Pak4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pak4
Entrez Gene ID	70584
Full Name	p21 protein (Cdc42/Rac)-activated kinase 4
Synonyms	5730488L07Rik,AW555722,mKIAA1142
General protein information	Preferred Names p21 protein (Cdc42/Rac)-activated kinase 4 Names serine/threonine-protein kinase PAK 4 PAK-4 p21-activated kinase 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312296.1	XP_017167785.1	serine/threonine-protein kinase PAK 4 isoform X1
NM_027470.3	NP_081746.1	serine/threonine-protein kinase PAK 4
XM_017312297.1	XP_017167786.1	serine/threonine-protein kinase PAK 4 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PAK4	XP_005616659.1
Gallus gallus (chicken)	PAK4	XP_001233796.3
Homo sapiens (human)	PAK4	NP_001014831.1
Xenopus tropicalis (tropical clawed frog)	pak4	NP_001090671.1
	PAK4	XP_001085295.1
Mus musculus (house mouse)	Pak4	NP_081746.1
Rattus norvegicus (Norway rat)	Pak4	NP_001099708.1
Bos taurus (cattle)	PAK4	NP_001069652.1
Danio rerio (zebrafish)	pak4	NP_001002222.1
Drosophila melanogaster (fruit fly)	mbt	NP_523375.2

Gene Ontology
Bibliography

Related articles in PubMed

PAK4 crystal structures suggest unusual kinase conformational movements.
Zhang EY, Ha BH, Boggon TJ
Biochimica et biophysica acta1866(2)356-365(2018 Feb)

Pdx1-Cre-driven conditional gene depletion suggests PAK4 as dispensable for mouse pancreas development.
Zhao M, Rabieifar P, Costa TDF, Zhuang T, Minden A, L?hr M, Heuchel R, Str?mblad S
Scientific reports7(1)7031(2017 Aug)

Increased Circulating Endothelial Microparticles Associated with PAK4 Play a Key Role in Ventilation-Induced Lung Injury Process.
Pan S, Fei A, Jing L, Zhang X, Gao C
BioMed research international20174902084(2017)

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Brommage R, Liu J, Hansen GM, Kirkpatrick LL, Potter DG, Sands AT, Zambrowicz B, Powell DR, Vogel P
Bone research214034(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PAK4 microparticles may have a role in the ventilation-induced lung injury process
Title: Increased Circulating Endothelial Microparticles Associated with PAK4 Play a Key Role in Ventilation-Induced Lung Injury Process.

These data demonstrate the relevance of PAK4 in osteoclast differentiation and the potential of PAK4 inhibitors for treating osteoclast-related disorders.
Title: Repositioning Potential of PAK4 to Osteoclastic Bone Resorption.

PAK4 promotes alpha-MSH/UVB-induced melanogenesis via the CREB and Wnt/beta-catenin signaling pathways and suggest that PAK4 may be a potential therapeutic target in pigmentation disorders.
Title: p21-activated kinase 4 critically regulates melanogenesis via activation of the CREB/MITF and ?-catenin/MITF pathways.

PAK4 phosphorylates Par6B at Ser143 blocking its interaction with Cdc42.
Title: Functional cross-talk between Cdc42 and two downstream targets, Par6B and PAK4.

Defined here is the overlap in phosphopeptides regulated by K-Ras, Cdc42, and PAK4; perturbation of these signaling components affects phosphoproteins associated with microtubule depolymerization, cytoskeletal organization, and the cell cycle.
Title: Systems-wide analysis of K-Ras, Cdc42, and PAK4 signaling by quantitative phosphoproteomics.

The following Pak4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pak4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14793	NM_027470.3 Latest version!	Mus musculus p21 (RAC1) activated kinase 4 (Pak4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu14793	XM_017312297.1 Latest version!	Mus musculus p21 (RAC1) activated kinase 4 (Pak4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu14793	XM_017312296.1 Latest version!	Mus musculus p21 (RAC1) activated kinase 4 (Pak4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pak4 ( NM_027470.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081746.1 Mus musculus p21 (RAC1) activated kinase 4 (Pak4), mRNA.

