Bbs7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bbs7
Entrez Gene ID	71492
Full Name	Bardet-Biedl syndrome 7 (human)
Synonyms	8430406N16Rik
General protein information	Preferred Names Bardet-Biedl syndrome 7 (human) Names Bardet-Biedl syndrome 7 protein homolog BBS2-like protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_006535535.3	XP_006535598.1	Bardet-Biedl syndrome 7 protein homolog isoform X1
XM_006535536.4	XP_006535599.1	Bardet-Biedl syndrome 7 protein homolog isoform X2
XM_006535536.3	XP_006535599.1	Bardet-Biedl syndrome 7 protein homolog isoform X2
NM_027810.3	NP_082086.2	Bardet-Biedl syndrome 7 protein homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	BBS7	NP_789794.1
	BBS7	XP_001101234.1
Caenorhabditis elegans (roundworm)	osm-12	NP_499585.1
Mus musculus (house mouse)	Bbs7	NP_082086.2
Canis lupus familiaris (dog)	BBS7	XP_533301.2
Bos taurus (cattle)	BBS7	NP_001178275.2
Gallus gallus (chicken)	BBS7	XP_003641242.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011899	XP_320627.3
Xenopus tropicalis (tropical clawed frog)	bbs7	XP_002941937.1
Pan troglodytes (chimpanzee)	BBS7	XP_003310512.1
Rattus norvegicus (Norway rat)	Bbs7	NP_001012180.1
Danio rerio (zebrafish)	bbs7	NP_001070613.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

Fine mapping a major obesity locus (jObes1) using a Berlin Fat Mouse ? B6N advanced intercross population.
Arends D, Heise S, K?rst S, Trost J, Brockmann GA
International journal of obesity (2005)40(11)1784-1788(2016 Nov)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The centriolar satellite protein AZI1 interacts with BBS4 and regulates ciliary trafficking of the BBSome.
Chamling X, Seo S, Searby CC, Kim G, Slusarski DC, Sheffield VC
PLoS genetics10(2)e1004083(2014 Feb)

BBS mutations modify phenotypic expression of CEP290-related ciliopathies.
Zhang Y, Seo S, Bhattarai S, Bugge K, Searby CC, Zhang Q, Drack AV, Stone EM, Sheffield VC
Human molecular genetics23(1)40-51(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BBS7 is not required for the localization of ciliary membrane proteins polycystin-1, polycystin-2, or bitter taste receptors, but absence of BBS7 leads to abnormal accumulation of the dopamine D1 receptor to the ciliary membrane
Title: BBS7 is required for BBSome formation and its absence in mice results in Bardet-Biedl syndrome phenotypes and selective abnormalities in membrane protein trafficking.

Knockout of Bbs7 combined with a hypomorphic Ift88 allele (orpk as a model for Shh dysfuction) results in embryonic lethality with e12.5 embryos having exencephaly, pericardial edema, cleft palate and abnormal limb development.
Title: BBS proteins interact genetically with the IFT pathway to influence SHH-related phenotypes.

The following Bbs7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bbs7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21714	NM_027810.3 Latest version!	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
OMu31422	XM_006535535.3 Latest version!	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu31423	XM_006535536.4 Latest version!	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu31423	XM_006535536.3	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bbs7 ( XM_006535536.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535599.1 Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31423
Clone ID Related Accession (Same CDS sequence)	XM_006535536.4 , XM_006535536.3
Accession Version	XM_006535536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 7 protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAA TTGAGGTTCC TGGTCCACCC ACCGTCTTAG CACTGCATAA TGGAGATGGA 
GGTGACTCTG GAGAAGGACT TCTGTTCGGC ACATCAGATG GAAGACTTGG GCTTATTCAG 
ATCACAACAT CCAAACCAAT ACACAAATGG GAAATTCGAA ATGACAAAAA GCGGGGAGGT 
ATCTTGTGTG TTGACAGCTT TGATATCATG GGTGATGGGG TTAAAGATTT ACTTGTTGGG 
AGAGATGATG GAATGGTGGA AGTATATAGT TTTGAAAATG CAAATGAACC TGTGCTACGA 
TTTGATCAGA TGCTGTCTGA AAGCGTCACC TCCATCCAGG GTGGCTGTGT GGGAAAAGAT 
GGCTATGATG AAATTGTGCT GGCCACATAT TCAGGCTGGG TGACAGGGCT GACTACAGAG 
CCAACTCATA AAGAAAGTGG ACCAGGAGAA GAACTAAAAC TTAATCAGGA AATGCAGAAT 
AAGATTTCTT CTTTAAGGTC TGAAATAGAA CACCTGCAGT TTAAGGTGCT TCAGGAGAGA 
GAAAATTACC AACAGTCTTC ACAGTCAAGC CAAGCAAAGT CAACAGTCCC TTCATTCAGT 
ATAAATGACA AGTTCACATT GAATAAAGAA GATGCTAGCT ACAGCCTCGT CTTAGAGGTG 
CGGACTGCAA TAGACAATGT CCTAATACAG AGTGATGTTC CAATAGATCT ACTTGATGTG 
GACAAGAATT CTGCTGTTGT GAGCTTCAGC AGCTGTGACA CAGAGTCAAA CGACAACTTT 
CTTCTTGCTA CTTATCGGTG CCAGGCAAAT ACCACCAGGC TGGAGCTCAA GATTCGTTCA 
ATTGAAGGCC AGTATGGCAC ACTCCAGGCA TATGTGACTC CAAGAATCCA ACCAAAAACC 
TGTCAGGTTC GCCAATACCA CATCAAACCT CTATCACTCC ATCAAAGAAC TCACTTTATT 
GACCATGACA GGCCTATGAA CACACTGACT CTAACAGGCC AGTTCAGCTT TGCTGAAGTT 
CACTCCTGGG TGGTGTTCTG CCTGCCTGAA GTTCCTGAAA AGCCTCCAGC AGGGGAATGT 
GCAACATTTT ACTTTCAGAA TACCTTTCTG GATACACAAC TTGAGTGTGT ATACAGGAAA 
GGAGAAGGAG TTTTTAAATC TGACAACATT TCTACAATAT CCATCCTAAA AGATGTGCTT 
TCTAAAGAAG CTACAAAAAG GAAAATTAAT CTCAACATAT CATACGAGAT AAACGAGGTG 
TCTGTCAAGC ACACTTTAAA GCTAATTCAC CCGAAGCTGG AGTATCAGCT GCTTCTGGCT 
AAGAAAGTGC AGTTAATCGA TGCCCTGAAG GAATTGCAGG TTCATGAGGG AAATACAGAC 
TTTCTGACTC CAGAGTACCG ATGTATTCTA GAAGAGGCAG ATCATCTACA AGAGGAATAC 
AAAAAGCAGC CTGCACATCT TGAAAGACTC TATGGTATGA TTACTGATCT TTTTATAGAT 
AAATTTAAGT TCAAAGGCAC TAATGTAAAA ACCAAAGTAC CCATGCTCCT GGAGATTCTC 
GACAGCTACG ACCAGAACAC ACTGATTTCT TTCTTCGATG CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535599.1
CDS	163..1770
Translation	MYEIEVPGPPTVLALHNGDGGDSGEGLLFGTSDGRLGLIQITTSKPIHKWEIRNDKKRGGILCVDSFDIMGDGVKDLLVGRDDGMVEVYSFENANEPVLRFDQMLSESVTSIQGGCVGKDGYDEIVLATYSGWVTGLTTEPTHKESGPGEELKLNQEMQNKISSLRSEIEHLQFKVLQERENYQQSSQSSQAKSTVPSFSINDKFTLNKEDASYSLVLEVRTAIDNVLIQSDVPIDLLDVDKNSAVVSFSSCDTESNDNFLLATYRCQANTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKPLSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECATFYFQNTFLDTQLECVYRKGEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVKHTLKLIHPKLEYQLLLAKKVQLIDALKELQVHEGNTDFLTPEYRCILEEADHLQEEYKKQPAHLERLYGMITDLFIDKFKFKGTNVKTKVPMLLEILDSYDQNTLISFFDAA

