smc3 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smc3
Entrez Gene ID	107390725
Full Name	structural maintenance of chromosomes 3
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015967597.1	XP_015823083.1	structural maintenance of chromosomes protein 3

Pathways from BioSystems
Gene Ontology

The following smc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo02270	XM_015967597.1 Latest version!	Nothobranchius furzeri structural maintenance of chromosomes 3 (smc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smc3 ( XM_015967597.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015823083.1 Nothobranchius furzeri structural maintenance of chromosomes 3 (smc3), mRNA.

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CloneID	ONo02270
Clone ID Related Accession (Same CDS sequence)	XM_015967597.1
Accession Version	XM_015967597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	structural maintenance of chromosomes protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443482.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCA AACAGGTTAT TATTCAGGGG TTCCGAAGTT ACAGGGATCA AACTGTCGTG 
GACCCTTTTA GTCCCAAACA TAATGTGATC GTTGGCAGAA ATGGATCAGG AAAGAGTAAC 
TTTTTTTACG CCATCCAGTT TGTTCTCAGT GATGAATTTA GCCACCTGCG GCCTGAACAG 
CGGCTGGCCC TTCTTCATGA AGGAACTGGT CCACGTGTCA TTTCAGCTTT TGTGGAGATC 
ATATTTGACA ACTCTGACAA CCGTCTGCCG ATTGACAAGG AGGAAGTCTC CCTTCGCCGT 
GTCATTGGTG CAAAGAAGGA CCAGTACTTC TTGGATAAGA AGATGGTGAC TAAGAACGAC 
GTCATGAACC TTCTGGAGAG TGCCGGCTTC TCTCGCAGTA ACCCCTATTA CATTGTGAAG 
CAGGGAAAGA TCAACCAAAT GGCCACAGCA CCTGACTCCC AGCGTCTGAA GCTCCTCAGG 
GAGGTCGCAG GAACTCGAGT GTATGACGAA CGAAAGGAGG AGAGCATTTC TCTAATGAAG 
GAGACGGAGG GGAAACGAGA AAAGATCAAC GAGCTGTTGA AGTACATCGA GGAACGTCTA 
CACACCCTGG AGGACGAGAA GGAAGAGCTG GCTCAGTATC AGAAGTGGGA CAAGATGAGA 
AGAGCTCTGG AGTACACCAT CTACAACCAG GAGCTGAACG AAACTCGAGC AAAACTGGAC 
GAGTTGTCCA GCAAGAGAGA GACATGTGGA GACAAATCCA GACAGCTGCG AGACGCTCAG 
CAAGACGCTC GAGACAAAGT GGAGGAGACG GAGCGCGTTG TGCGTGAGCT GAAGTCCAAA 
ATCTCCGCTA TGAAGGAGGA GAGAGAGCAG CTATCAGCAG AGCGCCAAGA GCAGATCAAG 
CAGAGGACCA AGCTGGAGTT GAAGGCCAAG GACCTGCAGG ATGAGCTGGC AGGCAACAGC 
