGCACTGTG GATGGGCCCA CCTCCTCTTC TCAGGAGAAC 
CGCTTTAATG GGAATGCGTC TGTCTGCCTT CCTTCTGAAC CTCTCACTGA TTCTCTCCAG 
ACGTCTTCGG AATCACCAGT TGCCAAAGAT GGGCACCCTA AAGATTCCTC AACACACACC 
AATGCCACTG GGAAAGAAAG CCGAGAAGGT GGTGAAAGGC AACAAAATTC ACCAGATGCT 
TTGGAATCAG CACAGTCAGA GGAGGAGGTT GATGAGCTCT CCTTGATTGA CCACAGTGAA 
ATTATGTCAA GATTGACACT TAAGCAAGAG GGTGACGATG GGCCAGATGT GCGTGGCGGA 
TCTGGGGATA TCTTGTTGGT CCATGCAACC CAGACTGACA GAAAAGATTT GGTTGTGTAC 
TGTGAAGCCT TTTTGACAAC GTACAGGACG TTCATCACCC CCGAGGAGCT CATCAAAAAG 
CTGCAGTACA GATATGAGAA GTTCTGCCCG TTCCCTGATA CATTTAAGAA GCGTGTCAGC 
AAAAATACTT TCTTTGTGCT GGTGCGAGTG GTGGATGAAT TGTGTCTAGT GGAATTGACA 
GAAGAGATTT TAAAGCTACT GATGGAGCTT GTGTTTCGGT TAGTGTGCAA TGGGGAACTC 
AGCCTTGCCC GAGTGCTGCG GAAAAACATC CTAGATAAAG TGGACCAGAA GAAGATGCTG 
AAATGTGCTA ACTCGGATCA GCCCTTAGCT GCGAGAGGTG TGGCAGCCAG ACCAGGAACC 
CTCCACGATT TTCATAGCCA TGAAATAGCA GAACAGCTAA CGCTGTTAGA CGCCGAACTC 
TTCTATAAAA TAGAGATTCC AGAAGTTTTG CTTTGGGCAA AGGAGCAGAA TGAAGAGAAG 
AGCCCCAACC TGACACAGTT CACTGAGCAC TTTAATAACA TGTCCTACTG GGTCCGATCG 
ATAATAATGC TACAGGAAAA GGCACAGGAT AGGGAGAGGC TGCTCCTGAA ATTCATCAAG 
ATCATGAAGC ACTTGCGAAA GCTGAATAAC TTTAACTCCT ATCTGGCCAT TCTCTCGGCT 
TTGGACTCCG CACCCATCAG AAGGCTAGAG TGGCAGAAGC AGACATCAGA GGGCCTAGCT 
GAGTACTGCA CTTTGATTGA CAGCTCATCC TCCTTCCGGG CCTATCGGGC GGCTCTATCA 
GAAGTAGAAC CTCCCTGTAT CCCATACCTG GGGCTGATCC TGCAGGACCT CACCTTCGTT 
CACCTTGGAA ACCCAGACTA CATTGATGGG AAAGTAAATT TCTCTAAACG CTGGCAACAG 
TTTAACATCT TGGACAGCAT GAGGCGCTTC CAACAAGTAC ATTATGAAAT CCGGAGAAAT 
GACGATATCG TGAACTTCTT CAACGATTTC AGTGATCACC TGGCTGAGGA GGCCCTATGG 
GAACTCTCTC TTAAAATCAA ACCCCGAAAC ATACCACGGC GAAAAACAGA CCGGGAAGAA 
AAAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_029061994.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028917827.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc126890
Clone ID Related Accession (Same CDS sequence)	XM_029061994.1
Accession Version	XM_029061994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAGAGGCA ACAGACAGTT TTCTACCAGA GGGCTATGCT 
ACTCCCTTGG ATCTGGAGCA CCAAGCTGTA GAATTTAAAT CCATCAGTGC TGTGATTTCC 
AGGTCTCAGA GGCAGAAGAA TCTAAGCTGG CTGGAAGAGA AGGAGAAGGA AGTCGTCAGT 
GCATTGCGCT ACTTTAAGAC AATTGTGGAC AAAATGGCAA TTGACAAGAA GGTGTTGGAG 
ATGCTTCCTG GCTCTGCCAG CAAGGTGCTA GAGGCTATTC TACCCCTAGT GCAGTCTGAC 
CCTCGGATTC AACAAAGCTC TGCAATCTCT TCCTGCTACA GCCGTGTATA CCAGAGTCTG 
GCCAATCTGA TTCGCTGGTC TGATCAAGTG ATGTTAGAAG GTGTGAACTT AGAAGATAAA 
GAGATGGTGA CAACTGTCAC AGGCGTCATA AAGGCTGTGC TTGATGGAGT GAAGGAGCTG 
GTCAGACTTA CTATAGAAAA ACAGGAGCAT CCATCCCCCA CAAGCCCAGC TAAACTCAAT 
TCACCAGCCT GCAAGTCTGA CGGCCCCTCT GAGCTTCCAC TGACGGATCG AGAAATGGAG 
ATTTTGAACA AGACAACTGG TATGACGCAG TCTACCGAGC AACTCCCTGA TTCCATGGAT 
GAAGAAGTTG CTCCTCCCAA GCCCCCTTTA CCTGGTATTC GAGTGGTGGA TAATAGTCCT 
CCTCCAGCAC TACCACCCAA AAAGCGCCAA TCAGCACCTT CCCCTACGAG AGTGGCAGTG 
GTGGCACCCA TGAGTCGAGC CACCAGTGGC TCTAGTTTAC CTATTGGAAT TAATAGACAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG AGGTTGTCAG GAGGCAGCCA CTCGTATGGT 
GGGGAATCCC CCCGCCTCTC CCCCTGCAAC AGCATGGGCA AGCTCAGCAA ATCCGATGAG 
CAGCTGTCCT CCTTGGACCG GGACAGTGGG CAGTGCTCCC GAAATACAAG CTGTGAAACA 
CTAGATCACT ACGATCCAGA TTATGAATTC CTGCAGGAAG ACCTCTCCAA TGCTGACCAG 
ATACCTCAAC AGGTGCCCTG TAACCTTAGC CCATTGCCAG AATCCTTGGG TGAATCTGTT 
GCTCCATTTT CCGGGAGTCC TTTCAGGCTC TCCCAGAGCA GTTCCCCACA GGCGGAGCAC 
TGCAGTCCTC TACCCTCAAC ACCGTCTTTG GAGACGCCAC CGGCTCTTCC GGAGAAGAAA 
CGGAGGAGCG CCGCCTCTCA GCCCTCGGAC AGTTCCAGCA GTAGGGTCTC CTATGAGAGG 
CACCCTTCCC AATATGACAA CATTTCCGAG GACGACCTGC AGAACCCAGC CAACACACAG 
TCATTACCTT TCACGCCGTT TGCTGCCGTT CTCCCCTTTC AGCAGGGGGC TTCTTCAGCA 
CCAGGGGAAT TTGTTGGGGA TTTTGCTGCT TCAGAGTCTT CAGGTGATCC AGAAAAACCA 
CCTCCTCTCC CAGAGAAGAA GAACAAGCAC ATGTTGGCGT ACATGCAGCT CTTGGAGGAC 
TATTCAGAGC CTCAGCCCTC CATGTTCTAC CAGATGCCCC AGAGCGAGCA CATTTACCAA 
CAGAAGAACA AGTACTTAAT GGAAGTCTAT GGATTCAATG ACTCGTTCAG TAGCGGCGAC 
TCACTGCATG AGTTGGCCCC TCCCCCTGCC CTTCCTCCCA AACAGAGACA GCTGGCCTCC 
TATGCTGCTT CTTCCTTCTC CCCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGCCCTTC 
ACTCCTGAGG ACACCAGTGC CACTCAAGGC ATCAGTATGT CCATCTCAAA CTCCTTTCTC 
AACCGGCACA GTTCCTTTCC TGTGCACTCG TATAAGTCTG TGTTTAGGTC CTACTCCCAG 
GATTTCGTGC CTCACAACCA AGCCTCTGTT CCACCTTTTC TGTCGTCTAC CTCCTCTTCC 
ACTCCACATT TTCCGCCTGT CCACCACTCT CAGAGCTCTG ACCTAGTGGT GGTGCCAACC 
GCAGCCAACC CGCCTCCAAG CACTGTGGAT GGGCCCACCT CCTCTTCTCA GGAGAACCGC 
TTTAATGGGA ATGCGTCTGT CTGCCTTCCT TCTGAACCTC TCACTGATTC TCTCCAGACG 
TCTTCGGAAT CACCAGTTGC CAAAGATGGG CACCCTAAAG ATTCCTCAAC ACACACCAAT 
GCCACTGGGA AAGAAAGCCG AGAAGGTGGT GAAAGGCAAC AAAATTCACC AGATGCTTTG 
GAATCAGCAC AGTCAGAGGA GGAGGTTGAT GAGCTCTCCT TGATTGACCA CAGTGAAATT 
ATGTCAAGAT TGACACTTAA GCAAGAGGGT GACGATGGGC CAGATGTGCG TGGCGGATCT 
GGGGATATCT TGTTGGTCCA TGCAACCCAG ACTGACAGAA AAGATTTGGT TGTGTACTGT 
GAAGCCTTTT TGACAACGTA CAGGACGTTC ATCACCCCCG AGGAGCTCAT CAAAAAGCTG 
CAGTACAGAT ATGAGAAGTT CTGCCCGTTC CCTGATACAT TTAAGAAGCG TGTCAGCAAA 
AATACTTTCT TTGTGCTGGT GCGAGTGGTG GATGAATTGT GTCTAGTGGA ATTGACAGAA 
GAGATTTTAA AGCTACTGAT GGAGCTTGTG TTTCGGTTAG TGTGCAATGG GGAACTCAGC 
CTTGCCCGAG TGCTGCGGAA AAACATCCTA GATAAAGTGG ACCAGAAGAA GATGCTGAAA 
TGTGCTAACT CGGATCAGCC CTTAGCTGCG AGAGGTGTGG CAGCCAGACC AGGAACCCTC 
CACGATTTTC ATAGCCATGA AATAGCAGAA CAGCTAACGC TGTTAGACGC CGAACTCTTC 
TATAAAATAG AGATTCCAGA AGTTTTGCTT TGGGCAAAGG AGCAGAATGA AGAGAAGAGC 
CCCAACCTGA CACAGTTCAC TGAGCACTTT AATAACATGT CCTACTGGGT CCGATCGATA 
ATAATGCTAC AGGAAAAGGC ACAGGATAGG GAGAGGCTGC TCCTGAAATT CATCAAGATC 
ATGAAGCACT TGCGAAAGCT GAATAACTTT AACTCCTATC TGGCCATTCT CTCGGCTTTG 
GACTCCGCAC CCATCAGAAG GCTAGAGTGG CAGAAGCAGA CATCAGAGGG CCTAGCTGAG 
TACTGCACTT TGATTGACAG CTCATCCTCC TTCCGGGCCT ATCGGGCGGC TCTATCAGAA 
GTAGAACCTC CCTGTATCCC ATACCTGGGG CTGATCCTGC AGGACCTCAC CTTCGTTCAC 
CTTGGAAACC CAGACTACAT TGATGGGAAA GTAAATTTCT CTAAACGCTG GCAACAGTTT 
AACATCTTGG ACAGCATGAG GCGCTTCCAA CAAGTACATT ATGAAATCCG GAGAAATGAC 
GATATCGTGA ACTTCTTCAA CGATTTCAGT GATCACCTGG CTGAGGAGGC CCTATGGGAA 
CTCTCTCTTA AAATCAAACC CCGAAACATA CCACGGCGAA AAACAGACCG GGAAGAAAAA 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028917827.1
CDS	174..3899
Translation	MGNANEKQKPLKQNNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_029061994.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029061994.1

ORF Insert Sequence:

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAGAGGCA ACAGACAGTT TTCTACCAGA GGGCTATGCT 
ACTCCCTTGG ATCTGGAGCA CCAAGCTGTA GAATTTAAAT CCATCAGTGC TGTGATTTCC 
AGGTCTCAGA GGCAGAAGAA TCTAAGCTGG CTGGAAGAGA AGGAGAAGGA AGTCGTCAGT 
GCATTGCGCT ACTTTAAGAC AATTGTGGAC AAAATGGCAA TTGACAAGAA GGTGTTGGAG 
ATGCTTCCTG GCTCTGCCAG CAAGGTGCTA GAGGCTATTC TACCCCTAGT GCAGTCTGAC 
CCTCGGATTC AACAAAGCTC TGCAATCTCT TCCTGCTACA GCCGTGTATA CCAGAGTCTG 
GCCAATCTGA TTCGCTGGTC TGATCAAGTG ATGTTAGAAG GTGTGAACTT AGAAGATAAA 
GAGATGGTGA CAACTGTCAC AGGCGTCATA AAGGCTGTGC TTGATGGAGT GAAGGAGCTG 
GTCAGACTTA CTATAGAAAA ACAGGAGCAT CCATCCCCCA CAAGCCCAGC TAAACTCAAT 
TCACCAGCCT GCAAGTCTGA CGGCCCCTCT GAGCTTCCAC TGACGGATCG AGAAATGGAG 
ATTTTGAACA AGACAACTGG TATGACGCAG TCTACCGAGC AACTCCCTGA TTCCATGGAT 
GAAGAAGTTG CTCCTCCCAA GCCCCCTTTA CCTGGTATTC GAGTGGTGGA TAATAGTCCT 
CCTCCAGCAC TACCACCCAA AAAGCGCCAA TCAGCACCTT CCCCTACGAG AGTGGCAGTG 
GTGGCACCCA TGAGTCGAGC CACCAGTGGC TCTAGTTTAC CTATTGGAAT TAATAGACAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG AGGTTGTCAG GAGGCAGCCA CTCGTATGGT 
GGGGAATCCC CCCGCCTCTC CCCCTGCAAC AGCATGGGCA AGCTCAGCAA ATCCGATGAG 
CAGCTGTCCT CCTTGGACCG GGACAGTGGG CAGTGCTCCC GAAATACAAG CTGTGAAACA 
CTAGATCACT ACGATCCAGA TTATGAATTC CTGCAGGAAG ACCTCTCCAA TGCTGACCAG 
ATACCTCAAC AGGTGCCCTG TAACCTTAGC CCATTGCCAG AATCCTTGGG TGAATCTGTT 
GCTCCATTTT CCGGGAGTCC TTTCAGGCTC TCCCAGAGCA GTTCCCCACA GGCGGAGCAC 
TGCAGTCCTC TACCCTCAAC ACCGTCTTTG GAGACGCCAC CGGCTCTTCC GGAGAAGAAA 
CGGAGGAGCG CCGCCTCTCA GCCCTCGGAC AGTTCCAGCA GTAGGGTCTC CTATGAGAGG 
CACCCTTCCC AATATGACAA CATTTCCGAG GACGACCTGC AGAACCCAGC CAACACACAG 
TCATTACCTT TCACGCCGTT TGCTGCCGTT CTCCCCTTTC AGCAGGGGGC TTCTTCAGCA 
CCAGGGGAAT TTGTTGGGGA TTTTGCTGCT TCAGAGTCTT CAGGTGATCC AGAAAAACCA 
CCTCCTCTCC CAGAGAAGAA GAACAAGCAC ATGTTGGCGT ACATGCAGCT CTTGGAGGAC 
TATTCAGAGC CTCAGCCCTC CATGTTCTAC CAGATGCCCC AGAGCGAGCA CATTTACCAA 
CAGAAGAACA AGTACTTAAT GGAAGTCTAT GGATTCAATG ACTCGTTCAG TAGCGGCGAC 
TCACTGCATG AGTTGGCCCC TCCCCCTGCC CTTCCTCCCA AACAGAGACA GCTGGCCTCC 
TATGCTGCTT CTTCCTTCTC CCCTGTCTCC TACTGTGTCC AGCAAACTAA AGTGCCCTTC 
ACTCCTGAGG ACACCAGTGC CACTCAAGGC ATCAGTATGT CCATCTCAAA CTCCTTTCTC 
AACCGGCACA GTTCCTTTCC TGTGCACTCG TATAAGTCTG TGTTTAGGTC CTACTCCCAG 
GATTTCGTGC CTCACAACCA AGCCTCTGTT CCACCTTTTC TGTCGTCTAC CTCCTCTTCC 
ACTCCACATT TTCCGCCTGT CCACCACTCT CAGAGCTCTG ACCTAGTGGT GGTGCCAACC 
GCAGCCAACC CGCCTCCAAG CACTGTGGAT GGGCCCACCT CCTCTTCTCA GGAGAACCGC 
TTTAATGGGA ATGCGTCTGT CTGCCTTCCT TCTGAACCTC TCACTGATTC TCTCCAGACG 
TCTTCGGAAT CACCAGTTGC CAAAGATGGG CACCCTAAAG ATTCCTCAAC ACACACCAAT 
GCCACTGGGA AAGAAAGCCG AGAAGGTGGT GAAAGGCAAC AAAATTCACC AGATGCTTTG 
GAATCAGCAC AGTCAGAGGA GGAGGTTGAT GAGCTCTCCT TGATTGACCA CAGTGAAATT 
ATGTCAAGAT TGACACTTAA GCAAGAGGGT GACGATGGGC CAGATGTGCG TGGCGGATCT 
GGGGATATCT TGTTGGTCCA TGCAACCCAG ACTGACAGAA AAGATTTGGT TGTGTACTGT 
GAAGCCTTTT TGACAACGTA CAGGACGTTC ATCACCCCCG AGGAGCTCAT CAAAAAGCTG 
CAGTACAGAT ATGAGAAGTT CTGCCCGTTC CCTGATACAT TTAAGAAGCG TGTCAGCAAA 
AATACTTTCT TTGTGCTGGT GCGAGTGGTG GATGAATTGT GTCTAGTGGA ATTGACAGAA 
GAGATTTTAA AGCTACTGAT GGAGCTTGTG TTTCGGTTAG TGTGCAATGG GGAACTCAGC 
CTTGCCCGAG TGCTGCGGAA AAACATCCTA GATAAAGTGG ACCAGAAGAA GATGCTGAAA 
TGTGCTAACT CGGATCAGCC CTTAGCTGCG AGAGGTGTGG CAGCCAGACC AGGAACCCTC 
CACGATTTTC ATAGCCATGA AATAGCAGAA CAGCTAACGC TGTTAGACGC CGAACTCTTC 
TATAAAATAG AGATTCCAGA AGTTTTGCTT TGGGCAAAGG AGCAGAATGA AGAGAAGAGC 
CCCAACCTGA CACAGTTCAC TGAGCACTTT AATAACATGT CCTACTGGGT CCGATCGATA 
ATAATGCTAC AGGAAAAGGC ACAGGATAGG GAGAGGCTGC TCCTGAAATT CATCAAGATC 
ATGAAGCACT TGCGAAAGCT GAATAACTTT AACTCCTATC TGGCCATTCT CTCGGCTTTG 
GACTCCGCAC CCATCAGAAG GCTAGAGTGG CAGAAGCAGA CATCAGAGGG CCTAGCTGAG 
TACTGCACTT TGATTGACAG CTCATCCTCC TTCCGGGCCT ATCGGGCGGC TCTATCAGAA 
GTAGAACCTC CCTGTATCCC ATACCTGGGG CTGATCCTGC AGGACCTCAC CTTCGTTCAC 
CTTGGAAACC CAGACTACAT TGATGGGAAA GTAAATTTCT CTAAACGCTG GCAACAGTTT 
AACATCTTGG ACAGCATGAG GCGCTTCCAA CAAGTACATT ATGAAATCCG GAGAAATGAC 
GATATCGTGA ACTTCTTCAA CGATTTCAGT GATCACCTGG CTGAGGAGGC CCTATGGGAA 
CTCTCTCTTA AAATCAAACC CCGAAACATA CCACGGCGAA AAACAGACCG GGAAGAAAAA 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_029061995.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028917828.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