Target ORF information:

RefSeq Version	XM_006535536.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535536.3

ORF Insert Sequence:

ATGTATGAAA TTGAGGTTCC TGGTCCACCC ACCGTCTTAG CACTGCATAA TGGAGATGGA 
GGTGACTCTG GAGAAGGACT TCTGTTCGGC ACATCAGATG GAAGACTTGG GCTTATTCAG 
ATCACAACAT CCAAACCAAT ACACAAATGG GAAATTCGAA ATGACAAAAA GCGGGGAGGT 
ATCTTGTGTG TTGACAGCTT TGATATCATG GGTGATGGGG TTAAAGATTT ACTTGTTGGG 
AGAGATGATG GAATGGTGGA AGTATATAGT TTTGAAAATG CAAATGAACC TGTGCTACGA 
TTTGATCAGA TGCTGTCTGA AAGCGTCACC TCCATCCAGG GTGGCTGTGT GGGAAAAGAT 
GGCTATGATG AAATTGTGCT GGCCACATAT TCAGGCTGGG TGACAGGGCT GACTACAGAG 
CCAACTCATA AAGAAAGTGG ACCAGGAGAA GAACTAAAAC TTAATCAGGA AATGCAGAAT 
AAGATTTCTT CTTTAAGGTC TGAAATAGAA CACCTGCAGT TTAAGGTGCT TCAGGAGAGA 
GAAAATTACC AACAGTCTTC ACAGTCAAGC CAAGCAAAGT CAACAGTCCC TTCATTCAGT 
ATAAATGACA AGTTCACATT GAATAAAGAA GATGCTAGCT ACAGCCTCGT CTTAGAGGTG 
CGGACTGCAA TAGACAATGT CCTAATACAG AGTGATGTTC CAATAGATCT ACTTGATGTG 
GACAAGAATT CTGCTGTTGT GAGCTTCAGC AGCTGTGACA CAGAGTCAAA CGACAACTTT 
CTTCTTGCTA CTTATCGGTG CCAGGCAAAT ACCACCAGGC TGGAGCTCAA GATTCGTTCA 
ATTGAAGGCC AGTATGGCAC ACTCCAGGCA TATGTGACTC CAAGAATCCA ACCAAAAACC 
TGTCAGGTTC GCCAATACCA CATCAAACCT CTATCACTCC ATCAAAGAAC TCACTTTATT 
GACCATGACA GGCCTATGAA CACACTGACT CTAACAGGCC AGTTCAGCTT TGCTGAAGTT 
CACTCCTGGG TGGTGTTCTG CCTGCCTGAA GTTCCTGAAA AGCCTCCAGC AGGGGAATGT 
GCAACATTTT ACTTTCAGAA TACCTTTCTG GATACACAAC TTGAGTGTGT ATACAGGAAA 
GGAGAAGGAG TTTTTAAATC TGACAACATT TCTACAATAT CCATCCTAAA AGATGTGCTT 
TCTAAAGAAG CTACAAAAAG GAAAATTAAT CTCAACATAT CATACGAGAT AAACGAGGTG 
TCTGTCAAGC ACACTTTAAA GCTAATTCAC CCGAAGCTGG AGTATCAGCT GCTTCTGGCT 
AAGAAAGTGC AGTTAATCGA TGCCCTGAAG GAATTGCAGG TTCATGAGGG AAATACAGAC 
TTTCTGACTC CAGAGTACCG ATGTATTCTA GAAGAGGCAG ATCATCTACA AGAGGAATAC 
AAAAAGCAGC CTGCACATCT TGAAAGACTC TATGGTATGA TTACTGATCT TTTTATAGAT 
AAATTTAAGT TCAAAGGCAC TAATGTAAAA ACCAAAGTAC CCATGCTCCT GGAGATTCTC 
GACAGCTACG ACCAGAACAC ACTGATTTCT TTCTTCGATG CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bbs7 ( NM_027810.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082086.2 Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), mRNA.