GAACAGAGGA AACGACTGCT GAAGGAGCGT CAGAAACTGC TGGAGAAAAT TGAGGAGAAG 
CAAAAAGAGT TGCAGGAGAC TGAACCCAAA TTCAACATGG TGAAGGAAAA GGAGGAGCGC 
GGCATCTCCA GACTGGCTCA AGCTACACAA GAGAGGACCG ACCTGTATGC TAAGCAGGGT 
CGTGGCAGTC AGTTCACCTC CAAGGAGGAG AGGGACAAGT GGATCAAGAA GGAGCTGAAG 
TCTCTGGACC AGGCCATCAA TGACAAAAAA AGGCAGATCG CAGCCATCCA CAAAGACCTG 
GAGGACACGG AGATGAACAA GGAGAAGAAC CTTGAGCAGT ACAATAAACT TGATCAAGAT 
CTGAATGAGG TCAAAACCCG CGTCGAGGAG CTGGACAAAA AATATTACGA AGTGAAGAAC 
AAAAAAGATG AACTCCAAAG CGAAAGAAAC TACCTGTGGC GTGAGGAGAA CGCGGAGCAG 
CAGGCTTTGG CAGCCAAACG AGAGGACCTG GAAAAGAAGC AGCAGCTTCT TAGAGCGGCC 
ACCGGCAAGG CTATTCTGAA CGGTATTGAC AGCATCAACA AAGTCCTGGA GCATTTTAGG 
CGGAAGGGGA TTAACCAGCA TGTCATCAAC GGTTACCATG GCATCGTCAT GAATAACTTT 
GAGTGTGAGC CTGCCTTTTA CACGTGTGTG GAGGTGACCG CTGGAACTAG ACTGTTTTAC 
CACATTGTGG AGACGGATGA AGTGAGCACT AAAATCCTAA TGGAGTTCAA CAAGATGAAC 
TTACCTGGAG AAGTCACCTT CCTCCCTCTG AGCAAACTGG ATGTCCGAGA CACCGCCTAC 
CCAGAGACCA ACGACGCCAT CCCAATGATC AGCAAGCTGC GCTACAGCCC CAACTTTGAC 
AAGGCCTTCA AGCATGTGTT TGGGAAGACC CTCATCTGTA GAAGCATGGA GGTCTCCACT 
CAGCTGGCGA GAGCTTTCAC CATGGACTGT ATCACTCTGG AGGGTGACCA GGTGAGCCAC 
CGTGGAGCTC TGACAGGAGG CTACTACGAC ACCAGAAAGT CTCGTCTGGA GCTCCAGAAG 
GATATGAGGA AGGCTGAAGA AGAGCTGGCT GAGCTGGAGG CCAAGCTGAA TGAGAACCTG 
CGTAGAAACA TCGAACGCAT CAACAATGAG ATCGACCAGC TGATGAACCA GATGCAGCAA 
ATTGAGACGC AGCAGAGGAA GTTTAAGGCC TCCAGAGACA GCATCCTATC AGAAATGAAG 
ATGCTGAAGG AGAAGAGGCA GCAGTCGGAG AAGACGTTTA TGCCCAAGCA ACGTAGCCTC 
CAAAGCTTGG AGGCCAGCCT TCACGCAATG GAGTCCACCA GAGAGTCTCT GAAGGCTGAG 
CTGGGCACAG ATCTCCTCTC CCAGCTCAGC CTGGAGGACC AGAGGCGCGT GGACGCCCTC 
AACGATGAGA TTCGTCAGCT ACAGCAGGAC AACAGGCAGC TGTTGAACGA GAGGATCAAG 
CTGGAGGGAA TCATGACCCG AGTGGAAACA TATCTGAATG AGAACTTACG TAAACGTCTA 
GACCAAGTGG AGCAGGAGCT AAATGAGCTG CGAGAAACCG AGGGAGGCAC CGTGCTCACC 
GCTACGACAT CTGAACTGGA CGGCATCAAC AAACGTGTTA AAGACACTTT GTCTCGATCT 
GAAGATCTGG ATTCTCTTAT TGACAAGACC GAAGCGGAGA TCAAGGACCA CATCAAGAGC 
ATGGAGCGGT GGAAGAACAT CGAGAAGGAG CAGAACGACG CCATCAACCA CGACACAAAG 
GAGCTGGAGA AGATGACCAA CAGGCAGGGC ATGCTGCTGA AGAAGAAAGA GGAGTGCATG 
AAGAAAATCC GAGAGCTCGG CTCGCTGCCT CAGGAGGCCT TTGAGAAGTA CCAGACCCTC 
