Pspc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pspc1
Entrez Gene ID	66645
Full Name	paraspeckle protein 1
Synonyms	5730470C09Rik,AI327109,AI449052,PSP1
General protein information	Preferred Names paraspeckle protein 1 Names paraspeckle component 1 mPSP1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_025682.4	NP_079958.3	paraspeckle component 1 isoform 1
XM_006519393.2	XP_006519456.1	paraspeckle component 1 isoform X1
NM_001360420.1	NP_001347349.1	paraspeckle component 1 isoform 2
NM_025682.3	NP_079958.3	paraspeckle component 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSPC1	NP_001035879.1
Bos taurus (cattle)	PSPC1	NP_001068745.1
Pan troglodytes (chimpanzee)	PSPC1	XP_001144461.1
	PSPC1	XP_001088371.1
Danio rerio (zebrafish)	pspc1	NP_001038723.1
Xenopus tropicalis (tropical clawed frog)	pspc1	XP_002938004.2
Rattus norvegicus (Norway rat)	Pspc1	NP_001020843.1
Canis lupus familiaris (dog)	PSPC1	XP_003639923.1
Mus musculus (house mouse)	Pspc1	NP_079958.3
Gallus gallus (chicken)	PSPC1	NP_001026002.1

Gene Ontology
Bibliography

Related articles in PubMed

RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.
Wang J, Rajbhandari P, Damianov A, Han A, Sallam T, Waki H, Villanueva CJ, Lee SD, Nielsen R, Mandrup S, Reue K, Young SG, Whitelegge J, Saez E, Black DL, Tontonoz P
The Journal of clinical investigation127(3)987-1004(2017 Mar)

Synergistic upregulation of NONO and PSPC1 regulates Sertoli cell response to MEHP via modulation of ALDH1A1 signaling.
Dong BW, Jin XH, Yan CY, Yang T, Cai GQ, Lu J
FEBS letters591(6)914-923(2017 Mar)

Specific interaction with the nuclear transporter importin ?2 can modulate paraspeckle protein 1 delivery to nuclear paraspeckles.
Major AT, Hogarth CA, Miyamoto Y, Sarraj MA, Smith CL, Koopman P, Kurihara Y, Jans DA, Loveland KL
Molecular biology of the cell26(8)1543-58(2015 Apr)

Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog.
Li S, Li Z, Shu FJ, Xiong H, Phillips AC, Dynan WS
Nucleic acids research42(15)9771-80(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings highlight a role for PSPC1-dependent RNA maturation in the posttranscriptional control of adipose development and function.
Title: RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.

Data indicate involvement of non-POU domain-containing octamer-binding protein (NONO)/paraspeckle protein component 1 (PSPC1)-aldehyde dehydrogenase 1 (NONO/PSPC1-ALDH1A1) in the modulation of Sertoli cells (SCs) response to mono-(2-ethylhexyl) phthalate (MEHP) challenge.
Title: Synergistic upregulation of NONO and PSPC1 regulates Sertoli cell response to MEHP via modulation of ALDH1A1 signaling.

Specific interaction with the nuclear transporter importin alpha2 can modulate paraspeckle protein 1 delivery to nuclear paraspeckles.
Title: Specific interaction with the nuclear transporter importin ?2 can modulate paraspeckle protein 1 delivery to nuclear paraspeckles.

NONO genetic insufficiency led to upregulation of PSPC1, which replaced NONO in a stable complex with SFPQ.
Title: Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog.

A protein complex was identified containing PSPC1, PSF and LMX1B, suggesting the existence of this complex in mesodiencephalic dopaminergic neurons.
Title: LMX1B is part of a transcriptional complex with PSPC1 and PSF.