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CloneID	OOc126891
Clone ID Related Accession (Same CDS sequence)	XM_029061995.1
Accession Version	XM_029061995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACACT CAGCCCCTCA GCACTTATAT TCCCAGCGCT CTCATCTCTC TTCGTTCACT 
ATGAAGCTGA TGGACAAATT TCATTCTCCC AAAATAAAGA GGACACCATC AAAGAAAGGA 
AAACCAGCCG ACCTCACTGT GAAAACCCCA GAAAAGCCAG TCAACAAAGA GGCAACAGAC 
AGTTTTCTAC CAGAGGGCTA TGCTACTCCC TTGGATCTGG AGCACCAAGC TGTAGAATTT 
AAATCCATCA GTGCTGTGAT TTCCAGGTCT CAGAGGCAGA AGAATCTAAG CTGGCTGGAA 
GAGAAGGAGA AGGAAGTCGT CAGTGCATTG CGCTACTTTA AGACAATTGT GGACAAAATG 
GCAATTGACA AGAAGGTGTT GGAGATGCTT CCTGGCTCTG CCAGCAAGGT GCTAGAGGCT 
ATTCTACCCC TAGTGCAGTC TGACCCTCGG ATTCAACAAA GCTCTGCAAT CTCTTCCTGC 
TACAGCCGTG TATACCAGAG TCTGGCCAAT CTGATTCGCT GGTCTGATCA AGTGATGTTA 
GAAGGTGTGA ACTTAGAAGA TAAAGAGATG GTGACAACTG TCACAGGCGT CATAAAGGCT 
GTGCTTGATG GAGTGAAGGA GCTGGTCAGA CTTACTATAG AAAAACAGGA GCATCCATCC 
CCCACAAGCC CAGCTAAACT CAATTCACCA GCCTGCAAGT CTGACGGCCC CTCTGAGCTT 
CCACTGACGG ATCGAGAAAT GGAGATTTTG AACAAGACAA CTGGTATGAC GCAGTCTACC 
GAGCAACTCC CTGATTCCAT GGATGAAGAA GTTGCTCCTC CCAAGCCCCC TTTACCTGGT 
ATTCGAGTGG TGGATAATAG TCCTCCTCCA GCACTACCAC CCAAAAAGCG CCAATCAGCA 
CCTTCCCCTA CGAGAGTGGC AGTGGTGGCA CCCATGAGTC GAGCCACCAG TGGCTCTAGT 
TTACCTATTG GAATTAATAG ACAGGATTTT GATGTTGACT GTTACGCACA GAGGAGGTTG 
TCAGGAGGCA GCCACTCGTA TGGTGGGGAA TCCCCCCGCC TCTCCCCCTG CAACAGCATG 
GGCAAGCTCA GCAAATCCGA TGAGCAGCTG TCCTCCTTGG ACCGGGACAG TGGGCAGTGC 
TCCCGAAATA CAAGCTGTGA AACACTAGAT CACTACGATC CAGATTATGA ATTCCTGCAG 
GAAGACCTCT CCAATGCTGA CCAGATACCT CAACAGGTGC CCTGTAACCT TAGCCCATTG 
CCAGAATCCT TGGGTGAATC TGTTGCTCCA TTTTCCGGGA GTCCTTTCAG GCTCTCCCAG 
AGCAGTTCCC CACAGGCGGA GCACTGCAGT CCTCTACCCT CAACACCGTC TTTGGAGACG 
CCACCGGCTC TTCCGGAGAA GAAACGGAGG AGCGCCGCCT CTCAGCCCTC GGACAGTTCC 
AGCAGTAGGG TCTCCTATGA GAGGCACCCT TCCCAATATG ACAACATTTC CGAGGACGAC 
CTGCAGAACC CAGCCAACAC ACAGTCATTA CCTTTCACGC CGTTTGCTGC CGTTCTCCCC 
TTTCAGCAGG GGGCTTCTTC AGCACCAGGG GAATTTGTTG GGGATTTTGC TGCTTCAGAG 
TCTTCAGGTG ATCCAGAAAA ACCACCTCCT CTCCCAGAGA AGAAGAACAA GCACATGTTG 
GCGTACATGC AGCTCTTGGA GGACTATTCA GAGCCTCAGC CCTCCATGTT CTACCAGATG 
CCCCAGAGCG AGCACATTTA CCAACAGAAG AACAAGTACT TAATGGAAGT CTATGGATTC 
AATGACTCGT TCAGTAGCGG CGACTCACTG CATGAGTTGG CCCCTCCCCC TGCCCTTCCT 
CCCAAACAGA GACAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCCCC TGTCTCCTAC 
TGTGTCCAGC AAACTAAAGT GCCCTTCACT CCTGAGGACA CCAGTGCCAC TCAAGGCATC 
AGTATGTCCA TCTCAAACTC CTTTCTCAAC CGGCACAGTT CCTTTCCTGT GCACTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCAAGC CTCTGTTCCA 
CCTTTTCTGT CGTCTACCTC CTCTTCCACT CCACATTTTC CGCCTGTCCA CCACTCTCAG 
AGCTCTGACC TAGTGGTGGT GCCAACCGCA GCCAACCCGC CTCCAAGCAC TGTGGATGGG 
CCCACCTCCT CTTCTCAGGA GAACCGCTTT AATGGGAATG CGTCTGTCTG CCTTCCTTCT 
GAACCTCTCA CTGATTCTCT CCAGACGTCT TCGGAATCAC CAGTTGCCAA AGATGGGCAC 
CCTAAAGATT CCTCAACACA CACCAATGCC ACTGGGAAAG AAAGCCGAGA AGGTGGTGAA 
AGGCAACAAA ATTCACCAGA TGCTTTGGAA TCAGCACAGT CAGAGGAGGA GGTTGATGAG 
CTCTCCTTGA TTGACCACAG TGAAATTATG TCAAGATTGA CACTTAAGCA AGAGGGTGAC 
GATGGGCCAG ATGTGCGTGG CGGATCTGGG GATATCTTGT TGGTCCATGC AACCCAGACT 
GACAGAAAAG ATTTGGTTGT GTACTGTGAA GCCTTTTTGA CAACGTACAG GACGTTCATC 
ACCCCCGAGG AGCTCATCAA AAAGCTGCAG TACAGATATG AGAAGTTCTG CCCGTTCCCT 
GATACATTTA AGAAGCGTGT CAGCAAAAAT ACTTTCTTTG TGCTGGTGCG AGTGGTGGAT 
GAATTGTGTC TAGTGGAATT GACAGAAGAG ATTTTAAAGC TACTGATGGA GCTTGTGTTT 
CGGTTAGTGT GCAATGGGGA ACTCAGCCTT GCCCGAGTGC TGCGGAAAAA CATCCTAGAT 
AAAGTGGACC AGAAGAAGAT GCTGAAATGT GCTAACTCGG ATCAGCCCTT AGCTGCGAGA 
GGTGTGGCAG CCAGACCAGG AACCCTCCAC GATTTTCATA GCCATGAAAT AGCAGAACAG 
CTAACGCTGT TAGACGCCGA ACTCTTCTAT AAAATAGAGA TTCCAGAAGT TTTGCTTTGG 
GCAAAGGAGC AGAATGAAGA GAAGAGCCCC AACCTGACAC AGTTCACTGA GCACTTTAAT 
AACATGTCCT ACTGGGTCCG ATCGATAATA ATGCTACAGG AAAAGGCACA GGATAGGGAG 
AGGCTGCTCC TGAAATTCAT CAAGATCATG AAGCACTTGC GAAAGCTGAA TAACTTTAAC 
TCCTATCTGG CCATTCTCTC GGCTTTGGAC TCCGCACCCA TCAGAAGGCT AGAGTGGCAG 
AAGCAGACAT CAGAGGGCCT AGCTGAGTAC TGCACTTTGA TTGACAGCTC ATCCTCCTTC 
CGGGCCTATC GGGCGGCTCT ATCAGAAGTA GAACCTCCCT GTATCCCATA CCTGGGGCTG 
ATCCTGCAGG ACCTCACCTT CGTTCACCTT GGAAACCCAG ACTACATTGA TGGGAAAGTA 
AATTTCTCTA AACGCTGGCA ACAGTTTAAC ATCTTGGACA GCATGAGGCG CTTCCAACAA 
GTACATTATG AAATCCGGAG AAATGACGAT ATCGTGAACT TCTTCAACGA TTTCAGTGAT 
CACCTGGCTG AGGAGGCCCT ATGGGAACTC TCTCTTAAAA TCAAACCCCG AAACATACCA 
CGGCGAAAAA CAGACCGGGA AGAAAAAACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028917828.1
CDS	14..3706
Translation	MSHSAPQHLYSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLQASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_029061995.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029061995.1

ORF Insert Sequence:

ATGTCACACT CAGCCCCTCA GCACTTATAT TCCCAGCGCT CTCATCTCTC TTCGTTCACT 
ATGAAGCTGA TGGACAAATT TCATTCTCCC AAAATAAAGA GGACACCATC AAAGAAAGGA 
AAACCAGCCG ACCTCACTGT GAAAACCCCA GAAAAGCCAG TCAACAAAGA GGCAACAGAC 
AGTTTTCTAC CAGAGGGCTA TGCTACTCCC TTGGATCTGG AGCACCAAGC TGTAGAATTT 
AAATCCATCA GTGCTGTGAT TTCCAGGTCT CAGAGGCAGA AGAATCTAAG CTGGCTGGAA 
GAGAAGGAGA AGGAAGTCGT CAGTGCATTG CGCTACTTTA AGACAATTGT GGACAAAATG 
GCAATTGACA AGAAGGTGTT GGAGATGCTT CCTGGCTCTG CCAGCAAGGT GCTAGAGGCT 
ATTCTACCCC TAGTGCAGTC TGACCCTCGG ATTCAACAAA GCTCTGCAAT CTCTTCCTGC 
TACAGCCGTG TATACCAGAG TCTGGCCAAT CTGATTCGCT GGTCTGATCA AGTGATGTTA 
GAAGGTGTGA ACTTAGAAGA TAAAGAGATG GTGACAACTG TCACAGGCGT CATAAAGGCT 
GTGCTTGATG GAGTGAAGGA GCTGGTCAGA CTTACTATAG AAAAACAGGA GCATCCATCC 
CCCACAAGCC CAGCTAAACT CAATTCACCA GCCTGCAAGT CTGACGGCCC CTCTGAGCTT 
CCACTGACGG ATCGAGAAAT GGAGATTTTG AACAAGACAA CTGGTATGAC GCAGTCTACC 
GAGCAACTCC CTGATTCCAT GGATGAAGAA GTTGCTCCTC CCAAGCCCCC TTTACCTGGT 
ATTCGAGTGG TGGATAATAG TCCTCCTCCA GCACTACCAC CCAAAAAGCG CCAATCAGCA 
CCTTCCCCTA CGAGAGTGGC AGTGGTGGCA CCCATGAGTC GAGCCACCAG TGGCTCTAGT 
TTACCTATTG GAATTAATAG ACAGGATTTT GATGTTGACT GTTACGCACA GAGGAGGTTG 
TCAGGAGGCA GCCACTCGTA TGGTGGGGAA TCCCCCCGCC TCTCCCCCTG CAACAGCATG 
GGCAAGCTCA GCAAATCCGA TGAGCAGCTG TCCTCCTTGG ACCGGGACAG TGGGCAGTGC 
TCCCGAAATA CAAGCTGTGA AACACTAGAT CACTACGATC CAGATTATGA ATTCCTGCAG 
GAAGACCTCT CCAATGCTGA CCAGATACCT CAACAGGTGC CCTGTAACCT TAGCCCATTG 
CCAGAATCCT TGGGTGAATC TGTTGCTCCA TTTTCCGGGA GTCCTTTCAG GCTCTCCCAG 
AGCAGTTCCC CACAGGCGGA GCACTGCAGT CCTCTACCCT CAACACCGTC TTTGGAGACG 
CCACCGGCTC TTCCGGAGAA GAAACGGAGG AGCGCCGCCT CTCAGCCCTC GGACAGTTCC 
AGCAGTAGGG TCTCCTATGA GAGGCACCCT TCCCAATATG ACAACATTTC CGAGGACGAC 
CTGCAGAACC CAGCCAACAC ACAGTCATTA CCTTTCACGC CGTTTGCTGC CGTTCTCCCC 
TTTCAGCAGG GGGCTTCTTC AGCACCAGGG GAATTTGTTG GGGATTTTGC TGCTTCAGAG 
TCTTCAGGTG ATCCAGAAAA ACCACCTCCT CTCCCAGAGA AGAAGAACAA GCACATGTTG 
GCGTACATGC AGCTCTTGGA GGACTATTCA GAGCCTCAGC CCTCCATGTT CTACCAGATG 
CCCCAGAGCG AGCACATTTA CCAACAGAAG AACAAGTACT TAATGGAAGT CTATGGATTC 
AATGACTCGT TCAGTAGCGG CGACTCACTG CATGAGTTGG CCCCTCCCCC TGCCCTTCCT 
CCCAAACAGA GACAGCTGCA GGCCTCCTAT GCTGCTTCTT CCTTCTCCCC TGTCTCCTAC 
TGTGTCCAGC AAACTAAAGT GCCCTTCACT CCTGAGGACA CCAGTGCCAC TCAAGGCATC 
AGTATGTCCA TCTCAAACTC CTTTCTCAAC CGGCACAGTT CCTTTCCTGT GCACTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCAAGC CTCTGTTCCA 
CCTTTTCTGT CGTCTACCTC CTCTTCCACT CCACATTTTC CGCCTGTCCA CCACTCTCAG 
AGCTCTGACC TAGTGGTGGT GCCAACCGCA GCCAACCCGC CTCCAAGCAC TGTGGATGGG 
CCCACCTCCT CTTCTCAGGA GAACCGCTTT AATGGGAATG CGTCTGTCTG CCTTCCTTCT 
GAACCTCTCA CTGATTCTCT CCAGACGTCT TCGGAATCAC CAGTTGCCAA AGATGGGCAC 
CCTAAAGATT CCTCAACACA CACCAATGCC ACTGGGAAAG AAAGCCGAGA AGGTGGTGAA 
AGGCAACAAA ATTCACCAGA TGCTTTGGAA TCAGCACAGT CAGAGGAGGA GGTTGATGAG 
CTCTCCTTGA TTGACCACAG TGAAATTATG TCAAGATTGA CACTTAAGCA AGAGGGTGAC 
GATGGGCCAG ATGTGCGTGG CGGATCTGGG GATATCTTGT TGGTCCATGC AACCCAGACT 
GACAGAAAAG ATTTGGTTGT GTACTGTGAA GCCTTTTTGA CAACGTACAG GACGTTCATC 
ACCCCCGAGG AGCTCATCAA AAAGCTGCAG TACAGATATG AGAAGTTCTG CCCGTTCCCT 
GATACATTTA AGAAGCGTGT CAGCAAAAAT ACTTTCTTTG TGCTGGTGCG AGTGGTGGAT 
GAATTGTGTC TAGTGGAATT GACAGAAGAG ATTTTAAAGC TACTGATGGA GCTTGTGTTT 
CGGTTAGTGT GCAATGGGGA ACTCAGCCTT GCCCGAGTGC TGCGGAAAAA CATCCTAGAT 
AAAGTGGACC AGAAGAAGAT GCTGAAATGT GCTAACTCGG ATCAGCCCTT AGCTGCGAGA 
GGTGTGGCAG CCAGACCAGG AACCCTCCAC GATTTTCATA GCCATGAAAT AGCAGAACAG 
CTAACGCTGT TAGACGCCGA ACTCTTCTAT AAAATAGAGA TTCCAGAAGT TTTGCTTTGG 
GCAAAGGAGC AGAATGAAGA GAAGAGCCCC AACCTGACAC AGTTCACTGA GCACTTTAAT 
AACATGTCCT ACTGGGTCCG ATCGATAATA ATGCTACAGG AAAAGGCACA GGATAGGGAG 
AGGCTGCTCC TGAAATTCAT CAAGATCATG AAGCACTTGC GAAAGCTGAA TAACTTTAAC 
TCCTATCTGG CCATTCTCTC GGCTTTGGAC TCCGCACCCA TCAGAAGGCT AGAGTGGCAG 
AAGCAGACAT CAGAGGGCCT AGCTGAGTAC TGCACTTTGA TTGACAGCTC ATCCTCCTTC 
CGGGCCTATC GGGCGGCTCT ATCAGAAGTA GAACCTCCCT GTATCCCATA CCTGGGGCTG 
ATCCTGCAGG ACCTCACCTT CGTTCACCTT GGAAACCCAG ACTACATTGA TGGGAAAGTA 
AATTTCTCTA AACGCTGGCA ACAGTTTAAC ATCTTGGACA GCATGAGGCG CTTCCAACAA 
GTACATTATG AAATCCGGAG AAATGACGAT ATCGTGAACT TCTTCAACGA TTTCAGTGAT 
CACCTGGCTG AGGAGGCCCT ATGGGAACTC TCTCTTAAAA TCAAACCCCG AAACATACCA 
CGGCGAAAAA CAGACCGGGA AGAAAAAACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_029061996.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028917829.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOc126892
Clone ID Related Accession (Same CDS sequence)	XM_029061996.1
Accession Version	XM_029061996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAATT CCCAGCGCTC TCATCTCTCT TCGTTCACTA TGAAGCTGAT GGACAAATTT 
CATTCTCCCA AAATAAAGAG GACACCATCA AAGAAAGGAA AACCAGCCGA CCTCACTGTG 
AAAACCCCAG AAAAGCCAGT CAACAAAGAG GCAACAGACA GTTTTCTACC AGAGGGCTAT 
GCTACTCCCT TGGATCTGGA GCACCAAGCT GTAGAATTTA AATCCATCAG TGCTGTGATT 
TCCAGGTCTC AGAGGCAGAA GAATCTAAGC TGGCTGGAAG AGAAGGAGAA GGAAGTCGTC 
AGTGCATTGC GCTACTTTAA GACAATTGTG GACAAAATGG CAATTGACAA GAAGGTGTTG 
GAGATGCTTC CTGGCTCTGC CAGCAAGGTG CTAGAGGCTA TTCTACCCCT AGTGCAGTCT 
GACCCTCGGA TTCAACAAAG CTCTGCAATC TCTTCCTGCT ACAGCCGTGT ATACCAGAGT 
CTGGCCAATC TGATTCGCTG GTCTGATCAA GTGATGTTAG AAGGTGTGAA CTTAGAAGAT 
AAAGAGATGG TGACAACTGT CACAGGCGTC ATAAAGGCTG TGCTTGATGG AGTGAAGGAG 
CTGGTCAGAC TTACTATAGA AAAACAGGAG CATCCATCCC CCACAAGCCC AGCTAAACTC 
AATTCACCAG CCTGCAAGTC TGACGGCCCC TCTGAGCTTC CACTGACGGA TCGAGAAATG 
GAGATTTTGA ACAAGACAAC TGGTATGACG CAGTCTACCG AGCAACTCCC TGATTCCATG 
GATGAAGAAG TTGCTCCTCC CAAGCCCCCT TTACCTGGTA TTCGAGTGGT GGATAATAGT 
CCTCCTCCAG CACTACCACC CAAAAAGCGC CAATCAGCAC CTTCCCCTAC GAGAGTGGCA 
GTGGTGGCAC CCATGAGTCG AGCCACCAGT GGCTCTAGTT TACCTATTGG AATTAATAGA 
CAGGATTTTG ATGTTGACTG TTACGCACAG AGGAGGTTGT CAGGAGGCAG CCACTCGTAT 
GGTGGGGAAT CCCCCCGCCT CTCCCCCTGC AACAGCATGG GCAAGCTCAG CAAATCCGAT 
GAGCAGCTGT CCTCCTTGGA CCGGGACAGT GGGCAGTGCT CCCGAAATAC AAGCTGTGAA 
ACACTAGATC ACTACGATCC AGATTATGAA TTCCTGCAGG AAGACCTCTC CAATGCTGAC 
CAGATACCTC AACAGGTGCC CTGTAACCTT AGCCCATTGC CAGAATCCTT GGGTGAATCT 
GTTGCTCCAT TTTCCGGGAG TCCTTTCAGG CTCTCCCAGA GCAGTTCCCC ACAGGCGGAG 
CACTGCAGTC CTCTACCCTC AACACCGTCT TTGGAGACGC CACCGGCTCT TCCGGAGAAG 
AAACGGAGGA GCGCCGCCTC TCAGCCCTCG GACAGTTCCA GCAGTAGGGT CTCCTATGAG 
AGGCACCCTT CCCAATATGA CAACATTTCC GAGGACGACC TGCAGAACCC AGCCAACACA 
CAGTCATTAC CTTTCACGCC GTTTGCTGCC GTTCTCCCCT TTCAGCAGGG GGCTTCTTCA 
GCACCAGGGG AATTTGTTGG GGATTTTGCT GCTTCAGAGT CTTCAGGTGA TCCAGAAAAA 
CCACCTCCTC TCCCAGAGAA GAAGAACAAG CACATGTTGG CGTACATGCA GCTCTTGGAG 
GACTATTCAG AGCCTCAGCC CTCCATGTTC TACCAGATGC CCCAGAGCGA GCACATTTAC 
CAACAGAAGA ACAAGTACTT AATGGAAGTC TATGGATTCA ATGACTCGTT CAGTAGCGGC 
GACTCACTGC ATGAGTTGGC CCCTCCCCCT GCCCTTCCTC CCAAACAGAG ACAGCTGCAG 
GCCTCCTATG CTGCTTCTTC CTTCTCCCCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG 
CCCTTCACTC CTGAGGACAC CAGTGCCACT CAAGGCATCA GTATGTCCAT CTCAAACTCC 
TTTCTCAACC GGCACAGTTC CTTTCCTGTG CACTCGTATA AGTCTGTGTT TAGGTCCTAC 
TCCCAGGATT TCGTGCCTCA CAACCAAGCC TCTGTTCCAC CTTTTCTGTC GTCTACCTCC 
TCTTCCACTC CACATTTTCC GCCTGTCCAC CACTCTCAGA GCTCTGACCT AGTGGTGGTG 
CCAACCGCAG CCAACCCGCC TCCAAGCACT GTGGATGGGC CCACCTCCTC TTCTCAGGAG 
AACCGCTTTA ATGGGAATGC GTCTGTCTGC CTTCCTTCTG AACCTCTCAC TGATTCTCTC 
CAGACGTCTT CGGAATCACC AGTTGCCAAA GATGGGCACC CTAAAGATTC CTCAACACAC 
ACCAATGCCA CTGGGAAAGA AAGCCGAGAA GGTGGTGAAA GGCAACAAAA TTCACCAGAT 
GCTTTGGAAT CAGCACAGTC AGAGGAGGAG GTTGATGAGC TCTCCTTGAT TGACCACAGT 
GAAATTATGT CAAGATTGAC ACTTAAGCAA GAGGGTGACG ATGGGCCAGA TGTGCGTGGC 
GGATCTGGGG ATATCTTGTT GGTCCATGCA ACCCAGACTG ACAGAAAAGA TTTGGTTGTG 
TACTGTGAAG CCTTTTTGAC AACGTACAGG ACGTTCATCA CCCCCGAGGA GCTCATCAAA 
AAGCTGCAGT ACAGATATGA GAAGTTCTGC CCGTTCCCTG ATACATTTAA GAAGCGTGTC 
AGCAAAAATA CTTTCTTTGT GCTGGTGCGA GTGGTGGATG AATTGTGTCT AGTGGAATTG 
ACAGAAGAGA TTTTAAAGCT ACTGATGGAG CTTGTGTTTC GGTTAGTGTG CAATGGGGAA 
CTCAGCCTTG CCCGAGTGCT GCGGAAAAAC ATCCTAGATA AAGTGGACCA GAAGAAGATG 
CTGAAATGTG CTAACTCGGA TCAGCCCTTA GCTGCGAGAG GTGTGGCAGC CAGACCAGGA 
ACCCTCCACG ATTTTCATAG CCATGAAATA GCAGAACAGC TAACGCTGTT AGACGCCGAA 
CTCTTCTATA AAATAGAGAT TCCAGAAGTT TTGCTTTGGG CAAAGGAGCA GAATGAAGAG 
AAGAGCCCCA ACCTGACACA GTTCACTGAG CACTTTAATA ACATGTCCTA CTGGGTCCGA 
TCGATAATAA TGCTACAGGA AAAGGCACAG GATAGGGAGA GGCTGCTCCT GAAATTCATC 
AAGATCATGA AGCACTTGCG AAAGCTGAAT AACTTTAACT CCTATCTGGC CATTCTCTCG 
GCTTTGGACT CCGCACCCAT CAGAAGGCTA GAGTGGCAGA AGCAGACATC AGAGGGCCTA 
GCTGAGTACT GCACTTTGAT TGACAGCTCA TCCTCCTTCC GGGCCTATCG GGCGGCTCTA 
TCAGAAGTAG AACCTCCCTG TATCCCATAC CTGGGGCTGA TCCTGCAGGA CCTCACCTTC 
GTTCACCTTG GAAACCCAGA CTACATTGAT GGGAAAGTAA ATTTCTCTAA ACGCTGGCAA 
CAGTTTAACA TCTTGGACAG CATGAGGCGC TTCCAACAAG TACATTATGA AATCCGGAGA 
AATGACGATA TCGTGAACTT CTTCAACGAT TTCAGTGATC ACCTGGCTGA GGAGGCCCTA 
TGGGAACTCT CTCTTAAAAT CAAACCCCGA AACATACCAC GGCGAAAAAC AGACCGGGAA 
GAAAAAACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028917829.1
CDS	45..3716
Translation	MHNSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLQASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_029061996.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029061996.1

ORF Insert Sequence:

ATGCACAATT CCCAGCGCTC TCATCTCTCT TCGTTCACTA TGAAGCTGAT GGACAAATTT 
CATTCTCCCA AAATAAAGAG GACACCATCA AAGAAAGGAA AACCAGCCGA CCTCACTGTG 
AAAACCCCAG AAAAGCCAGT CAACAAAGAG GCAACAGACA GTTTTCTACC AGAGGGCTAT 
GCTACTCCCT TGGATCTGGA GCACCAAGCT GTAGAATTTA AATCCATCAG TGCTGTGATT 
TCCAGGTCTC AGAGGCAGAA GAATCTAAGC TGGCTGGAAG AGAAGGAGAA GGAAGTCGTC 
AGTGCATTGC GCTACTTTAA GACAATTGTG GACAAAATGG CAATTGACAA GAAGGTGTTG 
GAGATGCTTC CTGGCTCTGC CAGCAAGGTG CTAGAGGCTA TTCTACCCCT AGTGCAGTCT 
GACCCTCGGA TTCAACAAAG CTCTGCAATC TCTTCCTGCT ACAGCCGTGT ATACCAGAGT 
CTGGCCAATC TGATTCGCTG GTCTGATCAA GTGATGTTAG AAGGTGTGAA CTTAGAAGAT 
AAAGAGATGG TGACAACTGT CACAGGCGTC ATAAAGGCTG TGCTTGATGG AGTGAAGGAG 
CTGGTCAGAC TTACTATAGA AAAACAGGAG CATCCATCCC CCACAAGCCC AGCTAAACTC 
AATTCACCAG CCTGCAAGTC TGACGGCCCC TCTGAGCTTC CACTGACGGA TCGAGAAATG 
GAGATTTTGA ACAAGACAAC TGGTATGACG CAGTCTACCG AGCAACTCCC TGATTCCATG 
GATGAAGAAG TTGCTCCTCC CAAGCCCCCT TTACCTGGTA TTCGAGTGGT GGATAATAGT 
CCTCCTCCAG CACTACCACC CAAAAAGCGC CAATCAGCAC CTTCCCCTAC GAGAGTGGCA 
GTGGTGGCAC CCATGAGTCG AGCCACCAGT GGCTCTAGTT TACCTATTGG AATTAATAGA 
CAGGATTTTG ATGTTGACTG TTACGCACAG AGGAGGTTGT CAGGAGGCAG CCACTCGTAT 
GGTGGGGAAT CCCCCCGCCT CTCCCCCTGC AACAGCATGG GCAAGCTCAG CAAATCCGAT 
GAGCAGCTGT CCTCCTTGGA CCGGGACAGT GGGCAGTGCT CCCGAAATAC AAGCTGTGAA 
ACACTAGATC ACTACGATCC AGATTATGAA TTCCTGCAGG AAGACCTCTC CAATGCTGAC 
CAGATACCTC AACAGGTGCC CTGTAACCTT AGCCCATTGC CAGAATCCTT GGGTGAATCT 
GTTGCTCCAT TTTCCGGGAG TCCTTTCAGG CTCTCCCAGA GCAGTTCCCC ACAGGCGGAG 
CACTGCAGTC CTCTACCCTC AACACCGTCT TTGGAGACGC CACCGGCTCT TCCGGAGAAG 
AAACGGAGGA GCGCCGCCTC TCAGCCCTCG GACAGTTCCA GCAGTAGGGT CTCCTATGAG 
AGGCACCCTT CCCAATATGA CAACATTTCC GAGGACGACC TGCAGAACCC AGCCAACACA 
CAGTCATTAC CTTTCACGCC GTTTGCTGCC GTTCTCCCCT TTCAGCAGGG GGCTTCTTCA 
GCACCAGGGG AATTTGTTGG GGATTTTGCT GCTTCAGAGT CTTCAGGTGA TCCAGAAAAA 
CCACCTCCTC TCCCAGAGAA GAAGAACAAG CACATGTTGG CGTACATGCA GCTCTTGGAG 
GACTATTCAG AGCCTCAGCC CTCCATGTTC TACCAGATGC CCCAGAGCGA GCACATTTAC 
CAACAGAAGA ACAAGTACTT AATGGAAGTC TATGGATTCA ATGACTCGTT CAGTAGCGGC 
GACTCACTGC ATGAGTTGGC CCCTCCCCCT GCCCTTCCTC CCAAACAGAG ACAGCTGCAG 
GCCTCCTATG CTGCTTCTTC CTTCTCCCCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG 
CCCTTCACTC CTGAGGACAC CAGTGCCACT CAAGGCATCA GTATGTCCAT CTCAAACTCC 
TTTCTCAACC GGCACAGTTC CTTTCCTGTG CACTCGTATA AGTCTGTGTT TAGGTCCTAC 
TCCCAGGATT TCGTGCCTCA CAACCAAGCC TCTGTTCCAC CTTTTCTGTC GTCTACCTCC 
TCTTCCACTC CACATTTTCC GCCTGTCCAC CACTCTCAGA GCTCTGACCT AGTGGTGGTG 
CCAACCGCAG CCAACCCGCC TCCAAGCACT GTGGATGGGC CCACCTCCTC TTCTCAGGAG 
AACCGCTTTA ATGGGAATGC GTCTGTCTGC CTTCCTTCTG AACCTCTCAC TGATTCTCTC 
CAGACGTCTT CGGAATCACC AGTTGCCAAA GATGGGCACC CTAAAGATTC CTCAACACAC 
ACCAATGCCA CTGGGAAAGA AAGCCGAGAA GGTGGTGAAA GGCAACAAAA TTCACCAGAT 
GCTTTGGAAT CAGCACAGTC AGAGGAGGAG GTTGATGAGC TCTCCTTGAT TGACCACAGT 
GAAATTATGT CAAGATTGAC ACTTAAGCAA GAGGGTGACG ATGGGCCAGA TGTGCGTGGC 
GGATCTGGGG ATATCTTGTT GGTCCATGCA ACCCAGACTG ACAGAAAAGA TTTGGTTGTG 
TACTGTGAAG CCTTTTTGAC AACGTACAGG ACGTTCATCA CCCCCGAGGA GCTCATCAAA 
AAGCTGCAGT ACAGATATGA GAAGTTCTGC CCGTTCCCTG ATACATTTAA GAAGCGTGTC 
AGCAAAAATA CTTTCTTTGT GCTGGTGCGA GTGGTGGATG AATTGTGTCT AGTGGAATTG 
ACAGAAGAGA TTTTAAAGCT ACTGATGGAG CTTGTGTTTC GGTTAGTGTG CAATGGGGAA 
CTCAGCCTTG CCCGAGTGCT GCGGAAAAAC ATCCTAGATA AAGTGGACCA GAAGAAGATG 
CTGAAATGTG CTAACTCGGA TCAGCCCTTA GCTGCGAGAG GTGTGGCAGC CAGACCAGGA 
ACCCTCCACG ATTTTCATAG CCATGAAATA GCAGAACAGC TAACGCTGTT AGACGCCGAA 
CTCTTCTATA AAATAGAGAT TCCAGAAGTT TTGCTTTGGG CAAAGGAGCA GAATGAAGAG 
AAGAGCCCCA ACCTGACACA GTTCACTGAG CACTTTAATA ACATGTCCTA CTGGGTCCGA 
TCGATAATAA TGCTACAGGA AAAGGCACAG GATAGGGAGA GGCTGCTCCT GAAATTCATC 
AAGATCATGA AGCACTTGCG AAAGCTGAAT AACTTTAACT CCTATCTGGC CATTCTCTCG 
GCTTTGGACT CCGCACCCAT CAGAAGGCTA GAGTGGCAGA AGCAGACATC AGAGGGCCTA 
GCTGAGTACT GCACTTTGAT TGACAGCTCA TCCTCCTTCC GGGCCTATCG GGCGGCTCTA 
TCAGAAGTAG AACCTCCCTG TATCCCATAC CTGGGGCTGA TCCTGCAGGA CCTCACCTTC 
GTTCACCTTG GAAACCCAGA CTACATTGAT GGGAAAGTAA ATTTCTCTAA ACGCTGGCAA 
CAGTTTAACA TCTTGGACAG CATGAGGCGC TTCCAACAAG TACATTATGA AATCCGGAGA 
AATGACGATA TCGTGAACTT CTTCAACGAT TTCAGTGATC ACCTGGCTGA GGAGGCCCTA 
TGGGAACTCT CTCTTAAAAT CAAACCCCGA AACATACCAC GGCGAAAAAC AGACCGGGAA 
GAAAAAACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_029061997.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028917830.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OOc126893
Clone ID Related Accession (Same CDS sequence)	XM_029061997.1
Accession Version	XM_029061997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAAATCTA AGCTGGCTGG AAGAGAAGGA GAAGGAAGTC 
GTCAGTGCAT TGCGCTACTT TAAGACAATT GTGGACAAAA TGGCAATTGA CAAGAAGGTG 
TTGGAGATGC TTCCTGGCTC TGCCAGCAAG GTGCTAGAGG CTATTCTACC CCTAGTGCAG 
TCTGACCCTC GGATTCAACA AAGCTCTGCA ATCTCTTCCT GCTACAGCCG TGTATACCAG 
AGTCTGGCCA ATCTGATTCG CTGGTCTGAT CAAGTGATGT TAGAAGGTGT GAACTTAGAA 
GATAAAGAGA TGGTGACAAC TGTCACAGGC GTCATAAAGG CTGTGCTTGA TGGAGTGAAG 
GAGCTGGTCA GACTTACTAT AGAAAAACAG GAGCATCCAT CCCCCACAAG CCCAGCTAAA 
CTCAATTCAC CAGCCTGCAA GTCTGACGGC CCCTCTGAGC TTCCACTGAC GGATCGAGAA 
ATGGAGATTT TGAACAAGAC AACTGGTATG ACGCAGTCTA CCGAGCAACT CCCTGATTCC 
ATGGATGAAG AAGTTGCTCC TCCCAAGCCC CCTTTACCTG GTATTCGAGT GGTGGATAAT 
AGTCCTCCTC CAGCACTACC ACCCAAAAAG CGCCAATCAG CACCTTCCCC TACGAGAGTG 
GCAGTGGTGG CACCCATGAG TCGAGCCACC AGTGGCTCTA GTTTACCTAT TGGAATTAAT 
AGACAGGATT TTGATGTTGA CTGTTACGCA CAGAGGAGGT TGTCAGGAGG CAGCCACTCG 
TATGGTGGGG AATCCCCCCG CCTCTCCCCC TGCAACAGCA TGGGCAAGCT CAGCAAATCC 
GATGAGCAGC TGTCCTCCTT GGACCGGGAC AGTGGGCAGT GCTCCCGAAA TACAAGCTGT 
GAAACACTAG ATCACTACGA TCCAGATTAT GAATTCCTGC AGGAAGACCT CTCCAATGCT 
GACCAGATAC CTCAACAGGT GCCCTGTAAC CTTAGCCCAT TGCCAGAATC CTTGGGTGAA 
TCTGTTGCTC CATTTTCCGG GAGTCCTTTC AGGCTCTCCC AGAGCAGTTC CCCACAGGCG 
GAGCACTGCA GTCCTCTACC CTCAACACCG TCTTTGGAGA CGCCACCGGC TCTTCCGGAG 
AAGAAACGGA GGAGCGCCGC CTCTCAGCCC TCGGACAGTT CCAGCAGTAG GGTCTCCTAT 
GAGAGGCACC CTTCCCAATA TGACAACATT TCCGAGGACG ACCTGCAGAA CCCAGCCAAC 
ACACAGTCAT TACCTTTCAC GCCGTTTGCT GCCGTTCTCC CCTTTCAGCA GGGGGCTTCT 
TCAGCACCAG GGGAATTTGT TGGGGATTTT GCTGCTTCAG AGTCTTCAGG TGATCCAGAA 
AAACCACCTC CTCTCCCAGA GAAGAAGAAC AAGCACATGT TGGCGTACAT GCAGCTCTTG 
GAGGACTATT CAGAGCCTCA GCCCTCCATG TTCTACCAGA TGCCCCAGAG CGAGCACATT 
TACCAACAGA AGAACAAGTA CTTAATGGAA GTCTATGGAT TCAATGACTC GTTCAGTAGC 
GGCGACTCAC TGCATGAGTT GGCCCCTCCC CCTGCCCTTC CTCCCAAACA GAGACAGCTG 
CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC CCTGTCTCCT ACTGTGTCCA GCAAACTAAA 
GTGCCCTTCA CTCCTGAGGA CACCAGTGCC ACTCAAGGCA TCAGTATGTC CATCTCAAAC 
TCCTTTCTCA ACCGGCACAG TTCCTTTCCT GTGCACTCGT ATAAGTCTGT GTTTAGGTCC 
TACTCCCAGG ATTTCGTGCC TCACAACCAA GCCTCTGTTC CACCTTTTCT GTCGTCTACC 
TCCTCTTCCA CTCCACATTT TCCGCCTGTC CACCACTCTC AGAGCTCTGA CCTAGTGGTG 
GTGCCAACCG CAGCCAACCC GCCTCCAAGC ACTGTGGATG GGCCCACCTC CTCTTCTCAG 
GAGAACCGCT TTAATGGGAA TGCGTCTGTC TGCCTTCCTT CTGAACCTCT CACTGATTCT 
CTCCAGACGT CTTCGGAATC ACCAGTTGCC AAAGATGGGC ACCCTAAAGA TTCCTCAACA 
CACACCAATG CCACTGGGAA AGAAAGCCGA GAAGGTGGTG AAAGGCAACA AAATTCACCA 
GATGCTTTGG AATCAGCACA GTCAGAGGAG GAGGTTGATG AGCTCTCCTT GATTGACCAC 
AGTGAAATTA TGTCAAGATT GACACTTAAG CAAGAGGGTG ACGATGGGCC AGATGTGCGT 
GGCGGATCTG GGGATATCTT GTTGGTCCAT GCAACCCAGA CTGACAGAAA AGATTTGGTT 
GTGTACTGTG AAGCCTTTTT GACAACGTAC AGGACGTTCA TCACCCCCGA GGAGCTCATC 
AAAAAGCTGC AGTACAGATA TGAGAAGTTC TGCCCGTTCC CTGATACATT TAAGAAGCGT 
GTCAGCAAAA ATACTTTCTT TGTGCTGGTG CGAGTGGTGG ATGAATTGTG TCTAGTGGAA 
TTGACAGAAG AGATTTTAAA GCTACTGATG GAGCTTGTGT TTCGGTTAGT GTGCAATGGG 
GAACTCAGCC TTGCCCGAGT GCTGCGGAAA AACATCCTAG ATAAAGTGGA CCAGAAGAAG 
ATGCTGAAAT GTGCTAACTC GGATCAGCCC TTAGCTGCGA GAGGTGTGGC AGCCAGACCA 
GGAACCCTCC ACGATTTTCA TAGCCATGAA ATAGCAGAAC AGCTAACGCT GTTAGACGCC 
GAACTCTTCT ATAAAATAGA GATTCCAGAA GTTTTGCTTT GGGCAAAGGA GCAGAATGAA 
GAGAAGAGCC CCAACCTGAC ACAGTTCACT GAGCACTTTA ATAACATGTC CTACTGGGTC 
CGATCGATAA TAATGCTACA GGAAAAGGCA CAGGATAGGG AGAGGCTGCT CCTGAAATTC 
ATCAAGATCA TGAAGCACTT GCGAAAGCTG AATAACTTTA ACTCCTATCT GGCCATTCTC 
TCGGCTTTGG ACTCCGCACC CATCAGAAGG CTAGAGTGGC AGAAGCAGAC ATCAGAGGGC 
CTAGCTGAGT ACTGCACTTT GATTGACAGC TCATCCTCCT TCCGGGCCTA TCGGGCGGCT 
CTATCAGAAG TAGAACCTCC CTGTATCCCA TACCTGGGGC TGATCCTGCA GGACCTCACC 
TTCGTTCACC TTGGAAACCC AGACTACATT GATGGGAAAG TAAATTTCTC TAAACGCTGG 
CAACAGTTTA ACATCTTGGA CAGCATGAGG CGCTTCCAAC AAGTACATTA TGAAATCCGG 
AGAAATGACG ATATCGTGAA CTTCTTCAAC GATTTCAGTG ATCACCTGGC TGAGGAGGCC 
CTATGGGAAC TCTCTCTTAA AATCAAACCC CGAAACATAC CACGGCGAAA AACAGACCGG 
GAAGAAAAAA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028917830.1
CDS	172..3786
Translation	MGNANEKQKPLKQNNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLQASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_029061997.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029061997.1

ORF Insert Sequence:

