Cpn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpn1
Entrez Gene ID	93721
Full Name	carboxypeptidase N, polypeptide 1
Synonyms	0610011F20Rik,CPN
General protein information	Preferred Names carboxypeptidase N, polypeptide 1 Names carboxypeptidase N catalytic chain anaphylatoxin inactivator carboxypeptidase N small subunit carboxypeptidase N, polypeptide 1, 50kD kininase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C3\|19 36.68 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_030703.2	NP_109628.1	carboxypeptidase N catalytic chain precursor
XM_011247407.1	XP_011245709.1	carboxypeptidase N catalytic chain isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CPN1	NP_001299.1
Bos taurus (cattle)	CPN1	NP_001069548.1
Rattus norvegicus (Norway rat)	Cpn1	NP_445978.1
Danio rerio (zebrafish)	cpn1	NP_997935.1
Canis lupus familiaris (dog)	CPN1	XP_534989.3
Mus musculus (house mouse)	Cpn1	NP_109628.1
Gallus gallus (chicken)	CPN1	NP_001264183.1
Xenopus tropicalis (tropical clawed frog)	cpn1	NP_001017266.1

Gene Ontology
Bibliography

Related articles in PubMed

Ctip1 Regulates the Balance between Specification of Distinct Projection Neuron Subtypes in Deep Cortical Layers.
Woodworth MB, Greig LC, Liu KX, Ippolito GC, Tucker HO, Macklis JD
Cell reports15(5)999-1012(2016 05)

Ctip1 Controls Acquisition of Sensory Area Identity and Establishment of Sensory Input Fields in the Developing Neocortex.
Greig LC, Woodworth MB, Greppi C, Macklis JD
Neuron90(2)261-77(2016 04)

Carboxypeptidase N-deficient mice present with polymorphic disease phenotypes on induction of experimental autoimmune encephalomyelitis.
Hu X, Wetsel RA, Ramos TN, Mueller-Ortiz SL, Schoeb TR, Barnum SR
Immunobiology219(2)104-8(2014 Feb)

Deletion of carboxypeptidase N delays onset of experimental cerebral malaria.
Darley MM, Ramos TN, Wetsel RA, Barnum SR
Parasite immunology34(8-9)444-7(2012 Aug-Sep)

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Tamplin OJ, Cox BJ, Rossant J
Developmental biology360(2)415-25(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

plays a role in experimental autoimmune encephalomyelitis development and progression
Title: Carboxypeptidase N-deficient mice present with polymorphic disease phenotypes on induction of experimental autoimmune encephalomyelitis.

These results indicate that carboxypeptidases contribute to the pathogenesis of experimental cerebral malaria.
Title: Deletion of carboxypeptidase N delays onset of experimental cerebral malaria.

we report the generation of a mouse with complete CPN deficiency by targeted disruption of the CPN1 gene
Title: Targeted disruption of the gene encoding the murine small subunit of carboxypeptidase N (CPN1) causes susceptibility to C5a anaphylatoxin-mediated shock.

carboxypeptidase N regulates the biologic activity of SDF-1alpha by reducing the chemokine-specific activity
Title: Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1alpha in the circulation.

The following Cpn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08390	NM_030703.2 Latest version!	Mus musculus carboxypeptidase N, polypeptide 1 (Cpn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OMu71891	XM_011247407.1 Latest version!	Mus musculus carboxypeptidase N, polypeptide 1 (Cpn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpn1 ( NM_030703.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109628.1 Mus musculus carboxypeptidase N, polypeptide 1 (Cpn1), mRNA.

