DGCR8 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGCR8
Entrez Gene ID	104412757
Full Name	DGCR8, microprocessor complex subunit
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010023562.1	XP_010021864.1	LOW QUALITY PROTEIN: microprocessor complex subunit DGCR8

Pathways from BioSystems
Gene Ontology

The following DGCR8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGCR8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe111126	XM_010023562.1 Latest version!	Nestor notabilis DGCR8 microprocessor complex subunit (DGCR8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGCR8 ( XM_010023562.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010021864.1 Nestor notabilis DGCR8 microprocessor complex subunit (DGCR8), mRNA.

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CloneID	ONe111126
Clone ID Related Accession (Same CDS sequence)	XM_010023562.1
Accession Version	XM_010023562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	LOW QUALITY PROTEIN: microprocessor complex subunit DGCR8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009914246.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGAAAT ATGAAAATGT ACCACCCCTC CCTAAAGAAC CTGCAAGAGA AATGAATGTG 
GAGAATCACA CTTGTCCCCC ACCTCTGCCG CCTAACGAAC AGCCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGACGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCAGAT 
ACCCCTGCTG GGGACACGCA CAGTATCTGC AGAACACAGC TTCTCACAAA GGGATCTGCC 
TGCTACAAAA GTCGTCTTAT AGTAGACCCT AACAATAGTG ACCAAAGCCC AAGAACTGCT 
CGTCATGCAC CGTCAGTTCG AAAGTTCACC CCAGATCTTA AGTTACTTAA AGATGTAAAA 
ATTAGTGTTA GCTTTACAGA AAGCTGCAAA AGCAAAGATA GGAAAGTTCT GTACACTGGA 
GTTGAGCAGG ATTATAAGGC AGATACAGAT TTTGGTATTA ATAATGTCAA CGGGGATTTG 
CATGTTTGTC CTTTCGGTGG TAGTAATGGT AAAGCTGTAG GAACAGGGGG TGAAAATGAT 
GACAAGAAGG ATGATGAAAA TGATATTGAT CAGGAAAAGA GAGTAGAATA TGCAGTTCTG 
GATGAGCTAG AAGATTTTAC CGACAATTTG ATGGAAATAG ATGAAGAAGG AGGAGGGTTC 
ACATCTAAAG CAATCGTTCA AAGAGATAAA GTGGATGAAG AAACCTTGAA TTACTCATAC 
GAGGATGACT TTGATAATGA TGTGGATGCT CTGCTGGAAG AAGGTCTTCG AGCACCCAAA 
ACAAGGAGAC TAGAAAATGA GAAATATGCT GGTGAAAGTG ATCACCAGTC TGATGGAGAA 
ACAAGTGTGC AGCCAATGAT GACCAAAATT AAAACTGTAC TTAAAAGTCG TGGCCGCCCT 
CCTACAGAAC CTTTGCCAGA TGGATGGATC ATGACATTCN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNA TTCCTGTATA TCTGCACAGG GAATCTAGAG TTGTTACCTG GTCTAGACCT 