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAAATCTA AGCTGGCTGG AAGAGAAGGA GAAGGAAGTC 
GTCAGTGCAT TGCGCTACTT TAAGACAATT GTGGACAAAA TGGCAATTGA CAAGAAGGTG 
TTGGAGATGC TTCCTGGCTC TGCCAGCAAG GTGCTAGAGG CTATTCTACC CCTAGTGCAG 
TCTGACCCTC GGATTCAACA AAGCTCTGCA ATCTCTTCCT GCTACAGCCG TGTATACCAG 
AGTCTGGCCA ATCTGATTCG CTGGTCTGAT CAAGTGATGT TAGAAGGTGT GAACTTAGAA 
GATAAAGAGA TGGTGACAAC TGTCACAGGC GTCATAAAGG CTGTGCTTGA TGGAGTGAAG 
GAGCTGGTCA GACTTACTAT AGAAAAACAG GAGCATCCAT CCCCCACAAG CCCAGCTAAA 
CTCAATTCAC CAGCCTGCAA GTCTGACGGC CCCTCTGAGC TTCCACTGAC GGATCGAGAA 
ATGGAGATTT TGAACAAGAC AACTGGTATG ACGCAGTCTA CCGAGCAACT CCCTGATTCC 
ATGGATGAAG AAGTTGCTCC TCCCAAGCCC CCTTTACCTG GTATTCGAGT GGTGGATAAT 
AGTCCTCCTC CAGCACTACC ACCCAAAAAG CGCCAATCAG CACCTTCCCC TACGAGAGTG 
GCAGTGGTGG CACCCATGAG TCGAGCCACC AGTGGCTCTA GTTTACCTAT TGGAATTAAT 
AGACAGGATT TTGATGTTGA CTGTTACGCA CAGAGGAGGT TGTCAGGAGG CAGCCACTCG 
TATGGTGGGG AATCCCCCCG CCTCTCCCCC TGCAACAGCA TGGGCAAGCT CAGCAAATCC 
GATGAGCAGC TGTCCTCCTT GGACCGGGAC AGTGGGCAGT GCTCCCGAAA TACAAGCTGT 
GAAACACTAG ATCACTACGA TCCAGATTAT GAATTCCTGC AGGAAGACCT CTCCAATGCT 
GACCAGATAC CTCAACAGGT GCCCTGTAAC CTTAGCCCAT TGCCAGAATC CTTGGGTGAA 
TCTGTTGCTC CATTTTCCGG GAGTCCTTTC AGGCTCTCCC AGAGCAGTTC CCCACAGGCG 
GAGCACTGCA GTCCTCTACC CTCAACACCG TCTTTGGAGA CGCCACCGGC TCTTCCGGAG 
AAGAAACGGA GGAGCGCCGC CTCTCAGCCC TCGGACAGTT CCAGCAGTAG GGTCTCCTAT 
GAGAGGCACC CTTCCCAATA TGACAACATT TCCGAGGACG ACCTGCAGAA CCCAGCCAAC 
ACACAGTCAT TACCTTTCAC GCCGTTTGCT GCCGTTCTCC CCTTTCAGCA GGGGGCTTCT 
TCAGCACCAG GGGAATTTGT TGGGGATTTT GCTGCTTCAG AGTCTTCAGG TGATCCAGAA 
AAACCACCTC CTCTCCCAGA GAAGAAGAAC AAGCACATGT TGGCGTACAT GCAGCTCTTG 
GAGGACTATT CAGAGCCTCA GCCCTCCATG TTCTACCAGA TGCCCCAGAG CGAGCACATT 
TACCAACAGA AGAACAAGTA CTTAATGGAA GTCTATGGAT TCAATGACTC GTTCAGTAGC 
GGCGACTCAC TGCATGAGTT GGCCCCTCCC CCTGCCCTTC CTCCCAAACA GAGACAGCTG 
CAGGCCTCCT ATGCTGCTTC TTCCTTCTCC CCTGTCTCCT ACTGTGTCCA GCAAACTAAA 
GTGCCCTTCA CTCCTGAGGA CACCAGTGCC ACTCAAGGCA TCAGTATGTC CATCTCAAAC 
TCCTTTCTCA ACCGGCACAG TTCCTTTCCT GTGCACTCGT ATAAGTCTGT GTTTAGGTCC 
TACTCCCAGG ATTTCGTGCC TCACAACCAA GCCTCTGTTC CACCTTTTCT GTCGTCTACC 
TCCTCTTCCA CTCCACATTT TCCGCCTGTC CACCACTCTC AGAGCTCTGA CCTAGTGGTG 
GTGCCAACCG CAGCCAACCC GCCTCCAAGC ACTGTGGATG GGCCCACCTC CTCTTCTCAG 
GAGAACCGCT TTAATGGGAA TGCGTCTGTC TGCCTTCCTT CTGAACCTCT CACTGATTCT 
CTCCAGACGT CTTCGGAATC ACCAGTTGCC AAAGATGGGC ACCCTAAAGA TTCCTCAACA 
CACACCAATG CCACTGGGAA AGAAAGCCGA GAAGGTGGTG AAAGGCAACA AAATTCACCA 
GATGCTTTGG AATCAGCACA GTCAGAGGAG GAGGTTGATG AGCTCTCCTT GATTGACCAC 
AGTGAAATTA TGTCAAGATT GACACTTAAG CAAGAGGGTG ACGATGGGCC AGATGTGCGT 
GGCGGATCTG GGGATATCTT GTTGGTCCAT GCAACCCAGA CTGACAGAAA AGATTTGGTT 
GTGTACTGTG AAGCCTTTTT GACAACGTAC AGGACGTTCA TCACCCCCGA GGAGCTCATC 
AAAAAGCTGC AGTACAGATA TGAGAAGTTC TGCCCGTTCC CTGATACATT TAAGAAGCGT 
GTCAGCAAAA ATACTTTCTT TGTGCTGGTG CGAGTGGTGG ATGAATTGTG TCTAGTGGAA 
TTGACAGAAG AGATTTTAAA GCTACTGATG GAGCTTGTGT TTCGGTTAGT GTGCAATGGG 
GAACTCAGCC TTGCCCGAGT GCTGCGGAAA AACATCCTAG ATAAAGTGGA CCAGAAGAAG 
ATGCTGAAAT GTGCTAACTC GGATCAGCCC TTAGCTGCGA GAGGTGTGGC AGCCAGACCA 
GGAACCCTCC ACGATTTTCA TAGCCATGAA ATAGCAGAAC AGCTAACGCT GTTAGACGCC 
GAACTCTTCT ATAAAATAGA GATTCCAGAA GTTTTGCTTT GGGCAAAGGA GCAGAATGAA 
GAGAAGAGCC CCAACCTGAC ACAGTTCACT GAGCACTTTA ATAACATGTC CTACTGGGTC 
CGATCGATAA TAATGCTACA GGAAAAGGCA CAGGATAGGG AGAGGCTGCT CCTGAAATTC 
ATCAAGATCA TGAAGCACTT GCGAAAGCTG AATAACTTTA ACTCCTATCT GGCCATTCTC 
TCGGCTTTGG ACTCCGCACC CATCAGAAGG CTAGAGTGGC AGAAGCAGAC ATCAGAGGGC 
CTAGCTGAGT ACTGCACTTT GATTGACAGC TCATCCTCCT TCCGGGCCTA TCGGGCGGCT 
CTATCAGAAG TAGAACCTCC CTGTATCCCA TACCTGGGGC TGATCCTGCA GGACCTCACC 
TTCGTTCACC TTGGAAACCC AGACTACATT GATGGGAAAG TAAATTTCTC TAAACGCTGG 
CAACAGTTTA ACATCTTGGA CAGCATGAGG CGCTTCCAAC AAGTACATTA TGAAATCCGG 
AGAAATGACG ATATCGTGAA CTTCTTCAAC GATTTCAGTG ATCACCTGGC TGAGGAGGCC 
CTATGGGAAC TCTCTCTTAA AATCAAACCC CGAAACATAC CACGGCGAAA AACAGACCGG 
GAAGAAAAAA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_029061998.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028917831.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OOc126894
Clone ID Related Accession (Same CDS sequence)	XM_029061998.1
Accession Version	XM_029061998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAAATCTA AGCTGGCTGG AAGAGAAGGA GAAGGAAGTC 
GTCAGTGCAT TGCGCTACTT TAAGACAATT GTGGACAAAA TGGCAATTGA CAAGAAGGTG 
TTGGAGATGC TTCCTGGCTC TGCCAGCAAG GTGCTAGAGG CTATTCTACC CCTAGTGCAG 
TCTGACCCTC GGATTCAACA AAGCTCTGCA ATCTCTTCCT GCTACAGCCG TGTATACCAG 
AGTCTGGCCA ATCTGATTCG CTGGTCTGAT CAAGTGATGT TAGAAGGTGT GAACTTAGAA 
GATAAAGAGA TGGTGACAAC TGTCACAGGC GTCATAAAGG CTGTGCTTGA TGGAGTGAAG 
GAGCTGGTCA GACTTACTAT AGAAAAACAG GAGCATCCAT CCCCCACAAG CCCAGCTAAA 
CTCAATTCAC CAGCCTGCAA GTCTGACGGC CCCTCTGAGC TTCCACTGAC GGATCGAGAA 
ATGGAGATTT TGAACAAGAC AACTGGTATG ACGCAGTCTA CCGAGCAACT CCCTGATTCC 
ATGGATGAAG AAGTTGCTCC TCCCAAGCCC CCTTTACCTG GTATTCGAGT GGTGGATAAT 
AGTCCTCCTC CAGCACTACC ACCCAAAAAG CGCCAATCAG CACCTTCCCC TACGAGAGTG 
GCAGTGGTGG CACCCATGAG TCGAGCCACC AGTGGCTCTA GTTTACCTAT TGGAATTAAT 
AGACAGGATT TTGATGTTGA CTGTTACGCA CAGAGGAGGT TGTCAGGAGG CAGCCACTCG 
TATGGTGGGG AATCCCCCCG CCTCTCCCCC TGCAACAGCA TGGGCAAGCT CAGCAAATCC 
GATGAGCAGC TGTCCTCCTT GGACCGGGAC AGTGGGCAGT GCTCCCGAAA TACAAGCTGT 
GAAACACTAG ATCACTACGA TCCAGATTAT GAATTCCTGC AGGAAGACCT CTCCAATGCT 
GACCAGATAC CTCAACAGGT GCCCTGTAAC CTTAGCCCAT TGCCAGAATC CTTGGGTGAA 
TCTGTTGCTC CATTTTCCGG GAGTCCTTTC AGGCTCTCCC AGAGCAGTTC CCCACAGGCG 
GAGCACTGCA GTCCTCTACC CTCAACACCG TCTTTGGAGA CGCCACCGGC TCTTCCGGAG 
AAGAAACGGA GGAGCGCCGC CTCTCAGCCC TCGGACAGTT CCAGCAGTAG GGTCTCCTAT 
GAGAGGCACC CTTCCCAATA TGACAACATT TCCGAGGACG ACCTGCAGAA CCCAGCCAAC 
ACACAGTCAT TACCTTTCAC GCCGTTTGCT GCCGTTCTCC CCTTTCAGCA GGGGGCTTCT 
TCAGCACCAG GGGAATTTGT TGGGGATTTT GCTGCTTCAG AGTCTTCAGG TGATCCAGAA 
AAACCACCTC CTCTCCCAGA GAAGAAGAAC AAGCACATGT TGGCGTACAT GCAGCTCTTG 
GAGGACTATT CAGAGCCTCA GCCCTCCATG TTCTACCAGA TGCCCCAGAG CGAGCACATT 
TACCAACAGA AGAACAAGTA CTTAATGGAA GTCTATGGAT TCAATGACTC GTTCAGTAGC 
GGCGACTCAC TGCATGAGTT GGCCCCTCCC CCTGCCCTTC CTCCCAAACA GAGACAGCTG 
GCCTCCTATG CTGCTTCTTC CTTCTCCCCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG 
CCCTTCACTC CTGAGGACAC CAGTGCCACT CAAGGCATCA GTATGTCCAT CTCAAACTCC 
TTTCTCAACC GGCACAGTTC CTTTCCTGTG CACTCGTATA AGTCTGTGTT TAGGTCCTAC 
TCCCAGGATT TCGTGCCTCA CAACCAAGCC TCTGTTCCAC CTTTTCTGTC GTCTACCTCC 
TCTTCCACTC CACATTTTCC GCCTGTCCAC CACTCTCAGA GCTCTGACCT AGTGGTGGTG 
CCAACCGCAG CCAACCCGCC TCCAAGCACT GTGGATGGGC CCACCTCCTC TTCTCAGGAG 
AACCGCTTTA ATGGGAATGC GTCTGTCTGC CTTCCTTCTG AACCTCTCAC TGATTCTCTC 
CAGACGTCTT CGGAATCACC AGTTGCCAAA GATGGGCACC CTAAAGATTC CTCAACACAC 
ACCAATGCCA CTGGGAAAGA AAGCCGAGAA GGTGGTGAAA GGCAACAAAA TTCACCAGAT 
GCTTTGGAAT CAGCACAGTC AGAGGAGGAG GTTGATGAGC TCTCCTTGAT TGACCACAGT 
GAAATTATGT CAAGATTGAC ACTTAAGCAA GAGGGTGACG ATGGGCCAGA TGTGCGTGGC 
GGATCTGGGG ATATCTTGTT GGTCCATGCA ACCCAGACTG ACAGAAAAGA TTTGGTTGTG 
TACTGTGAAG CCTTTTTGAC AACGTACAGG ACGTTCATCA CCCCCGAGGA GCTCATCAAA 
AAGCTGCAGT ACAGATATGA GAAGTTCTGC CCGTTCCCTG ATACATTTAA GAAGCGTGTC 
AGCAAAAATA CTTTCTTTGT GCTGGTGCGA GTGGTGGATG AATTGTGTCT AGTGGAATTG 
ACAGAAGAGA TTTTAAAGCT ACTGATGGAG CTTGTGTTTC GGTTAGTGTG CAATGGGGAA 
CTCAGCCTTG CCCGAGTGCT GCGGAAAAAC ATCCTAGATA AAGTGGACCA GAAGAAGATG 
CTGAAATGTG CTAACTCGGA TCAGCCCTTA GCTGCGAGAG GTGTGGCAGC CAGACCAGGA 
ACCCTCCACG ATTTTCATAG CCATGAAATA GCAGAACAGC TAACGCTGTT AGACGCCGAA 
CTCTTCTATA AAATAGAGAT TCCAGAAGTT TTGCTTTGGG CAAAGGAGCA GAATGAAGAG 
AAGAGCCCCA ACCTGACACA GTTCACTGAG CACTTTAATA ACATGTCCTA CTGGGTCCGA 
TCGATAATAA TGCTACAGGA AAAGGCACAG GATAGGGAGA GGCTGCTCCT GAAATTCATC 
AAGATCATGA AGCACTTGCG AAAGCTGAAT AACTTTAACT CCTATCTGGC CATTCTCTCG 
GCTTTGGACT CCGCACCCAT CAGAAGGCTA GAGTGGCAGA AGCAGACATC AGAGGGCCTA 
GCTGAGTACT GCACTTTGAT TGACAGCTCA TCCTCCTTCC GGGCCTATCG GGCGGCTCTA 
TCAGAAGTAG AACCTCCCTG TATCCCATAC CTGGGGCTGA TCCTGCAGGA CCTCACCTTC 
GTTCACCTTG GAAACCCAGA CTACATTGAT GGGAAAGTAA ATTTCTCTAA ACGCTGGCAA 
CAGTTTAACA TCTTGGACAG CATGAGGCGC TTCCAACAAG TACATTATGA AATCCGGAGA 
AATGACGATA TCGTGAACTT CTTCAACGAT TTCAGTGATC ACCTGGCTGA GGAGGCCCTA 
TGGGAACTCT CTCTTAAAAT CAAACCCCGA AACATACCAC GGCGAAAAAC AGACCGGGAA 
GAAAAAACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028917831.1
CDS	174..3785
Translation	MGNANEKQKPLKQNNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_029061998.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029061998.1

ORF Insert Sequence:

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAAATCTA AGCTGGCTGG AAGAGAAGGA GAAGGAAGTC 
GTCAGTGCAT TGCGCTACTT TAAGACAATT GTGGACAAAA TGGCAATTGA CAAGAAGGTG 
TTGGAGATGC TTCCTGGCTC TGCCAGCAAG GTGCTAGAGG CTATTCTACC CCTAGTGCAG 
TCTGACCCTC GGATTCAACA AAGCTCTGCA ATCTCTTCCT GCTACAGCCG TGTATACCAG 
AGTCTGGCCA ATCTGATTCG CTGGTCTGAT CAAGTGATGT TAGAAGGTGT GAACTTAGAA 
GATAAAGAGA TGGTGACAAC TGTCACAGGC GTCATAAAGG CTGTGCTTGA TGGAGTGAAG 
GAGCTGGTCA GACTTACTAT AGAAAAACAG GAGCATCCAT CCCCCACAAG CCCAGCTAAA 
CTCAATTCAC CAGCCTGCAA GTCTGACGGC CCCTCTGAGC TTCCACTGAC GGATCGAGAA 
ATGGAGATTT TGAACAAGAC AACTGGTATG ACGCAGTCTA CCGAGCAACT CCCTGATTCC 
ATGGATGAAG AAGTTGCTCC TCCCAAGCCC CCTTTACCTG GTATTCGAGT GGTGGATAAT 
AGTCCTCCTC CAGCACTACC ACCCAAAAAG CGCCAATCAG CACCTTCCCC TACGAGAGTG 
GCAGTGGTGG CACCCATGAG TCGAGCCACC AGTGGCTCTA GTTTACCTAT TGGAATTAAT 
AGACAGGATT TTGATGTTGA CTGTTACGCA CAGAGGAGGT TGTCAGGAGG CAGCCACTCG 
TATGGTGGGG AATCCCCCCG CCTCTCCCCC TGCAACAGCA TGGGCAAGCT CAGCAAATCC 
GATGAGCAGC TGTCCTCCTT GGACCGGGAC AGTGGGCAGT GCTCCCGAAA TACAAGCTGT 
GAAACACTAG ATCACTACGA TCCAGATTAT GAATTCCTGC AGGAAGACCT CTCCAATGCT 
GACCAGATAC CTCAACAGGT GCCCTGTAAC CTTAGCCCAT TGCCAGAATC CTTGGGTGAA 
TCTGTTGCTC CATTTTCCGG GAGTCCTTTC AGGCTCTCCC AGAGCAGTTC CCCACAGGCG 
GAGCACTGCA GTCCTCTACC CTCAACACCG TCTTTGGAGA CGCCACCGGC TCTTCCGGAG 
AAGAAACGGA GGAGCGCCGC CTCTCAGCCC TCGGACAGTT CCAGCAGTAG GGTCTCCTAT 
GAGAGGCACC CTTCCCAATA TGACAACATT TCCGAGGACG ACCTGCAGAA CCCAGCCAAC 
ACACAGTCAT TACCTTTCAC GCCGTTTGCT GCCGTTCTCC CCTTTCAGCA GGGGGCTTCT 
TCAGCACCAG GGGAATTTGT TGGGGATTTT GCTGCTTCAG AGTCTTCAGG TGATCCAGAA 
AAACCACCTC CTCTCCCAGA GAAGAAGAAC AAGCACATGT TGGCGTACAT GCAGCTCTTG 
GAGGACTATT CAGAGCCTCA GCCCTCCATG TTCTACCAGA TGCCCCAGAG CGAGCACATT 
TACCAACAGA AGAACAAGTA CTTAATGGAA GTCTATGGAT TCAATGACTC GTTCAGTAGC 
GGCGACTCAC TGCATGAGTT GGCCCCTCCC CCTGCCCTTC CTCCCAAACA GAGACAGCTG 
GCCTCCTATG CTGCTTCTTC CTTCTCCCCT GTCTCCTACT GTGTCCAGCA AACTAAAGTG 
CCCTTCACTC CTGAGGACAC CAGTGCCACT CAAGGCATCA GTATGTCCAT CTCAAACTCC 
TTTCTCAACC GGCACAGTTC CTTTCCTGTG CACTCGTATA AGTCTGTGTT TAGGTCCTAC 
TCCCAGGATT TCGTGCCTCA CAACCAAGCC TCTGTTCCAC CTTTTCTGTC GTCTACCTCC 
TCTTCCACTC CACATTTTCC GCCTGTCCAC CACTCTCAGA GCTCTGACCT AGTGGTGGTG 
CCAACCGCAG CCAACCCGCC TCCAAGCACT GTGGATGGGC CCACCTCCTC TTCTCAGGAG 
AACCGCTTTA ATGGGAATGC GTCTGTCTGC CTTCCTTCTG AACCTCTCAC TGATTCTCTC 
CAGACGTCTT CGGAATCACC AGTTGCCAAA GATGGGCACC CTAAAGATTC CTCAACACAC 
ACCAATGCCA CTGGGAAAGA AAGCCGAGAA GGTGGTGAAA GGCAACAAAA TTCACCAGAT 
GCTTTGGAAT CAGCACAGTC AGAGGAGGAG GTTGATGAGC TCTCCTTGAT TGACCACAGT 
GAAATTATGT CAAGATTGAC ACTTAAGCAA GAGGGTGACG ATGGGCCAGA TGTGCGTGGC 
GGATCTGGGG ATATCTTGTT GGTCCATGCA ACCCAGACTG ACAGAAAAGA TTTGGTTGTG 
TACTGTGAAG CCTTTTTGAC AACGTACAGG ACGTTCATCA CCCCCGAGGA GCTCATCAAA 
AAGCTGCAGT ACAGATATGA GAAGTTCTGC CCGTTCCCTG ATACATTTAA GAAGCGTGTC 
AGCAAAAATA CTTTCTTTGT GCTGGTGCGA GTGGTGGATG AATTGTGTCT AGTGGAATTG 
ACAGAAGAGA TTTTAAAGCT ACTGATGGAG CTTGTGTTTC GGTTAGTGTG CAATGGGGAA 
CTCAGCCTTG CCCGAGTGCT GCGGAAAAAC ATCCTAGATA AAGTGGACCA GAAGAAGATG 
CTGAAATGTG CTAACTCGGA TCAGCCCTTA GCTGCGAGAG GTGTGGCAGC CAGACCAGGA 
ACCCTCCACG ATTTTCATAG CCATGAAATA GCAGAACAGC TAACGCTGTT AGACGCCGAA 
CTCTTCTATA AAATAGAGAT TCCAGAAGTT TTGCTTTGGG CAAAGGAGCA GAATGAAGAG 
AAGAGCCCCA ACCTGACACA GTTCACTGAG CACTTTAATA ACATGTCCTA CTGGGTCCGA 
TCGATAATAA TGCTACAGGA AAAGGCACAG GATAGGGAGA GGCTGCTCCT GAAATTCATC 
AAGATCATGA AGCACTTGCG AAAGCTGAAT AACTTTAACT CCTATCTGGC CATTCTCTCG 
GCTTTGGACT CCGCACCCAT CAGAAGGCTA GAGTGGCAGA AGCAGACATC AGAGGGCCTA 
GCTGAGTACT GCACTTTGAT TGACAGCTCA TCCTCCTTCC GGGCCTATCG GGCGGCTCTA 
TCAGAAGTAG AACCTCCCTG TATCCCATAC CTGGGGCTGA TCCTGCAGGA CCTCACCTTC 
GTTCACCTTG GAAACCCAGA CTACATTGAT GGGAAAGTAA ATTTCTCTAA ACGCTGGCAA 
CAGTTTAACA TCTTGGACAG CATGAGGCGC TTCCAACAAG TACATTATGA AATCCGGAGA 
AATGACGATA TCGTGAACTT CTTCAACGAT TTCAGTGATC ACCTGGCTGA GGAGGCCCTA 
TGGGAACTCT CTCTTAAAAT CAAACCCCGA AACATACCAC GGCGAAAAAC AGACCGGGAA 
GAAAAAACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_029061999.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028917832.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OOc126895
Clone ID Related Accession (Same CDS sequence)	XM_029061999.1
Accession Version	XM_029061999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAGAGGCA ACAGACAGTT TTCTACCAGA GGGCTATGCT 
ACTCCCTTGG ATCTGGAGCA CCAAGCTGTA GAATTTAAAT CCATCAGTGC TGTGATTTCC 
AGGTCTCAGA GGCAGAAGAA TCTAAGCTGG CTGGAAGAGA AGGAGAAGGA AGTCGTCAGT 
GCATTGCGCT ACTTTAAGAC AATTGTGGAC AAAATGGCAA TTGACAAGAA GGTGTTGGAG 
ATGCTTCCTG GCTCTGCCAG CAAGGTGCTA GAGGCTATTC TACCCCTAGT GCAGTCTGAC 
CCTCGGATTC AACAAAGCTC TGCAATCTCT TCCTGCTACA GCCGTGTATA CCAGAGTCTG 
GCCAATCTGA TTCGCTGGTC TGATCAAGTG ATGTTAGAAG GTGTGAACTT AGAAGATAAA 
GAGATGGTGA CAACTGTCAC AGGCGTCATA AAGGCTGTGC TTGATGGAGT GAAGGAGCTG 
GTCAGACTTA CTATAGAAAA ACAGGAGCAT CCATCCCCCA CAAGCCCAGC TAAACTCAAT 
TCACCAGCCT GCAAGTCTGA CGGCCCCTCT GAGCTTCCAC TGACGGATCG AGAAATGGAG 
ATTTTGAACA AGACAACTGG TATGACGCAG TCTACCGAGC AACTCCCTGA TTCCATGGAT 
GAAGAAGTTG CTCCTCCCAA GCCCCCTTTA CCTGGTATTC GAGTGGTGGA TAATAGTCCT 
CCTCCAGCAC TACCACCCAA AAAGCGCCAA TCAGCACCTT CCCCTACGAG AGTGGCAGTG 
GTGGCACCCA TGAGTCGAGC CACCAGTGGC TCTAGTTTAC CTATTGGAAT TAATAGACAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG AGGTTGTCAG GAGGCAGCCA CTCGTATGGT 
GGGGAATCCC CCCGCCTCTC CCCCTGCAAC AGCATGGGCA AGCTCAGCAA ATCCGATGAG 
CAGCTGTCCT CCTTGGACCG GGACAGTGGG CAGTGCTCCC GAAATACAAG CTGTGAAACA 
CTAGATCACT ACGATCCAGA TTATGAATTC CTGCAGGAAG ACCTCTCCAA TGCTGACCAG 
ATACCTCAAC AGGTGCCCTG TAACCTTAGC CCATTGCCAG AATCCTTGGG TGAATCTGTT 
GCTCCATTTT CCGGGAGTCC TTTCAGGCTC TCCCAGAGCA GTTCCCCACA GGCGGAGCAC 
TGCAGTCCTC TACCCTCAAC ACCGTCTTTG GAGACGCCAC CGGCTCTTCC GGAGAAGAAA 
CGGAGGAGCG CCGCCTCTCA GCCCTCGGAC AGTTCCAGCA GTAGGGTCTC CTATGAGAGG 
CACCCTTCCC AATATGACAA CATTTCCGAG GACGACCTGC AGAACCCAGC CAACACACAG 
TCATTACCTT TCACGCCGTT TGCTGCCGTT CTCCCCTTTC AGCAGGGGGC TTCTTCAGCA 
CCAGGGGAAT TTGTTGGGGA TTTTGCTGCT TCAGAGTCTT CAGGTGATCC AGAAAAACCA 
CCTCCTCTCC CAGAGAAGAA GAACAAGCAC ATGTTGGCGT ACATGCAGCT CTTGGAGGAC 
TATTCAGAGC CTCAGCCCTC CATGTTCTAC CAGATGCCCC AGAGCGAGCA CATTTACCAA 
CAGAAGAACA AGTACTTAAT GGAAGTCTAT GGATTCAATG ACTCGTTCAG TAGCGGCGAC 
TCACTGCATG AGTTGGCCCC TCCCCCTGCC CTTCCTCCCA AACAGAGACA GCTGGAATCA 
CCAGTTGCCA AAGATGGGCA CCCTAAAGAT TCCTCAACAC ACACCAATGC CACTGGGAAA 
GAAAGCCGAG AAGGTGGTGA AAGGCAACAA AATTCACCAG ATGCTTTGGA ATCAGCACAG 
TCAGAGGAGG AGGTTGATGA GCTCTCCTTG ATTGACCACA GTGAAATTAT GTCAAGATTG 
ACACTTAAGC AAGAGGGTGA CGATGGGCCA GATGTGCGTG GCGGATCTGG GGATATCTTG 
TTGGTCCATG CAACCCAGAC TGACAGAAAA GATTTGGTTG TGTACTGTGA AGCCTTTTTG 
ACAACGTACA GGACGTTCAT CACCCCCGAG GAGCTCATCA AAAAGCTGCA GTACAGATAT 
GAGAAGTTCT GCCCGTTCCC TGATACATTT AAGAAGCGTG TCAGCAAAAA TACTTTCTTT 
GTGCTGGTGC GAGTGGTGGA TGAATTGTGT CTAGTGGAAT TGACAGAAGA GATTTTAAAG 
CTACTGATGG AGCTTGTGTT TCGGTTAGTG TGCAATGGGG AACTCAGCCT TGCCCGAGTG 
CTGCGGAAAA ACATCCTAGA TAAAGTGGAC CAGAAGAAGA TGCTGAAATG TGCTAACTCG 
GATCAGCCCT TAGCTGCGAG AGGTGTGGCA GCCAGACCAG GAACCCTCCA CGATTTTCAT 
AGCCATGAAA TAGCAGAACA GCTAACGCTG TTAGACGCCG AACTCTTCTA TAAAATAGAG 
ATTCCAGAAG TTTTGCTTTG GGCAAAGGAG CAGAATGAAG AGAAGAGCCC CAACCTGACA 
CAGTTCACTG AGCACTTTAA TAACATGTCC TACTGGGTCC GATCGATAAT AATGCTACAG 
GAAAAGGCAC AGGATAGGGA GAGGCTGCTC CTGAAATTCA TCAAGATCAT GAAGCACTTG 
CGAAAGCTGA ATAACTTTAA CTCCTATCTG GCCATTCTCT CGGCTTTGGA CTCCGCACCC 
ATCAGAAGGC TAGAGTGGCA GAAGCAGACA TCAGAGGGCC TAGCTGAGTA CTGCACTTTG 
ATTGACAGCT CATCCTCCTT CCGGGCCTAT CGGGCGGCTC TATCAGAAGT AGAACCTCCC 
TGTATCCCAT ACCTGGGGCT GATCCTGCAG GACCTCACCT TCGTTCACCT TGGAAACCCA 
GACTACATTG ATGGGAAAGT AAATTTCTCT AAACGCTGGC AACAGTTTAA CATCTTGGAC 
AGCATGAGGC GCTTCCAACA AGTACATTAT GAAATCCGGA GAAATGACGA TATCGTGAAC 
TTCTTCAACG ATTTCAGTGA TCACCTGGCT GAGGAGGCCC TATGGGAACT CTCTCTTAAA 
ATCAAACCCC GAAACATACC ACGGCGAAAA ACAGACCGGG AAGAAAAAAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028917832.1
CDS	170..3463
Translation	MGNANEKQKPLKQNNLYLWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_029061999.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029061999.1

