Srp54b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srp54b
Entrez Gene ID	665155
Full Name	signal recognition particle 54B
General protein information	Preferred Names signal recognition particle 54B Names signal recognition particle 54 kDa protein SRP54
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006516155.3	XP_006516218.1	signal recognition particle 54 kDa protein isoform X1
NM_001100109.1	NP_001093579.1	signal recognition particle 54 kDa protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Srp54a	NP_036029.2
Danio rerio (zebrafish)	srp54	NP_957282.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004610	XP_315212.2
Caenorhabditis elegans (roundworm)	F21D5.7	NP_501507.1
Saccharomyces cerevisiae (baker's yeast)	SRP54	NP_015413.1
Kluyveromyces lactis	KLLA0D09020g	XP_453464.1
Homo sapiens (human)	SRP54	NP_003127.1
Rattus norvegicus (Norway rat)	Srp54a	NP_446323.1
Magnaporthe oryzae (rice blast fungus)	MGG_05977	XP_003711822.1
Arabidopsis thaliana (thale cress)	AT1G48900	NP_564535.1
Canis lupus familiaris (dog)	SRP54	NP_001003272.1
Bos taurus (cattle)	SRP54	NP_001033290.1
Gallus gallus (chicken)	SRP54	XP_421238.1
Schizosaccharomyces pombe (fission yeast)	srp54	NP_588209.1
Neurospora crassa	NCU09696	XP_960643.1
Oryza sativa (rice)	Os01g0772800	NP_001044395.1
Pan troglodytes (chimpanzee)	SRP54	XP_001136048.1
	SRP54	XP_001082531.1
Mus musculus (house mouse)	Srp54b	NP_001093579.1
Drosophila melanogaster (fruit fly)	Srp54k	NP_523931.1
Eremothecium gossypii	AGOS_AAR041W	NP_982582.2
Xenopus tropicalis (tropical clawed frog)	srp54	NP_988977.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the signal recognition particle interacting with the elongation-arrested ribosome.
Halic M, Becker T, Pool MR, Spahn CM, Grassucci RA, Frank J, Beckmann R
Nature427(6977)808-14(2004 Feb)

The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence.
Zopf D, Bernstein HD, Johnson AE, Walter P
The EMBO journal9(13)4511-7(1990 Dec)

Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle.
Bernstein HD, Poritz MA, Strub K, Hoben PJ, Brenner S, Walter P
Nature340(6233)482-6(1989 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Srp54b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srp54b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03443	NM_001100109.1 Latest version!	Mus musculus signal recognition particle 54B (Srp54b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu47842	XM_006516155.3 Latest version!	Mus musculus signal recognition particle 54B (Srp54b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srp54b ( NM_001100109.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001093579.1 Mus musculus signal recognition particle 54B (Srp54b), mRNA.

Price & Availability↑

CloneID	OMu03443
Clone ID Related Accession (Same CDS sequence)	NM_001100109.1
Accession Version	NM_001100109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	signal recognition particle 54 kDa protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK136033.1. On or before Jul 26, 2007 this sequence version replaced XM_001479059.1, XM_976391.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.54090.1, SRR1660819.152885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTTCTAG CAGATCTTGG AAGAAAAATA ACATCAGCAT TACGCTCATT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTAAATGCT ATGCTGAAAG AAGTATGTAC AGCATTATTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTCAGAG AAAATGTTAA GTCTGCAATT 
GATCTTGAAG AGATGGCATC TGGACTGAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TAAAGCTTGT AGACCCTGGA GTTAAAGCGT GGACACCGAC TAAGGGAAAG 
CAAAATGTGA TCATGTTTGT TGGATTGCAA GGGAGTGGTA AAACGACAAC ATGTTCAAAG 
TTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TGATATGTGC AGATACATTC 
AGAGCAGGAG CCTTTGACCA GCTAAAACAG AATGCCACCA AAGCAAGAAT TCCGTTCTAT 
GGCAGCTATA CTGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTGGA GAAATTCAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGTC GTCACAAACA AGAAGACTCT 
TTATTTGAAG AAATGCTTCA AGTTTCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCAT CCATCGGACA GGCTTGTGAG GCCCAGGCGA AGGCCTTTAA AGACAAAGTA 
GATGTAGCTT CAGTAATAGT GACAAAACTC GACGGTCATG CGAAAGGAGG CGGTGCTCTT 
AGTGCAGTTG CTGCCACAAA AAGTCCAATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GATTTTGAAC CTTTCAAAAC ACAACCTTTC ATCAGCAAAC TCCTTGGAAT GGGTGATATT 
GAAGGACTGA TTGATAAAGT CAATGAATTG AAGTTGGATG ATAATGAGGC ACTTATAGAG 
AAGTTGAAGC ACGGTCAGTT TACATTGCGA GACATGTATG AACAGTTTCA GAATATTATG 
AAAATGGGCC CATTCAGTCA GATATTGGGG ATGATTCCTG GCTTTGGCAC AGATTTTATG 
AGCAAAGGGA ATGAGCAGGA GTCAATGGCA AGGCTGAAGA AACTGATGAC AATCATGGAC 
AGTATGAACG ATCAAGAACT GGACAGTACA GATGGTGCCA AGGTTTTCAG TAAGCAACCA 
GGGAGAATCC AAAGAGTTGC CCGGGGATCA GGTGTGTCAA CAAGAGATGT TCAAGAACTT 
CTGACCCAGT ATACCAAGTT TGCACAGATG GTCAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGCG GTGATATGTC TAAGAATGTG AGTCAGTCAC AGATGGCAAA ATTAAACCAA 
CAAATGGCCA AAATGATGGA CCCACGAGTT CTTCATCACA TGGGTGGCAT GGCCGGCCTT 
CAGTCAATGA TGCGGCAGTT TCAGCAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAACA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093579.1
CDS	510..2024
Translation	MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVSNAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	NM_001100109.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus signal recognition particle 54B (Srp54b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100109.1