Price & Availability↑

CloneID	OMu21714
Clone ID Related Accession (Same CDS sequence)	NM_027810.3
Accession Version	NM_027810.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 7 protein homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117584.11. On Mar 21, 2008 this sequence version replaced NM_027810.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031505.1, AK132661.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGATCTGA CGTTAAGCCG GGCAGACTAC CTCCAGGTCG GAGTGACCTC TCAGAAAACT 
ATGAAGTTGC TTCCTACCTC AAGACAAAGG GCTACACAAA AGGTGGTTGT TGGAGATCAA 
GATGGGGTAG TGATATGCTT CGGCGTGAAG AAAGGAGAAG CTGTGCCAGT GTTCAAGACT 
TTACCAGGGC AGAAGATCTC GAGGCTGGAA CTAGGAGGGG CCGTCAACAC GCCTCAGGAG 
AAAATCTTCA TTGCTGCAGG GTCCGAGATT AGAGGCTTTA CAAAGAGAGG GAAACAGTTT 
CTCTCTTTTG AAACTAACCT CACGGAAAGC ATTAAAGCTA TGTACATATC TGGCTCTGAC 
CTCTTCCTCA GTGCAAGCTA CATCTATAAT CACTACTGTG ATTGCAAAGA CCAAAATTAT 
TACCTTTCTG GGGACAAAAT CAATGATGTC ATCTGCCTTC CTGTGGAAAA GTTATCTCGC 
GTGACACCGG TGTTGGCCTG CCAGGACCGG GTGCTCCGAG TCTTACAGGG ATCTGATGTG 
ATGTATGAAA TTGAGGTTCC TGGTCCACCC ACCGTCTTAG CACTGCATAA TGGAGATGGA 
GGTGACTCTG GAGAAGGACT TCTGTTCGGC ACATCAGATG GAAGACTTGG GCTTATTCAG 
ATCACAACAT CCAAACCAAT ACACAAATGG GAAATTCGAA ATGACAAAAA GCGGGGAGGT 
ATCTTGTGTG TTGACAGCTT TGATATCATG GGTGATGGGG TTAAAGATTT ACTTGTTGGG 
AGAGATGATG GAATGGTGGA AGTATATAGT TTTGAAAATG CAAATGAACC TGTGCTACGA 
TTTGATCAGA TGCTGTCTGA AAGCGTCACC TCCATCCAGG GTGGCTGTGT GGGAAAAGAT 
GGCTATGATG AAATTGTGCT GGCCACATAT TCAGGCTGGG TGACAGGGCT GACTACAGAG 
CCAACTCATA AAGAAAGTGG ACCAGGAGAA GAACTAAAAC TTAATCAGGA AATGCAGAAT 
AAGATTTCTT CTTTAAGGTC TGAAATAGAA CACCTGCAGT TTAAGGTGCT TCAGGAGAGA 
GAAAATTACC AACAGTCTTC ACAGTCAAGC CAAGCAAAGT CAACAGTCCC TTCATTCAGT 
ATAAATGACA AGTTCACATT GAATAAAGAA GATGCTAGCT ACAGCCTCGT CTTAGAGGTG 
CGGACTGCAA TAGACAATGT CCTAATACAG AGTGATGTTC CAATAGATCT ACTTGATGTG 
GACAAGAATT CTGCTGTTGT GAGCTTCAGC AGCTGTGACA CAGAGTCAAA CGACAACTTT 
CTTCTTGCTA CTTATCGGTG CCAGGCAAAT ACCACCAGGC TGGAGCTCAA GATTCGTTCA 
ATTGAAGGCC AGTATGGCAC ACTCCAGGCA TATGTGACTC CAAGAATCCA ACCAAAAACC 
TGTCAGGTTC GCCAATACCA CATCAAACCT CTATCACTCC ATCAAAGAAC TCACTTTATT 
GACCATGACA GGCCTATGAA CACACTGACT CTAACAGGCC AGTTCAGCTT TGCTGAAGTT 
CACTCCTGGG TGGTGTTCTG CCTGCCTGAA GTTCCTGAAA AGCCTCCAGC AGGGGAATGT 
GCAACATTTT ACTTTCAGAA TACCTTTCTG GATACACAAC TTGAGTGTGT ATACAGGAAA 
GGAGAAGGAG TTTTTAAATC TGACAACATT TCTACAATAT CCATCCTAAA AGATGTGCTT 
TCTAAAGAAG CTACAAAAAG GAAAATTAAT CTCAACATAT CATACGAGAT AAACGAGGTG 
TCTGTCAAGC ACACTTTAAA GCTAATTCAC CCGAAGCTGG AGTATCAGCT GCTTCTGGCT 
AAGAAAGTGC AGTTAATCGA TGCCCTGAAG GAATTGCAGG TTCATGAGGG AAATACAGAC 
TTTCTGACTC CAGAGTACCG ATGTATTCTA GAAGAGGCAG ATCATCTACA AGAGGAATAC 
AAAAAGCAGC CTGCACATCT TGAAAGACTC TATGGTATGA TTACTGATCT TTTTATAGAT 
AAATTTAAGT TCAAAGGCAC TAATGTAAAA ACCAAAGTAC CCATGCTCCT GGAGATTCTC 
GACAGCTACG ACCAGAACAC ACTGATTTCT TTCTTCGATG CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082086.2
CDS	105..2252
Translation	MDLTLSRADYLQVGVTSQKTMKLLPTSRQRATQKVVVGDQDGVVICFGVKKGEAVPVFKTLPGQKISRLELGGAVNTPQEKIFIAAGSEIRGFTKRGKQFLSFETNLTESIKAMYISGSDLFLSASYIYNHYCDCKDQNYYLSGDKINDVICLPVEKLSRVTPVLACQDRVLRVLQGSDVMYEIEVPGPPTVLALHNGDGGDSGEGLLFGTSDGRLGLIQITTSKPIHKWEIRNDKKRGGILCVDSFDIMGDGVKDLLVGRDDGMVEVYSFENANEPVLRFDQMLSESVTSIQGGCVGKDGYDEIVLATYSGWVTGLTTEPTHKESGPGEELKLNQEMQNKISSLRSEIEHLQFKVLQERENYQQSSQSSQAKSTVPSFSINDKFTLNKEDASYSLVLEVRTAIDNVLIQSDVPIDLLDVDKNSAVVSFSSCDTESNDNFLLATYRCQANTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKPLSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECATFYFQNTFLDTQLECVYRKGEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVKHTLKLIHPKLEYQLLLAKKVQLIDALKELQVHEGNTDFLTPEYRCILEEADHLQEEYKKQPAHLERLYGMITDLFIDKFKFKGTNVKTKVPMLLEILDSYDQNTLISFFDAA

