Sept12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sept12
Entrez Gene ID	71089
Full Name	septin 12
Synonyms	1700028G04Rik,4933413B09Rik,Septin12
General protein information	Preferred Names septin 12 Names septin-12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_027669.3	NP_081945.1	septin-12 isoform 1
XM_006522580.1	XP_006522643.1	septin-12 isoform X4
NM_029374.1	NP_083650.1	septin-12 isoform 2
XM_006522578.2	XP_006522641.1	septin-12 isoform X2
XM_006522579.3	XP_006522642.1	septin-12 isoform X3
NM_001373945.1	NP_001360874.1	septin-12 isoform 3
XM_006522577.2	XP_006522640.1	septin-12 isoform X1

Pathways from BioSystems

Homologies

	LOC706521	XP_002808333.1
Homo sapiens (human)	SEPT12	NP_653206.2
Pan troglodytes (chimpanzee)	SEPT12	XP_001169473.2
Canis lupus familiaris (dog)	SEPT12	XP_003434910.1
Xenopus tropicalis (tropical clawed frog)	sept12	NP_001025542.1
Bos taurus (cattle)	SEPT12	NP_001091612.1
Mus musculus (house mouse)	Sept12	NP_081945.1
Gallus gallus (chicken)	SEPT12	XP_004945472.1
Danio rerio (zebrafish)	sept12	XP_693022.3
Rattus norvegicus (Norway rat)	Sept12	NP_001094335.1

Gene Ontology
Bibliography

Related articles in PubMed

SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.
Shen YR, Wang HY, Kuo YC, Shih SC, Hsu CH, Chen YR, Wu SR, Wang CY, Kuo PL
PLoS genetics13(3)e1006631(2017 Mar)

SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.
Lai TH, Wu YY, Wang YY, Chen MF, Wang P, Chen TM, Wu YN, Chiang HS, Kuo PL, Lin YH
International journal of molecular sciences17(11)(2016 Nov)

SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.
Kuo YC, Shen YR, Chen HI, Lin YH, Wang YY, Chen YR, Wang CY, Kuo PL
Journal of cell science128(5)923-34(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that SEPT12 phosphorylation inhibits septin ring assembly during annulus biogenesis.
Title: SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.

In mice sperm with mutated SEPT12, NDC1 dispersed around the manchette region of the sperm head and annulus, compared with concentrating at the sperm neck of wild-type sperm.
Title: SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.

results demonstrate the molecular architecture of SEPT12 filaments at the sperm annulus, their mechanical support of sperm motility, and their correlation with male infertility.
Title: SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.

Oocytes fertilized with spermatozoa obtained from Septin 12+/- chimeric mice failed to develop beyond the morula stage after IVF and intracytoplasmic sperm injection because of significant DNA defects in the spermatozoa
Title: SEPT12 deficiency causes sperm nucleus damage and developmental arrest of preimplantation embryos.

The following Sept12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sept12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04480	NM_027669.3 Latest version!	Mus musculus septin 12 (Septin12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu06897	NM_029374.1 Latest version!	Mus musculus septin 12 (Septin12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu52409	XM_006522577.2 Latest version!	Mus musculus septin 12 (Sept12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu52410	XM_006522578.2 Latest version!	Mus musculus septin 12 (Sept12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu52411	XM_006522579.3 Latest version!	Mus musculus septin 12 (Sept12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu52412	XM_006522580.1 Latest version!	Mus musculus septin 12 (Sept12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu52410	NM_001373945.1 Latest version!	Mus musculus septin 12 (Septin12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sept12 ( NM_027669.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081945.1 Mus musculus septin 12 (Septin12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04480
Clone ID Related Accession (Same CDS sequence)	NM_027669.3
Accession Version	NM_027669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	septin-12 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077091.1. On Feb 3, 2011 this sequence version replaced NM_027669.2. Transcript Variant: This variant (1) encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK077091.1, AK016798.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGGTA AGTTTGGGAA CATTGCAGGC 
ATGCAATGGG TAGGGGGTTG GAAATCAGGG CATACCCTAC ATGCATGGCA TCCTATGCAT 
GCCTCCCCTG GCGTCTGCCC AACCTGGATG GCAACCATGA CTTGCAAAAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081945.1
CDS	139..1092
Translation	MDERRSPSPCSSRPSSPRTPPNEMFGPVGIEAVLDQLRIKAMKTGFEFNIMVVGQSGLGKSTMVNTLFKSKVWQSTEPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKTHCIDVYPQKCFDEDVNDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEGKFGNIAGMQWVGGWKSGHTLHAWHPMHASPGVCPTWMATMTCKT