Price & Availability↑

CloneID	OMu08390
Clone ID Related Accession (Same CDS sequence)	NM_030703.2
Accession Version	NM_030703.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	carboxypeptidase N catalytic chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF326477.1. On Oct 23, 2009 this sequence version replaced NM_030703.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF326477.1, AB021969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGCCAGACC TGCCCTCAGC CTTCCTGCCC CTCCTCCTCC TCTCCAAGTT TGTTACCCCG 
GTGACCTTTC GTCACCACCG CTACGACGAC CTCGTGCGGA CGCTGTACAA GGTGCACAAC 
CAATGCCCGG ACATCACGCG GCTCTACAAC ATCGGGCGCA GTGTGAAGGG GAGGTACCTC 
TACGTGCTGG AGTTTAGCGA CTACCCTGGG ATCCATGAAC CCTTGGAACC GGAAGTCAAG 
TATGTGGGAA ACATGCACGG GAACGAAGTG CTGGGCCGCG AGCTGCTTCT GCAGCTGTCG 
GAATTCCTCT GCGAGGAGTT CCGGAACCGA AACCAGCGTA TCCTGCGCCT CATTCAGGAC 
ACGCGCATTC ACATCCTGCC TTCCATGAAC CCTGACGGCT ACGAGGTGGC TGCTGCCCAG 
GGCCCAAACA TGTCTGGGTA TCTGGTTGGT AGGAACAATG CAAATGGGGT GGACCTGAAC 
CGCAACTTCC CGGATCTCAA CACCTACTTC TACTACAACT CCAAGAACGG TGGCCCTAAC 
CACCACCTGC CCCTCCCCGA CAACTGGAAA AGTCAGGTGG AACCAGAGAC CCGGGCGGTG 
ATCCAGTGGA TTCGCTCCTT GAACTTCGTT CTCTCAGCCA ATATGCACGG CGGGGCTGTG 
GTGGCCAATT ACCCATATGA CAAGTCACTT GAGCATAGGT TTCGAGGTCC CCATCGCACC 
TCCAATTCCC CGACACCTGA TGACGAGCTC TTCCAGACGC TGGCCAAGGT CTACTCCTAC 
GCCCATGGAT GGATGCACCA AGGTTGGAAC TGTGGGGACT ACTTTCCAGA CGGCATCACC 
AACGGGGCGT CCTGGTATTC TCTCAGCAAG GGAATGCAAG ACTTCAATTA TCTCCACACT 
AACTGCTTTG AGATCACGCT CGAGCTGAGT TGCGACAAGT TTCCCCGGCA AGAGGAGCTG 
CAGAGGGAGT GGCTGGGTAA CCGGGAAGCC TTAATCCAGT TCTTGGAACA GGTCCACCAG 
GGCATCAAGG GAATGGTGTT GGATGAGAAC TCTAACAACC TCACGGGGGC TGTCATTTCC 
GTCACCGGCA TCAACCACGA CGTCACTTCA GGTGAACATG GGGATTATTT CCGGCTGCTG 
CTCCCGGGTA CTTACTCTGT CACTGCCAAG GCACCAGGGT ACGACCCCAA AACAGTGACT 
GTGACGGTGG GACCTGCGGG ACCAACAGTG GTTGACTTCC AACTCAAGCG AAGCTCATCT 
CAGGTGTACC CTGTGCAGAG AGCTCCCGGC CGAGGGCAGG GAGGCAGGGC AAAGCAGCCC 
CGGACATCCA GAAAGAAAGA CCCAGCAACG AAACGGCATA GGGGTCCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109628.1
CDS	233..1606
Translation	MPDLPSAFLPLLLLSKFVTPVTFRHHRYDDLVRTLYKVHNQCPDITRLYNIGRSVKGRYLYVLEFSDYPGIHEPLEPEVKYVGNMHGNEVLGRELLLQLSEFLCEEFRNRNQRILRLIQDTRIHILPSMNPDGYEVAAAQGPNMSGYLVGRNNANGVDLNRNFPDLNTYFYYNSKNGGPNHHLPLPDNWKSQVEPETRAVIQWIRSLNFVLSANMHGGAVVANYPYDKSLEHRFRGPHRTSNSPTPDDELFQTLAKVYSYAHGWMHQGWNCGDYFPDGITNGASWYSLSKGMQDFNYLHTNCFEITLELSCDKFPRQEELQREWLGNREALIQFLEQVHQGIKGMVLDENSNNLTGAVISVTGINHDVTSGEHGDYFRLLLPGTYSVTAKAPGYDPKTVTVTVGPAGPTVVDFQLKRSSSQVYPVQRAPGRGQGGRAKQPRTSRKKDPATKRHRGPA

