irf-1 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	irf-1
Entrez Gene ID	100135950
Full Name	interferon regulatory factor 1
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021622398.1	XP_021478073.1	interferon regulatory factor 1 isoform X1
NM_001124293.1	NP_001117765.1	interferon regulatory factor 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2).
Collet B, Hovens GC, Mazzoni D, Hirono I, Aoki T, Secombes CJ
Developmental and comparative immunology27(2)111-26(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following irf-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irf-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00831	NM_001124293.1 Latest version!	Oncorhynchus mykiss interferon regulatory factor 1 (irf-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OOf57559	XM_021622398.1 Latest version!	Oncorhynchus mykiss interferon regulatory factor 1 (irf-1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

irf-1 ( NM_001124293.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117765.1 Oncorhynchus mykiss interferon regulatory factor 1 (irf-1), mRNA.

Price & Availability↑

CloneID	OOf00831
Clone ID Related Accession (Same CDS sequence)	NM_001124293.1
Accession Version	NM_001124293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interferon regulatory factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF332147.1. ##Evidence-Data-START## Transcript exon combination :: AF332147.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041048, SAMD00041049 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGTGT CTAGGATGAG AATGAGACCT TGGTTGGAGG AAAAGATTGA GTCCAACACC 
ATCAGTGGAT TGGTGTGGGT GGACAAGGAC AATAAGATTT TCTCCATCCC ATGGAAGCAT 
GCTGCACGTC ATGGATGGGA CTTGAACAAG GACGCATGTC TGTTCAAGCA ATGGGCCATG 
CACACAGGGA AATTCATACA AGGCGAGACT AAACCGGACC CTAAGACATG GAAGGCTAAT 
TTCCGCTGTG CAATGAACTC CCTTCCTGAC ATAGAGGAAG TGAAAGACAA AAGCATCAAC 
AGAGGGAGTG GAGCGGTGCG TGTCTACAAG ATGAAGAACG TCCACTCCAA GCCAAATAAC 
AAGAGGTCAA AAGCAAACAC TGTAAAGAAA AATAACAAGG GGTCACAGAT CAAGACAGAG 
GGAATGTCGT ACAGCGAAAC CATTTGCCCC GAGGACCTCA ATACCAACAC ACACCCGCAG 
GAGGACAGTA TGACACAGGA AAGCATAGTA GACAGCACAG ACAGCCTAGA TGATTTCACA 
TTTGCTCCAG AGTGTTCCAC CAGTGTGGAA ATAGGCCCAG ACAGCACCAA CAACTTCTAC 
ACATCTTTCC AAGTGTCACC AGACCACCCC ACAGACTATG AAGACAGTCA CCAAGAAACC 
CTTATTGAGA TGACACAGCT TTGGGAGCAA GGCAGTGTAA ACGACAAGAG GTTCCTGAGC 
AATGAAGTAG ACACCTCAGA GTCCTTCGAC ACTGCAGAGT CTTACCACAG TCCAGAGAGC 
CAATGGAGTG ATAACTCAGG GGAGGAAATA GAGCTGCGGC TGTACACAGA ACTCATCTCA 
GGACTACCGA TCATTGACGA CATCCCTTAC ACCGACTACT GGTCACTGAC AACAACACAT 
CCAGTTATCC CCAACAGATC ACATGCCCAC TTCTTGAGGC CCGCTCTCTC AGACCCTGAA 
CAGAACCAAA AGACTTCATT CCCGACTTTT TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117765.1
CDS	146..1141
Translation	MPVSRMRMRPWLEEKIESNTISGLVWVDKDNKIFSIPWKHAARHGWDLNKDACLFKQWAMHTGKFIQGETKPDPKTWKANFRCAMNSLPDIEEVKDKSINRGSGAVRVYKMKNVHSKPNNKRSKANTVKKNNKGSQIKTEGMSYSETICPEDLNTNTHPQEDSMTQESIVDSTDSLDDFTFAPECSTSVEIGPDSTNNFYTSFQVSPDHPTDYEDSHQETLIEMTQLWEQGSVNDKRFLSNEVDTSESFDTAESYHSPESQWSDNSGEEIELRLYTELISGLPIIDDIPYTDYWSLTTTHPVIPNRSHAHFLRPALSDPEQNQKTSFPTFS