Target ORF information:

RefSeq Version	NM_027669.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus septin 12 (Septin12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027669.3

ORF Insert Sequence:

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGGTA AGTTTGGGAA CATTGCAGGC 
ATGCAATGGG TAGGGGGTTG GAAATCAGGG CATACCCTAC ATGCATGGCA TCCTATGCAT 
GCCTCCCCTG GCGTCTGCCC AACCTGGATG GCAACCATGA CTTGCAAAAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sept12 ( NM_029374.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083650.1 Mus musculus septin 12 (Septin12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06897
Clone ID Related Accession (Same CDS sequence)	NM_029374.1
Accession Version	NM_029374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	septin-12 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077091.1 and AK006458.1. Transcript Variant: This variant (2) uses two alternate splice sites in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK006458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGCTC CCATCTCCAG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083650.1
CDS	139..975
Translation	MDERRSPSPCSSRPSSPRTPPNEMFGPVGIEAVLDQLRIKAMKTGFEFNIMVVGQSGLGKSTMVNTLFKSKVWQSTEPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKTHCIDVYPQKCFDEDVNDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEAPISRT

Target ORF information:

RefSeq Version	NM_029374.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus septin 12 (Septin12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029374.1

ORF Insert Sequence:

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGCTC CCATCTCCAG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sept12 ( XM_006522577.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522640.1 Mus musculus septin 12 (Sept12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52409
Clone ID Related Accession (Same CDS sequence)	XM_006522577.2
Accession Version	XM_006522577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	septin-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGGCC CATGTCTTGC AGTGGAAAAC 
ATGGCGCACT GTGAGTTTCT TCTCCTGAGA GATCTGCTCA TCCGCTCCCA TCTCCAGGAC 
CTGAAGGACA TCACCCACAA TGTACACTAC GAGAACTACC GGGTACTCAG ACTCAATGAG 
AGCCACGTGC TGCCCCGGGG GCCAGGCTGG GTGAACCTGG CTCCAGTCTC CCCTGGACAG 
CTGATGGCCC CTGGACCGGG AAAGGTCCGA AAGCGAGCAA ACAAGGATAC CAAGGACGAA 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522640.1
CDS	134..1222
Translation	MDERRSPSPCSSRPSSPRTPPNEMFGPVGIEAVLDQLRIKAMKTGFEFNIMVVGQSGLGKSTMVNTLFKSKVWQSTEPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKTHCIDVYPQKCFDEDVNDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEGPCLAVENMAHCEFLLLRDLLIRSHLQDLKDITHNVHYENYRVLRLNESHVLPRGPGWVNLAPVSPGQLMAPGPGKVRKRANKDTKDEEC

Target ORF information:

RefSeq Version	XM_006522577.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus septin 12 (Sept12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522577.2

ORF Insert Sequence:

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGGCC CATGTCTTGC AGTGGAAAAC 
ATGGCGCACT GTGAGTTTCT TCTCCTGAGA GATCTGCTCA TCCGCTCCCA TCTCCAGGAC 
CTGAAGGACA TCACCCACAA TGTACACTAC GAGAACTACC GGGTACTCAG ACTCAATGAG 
AGCCACGTGC TGCCCCGGGG GCCAGGCTGG GTGAACCTGG CTCCAGTCTC CCCTGGACAG 
CTGATGGCCC CTGGACCGGG AAAGGTCCGA AAGCGAGCAA ACAAGGATAC CAAGGACGAA 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sept12 ( XM_006522578.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522641.1 Mus musculus septin 12 (Sept12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu52410
Clone ID Related Accession (Same CDS sequence)	XM_006522578.2 , NM_001373945.1
Accession Version	XM_006522578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	septin-12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGTGG AAAACATGGC GCACTGTGAG 
TTTCTTCTCC TGAGAGATCT GCTCATCCGC TCCCATCTCC AGGACCTGAA GGACATCACC 
CACAATGTAC ACTACGAGAA CTACCGGGTA CTCAGACTCA ATGAGAGCCA CGTGCTGCCC 
CGGGGGCCAG GCTGGGTGAA CCTGGCTCCA GTCTCCCCTG GACAGCTGAT GGCCCCTGGA 
CCGGGAAAGG TCCGAAAGCG AGCAAACAAG GATACCAAGG ACGAAGAGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522641.1
CDS	134..1207
Translation	MDERRSPSPCSSRPSSPRTPPNEMFGPVGIEAVLDQLRIKAMKTGFEFNIMVVGQSGLGKSTMVNTLFKSKVWQSTEPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKTHCIDVYPQKCFDEDVNDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEVENMAHCEFLLLRDLLIRSHLQDLKDITHNVHYENYRVLRLNESHVLPRGPGWVNLAPVSPGQLMAPGPGKVRKRANKDTKDEEC

