Ust cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ust
Entrez Gene ID	338362
Full Name	uronyl-2-sulfotransferase
Synonyms	D930010O20Rik,UA2OST
General protein information	Preferred Names uronyl-2-sulfotransferase Names uronyl 2-sulfotransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_177387.3	NP_796361.2	uronyl 2-sulfotransferase
XM_006512772.3	XP_006512835.1	uronyl 2-sulfotransferase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UST	NP_005706.1
Bos taurus (cattle)	UST	NP_001193756.1
Pan troglodytes (chimpanzee)	UST	XP_003950984.1
Mus musculus (house mouse)	Ust	NP_796361.2
Xenopus tropicalis (tropical clawed frog)	ust	XP_002936303.1
	UST	XP_001085249.1
Rattus norvegicus (Norway rat)	Ust	NP_001101928.1
Gallus gallus (chicken)	UST	XP_001231938.3
Canis lupus familiaris (dog)	UST	XP_005615558.1
Danio rerio (zebrafish)	ust	NP_001027901.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity.
Paul A, Crow M, Raudales R, He M, Gillis J, Huang ZJ
Cell171(3)522-539.e20(2017 Oct)

Melanoma Cell Adhesion and Migration Is Modulated by the Uronyl 2-O Sulfotransferase.
Nikolovska K, Spillmann D, Haier J, Lad?nyi A, Stock C, Seidler DG
PloS one12(1)e0170054(2017)

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Lewandowski JP, Du F, Zhang S, Powell MB, Falkenstein KN, Ji H, Vokes SA
Developmental biology406(1)92-103(2015 Oct)

Opposing functions of chondroitin sulfate and heparan sulfate during early neuronal polarization.
Nishimura K, Ishii M, Kuraoka M, Kamimura K, Maeda N
Neuroscience169(4)1535-47(2010 Sep)

Oversulfated chondroitin sulfate plays critical roles in the neuronal migration in the cerebral cortex.
Ishii M, Maeda N
The Journal of biological chemistry283(47)32610-20(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first study showing that Ust, and consequently 2-O sulfation of the low affinity receptor for FgfR CS/DS, reduces Itga5 and leads to an impaired adhesion and migration of melanoma cells
Title: Melanoma Cell Adhesion and Migration Is Modulated by the Uronyl 2-O Sulfotransferase.

analysis of the three CS sulfotransferases in the developing neocortex: uronyl 2-O-sulfotransferase (UST), N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (4,6-ST) and dermatan 4-O-sulfotransferase-1 (D4-ST)
Title: Oversulfated chondroitin sulfate plays critical roles in the neuronal migration in the cerebral cortex.

The following Ust gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ust cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06377	NM_177387.3 Latest version!	Mus musculus uronyl-2-sulfotransferase (Ust), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu43900	XM_006512772.3 Latest version!	Mus musculus uronyl-2-sulfotransferase (Ust), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ust ( NM_177387.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796361.2 Mus musculus uronyl-2-sulfotransferase (Ust), mRNA.