TACTTCTTGG GAACAGGAAG CATAAGGAAA CATGATCCTC CCCTTACTAG CATTCCCTGC 
TTTCATTACA AAAAAATGAA GGAAAATGAG GAAAGGGAAC AAAACAATGA CATAACCCCA 
AATGGGGAAG TATCACCTAT AAAGCATCTA GATAAATCTT CAGAACTGGA CTGCCAAACA 
GAAGAACCAG ATTCCACTGC TGCTGACTCT GGGCCTTTAG ATGAGAAAGA CTCCACAGGG 
GGAGATGCAG CACAAGGAGC CTTAGGACAA GTTAAGGCCA AAGTTGAAGT ATGTAAAGAT 
GAATCTGTAG ATCTGGAAGA TTTTAGACGC TATCTTGAAA AGCGTTTTGA CTTTGAACAA 
GTTACTGTAA AAAAGTTCAG AACCTGGGCT GAGAGACGGC AATTCAACCG TGAGATGAAG 
CGGAAACAGG CAGAATCTGA GCGGCCCATT CTGCCTGCCA ACCAGAAACT CATTACCTTG 
TCTGTGCAAG ATGCACCTAC AAAGAAAGAA TTTGTCATTA ATCCCAATGG GAAATCTGAA 
GTTTGCATAC TGCACGAATA TATGCAACGA GTCCTAAAGG TTCGCCCTGT TTACAATTTC 
TTTGAATGTG AGAACCCAAG TGAACCTTTT GGAGCCTCAG TGATTATTGA TGGAGTTACT 
TATGGGGCAG GAACTGCCAG CAGCAAAAAA CTTGCCAAGA ATAAAGCTGC TCGAGCTACA 
CTGGAAATCC TTATTCCTGA CTTTGTTAAA CAGACCTCTG AGGAGAAGCC CAAAGACAGT 
GAAGAACTTG AGTATTTTAA CCATATCAGT ATTGAGGACT CACGGGTATA TGAACTCACC 
AGTAAAGCTG GACTGTTGTC TCCATATCAG ATTCTCCATG AGTGCCTTAA AAGAAACCAC 
GGAATGGGTG ACACAACCAT AAAGTTTGAA GTGATTCCTG GTAAAAATCA GAAGAGTGAA 
TATGTCATGA CTTGTGGCAA GCACACGGTG CGAGGCTGGT GCAAAAACAA GAGAGTGGGG 
AAACAACTGG CTTCTCAGAA AATCCTACAG CTACTGCATC CACACGTGAA GAATTGGGGC 
TCTCTCTTGC GTATGTATGG GAGAGAGAAC AGCAAGATGG TTAAACAGGA AACCTCTGAT 
AAAAGTGTGA TAGAGCTTCA GCAGTATGCC AAAAAGAACA AGCCAAATCT GCACATCCTG 
AACAAGTTGC AGGAAGAAAT GAAAAAACTG GCACAAGAAA GAGAGGAAAC CCGAAAGAAA 
CCCAAGATGA CAATAGTGGA GTCGGCTCAG CCTGCTAGTG AACCTCTCTG TACTGTTGAT 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010021864.1
CDS	1..2346
Translation	MEKYENVPPLPKEPAREMNVENHTCPPPLPPNEQPPPPPLQTSSDAEVMDVGSGGDGQSDTPAGDTHSICRTQLLTKGSACYKSRLIVDPNNSDQSPRTARHAPSVRKFTPDLKLLKDVKISVSFTESCKSKDRKVLYTGVEQDYKADTDFGINNVNGDLHVCPFGGSNGKAVGTGGENDDKKDDENDIDQEKRVEYAVLDELEDFTDNLMEIDEEGGGFTSKAIVQRDKVDEETLNYSYEDDFDNDVDALLEEGLRAPKTRRLENEKYAGESDHQSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFnullnullnullnullnullnullnullnullnullnullIPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLTSIPCFHYKKMKENEEREQNNDITPNGEVSPIKHLDKSSELDCQTEEPDSTAADSGPLDEKDSTGGDAAQGALGQVKAKVEVCKDESVDLEDFRRYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVIIDGVTYGAGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTTIKFEVIPGKNQKSEYVMTCGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRENSKMVKQETSDKSVIELQQYAKKNKPNLHILNKLQEEMKKLAQEREETRKKPKMTIVESAQPASEPLCTVDV

