HECW2 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_009443908.2	XP_009442183.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_009443907.3	XP_009442182.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_516001.5	XP_516001.2	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_009443906.2	XP_009442181.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_024354993.1	XP_024210761.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_016950255.1	XP_016805744.1	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_016950255.2	XP_016805744.1	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_009443907.2	XP_009442182.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_516001.4	XP_516001.2	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_009443910.2	XP_009442185.1	E3 ubiquitin-protein ligase HECW2 isoform X4
XM_009443908.3	XP_009442183.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_009443910.3	XP_009442185.1	E3 ubiquitin-protein ligase HECW2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc80472	XM_009443910.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc80469	XM_009443906.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80469	XM_009443907.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80469	XM_009443908.3 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80470	XM_516001.5 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80470	XM_024354993.1 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80471	XM_016950255.2 Latest version!	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OPc80471	XM_016950255.1	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80469	XM_009443907.2	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80470	XM_516001.4	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80469	XM_009443908.2	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc80472	XM_009443910.2	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

HECW2 ( XM_009443910.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442185.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc80472
Clone ID Related Accession (Same CDS sequence)	XM_009443910.2 , XM_009443910.3
Accession Version	XM_009443910.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009443910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATAAAAAT CTGTTTTAAA TATTACCACG GCATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGCATCAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAGGGAGGTG CTTCAGGAAA CCTGCATTCT CGAAAACTTG TTAGCTTTAC ATTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAAGAGAGA 
CGGTCTACTA TCATCAGTAA CACCACCAAT CCAATTTGGC ACCGAGAGAA ATATTCCTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCCATCATCA AGCGTTTTCT GGGGAAACTA ACCATTCCAG TCCAGAGGCT GCTGGAGCGA 
CAAGCCATCG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAAAGTGGAG GTTACATCTT CTGTTCATGA AGAAATGCTG 
TTTTCTCATT CAGATGCCTC TCCAGAAGCT GTTGGCACAA TACTTGGAGT CAATTCTGTG 
AATGGAGACT TAGGTAGCCC TTCCGATGAC GAGGACATGC CAGGGAGCCA TCACGACAGC 
CAGGTGTGCT CTAATGGGCC AGTTTCTGAG GACAGTGCTG CCGATGGAAC CCCCAAGCAT 
TCACTCAGGA CTAGCTCCAC TCTGGAAATA GACACAGAGG AATTAACCTC TACTTCTTCA 
AGGACCTCAC CTCCCAGAGG ACGTCAGGAT TCACTCAATG ATTACTTAGA TGCTATCGAA 
CACAATGGCC ACTCCAGGCC GGGGACAGCG ACCTGCTCTG AGAGGTCCAT GGGTGCCTCT 
CCGAAACTGA GGAGTAGTTT TCCCACTGAC ACAAGACTCA ATGCCATGCT TCATATTGAT 
TCAGATGAAG AAGATCACGA GTTCCAGCAA GACCTGGGTT ACCCATCTTC CTTGGAGGAG 
GAGGGAGGCC TGATCATGTT TAGCAGGGCA TCCAGAGCTG ATGATGGAAG CCTGACATCT 
CAGACAAAGC TGGAGGACAA TCCTGTTGAG AATGAGGAAG CCTCCATACA CGAAGCTGCT 
TCCTTTGAGG ATAAGCCAGA AAATCTTCCA GAGCTTGCAG AGAGCTCCTT ACCTGCAGGC 
CCAGCCCCAG AGGAAGGTGA AGGCGGCCCA GAGTCTCAAC CCAGTGCTGA CCAGGGCAGT 
GCTGAACTGT GTGGCTCTCA AGAGGTAGAT CAGCCCACAA GTGGGGCAGA CACGGGGACT 
TCTGATGCAT CAGGAGGAAG CCGAAGAGCC GTCAGTGAGA CCGAGTCTCT CGATCAGGGC 