Target ORF information:

RefSeq Version	NM_001124293.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interferon regulatory factor 1 (irf-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124293.1

ORF Insert Sequence:

ATGCCTGTGT CTAGGATGAG AATGAGACCT TGGTTGGAGG AAAAGATTGA GTCCAACACC 
ATCAGTGGAT TGGTGTGGGT GGACAAGGAC AATAAGATTT TCTCCATCCC ATGGAAGCAT 
GCTGCACGTC ATGGATGGGA CTTGAACAAG GACGCATGTC TGTTCAAGCA ATGGGCCATG 
CACACAGGGA AATTCATACA AGGCGAGACT AAACCGGACC CTAAGACATG GAAGGCTAAT 
TTCCGCTGTG CAATGAACTC CCTTCCTGAC ATAGAGGAAG TGAAAGACAA AAGCATCAAC 
AGAGGGAGTG GAGCGGTGCG TGTCTACAAG ATGAAGAACG TCCACTCCAA GCCAAATAAC 
AAGAGGTCAA AAGCAAACAC TGTAAAGAAA AATAACAAGG GGTCACAGAT CAAGACAGAG 
GGAATGTCGT ACAGCGAAAC CATTTGCCCC GAGGACCTCA ATACCAACAC ACACCCGCAG 
GAGGACAGTA TGACACAGGA AAGCATAGTA GACAGCACAG ACAGCCTAGA TGATTTCACA 
TTTGCTCCAG AGTGTTCCAC CAGTGTGGAA ATAGGCCCAG ACAGCACCAA CAACTTCTAC 
ACATCTTTCC AAGTGTCACC AGACCACCCC ACAGACTATG AAGACAGTCA CCAAGAAACC 
CTTATTGAGA TGACACAGCT TTGGGAGCAA GGCAGTGTAA ACGACAAGAG GTTCCTGAGC 
AATGAAGTAG ACACCTCAGA GTCCTTCGAC ACTGCAGAGT CTTACCACAG TCCAGAGAGC 
CAATGGAGTG ATAACTCAGG GGAGGAAATA GAGCTGCGGC TGTACACAGA ACTCATCTCA 
GGACTACCGA TCATTGACGA CATCCCTTAC ACCGACTACT GGTCACTGAC AACAACACAT 
CCAGTTATCC CCAACAGATC ACATGCCCAC TTCTTGAGGC CCGCTCTCTC AGACCCTGAA 
CAGAACCAAA AGACTTCATT CCCGACTTTT TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irf-1 ( XM_021622398.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021478073.1 Oncorhynchus mykiss interferon regulatory factor 1 (irf-1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf57559
Clone ID Related Accession (Same CDS sequence)	XM_021622398.1
Accession Version	XM_021622398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interferon regulatory factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035088.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGT CTAGGATGAG AATGAGACCT TGGTTGGAGG GAAAGATTGA GTCCAACACC 
ATCAGTGGAT TGGTGTGGGT GGACAAGGAC AATAAGATTT TCTCCATCCC ATGGAAGCAT 
GCTGCACGTC ATGGATGGGA CTTGAACAAG GACGCATGTC TGTTCAAGCA ATGGGCCATG 
CACACAGGGA AATTCATACA AGGCGAGACT AAACCGGACC CTAAGACATG GAAGGCTAAT 
TTCCGCTGTG CAATGAACTC CCTTCCTGAC ATAGAGGAAG TGAAAGACAA AAGCATCAAC 
AGAGGGAGTG GAGCGGTGCG TGTCTACAAG ATGAAGAACA TCCACTCCAA GCCAAATAAC 
AAGAGGTCAA AAGCAAACAC TGTAAAGAAA AATAACAAGG GGTCACAGAT CAAGACAGAG 
GGAATGTCGT ACAGCGAAAC CATTTGCCCC GAGGACCTCA ATACCAACAC ACACCCGCAG 
GAGGACAGTA TGACACAGGA AAGCATAGTA GACAGCACAG ACAACCTAGA TGATTTCACA 
TTTGCTCCAG AGTGTTCCAC CAGTGTGGAA ATAGGCCCAG ACAGCACCAA CAACTTCTAC 
ACATCTTTCC AAGTGTCACC AGACCACCCC ACAGACTATG AAGACAGTCA CCAAGAAACC 
CTTATTGAGA TGACACAGCT TTGGGAGCAA GGCAGTGTAA ACGACAAGAG GTTCCTGAGC 
AATGAAGTAG ACACCTCAGA GTCCTTCGAC ACTGCAGAGT CTTACCACAG TCCAGAGAGC 
CAATGGAGTG ATAACTCAGG GGAGGAAATA GAGCTGCGGC TGTACACAGA ACTCATCTCA 
GGACTACCGA TCATTGACGA CATCCCTTAC ACCGACTACT GGTCACTGAA CAACAACACA 
TCCAGTTATC CCCAACAGAT CACATGCCCA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021478073.1
CDS	159..1094
Translation	MPVSRMRMRPWLEGKIESNTISGLVWVDKDNKIFSIPWKHAARHGWDLNKDACLFKQWAMHTGKFIQGETKPDPKTWKANFRCAMNSLPDIEEVKDKSINRGSGAVRVYKMKNIHSKPNNKRSKANTVKKNNKGSQIKTEGMSYSETICPEDLNTNTHPQEDSMTQESIVDSTDNLDDFTFAPECSTSVEIGPDSTNNFYTSFQVSPDHPTDYEDSHQETLIEMTQLWEQGSVNDKRFLSNEVDTSESFDTAESYHSPESQWSDNSGEEIELRLYTELISGLPIIDDIPYTDYWSLNNNTSSYPQQITCPL