ORF Insert Sequence:

ATGGGGAATG CTAATGAAAA ACAAAAACCT TTGAAACAAA ACAATTTGTA CCTCTGGAAG 
CAAGATTCCC AGCGCTCTCA TCTCTCTTCG TTCACTATGA AGCTGATGGA CAAATTTCAT 
TCTCCCAAAA TAAAGAGGAC ACCATCAAAG AAAGGAAAAC CAGCCGACCT CACTGTGAAA 
ACCCCAGAAA AGCCAGTCAA CAAAGAGGCA ACAGACAGTT TTCTACCAGA GGGCTATGCT 
ACTCCCTTGG ATCTGGAGCA CCAAGCTGTA GAATTTAAAT CCATCAGTGC TGTGATTTCC 
AGGTCTCAGA GGCAGAAGAA TCTAAGCTGG CTGGAAGAGA AGGAGAAGGA AGTCGTCAGT 
GCATTGCGCT ACTTTAAGAC AATTGTGGAC AAAATGGCAA TTGACAAGAA GGTGTTGGAG 
ATGCTTCCTG GCTCTGCCAG CAAGGTGCTA GAGGCTATTC TACCCCTAGT GCAGTCTGAC 
CCTCGGATTC AACAAAGCTC TGCAATCTCT TCCTGCTACA GCCGTGTATA CCAGAGTCTG 
GCCAATCTGA TTCGCTGGTC TGATCAAGTG ATGTTAGAAG GTGTGAACTT AGAAGATAAA 
GAGATGGTGA CAACTGTCAC AGGCGTCATA AAGGCTGTGC TTGATGGAGT GAAGGAGCTG 
GTCAGACTTA CTATAGAAAA ACAGGAGCAT CCATCCCCCA CAAGCCCAGC TAAACTCAAT 
TCACCAGCCT GCAAGTCTGA CGGCCCCTCT GAGCTTCCAC TGACGGATCG AGAAATGGAG 
ATTTTGAACA AGACAACTGG TATGACGCAG TCTACCGAGC AACTCCCTGA TTCCATGGAT 
GAAGAAGTTG CTCCTCCCAA GCCCCCTTTA CCTGGTATTC GAGTGGTGGA TAATAGTCCT 
CCTCCAGCAC TACCACCCAA AAAGCGCCAA TCAGCACCTT CCCCTACGAG AGTGGCAGTG 
GTGGCACCCA TGAGTCGAGC CACCAGTGGC TCTAGTTTAC CTATTGGAAT TAATAGACAG 
GATTTTGATG TTGACTGTTA CGCACAGAGG AGGTTGTCAG GAGGCAGCCA CTCGTATGGT 
GGGGAATCCC CCCGCCTCTC CCCCTGCAAC AGCATGGGCA AGCTCAGCAA ATCCGATGAG 
CAGCTGTCCT CCTTGGACCG GGACAGTGGG CAGTGCTCCC GAAATACAAG CTGTGAAACA 
CTAGATCACT ACGATCCAGA TTATGAATTC CTGCAGGAAG ACCTCTCCAA TGCTGACCAG 
ATACCTCAAC AGGTGCCCTG TAACCTTAGC CCATTGCCAG AATCCTTGGG TGAATCTGTT 
GCTCCATTTT CCGGGAGTCC TTTCAGGCTC TCCCAGAGCA GTTCCCCACA GGCGGAGCAC 
TGCAGTCCTC TACCCTCAAC ACCGTCTTTG GAGACGCCAC CGGCTCTTCC GGAGAAGAAA 
CGGAGGAGCG CCGCCTCTCA GCCCTCGGAC AGTTCCAGCA GTAGGGTCTC CTATGAGAGG 
CACCCTTCCC AATATGACAA CATTTCCGAG GACGACCTGC AGAACCCAGC CAACACACAG 
TCATTACCTT TCACGCCGTT TGCTGCCGTT CTCCCCTTTC AGCAGGGGGC TTCTTCAGCA 
CCAGGGGAAT TTGTTGGGGA TTTTGCTGCT TCAGAGTCTT CAGGTGATCC AGAAAAACCA 
CCTCCTCTCC CAGAGAAGAA GAACAAGCAC ATGTTGGCGT ACATGCAGCT CTTGGAGGAC 
TATTCAGAGC CTCAGCCCTC CATGTTCTAC CAGATGCCCC AGAGCGAGCA CATTTACCAA 
CAGAAGAACA AGTACTTAAT GGAAGTCTAT GGATTCAATG ACTCGTTCAG TAGCGGCGAC 
TCACTGCATG AGTTGGCCCC TCCCCCTGCC CTTCCTCCCA AACAGAGACA GCTGGAATCA 
CCAGTTGCCA AAGATGGGCA CCCTAAAGAT TCCTCAACAC ACACCAATGC CACTGGGAAA 
GAAAGCCGAG AAGGTGGTGA AAGGCAACAA AATTCACCAG ATGCTTTGGA ATCAGCACAG 
TCAGAGGAGG AGGTTGATGA GCTCTCCTTG ATTGACCACA GTGAAATTAT GTCAAGATTG 
ACACTTAAGC AAGAGGGTGA CGATGGGCCA GATGTGCGTG GCGGATCTGG GGATATCTTG 
TTGGTCCATG CAACCCAGAC TGACAGAAAA GATTTGGTTG TGTACTGTGA AGCCTTTTTG 
ACAACGTACA GGACGTTCAT CACCCCCGAG GAGCTCATCA AAAAGCTGCA GTACAGATAT 
GAGAAGTTCT GCCCGTTCCC TGATACATTT AAGAAGCGTG TCAGCAAAAA TACTTTCTTT 
GTGCTGGTGC GAGTGGTGGA TGAATTGTGT CTAGTGGAAT TGACAGAAGA GATTTTAAAG 
CTACTGATGG AGCTTGTGTT TCGGTTAGTG TGCAATGGGG AACTCAGCCT TGCCCGAGTG 
CTGCGGAAAA ACATCCTAGA TAAAGTGGAC CAGAAGAAGA TGCTGAAATG TGCTAACTCG 
GATCAGCCCT TAGCTGCGAG AGGTGTGGCA GCCAGACCAG GAACCCTCCA CGATTTTCAT 
AGCCATGAAA TAGCAGAACA GCTAACGCTG TTAGACGCCG AACTCTTCTA TAAAATAGAG 
ATTCCAGAAG TTTTGCTTTG GGCAAAGGAG CAGAATGAAG AGAAGAGCCC CAACCTGACA 
CAGTTCACTG AGCACTTTAA TAACATGTCC TACTGGGTCC GATCGATAAT AATGCTACAG 
GAAAAGGCAC AGGATAGGGA GAGGCTGCTC CTGAAATTCA TCAAGATCAT GAAGCACTTG 
CGAAAGCTGA ATAACTTTAA CTCCTATCTG GCCATTCTCT CGGCTTTGGA CTCCGCACCC 
ATCAGAAGGC TAGAGTGGCA GAAGCAGACA TCAGAGGGCC TAGCTGAGTA CTGCACTTTG 
ATTGACAGCT CATCCTCCTT CCGGGCCTAT CGGGCGGCTC TATCAGAAGT AGAACCTCCC 
TGTATCCCAT ACCTGGGGCT GATCCTGCAG GACCTCACCT TCGTTCACCT TGGAAACCCA 
GACTACATTG ATGGGAAAGT AAATTTCTCT AAACGCTGGC AACAGTTTAA CATCTTGGAC 
AGCATGAGGC GCTTCCAACA AGTACATTAT GAAATCCGGA GAAATGACGA TATCGTGAAC 
TTCTTCAACG ATTTCAGTGA TCACCTGGCT GAGGAGGCCC TATGGGAACT CTCTCTTAAA 
ATCAAACCCC GAAACATACC ACGGCGAAAA ACAGACCGGG AAGAAAAAAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_007662214.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007660404.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

