Hecw2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006496066.3	XP_006496129.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_006496067.1	XP_006496130.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_011238537.2	XP_011236839.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_006496066.4	XP_006496129.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_006496069.4	XP_006496132.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_017321236.1	XP_017176725.1	E3 ubiquitin-protein ligase HECW2 isoform X4
NM_001001883.3	NP_001001883.1	E3 ubiquitin-protein ligase HECW2 isoform 1
XM_006496067.2	XP_006496130.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_006496068.4	XP_006496131.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_011238537.3	XP_011236839.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_017321236.2	XP_017176725.1	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_006496069.3	XP_006496132.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_017321235.1	XP_017176724.1	E3 ubiquitin-protein ligase HECW2 isoform X3
NM_172655.3	NP_766243.2	E3 ubiquitin-protein ligase HECW2 isoform 2
XM_006496068.3	XP_006496131.1	E3 ubiquitin-protein ligase HECW2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03833	NM_172655.3 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu27471	XM_011238537.3 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu27471	XM_006496069.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu77170	XM_017321235.1 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu77171	XM_017321236.2 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu21555	XM_006496066.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu21555	XM_006496068.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu21555	XM_006496067.2 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu21555	NM_001001883.3 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu21555	XM_006496066.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu21555	XM_006496067.1	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu27471	XM_011238537.2	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu77171	XM_017321236.1	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu27471	XM_006496069.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu21555	XM_006496068.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

Hecw2 ( NM_172655.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766243.2 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu03833
Clone ID Related Accession (Same CDS sequence)	NM_172655.3
Accession Version	NM_172655.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK042922.1 and AC150896.6. On Oct 31, 2008 this sequence version replaced NM_172655.2. ##Evidence-Data-START## Transcript exon combination :: AK042922.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849382, SAMN01164131 [ECO:0000350] ##Evidence-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766243.2
CDS	95..979
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAG

Target ORF information:

RefSeq Version	NM_172655.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172655.3

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496069.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496132.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu27471
Clone ID Related Accession (Same CDS sequence)	XM_011238537.2 , XM_006496069.4 , XM_011238537.3 , XM_006496069.3
Accession Version	XM_006496069.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496069.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496132.1
CDS	811..5154
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496069.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496069.4

ORF Insert Sequence:

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_011238537.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236839.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X5, mRNA.

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CloneID	OMu27471
Clone ID Related Accession (Same CDS sequence)	XM_011238537.2 , XM_006496069.4 , XM_011238537.3 , XM_006496069.3
Accession Version	XM_011238537.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236839.1
CDS	381..4724
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011238537.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238537.3

ORF Insert Sequence:

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_017321235.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176724.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu77170
Clone ID Related Accession (Same CDS sequence)	XM_017321235.1
Accession Version	XM_017321235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG AAGGCATGGA AGGAGGTGCT TCAGGAAGCC TGCACTCTAG GAAACTCGTC 
AGCTTCACAT TGTCAGATCT CAGGGCAGTT GGGCTCAAGA AAGGGATGTT CTTCAATCCT 
GACCCTTATC TTAAGATGTC CATTCAGCCA GGGAAGAAGA GCAGTTTTCC TACCTGCGCC 
CACCATGGGC AGGAGAGAAG GTCCACTATC ATCAGCAACA CCACCAATCC GATCTGGCAC 
AGAGAGAAAT ACTCCTTTTT TGCACTTTTG ACTGATGTCT TAGAAATTGA AATCAAAGAC 
AAATTTGCCA AGAGCCGGCC CATCATCAAA CGCTTCCTGG GGAAACTAAC CATTCCAGTG 
CAGAGGCTGC TGGAGCGGCA GGCTGGGGAC CAAATGCTCA GCTACAACCT TGGCAGACGG 
CTCCCAGCTG ACCACGTGAG TGGGTACCTC CAGTTTAAAG TGGAAGTTAC ATCTTCTGCT 
CACGAAGATG CTTCTCCAGA GGCTGTCGGC ACCATCCTCG GAGTCCACAC TGTGAATGGA 
GACCTGGGCA GCCCTTCCGA TGAGGAGGAT ATGCCAGGCA GCCATCATGA CAGTACTATC 
TGTGCTAATG GGCCAGTGTC CGAGGACAGT GTTGCTGATG GCACCCCTAA GCATTCTTTC 
AGGACTAGCT CTACCCTGGA GATCGACACG GAGGACTTGA TCTCTACTTC TTCTAGAAAT 
TCACCCCCCA GGGGCCGCCA GGATTCCCTC AATGATTACT TAGATGCTAT TGAACACAAT 
GGCCCCGCCA GGCCAGGGGC AGCCTCCTCC TCTGAGCGGT CCATGGGAGC CTCTCCTAAA 
CTGAGGAGTA GTTTTCCCAC TGACACAAGA CTCAATGCGA TGCTTCATAT TGACTCAGAC 
GAAGAAGACC ATGAGTTTCA GCAAGACTTG GGCTACCCAT CTTCTTTGGA GGAGGAGGGA 
GGCTTGATCA TGTGCAGCAG GGCGTCGAGA ATTGATGATG GGAGCCTGAC ATCTCAGACC 
AAACCAGAGG ATGACAACCC TGTGGAGAAT GAGGATGCCT CTATACATGA AACTGCATCC 
TTAGAAGAGA GGCTGGAGAA TCTTCCAGAG GTTGCAGATG GCTCCTTGCC ATCAAGTACA 
GCCCCTGATG AAAATGAAGC CAACCTGGAG CCTCAACCCA GTGCAGACCA GGGTAGCACT 
GAATTGTGTA GCTCTCAAGA AGTAGATCAG CCCACAAGTG GTGCAGATGC AGGAGCTTCT 
GACACATCTG GAGGGAGCCG CAGAGCTGCC AGTGAGACCG AGTCCCTGGA CCAGGGATCT 
GAACCTTCCC AGGTGTCCTC TGAGACAGAA CCCAGTGACC CTGCAAGGAC AGAGAGTGTG 
AGTGAGGCCA GTACCAGACC TGAGGGGGAG AGTGACCCGG AAGGCGCAGA CAGCTCATGC 
AATGAGAGCG TGACTACACA GCTGTCCTCC GTGGAGACAC GGTGCTCATC TCTAGAGAGT 
GCGCGATTTC CAGAAACCCC CGCCTTTTCT TCTCAGGAGG AAGAAGATGG GGCCTGTGCA 
GCTGAGCCCA CCAGCAGTGG CCCCGCAGAG GGGTCGCAGG AGTCCGTCTG CACTCCTAGT 
TCTTTACCTG CTGTGCAGGT GCCCAGCAGG GAAGAGGAAG GGTCAGCGGC TGAAGCTGCT 
GCTTTATCTG AGCAGGGGGA GCTAGGGGAA GTCTGGCAGA GGAGGGGGAG TCTTGAGGGA 
GCAGCCGCTG CTGCTCCTGC TGCTGCTGCC ACTGATAGCC AGCCCCAGGA AGATGGGGAT 
GCTGGGGATG CCCAAGGTGC CTGCGAAGGG GCCACTGCCC AGGAGGAGGG CGCCACTGGA 
GGTTCTCAAA CCAATGGCCA CCAGCCCTTG AGGTCACTAC CTTCCGTGCG CCAGGACGTC 
AGCCGGTACC AGAGGGTGGA TGAGGCTCTC CCACCAAACT GGGAGGCACG GATTGACAGC 
CACGGCAGGA TCTTCTATGT GGACCATGTA AACAGAACCA CAACGTGGCA GCGGCCCACA 
GCTCCCCCTG CCCCACAGGT CCTGCAGCGA TCTAACTCCA TACAGCAAAT GGAGCAGCTG 
AACCGACGTT ATCAGAGCAT CCGCAGAACC ATGACTAATG AGAGGCCTGA GGAAAATACC 
AGTGCTATCG ATGGGGCAGG GGAGGAAGCC GACTTTCACC AAGCCAGCGC AGACTTCCGT 
CGGGAGAACG TCCTGCCTCA CTCTACCTCC AGATCCAGGC TCACGCTGCT GTTACAGTCA 
CCCCCCGTGA AGTTCCTCAT CAGCCCAGAG TTCTTCACCG TGCTGCATTC TAACCCTAGT 
GCCTACCGCA TGTTTACAAA CAACACGTGT TTGAAGCACA TGATCACCAA AGTCCGGCGG 
GACACCCACC ACTTTGAGCG CTATCAGCAT AACAGGGACC TCGTGGGATT CCTCAACATG 
TTTGCTAACA AACAGCTGGA ACTACCAAGG GGCTGGGAGA TGAAACATGA TCATCAGGGC 
AAGGCGTTTT TTGTCGACCA CAACTCCCGG ACCACCACGT TCATTGATCC TCGGCTCCCA 
CTTCAGAGCA GTAGGCCCAC AAGTGCATTG GTTCATCGAC AACACCTGAC GAGGCAACGC 
AGCCACAGTG CGGGTGAGGT AGGAGAAGAC TCTCGGCACG CAGGACCACC AGTTCTTCCC 
AGGCCATCCA GTACATTCAA TACGGTCAGT AGGCCGCAGT ACCAGGACAT GGTTCCAGTG 
GCCTACAATG ACAAAATTGT TGCGTTTTTG CGTCAACCCA ACATCCTTGA AATTCTGCAA 
GAGCGTCAAC CTGATCTTGC CAGAAACCAC TCACTCAGGG AGAAGATCCA ATTTATCCGA 
ACGGAAGGGA CCCCTGGATT GGTGCGGCTC TCAAGCGATG CAGACCTTGT CATGTTACTG 
AGCTTATTCG AGGAAGAGAT AATGTCATAT GTGCCTCCTC ATGCCTTACT CCACCCCAGC 
TACTGTCAGT CCCCGCGTGG CTCCCCCGTA TCATCTCCCC AGAACTCACC AGGTACTCAG 
CGTGCCAATG CCCGGGCTCC AGCCCCTTAC AAGAGAGATT TTGAAGCCAA ACTAAGGAAC 
TTTTACAGGA AGTTAGAAAC TAAAGGATAC GGACAAGGCC CAGGAAAATT AAAGCTAATT 
ATCCGAAGAG ATCACTTGCT GGAAGATGCT TTCAATCAGA TCATGGGCTA CTCCAGAAAG 
GACCTGCAGA GGAATAAACT GTACGTCACC TTTGTCGGGG AGGAAGGGTT GGATTACAGT 
GGGCCATCCA GAGAGTTCTT CTTCCTGGTA TCCCGAGAGC TCTTTAACCC CTATTACGGC 
TTATTTGAAT ATTCAGCCAA TGACACATAT ACAGTGCAAA TAAGTCCCAT GTCTGCCTTT 
GTAGACAATC ATCATGAATG GTTCCGGTTC AGCGGGAGGA TCCTCGGCCT TGCGCTCATA 
CACCAGTACT TGCTGGACGC CTTCTTCACG CGACCCTTCT ATAAGGCTCT TCTCAGAATC 
CTGTGTGACC TGAGTGATCT GGAATACCTT GATGAGGAGT TCCACCAGAG CCTTCAGTGG 
ATGAAAGACA ATGACATCCA TGACATCCTG GACCTCACGT TCACTGTGAA TGAAGAAGTT 
TTTGGGCAGA TTACTGAACG AGAGTTAAAA CCAGGGGGAG CCAATATTCC AGTCACAGAG 
AAGAACAAGA AGGAGTACAT CGAGAGAATG GTGAAGTGGA GGATCGAGAG GGGTGTGGTG 
CAGCAGACAG AGAGCCTAGT TCGTGGTTTC TACGAGGTAG TAGATGCCAG GCTAGTGTCT 
GTCTTTGATG CGAGAGAGCT GGAACTGGTC ATTGCCGGCA CAGCTGAGAT AGATCTCAAT 
GACTGGAGAA ACAACACTGA ATACAGAGGC GGGTACCATG ACAATCACAT TGTAATTCGG 
TGGTTCTGGG CTGCAGTGGA AAGATTCAAC AATGAACAAC GACTAAGGTT GTTACAGTTT 
GTTACAGGCA CATCCAGCAT TCCCTATGAA GGATTCGCTT CTCTCCGAGG GAGTAATGGT 
CCAAGAAGAT TCTGTGTGGA AAAATGGGGA AAAATCACCG CTCTTCCCAG AGCCCACACT 
TGTTTTAACC GCCTGGATCT GCCTCCATAC CCTTCCTTTT CCATGCTTTA TGAAAAACTC 
TTGACAGCAG TTGAAGAGAC CAGTACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176724.1
CDS	1024..5265
Translation	MGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017321235.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321235.1