Price & Availability↑

CloneID	OMu14793
Clone ID Related Accession (Same CDS sequence)	XM_017312296.1 , NM_027470.3 , XM_017312297.1
Accession Version	NM_027470.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase PAK 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY197606.1, BB839524.1, CF172418.1, BQ572351.1, AC136456.3 and AK017713.1. On Dec 20, 2006 this sequence version replaced NM_027470.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129298.2, AK088512.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGGAA AGAAGAAGAA GCGGGTGGAG ATCTCAGCCC CATCCAACTT CGAGCACCGT 
GTACACACAG GCTTTGATCA GCATGAGCAG AAGTTCACAG GGCTGCCCCG CCAGTGGCAG 
AGCCTGATTG AGGAATCAGC ACGCAGACCC AAGCCCCTGA TCGACCCTGC CTGCATCACG 
TCCATCCAGC CTGGAGCCCC CAAGACCATC GTTCGGGGCA GCAAAGGTGC CAAGGATGGG 
GCTCTCACTC TGCTGCTTGA CGAATTTGAG AACATGTCGG TGACGCGCTC CAACTCCCTA 
CGCAGAGAGA GTCCACCACC ACCTGCCCGT GCCCACCAGG AGAATGGAAT GCTGGAGGAG 
CGGGCAGCCC CGGCCAGAAT GGCCCCTGAC AAAGCTGGAA GCAGAGCCCG GGCCACAGGC 
CACAGTGAGG CAGGCAGTGG CAGTGGTGAC AGACGGCGGG TGGGGCCAGA GAAAAGGCCC 
AAATCTTCGA GGGATGGTCC AGGAGGACCC CAGGAGGCCT CCCGCGATAA GCGCCCACTC 
TCTGGGCCTG ATGTCAGCAC TCCTCAGCCT GGCAGTCTGA CCAGCGGGAC AAAACTAGCA 
GCTGGCAGGC CCTTTAACAC ATACCCACGG GCTGACACGG ACCACCCACC CCGCGGTGCC 
CAGGGGGAGC CACATACCAT GGCCCCTAAT GGGCCTTCAG CCACAGGCCT GGCCGCTCCT 
CAGTCTTCCT CCTCCTCCCG GCCTCCCACC CGAGCCCGTG GTGCTCCCAG CCCAGGAGTT 
CTGGGTCCCC ATGCCTCTGA GCCCCAGTTG GCCCCCCCAG CACGTGCCCT TGCTGCCCCT 
GCTGTACCTC CTGCCCCTGG GCCCCCTGGG CCTCGCTCAC CACAGCGGGA GCCCCAACGA 
GTGTCCCATG AGCAGTTCCG GGCTGCCCTG CAGCTGGTGG TGGACCCAGG GGACCCTCGT 
TCCTATCTAG ACAACTTCAT CAAGATTGGT GAGGGCTCTA CAGGTATTGT GTGCATTGCC 
ACTGTACGCA GCTCAGGCAA ACTGGTGGCC GTCAAGAAGA TGGACTTGCG CAAGCAGCAA 
AGACGTGAAC TGCTCTTCAA TGAGGTGGTG ATCATGCGGG ACTACCGGCA CGAGAACGTG 
GTGGAGATGT ACAACAGCTA CCTGGTGGGT GACGAACTCT GGGTCGTCAT GGAGTTCCTG 
GAAGGCGGCG CCCTCACGGA TATTGTCACC CACACCAGGA TGAACGAGGA ACAGATCGCC 
GCCGTGTGCC TGGCTGTGCT TCAGGCGCTG GCTGTGCTCC ACGCCCAGGG TGTCATCCAC 
CGTGACATCA AGAGTGACTC TATCTTGCTG ACCCATGATG GCCGGGTGAA GCTGTCCGAC 
TTCGGGTTTT GTGCCCAGGT GAGCAAGGAG GTGCCTCGGA GGAAGTCGCT GGTGGGCACC 
CCGTATTGGA TGGCCCCGGA GCTCATCTCC CGCCTTCCCT ATGGGCCAGA GGTGGATATC 
TGGTCACTGG GGGTGATGGT GATCGAGATG GTGGATGGGG AGCCCCCTTA CTTCAACGAG 
CCACCCCTCA AAGCTATGAA GATGATCCGG GACAACCTCC CGCCCCGACT GAAGAACCTG 
CACAAGGCGT CACCGTCTCT GAAAGGCTTC TTGGATCGCC TGCTAGTGCG GGACCCGGCC 
CAGCGGGCCA CTGCTGCCGA GCTGCTGAAG CACCCGTTCC TCACCAAGGC GGGGCCACCA 
GCCAGCATCG TGCCCCTGAT GCGCCAGCAC CGAACCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081746.1
CDS	286..2067
Translation	MFGKKKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLIDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRESPPPPARAHQENGMLEERAAPARMAPDKAGSRARATGHSEAGSGSGDRRRVGPEKRPKSSRDGPGGPQEASRDKRPLSGPDVSTPQPGSLTSGTKLAAGRPFNTYPRADTDHPPRGAQGEPHTMAPNGPSATGLAAPQSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPARALAAPAVPPAPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYRHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALAVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGVMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKASPSLKGFLDRLLVRDPAQRATAAELLKHPFLTKAGPPASIVPLMRQHRTR

