Itprid2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itprid2
Entrez Gene ID	70599
Full Name	sperm specific antigen 2
Synonyms	5730488C15Rik,CS-1,CS1,KRAP,SPAG13,mKIAA1927
General protein information	Preferred Names sperm specific antigen 2 Names sperm-specific antigen 2 homolog ki-ras-induced actin-interacting protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_080558.4	NP_542125.3	protein ITPRID2
XM_006500165.3	XP_006500228.1	protein ITPRID2 isoform X1

Pathways from BioSystems

Homologies

	SSFA2	XP_001101290.2
Xenopus tropicalis (tropical clawed frog)	ssfa2	NP_001072601.2
Homo sapiens (human)	SSFA2	NP_001123917.1
Canis lupus familiaris (dog)	SSFA2	XP_005640468.1
Rattus norvegicus (Norway rat)	Ssfa2	NP_001101208.1
Mus musculus (house mouse)	Ssfa2	NP_542125.3
Bos taurus (cattle)	SSFA2	NP_001179026.1
Pan troglodytes (chimpanzee)	SSFA2	XP_003309397.1
Gallus gallus (chicken)	SSFA2	XP_004942747.1
Danio rerio (zebrafish)	ssfa2	XP_001918979.4

Gene Ontology
Bibliography

Related articles in PubMed

Identification of KRAP-expressing cells and the functional relevance of KRAP to the subcellular localization of IP3R in the stomach and kidney.
Fujimoto T, Shirasawa S
International journal of molecular medicine30(6)1287-93(2012 Dec)

KRAS-induced actin-interacting protein: a potent target for obesity, diabetes and cancer.
Fujimoto T, Shirasawa S
Anticancer research31(7)2413-7(2011 Jul)

KRAS-induced actin-interacting protein regulates inositol 1,4,5-trisphosphate-receptor-mediated calcium release.
Fujimoto T, Machida T, Tsunoda T, Doi K, Ota T, Kuroki M, Shirasawa S
Biochemical and biophysical research communications408(2)214-7(2011 May)

KRAS-induced actin-interacting protein is required for the proper localization of inositol 1,4,5-trisphosphate receptor in the epithelial cells.
Fujimoto T, Machida T, Tanaka Y, Tsunoda T, Doi K, Ota T, Okamura T, Kuroki M, Shirasawa S
Biochemical and biophysical research communications407(2)438-43(2011 Apr)

Pancreatic hypertrophy in Ki-ras-induced actin-interacting protein gene knockout mice.
Miyasaka K, Fujimoto T, Kawanami T, Takiguchi S, Jimi A, Funakoshi A, Shirasawa S
Pancreas40(1)79-83(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KRAP physically associates with IP3 receptors and regulates their proper localization in the mucous cells and the chief cells of the stomach and in the proximal tubular cells of the kidneys.
Title: Identification of KRAP-expressing cells and the functional relevance of KRAP to the subcellular localization of IP3R in the stomach and kidney.

KRAP-deficient mice show altered whole-body energy metabolism & resistance to diet-induced obesity and diabetes. Review.
Title: KRAS-induced actin-interacting protein: a potent target for obesity, diabetes and cancer.

KRAP is involved in the proper regulation of IP3R-mediated Ca2+ release.
Title: KRAS-induced actin-interacting protein regulates inositol 1,4,5-trisphosphate-receptor-mediated calcium release.

These findings demonstrate that KRAP physically associates with IP(3)R and regulates the proper localization of IP(3)R in the epithelial cells in vivo and cultured cells.
Title: KRAS-induced actin-interacting protein is required for the proper localization of inositol 1,4,5-trisphosphate receptor in the epithelial cells.

The lack and/or decrease in Ki-ras-induced actin-interacting protein level in the pancreas may promote the pancreatic growth and hypertrophy.
Title: Pancreatic hypertrophy in Ki-ras-induced actin-interacting protein gene knockout mice.

The following Itprid2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itprid2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu30877	XM_006500165.3 Latest version!	Mus musculus ITPR interacting domain containing 2 (Itprid2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09708	NM_080558.4 Latest version!	Mus musculus ITPR interacting domain containing 2 (Itprid2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itprid2 ( XM_006500165.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500228.1 Mus musculus ITPR interacting domain containing 2 (Itprid2), transcript variant X2, mRNA.