Price & Availability↑

CloneID	OMu06377
Clone ID Related Accession (Same CDS sequence)	NM_177387.3
Accession Version	NM_177387.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	uronyl 2-sulfotransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147850.1, AK162950.1 and AC159466.3. On Jul 19, 2006 this sequence version replaced NM_177387.2. ##Evidence-Data-START## Transcript exon combination :: AK147850.1, SRR1660815.263651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAGAAGA AGCAGCAGCA GCATCCCGGC GGCGGCACGG ATCCCTGGCC CCATGGGGCC 
CCTGTGGGGG GCGCCCCTCC GTGCCTGGGC AGCTGCAAGC GCCGGATCCC GCTGCTGCCT 
TTCCTGCGCT TCTCCCTCCG GGACTACGGT TTCTGCATGG CTACCCTGCT GGTCTTCTGC 
CTGGGCTCCC TCTTCTACCA GCTCAGCGGG GGACCCCCTC GCTTCCTGCT CGACCTGCGG 
CAGTATTTGG GAAATTCCAC TTACTTGGAT GACCATGGAC CACCTCCTAG TAAGGTCCTT 
CCCTTCCCCA GCCAGGTGGT GTACAACAGA GTTGGCAAGT GTGGCAGTCG TACCGTGGTC 
TTGCTTCTGA GAATCTTGTC AGAGAAGCAC GGCTTCAATT TGGTCACATC AGACATTCAC 
AACAAGACCC GGCTTACTAA AAATGAACAA ATGGAACTGA TTAAGAATAT AAGTACCGCC 
GAACAGCCCT ATTTATTCAC GAGACATGTC CACTTCCTCA ACTTCTCCAG GTTTGGAGGA 
GACCAGCCTG TCTACATCAA CATCATCAGA GACCCCGTCA GTAGATTTCT GTCTAACTAT 
TTCTTCCGTC GCTTTGGAGA TTGGAGAGGG GAACAGAATC ACATGATCCG CACCCCCAGC 
ATGAGGCAGG AGGAGCGCTA CCTGGACATC AATGAGTGCA TTCTGGAAAA CTACCCTGAG 
TGTTCCAACC CCAGGCTGTT TTACATCATC CCATATTTCT GTGGACAGCA CCCCAGATGT 
AGGGAGCCCG GCGAGTGGGC GCTGGAGCGG GCCAAGCTGA ACGTGAACGA AAACTTCCTG 
CTCGTGGGGA TCCTGGAGGA GCTGGAAGAT GTGCTGCTCT TACTGGAAAG ATTTTTACCT 
CATTACTTCA AGGGTGTGCT GAGCATCTAC AAGGACCCAG AGCACAGGAA ACTTGGCAAC 
ATGACTGTGA CCGTGAGGAA GACTGTCCCC TCCCCCGAGG CGGTGCAGAT TCTCTACCAG 
CGAATGAGAT ACGAGTACGA GTTCTACCAC TACGTCAGAG AACAATTCCA CCTGCTCAAG 
CGCAAGCTTG GACTGAAGTC TCGCGTGAGC GGACCTCCCG TGAGACCACA GTTCTTCATC 
CCCACGCCCC TGGAGACGGA GGAGCCAATC GACGACGAGG AGCAAGATGA CGAAAAATGG 
CTAGAAGATA TTTATAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796361.2
CDS	427..1650
Translation	MKKKQQQHPGGGTDPWPHGAPVGGAPPCLGSCKRRIPLLPFLRFSLRDYGFCMATLLVFCLGSLFYQLSGGPPRFLLDLRQYLGNSTYLDDHGPPPSKVLPFPSQVVYNRVGKCGSRTVVLLLRILSEKHGFNLVTSDIHNKTRLTKNEQMELIKNISTAEQPYLFTRHVHFLNFSRFGGDQPVYINIIRDPVSRFLSNYFFRRFGDWRGEQNHMIRTPSMRQEERYLDINECILENYPECSNPRLFYIIPYFCGQHPRCREPGEWALERAKLNVNENFLLVGILEELEDVLLLLERFLPHYFKGVLSIYKDPEHRKLGNMTVTVRKTVPSPEAVQILYQRMRYEYEFYHYVREQFHLLKRKLGLKSRVSGPPVRPQFFIPTPLETEEPIDDEEQDDEKWLEDIYKR

Target ORF information:

RefSeq Version	NM_177387.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus uronyl-2-sulfotransferase (Ust), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177387.3

ORF Insert Sequence:

ATGAAGAAGA AGCAGCAGCA GCATCCCGGC GGCGGCACGG ATCCCTGGCC CCATGGGGCC 
CCTGTGGGGG GCGCCCCTCC GTGCCTGGGC AGCTGCAAGC GCCGGATCCC GCTGCTGCCT 
TTCCTGCGCT TCTCCCTCCG GGACTACGGT TTCTGCATGG CTACCCTGCT GGTCTTCTGC 
CTGGGCTCCC TCTTCTACCA GCTCAGCGGG GGACCCCCTC GCTTCCTGCT CGACCTGCGG 
CAGTATTTGG GAAATTCCAC TTACTTGGAT GACCATGGAC CACCTCCTAG TAAGGTCCTT 
CCCTTCCCCA GCCAGGTGGT GTACAACAGA GTTGGCAAGT GTGGCAGTCG TACCGTGGTC 
TTGCTTCTGA GAATCTTGTC AGAGAAGCAC GGCTTCAATT TGGTCACATC AGACATTCAC 
AACAAGACCC GGCTTACTAA AAATGAACAA ATGGAACTGA TTAAGAATAT AAGTACCGCC 
GAACAGCCCT ATTTATTCAC GAGACATGTC CACTTCCTCA ACTTCTCCAG GTTTGGAGGA 
GACCAGCCTG TCTACATCAA CATCATCAGA GACCCCGTCA GTAGATTTCT GTCTAACTAT 
TTCTTCCGTC GCTTTGGAGA TTGGAGAGGG GAACAGAATC ACATGATCCG CACCCCCAGC 
ATGAGGCAGG AGGAGCGCTA CCTGGACATC AATGAGTGCA TTCTGGAAAA CTACCCTGAG 
TGTTCCAACC CCAGGCTGTT TTACATCATC CCATATTTCT GTGGACAGCA CCCCAGATGT 
AGGGAGCCCG GCGAGTGGGC GCTGGAGCGG GCCAAGCTGA ACGTGAACGA AAACTTCCTG 
CTCGTGGGGA TCCTGGAGGA GCTGGAAGAT GTGCTGCTCT TACTGGAAAG ATTTTTACCT 
CATTACTTCA AGGGTGTGCT GAGCATCTAC AAGGACCCAG AGCACAGGAA ACTTGGCAAC 
ATGACTGTGA CCGTGAGGAA GACTGTCCCC TCCCCCGAGG CGGTGCAGAT TCTCTACCAG 
CGAATGAGAT ACGAGTACGA GTTCTACCAC TACGTCAGAG AACAATTCCA CCTGCTCAAG 
CGCAAGCTTG GACTGAAGTC TCGCGTGAGC GGACCTCCCG TGAGACCACA GTTCTTCATC 
CCCACGCCCC TGGAGACGGA GGAGCCAATC GACGACGAGG AGCAAGATGA CGAAAAATGG 
CTAGAAGATA TTTATAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ust ( XM_006512772.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512835.1 Mus musculus uronyl-2-sulfotransferase (Ust), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43900
Clone ID Related Accession (Same CDS sequence)	XM_006512772.3
Accession Version	XM_006512772.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	uronyl 2-sulfotransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512772.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG GAGACCAGCC TGTCTACATC AACATCATCA GAGACCCCGT CAGTAGATTT 
CTGTCTAACT ATTTCTTCCG TCGCTTTGGA GATTGGAGAG GGGAACAGAA TCACATGATC 
CGCACCCCCA GCATGAGGCA GGAGGAGCGC TACCTGGACA TCAATGAGTG CATTCTGGAA 
AACTACCCTG AGTGTTCCAA CCCCAGGCTG TTTTACATCA TCCCATATTT CTGTGGACAG 
CACCCCAGAT GTAGGGAGCC CGGCGAGTGG GCGCTGGAGC GGGCCAAGCT GAACGTGAAC 
GAAAACTTCC TGCTCGTGGG GATCCTGGAG GAGCTGGAAG ATGTGCTGCT CTTACTGGAA 
AGATTTTTAC CTCATTACTT CAAGGGTGTG CTGAGCATCT ACAAGGACCC AGAGCACAGG 
AAACTTGGCA ACATGACTGT GACCGTGAGG AAGACTGTCC CCTCCCCCGA GGCGGTGCAG 
ATTCTCTACC AGCGAATGAG ATACGAGTAC GAGTTCTACC ACTACGTCAG AGAACAATTC 
CACCTGCTCA AGCGCAAGCT TGGACTGAAG TCTCGCGTGA GCGGACCTCC CGTGAGACCA 
CAGTTCTTCA TCCCCACGCC CCTGGAGACG GAGGAGCCAA TCGACGACGA GGAGCAAGAT 
GACGAAAAAT GGCTAGAAGA TATTTATAAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512835.1
CDS	1455..2150
Translation	MFGGDQPVYINIIRDPVSRFLSNYFFRRFGDWRGEQNHMIRTPSMRQEERYLDINECILENYPECSNPRLFYIIPYFCGQHPRCREPGEWALERAKLNVNENFLLVGILEELEDVLLLLERFLPHYFKGVLSIYKDPEHRKLGNMTVTVRKTVPSPEAVQILYQRMRYEYEFYHYVREQFHLLKRKLGLKSRVSGPPVRPQFFIPTPLETEEPIDDEEQDDEKWLEDIYKR