ACACTCAAAC AGCTGTTCAG GAAACTGGAG CAGTGCAACA CGGAGCTGAA GAAGTACAGC 
CACGTCAACA AGAAGGCTCT GGACCAGTTT GTCAACTTCT CAGAGCAGAA GGAGAAGCTG 
ATCAAGCGTC AGGATGAGCT CGACCGCGGC TACAAATCCA TCATGGAGCT CATGAACGTT 
CTGGAGCTGC GCAAGTACGA GGCCATCCAG CTCACCTTCA AACAGGTGTC GAAGAACTTC 
AGCGAGGTTT TCCAGAAGCT GGTGCCAGGT GGCAAAGCGA CGCTGGTGAT GAAGAAGGGT 
GACGCTGAAG GCAGCCAGTC CCAGGATGAA GGAGAGGGGG GTGCAGACAG TGAGAGGGGG 
TCTGGCTCTC AGAGCAGCGT TCCCTCAGTG GACCAGTTCA CCGGGGTCGG AATCAGGGTG 
TCGTTCACCG GGAAGCAGGG CGAGATGAGG GAGATGCAGC AGCTGTCGGG GGGTCAGAAG 
TCTCTGGTGG CTCTGGCGCT CATTTTTGCC ATTCAAAAGT GTGACCCCGC CCCTTTCTAC 
CTGTTTGATG AGATCGATCA GGCCCTTGAC GCTCAGCACA GAAAGGCAGT GTCAGATATG 
ATCGTTGAAC TGGCGGGCCA CGCCCAGTTC ATCACCACCA CCTTCAGGCC CGAGCTGCTG 
GAGTCAGCCG ACAAGTTCTA TGGCGTCAAG TTCAGGAACA AGGTGAGTCA CATCGATGTG 
ATCACAGCCG AGCAGGCCAA AGACTTTGTG GAGGATGACA CCACCCATGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015823083.1
CDS	180..3833
Translation	MYIKQVIIQGFRSYRDQTVVDPFSPKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEDEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSSKRETCGDKSRQLRDAQQDARDKVEETERVVRELKSKISAMKEEREQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELQETEPKFNMVKEKEERGISRLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDLEDTEMNKEKNLEQYNKLDQDLNEVKTRVEELDKKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDLEKKQQLLRAATGKAILNGIDSINKVLEHFRRKGINQHVINGYHGIVMNNFECEPAFYTCVEVTAGTRLFYHIVETDEVSTKILMEFNKMNLPGEVTFLPLSKLDVRDTAYPETNDAIPMISKLRYSPNFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDMRKAEEELAELEAKLNENLRRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQRRVDALNDEIRQLQQDNRQLLNERIKLEGIMTRVETYLNENLRKRLDQVEQELNELRETEGGTVLTATTSELDGINKRVKDTLSRSEDLDSLIDKTEAEIKDHIKSMERWKNIEKEQNDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLTLKQLFRKLEQCNTELKKYSHVNKKALDQFVNFSEQKEKLIKRQDELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPGGKATLVMKKGDAEGSQSQDEGEGGADSERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQKSLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIVELAGHAQFITTTFRPELLESADKFYGVKFRNKVSHIDVITAEQAKDFVEDDTTHG

Target ORF information:

RefSeq Version	XM_015967597.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri structural maintenance of chromosomes 3 (smc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015967597.1

ORF Insert Sequence:

ATGTACATCA AACAGGTTAT TATTCAGGGG TTCCGAAGTT ACAGGGATCA AACTGTCGTG 
GACCCTTTTA GTCCCAAACA TAATGTGATC GTTGGCAGAA ATGGATCAGG AAAGAGTAAC 
TTTTTTTACG CCATCCAGTT TGTTCTCAGT GATGAATTTA GCCACCTGCG GCCTGAACAG 
CGGCTGGCCC TTCTTCATGA AGGAACTGGT CCACGTGTCA TTTCAGCTTT TGTGGAGATC 
ATATTTGACA ACTCTGACAA CCGTCTGCCG ATTGACAAGG AGGAAGTCTC CCTTCGCCGT 
GTCATTGGTG CAAAGAAGGA CCAGTACTTC TTGGATAAGA AGATGGTGAC TAAGAACGAC 
GTCATGAACC TTCTGGAGAG TGCCGGCTTC TCTCGCAGTA ACCCCTATTA CATTGTGAAG 
CAGGGAAAGA TCAACCAAAT GGCCACAGCA CCTGACTCCC AGCGTCTGAA GCTCCTCAGG 
GAGGTCGCAG GAACTCGAGT GTATGACGAA CGAAAGGAGG AGAGCATTTC TCTAATGAAG 
GAGACGGAGG GGAAACGAGA AAAGATCAAC GAGCTGTTGA AGTACATCGA GGAACGTCTA 
CACACCCTGG AGGACGAGAA GGAAGAGCTG GCTCAGTATC AGAAGTGGGA CAAGATGAGA 
AGAGCTCTGG AGTACACCAT CTACAACCAG GAGCTGAACG AAACTCGAGC AAAACTGGAC 
GAGTTGTCCA GCAAGAGAGA GACATGTGGA GACAAATCCA GACAGCTGCG AGACGCTCAG 
CAAGACGCTC GAGACAAAGT GGAGGAGACG GAGCGCGTTG TGCGTGAGCT GAAGTCCAAA 
ATCTCCGCTA TGAAGGAGGA GAGAGAGCAG CTATCAGCAG AGCGCCAAGA GCAGATCAAG 
CAGAGGACCA AGCTGGAGTT GAAGGCCAAG GACCTGCAGG ATGAGCTGGC AGGCAACAGC 
GAACAGAGGA AACGACTGCT GAAGGAGCGT CAGAAACTGC TGGAGAAAAT TGAGGAGAAG 
CAAAAAGAGT TGCAGGAGAC TGAACCCAAA TTCAACATGG TGAAGGAAAA GGAGGAGCGC 
GGCATCTCCA GACTGGCTCA AGCTACACAA GAGAGGACCG ACCTGTATGC TAAGCAGGGT 
CGTGGCAGTC AGTTCACCTC CAAGGAGGAG AGGGACAAGT GGATCAAGAA GGAGCTGAAG 
TCTCTGGACC AGGCCATCAA TGACAAAAAA AGGCAGATCG CAGCCATCCA CAAAGACCTG 
GAGGACACGG AGATGAACAA GGAGAAGAAC CTTGAGCAGT ACAATAAACT TGATCAAGAT 
CTGAATGAGG TCAAAACCCG CGTCGAGGAG CTGGACAAAA AATATTACGA AGTGAAGAAC 
AAAAAAGATG AACTCCAAAG CGAAAGAAAC TACCTGTGGC GTGAGGAGAA CGCGGAGCAG 
CAGGCTTTGG CAGCCAAACG AGAGGACCTG GAAAAGAAGC AGCAGCTTCT TAGAGCGGCC 
ACCGGCAAGG CTATTCTGAA CGGTATTGAC AGCATCAACA AAGTCCTGGA GCATTTTAGG 
CGGAAGGGGA TTAACCAGCA TGTCATCAAC GGTTACCATG GCATCGTCAT GAATAACTTT 
GAGTGTGAGC CTGCCTTTTA CACGTGTGTG GAGGTGACCG CTGGAACTAG ACTGTTTTAC 
CACATTGTGG AGACGGATGA AGTGAGCACT AAAATCCTAA TGGAGTTCAA CAAGATGAAC 
