Piwil1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Piwil1
Entrez Gene ID	57749
Full Name	piwi-like RNA-mediated gene silencing 1
Synonyms	MIWI
General protein information	Preferred Names piwi-like RNA-mediated gene silencing 1 Names piwi-like protein 1 piwi-like homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 G1.3\|5 67.86 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006504319.2	XP_006504382.1	piwi-like protein 1 isoform X1
XM_006504318.1	XP_006504381.1	piwi-like protein 1 isoform X1
XM_006504318.2	XP_006504381.1	piwi-like protein 1 isoform X1
NM_021311.3	NP_067286.1	piwi-like protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIWIL1	NP_004755.2
Pan troglodytes (chimpanzee)	PIWIL1	XP_001137913.1
Xenopus tropicalis (tropical clawed frog)	LOC100496286	XP_002942570.2
Canis lupus familiaris (dog)	PIWIL1	XP_534638.2
Mus musculus (house mouse)	Piwil1	NP_067286.1
Rattus norvegicus (Norway rat)	Piwil1	NP_001102323.1
Danio rerio (zebrafish)	piwil1	NP_899181.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011204	XP_564296.2
Caenorhabditis elegans (roundworm)	prg-1	NP_492121.1
Caenorhabditis elegans (roundworm)	prg-2	NP_500994.1
Bos taurus (cattle)	PIWIL1	XP_002694505.2
Gallus gallus (chicken)	PIWIL1	NP_001092322.1
Drosophila melanogaster (fruit fly)	aub	NP_476734.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009509	XP_310187.3
Xenopus tropicalis (tropical clawed frog)	piwil1	XP_002940227.2

Gene Ontology
Bibliography

Related articles in PubMed

MORC2B is essential for meiotic progression and fertility.
Shi B, Xue J, Zhou J, Kasowitz SD, Zhang Y, Liang G, Guan Y, Shi Q, Liu M, Sha J, Huang X, Wang PJ
PLoS genetics14(1)e1007175(2018 Jan)

TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.
Ding D, Liu J, Midic U, Wu Y, Dong K, Melnick A, Latham KE, Chen C
Nature communications9(1)127(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MIWI (or PIWIL1) depletion had a smaller effect. In oocytes lacking PLD6 (or ZUCCHINI or MITOPLD), a mitochondrial nuclease/phospholipase involved in piRNA biogenesis in male germ cells, the piRNA level was decreased to 50% compared to wild-type.
Title: Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes.

piRNA biogenesis triggered by PIWI slicing, and promoted by EXD1, ensures that the same guides instruct PIWI proteins in the nucleus and cytoplasm.
Title: PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway.

Stem cell protein Piwil1 plays an important role in regulating stem-like properties of endometrial cancer cells.
Title: Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition.

PIWIL1 regulates neuronal polarization and radial migration partly via modulating the expression of microtubule-associated proteins.
Title: Novel function of PIWIL1 in neuronal polarization and migration via regulation of microtubule-associated proteins.

Results show that HIWI is highly expressed in high-grade squamous intraepithelial lesions and cervical cancer tissues suggesting its participation in the carcinogenesis of cervical cancer.
Title: Hiwi facilitates chemoresistance as a cancer stem cell marker in cervical cancer.

The following Piwil1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piwil1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19615	NM_021311.3 Latest version!	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu19615	XM_006504318.2 Latest version!	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu19615	XM_006504319.2 Latest version!	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
View Old Accession Versions >>
OMu19615	XM_006504318.1	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Piwil1 ( NM_021311.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067286.1 Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), mRNA.