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CloneID	OOc01210
Clone ID Related Accession (Same CDS sequence)	XM_007662214.1 , XM_007662214.2
Accession Version	XM_007662214.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009097.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAGAGGC AACAGACAGT 
TTTCTACCAG AGGGCTATGC TACTCCCTTG GATCTGGAGC ACCAAGCTGT AGAATTTAAA 
TCCATCAGTG CTGTGATTTC CAGGTCTCAG AGGCAGAAGA ATCTAAGCTG GCTGGAAGAG 
AAGGAGAAGG AAGTCGTCAG TGCATTGCGC TACTTTAAGA CAATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTGTTGGA GATGCTTCCT GGCTCTGCCA GCAAGGTGCT AGAGGCTATT 
CTACCCCTAG TGCAGTCTGA CCCTCGGATT CAACAAAGCT CTGCAATCTC TTCCTGCTAC 
AGCCGTGTAT ACCAGAGTCT GGCCAATCTG ATTCGCTGGT CTGATCAAGT GATGTTAGAA 
GGTGTGAACT TAGAAGATAA AGAGATGGTG ACAACTGTCA CAGGCGTCAT AAAGGCTGTG 
CTTGATGGAG TGAAGGAGCT GGTCAGACTT ACTATAGAAA AACAGGAGCA TCCATCCCCC 
ACAAGCCCAG CTAAACTCAA TTCACCAGCC TGCAAGTCTG ACGGCCCCTC TGAGCTTCCA 
CTGACGGATC GAGAAATGGA GATTTTGAAC AAGACAACTG GTATGACGCA GTCTACCGAG 
CAACTCCCTG ATTCCATGGA TGAAGAAGTT GCTCCTCCCA AGCCCCCTTT ACCTGGTATT 
CGAGTGGTGG ATAATAGTCC TCCTCCAGCA CTACCACCCA AAAAGCGCCA ATCAGCACCT 
TCCCCTACGA GAGTGGCAGT GGTGGCACCC ATGAGTCGAG CCACCAGTGG CTCTAGTTTA 
CCTATTGGAA TTAATAGACA GGATTTTGAT GTTGACTGTT ACGCACAGAG GAGGTTGTCA 
GGAGGCAGCC ACTCGTATGG TGGGGAATCC CCCCGCCTCT CCCCCTGCAA CAGCATGGGC 
AAGCTCAGCA AATCCGATGA GCAGCTGTCC TCCTTGGACC GGGACAGTGG GCAGTGCTCC 
CGAAATACAA GCTGTGAAAC ACTAGATCAC TACGATCCAG ATTATGAATT CCTGCAGGAA 
GACCTCTCCA ATGCTGACCA GATACCTCAA CAGGTGCCCT GTAACCTTAG CCCATTGCCA 
GAATCCTTGG GTGAATCTGT TGCTCCATTT TCCGGGAGTC CTTTCAGGCT CTCCCAGAGC 
AGTTCCCCAC AGGCGGAGCA CTGCAGTCCT CTACCCTCAA CACCGTCTTT GGAGACGCCA 
CCGGCTCTTC CGGAGAAGAA ACGGAGGAGC GCCGCCTCTC AGCCCTCGGA CAGTTCCAGC 
AGTAGGGTCT CCTATGAGAG GCACCCTTCC CAATATGACA ACATTTCCGA GGACGACCTG 
CAGAACCCAG CCAACACACA GTCATTACCT TTCACGCCGT TTGCTGCCGT TCTCCCCTTT 
CAGCAGGGGG CTTCTTCAGC ACCAGGGGAA TTTGTTGGGG ATTTTGCTGC TTCAGAGTCT 
TCAGGTGATC CAGAAAAACC ACCTCCTCTC CCAGAGAAGA AGAACAAGCA CATGTTGGCG 
TACATGCAGC TCTTGGAGGA CTATTCAGAG CCTCAGCCCT CCATGTTCTA CCAGATGCCC 
CAGAGCGAGC ACATTTACCA ACAGAAGAAC AAGTACTTAA TGGAAGTCTA TGGATTCAAT 
GACTCGTTCA GTAGCGGCGA CTCACTGCAT GAGTTGGCCC CTCCCCCTGC CCTTCCTCCC 
AAACAGAGAC AGCTGCAGGC CTCCTATGCT GCTTCTTCCT TCTCCCCTGT CTCCTACTGT 
GTCCAGCAAA CTAAAGTGCC CTTCACTCCT GAGGACACCA GTGCCACTCA AGGCATCAGT 
ATGTCCATCT CAAACTCCTT TCTCAACCGG CACAGTTCCT TTCCTGTGCA CTCGTATAAG 
TCTGTGTTTA GGTCCTACTC CCAGGATTTC GTGCCTCACA ACCAAGCCTC TGTTCCACCT 
TTTCTGTCGT CTACCTCCTC TTCCACTCCA CATTTTCCGC CTGTCCACCA CTCTCAGAGC 
TCTGACCTAG TGGTGGTGCC AACCGCAGCC AACCCGCCTC CAAGCACTGT GGATGGGCCC 
ACCTCCTCTT CTCAGGAGAA CCGCTTTAAT GGGAATGCGT CTGTCTGCCT TCCTTCTGAA 
CCTCTCACTG ATTCTCTCCA GACGTCTTCG GAATCACCAG TTGCCAAAGA TGGGCACCCT 
AAAGATTCCT CAACACACAC CAATGCCACT GGGAAAGAAA GCCGAGAAGG TGGTGAAAGG 
CAACAAAATT CACCAGATGC TTTGGAATCA GCACAGTCAG AGGAGGAGGT TGATGAGCTC 
TCCTTGATTG ACCACAGTGA AATTATGTCA AGATTGACAC TTAAGCAAGA GGGTGACGAT 
GGGCCAGATG TGCGTGGCGG ATCTGGGGAT ATCTTGTTGG TCCATGCAAC CCAGACTGAC 
AGAAAAGATT TGGTTGTGTA CTGTGAAGCC TTTTTGACAA CGTACAGGAC GTTCATCACC 
CCCGAGGAGC TCATCAAAAA GCTGCAGTAC AGATATGAGA AGTTCTGCCC GTTCCCTGAT 
ACATTTAAGA AGCGTGTCAG CAAAAATACT TTCTTTGTGC TGGTGCGAGT GGTGGATGAA 
TTGTGTCTAG TGGAATTGAC AGAAGAGATT TTAAAGCTAC TGATGGAGCT TGTGTTTCGG 
TTAGTGTGCA ATGGGGAACT CAGCCTTGCC CGAGTGCTGC GGAAAAACAT CCTAGATAAA 
GTGGACCAGA AGAAGATGCT GAAATGTGCT AACTCGGATC AGCCCTTAGC TGCGAGAGGT 
GTGGCAGCCA GACCAGGAAC CCTCCACGAT TTTCATAGCC ATGAAATAGC AGAACAGCTA 
ACGCTGTTAG ACGCCGAACT CTTCTATAAA ATAGAGATTC CAGAAGTTTT GCTTTGGGCA 
AAGGAGCAGA ATGAAGAGAA GAGCCCCAAC CTGACACAGT TCACTGAGCA CTTTAATAAC 
ATGTCCTACT GGGTCCGATC GATAATAATG CTACAGGAAA AGGCACAGGA TAGGGAGAGG 
CTGCTCCTGA AATTCATCAA GATCATGAAG CACTTGCGAA AGCTGAATAA CTTTAACTCC 
TATCTGGCCA TTCTCTCGGC TTTGGACTCC GCACCCATCA GAAGGCTAGA GTGGCAGAAG 
CAGACATCAG AGGGCCTAGC TGAGTACTGC ACTTTGATTG ACAGCTCATC CTCCTTCCGG 
GCCTATCGGG CGGCTCTATC AGAAGTAGAA CCTCCCTGTA TCCCATACCT GGGGCTGATC 
CTGCAGGACC TCACCTTCGT TCACCTTGGA AACCCAGACT ACATTGATGG GAAAGTAAAT 
TTCTCTAAAC GCTGGCAACA GTTTAACATC TTGGACAGCA TGAGGCGCTT CCAACAAGTA 
CATTATGAAA TCCGGAGAAA TGACGATATC GTGAACTTCT TCAACGATTT CAGTGATCAC 
CTGGCTGAGG AGGCCCTATG GGAACTCTCT CTTAAAATCA AACCCCGAAA CATACCACGG 
CGAAAAACAG ACCGGGAAGA AAAAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007660404.1
CDS	32..3721
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLQASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_007662214.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007662214.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAGAGGC AACAGACAGT 
TTTCTACCAG AGGGCTATGC TACTCCCTTG GATCTGGAGC ACCAAGCTGT AGAATTTAAA 
TCCATCAGTG CTGTGATTTC CAGGTCTCAG AGGCAGAAGA ATCTAAGCTG GCTGGAAGAG 
AAGGAGAAGG AAGTCGTCAG TGCATTGCGC TACTTTAAGA CAATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTGTTGGA GATGCTTCCT GGCTCTGCCA GCAAGGTGCT AGAGGCTATT 
CTACCCCTAG TGCAGTCTGA CCCTCGGATT CAACAAAGCT CTGCAATCTC TTCCTGCTAC 
AGCCGTGTAT ACCAGAGTCT GGCCAATCTG ATTCGCTGGT CTGATCAAGT GATGTTAGAA 
GGTGTGAACT TAGAAGATAA AGAGATGGTG ACAACTGTCA CAGGCGTCAT AAAGGCTGTG 
CTTGATGGAG TGAAGGAGCT GGTCAGACTT ACTATAGAAA AACAGGAGCA TCCATCCCCC 
ACAAGCCCAG CTAAACTCAA TTCACCAGCC TGCAAGTCTG ACGGCCCCTC TGAGCTTCCA 
CTGACGGATC GAGAAATGGA GATTTTGAAC AAGACAACTG GTATGACGCA GTCTACCGAG 
CAACTCCCTG ATTCCATGGA TGAAGAAGTT GCTCCTCCCA AGCCCCCTTT ACCTGGTATT 
CGAGTGGTGG ATAATAGTCC TCCTCCAGCA CTACCACCCA AAAAGCGCCA ATCAGCACCT 
TCCCCTACGA GAGTGGCAGT GGTGGCACCC ATGAGTCGAG CCACCAGTGG CTCTAGTTTA 
CCTATTGGAA TTAATAGACA GGATTTTGAT GTTGACTGTT ACGCACAGAG GAGGTTGTCA 
GGAGGCAGCC ACTCGTATGG TGGGGAATCC CCCCGCCTCT CCCCCTGCAA CAGCATGGGC 
AAGCTCAGCA AATCCGATGA GCAGCTGTCC TCCTTGGACC GGGACAGTGG GCAGTGCTCC 
CGAAATACAA GCTGTGAAAC ACTAGATCAC TACGATCCAG ATTATGAATT CCTGCAGGAA 
GACCTCTCCA ATGCTGACCA GATACCTCAA CAGGTGCCCT GTAACCTTAG CCCATTGCCA 
GAATCCTTGG GTGAATCTGT TGCTCCATTT TCCGGGAGTC CTTTCAGGCT CTCCCAGAGC 
AGTTCCCCAC AGGCGGAGCA CTGCAGTCCT CTACCCTCAA CACCGTCTTT GGAGACGCCA 
CCGGCTCTTC CGGAGAAGAA ACGGAGGAGC GCCGCCTCTC AGCCCTCGGA CAGTTCCAGC 
AGTAGGGTCT CCTATGAGAG GCACCCTTCC CAATATGACA ACATTTCCGA GGACGACCTG 
CAGAACCCAG CCAACACACA GTCATTACCT TTCACGCCGT TTGCTGCCGT TCTCCCCTTT 
CAGCAGGGGG CTTCTTCAGC ACCAGGGGAA TTTGTTGGGG ATTTTGCTGC TTCAGAGTCT 
TCAGGTGATC CAGAAAAACC ACCTCCTCTC CCAGAGAAGA AGAACAAGCA CATGTTGGCG 
TACATGCAGC TCTTGGAGGA CTATTCAGAG CCTCAGCCCT CCATGTTCTA CCAGATGCCC 
CAGAGCGAGC ACATTTACCA ACAGAAGAAC AAGTACTTAA TGGAAGTCTA TGGATTCAAT 
GACTCGTTCA GTAGCGGCGA CTCACTGCAT GAGTTGGCCC CTCCCCCTGC CCTTCCTCCC 
AAACAGAGAC AGCTGCAGGC CTCCTATGCT GCTTCTTCCT TCTCCCCTGT CTCCTACTGT 
GTCCAGCAAA CTAAAGTGCC CTTCACTCCT GAGGACACCA GTGCCACTCA AGGCATCAGT 
ATGTCCATCT CAAACTCCTT TCTCAACCGG CACAGTTCCT TTCCTGTGCA CTCGTATAAG 
TCTGTGTTTA GGTCCTACTC CCAGGATTTC GTGCCTCACA ACCAAGCCTC TGTTCCACCT 
TTTCTGTCGT CTACCTCCTC TTCCACTCCA CATTTTCCGC CTGTCCACCA CTCTCAGAGC 
TCTGACCTAG TGGTGGTGCC AACCGCAGCC AACCCGCCTC CAAGCACTGT GGATGGGCCC 
ACCTCCTCTT CTCAGGAGAA CCGCTTTAAT GGGAATGCGT CTGTCTGCCT TCCTTCTGAA 
CCTCTCACTG ATTCTCTCCA GACGTCTTCG GAATCACCAG TTGCCAAAGA TGGGCACCCT 
AAAGATTCCT CAACACACAC CAATGCCACT GGGAAAGAAA GCCGAGAAGG TGGTGAAAGG 
CAACAAAATT CACCAGATGC TTTGGAATCA GCACAGTCAG AGGAGGAGGT TGATGAGCTC 
TCCTTGATTG ACCACAGTGA AATTATGTCA AGATTGACAC TTAAGCAAGA GGGTGACGAT 
GGGCCAGATG TGCGTGGCGG ATCTGGGGAT ATCTTGTTGG TCCATGCAAC CCAGACTGAC 
AGAAAAGATT TGGTTGTGTA CTGTGAAGCC TTTTTGACAA CGTACAGGAC GTTCATCACC 
CCCGAGGAGC TCATCAAAAA GCTGCAGTAC AGATATGAGA AGTTCTGCCC GTTCCCTGAT 
ACATTTAAGA AGCGTGTCAG CAAAAATACT TTCTTTGTGC TGGTGCGAGT GGTGGATGAA 
TTGTGTCTAG TGGAATTGAC AGAAGAGATT TTAAAGCTAC TGATGGAGCT TGTGTTTCGG 
TTAGTGTGCA ATGGGGAACT CAGCCTTGCC CGAGTGCTGC GGAAAAACAT CCTAGATAAA 
GTGGACCAGA AGAAGATGCT GAAATGTGCT AACTCGGATC AGCCCTTAGC TGCGAGAGGT 
GTGGCAGCCA GACCAGGAAC CCTCCACGAT TTTCATAGCC ATGAAATAGC AGAACAGCTA 
ACGCTGTTAG ACGCCGAACT CTTCTATAAA ATAGAGATTC CAGAAGTTTT GCTTTGGGCA 
AAGGAGCAGA ATGAAGAGAA GAGCCCCAAC CTGACACAGT TCACTGAGCA CTTTAATAAC 
ATGTCCTACT GGGTCCGATC GATAATAATG CTACAGGAAA AGGCACAGGA TAGGGAGAGG 
CTGCTCCTGA AATTCATCAA GATCATGAAG CACTTGCGAA AGCTGAATAA CTTTAACTCC 
TATCTGGCCA TTCTCTCGGC TTTGGACTCC GCACCCATCA GAAGGCTAGA GTGGCAGAAG 
CAGACATCAG AGGGCCTAGC TGAGTACTGC ACTTTGATTG ACAGCTCATC CTCCTTCCGG 
GCCTATCGGG CGGCTCTATC AGAAGTAGAA CCTCCCTGTA TCCCATACCT GGGGCTGATC 
CTGCAGGACC TCACCTTCGT TCACCTTGGA AACCCAGACT ACATTGATGG GAAAGTAAAT 
TTCTCTAAAC GCTGGCAACA GTTTAACATC TTGGACAGCA TGAGGCGCTT CCAACAAGTA 
CATTATGAAA TCCGGAGAAA TGACGATATC GTGAACTTCT TCAACGATTT CAGTGATCAC 
CTGGCTGAGG AGGCCCTATG GGAACTCTCT CTTAAAATCA AACCCCGAAA CATACCACGG 
CGAAAAACAG ACCGGGAAGA AAAAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_007662214.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007660404.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOc01210
Clone ID Related Accession (Same CDS sequence)	XM_007662214.1 , XM_007662214.2
Accession Version	XM_007662214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 29, 2019 this sequence version replaced XM_007662214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAGAGGC AACAGACAGT 
TTTCTACCAG AGGGCTATGC TACTCCCTTG GATCTGGAGC ACCAAGCTGT AGAATTTAAA 
TCCATCAGTG CTGTGATTTC CAGGTCTCAG AGGCAGAAGA ATCTAAGCTG GCTGGAAGAG 
AAGGAGAAGG AAGTCGTCAG TGCATTGCGC TACTTTAAGA CAATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTGTTGGA GATGCTTCCT GGCTCTGCCA GCAAGGTGCT AGAGGCTATT 
CTACCCCTAG TGCAGTCTGA CCCTCGGATT CAACAAAGCT CTGCAATCTC TTCCTGCTAC 
AGCCGTGTAT ACCAGAGTCT GGCCAATCTG ATTCGCTGGT CTGATCAAGT GATGTTAGAA 
GGTGTGAACT TAGAAGATAA AGAGATGGTG ACAACTGTCA CAGGCGTCAT AAAGGCTGTG 
CTTGATGGAG TGAAGGAGCT GGTCAGACTT ACTATAGAAA AACAGGAGCA TCCATCCCCC 
ACAAGCCCAG CTAAACTCAA TTCACCAGCC TGCAAGTCTG ACGGCCCCTC TGAGCTTCCA 
CTGACGGATC GAGAAATGGA GATTTTGAAC AAGACAACTG GTATGACGCA GTCTACCGAG 
CAACTCCCTG ATTCCATGGA TGAAGAAGTT GCTCCTCCCA AGCCCCCTTT ACCTGGTATT 
CGAGTGGTGG ATAATAGTCC TCCTCCAGCA CTACCACCCA AAAAGCGCCA ATCAGCACCT 
TCCCCTACGA GAGTGGCAGT GGTGGCACCC ATGAGTCGAG CCACCAGTGG CTCTAGTTTA 
CCTATTGGAA TTAATAGACA GGATTTTGAT GTTGACTGTT ACGCACAGAG GAGGTTGTCA 
GGAGGCAGCC ACTCGTATGG TGGGGAATCC CCCCGCCTCT CCCCCTGCAA CAGCATGGGC 
AAGCTCAGCA AATCCGATGA GCAGCTGTCC TCCTTGGACC GGGACAGTGG GCAGTGCTCC 
CGAAATACAA GCTGTGAAAC ACTAGATCAC TACGATCCAG ATTATGAATT CCTGCAGGAA 
GACCTCTCCA ATGCTGACCA GATACCTCAA CAGGTGCCCT GTAACCTTAG CCCATTGCCA 
GAATCCTTGG GTGAATCTGT TGCTCCATTT TCCGGGAGTC CTTTCAGGCT CTCCCAGAGC 
AGTTCCCCAC AGGCGGAGCA CTGCAGTCCT CTACCCTCAA CACCGTCTTT GGAGACGCCA 
CCGGCTCTTC CGGAGAAGAA ACGGAGGAGC GCCGCCTCTC AGCCCTCGGA CAGTTCCAGC 
AGTAGGGTCT CCTATGAGAG GCACCCTTCC CAATATGACA ACATTTCCGA GGACGACCTG 
CAGAACCCAG CCAACACACA GTCATTACCT TTCACGCCGT TTGCTGCCGT TCTCCCCTTT 
CAGCAGGGGG CTTCTTCAGC ACCAGGGGAA TTTGTTGGGG ATTTTGCTGC TTCAGAGTCT 
TCAGGTGATC CAGAAAAACC ACCTCCTCTC CCAGAGAAGA AGAACAAGCA CATGTTGGCG 
TACATGCAGC TCTTGGAGGA CTATTCAGAG CCTCAGCCCT CCATGTTCTA CCAGATGCCC 
CAGAGCGAGC ACATTTACCA ACAGAAGAAC AAGTACTTAA TGGAAGTCTA TGGATTCAAT 
GACTCGTTCA GTAGCGGCGA CTCACTGCAT GAGTTGGCCC CTCCCCCTGC CCTTCCTCCC 
AAACAGAGAC AGCTGCAGGC CTCCTATGCT GCTTCTTCCT TCTCCCCTGT CTCCTACTGT 
GTCCAGCAAA CTAAAGTGCC CTTCACTCCT GAGGACACCA GTGCCACTCA AGGCATCAGT 
ATGTCCATCT CAAACTCCTT TCTCAACCGG CACAGTTCCT TTCCTGTGCA CTCGTATAAG 
TCTGTGTTTA GGTCCTACTC CCAGGATTTC GTGCCTCACA ACCAAGCCTC TGTTCCACCT 
TTTCTGTCGT CTACCTCCTC TTCCACTCCA CATTTTCCGC CTGTCCACCA CTCTCAGAGC 
TCTGACCTAG TGGTGGTGCC AACCGCAGCC AACCCGCCTC CAAGCACTGT GGATGGGCCC 
ACCTCCTCTT CTCAGGAGAA CCGCTTTAAT GGGAATGCGT CTGTCTGCCT TCCTTCTGAA 
CCTCTCACTG ATTCTCTCCA GACGTCTTCG GAATCACCAG TTGCCAAAGA TGGGCACCCT 
AAAGATTCCT CAACACACAC CAATGCCACT GGGAAAGAAA GCCGAGAAGG TGGTGAAAGG 
CAACAAAATT CACCAGATGC TTTGGAATCA GCACAGTCAG AGGAGGAGGT TGATGAGCTC 
TCCTTGATTG ACCACAGTGA AATTATGTCA AGATTGACAC TTAAGCAAGA GGGTGACGAT 
GGGCCAGATG TGCGTGGCGG ATCTGGGGAT ATCTTGTTGG TCCATGCAAC CCAGACTGAC 
AGAAAAGATT TGGTTGTGTA CTGTGAAGCC TTTTTGACAA CGTACAGGAC GTTCATCACC 
CCCGAGGAGC TCATCAAAAA GCTGCAGTAC AGATATGAGA AGTTCTGCCC GTTCCCTGAT 
ACATTTAAGA AGCGTGTCAG CAAAAATACT TTCTTTGTGC TGGTGCGAGT GGTGGATGAA 
TTGTGTCTAG TGGAATTGAC AGAAGAGATT TTAAAGCTAC TGATGGAGCT TGTGTTTCGG 
TTAGTGTGCA ATGGGGAACT CAGCCTTGCC CGAGTGCTGC GGAAAAACAT CCTAGATAAA 
GTGGACCAGA AGAAGATGCT GAAATGTGCT AACTCGGATC AGCCCTTAGC TGCGAGAGGT 
GTGGCAGCCA GACCAGGAAC CCTCCACGAT TTTCATAGCC ATGAAATAGC AGAACAGCTA 
ACGCTGTTAG ACGCCGAACT CTTCTATAAA ATAGAGATTC CAGAAGTTTT GCTTTGGGCA 
AAGGAGCAGA ATGAAGAGAA GAGCCCCAAC CTGACACAGT TCACTGAGCA CTTTAATAAC 
ATGTCCTACT GGGTCCGATC GATAATAATG CTACAGGAAA AGGCACAGGA TAGGGAGAGG 
CTGCTCCTGA AATTCATCAA GATCATGAAG CACTTGCGAA AGCTGAATAA CTTTAACTCC 
TATCTGGCCA TTCTCTCGGC TTTGGACTCC GCACCCATCA GAAGGCTAGA GTGGCAGAAG 
CAGACATCAG AGGGCCTAGC TGAGTACTGC ACTTTGATTG ACAGCTCATC CTCCTTCCGG 
GCCTATCGGG CGGCTCTATC AGAAGTAGAA CCTCCCTGTA TCCCATACCT GGGGCTGATC 
CTGCAGGACC TCACCTTCGT TCACCTTGGA AACCCAGACT ACATTGATGG GAAAGTAAAT 
TTCTCTAAAC GCTGGCAACA GTTTAACATC TTGGACAGCA TGAGGCGCTT CCAACAAGTA 
CATTATGAAA TCCGGAGAAA TGACGATATC GTGAACTTCT TCAACGATTT CAGTGATCAC 
CTGGCTGAGG AGGCCCTATG GGAACTCTCT CTTAAAATCA AACCCCGAAA CATACCACGG 
CGAAAAACAG ACCGGGAAGA AAAAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007660404.1
CDS	28..3717
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLQASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_007662214.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007662214.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAGAGGC AACAGACAGT 
TTTCTACCAG AGGGCTATGC TACTCCCTTG GATCTGGAGC ACCAAGCTGT AGAATTTAAA 
TCCATCAGTG CTGTGATTTC CAGGTCTCAG AGGCAGAAGA ATCTAAGCTG GCTGGAAGAG 
AAGGAGAAGG AAGTCGTCAG TGCATTGCGC TACTTTAAGA CAATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTGTTGGA GATGCTTCCT GGCTCTGCCA GCAAGGTGCT AGAGGCTATT 
CTACCCCTAG TGCAGTCTGA CCCTCGGATT CAACAAAGCT CTGCAATCTC TTCCTGCTAC 
AGCCGTGTAT ACCAGAGTCT GGCCAATCTG ATTCGCTGGT CTGATCAAGT GATGTTAGAA 
GGTGTGAACT TAGAAGATAA AGAGATGGTG ACAACTGTCA CAGGCGTCAT AAAGGCTGTG 
CTTGATGGAG TGAAGGAGCT GGTCAGACTT ACTATAGAAA AACAGGAGCA TCCATCCCCC 
ACAAGCCCAG CTAAACTCAA TTCACCAGCC TGCAAGTCTG ACGGCCCCTC TGAGCTTCCA 
CTGACGGATC GAGAAATGGA GATTTTGAAC AAGACAACTG GTATGACGCA GTCTACCGAG 
CAACTCCCTG ATTCCATGGA TGAAGAAGTT GCTCCTCCCA AGCCCCCTTT ACCTGGTATT 
CGAGTGGTGG ATAATAGTCC TCCTCCAGCA CTACCACCCA AAAAGCGCCA ATCAGCACCT 
TCCCCTACGA GAGTGGCAGT GGTGGCACCC ATGAGTCGAG CCACCAGTGG CTCTAGTTTA 
CCTATTGGAA TTAATAGACA GGATTTTGAT GTTGACTGTT ACGCACAGAG GAGGTTGTCA 
GGAGGCAGCC ACTCGTATGG TGGGGAATCC CCCCGCCTCT CCCCCTGCAA CAGCATGGGC 
AAGCTCAGCA AATCCGATGA GCAGCTGTCC TCCTTGGACC GGGACAGTGG GCAGTGCTCC 
CGAAATACAA GCTGTGAAAC ACTAGATCAC TACGATCCAG ATTATGAATT CCTGCAGGAA 
GACCTCTCCA ATGCTGACCA GATACCTCAA CAGGTGCCCT GTAACCTTAG CCCATTGCCA 
GAATCCTTGG GTGAATCTGT TGCTCCATTT TCCGGGAGTC CTTTCAGGCT CTCCCAGAGC 
AGTTCCCCAC AGGCGGAGCA CTGCAGTCCT CTACCCTCAA CACCGTCTTT GGAGACGCCA 
CCGGCTCTTC CGGAGAAGAA ACGGAGGAGC GCCGCCTCTC AGCCCTCGGA CAGTTCCAGC 
AGTAGGGTCT CCTATGAGAG GCACCCTTCC CAATATGACA ACATTTCCGA GGACGACCTG 
CAGAACCCAG CCAACACACA GTCATTACCT TTCACGCCGT TTGCTGCCGT TCTCCCCTTT 
CAGCAGGGGG CTTCTTCAGC ACCAGGGGAA TTTGTTGGGG ATTTTGCTGC TTCAGAGTCT 
TCAGGTGATC CAGAAAAACC ACCTCCTCTC CCAGAGAAGA AGAACAAGCA CATGTTGGCG 
TACATGCAGC TCTTGGAGGA CTATTCAGAG CCTCAGCCCT CCATGTTCTA CCAGATGCCC 
CAGAGCGAGC ACATTTACCA ACAGAAGAAC AAGTACTTAA TGGAAGTCTA TGGATTCAAT 
GACTCGTTCA GTAGCGGCGA CTCACTGCAT GAGTTGGCCC CTCCCCCTGC CCTTCCTCCC 
AAACAGAGAC AGCTGCAGGC CTCCTATGCT GCTTCTTCCT TCTCCCCTGT CTCCTACTGT 
GTCCAGCAAA CTAAAGTGCC CTTCACTCCT GAGGACACCA GTGCCACTCA AGGCATCAGT 
ATGTCCATCT CAAACTCCTT TCTCAACCGG CACAGTTCCT TTCCTGTGCA CTCGTATAAG 
TCTGTGTTTA GGTCCTACTC CCAGGATTTC GTGCCTCACA ACCAAGCCTC TGTTCCACCT 
TTTCTGTCGT CTACCTCCTC TTCCACTCCA CATTTTCCGC CTGTCCACCA CTCTCAGAGC 
TCTGACCTAG TGGTGGTGCC AACCGCAGCC AACCCGCCTC CAAGCACTGT GGATGGGCCC 
ACCTCCTCTT CTCAGGAGAA CCGCTTTAAT GGGAATGCGT CTGTCTGCCT TCCTTCTGAA 
CCTCTCACTG ATTCTCTCCA GACGTCTTCG GAATCACCAG TTGCCAAAGA TGGGCACCCT 
AAAGATTCCT CAACACACAC CAATGCCACT GGGAAAGAAA GCCGAGAAGG TGGTGAAAGG 
CAACAAAATT CACCAGATGC TTTGGAATCA GCACAGTCAG AGGAGGAGGT TGATGAGCTC 
TCCTTGATTG ACCACAGTGA AATTATGTCA AGATTGACAC TTAAGCAAGA GGGTGACGAT 
GGGCCAGATG TGCGTGGCGG ATCTGGGGAT ATCTTGTTGG TCCATGCAAC CCAGACTGAC 
AGAAAAGATT TGGTTGTGTA CTGTGAAGCC TTTTTGACAA CGTACAGGAC GTTCATCACC 
CCCGAGGAGC TCATCAAAAA GCTGCAGTAC AGATATGAGA AGTTCTGCCC GTTCCCTGAT 
ACATTTAAGA AGCGTGTCAG CAAAAATACT TTCTTTGTGC TGGTGCGAGT GGTGGATGAA 
TTGTGTCTAG TGGAATTGAC AGAAGAGATT TTAAAGCTAC TGATGGAGCT TGTGTTTCGG 
TTAGTGTGCA ATGGGGAACT CAGCCTTGCC CGAGTGCTGC GGAAAAACAT CCTAGATAAA 
GTGGACCAGA AGAAGATGCT GAAATGTGCT AACTCGGATC AGCCCTTAGC TGCGAGAGGT 
GTGGCAGCCA GACCAGGAAC CCTCCACGAT TTTCATAGCC ATGAAATAGC AGAACAGCTA 
ACGCTGTTAG ACGCCGAACT CTTCTATAAA ATAGAGATTC CAGAAGTTTT GCTTTGGGCA 
AAGGAGCAGA ATGAAGAGAA GAGCCCCAAC CTGACACAGT TCACTGAGCA CTTTAATAAC 
ATGTCCTACT GGGTCCGATC GATAATAATG CTACAGGAAA AGGCACAGGA TAGGGAGAGG 
CTGCTCCTGA AATTCATCAA GATCATGAAG CACTTGCGAA AGCTGAATAA CTTTAACTCC 
TATCTGGCCA TTCTCTCGGC TTTGGACTCC GCACCCATCA GAAGGCTAGA GTGGCAGAAG 
CAGACATCAG AGGGCCTAGC TGAGTACTGC ACTTTGATTG ACAGCTCATC CTCCTTCCGG 
GCCTATCGGG CGGCTCTATC AGAAGTAGAA CCTCCCTGTA TCCCATACCT GGGGCTGATC 
CTGCAGGACC TCACCTTCGT TCACCTTGGA AACCCAGACT ACATTGATGG GAAAGTAAAT 
TTCTCTAAAC GCTGGCAACA GTTTAACATC TTGGACAGCA TGAGGCGCTT CCAACAAGTA 
CATTATGAAA TCCGGAGAAA TGACGATATC GTGAACTTCT TCAACGATTT CAGTGATCAC 
CTGGCTGAGG AGGCCCTATG GGAACTCTCT CTTAAAATCA AACCCCGAAA CATACCACGG 
CGAAAAACAG ACCGGGAAGA AAAAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_007662218.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007660408.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc01211
Clone ID Related Accession (Same CDS sequence)	XM_007662218.1
Accession Version	XM_007662218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009097.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAGAGGC AACAGACAGT 
TTTCTACCAG AGGGCTATGC TACTCCCTTG GATCTGGAGC ACCAAGCTGT AGAATTTAAA 
TCCATCAGTG CTGTGATTTC CAGGTCTCAG AGGCAGAAGA ATCTAAGCTG GCTGGAAGAG 
AAGGAGAAGG AAGTCGTCAG TGCATTGCGC TACTTTAAGA CAATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTGTTGGA GATGCTTCCT GGCTCTGCCA GCAAGGTGCT AGAGGCTATT 
CTACCCCTAG TGCAGTCTGA CCCTCGGATT CAACAAAGCT CTGCAATCTC TTCCTGCTAC 
AGCCGTGTAT ACCAGAGTCT GGCCAATCTG ATTCGCTGGT CTGATCAAGT GATGTTAGAA 
GGTGTGAACT TAGAAGATAA AGAGATGGTG ACAACTGTCA CAGGCGTCAT AAAGGCTGTG 
CTTGATGGAG TGAAGGAGCT GGTCAGACTT ACTATAGAAA AACAGGAGCA TCCATCCCCC 
ACAAGCCCAG CTAAACTCAA TTCACCAGCC TGCAAGTCTG ACGGCCCCTC TGAGCTTCCA 
CTGACGGATC GAGAAATGGA GATTTTGAAC AAGACAACTG GTATGACGCA GTCTACCGAG 
CAACTCCCTG ATTCCATGGA TGAAGAAGTT GCTCCTCCCA AGCCCCCTTT ACCTGGTATT 
CGAGTGGTGG ATAATAGTCC TCCTCCAGCA CTACCACCCA AAAAGCGCCA ATCAGCACCT 
TCCCCTACGA GAGTGGCAGT GGTGGCACCC ATGAGTCGAG CCACCAGTGG CTCTAGTTTA 
CCTATTGGAA TTAATAGACA GGATTTTGAT GTTGACTGTT ACGCACAGAG GAGGTTGTCA 
GGAGGCAGCC ACTCGTATGG TGGGGAATCC CCCCGCCTCT CCCCCTGCAA CAGCATGGGC 
AAGCTCAGCA AATCCGATGA GCAGCTGTCC TCCTTGGACC GGGACAGTGG GCAGTGCTCC 
CGAAATACAA GCTGTGAAAC ACTAGATCAC TACGATCCAG ATTATGAATT CCTGCAGGAA 
GACCTCTCCA ATGCTGACCA GATACCTCAA CAGGTGCCCT GTAACCTTAG CCCATTGCCA 
GAATCCTTGG GTGAATCTGT TGCTCCATTT TCCGGGAGTC CTTTCAGGCT CTCCCAGAGC 
AGTTCCCCAC AGGCGGAGCA CTGCAGTCCT CTACCCTCAA CACCGTCTTT GGAGACGCCA 
CCGGCTCTTC CGGAGAAGAA ACGGAGGAGC GCCGCCTCTC AGCCCTCGGA CAGTTCCAGC 
AGTAGGGTCT CCTATGAGAG GCACCCTTCC CAATATGACA ACATTTCCGA GGACGACCTG 
CAGAACCCAG CCAACACACA GTCATTACCT TTCACGCCGT TTGCTGCCGT TCTCCCCTTT 
CAGCAGGGGG CTTCTTCAGC ACCAGGGGAA TTTGTTGGGG ATTTTGCTGC TTCAGAGTCT 
TCAGGTGATC CAGAAAAACC ACCTCCTCTC CCAGAGAAGA AGAACAAGCA CATGTTGGCG 
TACATGCAGC TCTTGGAGGA CTATTCAGAG CCTCAGCCCT CCATGTTCTA CCAGATGCCC 
CAGAGCGAGC ACATTTACCA ACAGAAGAAC AAGTACTTAA TGGAAGTCTA TGGATTCAAT 
GACTCGTTCA GTAGCGGCGA CTCACTGCAT GAGTTGGCCC CTCCCCCTGC CCTTCCTCCC 
AAACAGAGAC AGCTGGCCTC CTATGCTGCT TCTTCCTTCT CCCCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGCCCTT CACTCCTGAG GACACCAGTG CCACTCAAGG CATCAGTATG 
TCCATCTCAA ACTCCTTTCT CAACCGGCAC AGTTCCTTTC CTGTGCACTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACAACC AAGCCTCTGT TCCACCTTTT 
CTGTCGTCTA CCTCCTCTTC CACTCCACAT TTTCCGCCTG TCCACCACTC TCAGAGCTCT 
GACCTAGTGG TGGTGCCAAC CGCAGCCAAC CCGCCTCCAA GCACTGTGGA TGGGCCCACC 
TCCTCTTCTC AGGAGAACCG CTTTAATGGG AATGCGTCTG TCTGCCTTCC TTCTGAACCT 
CTCACTGATT CTCTCCAGAC GTCTTCGGAA TCACCAGTTG CCAAAGATGG GCACCCTAAA 
GATTCCTCAA CACACACCAA TGCCACTGGG AAAGAAAGCC GAGAAGGTGG TGAAAGGCAA 
CAAAATTCAC CAGATGCTTT GGAATCAGCA CAGTCAGAGG AGGAGGTTGA TGAGCTCTCC 
TTGATTGACC ACAGTGAAAT TATGTCAAGA TTGACACTTA AGCAAGAGGG TGACGATGGG 
CCAGATGTGC GTGGCGGATC TGGGGATATC TTGTTGGTCC ATGCAACCCA GACTGACAGA 
AAAGATTTGG TTGTGTACTG TGAAGCCTTT TTGACAACGT ACAGGACGTT CATCACCCCC 
GAGGAGCTCA TCAAAAAGCT GCAGTACAGA TATGAGAAGT TCTGCCCGTT CCCTGATACA 
TTTAAGAAGC GTGTCAGCAA AAATACTTTC TTTGTGCTGG TGCGAGTGGT GGATGAATTG 
TGTCTAGTGG AATTGACAGA AGAGATTTTA AAGCTACTGA TGGAGCTTGT GTTTCGGTTA 
GTGTGCAATG GGGAACTCAG CCTTGCCCGA GTGCTGCGGA AAAACATCCT AGATAAAGTG 
GACCAGAAGA AGATGCTGAA ATGTGCTAAC TCGGATCAGC CCTTAGCTGC GAGAGGTGTG 
GCAGCCAGAC CAGGAACCCT CCACGATTTT CATAGCCATG AAATAGCAGA ACAGCTAACG 
CTGTTAGACG CCGAACTCTT CTATAAAATA GAGATTCCAG AAGTTTTGCT TTGGGCAAAG 
GAGCAGAATG AAGAGAAGAG CCCCAACCTG ACACAGTTCA CTGAGCACTT TAATAACATG 
TCCTACTGGG TCCGATCGAT AATAATGCTA CAGGAAAAGG CACAGGATAG GGAGAGGCTG 
CTCCTGAAAT TCATCAAGAT CATGAAGCAC TTGCGAAAGC TGAATAACTT TAACTCCTAT 
CTGGCCATTC TCTCGGCTTT GGACTCCGCA CCCATCAGAA GGCTAGAGTG GCAGAAGCAG 
ACATCAGAGG GCCTAGCTGA GTACTGCACT TTGATTGACA GCTCATCCTC CTTCCGGGCC 
TATCGGGCGG CTCTATCAGA AGTAGAACCT CCCTGTATCC CATACCTGGG GCTGATCCTG 
CAGGACCTCA CCTTCGTTCA CCTTGGAAAC CCAGACTACA TTGATGGGAA AGTAAATTTC 
TCTAAACGCT GGCAACAGTT TAACATCTTG GACAGCATGA GGCGCTTCCA ACAAGTACAT 
TATGAAATCC GGAGAAATGA CGATATCGTG AACTTCTTCA ACGATTTCAG TGATCACCTG 
GCTGAGGAGG CCCTATGGGA ACTCTCTCTT AAAATCAAAC CCCGAAACAT ACCACGGCGA 
AAAACAGACC GGGAAGAAAA AACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007660408.1
CDS	32..3718
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKEATDSFLPEGYATPLDLEHQAVEFKSISAVISRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_007662218.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007662218.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAGAGGC AACAGACAGT 
TTTCTACCAG AGGGCTATGC TACTCCCTTG GATCTGGAGC ACCAAGCTGT AGAATTTAAA 
TCCATCAGTG CTGTGATTTC CAGGTCTCAG AGGCAGAAGA ATCTAAGCTG GCTGGAAGAG 
AAGGAGAAGG AAGTCGTCAG TGCATTGCGC TACTTTAAGA CAATTGTGGA CAAAATGGCA 
ATTGACAAGA AGGTGTTGGA GATGCTTCCT GGCTCTGCCA GCAAGGTGCT AGAGGCTATT 
CTACCCCTAG TGCAGTCTGA CCCTCGGATT CAACAAAGCT CTGCAATCTC TTCCTGCTAC 
AGCCGTGTAT ACCAGAGTCT GGCCAATCTG ATTCGCTGGT CTGATCAAGT GATGTTAGAA 
GGTGTGAACT TAGAAGATAA AGAGATGGTG ACAACTGTCA CAGGCGTCAT AAAGGCTGTG 
CTTGATGGAG TGAAGGAGCT GGTCAGACTT ACTATAGAAA AACAGGAGCA TCCATCCCCC 
ACAAGCCCAG CTAAACTCAA TTCACCAGCC TGCAAGTCTG ACGGCCCCTC TGAGCTTCCA 
CTGACGGATC GAGAAATGGA GATTTTGAAC AAGACAACTG GTATGACGCA GTCTACCGAG 
CAACTCCCTG ATTCCATGGA TGAAGAAGTT GCTCCTCCCA AGCCCCCTTT ACCTGGTATT 
CGAGTGGTGG ATAATAGTCC TCCTCCAGCA CTACCACCCA AAAAGCGCCA ATCAGCACCT 
TCCCCTACGA GAGTGGCAGT GGTGGCACCC ATGAGTCGAG CCACCAGTGG CTCTAGTTTA 
CCTATTGGAA TTAATAGACA GGATTTTGAT GTTGACTGTT ACGCACAGAG GAGGTTGTCA 
GGAGGCAGCC ACTCGTATGG TGGGGAATCC CCCCGCCTCT CCCCCTGCAA CAGCATGGGC 
AAGCTCAGCA AATCCGATGA GCAGCTGTCC TCCTTGGACC GGGACAGTGG GCAGTGCTCC 
CGAAATACAA GCTGTGAAAC ACTAGATCAC TACGATCCAG ATTATGAATT CCTGCAGGAA 
GACCTCTCCA ATGCTGACCA GATACCTCAA CAGGTGCCCT GTAACCTTAG CCCATTGCCA 
GAATCCTTGG GTGAATCTGT TGCTCCATTT TCCGGGAGTC CTTTCAGGCT CTCCCAGAGC 
AGTTCCCCAC AGGCGGAGCA CTGCAGTCCT CTACCCTCAA CACCGTCTTT GGAGACGCCA 
CCGGCTCTTC CGGAGAAGAA ACGGAGGAGC GCCGCCTCTC AGCCCTCGGA CAGTTCCAGC 
AGTAGGGTCT CCTATGAGAG GCACCCTTCC CAATATGACA ACATTTCCGA GGACGACCTG 
CAGAACCCAG CCAACACACA GTCATTACCT TTCACGCCGT TTGCTGCCGT TCTCCCCTTT 
CAGCAGGGGG CTTCTTCAGC ACCAGGGGAA TTTGTTGGGG ATTTTGCTGC TTCAGAGTCT 
TCAGGTGATC CAGAAAAACC ACCTCCTCTC CCAGAGAAGA AGAACAAGCA CATGTTGGCG 
TACATGCAGC TCTTGGAGGA CTATTCAGAG CCTCAGCCCT CCATGTTCTA CCAGATGCCC 
CAGAGCGAGC ACATTTACCA ACAGAAGAAC AAGTACTTAA TGGAAGTCTA TGGATTCAAT 
GACTCGTTCA GTAGCGGCGA CTCACTGCAT GAGTTGGCCC CTCCCCCTGC CCTTCCTCCC 
AAACAGAGAC AGCTGGCCTC CTATGCTGCT TCTTCCTTCT CCCCTGTCTC CTACTGTGTC 
CAGCAAACTA AAGTGCCCTT CACTCCTGAG GACACCAGTG CCACTCAAGG CATCAGTATG 
TCCATCTCAA ACTCCTTTCT CAACCGGCAC AGTTCCTTTC CTGTGCACTC GTATAAGTCT 
GTGTTTAGGT CCTACTCCCA GGATTTCGTG CCTCACAACC AAGCCTCTGT TCCACCTTTT 
CTGTCGTCTA CCTCCTCTTC CACTCCACAT TTTCCGCCTG TCCACCACTC TCAGAGCTCT 
GACCTAGTGG TGGTGCCAAC CGCAGCCAAC CCGCCTCCAA GCACTGTGGA TGGGCCCACC 
TCCTCTTCTC AGGAGAACCG CTTTAATGGG AATGCGTCTG TCTGCCTTCC TTCTGAACCT 
CTCACTGATT CTCTCCAGAC GTCTTCGGAA TCACCAGTTG CCAAAGATGG GCACCCTAAA 
GATTCCTCAA CACACACCAA TGCCACTGGG AAAGAAAGCC GAGAAGGTGG TGAAAGGCAA 
CAAAATTCAC CAGATGCTTT GGAATCAGCA CAGTCAGAGG AGGAGGTTGA TGAGCTCTCC 
TTGATTGACC ACAGTGAAAT TATGTCAAGA TTGACACTTA AGCAAGAGGG TGACGATGGG 
CCAGATGTGC GTGGCGGATC TGGGGATATC TTGTTGGTCC ATGCAACCCA GACTGACAGA 
AAAGATTTGG TTGTGTACTG TGAAGCCTTT TTGACAACGT ACAGGACGTT CATCACCCCC 
GAGGAGCTCA TCAAAAAGCT GCAGTACAGA TATGAGAAGT TCTGCCCGTT CCCTGATACA 
TTTAAGAAGC GTGTCAGCAA AAATACTTTC TTTGTGCTGG TGCGAGTGGT GGATGAATTG 
TGTCTAGTGG AATTGACAGA AGAGATTTTA AAGCTACTGA TGGAGCTTGT GTTTCGGTTA 
GTGTGCAATG GGGAACTCAG CCTTGCCCGA GTGCTGCGGA AAAACATCCT AGATAAAGTG 
GACCAGAAGA AGATGCTGAA ATGTGCTAAC TCGGATCAGC CCTTAGCTGC GAGAGGTGTG 
GCAGCCAGAC CAGGAACCCT CCACGATTTT CATAGCCATG AAATAGCAGA ACAGCTAACG 
CTGTTAGACG CCGAACTCTT CTATAAAATA GAGATTCCAG AAGTTTTGCT TTGGGCAAAG 
GAGCAGAATG AAGAGAAGAG CCCCAACCTG ACACAGTTCA CTGAGCACTT TAATAACATG 
TCCTACTGGG TCCGATCGAT AATAATGCTA CAGGAAAAGG CACAGGATAG GGAGAGGCTG 
CTCCTGAAAT TCATCAAGAT CATGAAGCAC TTGCGAAAGC TGAATAACTT TAACTCCTAT 
CTGGCCATTC TCTCGGCTTT GGACTCCGCA CCCATCAGAA GGCTAGAGTG GCAGAAGCAG 
ACATCAGAGG GCCTAGCTGA GTACTGCACT TTGATTGACA GCTCATCCTC CTTCCGGGCC 
TATCGGGCGG CTCTATCAGA AGTAGAACCT CCCTGTATCC CATACCTGGG GCTGATCCTG 
CAGGACCTCA CCTTCGTTCA CCTTGGAAAC CCAGACTACA TTGATGGGAA AGTAAATTTC 
TCTAAACGCT GGCAACAGTT TAACATCTTG GACAGCATGA GGCGCTTCCA ACAAGTACAT 
TATGAAATCC GGAGAAATGA CGATATCGTG AACTTCTTCA ACGATTTCAG TGATCACCTG 
GCTGAGGAGG CCCTATGGGA ACTCTCTCTT AAAATCAAAC CCCGAAACAT ACCACGGCGA 
AAAACAGACC GGGAAGAAAA AACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_007662219.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007660409.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