Target ORF information:

RefSeq Version	NM_027470.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus p21 (RAC1) activated kinase 4 (Pak4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027470.3

ORF Insert Sequence:

ATGTTTGGAA AGAAGAAGAA GCGGGTGGAG ATCTCAGCCC CATCCAACTT CGAGCACCGT 
GTACACACAG GCTTTGATCA GCATGAGCAG AAGTTCACAG GGCTGCCCCG CCAGTGGCAG 
AGCCTGATTG AGGAATCAGC ACGCAGACCC AAGCCCCTGA TCGACCCTGC CTGCATCACG 
TCCATCCAGC CTGGAGCCCC CAAGACCATC GTTCGGGGCA GCAAAGGTGC CAAGGATGGG 
GCTCTCACTC TGCTGCTTGA CGAATTTGAG AACATGTCGG TGACGCGCTC CAACTCCCTA 
CGCAGAGAGA GTCCACCACC ACCTGCCCGT GCCCACCAGG AGAATGGAAT GCTGGAGGAG 
CGGGCAGCCC CGGCCAGAAT GGCCCCTGAC AAAGCTGGAA GCAGAGCCCG GGCCACAGGC 
CACAGTGAGG CAGGCAGTGG CAGTGGTGAC AGACGGCGGG TGGGGCCAGA GAAAAGGCCC 
AAATCTTCGA GGGATGGTCC AGGAGGACCC CAGGAGGCCT CCCGCGATAA GCGCCCACTC 
TCTGGGCCTG ATGTCAGCAC TCCTCAGCCT GGCAGTCTGA CCAGCGGGAC AAAACTAGCA 
GCTGGCAGGC CCTTTAACAC ATACCCACGG GCTGACACGG ACCACCCACC CCGCGGTGCC 
CAGGGGGAGC CACATACCAT GGCCCCTAAT GGGCCTTCAG CCACAGGCCT GGCCGCTCCT 
CAGTCTTCCT CCTCCTCCCG GCCTCCCACC CGAGCCCGTG GTGCTCCCAG CCCAGGAGTT 
CTGGGTCCCC ATGCCTCTGA GCCCCAGTTG GCCCCCCCAG CACGTGCCCT TGCTGCCCCT 
GCTGTACCTC CTGCCCCTGG GCCCCCTGGG CCTCGCTCAC CACAGCGGGA GCCCCAACGA 
GTGTCCCATG AGCAGTTCCG GGCTGCCCTG CAGCTGGTGG TGGACCCAGG GGACCCTCGT 
TCCTATCTAG ACAACTTCAT CAAGATTGGT GAGGGCTCTA CAGGTATTGT GTGCATTGCC 
ACTGTACGCA GCTCAGGCAA ACTGGTGGCC GTCAAGAAGA TGGACTTGCG CAAGCAGCAA 
AGACGTGAAC TGCTCTTCAA TGAGGTGGTG ATCATGCGGG ACTACCGGCA CGAGAACGTG 
GTGGAGATGT ACAACAGCTA CCTGGTGGGT GACGAACTCT GGGTCGTCAT GGAGTTCCTG 
GAAGGCGGCG CCCTCACGGA TATTGTCACC CACACCAGGA TGAACGAGGA ACAGATCGCC 
GCCGTGTGCC TGGCTGTGCT TCAGGCGCTG GCTGTGCTCC ACGCCCAGGG TGTCATCCAC 
CGTGACATCA AGAGTGACTC TATCTTGCTG ACCCATGATG GCCGGGTGAA GCTGTCCGAC 
TTCGGGTTTT GTGCCCAGGT GAGCAAGGAG GTGCCTCGGA GGAAGTCGCT GGTGGGCACC 
CCGTATTGGA TGGCCCCGGA GCTCATCTCC CGCCTTCCCT ATGGGCCAGA GGTGGATATC 
TGGTCACTGG GGGTGATGGT GATCGAGATG GTGGATGGGG AGCCCCCTTA CTTCAACGAG 
CCACCCCTCA AAGCTATGAA GATGATCCGG GACAACCTCC CGCCCCGACT GAAGAACCTG 
CACAAGGCGT CACCGTCTCT GAAAGGCTTC TTGGATCGCC TGCTAGTGCG GGACCCGGCC 
CAGCGGGCCA CTGCTGCCGA GCTGCTGAAG CACCCGTTCC TCACCAAGGC GGGGCCACCA 
GCCAGCATCG TGCCCCTGAT GCGCCAGCAC CGAACCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pak4 ( XM_017312296.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167785.1 Mus musculus p21 (RAC1) activated kinase 4 (Pak4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu14793
Clone ID Related Accession (Same CDS sequence)	XM_017312296.1 , NM_027470.3 , XM_017312297.1
Accession Version	XM_017312296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase PAK 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAA AGAAGAAGAA GCGGGTGGAG ATCTCAGCCC CATCCAACTT CGAGCACCGT 