Target ORF information:

RefSeq Version	NM_027810.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027810.3

ORF Insert Sequence:

ATGGATCTGA CGTTAAGCCG GGCAGACTAC CTCCAGGTCG GAGTGACCTC TCAGAAAACT 
ATGAAGTTGC TTCCTACCTC AAGACAAAGG GCTACACAAA AGGTGGTTGT TGGAGATCAA 
GATGGGGTAG TGATATGCTT CGGCGTGAAG AAAGGAGAAG CTGTGCCAGT GTTCAAGACT 
TTACCAGGGC AGAAGATCTC GAGGCTGGAA CTAGGAGGGG CCGTCAACAC GCCTCAGGAG 
AAAATCTTCA TTGCTGCAGG GTCCGAGATT AGAGGCTTTA CAAAGAGAGG GAAACAGTTT 
CTCTCTTTTG AAACTAACCT CACGGAAAGC ATTAAAGCTA TGTACATATC TGGCTCTGAC 
CTCTTCCTCA GTGCAAGCTA CATCTATAAT CACTACTGTG ATTGCAAAGA CCAAAATTAT 
TACCTTTCTG GGGACAAAAT CAATGATGTC ATCTGCCTTC CTGTGGAAAA GTTATCTCGC 
GTGACACCGG TGTTGGCCTG CCAGGACCGG GTGCTCCGAG TCTTACAGGG ATCTGATGTG 
ATGTATGAAA TTGAGGTTCC TGGTCCACCC ACCGTCTTAG CACTGCATAA TGGAGATGGA 
GGTGACTCTG GAGAAGGACT TCTGTTCGGC ACATCAGATG GAAGACTTGG GCTTATTCAG 
ATCACAACAT CCAAACCAAT ACACAAATGG GAAATTCGAA ATGACAAAAA GCGGGGAGGT 
ATCTTGTGTG TTGACAGCTT TGATATCATG GGTGATGGGG TTAAAGATTT ACTTGTTGGG 
AGAGATGATG GAATGGTGGA AGTATATAGT TTTGAAAATG CAAATGAACC TGTGCTACGA 
TTTGATCAGA TGCTGTCTGA AAGCGTCACC TCCATCCAGG GTGGCTGTGT GGGAAAAGAT 
GGCTATGATG AAATTGTGCT GGCCACATAT TCAGGCTGGG TGACAGGGCT GACTACAGAG 
CCAACTCATA AAGAAAGTGG ACCAGGAGAA GAACTAAAAC TTAATCAGGA AATGCAGAAT 
AAGATTTCTT CTTTAAGGTC TGAAATAGAA CACCTGCAGT TTAAGGTGCT TCAGGAGAGA 
GAAAATTACC AACAGTCTTC ACAGTCAAGC CAAGCAAAGT CAACAGTCCC TTCATTCAGT 
ATAAATGACA AGTTCACATT GAATAAAGAA GATGCTAGCT ACAGCCTCGT CTTAGAGGTG 
CGGACTGCAA TAGACAATGT CCTAATACAG AGTGATGTTC CAATAGATCT ACTTGATGTG 
GACAAGAATT CTGCTGTTGT GAGCTTCAGC AGCTGTGACA CAGAGTCAAA CGACAACTTT 
CTTCTTGCTA CTTATCGGTG CCAGGCAAAT ACCACCAGGC TGGAGCTCAA GATTCGTTCA 
ATTGAAGGCC AGTATGGCAC ACTCCAGGCA TATGTGACTC CAAGAATCCA ACCAAAAACC 
TGTCAGGTTC GCCAATACCA CATCAAACCT CTATCACTCC ATCAAAGAAC TCACTTTATT 
GACCATGACA GGCCTATGAA CACACTGACT CTAACAGGCC AGTTCAGCTT TGCTGAAGTT 
CACTCCTGGG TGGTGTTCTG CCTGCCTGAA GTTCCTGAAA AGCCTCCAGC AGGGGAATGT 
GCAACATTTT ACTTTCAGAA TACCTTTCTG GATACACAAC TTGAGTGTGT ATACAGGAAA 
GGAGAAGGAG TTTTTAAATC TGACAACATT TCTACAATAT CCATCCTAAA AGATGTGCTT 
TCTAAAGAAG CTACAAAAAG GAAAATTAAT CTCAACATAT CATACGAGAT AAACGAGGTG 
TCTGTCAAGC ACACTTTAAA GCTAATTCAC CCGAAGCTGG AGTATCAGCT GCTTCTGGCT 
AAGAAAGTGC AGTTAATCGA TGCCCTGAAG GAATTGCAGG TTCATGAGGG AAATACAGAC 
TTTCTGACTC CAGAGTACCG ATGTATTCTA GAAGAGGCAG ATCATCTACA AGAGGAATAC 
AAAAAGCAGC CTGCACATCT TGAAAGACTC TATGGTATGA TTACTGATCT TTTTATAGAT 
AAATTTAAGT TCAAAGGCAC TAATGTAAAA ACCAAAGTAC CCATGCTCCT GGAGATTCTC 
GACAGCTACG ACCAGAACAC ACTGATTTCT TTCTTCGATG CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bbs7 ( XM_006535535.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535598.1 Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31422
Clone ID Related Accession (Same CDS sequence)	XM_006535535.3
Accession Version	XM_006535535.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 7 protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTGA CGTTAAGCCG GGCAGACTAC CTCCAGGTGG TTGTTGGAGA TCAAGATGGG 
GTAGTGATAT GCTTCGGCGT GAAGAAAGGA GAAGCTGTGC CAGTGTTCAA GACTTTACCA 