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CloneID	OMu30877
Clone ID Related Accession (Same CDS sequence)	XM_006500165.3
Accession Version	XM_006500165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein ITPRID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTG AAGATCATCT GTTGAGAACT GCAAGTCAGC ATTCCGATAG CAGCGGCTTT 
GCTGAGGACT CCACAGACTG TGTCTCCCTC AACCACCTCC TGGTGAACGA GTCCCTGCAA 
GCCATGGGGA GCAGTGCTGA CAGCTGTGAC AGTGAAACCA CCGTCACATC GCTTGGTGAG 
GACCACGTGA CTCCTACAGC GCAAGACCAG CCTTACTTCA ATGAGTCAGA GGAGGAGTCC 
CTGGCTCCTC TGCAGAAAGG AAGAGCAAAG GTGGAAATAG TGGCTGAGAA AAGGAAAGCC 
GACAACCAAG ATTTCCCTCA GTGTGTGACC GCAGAGAATG CTGGAAACAA TGAGTCCACA 
AAGGGCCCGT GTGAGCCTGG TCATCAGATC ACAGAAACAG GGGAGCATCC ACCTCTGGCA 
GCCACTGGAG AGCTCCCCAG GGAAGAGAGT GTCGAGAGTG ATGTAGAGAA AGGCAGTGAA 
TGTGAATTTG CCCAGTACAC CACACACCAT ATTCTCAGAT CCTTGGCTTC CTTTGAAGCG 
CAGGGCAGCG GTATGAGCTC TGAAAAGAAA ACTGGGTTTC CCTCTTCTGT GGACAGAGTG 
AACACTGCCC TGCAGAGAGC TCAAATGAAG GTCTGCAGTA TGTCTGGGCA GAGAGTAGGG 
CGTAGCCTAA TAAAATCAAA GGATCTGTTG AAACAAAGGT ACTTGCTCGC AAAAGCTGGC 
TATCCTCTGA GAAGGTCTCA GTCTTTGCCC ACCACTTTAC TGAGCCCAGT ACGGGTGGTG 
TCTTCTGTCA ATGTGCGATT ATCTCCAGGA AAAGAGACCA GGTGCAGCCC ACCGTCCTTC 
ACCTACAAGT ACACACCCGA AGAGGAACAA GACCTGGAAA AGCAAGGAAC TGAACACGAT 
GGTCAGTCTC TGGTTAAATC TACCATCTTC ATCCCGCCAC CCTCTGTGAA GAAAGAAGAA 
GCCCCTCAGA GTGAGGGGAC ACGACTGGAG GAATGCCACC ATGGAAGGCT TGCCCCCTGC 
CCACAGTTCG CTCCAATATC CCAGTCCACC TGCTCCCTTC ACTCTGTCCA CTCTGAGTGG 
CAGGACAGAC CCCTGTGTGA ACACATGAGG ACTCTGAGTG CCCACAGTGT CCCCAACATA 
TCGGGTGCCG CATGTAGTGC CTTCTCTCCT TTCGGGTGTC CCTATTCACA CAGACATGCT 
GCCCACCCAT ACAGGGCATG CTCTGTGAAT CCTCCTTCTG CCATTGAGAT GCAGTTGCGG 
AGAGTGTTGC ATGATATTAG AAGCTCACTA CAGAATCTTT CACAGTATCC TATGACGAGA 
GGACCTGACC TTGCTGCTGC TCCATACAGT ACTCAGAACT CGTCTGTCCT ACCTCTTTAT 
GAAAATACCT TCCAGGAGCT TCAAGTCGTG AGGCGGAGCC TGAATCTGTT CAGAACGCAG 
ATGATGGACT TGGAGCTGGC CATGCTGCGG CAGCAAACCG TGGTGTACCC TCATATGACA 
GAGGAGGACA GGTATGAAGT TGATCAGCTG CAGGGTTTGC GGAACTCTGT CCGAATGGAA 
CTGCAGGACC TGGAGATGCA GCTGGAGGAG CGCTTGCTGG GCCTGGATGA GCAGCTCCGT 
GCAGTGCGGG TGCCATCACC TTTCCGCCCC TCTGCACTCC CGGGGATGTG TGGCAGCAGG 
AGCGTAGATA ACTTATCATG CCCGTCTCCA CTGAACGTCA TGGAGCCCGT CACAGAGCTA 
ATTCGGGAGC AATCATACCT GAAGTCTGAG CTGGGCCTGG GCCTTGGAGA CATGGCGTAT 
GAAATTCCTC CTGGAGAGAG CTCGGAGTCC GTTTTCTCTC AAGCCACATC AGAGTCATCT 
TCCGTCTGCT CCAGCCCCTC CCACACCAAC AGAAGATCTA GAGGGCTACC TGGGAGCAAA 
CCCAGAGCCC GCTTAGTGGC GAGAAAGAAG ATATTCCGAG CCTCTGTGGC CCTGACACCA 
ACTGCCCCCT CTAGAACAGG CTCTGTGCAA ACACCTCCAG ATCTGGAGAG TTCCGAAGAA 
GCTGGAGGAG CTGAGGAAGC ATCCCCGGTA GTGGGGCTTG CATCTCACGT GGAGGAAGAG 
CCAGAGGATC TCTCACTGAT GCCAGCAGCC GAGGAGATGC ACAGGAATGT GGAGCAAGAT 
GAACTGCAGC AAGTCATCCG AGAGATTAAA GAGTCTATTG TTGGGGAAAT TCGACGAGAA 
ATTGTAAGTG GACTTTTGGC AGCAGTGTCT TCAAGTAAAG CACCTGGTCC TAAGCAGGAC 
AGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500228.1
CDS	82..2370
Translation	MTTEDHLLRTASQHSDSSGFAEDSTDCVSLNHLLVNESLQAMGSSADSCDSETTVTSLGEDHVTPTAQDQPYFNESEEESLAPLQKGRAKVEIVAEKRKADNQDFPQCVTAENAGNNESTKGPCEPGHQITETGEHPPLAATGELPREESVESDVEKGSECEFAQYTTHHILRSLASFEAQGSGMSSEKKTGFPSSVDRVNTALQRAQMKVCSMSGQRVGRSLIKSKDLLKQRYLLAKAGYPLRRSQSLPTTLLSPVRVVSSVNVRLSPGKETRCSPPSFTYKYTPEEEQDLEKQGTEHDGQSLVKSTIFIPPPSVKKEEAPQSEGTRLEECHHGRLAPCPQFAPISQSTCSLHSVHSEWQDRPLCEHMRTLSAHSVPNISGAACSAFSPFGCPYSHRHAAHPYRACSVNPPSAIEMQLRRVLHDIRSSLQNLSQYPMTRGPDLAAAPYSTQNSSVLPLYENTFQELQVVRRSLNLFRTQMMDLELAMLRQQTVVYPHMTEEDRYEVDQLQGLRNSVRMELQDLEMQLEERLLGLDEQLRAVRVPSPFRPSALPGMCGSRSVDNLSCPSPLNVMEPVTELIREQSYLKSELGLGLGDMAYEIPPGESSESVFSQATSESSSVCSSPSHTNRRSRGLPGSKPRARLVARKKIFRASVALTPTAPSRTGSVQTPPDLESSEEAGGAEEASPVVGLASHVEEEPEDLSLMPAAEEMHRNVEQDELQQVIREIKESIVGEIRREIVSGLLAAVSSSKAPGPKQDSH