GTACACACAG GCTTTGATCA GCATGAGCAG AAGTTCACAG GGCTGCCCCG CCAGTGGCAG 
AGCCTGATTG AGGAATCAGC ACGCAGACCC AAGCCCCTGA TCGACCCTGC CTGCATCACG 
TCCATCCAGC CTGGAGCCCC CAAGACCATC GTTCGGGGCA GCAAAGGTGC CAAGGATGGG 
GCTCTCACTC TGCTGCTTGA CGAATTTGAG AACATGTCGG TGACGCGCTC CAACTCCCTA 
CGCAGAGAGA GTCCACCACC ACCTGCCCGT GCCCACCAGG AGAATGGAAT GCTGGAGGAG 
CGGGCAGCCC CGGCCAGAAT GGCCCCTGAC AAAGCTGGAA GCAGAGCCCG GGCCACAGGC 
CACAGTGAGG CAGGCAGTGG CAGTGGTGAC AGACGGCGGG TGGGGCCAGA GAAAAGGCCC 
AAATCTTCGA GGGATGGTCC AGGAGGACCC CAGGAGGCCT CCCGCGATAA GCGCCCACTC 
TCTGGGCCTG ATGTCAGCAC TCCTCAGCCT GGCAGTCTGA CCAGCGGGAC AAAACTAGCA 
GCTGGCAGGC CCTTTAACAC ATACCCACGG GCTGACACGG ACCACCCACC CCGCGGTGCC 
CAGGGGGAGC CACATACCAT GGCCCCTAAT GGGCCTTCAG CCACAGGCCT GGCCGCTCCT 
CAGTCTTCCT CCTCCTCCCG GCCTCCCACC CGAGCCCGTG GTGCTCCCAG CCCAGGAGTT 
CTGGGTCCCC ATGCCTCTGA GCCCCAGTTG GCCCCCCCAG CACGTGCCCT TGCTGCCCCT 
GCTGTACCTC CTGCCCCTGG GCCCCCTGGG CCTCGCTCAC CACAGCGGGA GCCCCAACGA 
GTGTCCCATG AGCAGTTCCG GGCTGCCCTG CAGCTGGTGG TGGACCCAGG GGACCCTCGT 
TCCTATCTAG ACAACTTCAT CAAGATTGGT GAGGGCTCTA CAGGTATTGT GTGCATTGCC 
ACTGTACGCA GCTCAGGCAA ACTGGTGGCC GTCAAGAAGA TGGACTTGCG CAAGCAGCAA 
AGACGTGAAC TGCTCTTCAA TGAGGTGGTG ATCATGCGGG ACTACCGGCA CGAGAACGTG 
GTGGAGATGT ACAACAGCTA CCTGGTGGGT GACGAACTCT GGGTCGTCAT GGAGTTCCTG 
GAAGGCGGCG CCCTCACGGA TATTGTCACC CACACCAGGA TGAACGAGGA ACAGATCGCC 
GCCGTGTGCC TGGCTGTGCT TCAGGCGCTG GCTGTGCTCC ACGCCCAGGG TGTCATCCAC 
CGTGACATCA AGAGTGACTC TATCTTGCTG ACCCATGATG GCCGGGTGAA GCTGTCCGAC 
TTCGGGTTTT GTGCCCAGGT GAGCAAGGAG GTGCCTCGGA GGAAGTCGCT GGTGGGCACC 
CCGTATTGGA TGGCCCCGGA GCTCATCTCC CGCCTTCCCT ATGGGCCAGA GGTGGATATC 
TGGTCACTGG GGGTGATGGT GATCGAGATG GTGGATGGGG AGCCCCCTTA CTTCAACGAG 
CCACCCCTCA AAGCTATGAA GATGATCCGG GACAACCTCC CGCCCCGACT GAAGAACCTG 
CACAAGGCGT CACCGTCTCT GAAAGGCTTC TTGGATCGCC TGCTAGTGCG GGACCCGGCC 
CAGCGGGCCA CTGCTGCCGA GCTGCTGAAG CACCCGTTCC TCACCAAGGC GGGGCCACCA 
GCCAGCATCG TGCCCCTGAT GCGCCAGCAC CGAACCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167785.1
CDS	428..2209
Translation	MFGKKKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLIDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRESPPPPARAHQENGMLEERAAPARMAPDKAGSRARATGHSEAGSGSGDRRRVGPEKRPKSSRDGPGGPQEASRDKRPLSGPDVSTPQPGSLTSGTKLAAGRPFNTYPRADTDHPPRGAQGEPHTMAPNGPSATGLAAPQSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPARALAAPAVPPAPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYRHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALAVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGVMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKASPSLKGFLDRLLVRDPAQRATAAELLKHPFLTKAGPPASIVPLMRQHRTR