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CloneID	OOc01212
Clone ID Related Accession (Same CDS sequence)	XM_007662219.1
Accession Version	XM_007662219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009097.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAAATCT AAGCTGGCTG 
GAAGAGAAGG AGAAGGAAGT CGTCAGTGCA TTGCGCTACT TTAAGACAAT TGTGGACAAA 
ATGGCAATTG ACAAGAAGGT GTTGGAGATG CTTCCTGGCT CTGCCAGCAA GGTGCTAGAG 
GCTATTCTAC CCCTAGTGCA GTCTGACCCT CGGATTCAAC AAAGCTCTGC AATCTCTTCC 
TGCTACAGCC GTGTATACCA GAGTCTGGCC AATCTGATTC GCTGGTCTGA TCAAGTGATG 
TTAGAAGGTG TGAACTTAGA AGATAAAGAG ATGGTGACAA CTGTCACAGG CGTCATAAAG 
GCTGTGCTTG ATGGAGTGAA GGAGCTGGTC AGACTTACTA TAGAAAAACA GGAGCATCCA 
TCCCCCACAA GCCCAGCTAA ACTCAATTCA CCAGCCTGCA AGTCTGACGG CCCCTCTGAG 
CTTCCACTGA CGGATCGAGA AATGGAGATT TTGAACAAGA CAACTGGTAT GACGCAGTCT 
ACCGAGCAAC TCCCTGATTC CATGGATGAA GAAGTTGCTC CTCCCAAGCC CCCTTTACCT 
GGTATTCGAG TGGTGGATAA TAGTCCTCCT CCAGCACTAC CACCCAAAAA GCGCCAATCA 
GCACCTTCCC CTACGAGAGT GGCAGTGGTG GCACCCATGA GTCGAGCCAC CAGTGGCTCT 
AGTTTACCTA TTGGAATTAA TAGACAGGAT TTTGATGTTG ACTGTTACGC ACAGAGGAGG 
TTGTCAGGAG GCAGCCACTC GTATGGTGGG GAATCCCCCC GCCTCTCCCC CTGCAACAGC 
ATGGGCAAGC TCAGCAAATC CGATGAGCAG CTGTCCTCCT TGGACCGGGA CAGTGGGCAG 
TGCTCCCGAA ATACAAGCTG TGAAACACTA GATCACTACG ATCCAGATTA TGAATTCCTG 
CAGGAAGACC TCTCCAATGC TGACCAGATA CCTCAACAGG TGCCCTGTAA CCTTAGCCCA 
TTGCCAGAAT CCTTGGGTGA ATCTGTTGCT CCATTTTCCG GGAGTCCTTT CAGGCTCTCC 
CAGAGCAGTT CCCCACAGGC GGAGCACTGC AGTCCTCTAC CCTCAACACC GTCTTTGGAG 
ACGCCACCGG CTCTTCCGGA GAAGAAACGG AGGAGCGCCG CCTCTCAGCC CTCGGACAGT 
TCCAGCAGTA GGGTCTCCTA TGAGAGGCAC CCTTCCCAAT ATGACAACAT TTCCGAGGAC 
GACCTGCAGA ACCCAGCCAA CACACAGTCA TTACCTTTCA CGCCGTTTGC TGCCGTTCTC 
CCCTTTCAGC AGGGGGCTTC TTCAGCACCA GGGGAATTTG TTGGGGATTT TGCTGCTTCA 
GAGTCTTCAG GTGATCCAGA AAAACCACCT CCTCTCCCAG AGAAGAAGAA CAAGCACATG 
TTGGCGTACA TGCAGCTCTT GGAGGACTAT TCAGAGCCTC AGCCCTCCAT GTTCTACCAG 
ATGCCCCAGA GCGAGCACAT TTACCAACAG AAGAACAAGT ACTTAATGGA AGTCTATGGA 
TTCAATGACT CGTTCAGTAG CGGCGACTCA CTGCATGAGT TGGCCCCTCC CCCTGCCCTT 
CCTCCCAAAC AGAGACAGCT GCAGGCCTCC TATGCTGCTT CTTCCTTCTC CCCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGCCCTTC ACTCCTGAGG ACACCAGTGC CACTCAAGGC 
ATCAGTATGT CCATCTCAAA CTCCTTTCTC AACCGGCACA GTTCCTTTCC TGTGCACTCG 
TATAAGTCTG TGTTTAGGTC CTACTCCCAG GATTTCGTGC CTCACAACCA AGCCTCTGTT 
CCACCTTTTC TGTCGTCTAC CTCCTCTTCC ACTCCACATT TTCCGCCTGT CCACCACTCT 
CAGAGCTCTG ACCTAGTGGT GGTGCCAACC GCAGCCAACC CGCCTCCAAG CACTGTGGAT 
GGGCCCACCT CCTCTTCTCA GGAGAACCGC TTTAATGGGA ATGCGTCTGT CTGCCTTCCT 
TCTGAACCTC TCACTGATTC TCTCCAGACG TCTTCGGAAT CACCAGTTGC CAAAGATGGG 
CACCCTAAAG ATTCCTCAAC ACACACCAAT GCCACTGGGA AAGAAAGCCG AGAAGGTGGT 
GAAAGGCAAC AAAATTCACC AGATGCTTTG GAATCAGCAC AGTCAGAGGA GGAGGTTGAT 
GAGCTCTCCT TGATTGACCA CAGTGAAATT ATGTCAAGAT TGACACTTAA GCAAGAGGGT 
GACGATGGGC CAGATGTGCG TGGCGGATCT GGGGATATCT TGTTGGTCCA TGCAACCCAG 
ACTGACAGAA AAGATTTGGT TGTGTACTGT GAAGCCTTTT TGACAACGTA CAGGACGTTC 
ATCACCCCCG AGGAGCTCAT CAAAAAGCTG CAGTACAGAT ATGAGAAGTT CTGCCCGTTC 
CCTGATACAT TTAAGAAGCG TGTCAGCAAA AATACTTTCT TTGTGCTGGT GCGAGTGGTG 
GATGAATTGT GTCTAGTGGA ATTGACAGAA GAGATTTTAA AGCTACTGAT GGAGCTTGTG 
TTTCGGTTAG TGTGCAATGG GGAACTCAGC CTTGCCCGAG TGCTGCGGAA AAACATCCTA 
GATAAAGTGG ACCAGAAGAA GATGCTGAAA TGTGCTAACT CGGATCAGCC CTTAGCTGCG 
AGAGGTGTGG CAGCCAGACC AGGAACCCTC CACGATTTTC ATAGCCATGA AATAGCAGAA 
CAGCTAACGC TGTTAGACGC CGAACTCTTC TATAAAATAG AGATTCCAGA AGTTTTGCTT 
TGGGCAAAGG AGCAGAATGA AGAGAAGAGC CCCAACCTGA CACAGTTCAC TGAGCACTTT 
AATAACATGT CCTACTGGGT CCGATCGATA ATAATGCTAC AGGAAAAGGC ACAGGATAGG 
GAGAGGCTGC TCCTGAAATT CATCAAGATC ATGAAGCACT TGCGAAAGCT GAATAACTTT 
AACTCCTATC TGGCCATTCT CTCGGCTTTG GACTCCGCAC CCATCAGAAG GCTAGAGTGG 
CAGAAGCAGA CATCAGAGGG CCTAGCTGAG TACTGCACTT TGATTGACAG CTCATCCTCC 
TTCCGGGCCT ATCGGGCGGC TCTATCAGAA GTAGAACCTC CCTGTATCCC ATACCTGGGG 
CTGATCCTGC AGGACCTCAC CTTCGTTCAC CTTGGAAACC CAGACTACAT TGATGGGAAA 
GTAAATTTCT CTAAACGCTG GCAACAGTTT AACATCTTGG ACAGCATGAG GCGCTTCCAA 
CAAGTACATT ATGAAATCCG GAGAAATGAC GATATCGTGA ACTTCTTCAA CGATTTCAGT 
GATCACCTGG CTGAGGAGGC CCTATGGGAA CTCTCTCTTA AAATCAAACC CCGAAACATA 
CCACGGCGAA AAACAGACCG GGAAGAAAAA ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007660409.1
CDS	32..3607
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLQASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_007662219.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007662219.1