TCTGAGCCTT CCCAGGTGTC CTCTGAAACA GAACCCAGTG ATCCTGCCAG GACAGAGAGC 
GTGAGCGAAG CCAGCACCAG GCCTGAGGGA GAGAGTGACC TGGAATGCGC TGACAGCTCC 
TGCAATGAGA GTGTGACCAC GCAGCTGTCC TCTGTGGACA CACGGTGCTC CTCTCTTGAG 
AGCGCACGGT TTCCTGAAAC CCCAGCCTTT TCTTCTCAGG AGGAGGAAGA CGGAGCCTGT 
GCAGCAGAGC CCACCAGCAG TGGCCCTGCC GAAGGGTCGC AGGAATCCGT GTGCACTGCT 
GGTTCTTTAC CTGTGGTACA GGTGCCCAGT GGGGAGGATG AAGGGCCAGG GGCAGAATCG 
GCCACTGTAC CTGACCAGGA GTTGCTGGGG GAGGTCTGGC AGCGGAGGGG GAGCCTGGAG 
GGAGCTGCAG CTGCTGCTGA GAGCCCACTG CAAGAAGAAG GCAGTGCTGG GGAGGCCCAA 
GGCACCTGTG AAGGGGCAAC TGCCCAGGAG GAGGGCGCTA CTGGAGGTTC TCAAGCCAAC 
GGCCACCAGC CACTGCGAAC ACTACCTTCA GTGCGCCAGG ATGTTAGCCG GTACCAGAGG 
GTGGACGAGG CTCTCCCACC AAACTGGGAG GCACGCATTG ACAGCCACGG CAGGATCTTC 
TACGTGGATC ACGTAAACAG AACCACGACG TGGCAGCGAC CGACAGCTCC CCCAGCCCCA 
CAGGTGCTGC AGAGATCTAA CTCCATACAG CAAATGGAGC AGCTGAACCG GCGATATCAG 
AGTATCCGCA GAACCATGAC TAATGAGAGG CCTGAGGAAA ATACCAATGC TATTGATGGG 
GCAGGGGAGG AAGCTGACTT TCACCAAGCC AGTGCAGATT TCCGACGGGA GAACATCCTG 
CCTCACTCTA CCTCCAGATC CAGGCTCACG TTGCTGTTAC AGTCTCCCCC CGTGAAGTTC 
CTCATCAGCC CAGAGTTCTT CACCGTGCTG CATTCTAACC CTAGTGCCTA CCGCATGTTT 
ACAAACAACA CGTGTTTGAA GCACATGATC ACCAAAGTCC GGAGGGACAC CCACCACTTT 
GAACGCTACC AGCATAACCG GGACCTTGTG GGATTCCTCA ACATGTTCGC GAACAAACAG 
CTAGAGCTGC CGCGGGGATG GGAAATGAAA CATGATCACC AGGGCAAGGC ATTCTTTGTG 
GACCACAACT CCCGCACCAC CACTTTCATT GATCCCCGGC TCCCACTTCA GAGCAGTAGA 
CCCACAAGTG CGCTGGTTCA TCGGCAACAC CTGACAAGGC AACGCAGCCA CAGTGCGGGT 
GAGGTAGGAG AAGATTCTCG ACATGCAGGA CCACCAGTTC TTCCCAGGCC ATCCAGTACA 
TTCAATACAG TCAGTAGGCC ACAGTACCAG GACATGGTTC CAGTGGCTTA CAATGACAAG 
ATTGTTGCAT TTCTACGTCA ACCCAATATC TTTGAAATTC TACAGGAGCG TCAACCTGAT 
CTTACCAGGA ATCACTCACT CAGGGAGAAG ATCCAATTTA TCCGAACTGA AGGGACTCCA 
GGATTGGTGC GCCTTTCAAG CGATGCAGAC CTTGTTATGT TGCTGAGCTT ATTTGAAGAA 
GAGATAATGT CATACGTGCC TCCACATGCC TTACTCCACC CCAGCTACTG TCAGTCCCCA 
CGTGGCTCTC CCGTGTCATC TCCTCAGAAC TCGCCAGGTA CCCAGCGTGC CAATGCCCGG 
GCTCCAGCCC CTTACAAGCG GGATTTCGAA GCCAAACTGA GGAACTTTTA CAGGAAGTTA 
GAGACTAAAG GATATGGACA AGGCCCAGGG AAGTTAAAGT TAATTATCCG AAGAGATCAC 
TTACTAGAAG ATGCTTTTAA TCAGATTATG GGCTACTCCA GAAAAGACCT GCAGAGAAAT 
AAGCTGTATG TCACCTTCGT CGGGGAGGAA GGGCTGGATT ACAGTGGGCC TTCTAGAGAG 
TTTTTCTTCC TGGTATCCAG AGAACTCTTT AACCCATATT ATGGCTTATT TGAATATTCA 
GCCAATGACA CATACACAGT ACAAATAAGT CCTATGTCTG CTTTTGTAGA CAATCACCAT 
GAATGGTTCC GATTCAGTGG TAGGATCCTT GGTCTTGCAC TAATACACCA GTATTTGTTG 
GATGCCTTCT TCACACGGCC CTTTTATAAG GCTCTTCTCA GAATTCTATG TGACCTGAGT 
GACCTAGAAT ACCTTGATGA AGAGTTCCAT CAGAGCCTGC AGTGGATGAA AGACAATGAT 
ATCCATGACA TCCTAGACCT CACGTTCACT GTGAACGAAG AAGTATTTGG GCAGATAACT 
GAACGAGAAT TAAAGCCAGG GGGTGCCAAT ATCCCAGTTA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGA GGATGGTGAA GTGGAGGATT GAGAGGGGTG TTGTACAGCA AACAGAGAGC 
TTAGTGCGTG GCTTCTATGA GGTGGTGGAT GCCAGGCTGG TATCTGTTTT TGATGCAAGA 
GAACTGGAAT TGGTCATCGC AGGCACAGCT GAAATAGACC TAAGTGATTG GAGAAACAAC 
ACAGAATATA GAGGAGGATA CCATGACAAT CATATTGTAA TTCGGTGGTT CTGGGCTGCA 
GTGGAAAGAT TCAACAATGA ACAACGACTA AGGTTGTTAC AGTTTGTTAC AGGCACATCC 
AGCATTCCCT ATGAAGGATT TGCTTCACTC CGAGGGAGTA ACGGCCCAAG AAGATTCTGT 
GTGGAGAAAT GGGGGAAAAT CACTGCTCTT CCCAGAGCGC ATACATGTTT TAACCGTCTG 
GATCTGCCTC CCTACCCATC CTTTTCCATG CTTTATGAAA AACTGTTGAC AGCAGTTGAA 
GAAACCAGTA CTTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442185.1
CDS	479..4825
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009443910.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009443910.2

ORF Insert Sequence:

ATGGAACCGG AGATAAAAAT CTGTTTTAAA TATTACCACG GCATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGCATCAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAGGGAGGTG CTTCAGGAAA CCTGCATTCT CGAAAACTTG TTAGCTTTAC ATTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAAGAGAGA 