Target ORF information:

RefSeq Version	XM_017312296.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus p21 (RAC1) activated kinase 4 (Pak4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312296.1

ORF Insert Sequence:

ATGTTTGGAA AGAAGAAGAA GCGGGTGGAG ATCTCAGCCC CATCCAACTT CGAGCACCGT 
GTACACACAG GCTTTGATCA GCATGAGCAG AAGTTCACAG GGCTGCCCCG CCAGTGGCAG 
AGCCTGATTG AGGAATCAGC ACGCAGACCC AAGCCCCTGA TCGACCCTGC CTGCATCACG 
TCCATCCAGC CTGGAGCCCC CAAGACCATC GTTCGGGGCA GCAAAGGTGC CAAGGATGGG 
GCTCTCACTC TGCTGCTTGA CGAATTTGAG AACATGTCGG TGACGCGCTC CAACTCCCTA 
CGCAGAGAGA GTCCACCACC ACCTGCCCGT GCCCACCAGG AGAATGGAAT GCTGGAGGAG 
CGGGCAGCCC CGGCCAGAAT GGCCCCTGAC AAAGCTGGAA GCAGAGCCCG GGCCACAGGC 
CACAGTGAGG CAGGCAGTGG CAGTGGTGAC AGACGGCGGG TGGGGCCAGA GAAAAGGCCC 
AAATCTTCGA GGGATGGTCC AGGAGGACCC CAGGAGGCCT CCCGCGATAA GCGCCCACTC 
TCTGGGCCTG ATGTCAGCAC TCCTCAGCCT GGCAGTCTGA CCAGCGGGAC AAAACTAGCA 
GCTGGCAGGC CCTTTAACAC ATACCCACGG GCTGACACGG ACCACCCACC CCGCGGTGCC 
CAGGGGGAGC CACATACCAT GGCCCCTAAT GGGCCTTCAG CCACAGGCCT GGCCGCTCCT 
CAGTCTTCCT CCTCCTCCCG GCCTCCCACC CGAGCCCGTG GTGCTCCCAG CCCAGGAGTT 
CTGGGTCCCC ATGCCTCTGA GCCCCAGTTG GCCCCCCCAG CACGTGCCCT TGCTGCCCCT 
GCTGTACCTC CTGCCCCTGG GCCCCCTGGG CCTCGCTCAC CACAGCGGGA GCCCCAACGA 
GTGTCCCATG AGCAGTTCCG GGCTGCCCTG CAGCTGGTGG TGGACCCAGG GGACCCTCGT 
TCCTATCTAG ACAACTTCAT CAAGATTGGT GAGGGCTCTA CAGGTATTGT GTGCATTGCC 
ACTGTACGCA GCTCAGGCAA ACTGGTGGCC GTCAAGAAGA TGGACTTGCG CAAGCAGCAA 
AGACGTGAAC TGCTCTTCAA TGAGGTGGTG ATCATGCGGG ACTACCGGCA CGAGAACGTG 
GTGGAGATGT ACAACAGCTA CCTGGTGGGT GACGAACTCT GGGTCGTCAT GGAGTTCCTG 
GAAGGCGGCG CCCTCACGGA TATTGTCACC CACACCAGGA TGAACGAGGA ACAGATCGCC 
GCCGTGTGCC TGGCTGTGCT TCAGGCGCTG GCTGTGCTCC ACGCCCAGGG TGTCATCCAC 
CGTGACATCA AGAGTGACTC TATCTTGCTG ACCCATGATG GCCGGGTGAA GCTGTCCGAC 
TTCGGGTTTT GTGCCCAGGT GAGCAAGGAG GTGCCTCGGA GGAAGTCGCT GGTGGGCACC 
CCGTATTGGA TGGCCCCGGA GCTCATCTCC CGCCTTCCCT ATGGGCCAGA GGTGGATATC 
TGGTCACTGG GGGTGATGGT GATCGAGATG GTGGATGGGG AGCCCCCTTA CTTCAACGAG 
CCACCCCTCA AAGCTATGAA GATGATCCGG GACAACCTCC CGCCCCGACT GAAGAACCTG 
CACAAGGCGT CACCGTCTCT GAAAGGCTTC TTGGATCGCC TGCTAGTGCG GGACCCGGCC 
CAGCGGGCCA CTGCTGCCGA GCTGCTGAAG CACCCGTTCC TCACCAAGGC GGGGCCACCA 
GCCAGCATCG TGCCCCTGAT GCGCCAGCAC CGAACCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pak4 ( XM_017312297.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167786.1 Mus musculus p21 (RAC1) activated kinase 4 (Pak4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu14793
Clone ID Related Accession (Same CDS sequence)	XM_017312296.1 , NM_027470.3 , XM_017312297.1
Accession Version	XM_017312297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase PAK 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAA AGAAGAAGAA GCGGGTGGAG ATCTCAGCCC CATCCAACTT CGAGCACCGT 