Target ORF information:

RefSeq Version	XM_006522578.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus septin 12 (Sept12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522578.2

ORF Insert Sequence:

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGTGG AAAACATGGC GCACTGTGAG 
TTTCTTCTCC TGAGAGATCT GCTCATCCGC TCCCATCTCC AGGACCTGAA GGACATCACC 
CACAATGTAC ACTACGAGAA CTACCGGGTA CTCAGACTCA ATGAGAGCCA CGTGCTGCCC 
CGGGGGCCAG GCTGGGTGAA CCTGGCTCCA GTCTCCCCTG GACAGCTGAT GGCCCCTGGA 
CCGGGAAAGG TCCGAAAGCG AGCAAACAAG GATACCAAGG ACGAAGAGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sept12 ( XM_006522579.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522642.1 Mus musculus septin 12 (Sept12), transcript variant X3, mRNA.

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CloneID	OMu52411
Clone ID Related Accession (Same CDS sequence)	XM_006522579.3
Accession Version	XM_006522579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	septin-12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CACTGTTCAA GTCCAAAGTA TGGCAGTCAA CTGAACCCAA CCTGGATGTA 
CCCATGCCAC AAACATTGGA GCTGCACTCT GTGACCCATG TCATCGAGGA GAAGGGATTG 
AAGCTGAAGC TGACAGTGAC AGACACACCT GGCTTTGGAG ACCAGATCAA CAATGACAAG 
TGCTGGGACC CTATCCTGAG CTACATCAAC CAACAGTATG AGCAGTATCT ACAAGAGGAG 
CTCCTGATTA CCCGCCAGCG CCACATCCCC GACACCCGTG TGCACTGCTG TGTGTACTTT 
GTGCCACCCA CTGGCCACTG CCTACGGCCC CTAGACATTG AGTTCCTGAG GCGGCTGTGC 
CGGACTGTGA ATGTGGTGCC GGTGATCGCC CGGGCTGACA GCCTGACCAT TGAAGAACGA 
GATGCCTTCA GGAGCAGGAT CCAGCAAAAC CTGAAAACTC ACTGCATCGA TGTGTACCCA 
CAGAAGTGTT TCGACGAGGA TGTCAATGAC AGACTTCTCA ACAGTAAGAT TCGGGAGCAG 
ATCCCTTTTG CTGTGGTCGG GGCTGACCGA GAGCACATAG TGAATGGGAG ATGTGTCCTG 
GGCCGGAAGA CCAAATGGGG CATCATCGAA GGCCCATGTC TTGCAGTGGA AAACATGGCG 
CACTGTGAGT TTCTTCTCCT GAGAGATCTG CTCATCCGCT CCCATCTCCA GGACCTGAAG 
GACATCACCC ACAATGTACA CTACGAGAAC TACCGGGTAC TCAGACTCAA TGAGAGCCAC 
GTGCTGCCCC GGGGGCCAGG CTGGGTGAAC CTGGCTCCAG TCTCCCCTGG ACAGCTGATG 
GCCCCTGGAC CGGGAAAGGT CCGAAAGCGA GCAAACAAGG ATACCAAGGA CGAAGAGTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522642.1
CDS	131..1033
Translation	MVNTLFKSKVWQSTEPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKTHCIDVYPQKCFDEDVNDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEGPCLAVENMAHCEFLLLRDLLIRSHLQDLKDITHNVHYENYRVLRLNESHVLPRGPGWVNLAPVSPGQLMAPGPGKVRKRANKDTKDEEC

