EEF1A1 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF1A1
Entrez Gene ID	104400561
Full Name	eukaryotic translation elongation factor 1 alpha 1
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010015523.1	XP_010013825.1	elongation factor 1-alpha 1

Pathways from BioSystems
Gene Ontology

The following EEF1A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF1A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe114746	XM_010015523.1 Latest version!	Nestor notabilis eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF1A1 ( XM_010015523.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010013825.1 Nestor notabilis eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), mRNA.

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CloneID	ONe114746
Clone ID Related Accession (Same CDS sequence)	XM_010015523.1
Accession Version	XM_010015523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	elongation factor 1-alpha 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009930690.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAGG AGAAGACCCA CATCAACATC GTCGTCATCG GCCACGTCGA TTCTGGCAAA 
TCCACCACCA CCGGCCACCT CATCTACAAA TGCGGTGGTA TCGACAAGAG GACCATCGAG 
AAGTTCGAGA AGGAAGCTGC TGAGATGGGC AAAGGTTCCT TCAAATATGC CTGGGTCTTG 
GACAAGCTGA AGGCTGAGCG TGAGCGTGGT ATTACTATTG ATATTTCCCT GTGGAAATTC 
GAAACAAGCA AATACTACGT CACCATCATC GATGCTCCTG GACACAGAGA CTTCATCAAG 
AACATGATTA CTGGAACTTC TCAGGCTGAT TGTGCTGTCC TGATCGTTGC TGCTGGTGTT 
GGTGAGTTCG AGGCTGGTAT TTCCAAGAAC GGGCAGACCC GTGAGCATGC CCTTCTGGCT 
TACACCCTGG GTGTAAAACA GCTGATTGTT GGTGTCAACA AGATGGATTC CACCGAGCCA 
CCTTACAGCC AGAAGAGATA TGAAGAGATT GTCAAAGAAG TCAGCACTTA CATCAAGAAG 
ATTGGCTACA ACCCAGACAC TGTAGCTTTT GTGCCAATTT CTGGTTGGAA CGGAGACAAC 
ATGTTGGAGC CTAGCTCTAA CATGCCCTGG TTCAAGGGAT GGAAGGTTAC CCGGAAGGAT 
GGCAATGCCA GCGGAACCAC CCTCCTGGAA GCCTTGGACT GCATCTTGCC ACCAACTCGT 
CCAACTGACA AACCTCTGCG TCTGCCTCTT CAAGATGTCT ACAAAATTGG CGGCATTGGT 
ACTGTACCAG TTGGCCGTGT GGAAACTGGT GTTCTGAAGC CAGGCATGGT GGTTACATTT 
GCCCCCGTCA ACGTTACAAC TGAAGTAAAA TCTGTTGAGA TGCACCATGA AGCCTTGAGT 
GAAGCTCTGC CTGGTGACAA CGTTGGCTTC AATGTTAAGA ACGTGTCTGT GAAAGATGTT 
CGCCGTGGCA ATGTTGCTGG TGACAGCAAG AACGATCCTC CAATGGAAGC TGCTGGCTTC 
ACTGCACAGG TTATCATCCT GAACCACCCT GGCCAAATTA GTGCTGGTTA CGCCCCTGTG 
CTGGATTGCC ATACTGCTCA CATTGCATGC AAATTTGCTG AGCTCAAAGA GAAGATTGAT 
CGTCGTTCCG GCAAGAAGCT GGAGGATGGC CCCAAATTCC TGAAGTCTGG AGATGCTGCC 
ATCGTTGATA TGATCCCTGG CAAACCCATG TGTGTTGAGA GCTTCTCTGA TTACCCTCCT 
CTCGGTCGTT TTGCTGTGCG TGACATGAGA CAGACGGTTG CTGTTGGTGT CATCAAGGCA 
GTTGACAAGA AGGCCGGAGG AGCTGGCAAG GTCACAAAGT CTGCCCAGAA GGCCCAGAAA 
GCTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010013825.1
CDS	93..1481
Translation	MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSSNMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMIPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAGGAGKVTKSAQKAQKAK

Target ORF information:

RefSeq Version	XM_010015523.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010015523.1

ORF Insert Sequence:

ATGGGAAAGG AGAAGACCCA CATCAACATC GTCGTCATCG GCCACGTCGA TTCTGGCAAA 
TCCACCACCA CCGGCCACCT CATCTACAAA TGCGGTGGTA TCGACAAGAG GACCATCGAG 
AAGTTCGAGA AGGAAGCTGC TGAGATGGGC AAAGGTTCCT TCAAATATGC CTGGGTCTTG 
GACAAGCTGA AGGCTGAGCG TGAGCGTGGT ATTACTATTG ATATTTCCCT GTGGAAATTC 
GAAACAAGCA AATACTACGT CACCATCATC GATGCTCCTG GACACAGAGA CTTCATCAAG 
AACATGATTA CTGGAACTTC TCAGGCTGAT TGTGCTGTCC TGATCGTTGC TGCTGGTGTT 
GGTGAGTTCG AGGCTGGTAT TTCCAAGAAC GGGCAGACCC GTGAGCATGC CCTTCTGGCT 
TACACCCTGG GTGTAAAACA GCTGATTGTT GGTGTCAACA AGATGGATTC CACCGAGCCA 
CCTTACAGCC AGAAGAGATA TGAAGAGATT GTCAAAGAAG TCAGCACTTA CATCAAGAAG 
ATTGGCTACA ACCCAGACAC TGTAGCTTTT GTGCCAATTT CTGGTTGGAA CGGAGACAAC 
ATGTTGGAGC CTAGCTCTAA CATGCCCTGG TTCAAGGGAT GGAAGGTTAC CCGGAAGGAT 
GGCAATGCCA GCGGAACCAC CCTCCTGGAA GCCTTGGACT GCATCTTGCC ACCAACTCGT 
CCAACTGACA AACCTCTGCG TCTGCCTCTT CAAGATGTCT ACAAAATTGG CGGCATTGGT 
ACTGTACCAG TTGGCCGTGT GGAAACTGGT GTTCTGAAGC CAGGCATGGT GGTTACATTT 
GCCCCCGTCA ACGTTACAAC TGAAGTAAAA TCTGTTGAGA TGCACCATGA AGCCTTGAGT 
GAAGCTCTGC CTGGTGACAA CGTTGGCTTC AATGTTAAGA ACGTGTCTGT GAAAGATGTT 
CGCCGTGGCA ATGTTGCTGG TGACAGCAAG AACGATCCTC CAATGGAAGC TGCTGGCTTC 
ACTGCACAGG TTATCATCCT GAACCACCCT GGCCAAATTA GTGCTGGTTA CGCCCCTGTG 
CTGGATTGCC ATACTGCTCA CATTGCATGC AAATTTGCTG AGCTCAAAGA GAAGATTGAT 
CGTCGTTCCG GCAAGAAGCT GGAGGATGGC CCCAAATTCC TGAAGTCTGG AGATGCTGCC 
ATCGTTGATA TGATCCCTGG CAAACCCATG TGTGTTGAGA GCTTCTCTGA TTACCCTCCT 
CTCGGTCGTT TTGCTGTGCG TGACATGAGA CAGACGGTTG CTGTTGGTGT CATCAAGGCA 
GTTGACAAGA AGGCCGGAGG AGCTGGCAAG GTCACAAAGT CTGCCCAGAA GGCCCAGAAA 
GCTAAATGA