Target ORF information:

RefSeq Version	XM_006500165.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ITPR interacting domain containing 2 (Itprid2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500165.3

ORF Insert Sequence:

ATGACCACTG AAGATCATCT GTTGAGAACT GCAAGTCAGC ATTCCGATAG CAGCGGCTTT 
GCTGAGGACT CCACAGACTG TGTCTCCCTC AACCACCTCC TGGTGAACGA GTCCCTGCAA 
GCCATGGGGA GCAGTGCTGA CAGCTGTGAC AGTGAAACCA CCGTCACATC GCTTGGTGAG 
GACCACGTGA CTCCTACAGC GCAAGACCAG CCTTACTTCA ATGAGTCAGA GGAGGAGTCC 
CTGGCTCCTC TGCAGAAAGG AAGAGCAAAG GTGGAAATAG TGGCTGAGAA AAGGAAAGCC 
GACAACCAAG ATTTCCCTCA GTGTGTGACC GCAGAGAATG CTGGAAACAA TGAGTCCACA 
AAGGGCCCGT GTGAGCCTGG TCATCAGATC ACAGAAACAG GGGAGCATCC ACCTCTGGCA 
GCCACTGGAG AGCTCCCCAG GGAAGAGAGT GTCGAGAGTG ATGTAGAGAA AGGCAGTGAA 
TGTGAATTTG CCCAGTACAC CACACACCAT ATTCTCAGAT CCTTGGCTTC CTTTGAAGCG 
CAGGGCAGCG GTATGAGCTC TGAAAAGAAA ACTGGGTTTC CCTCTTCTGT GGACAGAGTG 
AACACTGCCC TGCAGAGAGC TCAAATGAAG GTCTGCAGTA TGTCTGGGCA GAGAGTAGGG 
CGTAGCCTAA TAAAATCAAA GGATCTGTTG AAACAAAGGT ACTTGCTCGC AAAAGCTGGC 
TATCCTCTGA GAAGGTCTCA GTCTTTGCCC ACCACTTTAC TGAGCCCAGT ACGGGTGGTG 
TCTTCTGTCA ATGTGCGATT ATCTCCAGGA AAAGAGACCA GGTGCAGCCC ACCGTCCTTC 
ACCTACAAGT ACACACCCGA AGAGGAACAA GACCTGGAAA AGCAAGGAAC TGAACACGAT 
GGTCAGTCTC TGGTTAAATC TACCATCTTC ATCCCGCCAC CCTCTGTGAA GAAAGAAGAA 
GCCCCTCAGA GTGAGGGGAC ACGACTGGAG GAATGCCACC ATGGAAGGCT TGCCCCCTGC 
CCACAGTTCG CTCCAATATC CCAGTCCACC TGCTCCCTTC ACTCTGTCCA CTCTGAGTGG 
CAGGACAGAC CCCTGTGTGA ACACATGAGG ACTCTGAGTG CCCACAGTGT CCCCAACATA 
TCGGGTGCCG CATGTAGTGC CTTCTCTCCT TTCGGGTGTC CCTATTCACA CAGACATGCT 
GCCCACCCAT ACAGGGCATG CTCTGTGAAT CCTCCTTCTG CCATTGAGAT GCAGTTGCGG 
AGAGTGTTGC ATGATATTAG AAGCTCACTA CAGAATCTTT CACAGTATCC TATGACGAGA 
GGACCTGACC TTGCTGCTGC TCCATACAGT ACTCAGAACT CGTCTGTCCT ACCTCTTTAT 
GAAAATACCT TCCAGGAGCT TCAAGTCGTG AGGCGGAGCC TGAATCTGTT CAGAACGCAG 
ATGATGGACT TGGAGCTGGC CATGCTGCGG CAGCAAACCG TGGTGTACCC TCATATGACA 
GAGGAGGACA GGTATGAAGT TGATCAGCTG CAGGGTTTGC GGAACTCTGT CCGAATGGAA 
CTGCAGGACC TGGAGATGCA GCTGGAGGAG CGCTTGCTGG GCCTGGATGA GCAGCTCCGT 
GCAGTGCGGG TGCCATCACC TTTCCGCCCC TCTGCACTCC CGGGGATGTG TGGCAGCAGG 
AGCGTAGATA ACTTATCATG CCCGTCTCCA CTGAACGTCA TGGAGCCCGT CACAGAGCTA 
ATTCGGGAGC AATCATACCT GAAGTCTGAG CTGGGCCTGG GCCTTGGAGA CATGGCGTAT 
GAAATTCCTC CTGGAGAGAG CTCGGAGTCC GTTTTCTCTC AAGCCACATC AGAGTCATCT 
TCCGTCTGCT CCAGCCCCTC CCACACCAAC AGAAGATCTA GAGGGCTACC TGGGAGCAAA 
CCCAGAGCCC GCTTAGTGGC GAGAAAGAAG ATATTCCGAG CCTCTGTGGC CCTGACACCA 
ACTGCCCCCT CTAGAACAGG CTCTGTGCAA ACACCTCCAG ATCTGGAGAG TTCCGAAGAA 
GCTGGAGGAG CTGAGGAAGC ATCCCCGGTA GTGGGGCTTG CATCTCACGT GGAGGAAGAG 
CCAGAGGATC TCTCACTGAT GCCAGCAGCC GAGGAGATGC ACAGGAATGT GGAGCAAGAT 
GAACTGCAGC AAGTCATCCG AGAGATTAAA GAGTCTATTG TTGGGGAAAT TCGACGAGAA 
ATTGTAAGTG GACTTTTGGC AGCAGTGTCT TCAAGTAAAG CACCTGGTCC TAAGCAGGAC 
AGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itprid2 ( NM_080558.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_542125.3 Mus musculus ITPR interacting domain containing 2 (Itprid2), mRNA.