CGGTCTACTA TCATCAGTAA CACCACCAAT CCAATTTGGC ACCGAGAGAA ATATTCCTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCCATCATCA AGCGTTTTCT GGGGAAACTA ACCATTCCAG TCCAGAGGCT GCTGGAGCGA 
CAAGCCATCG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAAAGTGGAG GTTACATCTT CTGTTCATGA AGAAATGCTG 
TTTTCTCATT CAGATGCCTC TCCAGAAGCT GTTGGCACAA TACTTGGAGT CAATTCTGTG 
AATGGAGACT TAGGTAGCCC TTCCGATGAC GAGGACATGC CAGGGAGCCA TCACGACAGC 
CAGGTGTGCT CTAATGGGCC AGTTTCTGAG GACAGTGCTG CCGATGGAAC CCCCAAGCAT 
TCACTCAGGA CTAGCTCCAC TCTGGAAATA GACACAGAGG AATTAACCTC TACTTCTTCA 
AGGACCTCAC CTCCCAGAGG ACGTCAGGAT TCACTCAATG ATTACTTAGA TGCTATCGAA 
CACAATGGCC ACTCCAGGCC GGGGACAGCG ACCTGCTCTG AGAGGTCCAT GGGTGCCTCT 
CCGAAACTGA GGAGTAGTTT TCCCACTGAC ACAAGACTCA ATGCCATGCT TCATATTGAT 
TCAGATGAAG AAGATCACGA GTTCCAGCAA GACCTGGGTT ACCCATCTTC CTTGGAGGAG 
GAGGGAGGCC TGATCATGTT TAGCAGGGCA TCCAGAGCTG ATGATGGAAG CCTGACATCT 
CAGACAAAGC TGGAGGACAA TCCTGTTGAG AATGAGGAAG CCTCCATACA CGAAGCTGCT 
TCCTTTGAGG ATAAGCCAGA AAATCTTCCA GAGCTTGCAG AGAGCTCCTT ACCTGCAGGC 
CCAGCCCCAG AGGAAGGTGA AGGCGGCCCA GAGTCTCAAC CCAGTGCTGA CCAGGGCAGT 
GCTGAACTGT GTGGCTCTCA AGAGGTAGAT CAGCCCACAA GTGGGGCAGA CACGGGGACT 
TCTGATGCAT CAGGAGGAAG CCGAAGAGCC GTCAGTGAGA CCGAGTCTCT CGATCAGGGC 
TCTGAGCCTT CCCAGGTGTC CTCTGAAACA GAACCCAGTG ATCCTGCCAG GACAGAGAGC 
GTGAGCGAAG CCAGCACCAG GCCTGAGGGA GAGAGTGACC TGGAATGCGC TGACAGCTCC 
TGCAATGAGA GTGTGACCAC GCAGCTGTCC TCTGTGGACA CACGGTGCTC CTCTCTTGAG 
AGCGCACGGT TTCCTGAAAC CCCAGCCTTT TCTTCTCAGG AGGAGGAAGA CGGAGCCTGT 
GCAGCAGAGC CCACCAGCAG TGGCCCTGCC GAAGGGTCGC AGGAATCCGT GTGCACTGCT 
GGTTCTTTAC CTGTGGTACA GGTGCCCAGT GGGGAGGATG AAGGGCCAGG GGCAGAATCG 
GCCACTGTAC CTGACCAGGA GTTGCTGGGG GAGGTCTGGC AGCGGAGGGG GAGCCTGGAG 
GGAGCTGCAG CTGCTGCTGA GAGCCCACTG CAAGAAGAAG GCAGTGCTGG GGAGGCCCAA 
GGCACCTGTG AAGGGGCAAC TGCCCAGGAG GAGGGCGCTA CTGGAGGTTC TCAAGCCAAC 
GGCCACCAGC CACTGCGAAC ACTACCTTCA GTGCGCCAGG ATGTTAGCCG GTACCAGAGG 
GTGGACGAGG CTCTCCCACC AAACTGGGAG GCACGCATTG ACAGCCACGG CAGGATCTTC 
TACGTGGATC ACGTAAACAG AACCACGACG TGGCAGCGAC CGACAGCTCC CCCAGCCCCA 
CAGGTGCTGC AGAGATCTAA CTCCATACAG CAAATGGAGC AGCTGAACCG GCGATATCAG 
AGTATCCGCA GAACCATGAC TAATGAGAGG CCTGAGGAAA ATACCAATGC TATTGATGGG 
GCAGGGGAGG AAGCTGACTT TCACCAAGCC AGTGCAGATT TCCGACGGGA GAACATCCTG 
CCTCACTCTA CCTCCAGATC CAGGCTCACG TTGCTGTTAC AGTCTCCCCC CGTGAAGTTC 
CTCATCAGCC CAGAGTTCTT CACCGTGCTG CATTCTAACC CTAGTGCCTA CCGCATGTTT 
ACAAACAACA CGTGTTTGAA GCACATGATC ACCAAAGTCC GGAGGGACAC CCACCACTTT 
GAACGCTACC AGCATAACCG GGACCTTGTG GGATTCCTCA ACATGTTCGC GAACAAACAG 
CTAGAGCTGC CGCGGGGATG GGAAATGAAA CATGATCACC AGGGCAAGGC ATTCTTTGTG 
GACCACAACT CCCGCACCAC CACTTTCATT GATCCCCGGC TCCCACTTCA GAGCAGTAGA 
CCCACAAGTG CGCTGGTTCA TCGGCAACAC CTGACAAGGC AACGCAGCCA CAGTGCGGGT 
GAGGTAGGAG AAGATTCTCG ACATGCAGGA CCACCAGTTC TTCCCAGGCC ATCCAGTACA 
TTCAATACAG TCAGTAGGCC ACAGTACCAG GACATGGTTC CAGTGGCTTA CAATGACAAG 
ATTGTTGCAT TTCTACGTCA ACCCAATATC TTTGAAATTC TACAGGAGCG TCAACCTGAT 
CTTACCAGGA ATCACTCACT CAGGGAGAAG ATCCAATTTA TCCGAACTGA AGGGACTCCA 
GGATTGGTGC GCCTTTCAAG CGATGCAGAC CTTGTTATGT TGCTGAGCTT ATTTGAAGAA 
GAGATAATGT CATACGTGCC TCCACATGCC TTACTCCACC CCAGCTACTG TCAGTCCCCA 
CGTGGCTCTC CCGTGTCATC TCCTCAGAAC TCGCCAGGTA CCCAGCGTGC CAATGCCCGG 
GCTCCAGCCC CTTACAAGCG GGATTTCGAA GCCAAACTGA GGAACTTTTA CAGGAAGTTA 
GAGACTAAAG GATATGGACA AGGCCCAGGG AAGTTAAAGT TAATTATCCG AAGAGATCAC 
TTACTAGAAG ATGCTTTTAA TCAGATTATG GGCTACTCCA GAAAAGACCT GCAGAGAAAT 
AAGCTGTATG TCACCTTCGT CGGGGAGGAA GGGCTGGATT ACAGTGGGCC TTCTAGAGAG 
TTTTTCTTCC TGGTATCCAG AGAACTCTTT AACCCATATT ATGGCTTATT TGAATATTCA 
GCCAATGACA CATACACAGT ACAAATAAGT CCTATGTCTG CTTTTGTAGA CAATCACCAT 
GAATGGTTCC GATTCAGTGG TAGGATCCTT GGTCTTGCAC TAATACACCA GTATTTGTTG 
GATGCCTTCT TCACACGGCC CTTTTATAAG GCTCTTCTCA GAATTCTATG TGACCTGAGT 
GACCTAGAAT ACCTTGATGA AGAGTTCCAT CAGAGCCTGC AGTGGATGAA AGACAATGAT 