Target ORF information:

RefSeq Version	XM_021622398.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interferon regulatory factor 1 (irf-1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021622398.1

ORF Insert Sequence:

ATGCCTGTGT CTAGGATGAG AATGAGACCT TGGTTGGAGG GAAAGATTGA GTCCAACACC 
ATCAGTGGAT TGGTGTGGGT GGACAAGGAC AATAAGATTT TCTCCATCCC ATGGAAGCAT 
GCTGCACGTC ATGGATGGGA CTTGAACAAG GACGCATGTC TGTTCAAGCA ATGGGCCATG 
CACACAGGGA AATTCATACA AGGCGAGACT AAACCGGACC CTAAGACATG GAAGGCTAAT 
TTCCGCTGTG CAATGAACTC CCTTCCTGAC ATAGAGGAAG TGAAAGACAA AAGCATCAAC 
AGAGGGAGTG GAGCGGTGCG TGTCTACAAG ATGAAGAACA TCCACTCCAA GCCAAATAAC 
AAGAGGTCAA AAGCAAACAC TGTAAAGAAA AATAACAAGG GGTCACAGAT CAAGACAGAG 
GGAATGTCGT ACAGCGAAAC CATTTGCCCC GAGGACCTCA ATACCAACAC ACACCCGCAG 
GAGGACAGTA TGACACAGGA AAGCATAGTA GACAGCACAG ACAACCTAGA TGATTTCACA 
TTTGCTCCAG AGTGTTCCAC CAGTGTGGAA ATAGGCCCAG ACAGCACCAA CAACTTCTAC 
ACATCTTTCC AAGTGTCACC AGACCACCCC ACAGACTATG AAGACAGTCA CCAAGAAACC 
CTTATTGAGA TGACACAGCT TTGGGAGCAA GGCAGTGTAA ACGACAAGAG GTTCCTGAGC 
AATGAAGTAG ACACCTCAGA GTCCTTCGAC ACTGCAGAGT CTTACCACAG TCCAGAGAGC 
CAATGGAGTG ATAACTCAGG GGAGGAAATA GAGCTGCGGC TGTACACAGA ACTCATCTCA 
GGACTACCGA TCATTGACGA CATCCCTTAC ACCGACTACT GGTCACTGAA CAACAACACA 
TCCAGTTATC CCCAACAGAT CACATGCCCA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2).
Developmental and comparative immunology27(2)111-26(2003 Feb)
Collet B,Hovens GC,Mazzoni D,Hirono I,Aoki T,Secombes CJ