Target ORF information:

RefSeq Version	XM_006512772.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus uronyl-2-sulfotransferase (Ust), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512772.3

ORF Insert Sequence:

ATGTTTGGAG GAGACCAGCC TGTCTACATC AACATCATCA GAGACCCCGT CAGTAGATTT 
CTGTCTAACT ATTTCTTCCG TCGCTTTGGA GATTGGAGAG GGGAACAGAA TCACATGATC 
CGCACCCCCA GCATGAGGCA GGAGGAGCGC TACCTGGACA TCAATGAGTG CATTCTGGAA 
AACTACCCTG AGTGTTCCAA CCCCAGGCTG TTTTACATCA TCCCATATTT CTGTGGACAG 
CACCCCAGAT GTAGGGAGCC CGGCGAGTGG GCGCTGGAGC GGGCCAAGCT GAACGTGAAC 
GAAAACTTCC TGCTCGTGGG GATCCTGGAG GAGCTGGAAG ATGTGCTGCT CTTACTGGAA 
AGATTTTTAC CTCATTACTT CAAGGGTGTG CTGAGCATCT ACAAGGACCC AGAGCACAGG 
AAACTTGGCA ACATGACTGT GACCGTGAGG AAGACTGTCC CCTCCCCCGA GGCGGTGCAG 
ATTCTCTACC AGCGAATGAG ATACGAGTAC GAGTTCTACC ACTACGTCAG AGAACAATTC 
CACCTGCTCA AGCGCAAGCT TGGACTGAAG TCTCGCGTGA GCGGACCTCC CGTGAGACCA 
CAGTTCTTCA TCCCCACGCC CCTGGAGACG GAGGAGCCAA TCGACGACGA GGAGCAAGAT 
GACGAAAAAT GGCTAGAAGA TATTTATAAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity.
Cell171(3)522-539.e20(2017 Oct)
Paul A,Crow M,Raudales R,He M,Gillis J,Huang ZJ

Melanoma Cell Adhesion and Migration Is Modulated by the Uronyl 2-O Sulfotransferase.
PloS one12(1)e0170054(2017)
Nikolovska K,Spillmann D,Haier J,Lad?nyi A,Stock C,Seidler DG

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Developmental biology406(1)92-103(2015 Oct)
Lewandowski JP,Du F,Zhang S,Powell MB,Falkenstein KN,Ji H,Vokes SA

Opposing functions of chondroitin sulfate and heparan sulfate during early neuronal polarization.
Neuroscience169(4)1535-47(2010 Sep)
Nishimura K,Ishii M,Kuraoka M,Kamimura K,Maeda N

Oversulfated chondroitin sulfate plays critical roles in the neuronal migration in the cerebral cortex.
The Journal of biological chemistry283(47)32610-20(2008 Nov)
Ishii M,Maeda N