Price & Availability↑

CloneID	OMu09708
Clone ID Related Accession (Same CDS sequence)	NM_080558.4
Accession Version	NM_080558.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	protein ITPRID2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ129385.1, CF539296.1 and BC079535.1. On Jul 26, 2008 this sequence version replaced NM_080558.3. ##Evidence-Data-START## Transcript exon combination :: AB093303.1, SRR1660821.191001.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCGAC CCCTGTCGGC GGAGGCGGAG GAGGAACTGG AGTGGCAAGT GGCGAGTCGC 
AGGAGGAAGG CCTGGGCCAA GTGCCGCAGC TCCTGGCAGG CGTCGGAGAC CGAGGATCTG 
TCCACAGAGA CGACGACGCA GGACGAGGAC GAGGACGACG AGGAGGACCT CCCAGGCACG 
AAGCTGCCGG CACCCGCGGG GCGAGGAAAC GTGCCCAACG AGAAGATCGC GATATGGCTC 
AAAGACTGCC GCACCCCTCT GGGAGCCTCA CTGGATGAGC AAAGCAGTGG TACGCCGAAG 
GGTGTGCTTG TGAGAAATGG AGGAAGCTTT GAAGATGACC TGTCACTGGG AGCTGAAGCC 
AATCACCTAC ATGAACCTGA TGCTCAAGTT GAAAACTGCA ACAATATCTT GGCCAAGGAG 
AGAAGACTAC AGTTTCATCA GAAAGGGAGA AGTATGAATT CCACTGGGTC TGGGAAAAGC 
AGTGGTACCG TGTCAAGTGT CTCAGAACTG CTGGAGCTTT ACGAGGAAGA TCCTGAAGAA 
ATTCTATATA ATCTTGGATT TGGACGAGAT GAACCAGATA TTGCTTCTAA AATTCCTTCC 
AGATTTTTTA ATTCATCATC CTTTGCCAGA GGCATAGACA TTAAAGTATT TCTGAGTGCA 
CAGATGCAAC GGATGGAAGT AGAGAACCCA AACTATGCTC TGACAAGCCG ATTTCGCCAA 
ATTGAAGTCC TGACTACTGT GGCCAATGCA TTTTCTTCTC TATATTCCCA AGTCTCTGGA 
ACTCCACTGC AGAGGATTGG AAGTATGTCC TCGGTGACCT CCACCAAGGA GGTGGCAGAC 
TCCCCGCCCC CTCTAACTCG AAGCAACACT GCAAACCGTC TGATGAAAAC ACTATCAAAA 
CTGAACTTAT GTGTTGATAA AACAGAGAAA GGAGAAGGGG GCTCTTCCCC CGCCACTGAG 
AAAGGAAGGA CTCTAAGCAT CTCACTGTCT GAAGATGGCG GCGGCGGCAA GAGTGACCCT 
AAGCTTCAGA AAGTTGTAAA GAAGAAGGAG TCGTCTTCGA TGCTGGCTAC GGTTACAGAA 
GAAGTCTCAG GTAGTTCATC AACCGTCACA GACAGCGTTG ATGCAGACAG ACTTTCCGAG 
GAAGCAGACA GTACCATTAG CCACCAGGAG GAAAGTGAAG AAAGCAGAGA GGCTCACAGT 
CAGGAGAAGG ACCCGCTCCG CAAGTCTGCT GTGACAGATC CTGACTTGGG TCATGATGGC 
CGCGTGTCCA GCCACTGCGA GCTGGAGAGC AGCAGTGAGC TGAAAAGTGC CCAGGCGTCT 
TCGAGCGAGA AAGAGCCTTG TGCACCCCTG ACAATCCCAT CCATACGGAA CATAATGACG 