Target ORF information:

RefSeq Version	NM_030703.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus carboxypeptidase N, polypeptide 1 (Cpn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030703.2

ORF Insert Sequence:

ATGCCAGACC TGCCCTCAGC CTTCCTGCCC CTCCTCCTCC TCTCCAAGTT TGTTACCCCG 
GTGACCTTTC GTCACCACCG CTACGACGAC CTCGTGCGGA CGCTGTACAA GGTGCACAAC 
CAATGCCCGG ACATCACGCG GCTCTACAAC ATCGGGCGCA GTGTGAAGGG GAGGTACCTC 
TACGTGCTGG AGTTTAGCGA CTACCCTGGG ATCCATGAAC CCTTGGAACC GGAAGTCAAG 
TATGTGGGAA ACATGCACGG GAACGAAGTG CTGGGCCGCG AGCTGCTTCT GCAGCTGTCG 
GAATTCCTCT GCGAGGAGTT CCGGAACCGA AACCAGCGTA TCCTGCGCCT CATTCAGGAC 
ACGCGCATTC ACATCCTGCC TTCCATGAAC CCTGACGGCT ACGAGGTGGC TGCTGCCCAG 
GGCCCAAACA TGTCTGGGTA TCTGGTTGGT AGGAACAATG CAAATGGGGT GGACCTGAAC 
CGCAACTTCC CGGATCTCAA CACCTACTTC TACTACAACT CCAAGAACGG TGGCCCTAAC 
CACCACCTGC CCCTCCCCGA CAACTGGAAA AGTCAGGTGG AACCAGAGAC CCGGGCGGTG 
ATCCAGTGGA TTCGCTCCTT GAACTTCGTT CTCTCAGCCA ATATGCACGG CGGGGCTGTG 
GTGGCCAATT ACCCATATGA CAAGTCACTT GAGCATAGGT TTCGAGGTCC CCATCGCACC 
TCCAATTCCC CGACACCTGA TGACGAGCTC TTCCAGACGC TGGCCAAGGT CTACTCCTAC 
GCCCATGGAT GGATGCACCA AGGTTGGAAC TGTGGGGACT ACTTTCCAGA CGGCATCACC 
AACGGGGCGT CCTGGTATTC TCTCAGCAAG GGAATGCAAG ACTTCAATTA TCTCCACACT 
AACTGCTTTG AGATCACGCT CGAGCTGAGT TGCGACAAGT TTCCCCGGCA AGAGGAGCTG 
CAGAGGGAGT GGCTGGGTAA CCGGGAAGCC TTAATCCAGT TCTTGGAACA GGTCCACCAG 
GGCATCAAGG GAATGGTGTT GGATGAGAAC TCTAACAACC TCACGGGGGC TGTCATTTCC 
GTCACCGGCA TCAACCACGA CGTCACTTCA GGTGAACATG GGGATTATTT CCGGCTGCTG 
CTCCCGGGTA CTTACTCTGT CACTGCCAAG GCACCAGGGT ACGACCCCAA AACAGTGACT 
GTGACGGTGG GACCTGCGGG ACCAACAGTG GTTGACTTCC AACTCAAGCG AAGCTCATCT 
CAGGTGTACC CTGTGCAGAG AGCTCCCGGC CGAGGGCAGG GAGGCAGGGC AAAGCAGCCC 
CGGACATCCA GAAAGAAAGA CCCAGCAACG AAACGGCATA GGGGTCCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpn1 ( XM_011247407.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245709.1 Mus musculus carboxypeptidase N, polypeptide 1 (Cpn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71891
Clone ID Related Accession (Same CDS sequence)	XM_011247407.1