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAAATCT AAGCTGGCTG 
GAAGAGAAGG AGAAGGAAGT CGTCAGTGCA TTGCGCTACT TTAAGACAAT TGTGGACAAA 
ATGGCAATTG ACAAGAAGGT GTTGGAGATG CTTCCTGGCT CTGCCAGCAA GGTGCTAGAG 
GCTATTCTAC CCCTAGTGCA GTCTGACCCT CGGATTCAAC AAAGCTCTGC AATCTCTTCC 
TGCTACAGCC GTGTATACCA GAGTCTGGCC AATCTGATTC GCTGGTCTGA TCAAGTGATG 
TTAGAAGGTG TGAACTTAGA AGATAAAGAG ATGGTGACAA CTGTCACAGG CGTCATAAAG 
GCTGTGCTTG ATGGAGTGAA GGAGCTGGTC AGACTTACTA TAGAAAAACA GGAGCATCCA 
TCCCCCACAA GCCCAGCTAA ACTCAATTCA CCAGCCTGCA AGTCTGACGG CCCCTCTGAG 
CTTCCACTGA CGGATCGAGA AATGGAGATT TTGAACAAGA CAACTGGTAT GACGCAGTCT 
ACCGAGCAAC TCCCTGATTC CATGGATGAA GAAGTTGCTC CTCCCAAGCC CCCTTTACCT 
GGTATTCGAG TGGTGGATAA TAGTCCTCCT CCAGCACTAC CACCCAAAAA GCGCCAATCA 
GCACCTTCCC CTACGAGAGT GGCAGTGGTG GCACCCATGA GTCGAGCCAC CAGTGGCTCT 
AGTTTACCTA TTGGAATTAA TAGACAGGAT TTTGATGTTG ACTGTTACGC ACAGAGGAGG 
TTGTCAGGAG GCAGCCACTC GTATGGTGGG GAATCCCCCC GCCTCTCCCC CTGCAACAGC 
ATGGGCAAGC TCAGCAAATC CGATGAGCAG CTGTCCTCCT TGGACCGGGA CAGTGGGCAG 
TGCTCCCGAA ATACAAGCTG TGAAACACTA GATCACTACG ATCCAGATTA TGAATTCCTG 
CAGGAAGACC TCTCCAATGC TGACCAGATA CCTCAACAGG TGCCCTGTAA CCTTAGCCCA 
TTGCCAGAAT CCTTGGGTGA ATCTGTTGCT CCATTTTCCG GGAGTCCTTT CAGGCTCTCC 
CAGAGCAGTT CCCCACAGGC GGAGCACTGC AGTCCTCTAC CCTCAACACC GTCTTTGGAG 
ACGCCACCGG CTCTTCCGGA GAAGAAACGG AGGAGCGCCG CCTCTCAGCC CTCGGACAGT 
TCCAGCAGTA GGGTCTCCTA TGAGAGGCAC CCTTCCCAAT ATGACAACAT TTCCGAGGAC 
GACCTGCAGA ACCCAGCCAA CACACAGTCA TTACCTTTCA CGCCGTTTGC TGCCGTTCTC 
CCCTTTCAGC AGGGGGCTTC TTCAGCACCA GGGGAATTTG TTGGGGATTT TGCTGCTTCA 
GAGTCTTCAG GTGATCCAGA AAAACCACCT CCTCTCCCAG AGAAGAAGAA CAAGCACATG 
TTGGCGTACA TGCAGCTCTT GGAGGACTAT TCAGAGCCTC AGCCCTCCAT GTTCTACCAG 
ATGCCCCAGA GCGAGCACAT TTACCAACAG AAGAACAAGT ACTTAATGGA AGTCTATGGA 
TTCAATGACT CGTTCAGTAG CGGCGACTCA CTGCATGAGT TGGCCCCTCC CCCTGCCCTT 
CCTCCCAAAC AGAGACAGCT GCAGGCCTCC TATGCTGCTT CTTCCTTCTC CCCTGTCTCC 
TACTGTGTCC AGCAAACTAA AGTGCCCTTC ACTCCTGAGG ACACCAGTGC CACTCAAGGC 
ATCAGTATGT CCATCTCAAA CTCCTTTCTC AACCGGCACA GTTCCTTTCC TGTGCACTCG 
TATAAGTCTG TGTTTAGGTC CTACTCCCAG GATTTCGTGC CTCACAACCA AGCCTCTGTT 
CCACCTTTTC TGTCGTCTAC CTCCTCTTCC ACTCCACATT TTCCGCCTGT CCACCACTCT 
CAGAGCTCTG ACCTAGTGGT GGTGCCAACC GCAGCCAACC CGCCTCCAAG CACTGTGGAT 
GGGCCCACCT CCTCTTCTCA GGAGAACCGC TTTAATGGGA ATGCGTCTGT CTGCCTTCCT 
TCTGAACCTC TCACTGATTC TCTCCAGACG TCTTCGGAAT CACCAGTTGC CAAAGATGGG 
CACCCTAAAG ATTCCTCAAC ACACACCAAT GCCACTGGGA AAGAAAGCCG AGAAGGTGGT 
GAAAGGCAAC AAAATTCACC AGATGCTTTG GAATCAGCAC AGTCAGAGGA GGAGGTTGAT 
GAGCTCTCCT TGATTGACCA CAGTGAAATT ATGTCAAGAT TGACACTTAA GCAAGAGGGT 
GACGATGGGC CAGATGTGCG TGGCGGATCT GGGGATATCT TGTTGGTCCA TGCAACCCAG 
ACTGACAGAA AAGATTTGGT TGTGTACTGT GAAGCCTTTT TGACAACGTA CAGGACGTTC 
ATCACCCCCG AGGAGCTCAT CAAAAAGCTG CAGTACAGAT ATGAGAAGTT CTGCCCGTTC 
CCTGATACAT TTAAGAAGCG TGTCAGCAAA AATACTTTCT TTGTGCTGGT GCGAGTGGTG 
GATGAATTGT GTCTAGTGGA ATTGACAGAA GAGATTTTAA AGCTACTGAT GGAGCTTGTG 
TTTCGGTTAG TGTGCAATGG GGAACTCAGC CTTGCCCGAG TGCTGCGGAA AAACATCCTA 
GATAAAGTGG ACCAGAAGAA GATGCTGAAA TGTGCTAACT CGGATCAGCC CTTAGCTGCG 
AGAGGTGTGG CAGCCAGACC AGGAACCCTC CACGATTTTC ATAGCCATGA AATAGCAGAA 
CAGCTAACGC TGTTAGACGC CGAACTCTTC TATAAAATAG AGATTCCAGA AGTTTTGCTT 
TGGGCAAAGG AGCAGAATGA AGAGAAGAGC CCCAACCTGA CACAGTTCAC TGAGCACTTT 
AATAACATGT CCTACTGGGT CCGATCGATA ATAATGCTAC AGGAAAAGGC ACAGGATAGG 
GAGAGGCTGC TCCTGAAATT CATCAAGATC ATGAAGCACT TGCGAAAGCT GAATAACTTT 
AACTCCTATC TGGCCATTCT CTCGGCTTTG GACTCCGCAC CCATCAGAAG GCTAGAGTGG 
CAGAAGCAGA CATCAGAGGG CCTAGCTGAG TACTGCACTT TGATTGACAG CTCATCCTCC 
TTCCGGGCCT ATCGGGCGGC TCTATCAGAA GTAGAACCTC CCTGTATCCC ATACCTGGGG 
CTGATCCTGC AGGACCTCAC CTTCGTTCAC CTTGGAAACC CAGACTACAT TGATGGGAAA 
GTAAATTTCT CTAAACGCTG GCAACAGTTT AACATCTTGG ACAGCATGAG GCGCTTCCAA 
CAAGTACATT ATGAAATCCG GAGAAATGAC GATATCGTGA ACTTCTTCAA CGATTTCAGT 
GATCACCTGG CTGAGGAGGC CCTATGGGAA CTCTCTCTTA AAATCAAACC CCGAAACATA 
CCACGGCGAA AAACAGACCG GGAAGAAAAA ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_007662220.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007660410.1 Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

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CloneID	OOc01213
Clone ID Related Accession (Same CDS sequence)	XM_007662220.2
Accession Version	XM_007662220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009097.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 15, 2016 this sequence version replaced XM_007662220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAAATCT AAGCTGGCTG 
GAAGAGAAGG AGAAGGAAGT CGTCAGTGCA TTGCGCTACT TTAAGACAAT TGTGGACAAA 
ATGGCAATTG ACAAGAAGGT GTTGGAGATG CTTCCTGGCT CTGCCAGCAA GGTGCTAGAG 
GCTATTCTAC CCCTAGTGCA GTCTGACCCT CGGATTCAAC AAAGCTCTGC AATCTCTTCC 
TGCTACAGCC GTGTATACCA GAGTCTGGCC AATCTGATTC GCTGGTCTGA TCAAGTGATG 
TTAGAAGGTG TGAACTTAGA AGATAAAGAG ATGGTGACAA CTGTCACAGG CGTCATAAAG 
GCTGTGCTTG ATGGAGTGAA GGAGCTGGTC AGACTTACTA TAGAAAAACA GGAGCATCCA 
TCCCCCACAA GCCCAGCTAA ACTCAATTCA CCAGCCTGCA AGTCTGACGG CCCCTCTGAG 
CTTCCACTGA CGGATCGAGA AATGGAGATT TTGAACAAGA CAACTGGTAT GACGCAGTCT 
ACCGAGCAAC TCCCTGATTC CATGGATGAA GAAGTTGCTC CTCCCAAGCC CCCTTTACCT 
GGTATTCGAG TGGTGGATAA TAGTCCTCCT CCAGCACTAC CACCCAAAAA GCGCCAATCA 
GCACCTTCCC CTACGAGAGT GGCAGTGGTG GCACCCATGA GTCGAGCCAC CAGTGGCTCT 
AGTTTACCTA TTGGAATTAA TAGACAGGAT TTTGATGTTG ACTGTTACGC ACAGAGGAGG 
TTGTCAGGAG GCAGCCACTC GTATGGTGGG GAATCCCCCC GCCTCTCCCC CTGCAACAGC 
ATGGGCAAGC TCAGCAAATC CGATGAGCAG CTGTCCTCCT TGGACCGGGA CAGTGGGCAG 
TGCTCCCGAA ATACAAGCTG TGAAACACTA GATCACTACG ATCCAGATTA TGAATTCCTG 
CAGGAAGACC TCTCCAATGC TGACCAGATA CCTCAACAGG TGCCCTGTAA CCTTAGCCCA 
TTGCCAGAAT CCTTGGGTGA ATCTGTTGCT CCATTTTCCG GGAGTCCTTT CAGGCTCTCC 
CAGAGCAGTT CCCCACAGGC GGAGCACTGC AGTCCTCTAC CCTCAACACC GTCTTTGGAG 
ACGCCACCGG CTCTTCCGGA GAAGAAACGG AGGAGCGCCG CCTCTCAGCC CTCGGACAGT 
TCCAGCAGTA GGGTCTCCTA TGAGAGGCAC CCTTCCCAAT ATGACAACAT TTCCGAGGAC 
GACCTGCAGA ACCCAGCCAA CACACAGTCA TTACCTTTCA CGCCGTTTGC TGCCGTTCTC 
CCCTTTCAGC AGGGGGCTTC TTCAGCACCA GGGGAATTTG TTGGGGATTT TGCTGCTTCA 
GAGTCTTCAG GTGATCCAGA AAAACCACCT CCTCTCCCAG AGAAGAAGAA CAAGCACATG 
TTGGCGTACA TGCAGCTCTT GGAGGACTAT TCAGAGCCTC AGCCCTCCAT GTTCTACCAG 
ATGCCCCAGA GCGAGCACAT TTACCAACAG AAGAACAAGT ACTTAATGGA AGTCTATGGA 
TTCAATGACT CGTTCAGTAG CGGCGACTCA CTGCATGAGT TGGCCCCTCC CCCTGCCCTT 
CCTCCCAAAC AGAGACAGCT GGCCTCCTAT GCTGCTTCTT CCTTCTCCCC TGTCTCCTAC 
TGTGTCCAGC AAACTAAAGT GCCCTTCACT CCTGAGGACA CCAGTGCCAC TCAAGGCATC 
AGTATGTCCA TCTCAAACTC CTTTCTCAAC CGGCACAGTT CCTTTCCTGT GCACTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCAAGC CTCTGTTCCA 
CCTTTTCTGT CGTCTACCTC CTCTTCCACT CCACATTTTC CGCCTGTCCA CCACTCTCAG 
AGCTCTGACC TAGTGGTGGT GCCAACCGCA GCCAACCCGC CTCCAAGCAC TGTGGATGGG 
CCCACCTCCT CTTCTCAGGA GAACCGCTTT AATGGGAATG CGTCTGTCTG CCTTCCTTCT 
GAACCTCTCA CTGATTCTCT CCAGACGTCT TCGGAATCAC CAGTTGCCAA AGATGGGCAC 
CCTAAAGATT CCTCAACACA CACCAATGCC ACTGGGAAAG AAAGCCGAGA AGGTGGTGAA 
AGGCAACAAA ATTCACCAGA TGCTTTGGAA TCAGCACAGT CAGAGGAGGA GGTTGATGAG 
CTCTCCTTGA TTGACCACAG TGAAATTATG TCAAGATTGA CACTTAAGCA AGAGGGTGAC 
GATGGGCCAG ATGTGCGTGG CGGATCTGGG GATATCTTGT TGGTCCATGC AACCCAGACT 
GACAGAAAAG ATTTGGTTGT GTACTGTGAA GCCTTTTTGA CAACGTACAG GACGTTCATC 
ACCCCCGAGG AGCTCATCAA AAAGCTGCAG TACAGATATG AGAAGTTCTG CCCGTTCCCT 
GATACATTTA AGAAGCGTGT CAGCAAAAAT ACTTTCTTTG TGCTGGTGCG AGTGGTGGAT 
GAATTGTGTC TAGTGGAATT GACAGAAGAG ATTTTAAAGC TACTGATGGA GCTTGTGTTT 
CGGTTAGTGT GCAATGGGGA ACTCAGCCTT GCCCGAGTGC TGCGGAAAAA CATCCTAGAT 
AAAGTGGACC AGAAGAAGAT GCTGAAATGT GCTAACTCGG ATCAGCCCTT AGCTGCGAGA 
GGTGTGGCAG CCAGACCAGG AACCCTCCAC GATTTTCATA GCCATGAAAT AGCAGAACAG 
CTAACGCTGT TAGACGCCGA ACTCTTCTAT AAAATAGAGA TTCCAGAAGT TTTGCTTTGG 
GCAAAGGAGC AGAATGAAGA GAAGAGCCCC AACCTGACAC AGTTCACTGA GCACTTTAAT 
AACATGTCCT ACTGGGTCCG ATCGATAATA ATGCTACAGG AAAAGGCACA GGATAGGGAG 
AGGCTGCTCC TGAAATTCAT CAAGATCATG AAGCACTTGC GAAAGCTGAA TAACTTTAAC 
TCCTATCTGG CCATTCTCTC GGCTTTGGAC TCCGCACCCA TCAGAAGGCT AGAGTGGCAG 
AAGCAGACAT CAGAGGGCCT AGCTGAGTAC TGCACTTTGA TTGACAGCTC ATCCTCCTTC 
CGGGCCTATC GGGCGGCTCT ATCAGAAGTA GAACCTCCCT GTATCCCATA CCTGGGGCTG 
ATCCTGCAGG ACCTCACCTT CGTTCACCTT GGAAACCCAG ACTACATTGA TGGGAAAGTA 
AATTTCTCTA AACGCTGGCA ACAGTTTAAC ATCTTGGACA GCATGAGGCG CTTCCAACAA 
GTACATTATG AAATCCGGAG AAATGACGAT ATCGTGAACT TCTTCAACGA TTTCAGTGAT 
CACCTGGCTG AGGAGGCCCT ATGGGAACTC TCTCTTAAAA TCAAACCCCG AAACATACCA 
CGGCGAAAAA CAGACCGGGA AGAAAAAACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007660410.1
CDS	32..3604
Translation	MSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPADLTVKTPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQSDPRIQQSSAISSCYSRVYQSLANLIRWSDQVMLEGVNLEDKEMVTTVTGVIKAVLDGVKELVRLTIEKQEHPSPTSPAKLNSPACKSDGPSELPLTDREMEILNKTTGMTQSTEQLPDSMDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCNSMGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQEDLSNADQIPQQVPCNLSPLPESLGESVAPFSGSPFRLSQSSSPQAEHCSPLPSTPSLETPPALPEKKRRSAASQPSDSSSSRVSYERHPSQYDNISEDDLQNPANTQSLPFTPFAAVLPFQQGASSAPGEFVGDFAASESSGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQMPQSEHIYQQKNKYLMEVYGFNDSFSSGDSLHELAPPPALPPKQRQLASYAASSFSPVSYCVQQTKVPFTPEDTSATQGISMSISNSFLNRHSSFPVHSYKSVFRSYSQDFVPHNQASVPPFLSSTSSSTPHFPPVHHSQSSDLVVVPTAANPPPSTVDGPTSSSQENRFNGNASVCLPSEPLTDSLQTSSESPVAKDGHPKDSSTHTNATGKESREGGERQQNSPDALESAQSEEEVDELSLIDHSEIMSRLTLKQEGDDGPDVRGGSGDILLVHATQTDRKDLVVYCEAFLTTYRTFITPEELIKKLQYRYEKFCPFPDTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKMLKCANSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDDIVNFFNDFSDHLAEEALWELSLKIKPRNIPRRKTDREEKT

Target ORF information:

RefSeq Version	XM_007662220.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus Rap guanine nucleotide exchange factor 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007662220.2

ORF Insert Sequence:

ATGTCCGGCA AGATCGAGAA AGCAGATTCC CAGCGCTCTC ATCTCTCTTC GTTCACTATG 
AAGCTGATGG ACAAATTTCA TTCTCCCAAA ATAAAGAGGA CACCATCAAA GAAAGGAAAA 
CCAGCCGACC TCACTGTGAA AACCCCAGAA AAGCCAGTCA ACAAAAATCT AAGCTGGCTG 
GAAGAGAAGG AGAAGGAAGT CGTCAGTGCA TTGCGCTACT TTAAGACAAT TGTGGACAAA 
ATGGCAATTG ACAAGAAGGT GTTGGAGATG CTTCCTGGCT CTGCCAGCAA GGTGCTAGAG 
GCTATTCTAC CCCTAGTGCA GTCTGACCCT CGGATTCAAC AAAGCTCTGC AATCTCTTCC 
TGCTACAGCC GTGTATACCA GAGTCTGGCC AATCTGATTC GCTGGTCTGA TCAAGTGATG 
TTAGAAGGTG TGAACTTAGA AGATAAAGAG ATGGTGACAA CTGTCACAGG CGTCATAAAG 
GCTGTGCTTG ATGGAGTGAA GGAGCTGGTC AGACTTACTA TAGAAAAACA GGAGCATCCA 
TCCCCCACAA GCCCAGCTAA ACTCAATTCA CCAGCCTGCA AGTCTGACGG CCCCTCTGAG 
CTTCCACTGA CGGATCGAGA AATGGAGATT TTGAACAAGA CAACTGGTAT GACGCAGTCT 
ACCGAGCAAC TCCCTGATTC CATGGATGAA GAAGTTGCTC CTCCCAAGCC CCCTTTACCT 
GGTATTCGAG TGGTGGATAA TAGTCCTCCT CCAGCACTAC CACCCAAAAA GCGCCAATCA 
GCACCTTCCC CTACGAGAGT GGCAGTGGTG GCACCCATGA GTCGAGCCAC CAGTGGCTCT 
AGTTTACCTA TTGGAATTAA TAGACAGGAT TTTGATGTTG ACTGTTACGC ACAGAGGAGG 
TTGTCAGGAG GCAGCCACTC GTATGGTGGG GAATCCCCCC GCCTCTCCCC CTGCAACAGC 
ATGGGCAAGC TCAGCAAATC CGATGAGCAG CTGTCCTCCT TGGACCGGGA CAGTGGGCAG 
TGCTCCCGAA ATACAAGCTG TGAAACACTA GATCACTACG ATCCAGATTA TGAATTCCTG 
CAGGAAGACC TCTCCAATGC TGACCAGATA CCTCAACAGG TGCCCTGTAA CCTTAGCCCA 
TTGCCAGAAT CCTTGGGTGA ATCTGTTGCT CCATTTTCCG GGAGTCCTTT CAGGCTCTCC 
CAGAGCAGTT CCCCACAGGC GGAGCACTGC AGTCCTCTAC CCTCAACACC GTCTTTGGAG 
ACGCCACCGG CTCTTCCGGA GAAGAAACGG AGGAGCGCCG CCTCTCAGCC CTCGGACAGT 
TCCAGCAGTA GGGTCTCCTA TGAGAGGCAC CCTTCCCAAT ATGACAACAT TTCCGAGGAC 
GACCTGCAGA ACCCAGCCAA CACACAGTCA TTACCTTTCA CGCCGTTTGC TGCCGTTCTC 
CCCTTTCAGC AGGGGGCTTC TTCAGCACCA GGGGAATTTG TTGGGGATTT TGCTGCTTCA 
GAGTCTTCAG GTGATCCAGA AAAACCACCT CCTCTCCCAG AGAAGAAGAA CAAGCACATG 
TTGGCGTACA TGCAGCTCTT GGAGGACTAT TCAGAGCCTC AGCCCTCCAT GTTCTACCAG 
ATGCCCCAGA GCGAGCACAT TTACCAACAG AAGAACAAGT ACTTAATGGA AGTCTATGGA 
TTCAATGACT CGTTCAGTAG CGGCGACTCA CTGCATGAGT TGGCCCCTCC CCCTGCCCTT 
CCTCCCAAAC AGAGACAGCT GGCCTCCTAT GCTGCTTCTT CCTTCTCCCC TGTCTCCTAC 
TGTGTCCAGC AAACTAAAGT GCCCTTCACT CCTGAGGACA CCAGTGCCAC TCAAGGCATC 
AGTATGTCCA TCTCAAACTC CTTTCTCAAC CGGCACAGTT CCTTTCCTGT GCACTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACAACCAAGC CTCTGTTCCA 
CCTTTTCTGT CGTCTACCTC CTCTTCCACT CCACATTTTC CGCCTGTCCA CCACTCTCAG 
AGCTCTGACC TAGTGGTGGT GCCAACCGCA GCCAACCCGC CTCCAAGCAC TGTGGATGGG 
CCCACCTCCT CTTCTCAGGA GAACCGCTTT AATGGGAATG CGTCTGTCTG CCTTCCTTCT 
GAACCTCTCA CTGATTCTCT CCAGACGTCT TCGGAATCAC CAGTTGCCAA AGATGGGCAC 
CCTAAAGATT CCTCAACACA CACCAATGCC ACTGGGAAAG AAAGCCGAGA AGGTGGTGAA 
AGGCAACAAA ATTCACCAGA TGCTTTGGAA TCAGCACAGT CAGAGGAGGA GGTTGATGAG 
CTCTCCTTGA TTGACCACAG TGAAATTATG TCAAGATTGA CACTTAAGCA AGAGGGTGAC 
GATGGGCCAG ATGTGCGTGG CGGATCTGGG GATATCTTGT TGGTCCATGC AACCCAGACT 
GACAGAAAAG ATTTGGTTGT GTACTGTGAA GCCTTTTTGA CAACGTACAG GACGTTCATC 
ACCCCCGAGG AGCTCATCAA AAAGCTGCAG TACAGATATG AGAAGTTCTG CCCGTTCCCT 
GATACATTTA AGAAGCGTGT CAGCAAAAAT ACTTTCTTTG TGCTGGTGCG AGTGGTGGAT 
GAATTGTGTC TAGTGGAATT GACAGAAGAG ATTTTAAAGC TACTGATGGA GCTTGTGTTT 
CGGTTAGTGT GCAATGGGGA ACTCAGCCTT GCCCGAGTGC TGCGGAAAAA CATCCTAGAT 
AAAGTGGACC AGAAGAAGAT GCTGAAATGT GCTAACTCGG ATCAGCCCTT AGCTGCGAGA 
GGTGTGGCAG CCAGACCAGG AACCCTCCAC GATTTTCATA GCCATGAAAT AGCAGAACAG 
CTAACGCTGT TAGACGCCGA ACTCTTCTAT AAAATAGAGA TTCCAGAAGT TTTGCTTTGG 
GCAAAGGAGC AGAATGAAGA GAAGAGCCCC AACCTGACAC AGTTCACTGA GCACTTTAAT 
AACATGTCCT ACTGGGTCCG ATCGATAATA ATGCTACAGG AAAAGGCACA GGATAGGGAG 
AGGCTGCTCC TGAAATTCAT CAAGATCATG AAGCACTTGC GAAAGCTGAA TAACTTTAAC 
TCCTATCTGG CCATTCTCTC GGCTTTGGAC TCCGCACCCA TCAGAAGGCT AGAGTGGCAG 
AAGCAGACAT CAGAGGGCCT AGCTGAGTAC TGCACTTTGA TTGACAGCTC ATCCTCCTTC 
CGGGCCTATC GGGCGGCTCT ATCAGAAGTA GAACCTCCCT GTATCCCATA CCTGGGGCTG 
ATCCTGCAGG ACCTCACCTT CGTTCACCTT GGAAACCCAG ACTACATTGA TGGGAAAGTA 
AATTTCTCTA AACGCTGGCA ACAGTTTAAC ATCTTGGACA GCATGAGGCG CTTCCAACAA 
GTACATTATG AAATCCGGAG AAATGACGAT ATCGTGAACT TCTTCAACGA TTTCAGTGAT 
CACCTGGCTG AGGAGGCCCT ATGGGAACTC TCTCTTAAAA TCAAACCCCG AAACATACCA 
CGGCGAAAAA CAGACCGGGA AGAAAAAACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF1
Entrez Gene ID	100075047
Full Name	Rap guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RAPGEF1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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