ORF Insert Sequence:

ATGGGGGCCG AAGGCATGGA AGGAGGTGCT TCAGGAAGCC TGCACTCTAG GAAACTCGTC 
AGCTTCACAT TGTCAGATCT CAGGGCAGTT GGGCTCAAGA AAGGGATGTT CTTCAATCCT 
GACCCTTATC TTAAGATGTC CATTCAGCCA GGGAAGAAGA GCAGTTTTCC TACCTGCGCC 
CACCATGGGC AGGAGAGAAG GTCCACTATC ATCAGCAACA CCACCAATCC GATCTGGCAC 
AGAGAGAAAT ACTCCTTTTT TGCACTTTTG ACTGATGTCT TAGAAATTGA AATCAAAGAC 
AAATTTGCCA AGAGCCGGCC CATCATCAAA CGCTTCCTGG GGAAACTAAC CATTCCAGTG 
CAGAGGCTGC TGGAGCGGCA GGCTGGGGAC CAAATGCTCA GCTACAACCT TGGCAGACGG 
CTCCCAGCTG ACCACGTGAG TGGGTACCTC CAGTTTAAAG TGGAAGTTAC ATCTTCTGCT 
CACGAAGATG CTTCTCCAGA GGCTGTCGGC ACCATCCTCG GAGTCCACAC TGTGAATGGA 
GACCTGGGCA GCCCTTCCGA TGAGGAGGAT ATGCCAGGCA GCCATCATGA CAGTACTATC 
TGTGCTAATG GGCCAGTGTC CGAGGACAGT GTTGCTGATG GCACCCCTAA GCATTCTTTC 
AGGACTAGCT CTACCCTGGA GATCGACACG GAGGACTTGA TCTCTACTTC TTCTAGAAAT 
TCACCCCCCA GGGGCCGCCA GGATTCCCTC AATGATTACT TAGATGCTAT TGAACACAAT 
GGCCCCGCCA GGCCAGGGGC AGCCTCCTCC TCTGAGCGGT CCATGGGAGC CTCTCCTAAA 
CTGAGGAGTA GTTTTCCCAC TGACACAAGA CTCAATGCGA TGCTTCATAT TGACTCAGAC 
GAAGAAGACC ATGAGTTTCA GCAAGACTTG GGCTACCCAT CTTCTTTGGA GGAGGAGGGA 
GGCTTGATCA TGTGCAGCAG GGCGTCGAGA ATTGATGATG GGAGCCTGAC ATCTCAGACC 
AAACCAGAGG ATGACAACCC TGTGGAGAAT GAGGATGCCT CTATACATGA AACTGCATCC 
TTAGAAGAGA GGCTGGAGAA TCTTCCAGAG GTTGCAGATG GCTCCTTGCC ATCAAGTACA 
GCCCCTGATG AAAATGAAGC CAACCTGGAG CCTCAACCCA GTGCAGACCA GGGTAGCACT 
GAATTGTGTA GCTCTCAAGA AGTAGATCAG CCCACAAGTG GTGCAGATGC AGGAGCTTCT 
GACACATCTG GAGGGAGCCG CAGAGCTGCC AGTGAGACCG AGTCCCTGGA CCAGGGATCT 
GAACCTTCCC AGGTGTCCTC TGAGACAGAA CCCAGTGACC CTGCAAGGAC AGAGAGTGTG 
AGTGAGGCCA GTACCAGACC TGAGGGGGAG AGTGACCCGG AAGGCGCAGA CAGCTCATGC 
AATGAGAGCG TGACTACACA GCTGTCCTCC GTGGAGACAC GGTGCTCATC TCTAGAGAGT 
GCGCGATTTC CAGAAACCCC CGCCTTTTCT TCTCAGGAGG AAGAAGATGG GGCCTGTGCA 
GCTGAGCCCA CCAGCAGTGG CCCCGCAGAG GGGTCGCAGG AGTCCGTCTG CACTCCTAGT 
TCTTTACCTG CTGTGCAGGT GCCCAGCAGG GAAGAGGAAG GGTCAGCGGC TGAAGCTGCT 
GCTTTATCTG AGCAGGGGGA GCTAGGGGAA GTCTGGCAGA GGAGGGGGAG TCTTGAGGGA 
GCAGCCGCTG CTGCTCCTGC TGCTGCTGCC ACTGATAGCC AGCCCCAGGA AGATGGGGAT 
GCTGGGGATG CCCAAGGTGC CTGCGAAGGG GCCACTGCCC AGGAGGAGGG CGCCACTGGA 
GGTTCTCAAA CCAATGGCCA CCAGCCCTTG AGGTCACTAC CTTCCGTGCG CCAGGACGTC 
AGCCGGTACC AGAGGGTGGA TGAGGCTCTC CCACCAAACT GGGAGGCACG GATTGACAGC 
CACGGCAGGA TCTTCTATGT GGACCATGTA AACAGAACCA CAACGTGGCA GCGGCCCACA 
GCTCCCCCTG CCCCACAGGT CCTGCAGCGA TCTAACTCCA TACAGCAAAT GGAGCAGCTG 
AACCGACGTT ATCAGAGCAT CCGCAGAACC ATGACTAATG AGAGGCCTGA GGAAAATACC 
AGTGCTATCG ATGGGGCAGG GGAGGAAGCC GACTTTCACC AAGCCAGCGC AGACTTCCGT 
CGGGAGAACG TCCTGCCTCA CTCTACCTCC AGATCCAGGC TCACGCTGCT GTTACAGTCA 
CCCCCCGTGA AGTTCCTCAT CAGCCCAGAG TTCTTCACCG TGCTGCATTC TAACCCTAGT 
GCCTACCGCA TGTTTACAAA CAACACGTGT TTGAAGCACA TGATCACCAA AGTCCGGCGG 
GACACCCACC ACTTTGAGCG CTATCAGCAT AACAGGGACC TCGTGGGATT CCTCAACATG 
TTTGCTAACA AACAGCTGGA ACTACCAAGG GGCTGGGAGA TGAAACATGA TCATCAGGGC 
AAGGCGTTTT TTGTCGACCA CAACTCCCGG ACCACCACGT TCATTGATCC TCGGCTCCCA 
CTTCAGAGCA GTAGGCCCAC AAGTGCATTG GTTCATCGAC AACACCTGAC GAGGCAACGC 
AGCCACAGTG CGGGTGAGGT AGGAGAAGAC TCTCGGCACG CAGGACCACC AGTTCTTCCC 
AGGCCATCCA GTACATTCAA TACGGTCAGT AGGCCGCAGT ACCAGGACAT GGTTCCAGTG 
GCCTACAATG ACAAAATTGT TGCGTTTTTG CGTCAACCCA ACATCCTTGA AATTCTGCAA 
GAGCGTCAAC CTGATCTTGC CAGAAACCAC TCACTCAGGG AGAAGATCCA ATTTATCCGA 
ACGGAAGGGA CCCCTGGATT GGTGCGGCTC TCAAGCGATG CAGACCTTGT CATGTTACTG 
AGCTTATTCG AGGAAGAGAT AATGTCATAT GTGCCTCCTC ATGCCTTACT CCACCCCAGC 
TACTGTCAGT CCCCGCGTGG CTCCCCCGTA TCATCTCCCC AGAACTCACC AGGTACTCAG 
CGTGCCAATG CCCGGGCTCC AGCCCCTTAC AAGAGAGATT TTGAAGCCAA ACTAAGGAAC 
TTTTACAGGA AGTTAGAAAC TAAAGGATAC GGACAAGGCC CAGGAAAATT AAAGCTAATT 
ATCCGAAGAG ATCACTTGCT GGAAGATGCT TTCAATCAGA TCATGGGCTA CTCCAGAAAG 
GACCTGCAGA GGAATAAACT GTACGTCACC TTTGTCGGGG AGGAAGGGTT GGATTACAGT 
GGGCCATCCA GAGAGTTCTT CTTCCTGGTA TCCCGAGAGC TCTTTAACCC CTATTACGGC 
TTATTTGAAT ATTCAGCCAA TGACACATAT ACAGTGCAAA TAAGTCCCAT GTCTGCCTTT 
GTAGACAATC ATCATGAATG GTTCCGGTTC AGCGGGAGGA TCCTCGGCCT TGCGCTCATA 
CACCAGTACT TGCTGGACGC CTTCTTCACG CGACCCTTCT ATAAGGCTCT TCTCAGAATC 
CTGTGTGACC TGAGTGATCT GGAATACCTT GATGAGGAGT TCCACCAGAG CCTTCAGTGG 
ATGAAAGACA ATGACATCCA TGACATCCTG GACCTCACGT TCACTGTGAA TGAAGAAGTT 
TTTGGGCAGA TTACTGAACG AGAGTTAAAA CCAGGGGGAG CCAATATTCC AGTCACAGAG 
AAGAACAAGA AGGAGTACAT CGAGAGAATG GTGAAGTGGA GGATCGAGAG GGGTGTGGTG 
CAGCAGACAG AGAGCCTAGT TCGTGGTTTC TACGAGGTAG TAGATGCCAG GCTAGTGTCT 
GTCTTTGATG CGAGAGAGCT GGAACTGGTC ATTGCCGGCA CAGCTGAGAT AGATCTCAAT 
GACTGGAGAA ACAACACTGA ATACAGAGGC GGGTACCATG ACAATCACAT TGTAATTCGG 
TGGTTCTGGG CTGCAGTGGA AAGATTCAAC AATGAACAAC GACTAAGGTT GTTACAGTTT 
GTTACAGGCA CATCCAGCAT TCCCTATGAA GGATTCGCTT CTCTCCGAGG GAGTAATGGT 
CCAAGAAGAT TCTGTGTGGA AAAATGGGGA AAAATCACCG CTCTTCCCAG AGCCCACACT 
TGTTTTAACC GCCTGGATCT GCCTCCATAC CCTTCCTTTT CCATGCTTTA TGAAAAACTC 
TTGACAGCAG TTGAAGAGAC CAGTACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_017321236.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176725.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X7, mRNA.