Target ORF information:

RefSeq Version	XM_010023562.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis DGCR8 microprocessor complex subunit (DGCR8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010023562.1

ORF Insert Sequence:

ATGGAGAAAT ATGAAAATGT ACCACCCCTC CCTAAAGAAC CTGCAAGAGA AATGAATGTG 
GAGAATCACA CTTGTCCCCC ACCTCTGCCG CCTAACGAAC AGCCTCCACC ACCTCCCCTG 
CAAACGTCCA GTGACGCAGA GGTAATGGAC GTTGGCTCTG GTGGTGATGG ACAGTCAGAT 
ACCCCTGCTG GGGACACGCA CAGTATCTGC AGAACACAGC TTCTCACAAA GGGATCTGCC 
TGCTACAAAA GTCGTCTTAT AGTAGACCCT AACAATAGTG ACCAAAGCCC AAGAACTGCT 
CGTCATGCAC CGTCAGTTCG AAAGTTCACC CCAGATCTTA AGTTACTTAA AGATGTAAAA 
ATTAGTGTTA GCTTTACAGA AAGCTGCAAA AGCAAAGATA GGAAAGTTCT GTACACTGGA 
GTTGAGCAGG ATTATAAGGC AGATACAGAT TTTGGTATTA ATAATGTCAA CGGGGATTTG 
CATGTTTGTC CTTTCGGTGG TAGTAATGGT AAAGCTGTAG GAACAGGGGG TGAAAATGAT 
GACAAGAAGG ATGATGAAAA TGATATTGAT CAGGAAAAGA GAGTAGAATA TGCAGTTCTG 
GATGAGCTAG AAGATTTTAC CGACAATTTG ATGGAAATAG ATGAAGAAGG AGGAGGGTTC 
ACATCTAAAG CAATCGTTCA AAGAGATAAA GTGGATGAAG AAACCTTGAA TTACTCATAC 
GAGGATGACT TTGATAATGA TGTGGATGCT CTGCTGGAAG AAGGTCTTCG AGCACCCAAA 
ACAAGGAGAC TAGAAAATGA GAAATATGCT GGTGAAAGTG ATCACCAGTC TGATGGAGAA 
ACAAGTGTGC AGCCAATGAT GACCAAAATT AAAACTGTAC TTAAAAGTCG TGGCCGCCCT 
CCTACAGAAC CTTTGCCAGA TGGATGGATC ATGACATTCN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNA TTCCTGTATA TCTGCACAGG GAATCTAGAG TTGTTACCTG GTCTAGACCT 
TACTTCTTGG GAACAGGAAG CATAAGGAAA CATGATCCTC CCCTTACTAG CATTCCCTGC 
TTTCATTACA AAAAAATGAA GGAAAATGAG GAAAGGGAAC AAAACAATGA CATAACCCCA 
AATGGGGAAG TATCACCTAT AAAGCATCTA GATAAATCTT CAGAACTGGA CTGCCAAACA 
GAAGAACCAG ATTCCACTGC TGCTGACTCT GGGCCTTTAG ATGAGAAAGA CTCCACAGGG 
GGAGATGCAG CACAAGGAGC CTTAGGACAA GTTAAGGCCA AAGTTGAAGT ATGTAAAGAT 
GAATCTGTAG ATCTGGAAGA TTTTAGACGC TATCTTGAAA AGCGTTTTGA CTTTGAACAA 
GTTACTGTAA AAAAGTTCAG AACCTGGGCT GAGAGACGGC AATTCAACCG TGAGATGAAG 
CGGAAACAGG CAGAATCTGA GCGGCCCATT CTGCCTGCCA ACCAGAAACT CATTACCTTG 
TCTGTGCAAG ATGCACCTAC AAAGAAAGAA TTTGTCATTA ATCCCAATGG GAAATCTGAA 
GTTTGCATAC TGCACGAATA TATGCAACGA GTCCTAAAGG TTCGCCCTGT TTACAATTTC 
TTTGAATGTG AGAACCCAAG TGAACCTTTT GGAGCCTCAG TGATTATTGA TGGAGTTACT 
TATGGGGCAG GAACTGCCAG CAGCAAAAAA CTTGCCAAGA ATAAAGCTGC TCGAGCTACA 
CTGGAAATCC TTATTCCTGA CTTTGTTAAA CAGACCTCTG AGGAGAAGCC CAAAGACAGT 
GAAGAACTTG AGTATTTTAA CCATATCAGT ATTGAGGACT CACGGGTATA TGAACTCACC 
AGTAAAGCTG GACTGTTGTC TCCATATCAG ATTCTCCATG AGTGCCTTAA AAGAAACCAC 
GGAATGGGTG ACACAACCAT AAAGTTTGAA GTGATTCCTG GTAAAAATCA GAAGAGTGAA 
TATGTCATGA CTTGTGGCAA GCACACGGTG CGAGGCTGGT GCAAAAACAA GAGAGTGGGG 
AAACAACTGG CTTCTCAGAA AATCCTACAG CTACTGCATC CACACGTGAA GAATTGGGGC 
TCTCTCTTGC GTATGTATGG GAGAGAGAAC AGCAAGATGG TTAAACAGGA AACCTCTGAT 
AAAAGTGTGA TAGAGCTTCA GCAGTATGCC AAAAAGAACA AGCCAAATCT GCACATCCTG 
AACAAGTTGC AGGAAGAAAT GAAAAAACTG GCACAAGAAA GAGAGGAAAC CCGAAAGAAA 
CCCAAGATGA CAATAGTGGA GTCGGCTCAG CCTGCTAGTG AACCTCTCTG TACTGTTGAT 
GTCTAA