Gkn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gkn2
Entrez Gene ID	66284
Full Name	gastrokine 2
Synonyms	1810036H07Rik,Bricd1b
General protein information	Preferred Names gastrokine 2 Names gastrokine-2 blottin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025467.1	NP_079743.1	gastrokine-2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GKN2	XP_003309113.1
Bos taurus (cattle)	GKN2	XP_002691485.1
Rattus norvegicus (Norway rat)	Gkn2	NP_001034775.1
Homo sapiens (human)	GKN2	NP_872342.2
	GKN2	XP_001096536.1
Gallus gallus (chicken)	GKN2	XP_417666.1
Mus musculus (house mouse)	Gkn2	NP_079743.1
Canis lupus familiaris (dog)	GKN2	XP_855420.2

Gene Ontology
Bibliography

Related articles in PubMed

The Rodent-Specific MicroRNA Cluster within the Sfmbt2 Gene Is Imprinted and Essential for Placental Development.
Inoue K, Hirose M, Inoue H, Hatanaka Y, Honda A, Hasegawa A, Mochida K, Ogura A
Cell reports19(5)949-956(2017 May)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Loss of gastrokine-2 drives premalignant gastric inflammation and tumor progression.
Menheniott TR, O'Connor L, Chionh YT, D?britz J, Scurr M, Rollo BN, Ng GZ, Jacobs S, Catubig A, Kurklu B, Mercer S, Minamoto T, Ong DE, Ferrero RL, Fox JG, Wang TC, Sutton P, Judd LM, Giraud AS
The Journal of clinical investigation126(4)1383-400(2016 Apr)

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
West DB, Engelhard EK, Adkisson M, Nava AJ, Kirov JV, Cipollone A, Willis B, Rapp J, de Jong PJ, Lloyd KC
PLoS genetics12(2)e1005691(2016 Feb)

Gastrokine-2 is transiently expressed in the endodermal and endothelial cells of the maturing mouse yolk sac.
Antas VI, Brigden KW, Prudence AJ, Fraser ST
Gene expression patterns : GEP16(2)69-74(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results reveal an antiinflammatory role for GKN2, provide in vivo evidence that links GKN2 loss to GC pathogenesis, and suggest GKN restoration as a strategy to restrain GC progression.
Title: Loss of gastrokine-2 drives premalignant gastric inflammation and tumor progression.

Yolk sac endothelial cells were found to express low levels of Gkn2 transcript and protein.
Title: Gastrokine-2 is transiently expressed in the endodermal and endothelial cells of the maturing mouse yolk sac.

The following Gkn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gkn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00534	NM_025467.1 Latest version!	Mus musculus gastrokine 2 (Gkn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gkn2 ( NM_025467.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079743.1 Mus musculus gastrokine 2 (Gkn2), mRNA.

Price & Availability↑

CloneID	OMu00534
Clone ID Related Accession (Same CDS sequence)	NM_025467.1
Accession Version	NM_025467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	gastrokine-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK007705.1. ##Evidence-Data-START## Transcript exon combination :: AK007705.1, AY943909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGAAACCCC TCGTGGCATT TCTGGTGGTG CTGTCCATCT TTGGAATACA ATCGCAGGCA 
GAGGAGATTT TTAATATCTT CGTCCCAAGC AAAAATGGAG GCAATATCCA GGAGACAGTG 
ACCATCGACA ACCAGCAGAA CACGGCCACC ATCAACATCC ACTCAGGCTC GTGCTCCTCC 
ACCACGATTT TTGACTATAA GCATGGTTAC ATCGCATCCC GGGTGCTCTC CCGGAGAGCC 
TGTTACGTCA TCAAGATGGA CCACAAAGCC ATCCCTGCTC TGGACAAACT CCAACGGTTC 
CTCTACGAGA AGCAGACAAT GAATGCTATC GACTCCCCAG AGTATACCTG GGTCAGGTAC 
AACCCTCTGA AGTCTCTGAT CACGAAGGTG GACTGGTTCC TGTTTGGGTC GCCTATTCGA 
CAGCTATGCA AGCACATGCC CCTGTATGAG GGTGAGGTGG CTACAAAGCC AAAAGAGGTG 
AGCACTGGAG CCTGTGCAAA GGTTGGACTC CTGGGGATCC TGGGAGTCTC CATCTGTGGA 
GGAATTCACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079743.1
CDS	32..586
Translation	MKPLVAFLVVLSIFGIQSQAEEIFNIFVPSKNGGNIQETVTIDNQQNTATINIHSGSCSSTTIFDYKHGYIASRVLSRRACYVIKMDHKAIPALDKLQRFLYEKQTMNAIDSPEYTWVRYNPLKSLITKVDWFLFGSPIRQLCKHMPLYEGEVATKPKEVSTGACAKVGLLGILGVSICGGIHL

Target ORF information:

RefSeq Version	NM_025467.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gastrokine 2 (Gkn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025467.1

ORF Insert Sequence:

ATGAAACCCC TCGTGGCATT TCTGGTGGTG CTGTCCATCT TTGGAATACA ATCGCAGGCA 
GAGGAGATTT TTAATATCTT CGTCCCAAGC AAAAATGGAG GCAATATCCA GGAGACAGTG 
ACCATCGACA ACCAGCAGAA CACGGCCACC ATCAACATCC ACTCAGGCTC GTGCTCCTCC 
ACCACGATTT TTGACTATAA GCATGGTTAC ATCGCATCCC GGGTGCTCTC CCGGAGAGCC 
TGTTACGTCA TCAAGATGGA CCACAAAGCC ATCCCTGCTC TGGACAAACT CCAACGGTTC 
CTCTACGAGA AGCAGACAAT GAATGCTATC GACTCCCCAG AGTATACCTG GGTCAGGTAC 
AACCCTCTGA AGTCTCTGAT CACGAAGGTG GACTGGTTCC TGTTTGGGTC GCCTATTCGA 
CAGCTATGCA AGCACATGCC CCTGTATGAG GGTGAGGTGG CTACAAAGCC AAAAGAGGTG 
AGCACTGGAG CCTGTGCAAA GGTTGGACTC CTGGGGATCC TGGGAGTCTC CATCTGTGGA 
GGAATTCACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Rodent-Specific MicroRNA Cluster within the Sfmbt2 Gene Is Imprinted and Essential for Placental Development.
Cell reports19(5)949-956(2017 May)
Inoue K,Hirose M,Inoue H,Hatanaka Y,Honda A,Hasegawa A,Mochida K,Ogura A

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Loss of gastrokine-2 drives premalignant gastric inflammation and tumor progression.
The Journal of clinical investigation126(4)1383-400(2016 Apr)
Menheniott TR,O'Connor L,Chionh YT,D?britz J,Scurr M,Rollo BN,Ng GZ,Jacobs S,Catubig A,Kurklu B,Mercer S,Minamoto T,Ong DE,Ferrero RL,Fox JG,Wang TC,Sutton P,Judd LM,Giraud AS

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
PLoS genetics12(2)e1005691(2016 Feb)
West DB,Engelhard EK,Adkisson M,Nava AJ,Kirov JV,Cipollone A,Willis B,Rapp J,de Jong PJ,Lloyd KC

Gastrokine-2 is transiently expressed in the endodermal and endothelial cells of the maturing mouse yolk sac.
Gene expression patterns : GEP16(2)69-74(2014 Nov)
Antas VI,Brigden KW,Prudence AJ,Fraser ST