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CloneID	OMu77171
Clone ID Related Accession (Same CDS sequence)	XM_017321236.1 , XM_017321236.2
Accession Version	XM_017321236.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC TCTAGGAAAC 
TCGTCAGCTT CACATTGTCA GCCAGGGAAG AAGAGCAGTT TTCCTACCTG CGCCCACCAT 
GGGCAGGAGA GAAGGTCCAC TATCATCAGC AACACCACCA ATCCGATCTG GCACAGAGAG 
AAATACTCCT TTTTTGCACT TTTGACTGAT GTCTTAGAAA TTGAAATCAA AGACAAATTT 
GCCAAGAGCC GGCCCATCAT CAAACGCTTC CTGGGGAAAC TAACCATTCC AGTGCAGAGG 
CTGCTGGAGC GGCAGGCTGG GGACCAAATG CTCAGCTACA ACCTTGGCAG ACGGCTCCCA 
GCTGACCACG TGAGTGGGTA CCTCCAGTTT AAAGTGGAAG TTACATCTTC TGCTCACGAA 
GATGCTTCTC CAGAGGCTGT CGGCACCATC CTCGGAGTCC ACACTGTGAA TGGAGACCTG 
GGCAGCCCTT CCGATGAGGA GGATATGCCA GGCAGCCATC ATGACAGTAC TATCTGTGCT 
AATGGGCCAG TGTCCGAGGA CAGTGTTGCT GATGGCACCC CTAAGCATTC TTTCAGGACT 
AGCTCTACCC TGGAGATCGA CACGGAGGAC TTGATCTCTA CTTCTTCTAG AAATTCACCC 
CCCAGGGGCC GCCAGGATTC CCTCAATGAT TACTTAGATG CTATTGAACA CAATGGCCCC 
GCCAGGCCAG GGGCAGCCTC CTCCTCTGAG CGGTCCATGG GAGCCTCTCC TAAACTGAGG 
AGTAGTTTTC CCACTGACAC AAGACTCAAT GCGATGCTTC ATATTGACTC AGACGAAGAA 
GACCATGAGT TTCAGCAAGA CTTGGGCTAC CCATCTTCTT TGGAGGAGGA GGGAGGCTTG 
ATCATGTGCA GCAGGGCGTC GAGAATTGAT GATGGGAGCC TGACATCTCA GACCAAACCA 
GAGGATGACA ACCCTGTGGA GAATGAGGAT GCCTCTATAC ATGAAACTGC ATCCTTAGAA 
GAGAGGCTGG AGAATCTTCC AGAGGTTGCA GATGGCTCCT TGCCATCAAG TACAGCCCCT 
GATGAAAATG AAGCCAACCT GGAGCCTCAA CCCAGTGCAG ACCAGGGTAG CACTGAATTG 
TGTAGCTCTC AAGAAGTAGA TCAGCCCACA AGTGGTGCAG ATGCAGGAGC TTCTGACACA 
TCTGGAGGGA GCCGCAGAGC TGCCAGTGAG ACCGAGTCCC TGGACCAGGG ATCTGAACCT 
TCCCAGGTGT CCTCTGAGAC AGAACCCAGT GACCCTGCAA GGACAGAGAG TGTGAGTGAG 
GCCAGTACCA GACCTGAGGG GGAGAGTGAC CCGGAAGGCG CAGACAGCTC ATGCAATGAG 
AGCGTGACTA CACAGCTGTC CTCCGTGGAG ACACGGTGCT CATCTCTAGA GAGTGCGCGA 
TTTCCAGAAA CCCCCGCCTT TTCTTCTCAG GAGGAAGAAG ATGGGGCCTG TGCAGCTGAG 
CCCACCAGCA GTGGCCCCGC AGAGGGGTCG CAGGAGTCCG TCTGCACTCC TAGTTCTTTA 
CCTGCTGTGC AGGTGCCCAG CAGGGAAGAG GAAGGGTCAG CGGCTGAAGC TGCTGCTTTA 
TCTGAGCAGG GGGAGCTAGG GGAAGTCTGG CAGAGGAGGG GGAGTCTTGA GGGAGCAGCC 
GCTGCTGCTC CTGCTGCTGC TGCCACTGAT AGCCAGCCCC AGGAAGATGG GGATGCTGGG 
GATGCCCAAG GTGCCTGCGA AGGGGCCACT GCCCAGGAGG AGGGCGCCAC TGGAGGTTCT 
CAAACCAATG GCCACCAGCC CTTGAGGTCA CTACCTTCCG TGCGCCAGGA CGTCAGCCGG 
TACCAGAGGG TGGATGAGGC TCTCCCACCA AACTGGGAGG CACGGATTGA CAGCCACGGC 
AGGATCTTCT ATGTGGACCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCAC AGGTCCTGCA GCGATCTAAC TCCATACAGC AAATGGAGCA GCTGAACCGA 
CGTTATCAGA GCATCCGCAG AACCATGACT AATGAGAGGC CTGAGGAAAA TACCAGTGCT 
ATCGATGGGG CAGGGGAGGA AGCCGACTTT CACCAAGCCA GCGCAGACTT CCGTCGGGAG 
AACGTCCTGC CTCACTCTAC CTCCAGATCC AGGCTCACGC TGCTGTTACA GTCACCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AGCGCTATCA GCATAACAGG GACCTCGTGG GATTCCTCAA CATGTTTGCT 
AACAAACAGC TGGAACTACC AAGGGGCTGG GAGATGAAAC ATGATCATCA GGGCAAGGCG 
TTTTTTGTCG ACCACAACTC CCGGACCACC ACGTTCATTG ATCCTCGGCT CCCACTTCAG 
AGCAGTAGGC CCACAAGTGC ATTGGTTCAT CGACAACACC TGACGAGGCA ACGCAGCCAC 
AGTGCGGGTG AGGTAGGAGA AGACTCTCGG CACGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCCAGTACAT TCAATACGGT CAGTAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCCTAC 
AATGACAAAA TTGTTGCGTT TTTGCGTCAA CCCAACATCC TTGAAATTCT GCAAGAGCGT 
CAACCTGATC TTGCCAGAAA CCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACGGAA 
GGGACCCCTG GATTGGTGCG GCTCTCAAGC GATGCAGACC TTGTCATGTT ACTGAGCTTA 
TTCGAGGAAG AGATAATGTC ATATGTGCCT CCTCATGCCT TACTCCACCC CAGCTACTGT 
CAGTCCCCGC GTGGCTCCCC CGTATCATCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGGG CTCCAGCCCC TTACAAGAGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AAACTAAAGG ATACGGACAA GGCCCAGGAA AATTAAAGCT AATTATCCGA 
AGAGATCACT TGCTGGAAGA TGCTTTCAAT CAGATCATGG GCTACTCCAG AAAGGACCTG 
CAGAGGAATA AACTGTACGT CACCTTTGTC GGGGAGGAAG GGTTGGATTA CAGTGGGCCA 
TCCAGAGAGT TCTTCTTCCT GGTATCCCGA GAGCTCTTTA ACCCCTATTA CGGCTTATTT 
GAATATTCAG CCAATGACAC ATATACAGTG CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCATCATG AATGGTTCCG GTTCAGCGGG AGGATCCTCG GCCTTGCGCT CATACACCAG 
TACTTGCTGG ACGCCTTCTT CACGCGACCC TTCTATAAGG CTCTTCTCAG AATCCTGTGT 
GACCTGAGTG ATCTGGAATA CCTTGATGAG GAGTTCCACC AGAGCCTTCA GTGGATGAAA 
GACAATGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAGTT AAAACCAGGG GGAGCCAATA TTCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG AATGGTGAAG TGGAGGATCG AGAGGGGTGT GGTGCAGCAG 
ACAGAGAGCC TAGTTCGTGG TTTCTACGAG GTAGTAGATG CCAGGCTAGT GTCTGTCTTT 
GATGCGAGAG AGCTGGAACT GGTCATTGCC GGCACAGCTG AGATAGATCT CAATGACTGG 
AGAAACAACA CTGAATACAG AGGCGGGTAC CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTAA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTC GCTTCTCTCC GAGGGAGTAA TGGTCCAAGA 
AGATTCTGTG TGGAAAAATG GGGAAAAATC ACCGCTCTTC CCAGAGCCCA CACTTGTTTT 
AACCGCCTGG ATCTGCCTCC ATACCCTTCC TTTTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176725.1
CDS	1019..5194
Translation	MEWGPKAWKEVLQEACTLGNSSASHCQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017321236.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321236.1