GTACACACAG GCTTTGATCA GCATGAGCAG AAGTTCACAG GGCTGCCCCG CCAGTGGCAG 
AGCCTGATTG AGGAATCAGC ACGCAGACCC AAGCCCCTGA TCGACCCTGC CTGCATCACG 
TCCATCCAGC CTGGAGCCCC CAAGACCATC GTTCGGGGCA GCAAAGGTGC CAAGGATGGG 
GCTCTCACTC TGCTGCTTGA CGAATTTGAG AACATGTCGG TGACGCGCTC CAACTCCCTA 
CGCAGAGAGA GTCCACCACC ACCTGCCCGT GCCCACCAGG AGAATGGAAT GCTGGAGGAG 
CGGGCAGCCC CGGCCAGAAT GGCCCCTGAC AAAGCTGGAA GCAGAGCCCG GGCCACAGGC 
CACAGTGAGG CAGGCAGTGG CAGTGGTGAC AGACGGCGGG TGGGGCCAGA GAAAAGGCCC 
AAATCTTCGA GGGATGGTCC AGGAGGACCC CAGGAGGCCT CCCGCGATAA GCGCCCACTC 
TCTGGGCCTG ATGTCAGCAC TCCTCAGCCT GGCAGTCTGA CCAGCGGGAC AAAACTAGCA 
GCTGGCAGGC CCTTTAACAC ATACCCACGG GCTGACACGG ACCACCCACC CCGCGGTGCC 
CAGGGGGAGC CACATACCAT GGCCCCTAAT GGGCCTTCAG CCACAGGCCT GGCCGCTCCT 
CAGTCTTCCT CCTCCTCCCG GCCTCCCACC CGAGCCCGTG GTGCTCCCAG CCCAGGAGTT 
CTGGGTCCCC ATGCCTCTGA GCCCCAGTTG GCCCCCCCAG CACGTGCCCT TGCTGCCCCT 
GCTGTACCTC CTGCCCCTGG GCCCCCTGGG CCTCGCTCAC CACAGCGGGA GCCCCAACGA 
GTGTCCCATG AGCAGTTCCG GGCTGCCCTG CAGCTGGTGG TGGACCCAGG GGACCCTCGT 
TCCTATCTAG ACAACTTCAT CAAGATTGGT GAGGGCTCTA CAGGTATTGT GTGCATTGCC 
ACTGTACGCA GCTCAGGCAA ACTGGTGGCC GTCAAGAAGA TGGACTTGCG CAAGCAGCAA 
AGACGTGAAC TGCTCTTCAA TGAGGTGGTG ATCATGCGGG ACTACCGGCA CGAGAACGTG 
GTGGAGATGT ACAACAGCTA CCTGGTGGGT GACGAACTCT GGGTCGTCAT GGAGTTCCTG 
GAAGGCGGCG CCCTCACGGA TATTGTCACC CACACCAGGA TGAACGAGGA ACAGATCGCC 
GCCGTGTGCC TGGCTGTGCT TCAGGCGCTG GCTGTGCTCC ACGCCCAGGG TGTCATCCAC 
CGTGACATCA AGAGTGACTC TATCTTGCTG ACCCATGATG GCCGGGTGAA GCTGTCCGAC 
TTCGGGTTTT GTGCCCAGGT GAGCAAGGAG GTGCCTCGGA GGAAGTCGCT GGTGGGCACC 
CCGTATTGGA TGGCCCCGGA GCTCATCTCC CGCCTTCCCT ATGGGCCAGA GGTGGATATC 
TGGTCACTGG GGGTGATGGT GATCGAGATG GTGGATGGGG AGCCCCCTTA CTTCAACGAG 
CCACCCCTCA AAGCTATGAA GATGATCCGG GACAACCTCC CGCCCCGACT GAAGAACCTG 
CACAAGGCGT CACCGTCTCT GAAAGGCTTC TTGGATCGCC TGCTAGTGCG GGACCCGGCC 
CAGCGGGCCA CTGCTGCCGA GCTGCTGAAG CACCCGTTCC TCACCAAGGC GGGGCCACCA 
GCCAGCATCG TGCCCCTGAT GCGCCAGCAC CGAACCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167786.1
CDS	282..2063
Translation	MFGKKKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLIDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRESPPPPARAHQENGMLEERAAPARMAPDKAGSRARATGHSEAGSGSGDRRRVGPEKRPKSSRDGPGGPQEASRDKRPLSGPDVSTPQPGSLTSGTKLAAGRPFNTYPRADTDHPPRGAQGEPHTMAPNGPSATGLAAPQSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPARALAAPAVPPAPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYRHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALAVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGVMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKASPSLKGFLDRLLVRDPAQRATAAELLKHPFLTKAGPPASIVPLMRQHRTR