GGGCAGAAGA TCTCGAGGCT GGAACTAGGA GGGGCCGTCA ACACGCCTCA GGAGAAAATC 
TTCATTGCTG CAGGGTCCGA GATTAGAGGC TTTACAAAGA GAGGGAAACA GTTTCTCTCT 
TTTGAAACTA ACCTCACGGA AAGCATTAAA GCTATGTACA TATCTGGCTC TGACCTCTTC 
CTCAGTGCAA GCTACATCTA TAATCACTAC TGTGATTGCA AAGACCAAAA TTATTACCTT 
TCTGGGGACA AAATCAATGA TGTCATCTGC CTTCCTGTGG AAAAGTTATC TCGCGTGACA 
CCGGTGTTGG CCTGCCAGGA CCGGGTGCTC CGAGTCTTAC AGGGATCTGA TGTGATGTAT 
GAAATTGAGG TTCCTGGTCC ACCCACCGTC TTAGCACTGC ATAATGGAGA TGGAGGTGAC 
TCTGGAGAAG GACTTCTGTT CGGCACATCA GATGGAAGAC TTGGGCTTAT TCAGATCACA 
ACATCCAAAC CAATACACAA ATGGGAAATT CGAAATGACA AAAAGCGGGG AGGTATCTTG 
TGTGTTGACA GCTTTGATAT CATGGGTGAT GGGGTTAAAG ATTTACTTGT TGGGAGAGAT 
GATGGAATGG TGGAAGTATA TAGTTTTGAA AATGCAAATG AACCTGTGCT ACGATTTGAT 
CAGATGCTGT CTGAAAGCGT CACCTCCATC CAGGGTGGCT GTGTGGGAAA AGATGGCTAT 
GATGAAATTG TGCTGGCCAC ATATTCAGGC TGGGTGACAG GGCTGACTAC AGAGCCAACT 
CATAAAGAAA GTGGACCAGG AGAAGAACTA AAACTTAATC AGGAAATGCA GAATAAGATT 
TCTTCTTTAA GGTCTGAAAT AGAACACCTG CAGTTTAAGG TGCTTCAGGA GAGAGAAAAT 
TACCAACAGT CTTCACAGTC AAGCCAAGCA AAGTCAACAG TCCCTTCATT CAGTATAAAT 
GACAAGTTCA CATTGAATAA AGAAGATGCT AGCTACAGCC TCGTCTTAGA GGTGCGGACT 
GCAATAGACA ATGTCCTAAT ACAGAGTGAT GTTCCAATAG ATCTACTTGA TGTGGACAAG 
AATTCTGCTG TTGTGAGCTT CAGCAGCTGT GACACAGAGT CAAACGACAA CTTTCTTCTT 
GCTACTTATC GGTGCCAGGC AAATACCACC AGGCTGGAGC TCAAGATTCG TTCAATTGAA 
GGCCAGTATG GCACACTCCA GGCATATGTG ACTCCAAGAA TCCAACCAAA AACCTGTCAG 
GTTCGCCAAT ACCACATCAA ACCTCTATCA CTCCATCAAA GAACTCACTT TATTGACCAT 
GACAGGCCTA TGAACACACT GACTCTAACA GGCCAGTTCA GCTTTGCTGA AGTTCACTCC 
TGGGTGGTGT TCTGCCTGCC TGAAGTTCCT GAAAAGCCTC CAGCAGGGGA ATGTGCAACA 
TTTTACTTTC AGAATACCTT TCTGGATACA CAACTTGAGT GTGTATACAG GAAAGGAGAA 
GGAGTTTTTA AATCTGACAA CATTTCTACA ATATCCATCC TAAAAGATGT GCTTTCTAAA 
GAAGCTACAA AAAGGAAAAT TAATCTCAAC ATATCATACG AGATAAACGA GGTGTCTGTC 
AAGCACACTT TAAAGCTAAT TCACCCGAAG CTGGAGTATC AGCTGCTTCT GGCTAAGAAA 
GTGCAGTTAA TCGATGCCCT GAAGGAATTG CAGGTTCATG AGGGAAATAC AGACTTTCTG 
ACTCCAGAGT ACCGATGTAT TCTAGAAGAG GCAGATCATC TACAAGAGGA ATACAAAAAG 
CAGCCTGCAC ATCTTGAAAG ACTCTATGGT ATGATTACTG ATCTTTTTAT AGATAAATTT 
AAGTTCAAAG GCACTAATGT AAAAACCAAA GTACCCATGC TCCTGGAGAT TCTCGACAGC 
TACGACCAGA ACACACTGAT TTCTTTCTTC GATGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535598.1
CDS	208..2289
Translation	MDLTLSRADYLQVVVGDQDGVVICFGVKKGEAVPVFKTLPGQKISRLELGGAVNTPQEKIFIAAGSEIRGFTKRGKQFLSFETNLTESIKAMYISGSDLFLSASYIYNHYCDCKDQNYYLSGDKINDVICLPVEKLSRVTPVLACQDRVLRVLQGSDVMYEIEVPGPPTVLALHNGDGGDSGEGLLFGTSDGRLGLIQITTSKPIHKWEIRNDKKRGGILCVDSFDIMGDGVKDLLVGRDDGMVEVYSFENANEPVLRFDQMLSESVTSIQGGCVGKDGYDEIVLATYSGWVTGLTTEPTHKESGPGEELKLNQEMQNKISSLRSEIEHLQFKVLQERENYQQSSQSSQAKSTVPSFSINDKFTLNKEDASYSLVLEVRTAIDNVLIQSDVPIDLLDVDKNSAVVSFSSCDTESNDNFLLATYRCQANTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKPLSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECATFYFQNTFLDTQLECVYRKGEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVKHTLKLIHPKLEYQLLLAKKVQLIDALKELQVHEGNTDFLTPEYRCILEEADHLQEEYKKQPAHLERLYGMITDLFIDKFKFKGTNVKTKVPMLLEILDSYDQNTLISFFDAA