ORF Insert Sequence:

ATGGAATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC TCTAGGAAAC 
TCGTCAGCTT CACATTGTCA GCCAGGGAAG AAGAGCAGTT TTCCTACCTG CGCCCACCAT 
GGGCAGGAGA GAAGGTCCAC TATCATCAGC AACACCACCA ATCCGATCTG GCACAGAGAG 
AAATACTCCT TTTTTGCACT TTTGACTGAT GTCTTAGAAA TTGAAATCAA AGACAAATTT 
GCCAAGAGCC GGCCCATCAT CAAACGCTTC CTGGGGAAAC TAACCATTCC AGTGCAGAGG 
CTGCTGGAGC GGCAGGCTGG GGACCAAATG CTCAGCTACA ACCTTGGCAG ACGGCTCCCA 
GCTGACCACG TGAGTGGGTA CCTCCAGTTT AAAGTGGAAG TTACATCTTC TGCTCACGAA 
GATGCTTCTC CAGAGGCTGT CGGCACCATC CTCGGAGTCC ACACTGTGAA TGGAGACCTG 
GGCAGCCCTT CCGATGAGGA GGATATGCCA GGCAGCCATC ATGACAGTAC TATCTGTGCT 
AATGGGCCAG TGTCCGAGGA CAGTGTTGCT GATGGCACCC CTAAGCATTC TTTCAGGACT 
AGCTCTACCC TGGAGATCGA CACGGAGGAC TTGATCTCTA CTTCTTCTAG AAATTCACCC 
CCCAGGGGCC GCCAGGATTC CCTCAATGAT TACTTAGATG CTATTGAACA CAATGGCCCC 
GCCAGGCCAG GGGCAGCCTC CTCCTCTGAG CGGTCCATGG GAGCCTCTCC TAAACTGAGG 
AGTAGTTTTC CCACTGACAC AAGACTCAAT GCGATGCTTC ATATTGACTC AGACGAAGAA 
GACCATGAGT TTCAGCAAGA CTTGGGCTAC CCATCTTCTT TGGAGGAGGA GGGAGGCTTG 
ATCATGTGCA GCAGGGCGTC GAGAATTGAT GATGGGAGCC TGACATCTCA GACCAAACCA 
GAGGATGACA ACCCTGTGGA GAATGAGGAT GCCTCTATAC ATGAAACTGC ATCCTTAGAA 
GAGAGGCTGG AGAATCTTCC AGAGGTTGCA GATGGCTCCT TGCCATCAAG TACAGCCCCT 
GATGAAAATG AAGCCAACCT GGAGCCTCAA CCCAGTGCAG ACCAGGGTAG CACTGAATTG 
TGTAGCTCTC AAGAAGTAGA TCAGCCCACA AGTGGTGCAG ATGCAGGAGC TTCTGACACA 
TCTGGAGGGA GCCGCAGAGC TGCCAGTGAG ACCGAGTCCC TGGACCAGGG ATCTGAACCT 
TCCCAGGTGT CCTCTGAGAC AGAACCCAGT GACCCTGCAA GGACAGAGAG TGTGAGTGAG 
GCCAGTACCA GACCTGAGGG GGAGAGTGAC CCGGAAGGCG CAGACAGCTC ATGCAATGAG 
AGCGTGACTA CACAGCTGTC CTCCGTGGAG ACACGGTGCT CATCTCTAGA GAGTGCGCGA 
TTTCCAGAAA CCCCCGCCTT TTCTTCTCAG GAGGAAGAAG ATGGGGCCTG TGCAGCTGAG 
CCCACCAGCA GTGGCCCCGC AGAGGGGTCG CAGGAGTCCG TCTGCACTCC TAGTTCTTTA 
CCTGCTGTGC AGGTGCCCAG CAGGGAAGAG GAAGGGTCAG CGGCTGAAGC TGCTGCTTTA 
TCTGAGCAGG GGGAGCTAGG GGAAGTCTGG CAGAGGAGGG GGAGTCTTGA GGGAGCAGCC 
GCTGCTGCTC CTGCTGCTGC TGCCACTGAT AGCCAGCCCC AGGAAGATGG GGATGCTGGG 
GATGCCCAAG GTGCCTGCGA AGGGGCCACT GCCCAGGAGG AGGGCGCCAC TGGAGGTTCT 
CAAACCAATG GCCACCAGCC CTTGAGGTCA CTACCTTCCG TGCGCCAGGA CGTCAGCCGG 
TACCAGAGGG TGGATGAGGC TCTCCCACCA AACTGGGAGG CACGGATTGA CAGCCACGGC 
AGGATCTTCT ATGTGGACCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCAC AGGTCCTGCA GCGATCTAAC TCCATACAGC AAATGGAGCA GCTGAACCGA 
CGTTATCAGA GCATCCGCAG AACCATGACT AATGAGAGGC CTGAGGAAAA TACCAGTGCT 
ATCGATGGGG CAGGGGAGGA AGCCGACTTT CACCAAGCCA GCGCAGACTT CCGTCGGGAG 
AACGTCCTGC CTCACTCTAC CTCCAGATCC AGGCTCACGC TGCTGTTACA GTCACCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AGCGCTATCA GCATAACAGG GACCTCGTGG GATTCCTCAA CATGTTTGCT 
AACAAACAGC TGGAACTACC AAGGGGCTGG GAGATGAAAC ATGATCATCA GGGCAAGGCG 
TTTTTTGTCG ACCACAACTC CCGGACCACC ACGTTCATTG ATCCTCGGCT CCCACTTCAG 
AGCAGTAGGC CCACAAGTGC ATTGGTTCAT CGACAACACC TGACGAGGCA ACGCAGCCAC 
AGTGCGGGTG AGGTAGGAGA AGACTCTCGG CACGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCCAGTACAT TCAATACGGT CAGTAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCCTAC 
AATGACAAAA TTGTTGCGTT TTTGCGTCAA CCCAACATCC TTGAAATTCT GCAAGAGCGT 
CAACCTGATC TTGCCAGAAA CCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACGGAA 
GGGACCCCTG GATTGGTGCG GCTCTCAAGC GATGCAGACC TTGTCATGTT ACTGAGCTTA 
TTCGAGGAAG AGATAATGTC ATATGTGCCT CCTCATGCCT TACTCCACCC CAGCTACTGT 
CAGTCCCCGC GTGGCTCCCC CGTATCATCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGGG CTCCAGCCCC TTACAAGAGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AAACTAAAGG ATACGGACAA GGCCCAGGAA AATTAAAGCT AATTATCCGA 
AGAGATCACT TGCTGGAAGA TGCTTTCAAT CAGATCATGG GCTACTCCAG AAAGGACCTG 
CAGAGGAATA AACTGTACGT CACCTTTGTC GGGGAGGAAG GGTTGGATTA CAGTGGGCCA 
TCCAGAGAGT TCTTCTTCCT GGTATCCCGA GAGCTCTTTA ACCCCTATTA CGGCTTATTT 
GAATATTCAG CCAATGACAC ATATACAGTG CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCATCATG AATGGTTCCG GTTCAGCGGG AGGATCCTCG GCCTTGCGCT CATACACCAG 
TACTTGCTGG ACGCCTTCTT CACGCGACCC TTCTATAAGG CTCTTCTCAG AATCCTGTGT 
GACCTGAGTG ATCTGGAATA CCTTGATGAG GAGTTCCACC AGAGCCTTCA GTGGATGAAA 
GACAATGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAGTT AAAACCAGGG GGAGCCAATA TTCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG AATGGTGAAG TGGAGGATCG AGAGGGGTGT GGTGCAGCAG 
ACAGAGAGCC TAGTTCGTGG TTTCTACGAG GTAGTAGATG CCAGGCTAGT GTCTGTCTTT 
GATGCGAGAG AGCTGGAACT GGTCATTGCC GGCACAGCTG AGATAGATCT CAATGACTGG 
AGAAACAACA CTGAATACAG AGGCGGGTAC CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTAA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTC GCTTCTCTCC GAGGGAGTAA TGGTCCAAGA 
AGATTCTGTG TGGAAAAATG GGGAAAAATC ACCGCTCTTC CCAGAGCCCA CACTTGTTTT 
AACCGCCTGG ATCTGCCTCC ATACCCTTCC TTTTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_017321236.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176725.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X6, mRNA.