Price & Availability↑

CloneID	OMu19615
Clone ID Related Accession (Same CDS sequence)	XM_006504319.2 , XM_006504318.1 , XM_006504318.2 , NM_021311.3
Accession Version	NM_021311.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	piwi-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC111089.27. On Nov 17, 2006 this sequence version replaced NM_021311.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF438405.1, AB032604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067286.1
CDS	191..2779
Translation	MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL

Target ORF information:

RefSeq Version	NM_021311.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021311.3

ORF Insert Sequence:

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piwil1 ( XM_006504319.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504382.1 Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu19615
Clone ID Related Accession (Same CDS sequence)	XM_006504319.2 , XM_006504318.1 , XM_006504318.2 , NM_021311.3
Accession Version	XM_006504319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	piwi-like protein 1 isoform X1
Comment

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006504382.1

Translation

MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL

Target ORF information:

RefSeq Version	XM_006504319.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504319.2

ORF Insert Sequence:

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piwil1 ( XM_006504318.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504381.1 Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19615
Clone ID Related Accession (Same CDS sequence)	XM_006504319.2 , XM_006504318.1 , XM_006504318.2 , NM_021311.3
Accession Version	XM_006504318.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	piwi-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504381.1
CDS	133..2721
Translation	MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL

Target ORF information:

RefSeq Version	XM_006504318.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504318.1

ORF Insert Sequence:

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piwil1 ( XM_006504318.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504381.1 Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19615
Clone ID Related Accession (Same CDS sequence)	XM_006504319.2 , XM_006504318.1 , XM_006504318.2 , NM_021311.3
Accession Version	XM_006504318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	piwi-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504381.1
CDS	132..2720
Translation	MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL

Target ORF information:

RefSeq Version	XM_006504318.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504318.2

ORF Insert Sequence:

ATGACTGGCC GAGCCCGAGC TCGGGCCCGC GGCAGGGCAC GAGGTCAGGA GACGGTGCAG 
CATGTTGGGG CTGCTGCGAG CCAGCAACCT GGGTACATCC CACCGAGACC TCAACAGTCC 
CCCACAGAGG GGGACTTGGT TGGCCGAGGA CGACAGAGGG GGATGGTAGT CGGAGCCACA 
TCCAAGTCAC AAGAACTGCA GATCTCAGCT GGGTTTCAGG AGCTGTCACT GGCAGAGAGA 
GGAGGGCGTC GCCGAGACTT CCATGACCTT GGTGTGAACA CCAGACAGAA CCTTGACCAT 
GTCAAAGAGT CAAAGACAGG CTCCTCTGGC ATCATTGTGA AGCTGAGCAC CAACCACTTC 
CGGCTGACCT CGCGCCCACA GTGGGCCCTG TATCAGTACC ACATCGACTA CAATCCCCTG 
ATGGAGGCCC GAAGGCTTCG CTCCGCACTG CTCTTCCAGC ATGAAGACCT CATTGGAAGG 
TGTCATGCTT TCGATGGGAC AATATTGTTT TTACCTAAGA GACTACAGCA CAAGGTCACA 
GAAGTATTCA GTCAGACTCG GAATGGGGAA CACGTGAGGA TCACCATCAC CCTGACCAAC 
GAGCTGCCGC CCACCTCGCC CACCTGCCTG CAGTTCTATA ACATCATCTT CAGGAGGCTC 
TTGAAAATCA TGAATTTGCA ACAAATTGGA CGGAATTATT ACAATCCAAG TGACCCGATT 
GATATTCCAA ACCACAGGTT GGTGATCTGG CCCGGCTTCA CCACCTCCAT CCTTCAGTAT 
GAGAACAACA TCATGCTCTG CACAGACGTC AGCCACAAGG TGCTCCGCAG CGAGACTGTC 
CTAGACTTCA TGTTCAATCT ATACCAGCAG ACAGAGGAGC ACAAGTTCCA GGAGCAAGTG 
TCGAAGGAGC TCATAGGCCT CATCGTTCTC ACCAAGTACA ATAACAAGAC CTACCGGGTG 
GATGACATTG ACTGGGACCA GAATCCAAAG AGCACCTTCA AGAAGGCGGA TGGCTCGGAG 
GTCAGCTTCC TGGAGTACTA CAGGAAGCAA TACAACCAGG AGATCACGGA CCTGAAGCAG 
CCGGTGCTGG TGAGCCAACC CAAGCGGAGG AGAGGCCCCG GCGGCACCCT GCCTGGCCCA 
GCTATGCTCA TCCCTGAACT CTGCTATCTC ACAGGCCTGA CTGATAAAAT GCGCAATGAT 
TTCAATGTGA TGAAGGACCT GGCAGTGCAC ACGCGGCTGA CCCCTGAGCA GCGGCAGCGG 
GAGGTGGGCC GCCTCATCGA CTACATCCAC AAGGATGACA ATGTGCAGAG AGAGCTTCGA 
GACTGGGGCC TGAGCTTCGA CTCAAACTTG CTGTCCTTCT CTGGAAGAAT CTTACAATCT 
GAGAAGATCC ACCAGGGCGG AAAGACGTTT GATTACAACC CACAATTTGC AGACTGGTCC 
AAAGAAACAA GAGGCGCGCC GCTGATCAGC GTGAAGCCAT TGGATAACTG GCTGCTGATC 
TATACCCGCA GGAATTATGA AGCAGCCAAC TCACTGATAC AGAACCTGTT CAAAGTGACT 
CCAGCCATGG GCATCCAGAT GAAAAAGGCA ATCATGATCG AGGTGGATGA CAGAACAGAA 
GCTTATCTGA GAGCCTTGCA GCAGAAGGTG ACGTCAGACA CTCAGATAGT TGTCTGTCTC 
TTGTCAAGTA ATCGGAAGGA CAAATATGAT GCCATCAAGA AGTACTTGTG TACAGACTGC 
CCCACCCCAA GTCAGTGTGT GGTGGCCCGG ACCCTGGGCA AGCAGCAAAC AGTCATGGCC 
ATTGCCACCA AGATCGCCCT GCAGATGAAC TGCAAGATGG GAGGCGAGCT CTGGCGGGTG 
GACATGCCCC TGAAACTGGC AATGATCGTG GGCATCGACT GTTACCATGA CACCACAGCT 
GGGCGGAGGT CCATCGCAGG ATTCGTCGCC AGCATCAATG AAGGGATGAC CCGCTGGTTC 
TCCCGCTGCG TCTTTCAGGA CCGCGGGCAG GAGCTGGTGG ATGGTCTCAA GGTGTGCTTG 
CAAGCTGCTC TGAGGGCTTG GAGTGGCTGC AATGAATACA TGCCCAGCCG TGTCATCGTG 
TACCGAGACG GTGTGGGGGA CGGGCAGCTG AAGACCCTGG TCAATTATGA GGTCCCACAG 
TTCCTAGATT GCCTCAAGTC AGTCGGGAGA GGTTACAACC CAAGACTGAC TGTAATCGTG 
GTGAAGAAGC GTGTCAATGC CAGGTTTTTT GCTCAGTCTG GGGGAAGACT TCAGAACCCT 
CTTCCAGGGA CAGTCATCGA TGTGGAAGTC ACCAGACCAG AGTGGTATGA CTTTTTCATC 
GTGAGCCAGG CAGTGAGAAG CGGGAGTGTG TCCCCAACAC ACTACAATGT CATCTATGAC 
AGCAGTGGCC TGAAGCCCGA CCACATCCAG CGGCTGACAT ACAAGCTCTG CCACGTGTAC 
TATAATTGGC CTGGAGTCAT CCGAGTCCCT GCACCTTGCC AGTATGCACA CAAGCTGGCC 
TTCCTCGTGG GCCAGAGCAT CCACAGAGAG CCAAACCTCT CCCTGTCCAA CCGCCTCTAC 
TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MORC2B is essential for meiotic progression and fertility.
PLoS genetics14(1)e1007175(2018 Jan)
Shi B,Xue J,Zhou J,Kasowitz SD,Zhang Y,Liang G,Guan Y,Shi Q,Liu M,Sha J,Huang X,Wang PJ

TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.
Nature communications9(1)127(2018 Jan)
Ding D,Liu J,Midic U,Wu Y,Dong K,Melnick A,Latham KE,Chen C

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Piwil1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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