ORF Insert Sequence:

ATGGTTCTAG CAGATCTTGG AAGAAAAATA ACATCAGCAT TACGCTCATT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTAAATGCT ATGCTGAAAG AAGTATGTAC AGCATTATTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTCAGAG AAAATGTTAA GTCTGCAATT 
GATCTTGAAG AGATGGCATC TGGACTGAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TAAAGCTTGT AGACCCTGGA GTTAAAGCGT GGACACCGAC TAAGGGAAAG 
CAAAATGTGA TCATGTTTGT TGGATTGCAA GGGAGTGGTA AAACGACAAC ATGTTCAAAG 
TTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TGATATGTGC AGATACATTC 
AGAGCAGGAG CCTTTGACCA GCTAAAACAG AATGCCACCA AAGCAAGAAT TCCGTTCTAT 
GGCAGCTATA CTGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTGGA GAAATTCAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGTC GTCACAAACA AGAAGACTCT 
TTATTTGAAG AAATGCTTCA AGTTTCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCAT CCATCGGACA GGCTTGTGAG GCCCAGGCGA AGGCCTTTAA AGACAAAGTA 
GATGTAGCTT CAGTAATAGT GACAAAACTC GACGGTCATG CGAAAGGAGG CGGTGCTCTT 
AGTGCAGTTG CTGCCACAAA AAGTCCAATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GATTTTGAAC CTTTCAAAAC ACAACCTTTC ATCAGCAAAC TCCTTGGAAT GGGTGATATT 
GAAGGACTGA TTGATAAAGT CAATGAATTG AAGTTGGATG ATAATGAGGC ACTTATAGAG 
AAGTTGAAGC ACGGTCAGTT TACATTGCGA GACATGTATG AACAGTTTCA GAATATTATG 
AAAATGGGCC CATTCAGTCA GATATTGGGG ATGATTCCTG GCTTTGGCAC AGATTTTATG 
AGCAAAGGGA ATGAGCAGGA GTCAATGGCA AGGCTGAAGA AACTGATGAC AATCATGGAC 
AGTATGAACG ATCAAGAACT GGACAGTACA GATGGTGCCA AGGTTTTCAG TAAGCAACCA 
GGGAGAATCC AAAGAGTTGC CCGGGGATCA GGTGTGTCAA CAAGAGATGT TCAAGAACTT 
CTGACCCAGT ATACCAAGTT TGCACAGATG GTCAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGCG GTGATATGTC TAAGAATGTG AGTCAGTCAC AGATGGCAAA ATTAAACCAA 
CAAATGGCCA AAATGATGGA CCCACGAGTT CTTCATCACA TGGGTGGCAT GGCCGGCCTT 
CAGTCAATGA TGCGGCAGTT TCAGCAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAACA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srp54b ( XM_006516155.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516218.1 Mus musculus signal recognition particle 54B (Srp54b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47842
Clone ID Related Accession (Same CDS sequence)	XM_006516155.3
Accession Version	XM_006516155.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	signal recognition particle 54 kDa protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516155.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAAG GAGCCTTTGA CCAGCTAAAA CAGAATGCCA CCAAAGCAAG AATTCCGTTC 
TATGGCAGCT ATACTGAAAT GGATCCTGTC ATCATTGCTT CTGAAGGAGT GGAGAAATTC 
AAAAATGAAA ATTTTGAAAT TATTATTGTT GATACAAGTG GTCGTCACAA ACAAGAAGAC 
TCTTTATTTG AAGAAATGCT TCAAGTTTCT AATGCTATAC AACCTGATAA CATTGTTTAT 
GTGATGGATG CATCCATCGG ACAGGCTTGT GAGGCCCAGG CGAAGGCCTT TAAAGACAAA 
GTAGATGTAG CTTCAGTAAT AGTGACAAAA CTCGACGGTC ATGCGAAAGG AGGCGGTGCT 
CTTAGTGCAG TTGCTGCCAC AAAAAGTCCA ATTATTTTCA TTGGTACAGG GGAACATATA 
GATGATTTTG AACCTTTCAA AACACAACCT TTCATCAGCA AACTCCTTGG AATGGGTGAT 
ATTGAAGGAC TGATTGATAA AGTCAATGAA TTGAAGTTGG ATGATAATGA GGCACTTATA 
GAGAAGTTGA AGCACGGTCA GTTTACATTG CGAGACATGT ATGAACAGTT TCAGAATATT 
ATGAAAATGG GCCCATTCAG TCAGATATTG GGGATGATTC CTGGCTTTGG CACAGATTTT 
ATGAGCAAAG GGAATGAGCA GGAGTCAATG GCAAGGCTGA AGAAACTGAT GACAATCATG 
GACAGTATGA ACGATCAAGA ACTGGACAGT ACAGATGGTG CCAAGGTTTT CAGTAAGCAA 
CCAGGGAGAA TCCAAAGAGT TGCCCGGGGA TCAGGTGTGT CAACAAGAGA TGTTCAAGAA 
CTTCTGACCC AGTATACCAA GTTTGCACAG ATGGTCAAAA AGATGGGAGG TATCAAAGGA 
CTTTTCAAAG GCGGTGATAT GTCTAAGAAT GTGAGTCAGT CACAGATGGC AAAATTAAAC 
CAACAAATGG CCAAAATGAT GGACCCACGA GTTCTTCATC ACATGGGTGG CATGGCCGGC 
CTTCAGTCAA TGATGCGGCA GTTTCAGCAG GGTGCTGCTG GCAACATGAA AGGCATGATG 
GGATTCAATA ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516218.1
CDS	85..1182
Translation	MFKGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVSNAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	XM_006516155.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus signal recognition particle 54B (Srp54b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516155.3