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CloneID	OMu77171
Clone ID Related Accession (Same CDS sequence)	XM_017321236.1 , XM_017321236.2
Accession Version	XM_017321236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC TCTAGGAAAC 
TCGTCAGCTT CACATTGTCA GCCAGGGAAG AAGAGCAGTT TTCCTACCTG CGCCCACCAT 
GGGCAGGAGA GAAGGTCCAC TATCATCAGC AACACCACCA ATCCGATCTG GCACAGAGAG 
AAATACTCCT TTTTTGCACT TTTGACTGAT GTCTTAGAAA TTGAAATCAA AGACAAATTT 
GCCAAGAGCC GGCCCATCAT CAAACGCTTC CTGGGGAAAC TAACCATTCC AGTGCAGAGG 
CTGCTGGAGC GGCAGGCTGG GGACCAAATG CTCAGCTACA ACCTTGGCAG ACGGCTCCCA 
GCTGACCACG TGAGTGGGTA CCTCCAGTTT AAAGTGGAAG TTACATCTTC TGCTCACGAA 
GATGCTTCTC CAGAGGCTGT CGGCACCATC CTCGGAGTCC ACACTGTGAA TGGAGACCTG 
GGCAGCCCTT CCGATGAGGA GGATATGCCA GGCAGCCATC ATGACAGTAC TATCTGTGCT 
AATGGGCCAG TGTCCGAGGA CAGTGTTGCT GATGGCACCC CTAAGCATTC TTTCAGGACT 
AGCTCTACCC TGGAGATCGA CACGGAGGAC TTGATCTCTA CTTCTTCTAG AAATTCACCC 
CCCAGGGGCC GCCAGGATTC CCTCAATGAT TACTTAGATG CTATTGAACA CAATGGCCCC 
GCCAGGCCAG GGGCAGCCTC CTCCTCTGAG CGGTCCATGG GAGCCTCTCC TAAACTGAGG 
AGTAGTTTTC CCACTGACAC AAGACTCAAT GCGATGCTTC ATATTGACTC AGACGAAGAA 
GACCATGAGT TTCAGCAAGA CTTGGGCTAC CCATCTTCTT TGGAGGAGGA GGGAGGCTTG 
ATCATGTGCA GCAGGGCGTC GAGAATTGAT GATGGGAGCC TGACATCTCA GACCAAACCA 
GAGGATGACA ACCCTGTGGA GAATGAGGAT GCCTCTATAC ATGAAACTGC ATCCTTAGAA 
GAGAGGCTGG AGAATCTTCC AGAGGTTGCA GATGGCTCCT TGCCATCAAG TACAGCCCCT 
GATGAAAATG AAGCCAACCT GGAGCCTCAA CCCAGTGCAG ACCAGGGTAG CACTGAATTG 
TGTAGCTCTC AAGAAGTAGA TCAGCCCACA AGTGGTGCAG ATGCAGGAGC TTCTGACACA 
TCTGGAGGGA GCCGCAGAGC TGCCAGTGAG ACCGAGTCCC TGGACCAGGG ATCTGAACCT 
TCCCAGGTGT CCTCTGAGAC AGAACCCAGT GACCCTGCAA GGACAGAGAG TGTGAGTGAG 
GCCAGTACCA GACCTGAGGG GGAGAGTGAC CCGGAAGGCG CAGACAGCTC ATGCAATGAG 
AGCGTGACTA CACAGCTGTC CTCCGTGGAG ACACGGTGCT CATCTCTAGA GAGTGCGCGA 
TTTCCAGAAA CCCCCGCCTT TTCTTCTCAG GAGGAAGAAG ATGGGGCCTG TGCAGCTGAG 
CCCACCAGCA GTGGCCCCGC AGAGGGGTCG CAGGAGTCCG TCTGCACTCC TAGTTCTTTA 
CCTGCTGTGC AGGTGCCCAG CAGGGAAGAG GAAGGGTCAG CGGCTGAAGC TGCTGCTTTA 
TCTGAGCAGG GGGAGCTAGG GGAAGTCTGG CAGAGGAGGG GGAGTCTTGA GGGAGCAGCC 
GCTGCTGCTC CTGCTGCTGC TGCCACTGAT AGCCAGCCCC AGGAAGATGG GGATGCTGGG 
GATGCCCAAG GTGCCTGCGA AGGGGCCACT GCCCAGGAGG AGGGCGCCAC TGGAGGTTCT 
CAAACCAATG GCCACCAGCC CTTGAGGTCA CTACCTTCCG TGCGCCAGGA CGTCAGCCGG 
TACCAGAGGG TGGATGAGGC TCTCCCACCA AACTGGGAGG CACGGATTGA CAGCCACGGC 
AGGATCTTCT ATGTGGACCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCAC AGGTCCTGCA GCGATCTAAC TCCATACAGC AAATGGAGCA GCTGAACCGA 
CGTTATCAGA GCATCCGCAG AACCATGACT AATGAGAGGC CTGAGGAAAA TACCAGTGCT 
ATCGATGGGG CAGGGGAGGA AGCCGACTTT CACCAAGCCA GCGCAGACTT CCGTCGGGAG 
AACGTCCTGC CTCACTCTAC CTCCAGATCC AGGCTCACGC TGCTGTTACA GTCACCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AGCGCTATCA GCATAACAGG GACCTCGTGG GATTCCTCAA CATGTTTGCT 
AACAAACAGC TGGAACTACC AAGGGGCTGG GAGATGAAAC ATGATCATCA GGGCAAGGCG 
TTTTTTGTCG ACCACAACTC CCGGACCACC ACGTTCATTG ATCCTCGGCT CCCACTTCAG 
AGCAGTAGGC CCACAAGTGC ATTGGTTCAT CGACAACACC TGACGAGGCA ACGCAGCCAC 
AGTGCGGGTG AGGTAGGAGA AGACTCTCGG CACGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCCAGTACAT TCAATACGGT CAGTAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCCTAC 
AATGACAAAA TTGTTGCGTT TTTGCGTCAA CCCAACATCC TTGAAATTCT GCAAGAGCGT 
CAACCTGATC TTGCCAGAAA CCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACGGAA 
GGGACCCCTG GATTGGTGCG GCTCTCAAGC GATGCAGACC TTGTCATGTT ACTGAGCTTA 
TTCGAGGAAG AGATAATGTC ATATGTGCCT CCTCATGCCT TACTCCACCC CAGCTACTGT 
CAGTCCCCGC GTGGCTCCCC CGTATCATCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGGG CTCCAGCCCC TTACAAGAGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AAACTAAAGG ATACGGACAA GGCCCAGGAA AATTAAAGCT AATTATCCGA 
AGAGATCACT TGCTGGAAGA TGCTTTCAAT CAGATCATGG GCTACTCCAG AAAGGACCTG 
CAGAGGAATA AACTGTACGT CACCTTTGTC GGGGAGGAAG GGTTGGATTA CAGTGGGCCA 
TCCAGAGAGT TCTTCTTCCT GGTATCCCGA GAGCTCTTTA ACCCCTATTA CGGCTTATTT 
GAATATTCAG CCAATGACAC ATATACAGTG CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCATCATG AATGGTTCCG GTTCAGCGGG AGGATCCTCG GCCTTGCGCT CATACACCAG 
TACTTGCTGG ACGCCTTCTT CACGCGACCC TTCTATAAGG CTCTTCTCAG AATCCTGTGT 
GACCTGAGTG ATCTGGAATA CCTTGATGAG GAGTTCCACC AGAGCCTTCA GTGGATGAAA 
GACAATGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAGTT AAAACCAGGG GGAGCCAATA TTCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG AATGGTGAAG TGGAGGATCG AGAGGGGTGT GGTGCAGCAG 
ACAGAGAGCC TAGTTCGTGG TTTCTACGAG GTAGTAGATG CCAGGCTAGT GTCTGTCTTT 
GATGCGAGAG AGCTGGAACT GGTCATTGCC GGCACAGCTG AGATAGATCT CAATGACTGG 
AGAAACAACA CTGAATACAG AGGCGGGTAC CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTAA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTC GCTTCTCTCC GAGGGAGTAA TGGTCCAAGA 
AGATTCTGTG TGGAAAAATG GGGAAAAATC ACCGCTCTTC CCAGAGCCCA CACTTGTTTT 
AACCGCCTGG ATCTGCCTCC ATACCCTTCC TTTTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176725.1
CDS	1016..5191
Translation	MEWGPKAWKEVLQEACTLGNSSASHCQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017321236.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321236.2