The following Pspc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pspc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50247	NM_001360420.1 Latest version!	Mus musculus paraspeckle protein 1 (Pspc1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu50247	XM_006519393.2 Latest version!	Mus musculus paraspeckle protein 1 (Pspc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu00865	NM_025682.4 Latest version!	Mus musculus paraspeckle protein 1 (Pspc1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OMu00865	NM_025682.3	Mus musculus paraspeckle protein 1 (Pspc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pspc1 ( NM_001360420.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347349.1 Mus musculus paraspeckle protein 1 (Pspc1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu50247
Clone ID Related Accession (Same CDS sequence)	XM_006519393.2 , NM_001360420.1
Accession Version	NM_001360420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	paraspeckle component 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB244768.1, CN528008.1, AK011272.1, AK017689.1 and AC154731.2. On Feb 3, 2018 this sequence version replaced XM_006519393.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.282238.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGT TTGATGATGA AGATGGTTTA CCAGAGAAGC TAATGCAGAA AACTCAACAG 
TACCATAAGG AAAGAGAACA GCCACCACGG TTTGCTCAAC CTGGGACATT TGAATTTGAA 
TATGCGTCTC GATGGAAGGC TCTTGATGAA ATGGAAAAGC AGCAGCGGGA ACAAGTTGAT 
AGAAACATCA GAGAAGCCAA AGAGAAACTG GAGGCAGAGA TGGAAGCAGC TAGACATGAA 
CACCAGTTAA TGCTAATGAG GCAAGATCTA ATGAGACGTC AAGAAGAACT CAGACGTTTG 
GAAGAACTCA GAAATCAAGA ACTGCAGAAA CGAAAGCAAA TACAACTGAG ACACGAAGAA 
GAGCATCGTC GTCGTGAAGA AGAAATGATC CGTCACAGAG AGCAGGAGGA GCTGAGGCGA 
CAGCAAGAGG GCGGCTTTAA ACCAAACTAT ATGGAAAATA GAGAACAGGA AATGAGAATG 
GGTGATATGG GTCCCCGTGG AGCAATAAAC ATGGGAGATG CATTTAGCCC AGCACCTGCT 
GGTACCCAAG GTCCTCCTCC AATGATGGGT ATGAATATGA ACAACAGAGG AACTATACCT 
GGCCCACCAA TGGGTCCTGG TCCTGCCATG GGACCAGAAG GAGCTGCAAA TATGGGAACT 
CCAATGATAC CAGATAATGG CGCAGTGCAC AATGATAGGT TTCCCCAAGG ACCTCCATCC 
CAGATGGGTT CACCCATGGG AAATAGAACA GGTTCTGAAA CTCCCCAGGC ACCGATGAGT 
GGTGTAGGGC CTGTAAGTGG TGGTCCTGGC GGCTTTGGTA GAGGGAGTCA AGGGGGCAAT 
TTTGAAGGCC CTAATAAGCG TCGGAGATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347349.1
CDS	557..1429
Translation	MEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGTQGPPPMMGMNMNNRGTIPGPPMGPGPAMGPEGAANMGTPMIPDNGAVHNDRFPQGPPSQMGSPMGNRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY

Target ORF information:

RefSeq Version	NM_001360420.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus paraspeckle protein 1 (Pspc1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360420.1

ORF Insert Sequence:

ATGGAGCAGT TTGATGATGA AGATGGTTTA CCAGAGAAGC TAATGCAGAA AACTCAACAG 
TACCATAAGG AAAGAGAACA GCCACCACGG TTTGCTCAAC CTGGGACATT TGAATTTGAA 
TATGCGTCTC GATGGAAGGC TCTTGATGAA ATGGAAAAGC AGCAGCGGGA ACAAGTTGAT 
AGAAACATCA GAGAAGCCAA AGAGAAACTG GAGGCAGAGA TGGAAGCAGC TAGACATGAA 
CACCAGTTAA TGCTAATGAG GCAAGATCTA ATGAGACGTC AAGAAGAACT CAGACGTTTG 
GAAGAACTCA GAAATCAAGA ACTGCAGAAA CGAAAGCAAA TACAACTGAG ACACGAAGAA 
GAGCATCGTC GTCGTGAAGA AGAAATGATC CGTCACAGAG AGCAGGAGGA GCTGAGGCGA 
CAGCAAGAGG GCGGCTTTAA ACCAAACTAT ATGGAAAATA GAGAACAGGA AATGAGAATG 
GGTGATATGG GTCCCCGTGG AGCAATAAAC ATGGGAGATG CATTTAGCCC AGCACCTGCT 
GGTACCCAAG GTCCTCCTCC AATGATGGGT ATGAATATGA ACAACAGAGG AACTATACCT 
GGCCCACCAA TGGGTCCTGG TCCTGCCATG GGACCAGAAG GAGCTGCAAA TATGGGAACT 
CCAATGATAC CAGATAATGG CGCAGTGCAC AATGATAGGT TTCCCCAAGG ACCTCCATCC 
CAGATGGGTT CACCCATGGG AAATAGAACA GGTTCTGAAA CTCCCCAGGC ACCGATGAGT 
GGTGTAGGGC CTGTAAGTGG TGGTCCTGGC GGCTTTGGTA GAGGGAGTCA AGGGGGCAAT 
TTTGAAGGCC CTAATAAGCG TCGGAGATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pspc1 ( XM_006519393.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519456.1 Mus musculus paraspeckle protein 1 (Pspc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50247
Clone ID Related Accession (Same CDS sequence)	XM_006519393.2 , NM_001360420.1
Accession Version	XM_006519393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	paraspeckle component 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TTGATGATGA AGATGGTTTA CCAGAGAAGC TAATGCAGAA AACTCAACAG 
TACCATAAGG AAAGAGAACA GCCACCACGG TTTGCTCAAC CTGGGACATT TGAATTTGAA 
TATGCGTCTC GATGGAAGGC TCTTGATGAA ATGGAAAAGC AGCAGCGGGA ACAAGTTGAT 
AGAAACATCA GAGAAGCCAA AGAGAAACTG GAGGCAGAGA TGGAAGCAGC TAGACATGAA 
CACCAGTTAA TGCTAATGAG GCAAGATCTA ATGAGACGTC AAGAAGAACT CAGACGTTTG 
GAAGAACTCA GAAATCAAGA ACTGCAGAAA CGAAAGCAAA TACAACTGAG ACACGAAGAA 
GAGCATCGTC GTCGTGAAGA AGAAATGATC CGTCACAGAG AGCAGGAGGA GCTGAGGCGA 
CAGCAAGAGG GCGGCTTTAA ACCAAACTAT ATGGAAAATA GAGAACAGGA AATGAGAATG 
GGTGATATGG GTCCCCGTGG AGCAATAAAC ATGGGAGATG CATTTAGCCC AGCACCTGCT 
GGTACCCAAG GTCCTCCTCC AATGATGGGT ATGAATATGA ACAACAGAGG AACTATACCT 
GGCCCACCAA TGGGTCCTGG TCCTGCCATG GGACCAGAAG GAGCTGCAAA TATGGGAACT 
CCAATGATAC CAGATAATGG CGCAGTGCAC AATGATAGGT TTCCCCAAGG ACCTCCATCC 
CAGATGGGTT CACCCATGGG AAATAGAACA GGTTCTGAAA CTCCCCAGGC ACCGATGAGT 
GGTGTAGGGC CTGTAAGTGG TGGTCCTGGC GGCTTTGGTA GAGGGAGTCA AGGGGGCAAT 
TTTGAAGGCC CTAATAAGCG TCGGAGATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519456.1
CDS	235..1107
Translation	MEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGTQGPPPMMGMNMNNRGTIPGPPMGPGPAMGPEGAANMGTPMIPDNGAVHNDRFPQGPPSQMGSPMGNRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY

Target ORF information:

RefSeq Version	XM_006519393.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus paraspeckle protein 1 (Pspc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519393.2

ORF Insert Sequence:

ATGGAGCAGT TTGATGATGA AGATGGTTTA CCAGAGAAGC TAATGCAGAA AACTCAACAG 
TACCATAAGG AAAGAGAACA GCCACCACGG TTTGCTCAAC CTGGGACATT TGAATTTGAA 
TATGCGTCTC GATGGAAGGC TCTTGATGAA ATGGAAAAGC AGCAGCGGGA ACAAGTTGAT 
AGAAACATCA GAGAAGCCAA AGAGAAACTG GAGGCAGAGA TGGAAGCAGC TAGACATGAA 
CACCAGTTAA TGCTAATGAG GCAAGATCTA ATGAGACGTC AAGAAGAACT CAGACGTTTG 
GAAGAACTCA GAAATCAAGA ACTGCAGAAA CGAAAGCAAA TACAACTGAG ACACGAAGAA 
GAGCATCGTC GTCGTGAAGA AGAAATGATC CGTCACAGAG AGCAGGAGGA GCTGAGGCGA 
CAGCAAGAGG GCGGCTTTAA ACCAAACTAT ATGGAAAATA GAGAACAGGA AATGAGAATG 
GGTGATATGG GTCCCCGTGG AGCAATAAAC ATGGGAGATG CATTTAGCCC AGCACCTGCT 
GGTACCCAAG GTCCTCCTCC AATGATGGGT ATGAATATGA ACAACAGAGG AACTATACCT 
GGCCCACCAA TGGGTCCTGG TCCTGCCATG GGACCAGAAG GAGCTGCAAA TATGGGAACT 
CCAATGATAC CAGATAATGG CGCAGTGCAC AATGATAGGT TTCCCCAAGG ACCTCCATCC 
CAGATGGGTT CACCCATGGG AAATAGAACA GGTTCTGAAA CTCCCCAGGC ACCGATGAGT 
GGTGTAGGGC CTGTAAGTGG TGGTCCTGGC GGCTTTGGTA GAGGGAGTCA AGGGGGCAAT 
TTTGAAGGCC CTAATAAGCG TCGGAGATAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pspc1 ( NM_025682.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079958.3 Mus musculus paraspeckle protein 1 (Pspc1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00865
Clone ID Related Accession (Same CDS sequence)	NM_025682.4 , NM_025682.3
Accession Version	NM_025682.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	paraspeckle component 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB244768.1, AK011272.1, AK017689.1 and AC154731.2. On Feb 3, 2018 this sequence version replaced NM_025682.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026772.1, AK017689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTTAA GAGGAAACCT GAAGCAAGTA CGTATTGAGA AAAACCCGGC TCGTCTTCGC 
GCCCTGGAGT CGGCGGCCGG AGAGAGCGAA CCGGTGGCCG CGGCGGCCAT GGCGCTGACG 
CTGGCCGGCG AGCAGGCGCC GCCACCCGCA CCTTCCGAGG AGCACCCGGA CGAGGAATTG 