ORF Insert Sequence:

ATGTTCAAAG GAGCCTTTGA CCAGCTAAAA CAGAATGCCA CCAAAGCAAG AATTCCGTTC 
TATGGCAGCT ATACTGAAAT GGATCCTGTC ATCATTGCTT CTGAAGGAGT GGAGAAATTC 
AAAAATGAAA ATTTTGAAAT TATTATTGTT GATACAAGTG GTCGTCACAA ACAAGAAGAC 
TCTTTATTTG AAGAAATGCT TCAAGTTTCT AATGCTATAC AACCTGATAA CATTGTTTAT 
GTGATGGATG CATCCATCGG ACAGGCTTGT GAGGCCCAGG CGAAGGCCTT TAAAGACAAA 
GTAGATGTAG CTTCAGTAAT AGTGACAAAA CTCGACGGTC ATGCGAAAGG AGGCGGTGCT 
CTTAGTGCAG TTGCTGCCAC AAAAAGTCCA ATTATTTTCA TTGGTACAGG GGAACATATA 
GATGATTTTG AACCTTTCAA AACACAACCT TTCATCAGCA AACTCCTTGG AATGGGTGAT 
ATTGAAGGAC TGATTGATAA AGTCAATGAA TTGAAGTTGG ATGATAATGA GGCACTTATA 
GAGAAGTTGA AGCACGGTCA GTTTACATTG CGAGACATGT ATGAACAGTT TCAGAATATT 
ATGAAAATGG GCCCATTCAG TCAGATATTG GGGATGATTC CTGGCTTTGG CACAGATTTT 
ATGAGCAAAG GGAATGAGCA GGAGTCAATG GCAAGGCTGA AGAAACTGAT GACAATCATG 
GACAGTATGA ACGATCAAGA ACTGGACAGT ACAGATGGTG CCAAGGTTTT CAGTAAGCAA 
CCAGGGAGAA TCCAAAGAGT TGCCCGGGGA TCAGGTGTGT CAACAAGAGA TGTTCAAGAA 
CTTCTGACCC AGTATACCAA GTTTGCACAG ATGGTCAAAA AGATGGGAGG TATCAAAGGA 
CTTTTCAAAG GCGGTGATAT GTCTAAGAAT GTGAGTCAGT CACAGATGGC AAAATTAAAC 
CAACAAATGG CCAAAATGAT GGACCCACGA GTTCTTCATC ACATGGGTGG CATGGCCGGC 
CTTCAGTCAA TGATGCGGCA GTTTCAGCAG GGTGCTGCTG GCAACATGAA AGGCATGATG 
GGATTCAATA ACATGTAA