NEFL cDNA ORF clone, Orcinus orca(killer whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFL
Entrez Gene ID	101277008
Full Name	neurofilament light
Gene Type	protein-coding
Organism	Orcinus orca(killer whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004270672.1	XP_004270720.1	neurofilament light polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOr150341	XM_004270672.1 Latest version!	Orcinus orca neurofilament, light polypeptide (NEFL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFL ( XM_004270672.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_004270720.1 Orcinus orca neurofilament, light polypeptide (NEFL), mRNA.

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CloneID	OOr150341
Clone ID Related Accession (Same CDS sequence)	XM_004270672.1
Accession Version	XM_004270672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofilament light polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438452.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCT TCAGCTTTGA GCCGTACTAC TCGACTTCCT ACAAACGGCG CTATGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CGGCGCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCCGGCTCCT TGTTGCCCAG TCTCGAGAAC CTCGACCTGA GCCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACCCAGGAG AAGGCGCAGC TGCAGGACCT CAACGACCGC 
TTCGCTAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGT GCTGTACGAG 
CAGGAGATCC GCGACCTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAAGGGCT GGAGGAGACT CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGTGAGGA CGCAGAGGGC CGGCTGATGG AAGCGCGCAA AGGGGCCGAC 
GAAGCGGCTC TCGCCCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGACGAAATC 
GCTTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCGCAGATCT CCGTGGAGAT GGATGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC GGAAGAGTGG 
TTCAAGAGCC GCTTCACCGT GCTGACCGAG AGCGCCGCCA AGAACACCGA TGCGGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTAA AGGCCAAGAC CCTGGAGATC 
GAAGCATGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TACAGGAGCT GGAGGACAAG 
CAGAACGCCG ACATTAGTGC TATGCAGGAC ACGATCAACA AATTGGAAAA TGAATTGAGA 
ACCACAAAGG GTGAAATGGC AAGATACCTC AAGGAATACC AAGACCTGCT CAATGTGAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA GGAGACCCGG 
CTCAGTTTCA CCAGCGTGGG CAGCATAACG AGCGGCTACT CCCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGTGG TTTACAGACC AGCTCCTACT TGATGTCTGC CCGCTCCTTC 
CCATCTTACT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AGGTGGAGGA GACCATCGAG 
CCTGCGAAAG CTGAGGAAGC CAAGGAGCAG CCCCCCTCTG AAGGAGAAGC TGAGGCGGAG 
GAGAAGAAGG AAGAGGCTGA GGCGGAGGCG GAGGCGGAGG CTGAGGCGGA GGCTGAAGCC 
GAGGCTGAAG AGGAGGGAGC CCAAGAGGAA GAAGCTGCCA AGGAAGAATC TGAGAAGGCA 
AAGGAGGAAG AAGAAGGAGG TGAAGGCAAA GAAGCAGAAG AAACCAAAGA AGCTGAGGAG 
GAGGAAAAAA AAGATGAAGG TGCTGGGGAG GAACAAGCCA CTAAGAAGAA AGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004270720.1
CDS	77..1753
Translation	MSSFSFEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLLPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRVLYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKGEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSARSFPSYYTSHVQEEQIEVEETIEPAKAEEAKEQPPSEGEAEAEEKKEEAEAEAEAEAEAEAEAEAEEEGAQEEEAAKEESEKAKEEEEGGEGKEAEETKEAEEEEKKDEGAGEEQATKKKD

Target ORF information:

RefSeq Version	XM_004270672.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofilament, light polypeptide (NEFL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004270672.1

ORF Insert Sequence:

ATGAGTTCCT TCAGCTTTGA GCCGTACTAC TCGACTTCCT ACAAACGGCG CTATGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CGGCGCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCCGGCTCCT TGTTGCCCAG TCTCGAGAAC CTCGACCTGA GCCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACCCAGGAG AAGGCGCAGC TGCAGGACCT CAACGACCGC 
TTCGCTAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGT GCTGTACGAG 
CAGGAGATCC GCGACCTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAAGGGCT GGAGGAGACT CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGTGAGGA CGCAGAGGGC CGGCTGATGG AAGCGCGCAA AGGGGCCGAC 
GAAGCGGCTC TCGCCCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGACGAAATC 
GCTTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCGCAGATCT CCGTGGAGAT GGATGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC GGAAGAGTGG 
TTCAAGAGCC GCTTCACCGT GCTGACCGAG AGCGCCGCCA AGAACACCGA TGCGGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTAA AGGCCAAGAC CCTGGAGATC 
GAAGCATGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TACAGGAGCT GGAGGACAAG 
CAGAACGCCG ACATTAGTGC TATGCAGGAC ACGATCAACA AATTGGAAAA TGAATTGAGA 
ACCACAAAGG GTGAAATGGC AAGATACCTC AAGGAATACC AAGACCTGCT CAATGTGAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA GGAGACCCGG 
CTCAGTTTCA CCAGCGTGGG CAGCATAACG AGCGGCTACT CCCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGTGG TTTACAGACC AGCTCCTACT TGATGTCTGC CCGCTCCTTC 
CCATCTTACT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AGGTGGAGGA GACCATCGAG 
CCTGCGAAAG CTGAGGAAGC CAAGGAGCAG CCCCCCTCTG AAGGAGAAGC TGAGGCGGAG 
GAGAAGAAGG AAGAGGCTGA GGCGGAGGCG GAGGCGGAGG CTGAGGCGGA GGCTGAAGCC 
GAGGCTGAAG AGGAGGGAGC CCAAGAGGAA GAAGCTGCCA AGGAAGAATC TGAGAAGGCA 
AAGGAGGAAG AAGAAGGAGG TGAAGGCAAA GAAGCAGAAG AAACCAAAGA AGCTGAGGAG 
GAGGAAAAAA AAGATGAAGG TGCTGGGGAG GAACAAGCCA CTAAGAAGAA AGATTGA