CAGCAGAAAG ACTCCTTTGA GATGGAAGAG GTCCAGAGCA CAGAGGGAGA AGCCCCTCAC 
GTGCCAGCCA CTTGCCAGCT AAGTCTTGCC AAGTCAAAAA GAGATCATCT GTTGAGAACT 
GCAAGTCAGC ATTCCGATAG CAGCGGCTTT GCTGAGGACT CCACAGACTG TGTCTCCCTC 
AACCACCTCC TGGTGAACGA GTCCCTGCAA GCCATGGGGA GCAGTGCTGA CAGCTGTGAC 
AGTGAAACCA CCGTCACATC GCTTGGTGAG GACCACGTGA CTCCTACAGC GCAAGACCAG 
CCTTACTTCA ATGAGTCAGA GGAGGAGTCC CTGGCTCCTC TGCAGAAAGG AAGAGCAAAG 
GTGGAAATAG TGGCTGAGAA AAGGAAAGCC GACAACCAAG ATTTCCCTCA GTGTGTGACC 
GCAGAGAATG CTGGAAACAA TGAGTCCACA AAGGGCCCGT GTGAGCCTGG TCATCAGATC 
ACAGAAACAG GGGAGCATCC ACCTCTGGCA GCCACTGGAG AGCTCCCCAG GGAAGAGAGT 
GTCGAGAGTG ATGTAGAGAA AGGCAGTGAA TGTGAATTTG CCCAGTACAC CACACACCAT 
ATTCTCAGAT CCTTGGCTTC CTTTGAAGCG CAGGGCAGCG GTATGAGCTC TGAAAAGAAA 
ACTGGGTTTC CCTCTTCTGT GGACAGAGTG AACACTGCCC TGCAGAGAGC TCAAATGAAG 
GTCTGCAGTA TGTCTGGGCA GAGAGTAGGG CGTAGCCTAA TAAAATCAAA GGATCTGTTG 
AAACAAAGGT ACTTGCTCGC AAAAGCTGGC TATCCTCTGA GAAGGTCTCA GTCTTTGCCC 
ACCACTTTAC TGAGCCCAGT ACGGGTGGTG TCTTCTGTCA ATGTGCGATT ATCTCCAGGA 
AAAGAGACCA GGTGCAGCCC ACCGTCCTTC ACCTACAAGT ACACACCCGA AGAGGAACAA 
GACCTGGAAA AGCAAGGAAC TGAACACGAT GGTCAGTCTC TGGTTAAATC TACCATCTTC 
ATCCCGCCAC CCTCTGTGAA GAAAGAAGAA GCCCCTCAGA GTGAGGGGAC ACGACTGGAG 
GAATGCCACC ATGGAAGGCT TGCCCCCTGC CCACAGTTCG CTCCAATATC CCAGTCCACC 
TGCTCCCTTC ACTCTGTCCA CTCTGAGTGG CAGGACAGAC CCCTGTGTGA ACACATGAGG 
ACTCTGAGTG CCCACAGTGT CCCCAACATA TCGGGTGCCG CATGTAGTGC CTTCTCTCCT 
TTCGGGTGTC CCTATTCACA CAGACATGCT GCCCACCCAT ACAGGGCATG CTCTGTGAAT 
CCTCCTTCTG CCATTGAGAT GCAGTTGCGG AGAGTGTTGC ATGATATTAG AAGCTCACTA 
CAGAATCTTT CACAGTATCC TATGACGAGA GGACCTGACC TTGCTGCTGC TCCATACAGT 
ACTCAGAACT CGTCTGTCCT ACCTCTTTAT GAAAATACCT TCCAGGAGCT TCAAGTCGTG 
AGGCGGAGCC TGAATCTGTT CAGAACGCAG ATGATGGACT TGGAGCTGGC CATGCTGCGG 
CAGCAAACCG TGGTGTACCC TCATATGACA GAGGAGGACA GGTATGAAGT TGATCAGCTG 
CAGGGTTTGC GGAACTCTGT CCGAATGGAA CTGCAGGACC TGGAGATGCA GCTGGAGGAG 
CGCTTGCTGG GCCTGGATGA GCAGCTCCGT GCAGTGCGGG TGCCATCACC TTTCCGCCCC 
TCTGCACTCC CGGGGATGTG TGGCAGCAGG AGCGTAGATA ACTTATCATG CCCGTCTCCA 