Accession Version	XM_011247407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	carboxypeptidase N catalytic chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGACC TGCCCTCAGC CTTCCTGCCC CTCCTCCTCC TCTCCAAGTT TGTTACCCCG 
GTGACCTTTC GTCACCACCG CTACGACGAC CTCGTGCGGA CGCTGTACAA GGTGCACAAC 
CAATGCCCGG ACATCACGCG GCTCTACAAC ATCGGGCGCA GTGTGAAGGG GAGGTACCTC 
TACGTGCTGG AGTTTAGCGA CTACCCTGGG ATCCATGAAC CCTTGGAACC GGAAGTCAAG 
TATGTGGGAA ACATGCACGG GAACGAAGTG CTGGGCCGCG AGCTGCTTCT GCAGCTGTCG 
GAATTCCTCT GCGAGGAGTT CCGGAACCGA AACCAGCGTA TCCTGCGCCT CATTCAGGAC 
ACGCGCATTC ACATCCTGCC TTCCATGAAC CCTGACGGCT ACGAGGTGGC TGCTGCCCAG 
GTACACAGTC CGAGGGAGGG CCCAAACATG TCTGGGTATC TGGTTGGTAG GAACAATGCA 
AATGGGGTGG ACCTGAACCG CAACTTCCCG GATCTCAACA CCTACTTCTA CTACAACTCC 
AAGAACGGTG GCCCTAACCA CCACCTGCCC CTCCCCGACA ACTGGAAAAG TCAGGTGGAA 
CCAGAGACCC GGGCGGTGAT CCAGTGGATT CGCTCCTTGA ACTTCGTTCT CTCAGCCAAT 
ATGCACGGCG GGGCTGTGGT GGCCAATTAC CCATATGACA AGTCACTTGA GCATAGGTTT 
CGAGGTCCCC ATCGCACCTC CAATTCCCCG ACACCTGATG ACGAGCTCTT CCAGACGCTG 
GCCAAGGTCT ACTCCTACGC CCATGGATGG ATGCACCAAG GTTGGAACTG TGGGGACTAC 
TTTCCAGACG GCATCACCAA CGGGGCGTCC TGGTATTCTC TCAGCAAGGG AATGCAAGAC 
TTCAATTATC TCCACACTAA CTGCTTTGAG ATCACGCTCG AGCTGAGTTG CGACAAGTTT 
CCCCGGCAAG AGGAGCTGCA GAGGGAGTGG CTGGGTAACC GGGAAGCCTT AATCCAGTTC 
TTGGAACAGG TCCACCAGGG CATCAAGGGA ATGGTGTTGG ATGAGAACTC TAACAACCTC 
ACGGGGGCTG TCATTTCCGT CACCGGCATC AACCACGACG TCACTTCAGG TGAACATGGG 
GATTATTTCC GGCTGCTGCT CCCGGGTACT TACTCTGTCA CTGCCAAGGC ACCAGGGTAC 
GACCCCAAAA CAGTGACTGT GACGGTGGGA CCTGCGGGAC CAACAGTGGT TGACTTCCAA 
CTCAAGCGAA GCTCATCTCA GGTGTACCCT GTGCAGAGAG CTCCCGGCCG AGGGCAGGGA 
GGCAGGGCAA AGCAGCCCCG GACATCCAGA AAGAAAGACC CAGCAACGAA ACGGCATAGG 
GGTCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245709.1
CDS	264..1655
Translation	MPDLPSAFLPLLLLSKFVTPVTFRHHRYDDLVRTLYKVHNQCPDITRLYNIGRSVKGRYLYVLEFSDYPGIHEPLEPEVKYVGNMHGNEVLGRELLLQLSEFLCEEFRNRNQRILRLIQDTRIHILPSMNPDGYEVAAAQVHSPREGPNMSGYLVGRNNANGVDLNRNFPDLNTYFYYNSKNGGPNHHLPLPDNWKSQVEPETRAVIQWIRSLNFVLSANMHGGAVVANYPYDKSLEHRFRGPHRTSNSPTPDDELFQTLAKVYSYAHGWMHQGWNCGDYFPDGITNGASWYSLSKGMQDFNYLHTNCFEITLELSCDKFPRQEELQREWLGNREALIQFLEQVHQGIKGMVLDENSNNLTGAVISVTGINHDVTSGEHGDYFRLLLPGTYSVTAKAPGYDPKTVTVTVGPAGPTVVDFQLKRSSSQVYPVQRAPGRGQGGRAKQPRTSRKKDPATKRHRGPA