ATCCATGACA TCCTAGACCT CACGTTCACT GTGAACGAAG AAGTATTTGG GCAGATAACT 
GAACGAGAAT TAAAGCCAGG GGGTGCCAAT ATCCCAGTTA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGA GGATGGTGAA GTGGAGGATT GAGAGGGGTG TTGTACAGCA AACAGAGAGC 
TTAGTGCGTG GCTTCTATGA GGTGGTGGAT GCCAGGCTGG TATCTGTTTT TGATGCAAGA 
GAACTGGAAT TGGTCATCGC AGGCACAGCT GAAATAGACC TAAGTGATTG GAGAAACAAC 
ACAGAATATA GAGGAGGATA CCATGACAAT CATATTGTAA TTCGGTGGTT CTGGGCTGCA 
GTGGAAAGAT TCAACAATGA ACAACGACTA AGGTTGTTAC AGTTTGTTAC AGGCACATCC 
AGCATTCCCT ATGAAGGATT TGCTTCACTC CGAGGGAGTA ACGGCCCAAG AAGATTCTGT 
GTGGAGAAAT GGGGGAAAAT CACTGCTCTT CCCAGAGCGC ATACATGTTT TAACCGTCTG 
GATCTGCCTC CCTACCCATC CTTTTCCATG CTTTATGAAA AACTGTTGAC AGCAGTTGAA 
GAAACCAGTA CTTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_009443910.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442185.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPc80472
Clone ID Related Accession (Same CDS sequence)	XM_009443910.2 , XM_009443910.3
Accession Version	XM_009443910.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009443910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATAAAAAT CTGTTTTAAA TATTACCACG GCATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGCATCAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAGGGAGGTG CTTCAGGAAA CCTGCATTCT CGAAAACTTG TTAGCTTTAC ATTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAAGAGAGA 
CGGTCTACTA TCATCAGTAA CACCACCAAT CCAATTTGGC ACCGAGAGAA ATATTCCTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCCATCATCA AGCGTTTTCT GGGGAAACTA ACCATTCCAG TCCAGAGGCT GCTGGAGCGA 
CAAGCCATCG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAAAGTGGAG GTTACATCTT CTGTTCATGA AGAAATGCTG 
TTTTCTCATT CAGATGCCTC TCCAGAAGCT GTTGGCACAA TACTTGGAGT CAATTCTGTG 
AATGGAGACT TAGGTAGCCC TTCCGATGAC GAGGACATGC CAGGGAGCCA TCACGACAGC 
CAGGTGTGCT CTAATGGGCC AGTTTCTGAG GACAGTGCTG CCGATGGAAC CCCCAAGCAT 
TCACTCAGGA CTAGCTCCAC TCTGGAAATA GACACAGAGG AATTAACCTC TACTTCTTCA 
AGGACCTCAC CTCCCAGAGG ACGTCAGGAT TCACTCAATG ATTACTTAGA TGCTATCGAA 
CACAATGGCC ACTCCAGGCC GGGGACAGCG ACCTGCTCTG AGAGGTCCAT GGGTGCCTCT 
CCGAAACTGA GGAGTAGTTT TCCCACTGAC ACAAGACTCA ATGCCATGCT TCATATTGAT 
TCAGATGAAG AAGATCACGA GTTCCAGCAA GACCTGGGTT ACCCATCTTC CTTGGAGGAG 
GAGGGAGGCC TGATCATGTT TAGCAGGGCA TCCAGAGCTG ATGATGGAAG CCTGACATCT 
CAGACAAAGC TGGAGGACAA TCCTGTTGAG AATGAGGAAG CCTCCATACA CGAAGCTGCT 
TCCTTTGAGG ATAAGCCAGA AAATCTTCCA GAGCTTGCAG AGAGCTCCTT ACCTGCAGGC 
CCAGCCCCAG AGGAAGGTGA AGGCGGCCCA GAGTCTCAAC CCAGTGCTGA CCAGGGCAGT 
GCTGAACTGT GTGGCTCTCA AGAGGTAGAT CAGCCCACAA GTGGGGCAGA CACGGGGACT 
TCTGATGCAT CAGGAGGAAG CCGAAGAGCC GTCAGTGAGA CCGAGTCTCT CGATCAGGGC 
TCTGAGCCTT CCCAGGTGTC CTCTGAAACA GAACCCAGTG ATCCTGCCAG GACAGAGAGC 
GTGAGCGAAG CCAGCACCAG GCCTGAGGGA GAGAGTGACC TGGAATGCGC TGACAGCTCC 
TGCAATGAGA GTGTGACCAC GCAGCTGTCC TCTGTGGACA CACGGTGCTC CTCTCTTGAG 
AGCGCACGGT TTCCTGAAAC CCCAGCCTTT TCTTCTCAGG AGGAGGAAGA CGGAGCCTGT 
GCAGCAGAGC CCACCAGCAG TGGCCCTGCC GAAGGGTCGC AGGAATCCGT GTGCACTGCT 
GGTTCTTTAC CTGTGGTACA GGTGCCCAGT GGGGAGGATG AAGGGCCAGG GGCAGAATCG 
GCCACTGTAC CTGACCAGGA GTTGCTGGGG GAGGTCTGGC AGCGGAGGGG GAGCCTGGAG 
GGAGCTGCAG CTGCTGCTGA GAGCCCACTG CAAGAAGAAG GCAGTGCTGG GGAGGCCCAA 
GGCACCTGTG AAGGGGCAAC TGCCCAGGAG GAGGGCGCTA CTGGAGGTTC TCAAGCCAAC 
GGCCACCAGC CACTGCGAAC ACTACCTTCA GTGCGCCAGG ATGTTAGCCG GTACCAGAGG 
GTGGACGAGG CTCTCCCACC AAACTGGGAG GCACGCATTG ACAGCCACGG CAGGATCTTC 
TACGTGGATC ACGTAAACAG AACCACGACG TGGCAGCGAC CGACAGCTCC CCCAGCCCCA 
CAGGTGCTGC AGAGATCTAA CTCCATACAG CAAATGGAGC AGCTGAACCG GCGATATCAG 
AGTATCCGCA GAACCATGAC TAATGAGAGG CCTGAGGAAA ATACCAATGC TATTGATGGG 
GCAGGGGAGG AAGCTGACTT TCACCAAGCC AGTGCAGATT TCCGACGGGA GAACATCCTG 
CCTCACTCTA CCTCCAGATC CAGGCTCACG TTGCTGTTAC AGTCTCCCCC CGTGAAGTTC 
CTCATCAGCC CAGAGTTCTT CACCGTGCTG CATTCTAACC CTAGTGCCTA CCGCATGTTT 