Target ORF information:

RefSeq Version	XM_017312297.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus p21 (RAC1) activated kinase 4 (Pak4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312297.1

ORF Insert Sequence:

ATGTTTGGAA AGAAGAAGAA GCGGGTGGAG ATCTCAGCCC CATCCAACTT CGAGCACCGT 
GTACACACAG GCTTTGATCA GCATGAGCAG AAGTTCACAG GGCTGCCCCG CCAGTGGCAG 
AGCCTGATTG AGGAATCAGC ACGCAGACCC AAGCCCCTGA TCGACCCTGC CTGCATCACG 
TCCATCCAGC CTGGAGCCCC CAAGACCATC GTTCGGGGCA GCAAAGGTGC CAAGGATGGG 
GCTCTCACTC TGCTGCTTGA CGAATTTGAG AACATGTCGG TGACGCGCTC CAACTCCCTA 
CGCAGAGAGA GTCCACCACC ACCTGCCCGT GCCCACCAGG AGAATGGAAT GCTGGAGGAG 
CGGGCAGCCC CGGCCAGAAT GGCCCCTGAC AAAGCTGGAA GCAGAGCCCG GGCCACAGGC 
CACAGTGAGG CAGGCAGTGG CAGTGGTGAC AGACGGCGGG TGGGGCCAGA GAAAAGGCCC 
AAATCTTCGA GGGATGGTCC AGGAGGACCC CAGGAGGCCT CCCGCGATAA GCGCCCACTC 
TCTGGGCCTG ATGTCAGCAC TCCTCAGCCT GGCAGTCTGA CCAGCGGGAC AAAACTAGCA 
GCTGGCAGGC CCTTTAACAC ATACCCACGG GCTGACACGG ACCACCCACC CCGCGGTGCC 
CAGGGGGAGC CACATACCAT GGCCCCTAAT GGGCCTTCAG CCACAGGCCT GGCCGCTCCT 
CAGTCTTCCT CCTCCTCCCG GCCTCCCACC CGAGCCCGTG GTGCTCCCAG CCCAGGAGTT 
CTGGGTCCCC ATGCCTCTGA GCCCCAGTTG GCCCCCCCAG CACGTGCCCT TGCTGCCCCT 
GCTGTACCTC CTGCCCCTGG GCCCCCTGGG CCTCGCTCAC CACAGCGGGA GCCCCAACGA 
GTGTCCCATG AGCAGTTCCG GGCTGCCCTG CAGCTGGTGG TGGACCCAGG GGACCCTCGT 
TCCTATCTAG ACAACTTCAT CAAGATTGGT GAGGGCTCTA CAGGTATTGT GTGCATTGCC 
ACTGTACGCA GCTCAGGCAA ACTGGTGGCC GTCAAGAAGA TGGACTTGCG CAAGCAGCAA 
AGACGTGAAC TGCTCTTCAA TGAGGTGGTG ATCATGCGGG ACTACCGGCA CGAGAACGTG 
GTGGAGATGT ACAACAGCTA CCTGGTGGGT GACGAACTCT GGGTCGTCAT GGAGTTCCTG 