ORF Insert Sequence:

ATGGAATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC TCTAGGAAAC 
TCGTCAGCTT CACATTGTCA GCCAGGGAAG AAGAGCAGTT TTCCTACCTG CGCCCACCAT 
GGGCAGGAGA GAAGGTCCAC TATCATCAGC AACACCACCA ATCCGATCTG GCACAGAGAG 
AAATACTCCT TTTTTGCACT TTTGACTGAT GTCTTAGAAA TTGAAATCAA AGACAAATTT 
GCCAAGAGCC GGCCCATCAT CAAACGCTTC CTGGGGAAAC TAACCATTCC AGTGCAGAGG 
CTGCTGGAGC GGCAGGCTGG GGACCAAATG CTCAGCTACA ACCTTGGCAG ACGGCTCCCA 
GCTGACCACG TGAGTGGGTA CCTCCAGTTT AAAGTGGAAG TTACATCTTC TGCTCACGAA 
GATGCTTCTC CAGAGGCTGT CGGCACCATC CTCGGAGTCC ACACTGTGAA TGGAGACCTG 
GGCAGCCCTT CCGATGAGGA GGATATGCCA GGCAGCCATC ATGACAGTAC TATCTGTGCT 
AATGGGCCAG TGTCCGAGGA CAGTGTTGCT GATGGCACCC CTAAGCATTC TTTCAGGACT 
AGCTCTACCC TGGAGATCGA CACGGAGGAC TTGATCTCTA CTTCTTCTAG AAATTCACCC 
CCCAGGGGCC GCCAGGATTC CCTCAATGAT TACTTAGATG CTATTGAACA CAATGGCCCC 
GCCAGGCCAG GGGCAGCCTC CTCCTCTGAG CGGTCCATGG GAGCCTCTCC TAAACTGAGG 
AGTAGTTTTC CCACTGACAC AAGACTCAAT GCGATGCTTC ATATTGACTC AGACGAAGAA 
GACCATGAGT TTCAGCAAGA CTTGGGCTAC CCATCTTCTT TGGAGGAGGA GGGAGGCTTG 
ATCATGTGCA GCAGGGCGTC GAGAATTGAT GATGGGAGCC TGACATCTCA GACCAAACCA 
GAGGATGACA ACCCTGTGGA GAATGAGGAT GCCTCTATAC ATGAAACTGC ATCCTTAGAA 
GAGAGGCTGG AGAATCTTCC AGAGGTTGCA GATGGCTCCT TGCCATCAAG TACAGCCCCT 
GATGAAAATG AAGCCAACCT GGAGCCTCAA CCCAGTGCAG ACCAGGGTAG CACTGAATTG 
TGTAGCTCTC AAGAAGTAGA TCAGCCCACA AGTGGTGCAG ATGCAGGAGC TTCTGACACA 
TCTGGAGGGA GCCGCAGAGC TGCCAGTGAG ACCGAGTCCC TGGACCAGGG ATCTGAACCT 
TCCCAGGTGT CCTCTGAGAC AGAACCCAGT GACCCTGCAA GGACAGAGAG TGTGAGTGAG 
GCCAGTACCA GACCTGAGGG GGAGAGTGAC CCGGAAGGCG CAGACAGCTC ATGCAATGAG 
AGCGTGACTA CACAGCTGTC CTCCGTGGAG ACACGGTGCT CATCTCTAGA GAGTGCGCGA 
TTTCCAGAAA CCCCCGCCTT TTCTTCTCAG GAGGAAGAAG ATGGGGCCTG TGCAGCTGAG 
CCCACCAGCA GTGGCCCCGC AGAGGGGTCG CAGGAGTCCG TCTGCACTCC TAGTTCTTTA 
CCTGCTGTGC AGGTGCCCAG CAGGGAAGAG GAAGGGTCAG CGGCTGAAGC TGCTGCTTTA 
TCTGAGCAGG GGGAGCTAGG GGAAGTCTGG CAGAGGAGGG GGAGTCTTGA GGGAGCAGCC 
GCTGCTGCTC CTGCTGCTGC TGCCACTGAT AGCCAGCCCC AGGAAGATGG GGATGCTGGG 
GATGCCCAAG GTGCCTGCGA AGGGGCCACT GCCCAGGAGG AGGGCGCCAC TGGAGGTTCT 
CAAACCAATG GCCACCAGCC CTTGAGGTCA CTACCTTCCG TGCGCCAGGA CGTCAGCCGG 
TACCAGAGGG TGGATGAGGC TCTCCCACCA AACTGGGAGG CACGGATTGA CAGCCACGGC 
AGGATCTTCT ATGTGGACCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCAC AGGTCCTGCA GCGATCTAAC TCCATACAGC AAATGGAGCA GCTGAACCGA 
CGTTATCAGA GCATCCGCAG AACCATGACT AATGAGAGGC CTGAGGAAAA TACCAGTGCT 
ATCGATGGGG CAGGGGAGGA AGCCGACTTT CACCAAGCCA GCGCAGACTT CCGTCGGGAG 
AACGTCCTGC CTCACTCTAC CTCCAGATCC AGGCTCACGC TGCTGTTACA GTCACCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AGCGCTATCA GCATAACAGG GACCTCGTGG GATTCCTCAA CATGTTTGCT 
AACAAACAGC TGGAACTACC AAGGGGCTGG GAGATGAAAC ATGATCATCA GGGCAAGGCG 
TTTTTTGTCG ACCACAACTC CCGGACCACC ACGTTCATTG ATCCTCGGCT CCCACTTCAG 
AGCAGTAGGC CCACAAGTGC ATTGGTTCAT CGACAACACC TGACGAGGCA ACGCAGCCAC 
AGTGCGGGTG AGGTAGGAGA AGACTCTCGG CACGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCCAGTACAT TCAATACGGT CAGTAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCCTAC 
AATGACAAAA TTGTTGCGTT TTTGCGTCAA CCCAACATCC TTGAAATTCT GCAAGAGCGT 
CAACCTGATC TTGCCAGAAA CCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACGGAA 
GGGACCCCTG GATTGGTGCG GCTCTCAAGC GATGCAGACC TTGTCATGTT ACTGAGCTTA 
TTCGAGGAAG AGATAATGTC ATATGTGCCT CCTCATGCCT TACTCCACCC CAGCTACTGT 
CAGTCCCCGC GTGGCTCCCC CGTATCATCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGGG CTCCAGCCCC TTACAAGAGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AAACTAAAGG ATACGGACAA GGCCCAGGAA AATTAAAGCT AATTATCCGA 
AGAGATCACT TGCTGGAAGA TGCTTTCAAT CAGATCATGG GCTACTCCAG AAAGGACCTG 
CAGAGGAATA AACTGTACGT CACCTTTGTC GGGGAGGAAG GGTTGGATTA CAGTGGGCCA 
TCCAGAGAGT TCTTCTTCCT GGTATCCCGA GAGCTCTTTA ACCCCTATTA CGGCTTATTT 
GAATATTCAG CCAATGACAC ATATACAGTG CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCATCATG AATGGTTCCG GTTCAGCGGG AGGATCCTCG GCCTTGCGCT CATACACCAG 
TACTTGCTGG ACGCCTTCTT CACGCGACCC TTCTATAAGG CTCTTCTCAG AATCCTGTGT 
GACCTGAGTG ATCTGGAATA CCTTGATGAG GAGTTCCACC AGAGCCTTCA GTGGATGAAA 
GACAATGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAGTT AAAACCAGGG GGAGCCAATA TTCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG AATGGTGAAG TGGAGGATCG AGAGGGGTGT GGTGCAGCAG 
ACAGAGAGCC TAGTTCGTGG TTTCTACGAG GTAGTAGATG CCAGGCTAGT GTCTGTCTTT 
GATGCGAGAG AGCTGGAACT GGTCATTGCC GGCACAGCTG AGATAGATCT CAATGACTGG 
AGAAACAACA CTGAATACAG AGGCGGGTAC CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTAA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTC GCTTCTCTCC GAGGGAGTAA TGGTCCAAGA 
AGATTCTGTG TGGAAAAATG GGGAAAAATC ACCGCTCTTC CCAGAGCCCA CACTTGTTTT 
AACCGCCTGG ATCTGCCTCC ATACCCTTCC TTTTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496066.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496129.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21555
Clone ID Related Accession (Same CDS sequence)	XM_006496066.3 , XM_006496067.1 , XM_006496066.4 , NM_001001883.3 , XM_006496067.2 , XM_006496068.4 , XM_006496068.3
Accession Version	XM_006496066.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496066.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496129.1
CDS	443..5179
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496066.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496066.4

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496067.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496130.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu21555
Clone ID Related Accession (Same CDS sequence)	XM_006496066.3 , XM_006496067.1 , XM_006496066.4 , NM_001001883.3 , XM_006496067.2 , XM_006496068.4 , XM_006496068.3
Accession Version	XM_006496067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496130.1
CDS	419..5155
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496067.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496067.2

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496068.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496131.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X3, mRNA.