ACAAACAACA CGTGTTTGAA GCACATGATC ACCAAAGTCC GGAGGGACAC CCACCACTTT 
GAACGCTACC AGCATAACCG GGACCTTGTG GGATTCCTCA ACATGTTCGC GAACAAACAG 
CTAGAGCTGC CGCGGGGATG GGAAATGAAA CATGATCACC AGGGCAAGGC ATTCTTTGTG 
GACCACAACT CCCGCACCAC CACTTTCATT GATCCCCGGC TCCCACTTCA GAGCAGTAGA 
CCCACAAGTG CGCTGGTTCA TCGGCAACAC CTGACAAGGC AACGCAGCCA CAGTGCGGGT 
GAGGTAGGAG AAGATTCTCG ACATGCAGGA CCACCAGTTC TTCCCAGGCC ATCCAGTACA 
TTCAATACAG TCAGTAGGCC ACAGTACCAG GACATGGTTC CAGTGGCTTA CAATGACAAG 
ATTGTTGCAT TTCTACGTCA ACCCAATATC TTTGAAATTC TACAGGAGCG TCAACCTGAT 
CTTACCAGGA ATCACTCACT CAGGGAGAAG ATCCAATTTA TCCGAACTGA AGGGACTCCA 
GGATTGGTGC GCCTTTCAAG CGATGCAGAC CTTGTTATGT TGCTGAGCTT ATTTGAAGAA 
GAGATAATGT CATACGTGCC TCCACATGCC TTACTCCACC CCAGCTACTG TCAGTCCCCA 
CGTGGCTCTC CCGTGTCATC TCCTCAGAAC TCGCCAGGTA CCCAGCGTGC CAATGCCCGG 
GCTCCAGCCC CTTACAAGCG GGATTTCGAA GCCAAACTGA GGAACTTTTA CAGGAAGTTA 
GAGACTAAAG GATATGGACA AGGCCCAGGG AAGTTAAAGT TAATTATCCG AAGAGATCAC 
TTACTAGAAG ATGCTTTTAA TCAGATTATG GGCTACTCCA GAAAAGACCT GCAGAGAAAT 
AAGCTGTATG TCACCTTCGT CGGGGAGGAA GGGCTGGATT ACAGTGGGCC TTCTAGAGAG 
TTTTTCTTCC TGGTATCCAG AGAACTCTTT AACCCATATT ATGGCTTATT TGAATATTCA 
GCCAATGACA CATACACAGT ACAAATAAGT CCTATGTCTG CTTTTGTAGA CAATCACCAT 
GAATGGTTCC GATTCAGTGG TAGGATCCTT GGTCTTGCAC TAATACACCA GTATTTGTTG 
GATGCCTTCT TCACACGGCC CTTTTATAAG GCTCTTCTCA GAATTCTATG TGACCTGAGT 
GACCTAGAAT ACCTTGATGA AGAGTTCCAT CAGAGCCTGC AGTGGATGAA AGACAATGAT 
ATCCATGACA TCCTAGACCT CACGTTCACT GTGAACGAAG AAGTATTTGG GCAGATAACT 
GAACGAGAAT TAAAGCCAGG GGGTGCCAAT ATCCCAGTTA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGA GGATGGTGAA GTGGAGGATT GAGAGGGGTG TTGTACAGCA AACAGAGAGC 
TTAGTGCGTG GCTTCTATGA GGTGGTGGAT GCCAGGCTGG TATCTGTTTT TGATGCAAGA 
GAACTGGAAT TGGTCATCGC AGGCACAGCT GAAATAGACC TAAGTGATTG GAGAAACAAC 
ACAGAATATA GAGGAGGATA CCATGACAAT CATATTGTAA TTCGGTGGTT CTGGGCTGCA 
GTGGAAAGAT TCAACAATGA ACAACGACTA AGGTTGTTAC AGTTTGTTAC AGGCACATCC 
AGCATTCCCT ATGAAGGATT TGCTTCACTC CGAGGGAGTA ACGGCCCAAG AAGATTCTGT 
GTGGAGAAAT GGGGGAAAAT CACTGCTCTT CCCAGAGCGC ATACATGTTT TAACCGTCTG 
GATCTGCCTC CCTACCCATC CTTTTCCATG CTTTATGAAA AACTGTTGAC AGCAGTTGAA 
GAAACCAGTA CTTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442185.1
CDS	478..4824
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009443910.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009443910.3

ORF Insert Sequence:

ATGGAACCGG AGATAAAAAT CTGTTTTAAA TATTACCACG GCATTAGTGG AGCCCTGCGA 
GCCACAACCC CCTGCATCAC CGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAGGGAGGTG CTTCAGGAAA CCTGCATTCT CGAAAACTTG TTAGCTTTAC ATTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAAGAGAGA 
CGGTCTACTA TCATCAGTAA CACCACCAAT CCAATTTGGC ACCGAGAGAA ATATTCCTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCCATCATCA AGCGTTTTCT GGGGAAACTA ACCATTCCAG TCCAGAGGCT GCTGGAGCGA 
CAAGCCATCG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAAAGTGGAG GTTACATCTT CTGTTCATGA AGAAATGCTG 
TTTTCTCATT CAGATGCCTC TCCAGAAGCT GTTGGCACAA TACTTGGAGT CAATTCTGTG 
AATGGAGACT TAGGTAGCCC TTCCGATGAC GAGGACATGC CAGGGAGCCA TCACGACAGC 
CAGGTGTGCT CTAATGGGCC AGTTTCTGAG GACAGTGCTG CCGATGGAAC CCCCAAGCAT 
TCACTCAGGA CTAGCTCCAC TCTGGAAATA GACACAGAGG AATTAACCTC TACTTCTTCA 
AGGACCTCAC CTCCCAGAGG ACGTCAGGAT TCACTCAATG ATTACTTAGA TGCTATCGAA 
CACAATGGCC ACTCCAGGCC GGGGACAGCG ACCTGCTCTG AGAGGTCCAT GGGTGCCTCT 
CCGAAACTGA GGAGTAGTTT TCCCACTGAC ACAAGACTCA ATGCCATGCT TCATATTGAT 
TCAGATGAAG AAGATCACGA GTTCCAGCAA GACCTGGGTT ACCCATCTTC CTTGGAGGAG 
GAGGGAGGCC TGATCATGTT TAGCAGGGCA TCCAGAGCTG ATGATGGAAG CCTGACATCT 
CAGACAAAGC TGGAGGACAA TCCTGTTGAG AATGAGGAAG CCTCCATACA CGAAGCTGCT 
TCCTTTGAGG ATAAGCCAGA AAATCTTCCA GAGCTTGCAG AGAGCTCCTT ACCTGCAGGC 
CCAGCCCCAG AGGAAGGTGA AGGCGGCCCA GAGTCTCAAC CCAGTGCTGA CCAGGGCAGT 
GCTGAACTGT GTGGCTCTCA AGAGGTAGAT CAGCCCACAA GTGGGGCAGA CACGGGGACT 