Target ORF information:

RefSeq Version	XM_006535535.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535535.3

ORF Insert Sequence:

ATGGATCTGA CGTTAAGCCG GGCAGACTAC CTCCAGGTGG TTGTTGGAGA TCAAGATGGG 
GTAGTGATAT GCTTCGGCGT GAAGAAAGGA GAAGCTGTGC CAGTGTTCAA GACTTTACCA 
GGGCAGAAGA TCTCGAGGCT GGAACTAGGA GGGGCCGTCA ACACGCCTCA GGAGAAAATC 
TTCATTGCTG CAGGGTCCGA GATTAGAGGC TTTACAAAGA GAGGGAAACA GTTTCTCTCT 
TTTGAAACTA ACCTCACGGA AAGCATTAAA GCTATGTACA TATCTGGCTC TGACCTCTTC 
CTCAGTGCAA GCTACATCTA TAATCACTAC TGTGATTGCA AAGACCAAAA TTATTACCTT 
TCTGGGGACA AAATCAATGA TGTCATCTGC CTTCCTGTGG AAAAGTTATC TCGCGTGACA 
CCGGTGTTGG CCTGCCAGGA CCGGGTGCTC CGAGTCTTAC AGGGATCTGA TGTGATGTAT 
GAAATTGAGG TTCCTGGTCC ACCCACCGTC TTAGCACTGC ATAATGGAGA TGGAGGTGAC 
TCTGGAGAAG GACTTCTGTT CGGCACATCA GATGGAAGAC TTGGGCTTAT TCAGATCACA 
ACATCCAAAC CAATACACAA ATGGGAAATT CGAAATGACA AAAAGCGGGG AGGTATCTTG 
TGTGTTGACA GCTTTGATAT CATGGGTGAT GGGGTTAAAG ATTTACTTGT TGGGAGAGAT 
GATGGAATGG TGGAAGTATA TAGTTTTGAA AATGCAAATG AACCTGTGCT ACGATTTGAT 
CAGATGCTGT CTGAAAGCGT CACCTCCATC CAGGGTGGCT GTGTGGGAAA AGATGGCTAT 
GATGAAATTG TGCTGGCCAC ATATTCAGGC TGGGTGACAG GGCTGACTAC AGAGCCAACT 
CATAAAGAAA GTGGACCAGG AGAAGAACTA AAACTTAATC AGGAAATGCA GAATAAGATT 
TCTTCTTTAA GGTCTGAAAT AGAACACCTG CAGTTTAAGG TGCTTCAGGA GAGAGAAAAT 
TACCAACAGT CTTCACAGTC AAGCCAAGCA AAGTCAACAG TCCCTTCATT CAGTATAAAT 
GACAAGTTCA CATTGAATAA AGAAGATGCT AGCTACAGCC TCGTCTTAGA GGTGCGGACT 
GCAATAGACA ATGTCCTAAT ACAGAGTGAT GTTCCAATAG ATCTACTTGA TGTGGACAAG 
AATTCTGCTG TTGTGAGCTT CAGCAGCTGT GACACAGAGT CAAACGACAA CTTTCTTCTT 
GCTACTTATC GGTGCCAGGC AAATACCACC AGGCTGGAGC TCAAGATTCG TTCAATTGAA 
GGCCAGTATG GCACACTCCA GGCATATGTG ACTCCAAGAA TCCAACCAAA AACCTGTCAG 
GTTCGCCAAT ACCACATCAA ACCTCTATCA CTCCATCAAA GAACTCACTT TATTGACCAT 
GACAGGCCTA TGAACACACT GACTCTAACA GGCCAGTTCA GCTTTGCTGA AGTTCACTCC 
TGGGTGGTGT TCTGCCTGCC TGAAGTTCCT GAAAAGCCTC CAGCAGGGGA ATGTGCAACA 
TTTTACTTTC AGAATACCTT TCTGGATACA CAACTTGAGT GTGTATACAG GAAAGGAGAA 
GGAGTTTTTA AATCTGACAA CATTTCTACA ATATCCATCC TAAAAGATGT GCTTTCTAAA 
GAAGCTACAA AAAGGAAAAT TAATCTCAAC ATATCATACG AGATAAACGA GGTGTCTGTC 
AAGCACACTT TAAAGCTAAT TCACCCGAAG CTGGAGTATC AGCTGCTTCT GGCTAAGAAA 
GTGCAGTTAA TCGATGCCCT GAAGGAATTG CAGGTTCATG AGGGAAATAC AGACTTTCTG 
ACTCCAGAGT ACCGATGTAT TCTAGAAGAG GCAGATCATC TACAAGAGGA ATACAAAAAG 
CAGCCTGCAC ATCTTGAAAG ACTCTATGGT ATGATTACTG ATCTTTTTAT AGATAAATTT 
AAGTTCAAAG GCACTAATGT AAAAACCAAA GTACCCATGC TCCTGGAGAT TCTCGACAGC 
TACGACCAGA ACACACTGAT TTCTTTCTTC GATGCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bbs7 ( XM_006535536.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535599.1 Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X2, mRNA.