GGCTTCACCA TCGACATCAA GAGTTTCCTC AAGCCGGGCG AGAAGACGTA CACGCAGCGC 
TGCCGCCTCT TCGTGGGTAA CCTGCCCACA GACATCACGG AGGAGGACTT CAAGCGCCTC 
TTTGAGCGCT ACGGAGAGCC CAGCGAGGTC TTCATCAACC GCGACCGAGG CTTCGGCTTC 
ATCCGCCTGG AATCGAGAAC ACTGGCTGAA ATTGCAAAAG CAGAGTTGGA TGGCACCATA 
CTGAAGAGCA GACCTCTCCG AATTCGCTTT GCTACACATG GAGCAGCCCT AACGGTCAAG 
AACCTTTCTC CGGTTGTTTC CAATGAGCTT CTAGAGCAAG CATTTTCCCA GTTTGGCCCA 
GTAGAGAAAG CTGTTGTGGT TGTGGATGAT CGTGGTAGAG CCACAGGAAA AGGTTTTGTA 
GAATTTGCAG CGAAACCTCC TGCACGAAAG GCTCTGGAGA GATGTGGTGA TGGGGCGTTC 
TTGCTAACAA CAACACCTCG TCCAGTCATT GTAGAACCCA TGGAGCAGTT TGATGATGAA 
GATGGTTTAC CAGAGAAGCT AATGCAGAAA ACTCAACAGT ACCATAAGGA AAGAGAACAG 
CCACCACGGT TTGCTCAACC TGGGACATTT GAATTTGAAT ATGCGTCTCG ATGGAAGGCT 
CTTGATGAAA TGGAAAAGCA GCAGCGGGAA CAAGTTGATA GAAACATCAG AGAAGCCAAA 
GAGAAACTGG AGGCAGAGAT GGAAGCAGCT AGACATGAAC ACCAGTTAAT GCTAATGAGG 
CAAGATCTAA TGAGACGTCA AGAAGAACTC AGACGTTTGG AAGAACTCAG AAATCAAGAA 
CTGCAGAAAC GAAAGCAAAT ACAACTGAGA CACGAAGAAG AGCATCGTCG TCGTGAAGAA 
GAAATGATCC GTCACAGAGA GCAGGAGGAG CTGAGGCGAC AGCAAGAGGG CGGCTTTAAA 
CCAAACTATA TGGAAAATAG AGAACAGGAA ATGAGAATGG GTGATATGGG TCCCCGTGGA 
GCAATAAACA TGGGAGATGC ATTTAGCCCA GCACCTGCTG GTACCCAAGG TCCTCCTCCA 
ATGATGGGTA TGAATATGAA CAACAGAGGA ACTATACCTG GCCCACCAAT GGGTCCTGGT 
CCTGCCATGG GACCAGAAGG AGCTGCAAAT ATGGGAACTC CAATGATACC AGATAATGGC 
GCAGTGCACA ATGATAGGTT TCCCCAAGGA CCTCCATCCC AGATGGGTTC ACCCATGGGA 
AATAGAACAG GTTCTGAAAC TCCCCAGGCA CCGATGAGTG GTGTAGGGCC TGTAAGTGGT 
GGTCCTGGCG GCTTTGGTAG AGGGAGTCAA GGGGGCAATT TTGAAGGCCC TAATAAGCGT 
CGGAGATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079958.3
CDS	160..1731
Translation	MMLRGNLKQVRIEKNPARLRALESAAGESEPVAAAAMALTLAGEQAPPPAPSEEHPDEELGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGTQGPPPMMGMNMNNRGTIPGPPMGPGPAMGPEGAANMGTPMIPDNGAVHNDRFPQGPPSQMGSPMGNRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY

Target ORF information:

RefSeq Version	NM_025682.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus paraspeckle protein 1 (Pspc1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025682.4

ORF Insert Sequence:

ATGATGTTAA GAGGAAACCT GAAGCAAGTA CGTATTGAGA AAAACCCGGC TCGTCTTCGC 
GCCCTGGAGT CGGCGGCCGG AGAGAGCGAA CCGGTGGCCG CGGCGGCCAT GGCGCTGACG 
CTGGCCGGCG AGCAGGCGCC GCCACCCGCA CCTTCCGAGG AGCACCCGGA CGAGGAATTG 
GGCTTCACCA TCGACATCAA GAGTTTCCTC AAGCCGGGCG AGAAGACGTA CACGCAGCGC 
TGCCGCCTCT TCGTGGGTAA CCTGCCCACA GACATCACGG AGGAGGACTT CAAGCGCCTC 
TTTGAGCGCT ACGGAGAGCC CAGCGAGGTC TTCATCAACC GCGACCGAGG CTTCGGCTTC 
ATCCGCCTGG AATCGAGAAC ACTGGCTGAA ATTGCAAAAG CAGAGTTGGA TGGCACCATA 
CTGAAGAGCA GACCTCTCCG AATTCGCTTT GCTACACATG GAGCAGCCCT AACGGTCAAG 
AACCTTTCTC CGGTTGTTTC CAATGAGCTT CTAGAGCAAG CATTTTCCCA GTTTGGCCCA 
GTAGAGAAAG CTGTTGTGGT TGTGGATGAT CGTGGTAGAG CCACAGGAAA AGGTTTTGTA 
GAATTTGCAG CGAAACCTCC TGCACGAAAG GCTCTGGAGA GATGTGGTGA TGGGGCGTTC 
TTGCTAACAA CAACACCTCG TCCAGTCATT GTAGAACCCA TGGAGCAGTT TGATGATGAA 
GATGGTTTAC CAGAGAAGCT AATGCAGAAA ACTCAACAGT ACCATAAGGA AAGAGAACAG 
CCACCACGGT TTGCTCAACC TGGGACATTT GAATTTGAAT ATGCGTCTCG ATGGAAGGCT 
CTTGATGAAA TGGAAAAGCA GCAGCGGGAA CAAGTTGATA GAAACATCAG AGAAGCCAAA 
GAGAAACTGG AGGCAGAGAT GGAAGCAGCT AGACATGAAC ACCAGTTAAT GCTAATGAGG 
CAAGATCTAA TGAGACGTCA AGAAGAACTC AGACGTTTGG AAGAACTCAG AAATCAAGAA 
CTGCAGAAAC GAAAGCAAAT ACAACTGAGA CACGAAGAAG AGCATCGTCG TCGTGAAGAA 
GAAATGATCC GTCACAGAGA GCAGGAGGAG CTGAGGCGAC AGCAAGAGGG CGGCTTTAAA 
CCAAACTATA TGGAAAATAG AGAACAGGAA ATGAGAATGG GTGATATGGG TCCCCGTGGA 
GCAATAAACA TGGGAGATGC ATTTAGCCCA GCACCTGCTG GTACCCAAGG TCCTCCTCCA 
ATGATGGGTA TGAATATGAA CAACAGAGGA ACTATACCTG GCCCACCAAT GGGTCCTGGT 
CCTGCCATGG GACCAGAAGG AGCTGCAAAT ATGGGAACTC CAATGATACC AGATAATGGC 
GCAGTGCACA ATGATAGGTT TCCCCAAGGA CCTCCATCCC AGATGGGTTC ACCCATGGGA 
AATAGAACAG GTTCTGAAAC TCCCCAGGCA CCGATGAGTG GTGTAGGGCC TGTAAGTGGT 
GGTCCTGGCG GCTTTGGTAG AGGGAGTCAA GGGGGCAATT TTGAAGGCCC TAATAAGCGT 
CGGAGATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pspc1 ( NM_025682.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079958.3 Mus musculus paraspeckle protein 1 (Pspc1), mRNA.