TCTGATGCAT CAGGAGGAAG CCGAAGAGCC GTCAGTGAGA CCGAGTCTCT CGATCAGGGC 
TCTGAGCCTT CCCAGGTGTC CTCTGAAACA GAACCCAGTG ATCCTGCCAG GACAGAGAGC 
GTGAGCGAAG CCAGCACCAG GCCTGAGGGA GAGAGTGACC TGGAATGCGC TGACAGCTCC 
TGCAATGAGA GTGTGACCAC GCAGCTGTCC TCTGTGGACA CACGGTGCTC CTCTCTTGAG 
AGCGCACGGT TTCCTGAAAC CCCAGCCTTT TCTTCTCAGG AGGAGGAAGA CGGAGCCTGT 
GCAGCAGAGC CCACCAGCAG TGGCCCTGCC GAAGGGTCGC AGGAATCCGT GTGCACTGCT 
GGTTCTTTAC CTGTGGTACA GGTGCCCAGT GGGGAGGATG AAGGGCCAGG GGCAGAATCG 
GCCACTGTAC CTGACCAGGA GTTGCTGGGG GAGGTCTGGC AGCGGAGGGG GAGCCTGGAG 
GGAGCTGCAG CTGCTGCTGA GAGCCCACTG CAAGAAGAAG GCAGTGCTGG GGAGGCCCAA 
GGCACCTGTG AAGGGGCAAC TGCCCAGGAG GAGGGCGCTA CTGGAGGTTC TCAAGCCAAC 
GGCCACCAGC CACTGCGAAC ACTACCTTCA GTGCGCCAGG ATGTTAGCCG GTACCAGAGG 
GTGGACGAGG CTCTCCCACC AAACTGGGAG GCACGCATTG ACAGCCACGG CAGGATCTTC 
TACGTGGATC ACGTAAACAG AACCACGACG TGGCAGCGAC CGACAGCTCC CCCAGCCCCA 
CAGGTGCTGC AGAGATCTAA CTCCATACAG CAAATGGAGC AGCTGAACCG GCGATATCAG 
AGTATCCGCA GAACCATGAC TAATGAGAGG CCTGAGGAAA ATACCAATGC TATTGATGGG 
GCAGGGGAGG AAGCTGACTT TCACCAAGCC AGTGCAGATT TCCGACGGGA GAACATCCTG 
CCTCACTCTA CCTCCAGATC CAGGCTCACG TTGCTGTTAC AGTCTCCCCC CGTGAAGTTC 
CTCATCAGCC CAGAGTTCTT CACCGTGCTG CATTCTAACC CTAGTGCCTA CCGCATGTTT 
ACAAACAACA CGTGTTTGAA GCACATGATC ACCAAAGTCC GGAGGGACAC CCACCACTTT 
GAACGCTACC AGCATAACCG GGACCTTGTG GGATTCCTCA ACATGTTCGC GAACAAACAG 
CTAGAGCTGC CGCGGGGATG GGAAATGAAA CATGATCACC AGGGCAAGGC ATTCTTTGTG 
GACCACAACT CCCGCACCAC CACTTTCATT GATCCCCGGC TCCCACTTCA GAGCAGTAGA 
CCCACAAGTG CGCTGGTTCA TCGGCAACAC CTGACAAGGC AACGCAGCCA CAGTGCGGGT 
GAGGTAGGAG AAGATTCTCG ACATGCAGGA CCACCAGTTC TTCCCAGGCC ATCCAGTACA 
TTCAATACAG TCAGTAGGCC ACAGTACCAG GACATGGTTC CAGTGGCTTA CAATGACAAG 
ATTGTTGCAT TTCTACGTCA ACCCAATATC TTTGAAATTC TACAGGAGCG TCAACCTGAT 
CTTACCAGGA ATCACTCACT CAGGGAGAAG ATCCAATTTA TCCGAACTGA AGGGACTCCA 
GGATTGGTGC GCCTTTCAAG CGATGCAGAC CTTGTTATGT TGCTGAGCTT ATTTGAAGAA 
GAGATAATGT CATACGTGCC TCCACATGCC TTACTCCACC CCAGCTACTG TCAGTCCCCA 
CGTGGCTCTC CCGTGTCATC TCCTCAGAAC TCGCCAGGTA CCCAGCGTGC CAATGCCCGG 
GCTCCAGCCC CTTACAAGCG GGATTTCGAA GCCAAACTGA GGAACTTTTA CAGGAAGTTA 
GAGACTAAAG GATATGGACA AGGCCCAGGG AAGTTAAAGT TAATTATCCG AAGAGATCAC 
TTACTAGAAG ATGCTTTTAA TCAGATTATG GGCTACTCCA GAAAAGACCT GCAGAGAAAT 
AAGCTGTATG TCACCTTCGT CGGGGAGGAA GGGCTGGATT ACAGTGGGCC TTCTAGAGAG 
TTTTTCTTCC TGGTATCCAG AGAACTCTTT AACCCATATT ATGGCTTATT TGAATATTCA 
GCCAATGACA CATACACAGT ACAAATAAGT CCTATGTCTG CTTTTGTAGA CAATCACCAT 
GAATGGTTCC GATTCAGTGG TAGGATCCTT GGTCTTGCAC TAATACACCA GTATTTGTTG 
GATGCCTTCT TCACACGGCC CTTTTATAAG GCTCTTCTCA GAATTCTATG TGACCTGAGT 
GACCTAGAAT ACCTTGATGA AGAGTTCCAT CAGAGCCTGC AGTGGATGAA AGACAATGAT 
ATCCATGACA TCCTAGACCT CACGTTCACT GTGAACGAAG AAGTATTTGG GCAGATAACT 
GAACGAGAAT TAAAGCCAGG GGGTGCCAAT ATCCCAGTTA CAGAGAAGAA CAAGAAGGAG 
TACATCGAGA GGATGGTGAA GTGGAGGATT GAGAGGGGTG TTGTACAGCA AACAGAGAGC 
TTAGTGCGTG GCTTCTATGA GGTGGTGGAT GCCAGGCTGG TATCTGTTTT TGATGCAAGA 
GAACTGGAAT TGGTCATCGC AGGCACAGCT GAAATAGACC TAAGTGATTG GAGAAACAAC 
ACAGAATATA GAGGAGGATA CCATGACAAT CATATTGTAA TTCGGTGGTT CTGGGCTGCA 
GTGGAAAGAT TCAACAATGA ACAACGACTA AGGTTGTTAC AGTTTGTTAC AGGCACATCC 
AGCATTCCCT ATGAAGGATT TGCTTCACTC CGAGGGAGTA ACGGCCCAAG AAGATTCTGT 
GTGGAGAAAT GGGGGAAAAT CACTGCTCTT CCCAGAGCGC ATACATGTTT TAACCGTCTG 
GATCTGCCTC CCTACCCATC CTTTTCCATG CTTTATGAAA AACTGTTGAC AGCAGTTGAA 
GAAACCAGTA CTTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_009443908.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442183.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

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CloneID	OPc80469
Clone ID Related Accession (Same CDS sequence)	XM_009443908.2 , XM_009443907.3 , XM_009443906.2 , XM_009443907.2 , XM_009443908.3
Accession Version	XM_009443908.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009443908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442183.1