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CloneID	OMu31423
Clone ID Related Accession (Same CDS sequence)	XM_006535536.4 , XM_006535536.3
Accession Version	XM_006535536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 7 protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535536.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAA TTGAGGTTCC TGGTCCACCC ACCGTCTTAG CACTGCATAA TGGAGATGGA 
GGTGACTCTG GAGAAGGACT TCTGTTCGGC ACATCAGATG GAAGACTTGG GCTTATTCAG 
ATCACAACAT CCAAACCAAT ACACAAATGG GAAATTCGAA ATGACAAAAA GCGGGGAGGT 
ATCTTGTGTG TTGACAGCTT TGATATCATG GGTGATGGGG TTAAAGATTT ACTTGTTGGG 
AGAGATGATG GAATGGTGGA AGTATATAGT TTTGAAAATG CAAATGAACC TGTGCTACGA 
TTTGATCAGA TGCTGTCTGA AAGCGTCACC TCCATCCAGG GTGGCTGTGT GGGAAAAGAT 
GGCTATGATG AAATTGTGCT GGCCACATAT TCAGGCTGGG TGACAGGGCT GACTACAGAG 
CCAACTCATA AAGAAAGTGG ACCAGGAGAA GAACTAAAAC TTAATCAGGA AATGCAGAAT 
AAGATTTCTT CTTTAAGGTC TGAAATAGAA CACCTGCAGT TTAAGGTGCT TCAGGAGAGA 
GAAAATTACC AACAGTCTTC ACAGTCAAGC CAAGCAAAGT CAACAGTCCC TTCATTCAGT 
ATAAATGACA AGTTCACATT GAATAAAGAA GATGCTAGCT ACAGCCTCGT CTTAGAGGTG 
CGGACTGCAA TAGACAATGT CCTAATACAG AGTGATGTTC CAATAGATCT ACTTGATGTG 
GACAAGAATT CTGCTGTTGT GAGCTTCAGC AGCTGTGACA CAGAGTCAAA CGACAACTTT 
CTTCTTGCTA CTTATCGGTG CCAGGCAAAT ACCACCAGGC TGGAGCTCAA GATTCGTTCA 
ATTGAAGGCC AGTATGGCAC ACTCCAGGCA TATGTGACTC CAAGAATCCA ACCAAAAACC 
TGTCAGGTTC GCCAATACCA CATCAAACCT CTATCACTCC ATCAAAGAAC TCACTTTATT 
GACCATGACA GGCCTATGAA CACACTGACT CTAACAGGCC AGTTCAGCTT TGCTGAAGTT 
CACTCCTGGG TGGTGTTCTG CCTGCCTGAA GTTCCTGAAA AGCCTCCAGC AGGGGAATGT 
GCAACATTTT ACTTTCAGAA TACCTTTCTG GATACACAAC TTGAGTGTGT ATACAGGAAA 
GGAGAAGGAG TTTTTAAATC TGACAACATT TCTACAATAT CCATCCTAAA AGATGTGCTT 
TCTAAAGAAG CTACAAAAAG GAAAATTAAT CTCAACATAT CATACGAGAT AAACGAGGTG 
TCTGTCAAGC ACACTTTAAA GCTAATTCAC CCGAAGCTGG AGTATCAGCT GCTTCTGGCT 
AAGAAAGTGC AGTTAATCGA TGCCCTGAAG GAATTGCAGG TTCATGAGGG AAATACAGAC 
TTTCTGACTC CAGAGTACCG ATGTATTCTA GAAGAGGCAG ATCATCTACA AGAGGAATAC 
AAAAAGCAGC CTGCACATCT TGAAAGACTC TATGGTATGA TTACTGATCT TTTTATAGAT 
AAATTTAAGT TCAAAGGCAC TAATGTAAAA ACCAAAGTAC CCATGCTCCT GGAGATTCTC 
GACAGCTACG ACCAGAACAC ACTGATTTCT TTCTTCGATG CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535599.1
CDS	162..1769
Translation	MYEIEVPGPPTVLALHNGDGGDSGEGLLFGTSDGRLGLIQITTSKPIHKWEIRNDKKRGGILCVDSFDIMGDGVKDLLVGRDDGMVEVYSFENANEPVLRFDQMLSESVTSIQGGCVGKDGYDEIVLATYSGWVTGLTTEPTHKESGPGEELKLNQEMQNKISSLRSEIEHLQFKVLQERENYQQSSQSSQAKSTVPSFSINDKFTLNKEDASYSLVLEVRTAIDNVLIQSDVPIDLLDVDKNSAVVSFSSCDTESNDNFLLATYRCQANTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKPLSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECATFYFQNTFLDTQLECVYRKGEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVKHTLKLIHPKLEYQLLLAKKVQLIDALKELQVHEGNTDFLTPEYRCILEEADHLQEEYKKQPAHLERLYGMITDLFIDKFKFKGTNVKTKVPMLLEILDSYDQNTLISFFDAA