Price & Availability↑

CloneID	OMu00865
Clone ID Related Accession (Same CDS sequence)	NM_025682.4 , NM_025682.3
Accession Version	NM_025682.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-10
Organism	Mus musculus(house mouse)
Product	paraspeckle component 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK017689.1 and AK011272.1. On Mar 21, 2009 this sequence version replaced NM_025682.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK017689.1, BC026772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGATGTTAA GAGGAAACCT GAAGCAAGTA CGTATTGAGA AAAACCCGGC TCGTCTTCGC 
GCCCTGGAGT CGGCGGCCGG AGAGAGCGAA CCGGTGGCCG CGGCGGCCAT GGCGCTGACG 
CTGGCCGGCG AGCAGGCGCC GCCACCCGCA CCTTCCGAGG AGCACCCGGA CGAGGAATTG 
GGCTTCACCA TCGACATCAA GAGTTTCCTC AAGCCGGGCG AGAAGACGTA CACGCAGCGC 
TGCCGCCTCT TCGTGGGTAA CCTGCCCACA GACATCACGG AGGAGGACTT CAAGCGCCTC 
TTTGAGCGCT ACGGAGAGCC CAGCGAGGTC TTCATCAACC GCGACCGAGG CTTCGGCTTC 
ATCCGCCTGG AATCGAGAAC ACTGGCTGAA ATTGCAAAAG CAGAGTTGGA TGGCACCATA 
CTGAAGAGCA GACCTCTCCG AATTCGCTTT GCTACACATG GAGCAGCCCT AACGGTCAAG 
AACCTTTCTC CGGTTGTTTC CAATGAGCTT CTAGAGCAAG CATTTTCCCA GTTTGGCCCA 
GTAGAGAAAG CTGTTGTGGT TGTGGATGAT CGTGGTAGAG CCACAGGAAA AGGTTTTGTA 
GAATTTGCAG CGAAACCTCC TGCACGAAAG GCTCTGGAGA GATGTGGTGA TGGGGCGTTC 
TTGCTAACAA CAACACCTCG TCCAGTCATT GTAGAACCCA TGGAGCAGTT TGATGATGAA 
GATGGTTTAC CAGAGAAGCT AATGCAGAAA ACTCAACAGT ACCATAAGGA AAGAGAACAG 
CCACCACGGT TTGCTCAACC TGGGACATTT GAATTTGAAT ATGCGTCTCG ATGGAAGGCT 
CTTGATGAAA TGGAAAAGCA GCAGCGGGAA CAAGTTGATA GAAACATCAG AGAAGCCAAA 
GAGAAACTGG AGGCAGAGAT GGAAGCAGCT AGACATGAAC ACCAGTTAAT GCTAATGAGG 
CAAGATCTAA TGAGACGTCA AGAAGAACTC AGACGTTTGG AAGAACTCAG AAATCAAGAA 
CTGCAGAAAC GAAAGCAAAT ACAACTGAGA CACGAAGAAG AGCATCGTCG TCGTGAAGAA 
GAAATGATCC GTCACAGAGA GCAGGAGGAG CTGAGGCGAC AGCAAGAGGG CGGCTTTAAA 
CCAAACTATA TGGAAAATAG AGAACAGGAA ATGAGAATGG GTGATATGGG TCCCCGTGGA 
GCAATAAACA TGGGAGATGC ATTTAGCCCA GCACCTGCTG GTACCCAAGG TCCTCCTCCA 
ATGATGGGTA TGAATATGAA CAACAGAGGA ACTATACCTG GCCCACCAAT GGGTCCTGGT 
CCTGCCATGG GACCAGAAGG AGCTGCAAAT ATGGGAACTC CAATGATACC AGATAATGGC 
GCAGTGCACA ATGATAGGTT TCCCCAAGGA CCTCCATCCC AGATGGGTTC ACCCATGGGA 
AATAGAACAG GTTCTGAAAC TCCCCAGGCA CCGATGAGTG GTGTAGGGCC TGTAAGTGGT 
GGTCCTGGCG GCTTTGGTAG AGGGAGTCAA GGGGGCAATT TTGAAGGCCC TAATAAGCGT 
CGGAGATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079958.3
CDS	158..1729
Translation	MMLRGNLKQVRIEKNPARLRALESAAGESEPVAAAAMALTLAGEQAPPPAPSEEHPDEELGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGTQGPPPMMGMNMNNRGTIPGPPMGPGPAMGPEGAANMGTPMIPDNGAVHNDRFPQGPPSQMGSPMGNRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY

Target ORF information:

RefSeq Version	NM_025682.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus paraspeckle protein 1 (Pspc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025682.3

ORF Insert Sequence:

ATGATGTTAA GAGGAAACCT GAAGCAAGTA CGTATTGAGA AAAACCCGGC TCGTCTTCGC 
GCCCTGGAGT CGGCGGCCGG AGAGAGCGAA CCGGTGGCCG CGGCGGCCAT GGCGCTGACG 
CTGGCCGGCG AGCAGGCGCC GCCACCCGCA CCTTCCGAGG AGCACCCGGA CGAGGAATTG 
GGCTTCACCA TCGACATCAA GAGTTTCCTC AAGCCGGGCG AGAAGACGTA CACGCAGCGC 
TGCCGCCTCT TCGTGGGTAA CCTGCCCACA GACATCACGG AGGAGGACTT CAAGCGCCTC 
TTTGAGCGCT ACGGAGAGCC CAGCGAGGTC TTCATCAACC GCGACCGAGG CTTCGGCTTC 
ATCCGCCTGG AATCGAGAAC ACTGGCTGAA ATTGCAAAAG CAGAGTTGGA TGGCACCATA 
CTGAAGAGCA GACCTCTCCG AATTCGCTTT GCTACACATG GAGCAGCCCT AACGGTCAAG 
AACCTTTCTC CGGTTGTTTC CAATGAGCTT CTAGAGCAAG CATTTTCCCA GTTTGGCCCA 
GTAGAGAAAG CTGTTGTGGT TGTGGATGAT CGTGGTAGAG CCACAGGAAA AGGTTTTGTA 
GAATTTGCAG CGAAACCTCC TGCACGAAAG GCTCTGGAGA GATGTGGTGA TGGGGCGTTC 
TTGCTAACAA CAACACCTCG TCCAGTCATT GTAGAACCCA TGGAGCAGTT TGATGATGAA 
GATGGTTTAC CAGAGAAGCT AATGCAGAAA ACTCAACAGT ACCATAAGGA AAGAGAACAG 
CCACCACGGT TTGCTCAACC TGGGACATTT GAATTTGAAT ATGCGTCTCG ATGGAAGGCT 
CTTGATGAAA TGGAAAAGCA GCAGCGGGAA CAAGTTGATA GAAACATCAG AGAAGCCAAA 
GAGAAACTGG AGGCAGAGAT GGAAGCAGCT AGACATGAAC ACCAGTTAAT GCTAATGAGG 
CAAGATCTAA TGAGACGTCA AGAAGAACTC AGACGTTTGG AAGAACTCAG AAATCAAGAA 
CTGCAGAAAC GAAAGCAAAT ACAACTGAGA CACGAAGAAG AGCATCGTCG TCGTGAAGAA 
GAAATGATCC GTCACAGAGA GCAGGAGGAG CTGAGGCGAC AGCAAGAGGG CGGCTTTAAA 
CCAAACTATA TGGAAAATAG AGAACAGGAA ATGAGAATGG GTGATATGGG TCCCCGTGGA 
GCAATAAACA TGGGAGATGC ATTTAGCCCA GCACCTGCTG GTACCCAAGG TCCTCCTCCA 
ATGATGGGTA TGAATATGAA CAACAGAGGA ACTATACCTG GCCCACCAAT GGGTCCTGGT 
CCTGCCATGG GACCAGAAGG AGCTGCAAAT ATGGGAACTC CAATGATACC AGATAATGGC 
GCAGTGCACA ATGATAGGTT TCCCCAAGGA CCTCCATCCC AGATGGGTTC ACCCATGGGA 
AATAGAACAG GTTCTGAAAC TCCCCAGGCA CCGATGAGTG GTGTAGGGCC TGTAAGTGGT 
GGTCCTGGCG GCTTTGGTAG AGGGAGTCAA GGGGGCAATT TTGAAGGCCC TAATAAGCGT 
CGGAGATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.
The Journal of clinical investigation127(3)987-1004(2017 Mar)
Wang J,Rajbhandari P,Damianov A,Han A,Sallam T,Waki H,Villanueva CJ,Lee SD,Nielsen R,Mandrup S,Reue K,Young SG,Whitelegge J,Saez E,Black DL,Tontonoz P

Synergistic upregulation of NONO and PSPC1 regulates Sertoli cell response to MEHP via modulation of ALDH1A1 signaling.
FEBS letters591(6)914-923(2017 Mar)
Dong BW,Jin XH,Yan CY,Yang T,Cai GQ,Lu J

Specific interaction with the nuclear transporter importin ?2 can modulate paraspeckle protein 1 delivery to nuclear paraspeckles.
Molecular biology of the cell26(8)1543-58(2015 Apr)
Major AT,Hogarth CA,Miyamoto Y,Sarraj MA,Smith CL,Koopman P,Kurihara Y,Jans DA,Loveland KL

Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog.
Nucleic acids research42(15)9771-80(2014 Sep)
Li S,Li Z,Shu FJ,Xiong H,Phillips AC,Dynan WS

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Pspc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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