CDS	102..4841
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009443908.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009443908.2

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_009443907.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442182.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OPc80469
Clone ID Related Accession (Same CDS sequence)	XM_009443908.2 , XM_009443907.3 , XM_009443906.2 , XM_009443907.2 , XM_009443908.3
Accession Version	XM_009443907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009443907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442182.1
CDS	330..5069
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009443907.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009443907.3

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_009443906.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442181.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

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CloneID	OPc80469
Clone ID Related Accession (Same CDS sequence)	XM_009443908.2 , XM_009443907.3 , XM_009443906.2 , XM_009443907.2 , XM_009443908.3
Accession Version	XM_009443906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009443906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442181.1
CDS	598..5337
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009443906.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009443906.2

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_009443907.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442182.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OPc80469
Clone ID Related Accession (Same CDS sequence)	XM_009443908.2 , XM_009443907.3 , XM_009443906.2 , XM_009443907.2 , XM_009443908.3
Accession Version	XM_009443907.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009443907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442182.1
CDS	331..5070
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009443907.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009443907.2

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_009443908.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442183.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc80469
Clone ID Related Accession (Same CDS sequence)	XM_009443908.2 , XM_009443907.3 , XM_009443906.2 , XM_009443907.2 , XM_009443908.3
Accession Version	XM_009443908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009443908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442183.1
CDS	109..4848
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009443908.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009443908.3

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT TACAGGCACA TCCAGCATTC CCTATGAAGG ATTTGCTTCA 
CTCCGAGGGA GTAACGGCCC AAGAAGATTC TGTGTGGAGA AATGGGGGAA AATCACTGCT 
CTTCCCAGAG CGCATACATG TTTTAACCGT CTGGATCTGC CTCCCTACCC ATCCTTTTCC 
ATGCTTTATG AAAAACTGTT GACAGCAGTT GAAGAAACCA GTACTTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_516001.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_516001.2 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

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CloneID	OPc80470
Clone ID Related Accession (Same CDS sequence)	XM_516001.5 , XM_024354993.1 , XM_516001.4
Accession Version	XM_516001.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_516001.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGGTGTG CTCTAATGGG CCAGTTTCTG AGGACAGTGC TGCCGATGGA 
ACCCCCAAGC ATTCACTCAG GACTAGCTCC ACTCTGGAAA TAGACACAGA GGAATTAACC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAATGG CCACTCCAGG CCGGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGTGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACTG ACACAAGACT CAATGCCATG 