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CloneID	OMu21555
Clone ID Related Accession (Same CDS sequence)	XM_006496066.3 , XM_006496067.1 , XM_006496066.4 , NM_001001883.3 , XM_006496067.2 , XM_006496068.4 , XM_006496068.3
Accession Version	XM_006496068.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496068.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496131.1
CDS	275..5011
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496068.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496068.4

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( NM_001001883.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001001883.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1, mRNA.

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CloneID	OMu21555
Clone ID Related Accession (Same CDS sequence)	XM_006496066.3 , XM_006496067.1 , XM_006496066.4 , NM_001001883.3 , XM_006496067.2 , XM_006496068.4 , XM_006496068.3
Accession Version	NM_001001883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124175.2, AC136517.3 and AC150896.6. On Oct 31, 2008 this sequence version replaced NM_001001883.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: AB182244.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001883.1
CDS	95..4831
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001001883.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001883.3

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496066.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496129.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA.

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CloneID	OMu21555
Clone ID Related Accession (Same CDS sequence)	XM_006496066.3 , XM_006496067.1 , XM_006496066.4 , NM_001001883.3 , XM_006496067.2 , XM_006496068.4 , XM_006496068.3
Accession Version	XM_006496066.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496129.1
CDS	568..5304
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496066.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496066.3

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496067.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496130.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu21555
Clone ID Related Accession (Same CDS sequence)	XM_006496066.3 , XM_006496067.1 , XM_006496066.4 , NM_001001883.3 , XM_006496067.2 , XM_006496068.4 , XM_006496068.3
Accession Version	XM_006496067.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496130.1
CDS	106..4842
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496067.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496067.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496068.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496131.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu21555
Clone ID Related Accession (Same CDS sequence)	XM_006496066.3 , XM_006496067.1 , XM_006496066.4 , NM_001001883.3 , XM_006496067.2 , XM_006496068.4 , XM_006496068.3
Accession Version	XM_006496068.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496131.1
CDS	173..4909
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496068.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496068.3

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
TACACACTGA GCCCAGAGAA CCTCCAGAGC CTGGCAGCCC AGAACTCAAT GCCCGAGAAC 
ATGGCCCTAC AGCGGGCCAA CAGTGACACG GATCTGGTGA CTTCTGAGAG CCGCTCCAGC 
CTGACTGCCA GCATGTACGA ATACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATAGGAC TCTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA CTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGACAA 
ATTGTATGGC GAATTGAGCC TGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC 
AAATACTACC ATGGTATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCCGAAGGC ATGGAAGGAG GTGCTTCAGG AAGCCTGCAC 
TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTTCCTACCT GCGCCCACCA TGGGCAGGAG AGAAGGTCCA CTATCATCAG CAACACCACC 
AATCCGATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACTGA TGTCTTAGAA 
ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGGAAA 
CTAACCATTC CAGTGCAGAG GCTGCTGGAG CGGCAGGCTG GGGACCAAAT GCTCAGCTAC 
AACCTTGGCA GACGGCTCCC AGCTGACCAC GTGAGTGGGT ACCTCCAGTT TAAAGTGGAA 
GTTACATCTT CTGCTCACGA AGATGCTTCT CCAGAGGCTG TCGGCACCAT CCTCGGAGTC 
CACACTGTGA ATGGAGACCT GGGCAGCCCT TCCGATGAGG AGGATATGCC AGGCAGCCAT 
CATGACAGTA CTATCTGTGC TAATGGGCCA GTGTCCGAGG ACAGTGTTGC TGATGGCACC 
CCTAAGCATT CTTTCAGGAC TAGCTCTACC CTGGAGATCG ACACGGAGGA CTTGATCTCT 
ACTTCTTCTA GAAATTCACC CCCCAGGGGC CGCCAGGATT CCCTCAATGA TTACTTAGAT 
GCTATTGAAC ACAATGGCCC CGCCAGGCCA GGGGCAGCCT CCTCCTCTGA GCGGTCCATG 
GGAGCCTCTC CTAAACTGAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCGATGCTT 
CATATTGACT CAGACGAAGA AGACCATGAG TTTCAGCAAG ACTTGGGCTA CCCATCTTCT 
TTGGAGGAGG AGGGAGGCTT GATCATGTGC AGCAGGGCGT CGAGAATTGA TGATGGGAGC 
CTGACATCTC AGACCAAACC AGAGGATGAC AACCCTGTGG AGAATGAGGA TGCCTCTATA 
CATGAAACTG CATCCTTAGA AGAGAGGCTG GAGAATCTTC CAGAGGTTGC AGATGGCTCC 
TTGCCATCAA GTACAGCCCC TGATGAAAAT GAAGCCAACC TGGAGCCTCA ACCCAGTGCA 
GACCAGGGTA GCACTGAATT GTGTAGCTCT CAAGAAGTAG ATCAGCCCAC AAGTGGTGCA 
GATGCAGGAG CTTCTGACAC ATCTGGAGGG AGCCGCAGAG CTGCCAGTGA GACCGAGTCC 
CTGGACCAGG GATCTGAACC TTCCCAGGTG TCCTCTGAGA CAGAACCCAG TGACCCTGCA 
AGGACAGAGA GTGTGAGTGA GGCCAGTACC AGACCTGAGG GGGAGAGTGA CCCGGAAGGC 
GCAGACAGCT CATGCAATGA GAGCGTGACT ACACAGCTGT CCTCCGTGGA GACACGGTGC 
TCATCTCTAG AGAGTGCGCG ATTTCCAGAA ACCCCCGCCT TTTCTTCTCA GGAGGAAGAA 
GATGGGGCCT GTGCAGCTGA GCCCACCAGC AGTGGCCCCG CAGAGGGGTC GCAGGAGTCC 
GTCTGCACTC CTAGTTCTTT ACCTGCTGTG CAGGTGCCCA GCAGGGAAGA GGAAGGGTCA 
GCGGCTGAAG CTGCTGCTTT ATCTGAGCAG GGGGAGCTAG GGGAAGTCTG GCAGAGGAGG 
GGGAGTCTTG AGGGAGCAGC CGCTGCTGCT CCTGCTGCTG CTGCCACTGA TAGCCAGCCC 
CAGGAAGATG GGGATGCTGG GGATGCCCAA GGTGCCTGCG AAGGGGCCAC TGCCCAGGAG 
GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCACCAGC CCTTGAGGTC ACTACCTTCC 
GTGCGCCAGG ACGTCAGCCG GTACCAGAGG GTGGATGAGG CTCTCCCACC AAACTGGGAG 
GCACGGATTG ACAGCCACGG CAGGATCTTC TATGTGGACC ATGTAAACAG AACCACAACG 
TGGCAGCGGC CCACAGCTCC CCCTGCCCCA CAGGTCCTGC AGCGATCTAA CTCCATACAG 
CAAATGGAGC AGCTGAACCG ACGTTATCAG AGCATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAGG AAGCCGACTT TCACCAAGCC 
AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCTCACTCTA CCTCCAGATC CAGGCTCACG 
CTGCTGTTAC AGTCACCCCC CGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTT GAGCGCTATC AGCATAACAG GGACCTCGTG 
GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA 
CATGATCATC AGGGCAAGGC GTTTTTTGTC GACCACAACT CCCGGACCAC CACGTTCATT 
GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACAAGTG CATTGGTTCA TCGACAACAC 
CTGACGAGGC AACGCAGCCA CAGTGCGGGT GAGGTAGGAG AAGACTCTCG GCACGCAGGA 
CCACCAGTTC TTCCCAGGCC ATCCAGTACA TTCAATACGG TCAGTAGGCC GCAGTACCAG 
GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCCAACATC 
CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG 
ATCCAATTTA TCCGAACGGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG CGATGCAGAC 
CTTGTCATGT TACTGAGCTT ATTCGAGGAA GAGATAATGT CATATGTGCC TCCTCATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CCGTATCATC TCCCCAGAAC 
TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCAGCCC CTTACAAGAG AGATTTTGAA 
GCCAAACTAA GGAACTTTTA CAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA 
AAATTAAAGC TAATTATCCG AAGAGATCAC TTGCTGGAAG ATGCTTTCAA TCAGATCATG 
GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA 
GGGTTGGATT ACAGTGGGCC ATCCAGAGAG TTCTTCTTCC TGGTATCCCG AGAGCTCTTT 
AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATATACAGT GCAAATAAGT 
CCCATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTCC GGTTCAGCGG GAGGATCCTC 
GGCCTTGCGC TCATACACCA GTACTTGCTG GACGCCTTCT TCACGCGACC CTTCTATAAG 
GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC 
CAGAGCCTTC AGTGGATGAA AGACAATGAC ATCCATGACA TCCTGGACCT CACGTTCACT 
GTGAATGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT 
ATTCCAGTCA CAGAGAAGAA CAAGAAGGAG TACATCGAGA GAATGGTGAA GTGGAGGATC 
GAGAGGGGTG TGGTGCAGCA GACAGAGAGC CTAGTTCGTG GTTTCTACGA GGTAGTAGAT 
GCCAGGCTAG TGTCTGTCTT TGATGCGAGA GAGCTGGAAC TGGTCATTGC CGGCACAGCT 
GAGATAGATC TCAATGACTG GAGAAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTGGAAAGAT TCAACAATGA ACAACGACTA 
AGGTTGTTAC AGTTTGTTAC AGGCACATCC AGCATTCCCT ATGAAGGATT CGCTTCTCTC 
CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT 
CCCAGAGCCC ACACTTGTTT TAACCGCCTG GATCTGCCTC CATACCCTTC CTTTTCCATG 
CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_011238537.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236839.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X5, mRNA.