Target ORF information:

RefSeq Version	XM_006522579.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus septin 12 (Sept12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522579.3

ORF Insert Sequence:

ATGGTGAACA CACTGTTCAA GTCCAAAGTA TGGCAGTCAA CTGAACCCAA CCTGGATGTA 
CCCATGCCAC AAACATTGGA GCTGCACTCT GTGACCCATG TCATCGAGGA GAAGGGATTG 
AAGCTGAAGC TGACAGTGAC AGACACACCT GGCTTTGGAG ACCAGATCAA CAATGACAAG 
TGCTGGGACC CTATCCTGAG CTACATCAAC CAACAGTATG AGCAGTATCT ACAAGAGGAG 
CTCCTGATTA CCCGCCAGCG CCACATCCCC GACACCCGTG TGCACTGCTG TGTGTACTTT 
GTGCCACCCA CTGGCCACTG CCTACGGCCC CTAGACATTG AGTTCCTGAG GCGGCTGTGC 
CGGACTGTGA ATGTGGTGCC GGTGATCGCC CGGGCTGACA GCCTGACCAT TGAAGAACGA 
GATGCCTTCA GGAGCAGGAT CCAGCAAAAC CTGAAAACTC ACTGCATCGA TGTGTACCCA 
CAGAAGTGTT TCGACGAGGA TGTCAATGAC AGACTTCTCA ACAGTAAGAT TCGGGAGCAG 
ATCCCTTTTG CTGTGGTCGG GGCTGACCGA GAGCACATAG TGAATGGGAG ATGTGTCCTG 
GGCCGGAAGA CCAAATGGGG CATCATCGAA GGCCCATGTC TTGCAGTGGA AAACATGGCG 
CACTGTGAGT TTCTTCTCCT GAGAGATCTG CTCATCCGCT CCCATCTCCA GGACCTGAAG 
GACATCACCC ACAATGTACA CTACGAGAAC TACCGGGTAC TCAGACTCAA TGAGAGCCAC 
GTGCTGCCCC GGGGGCCAGG CTGGGTGAAC CTGGCTCCAG TCTCCCCTGG ACAGCTGATG 
GCCCCTGGAC CGGGAAAGGT CCGAAAGCGA GCAAACAAGG ATACCAAGGA CGAAGAGTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sept12 ( XM_006522580.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522643.1 Mus musculus septin 12 (Sept12), transcript variant X4, mRNA.

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CloneID	OMu52412
Clone ID Related Accession (Same CDS sequence)	XM_006522580.1
Accession Version	XM_006522580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	septin-12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGGAC CTGAAGGACA TCACCCACAA 
TGTACACTAC GAGAACTACC GGGTACTCAG ACTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522643.1
CDS	134..1012
Translation	MDERRSPSPCSSRPSSPRTPPNEMFGPVGIEAVLDQLRIKAMKTGFEFNIMVVGQSGLGKSTMVNTLFKSKVWQSTEPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKTHCIDVYPQKCFDEDVNDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEGPEGHHPQCTLRELPGTQTQ

Target ORF information:

RefSeq Version	XM_006522580.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus septin 12 (Sept12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522580.1

ORF Insert Sequence:

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGGAC CTGAAGGACA TCACCCACAA 
TGTACACTAC GAGAACTACC GGGTACTCAG ACTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sept12 ( NM_001373945.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001360874.1 Mus musculus septin 12 (Septin12), transcript variant 3, mRNA.