Target ORF information:

RefSeq Version	XM_006535536.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 7 (human) (Bbs7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535536.4

ORF Insert Sequence:

ATGTATGAAA TTGAGGTTCC TGGTCCACCC ACCGTCTTAG CACTGCATAA TGGAGATGGA 
GGTGACTCTG GAGAAGGACT TCTGTTCGGC ACATCAGATG GAAGACTTGG GCTTATTCAG 
ATCACAACAT CCAAACCAAT ACACAAATGG GAAATTCGAA ATGACAAAAA GCGGGGAGGT 
ATCTTGTGTG TTGACAGCTT TGATATCATG GGTGATGGGG TTAAAGATTT ACTTGTTGGG 
AGAGATGATG GAATGGTGGA AGTATATAGT TTTGAAAATG CAAATGAACC TGTGCTACGA 
TTTGATCAGA TGCTGTCTGA AAGCGTCACC TCCATCCAGG GTGGCTGTGT GGGAAAAGAT 
GGCTATGATG AAATTGTGCT GGCCACATAT TCAGGCTGGG TGACAGGGCT GACTACAGAG 
CCAACTCATA AAGAAAGTGG ACCAGGAGAA GAACTAAAAC TTAATCAGGA AATGCAGAAT 
AAGATTTCTT CTTTAAGGTC TGAAATAGAA CACCTGCAGT TTAAGGTGCT TCAGGAGAGA 
GAAAATTACC AACAGTCTTC ACAGTCAAGC CAAGCAAAGT CAACAGTCCC TTCATTCAGT 
ATAAATGACA AGTTCACATT GAATAAAGAA GATGCTAGCT ACAGCCTCGT CTTAGAGGTG 
CGGACTGCAA TAGACAATGT CCTAATACAG AGTGATGTTC CAATAGATCT ACTTGATGTG 
GACAAGAATT CTGCTGTTGT GAGCTTCAGC AGCTGTGACA CAGAGTCAAA CGACAACTTT 
CTTCTTGCTA CTTATCGGTG CCAGGCAAAT ACCACCAGGC TGGAGCTCAA GATTCGTTCA 
ATTGAAGGCC AGTATGGCAC ACTCCAGGCA TATGTGACTC CAAGAATCCA ACCAAAAACC 
TGTCAGGTTC GCCAATACCA CATCAAACCT CTATCACTCC ATCAAAGAAC TCACTTTATT 
GACCATGACA GGCCTATGAA CACACTGACT CTAACAGGCC AGTTCAGCTT TGCTGAAGTT 
CACTCCTGGG TGGTGTTCTG CCTGCCTGAA GTTCCTGAAA AGCCTCCAGC AGGGGAATGT 
GCAACATTTT ACTTTCAGAA TACCTTTCTG GATACACAAC TTGAGTGTGT ATACAGGAAA 
GGAGAAGGAG TTTTTAAATC TGACAACATT TCTACAATAT CCATCCTAAA AGATGTGCTT 
TCTAAAGAAG CTACAAAAAG GAAAATTAAT CTCAACATAT CATACGAGAT AAACGAGGTG 
TCTGTCAAGC ACACTTTAAA GCTAATTCAC CCGAAGCTGG AGTATCAGCT GCTTCTGGCT 
AAGAAAGTGC AGTTAATCGA TGCCCTGAAG GAATTGCAGG TTCATGAGGG AAATACAGAC 
TTTCTGACTC CAGAGTACCG ATGTATTCTA GAAGAGGCAG ATCATCTACA AGAGGAATAC 
AAAAAGCAGC CTGCACATCT TGAAAGACTC TATGGTATGA TTACTGATCT TTTTATAGAT 
AAATTTAAGT TCAAAGGCAC TAATGTAAAA ACCAAAGTAC CCATGCTCCT GGAGATTCTC 
GACAGCTACG ACCAGAACAC ACTGATTTCT TTCTTCGATG CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

Fine mapping a major obesity locus (jObes1) using a Berlin Fat Mouse ? B6N advanced intercross population.
International journal of obesity (2005)40(11)1784-1788(2016 Nov)
Arends D,Heise S,K?rst S,Trost J,Brockmann GA

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The centriolar satellite protein AZI1 interacts with BBS4 and regulates ciliary trafficking of the BBSome.
PLoS genetics10(2)e1004083(2014 Feb)
Chamling X,Seo S,Searby CC,Kim G,Slusarski DC,Sheffield VC

BBS mutations modify phenotypic expression of CEP290-related ciliopathies.
Human molecular genetics23(1)40-51(2014 Jan)
Zhang Y,Seo S,Bhattarai S,Bugge K,Searby CC,Zhang Q,Drack AV,Stone EM,Sheffield VC

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Bbs7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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