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CloneID	OMu27471
Clone ID Related Accession (Same CDS sequence)	XM_011238537.2 , XM_006496069.4 , XM_011238537.3 , XM_006496069.3
Accession Version	XM_011238537.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236839.1
CDS	380..4723
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011238537.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238537.2

ORF Insert Sequence:

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw2 ( XM_006496069.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496132.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X4, mRNA.

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CloneID	OMu27471
Clone ID Related Accession (Same CDS sequence)	XM_011238537.2 , XM_006496069.4 , XM_011238537.3 , XM_006496069.3
Accession Version	XM_006496069.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496069.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496132.1
CDS	824..5167
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006496069.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496069.3

ORF Insert Sequence:

ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GTATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGTATTAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC CGAAGGCATG 
GAAGGAGGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT 
CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTT CCTACCTGCG CCCACCATGG GCAGGAGAGA 
AGGTCCACTA TCATCAGCAA CACCACCAAT CCGATCTGGC ACAGAGAGAA ATACTCCTTT 
TTTGCACTTT TGACTGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG 
CCCATCATCA AACGCTTCCT GGGGAAACTA ACCATTCCAG TGCAGAGGCT GCTGGAGCGG 
CAGGCTGGGG ACCAAATGCT CAGCTACAAC CTTGGCAGAC GGCTCCCAGC TGACCACGTG 
AGTGGGTACC TCCAGTTTAA AGTGGAAGTT ACATCTTCTG CTCACGAAGA TGCTTCTCCA 
GAGGCTGTCG GCACCATCCT CGGAGTCCAC ACTGTGAATG GAGACCTGGG CAGCCCTTCC 
GATGAGGAGG ATATGCCAGG CAGCCATCAT GACAGTACTA TCTGTGCTAA TGGGCCAGTG 
TCCGAGGACA GTGTTGCTGA TGGCACCCCT AAGCATTCTT TCAGGACTAG CTCTACCCTG 
GAGATCGACA CGGAGGACTT GATCTCTACT TCTTCTAGAA ATTCACCCCC CAGGGGCCGC 
CAGGATTCCC TCAATGATTA CTTAGATGCT ATTGAACACA ATGGCCCCGC CAGGCCAGGG 
GCAGCCTCCT CCTCTGAGCG GTCCATGGGA GCCTCTCCTA AACTGAGGAG TAGTTTTCCC 
ACTGACACAA GACTCAATGC GATGCTTCAT ATTGACTCAG ACGAAGAAGA CCATGAGTTT 
CAGCAAGACT TGGGCTACCC ATCTTCTTTG GAGGAGGAGG GAGGCTTGAT CATGTGCAGC 
AGGGCGTCGA GAATTGATGA TGGGAGCCTG ACATCTCAGA CCAAACCAGA GGATGACAAC 
CCTGTGGAGA ATGAGGATGC CTCTATACAT GAAACTGCAT CCTTAGAAGA GAGGCTGGAG 
AATCTTCCAG AGGTTGCAGA TGGCTCCTTG CCATCAAGTA CAGCCCCTGA TGAAAATGAA 
GCCAACCTGG AGCCTCAACC CAGTGCAGAC CAGGGTAGCA CTGAATTGTG TAGCTCTCAA 
GAAGTAGATC AGCCCACAAG TGGTGCAGAT GCAGGAGCTT CTGACACATC TGGAGGGAGC 
CGCAGAGCTG CCAGTGAGAC CGAGTCCCTG GACCAGGGAT CTGAACCTTC CCAGGTGTCC 
TCTGAGACAG AACCCAGTGA CCCTGCAAGG ACAGAGAGTG TGAGTGAGGC CAGTACCAGA 
CCTGAGGGGG AGAGTGACCC GGAAGGCGCA GACAGCTCAT GCAATGAGAG CGTGACTACA 
CAGCTGTCCT CCGTGGAGAC ACGGTGCTCA TCTCTAGAGA GTGCGCGATT TCCAGAAACC 
CCCGCCTTTT CTTCTCAGGA GGAAGAAGAT GGGGCCTGTG CAGCTGAGCC CACCAGCAGT 
GGCCCCGCAG AGGGGTCGCA GGAGTCCGTC TGCACTCCTA GTTCTTTACC TGCTGTGCAG 
GTGCCCAGCA GGGAAGAGGA AGGGTCAGCG GCTGAAGCTG CTGCTTTATC TGAGCAGGGG 
GAGCTAGGGG AAGTCTGGCA GAGGAGGGGG AGTCTTGAGG GAGCAGCCGC TGCTGCTCCT 
GCTGCTGCTG CCACTGATAG CCAGCCCCAG GAAGATGGGG ATGCTGGGGA TGCCCAAGGT 
GCCTGCGAAG GGGCCACTGC CCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC 
CACCAGCCCT TGAGGTCACT ACCTTCCGTG CGCCAGGACG TCAGCCGGTA CCAGAGGGTG 
GATGAGGCTC TCCCACCAAA CTGGGAGGCA CGGATTGACA GCCACGGCAG GATCTTCTAT 
GTGGACCATG TAAACAGAAC CACAACGTGG CAGCGGCCCA CAGCTCCCCC TGCCCCACAG 
GTCCTGCAGC GATCTAACTC CATACAGCAA ATGGAGCAGC TGAACCGACG TTATCAGAGC 
ATCCGCAGAA CCATGACTAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCT 
CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CACCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAG 
CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG 
GAACTACCAA GGGGCTGGGA GATGAAACAT GATCATCAGG GCAAGGCGTT TTTTGTCGAC 
CACAACTCCC GGACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC 
ACAAGTGCAT TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGCCACAG TGCGGGTGAG 
GTAGGAGAAG ACTCTCGGCA CGCAGGACCA CCAGTTCTTC CCAGGCCATC CAGTACATTC 
AATACGGTCA GTAGGCCGCA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT 
GTTGCGTTTT TGCGTCAACC CAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT 
GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACGGAAGG GACCCCTGGA 
TTGGTGCGGC TCTCAAGCGA TGCAGACCTT GTCATGTTAC TGAGCTTATT CGAGGAAGAG 
ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT 
GGCTCCCCCG TATCATCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT 
CCAGCCCCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTACAG GAAGTTAGAA 
ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGCTAA TTATCCGAAG AGATCACTTG 
CTGGAAGATG CTTTCAATCA GATCATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA 
CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGG TATCCCGAGA GCTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC 
AATGACACAT ATACAGTGCA AATAAGTCCC ATGTCTGCCT TTGTAGACAA TCATCATGAA 
TGGTTCCGGT TCAGCGGGAG GATCCTCGGC CTTGCGCTCA TACACCAGTA CTTGCTGGAC 
GCCTTCTTCA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT 
CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AATGAAGAAG TTTTTGGGCA GATTACTGAA 
CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTCACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGAA TGGTGAAGTG GAGGATCGAG AGGGGTGTGG TGCAGCAGAC AGAGAGCCTA 
GTTCGTGGTT TCTACGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCGAGAGAG 
CTGGAACTGG TCATTGCCGG CACAGCTGAG ATAGATCTCA ATGACTGGAG AAACAACACT 
GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTCGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG 
GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCCCACA CTTGTTTTAA CCGCCTGGAT 
CTGCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG 
ACCAGTACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Hecw2
Entrez Gene ID	329152
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Synonyms	A730039N16Rik,D030049F17Rik,Gm971,Nedl2,mKIAA1301
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase HECW2 HECT, C2 and WW domain-containing protein 2 HECT-type E3 ubiquitin transferase HECW2 NEDD4-like E3 ubiquitin-protein ligase 2 NEDD4-related E3 ubiquitin ligase NEDL2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C1.1

	LOC697155	XP_002808063.1
Mus musculus (house mouse)	Hecw2	NP_001001883.1
Gallus gallus (chicken)	HECW2	XP_421906.2
Bos taurus (cattle)	HECW2	XP_002685520.2
Rattus norvegicus (Norway rat)	Hecw2	NP_001101688.1
Pan troglodytes (chimpanzee)	HECW2	XP_516001.2
Canis lupus familiaris (dog)	HECW2	XP_005640790.1
Danio rerio (zebrafish)	hecw2	XP_003197660.2
Homo sapiens (human)	HECW2	NP_065811.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Hecw2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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