GAAGGCGGCG CCCTCACGGA TATTGTCACC CACACCAGGA TGAACGAGGA ACAGATCGCC 
GCCGTGTGCC TGGCTGTGCT TCAGGCGCTG GCTGTGCTCC ACGCCCAGGG TGTCATCCAC 
CGTGACATCA AGAGTGACTC TATCTTGCTG ACCCATGATG GCCGGGTGAA GCTGTCCGAC 
TTCGGGTTTT GTGCCCAGGT GAGCAAGGAG GTGCCTCGGA GGAAGTCGCT GGTGGGCACC 
CCGTATTGGA TGGCCCCGGA GCTCATCTCC CGCCTTCCCT ATGGGCCAGA GGTGGATATC 
TGGTCACTGG GGGTGATGGT GATCGAGATG GTGGATGGGG AGCCCCCTTA CTTCAACGAG 
CCACCCCTCA AAGCTATGAA GATGATCCGG GACAACCTCC CGCCCCGACT GAAGAACCTG 
CACAAGGCGT CACCGTCTCT GAAAGGCTTC TTGGATCGCC TGCTAGTGCG GGACCCGGCC 
CAGCGGGCCA CTGCTGCCGA GCTGCTGAAG CACCCGTTCC TCACCAAGGC GGGGCCACCA 
GCCAGCATCG TGCCCCTGAT GCGCCAGCAC CGAACCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAK4 crystal structures suggest unusual kinase conformational movements.
Biochimica et biophysica acta1866(2)356-365(2018 Feb)
Zhang EY,Ha BH,Boggon TJ

Pdx1-Cre-driven conditional gene depletion suggests PAK4 as dispensable for mouse pancreas development.
Scientific reports7(1)7031(2017 Aug)
Zhao M,Rabieifar P,Costa TDF,Zhuang T,Minden A,L?hr M,Heuchel R,Str?mblad S

Increased Circulating Endothelial Microparticles Associated with PAK4 Play a Key Role in Ventilation-Induced Lung Injury Process.
BioMed research international20174902084(2017)
Pan S,Fei A,Jing L,Zhang X,Gao C

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Bone research214034(2014)
Brommage R,Liu J,Hansen GM,Kirkpatrick LL,Potter DG,Sands AT,Zambrowicz B,Powell DR,Vogel P

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Pak4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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