CTGAACGTCA TGGAGCCCGT CACAGAGCTA ATTCGGGAGC AATCATACCT GAAGTCTGAG 
CTGGGCCTGG GCCTTGGAGA CATGGCGTAT GAAATTCCTC CTGGAGAGAG CTCGGAGTCC 
GTTTTCTCTC AAGCCACATC AGAGTCATCT TCCGTCTGCT CCAGCCCCTC CCACACCAAC 
AGAAGATCTA GAGGGCTACC TGGGAGCAAA CCCAGAGCCC GCTTAGTGGC GAGAAAGAAG 
ATATTCCGAG CCTCTGTGGC CCTGACACCA ACTGCCCCCT CTAGAACAGG CTCTGTGCAA 
ACACCTCCAG ATCTGGAGAG TTCCGAAGAA GCTGGAGGAG CTGAGGAAGC ATCCCCGGTA 
GTGGGGCTTG CATCTCACGT GGAGGAAGAG CCAGAGGATC TCTCACTGAT GCCAGCAGCC 
GAGGAGATGC ACAGGAATGT GGAGCAAGAT GAACTGCAGC AAGTCATCCG AGAGATTAAA 
GAGTCTATTG TTGGGGAAAT TCGACGAGAA ATTGTAAGTG GACTTTTGGC AGCAGTGTCT 
TCAAGTAAAG CACCTGGTCC TAAGCAGGAC AGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542125.3
CDS	116..3874
Translation	MNRPLSAEAEEELEWQVASRRRKAWAKCRSSWQASETEDLSTETTTQDEDEDDEEDLPGTKLPAPAGRGNVPNEKIAIWLKDCRTPLGASLDEQSSGTPKGVLVRNGGSFEDDLSLGAEANHLHEPDAQVENCNNILAKERRLQFHQKGRSMNSTGSGKSSGTVSSVSELLELYEEDPEEILYNLGFGRDEPDIASKIPSRFFNSSSFARGIDIKVFLSAQMQRMEVENPNYALTSRFRQIEVLTTVANAFSSLYSQVSGTPLQRIGSMSSVTSTKEVADSPPPLTRSNTANRLMKTLSKLNLCVDKTEKGEGGSSPATEKGRTLSISLSEDGGGGKSDPKLQKVVKKKESSSMLATVTEEVSGSSSTVTDSVDADRLSEEADSTISHQEESEESREAHSQEKDPLRKSAVTDPDLGHDGRVSSHCELESSSELKSAQASSSEKEPCAPLTIPSIRNIMTQQKDSFEMEEVQSTEGEAPHVPATCQLSLAKSKRDHLLRTASQHSDSSGFAEDSTDCVSLNHLLVNESLQAMGSSADSCDSETTVTSLGEDHVTPTAQDQPYFNESEEESLAPLQKGRAKVEIVAEKRKADNQDFPQCVTAENAGNNESTKGPCEPGHQITETGEHPPLAATGELPREESVESDVEKGSECEFAQYTTHHILRSLASFEAQGSGMSSEKKTGFPSSVDRVNTALQRAQMKVCSMSGQRVGRSLIKSKDLLKQRYLLAKAGYPLRRSQSLPTTLLSPVRVVSSVNVRLSPGKETRCSPPSFTYKYTPEEEQDLEKQGTEHDGQSLVKSTIFIPPPSVKKEEAPQSEGTRLEECHHGRLAPCPQFAPISQSTCSLHSVHSEWQDRPLCEHMRTLSAHSVPNISGAACSAFSPFGCPYSHRHAAHPYRACSVNPPSAIEMQLRRVLHDIRSSLQNLSQYPMTRGPDLAAAPYSTQNSSVLPLYENTFQELQVVRRSLNLFRTQMMDLELAMLRQQTVVYPHMTEEDRYEVDQLQGLRNSVRMELQDLEMQLEERLLGLDEQLRAVRVPSPFRPSALPGMCGSRSVDNLSCPSPLNVMEPVTELIREQSYLKSELGLGLGDMAYEIPPGESSESVFSQATSESSSVCSSPSHTNRRSRGLPGSKPRARLVARKKIFRASVALTPTAPSRTGSVQTPPDLESSEEAGGAEEASPVVGLASHVEEEPEDLSLMPAAEEMHRNVEQDELQQVIREIKESIVGEIRREIVSGLLAAVSSSKAPGPKQDSH