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CloneID	OMu52410
Clone ID Related Accession (Same CDS sequence)	XM_006522578.2 , NM_001373945.1
Accession Version	NM_001373945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	septin-12 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163723.3. On Sep 5, 2019 this sequence version replaced XM_006522578.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5189685.2559.1, SRR5189685.29571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGTGG AAAACATGGC GCACTGTGAG 
TTTCTTCTCC TGAGAGATCT GCTCATCCGC TCCCATCTCC AGGACCTGAA GGACATCACC 
CACAATGTAC ACTACGAGAA CTACCGGGTA CTCAGACTCA ATGAGAGCCA CGTGCTGCCC 
CGGGGGCCAG GCTGGGTGAA CCTGGCTCCA GTCTCCCCTG GACAGCTGAT GGCCCCTGGA 
CCGGGAAAGG TCCGAAAGCG AGCAAACAAG GATACCAAGG ACGAAGAGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360874.1
CDS	100..1173
Translation	MDERRSPSPCSSRPSSPRTPPNEMFGPVGIEAVLDQLRIKAMKTGFEFNIMVVGQSGLGKSTMVNTLFKSKVWQSTEPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKTHCIDVYPQKCFDEDVNDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEVENMAHCEFLLLRDLLIRSHLQDLKDITHNVHYENYRVLRLNESHVLPRGPGWVNLAPVSPGQLMAPGPGKVRKRANKDTKDEEC

Target ORF information:

RefSeq Version	NM_001373945.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus septin 12 (Septin12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373945.1

ORF Insert Sequence:

ATGGATGAGA GGCGGTCGCC CTCCCCCTGC TCCTCAAGGC CCTCCAGCCC CAGGACCCCA 
CCCAATGAGA TGTTTGGTCC TGTGGGCATT GAGGCTGTGC TAGACCAGCT GAGGATCAAG 
GCTATGAAGA CTGGGTTTGA GTTCAATATC ATGGTAGTAG GGCAGAGTGG GCTAGGCAAG 
TCCACCATGG TGAACACACT GTTCAAGTCC AAAGTATGGC AGTCAACTGA ACCCAACCTG 
GATGTACCCA TGCCACAAAC ATTGGAGCTG CACTCTGTGA CCCATGTCAT CGAGGAGAAG 
GGATTGAAGC TGAAGCTGAC AGTGACAGAC ACACCTGGCT TTGGAGACCA GATCAACAAT 
GACAAGTGCT GGGACCCTAT CCTGAGCTAC ATCAACCAAC AGTATGAGCA GTATCTACAA 
GAGGAGCTCC TGATTACCCG CCAGCGCCAC ATCCCCGACA CCCGTGTGCA CTGCTGTGTG 
TACTTTGTGC CACCCACTGG CCACTGCCTA CGGCCCCTAG ACATTGAGTT CCTGAGGCGG 
CTGTGCCGGA CTGTGAATGT GGTGCCGGTG ATCGCCCGGG CTGACAGCCT GACCATTGAA 
GAACGAGATG CCTTCAGGAG CAGGATCCAG CAAAACCTGA AAACTCACTG CATCGATGTG 
TACCCACAGA AGTGTTTCGA CGAGGATGTC AATGACAGAC TTCTCAACAG TAAGATTCGG 
GAGCAGATCC CTTTTGCTGT GGTCGGGGCT GACCGAGAGC ACATAGTGAA TGGGAGATGT 
GTCCTGGGCC GGAAGACCAA ATGGGGCATC ATCGAAGTGG AAAACATGGC GCACTGTGAG 
TTTCTTCTCC TGAGAGATCT GCTCATCCGC TCCCATCTCC AGGACCTGAA GGACATCACC 
CACAATGTAC ACTACGAGAA CTACCGGGTA CTCAGACTCA ATGAGAGCCA CGTGCTGCCC 
CGGGGGCCAG GCTGGGTGAA CCTGGCTCCA GTCTCCCCTG GACAGCTGAT GGCCCCTGGA 
CCGGGAAAGG TCCGAAAGCG AGCAAACAAG GATACCAAGG ACGAAGAGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.
PLoS genetics13(3)e1006631(2017 Mar)
Shen YR,Wang HY,Kuo YC,Shih SC,Hsu CH,Chen YR,Wu SR,Wang CY,Kuo PL

SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.
International journal of molecular sciences17(11)(2016 Nov)
Lai TH,Wu YY,Wang YY,Chen MF,Wang P,Chen TM,Wu YN,Chiang HS,Kuo PL,Lin YH

SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.
Journal of cell science128(5)923-34(2015 Mar)
Kuo YC,Shen YR,Chen HI,Lin YH,Wang YY,Chen YR,Wang CY,Kuo PL

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Sept12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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