TTACCTGGAG AAGTCACCTT CCTCCCTCTG AGCAAACTGG ATGTCCGAGA CACCGCCTAC 
CCAGAGACCA ACGACGCCAT CCCAATGATC AGCAAGCTGC GCTACAGCCC CAACTTTGAC 
AAGGCCTTCA AGCATGTGTT TGGGAAGACC CTCATCTGTA GAAGCATGGA GGTCTCCACT 
CAGCTGGCGA GAGCTTTCAC CATGGACTGT ATCACTCTGG AGGGTGACCA GGTGAGCCAC 
CGTGGAGCTC TGACAGGAGG CTACTACGAC ACCAGAAAGT CTCGTCTGGA GCTCCAGAAG 
GATATGAGGA AGGCTGAAGA AGAGCTGGCT GAGCTGGAGG CCAAGCTGAA TGAGAACCTG 
CGTAGAAACA TCGAACGCAT CAACAATGAG ATCGACCAGC TGATGAACCA GATGCAGCAA 
ATTGAGACGC AGCAGAGGAA GTTTAAGGCC TCCAGAGACA GCATCCTATC AGAAATGAAG 
ATGCTGAAGG AGAAGAGGCA GCAGTCGGAG AAGACGTTTA TGCCCAAGCA ACGTAGCCTC 
CAAAGCTTGG AGGCCAGCCT TCACGCAATG GAGTCCACCA GAGAGTCTCT GAAGGCTGAG 
CTGGGCACAG ATCTCCTCTC CCAGCTCAGC CTGGAGGACC AGAGGCGCGT GGACGCCCTC 
AACGATGAGA TTCGTCAGCT ACAGCAGGAC AACAGGCAGC TGTTGAACGA GAGGATCAAG 
CTGGAGGGAA TCATGACCCG AGTGGAAACA TATCTGAATG AGAACTTACG TAAACGTCTA 
GACCAAGTGG AGCAGGAGCT AAATGAGCTG CGAGAAACCG AGGGAGGCAC CGTGCTCACC 
GCTACGACAT CTGAACTGGA CGGCATCAAC AAACGTGTTA AAGACACTTT GTCTCGATCT 
GAAGATCTGG ATTCTCTTAT TGACAAGACC GAAGCGGAGA TCAAGGACCA CATCAAGAGC 
ATGGAGCGGT GGAAGAACAT CGAGAAGGAG CAGAACGACG CCATCAACCA CGACACAAAG 
GAGCTGGAGA AGATGACCAA CAGGCAGGGC ATGCTGCTGA AGAAGAAAGA GGAGTGCATG 
AAGAAAATCC GAGAGCTCGG CTCGCTGCCT CAGGAGGCCT TTGAGAAGTA CCAGACCCTC 
ACACTCAAAC AGCTGTTCAG GAAACTGGAG CAGTGCAACA CGGAGCTGAA GAAGTACAGC 
CACGTCAACA AGAAGGCTCT GGACCAGTTT GTCAACTTCT CAGAGCAGAA GGAGAAGCTG 
ATCAAGCGTC AGGATGAGCT CGACCGCGGC TACAAATCCA TCATGGAGCT CATGAACGTT 
CTGGAGCTGC GCAAGTACGA GGCCATCCAG CTCACCTTCA AACAGGTGTC GAAGAACTTC 
AGCGAGGTTT TCCAGAAGCT GGTGCCAGGT GGCAAAGCGA CGCTGGTGAT GAAGAAGGGT 
GACGCTGAAG GCAGCCAGTC CCAGGATGAA GGAGAGGGGG GTGCAGACAG TGAGAGGGGG 
TCTGGCTCTC AGAGCAGCGT TCCCTCAGTG GACCAGTTCA CCGGGGTCGG AATCAGGGTG 
TCGTTCACCG GGAAGCAGGG CGAGATGAGG GAGATGCAGC AGCTGTCGGG GGGTCAGAAG 
TCTCTGGTGG CTCTGGCGCT CATTTTTGCC ATTCAAAAGT GTGACCCCGC CCCTTTCTAC 
CTGTTTGATG AGATCGATCA GGCCCTTGAC GCTCAGCACA GAAAGGCAGT GTCAGATATG 
ATCGTTGAAC TGGCGGGCCA CGCCCAGTTC ATCACCACCA CCTTCAGGCC CGAGCTGCTG 
GAGTCAGCCG ACAAGTTCTA TGGCGTCAAG TTCAGGAACA AGGTGAGTCA CATCGATGTG 
ATCACAGCCG AGCAGGCCAA AGACTTTGTG GAGGATGACA CCACCCATGG TTAA