Target ORF information:

RefSeq Version	NM_080558.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ITPR interacting domain containing 2 (Itprid2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080558.4

ORF Insert Sequence:

ATGAACCGAC CCCTGTCGGC GGAGGCGGAG GAGGAACTGG AGTGGCAAGT GGCGAGTCGC 
AGGAGGAAGG CCTGGGCCAA GTGCCGCAGC TCCTGGCAGG CGTCGGAGAC CGAGGATCTG 
TCCACAGAGA CGACGACGCA GGACGAGGAC GAGGACGACG AGGAGGACCT CCCAGGCACG 
AAGCTGCCGG CACCCGCGGG GCGAGGAAAC GTGCCCAACG AGAAGATCGC GATATGGCTC 
AAAGACTGCC GCACCCCTCT GGGAGCCTCA CTGGATGAGC AAAGCAGTGG TACGCCGAAG 
GGTGTGCTTG TGAGAAATGG AGGAAGCTTT GAAGATGACC TGTCACTGGG AGCTGAAGCC 
AATCACCTAC ATGAACCTGA TGCTCAAGTT GAAAACTGCA ACAATATCTT GGCCAAGGAG 
AGAAGACTAC AGTTTCATCA GAAAGGGAGA AGTATGAATT CCACTGGGTC TGGGAAAAGC 
AGTGGTACCG TGTCAAGTGT CTCAGAACTG CTGGAGCTTT ACGAGGAAGA TCCTGAAGAA 
ATTCTATATA ATCTTGGATT TGGACGAGAT GAACCAGATA TTGCTTCTAA AATTCCTTCC 
AGATTTTTTA ATTCATCATC CTTTGCCAGA GGCATAGACA TTAAAGTATT TCTGAGTGCA 
CAGATGCAAC GGATGGAAGT AGAGAACCCA AACTATGCTC TGACAAGCCG ATTTCGCCAA 
ATTGAAGTCC TGACTACTGT GGCCAATGCA TTTTCTTCTC TATATTCCCA AGTCTCTGGA 
ACTCCACTGC AGAGGATTGG AAGTATGTCC TCGGTGACCT CCACCAAGGA GGTGGCAGAC 
TCCCCGCCCC CTCTAACTCG AAGCAACACT GCAAACCGTC TGATGAAAAC ACTATCAAAA 
CTGAACTTAT GTGTTGATAA AACAGAGAAA GGAGAAGGGG GCTCTTCCCC CGCCACTGAG 
AAAGGAAGGA CTCTAAGCAT CTCACTGTCT GAAGATGGCG GCGGCGGCAA GAGTGACCCT 
AAGCTTCAGA AAGTTGTAAA GAAGAAGGAG TCGTCTTCGA TGCTGGCTAC GGTTACAGAA 
GAAGTCTCAG GTAGTTCATC AACCGTCACA GACAGCGTTG ATGCAGACAG ACTTTCCGAG 
GAAGCAGACA GTACCATTAG CCACCAGGAG GAAAGTGAAG AAAGCAGAGA GGCTCACAGT 
CAGGAGAAGG ACCCGCTCCG CAAGTCTGCT GTGACAGATC CTGACTTGGG TCATGATGGC 
CGCGTGTCCA GCCACTGCGA GCTGGAGAGC AGCAGTGAGC TGAAAAGTGC CCAGGCGTCT 
TCGAGCGAGA AAGAGCCTTG TGCACCCCTG ACAATCCCAT CCATACGGAA CATAATGACG 
CAGCAGAAAG ACTCCTTTGA GATGGAAGAG GTCCAGAGCA CAGAGGGAGA AGCCCCTCAC 
GTGCCAGCCA CTTGCCAGCT AAGTCTTGCC AAGTCAAAAA GAGATCATCT GTTGAGAACT 
GCAAGTCAGC ATTCCGATAG CAGCGGCTTT GCTGAGGACT CCACAGACTG TGTCTCCCTC 
AACCACCTCC TGGTGAACGA GTCCCTGCAA GCCATGGGGA GCAGTGCTGA CAGCTGTGAC 
AGTGAAACCA CCGTCACATC GCTTGGTGAG GACCACGTGA CTCCTACAGC GCAAGACCAG 
CCTTACTTCA ATGAGTCAGA GGAGGAGTCC CTGGCTCCTC TGCAGAAAGG AAGAGCAAAG 
GTGGAAATAG TGGCTGAGAA AAGGAAAGCC GACAACCAAG ATTTCCCTCA GTGTGTGACC 