Target ORF information:

RefSeq Version	XM_011247407.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus carboxypeptidase N, polypeptide 1 (Cpn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247407.1

ORF Insert Sequence:

ATGCCAGACC TGCCCTCAGC CTTCCTGCCC CTCCTCCTCC TCTCCAAGTT TGTTACCCCG 
GTGACCTTTC GTCACCACCG CTACGACGAC CTCGTGCGGA CGCTGTACAA GGTGCACAAC 
CAATGCCCGG ACATCACGCG GCTCTACAAC ATCGGGCGCA GTGTGAAGGG GAGGTACCTC 
TACGTGCTGG AGTTTAGCGA CTACCCTGGG ATCCATGAAC CCTTGGAACC GGAAGTCAAG 
TATGTGGGAA ACATGCACGG GAACGAAGTG CTGGGCCGCG AGCTGCTTCT GCAGCTGTCG 
GAATTCCTCT GCGAGGAGTT CCGGAACCGA AACCAGCGTA TCCTGCGCCT CATTCAGGAC 
ACGCGCATTC ACATCCTGCC TTCCATGAAC CCTGACGGCT ACGAGGTGGC TGCTGCCCAG 
GTACACAGTC CGAGGGAGGG CCCAAACATG TCTGGGTATC TGGTTGGTAG GAACAATGCA 
AATGGGGTGG ACCTGAACCG CAACTTCCCG GATCTCAACA CCTACTTCTA CTACAACTCC 
AAGAACGGTG GCCCTAACCA CCACCTGCCC CTCCCCGACA ACTGGAAAAG TCAGGTGGAA 
CCAGAGACCC GGGCGGTGAT CCAGTGGATT CGCTCCTTGA ACTTCGTTCT CTCAGCCAAT 
ATGCACGGCG GGGCTGTGGT GGCCAATTAC CCATATGACA AGTCACTTGA GCATAGGTTT 
CGAGGTCCCC ATCGCACCTC CAATTCCCCG ACACCTGATG ACGAGCTCTT CCAGACGCTG 
GCCAAGGTCT ACTCCTACGC CCATGGATGG ATGCACCAAG GTTGGAACTG TGGGGACTAC 
TTTCCAGACG GCATCACCAA CGGGGCGTCC TGGTATTCTC TCAGCAAGGG AATGCAAGAC 
TTCAATTATC TCCACACTAA CTGCTTTGAG ATCACGCTCG AGCTGAGTTG CGACAAGTTT 
CCCCGGCAAG AGGAGCTGCA GAGGGAGTGG CTGGGTAACC GGGAAGCCTT AATCCAGTTC 
TTGGAACAGG TCCACCAGGG CATCAAGGGA ATGGTGTTGG ATGAGAACTC TAACAACCTC 
ACGGGGGCTG TCATTTCCGT CACCGGCATC AACCACGACG TCACTTCAGG TGAACATGGG 
GATTATTTCC GGCTGCTGCT CCCGGGTACT TACTCTGTCA CTGCCAAGGC ACCAGGGTAC 
GACCCCAAAA CAGTGACTGT GACGGTGGGA CCTGCGGGAC CAACAGTGGT TGACTTCCAA 
CTCAAGCGAA GCTCATCTCA GGTGTACCCT GTGCAGAGAG CTCCCGGCCG AGGGCAGGGA 
GGCAGGGCAA AGCAGCCCCG GACATCCAGA AAGAAAGACC CAGCAACGAA ACGGCATAGG 
GGTCCTGCAT GA