GCAGAGAATG CTGGAAACAA TGAGTCCACA AAGGGCCCGT GTGAGCCTGG TCATCAGATC 
ACAGAAACAG GGGAGCATCC ACCTCTGGCA GCCACTGGAG AGCTCCCCAG GGAAGAGAGT 
GTCGAGAGTG ATGTAGAGAA AGGCAGTGAA TGTGAATTTG CCCAGTACAC CACACACCAT 
ATTCTCAGAT CCTTGGCTTC CTTTGAAGCG CAGGGCAGCG GTATGAGCTC TGAAAAGAAA 
ACTGGGTTTC CCTCTTCTGT GGACAGAGTG AACACTGCCC TGCAGAGAGC TCAAATGAAG 
GTCTGCAGTA TGTCTGGGCA GAGAGTAGGG CGTAGCCTAA TAAAATCAAA GGATCTGTTG 
AAACAAAGGT ACTTGCTCGC AAAAGCTGGC TATCCTCTGA GAAGGTCTCA GTCTTTGCCC 
ACCACTTTAC TGAGCCCAGT ACGGGTGGTG TCTTCTGTCA ATGTGCGATT ATCTCCAGGA 
AAAGAGACCA GGTGCAGCCC ACCGTCCTTC ACCTACAAGT ACACACCCGA AGAGGAACAA 
GACCTGGAAA AGCAAGGAAC TGAACACGAT GGTCAGTCTC TGGTTAAATC TACCATCTTC 
ATCCCGCCAC CCTCTGTGAA GAAAGAAGAA GCCCCTCAGA GTGAGGGGAC ACGACTGGAG 
GAATGCCACC ATGGAAGGCT TGCCCCCTGC CCACAGTTCG CTCCAATATC CCAGTCCACC 
TGCTCCCTTC ACTCTGTCCA CTCTGAGTGG CAGGACAGAC CCCTGTGTGA ACACATGAGG 
ACTCTGAGTG CCCACAGTGT CCCCAACATA TCGGGTGCCG CATGTAGTGC CTTCTCTCCT 
TTCGGGTGTC CCTATTCACA CAGACATGCT GCCCACCCAT ACAGGGCATG CTCTGTGAAT 
CCTCCTTCTG CCATTGAGAT GCAGTTGCGG AGAGTGTTGC ATGATATTAG AAGCTCACTA 
CAGAATCTTT CACAGTATCC TATGACGAGA GGACCTGACC TTGCTGCTGC TCCATACAGT 
ACTCAGAACT CGTCTGTCCT ACCTCTTTAT GAAAATACCT TCCAGGAGCT TCAAGTCGTG 
AGGCGGAGCC TGAATCTGTT CAGAACGCAG ATGATGGACT TGGAGCTGGC CATGCTGCGG 
CAGCAAACCG TGGTGTACCC TCATATGACA GAGGAGGACA GGTATGAAGT TGATCAGCTG 
CAGGGTTTGC GGAACTCTGT CCGAATGGAA CTGCAGGACC TGGAGATGCA GCTGGAGGAG 
CGCTTGCTGG GCCTGGATGA GCAGCTCCGT GCAGTGCGGG TGCCATCACC TTTCCGCCCC 
TCTGCACTCC CGGGGATGTG TGGCAGCAGG AGCGTAGATA ACTTATCATG CCCGTCTCCA 
CTGAACGTCA TGGAGCCCGT CACAGAGCTA ATTCGGGAGC AATCATACCT GAAGTCTGAG 
CTGGGCCTGG GCCTTGGAGA CATGGCGTAT GAAATTCCTC CTGGAGAGAG CTCGGAGTCC 
GTTTTCTCTC AAGCCACATC AGAGTCATCT TCCGTCTGCT CCAGCCCCTC CCACACCAAC 
AGAAGATCTA GAGGGCTACC TGGGAGCAAA CCCAGAGCCC GCTTAGTGGC GAGAAAGAAG 
ATATTCCGAG CCTCTGTGGC CCTGACACCA ACTGCCCCCT CTAGAACAGG CTCTGTGCAA 
ACACCTCCAG ATCTGGAGAG TTCCGAAGAA GCTGGAGGAG CTGAGGAAGC ATCCCCGGTA 
GTGGGGCTTG CATCTCACGT GGAGGAAGAG CCAGAGGATC TCTCACTGAT GCCAGCAGCC 
GAGGAGATGC ACAGGAATGT GGAGCAAGAT GAACTGCAGC AAGTCATCCG AGAGATTAAA 
GAGTCTATTG TTGGGGAAAT TCGACGAGAA ATTGTAAGTG GACTTTTGGC AGCAGTGTCT 
TCAAGTAAAG CACCTGGTCC TAAGCAGGAC AGCCACTGA