CTTCATATTG ATTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTGGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA GCTGGAGGAC AATCCTGTTG AGAATGAGGA AGCCTCCATA 
CACGAAGCTG CTTCCTTTGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAGAGCTCC 
TTACCTGCAG GCCCAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGGCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CCGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCTGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GCGTGAGCGA AGCCAGCACC AGGCCTGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CCTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA CACACGGTGC 
TCCTCTCTTG AGAGCGCACG GTTTCCTGAA ACCCCAGCCT TTTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGTGGCCCTG CCGAAGGGTC GCAGGAATCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA TGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGTTGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCT GAGAGCCCAC TGCAAGAAGA AGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA ACACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTCCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACGA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CACAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGTATCCG CAGAACCATG ACTAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATTGATG GGGCAGGGGA GGAAGCTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGAGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTC 
GCGAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCATTCTTTG TGGACCACAA CTCCCGCACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCCACAAG TGCGCTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACATGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTTGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATACGTG CCTCCACATG CCTTACTCCA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCGCCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTGTA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCTAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGACCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCA GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAG 
CAAACAGAGA GCTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTT 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CAGTGGAAAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACAGGCACAT CCAGCATTCC CTATGAAGGA TTTGCTTCAC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCATACATGT 
TTTAACCGTC TGGATCTGCC TCCCTACCCA TCCTTTTCCA TGCTTTATGA AAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_516001.2
CDS	330..5048
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_516001.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_516001.5

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGGTGTG CTCTAATGGG CCAGTTTCTG AGGACAGTGC TGCCGATGGA 
ACCCCCAAGC ATTCACTCAG GACTAGCTCC ACTCTGGAAA TAGACACAGA GGAATTAACC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAATGG CCACTCCAGG CCGGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGTGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACTG ACACAAGACT CAATGCCATG 
CTTCATATTG ATTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTGGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA GCTGGAGGAC AATCCTGTTG AGAATGAGGA AGCCTCCATA 
CACGAAGCTG CTTCCTTTGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAGAGCTCC 
TTACCTGCAG GCCCAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGGCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CCGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCTGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GCGTGAGCGA AGCCAGCACC AGGCCTGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CCTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA CACACGGTGC 
TCCTCTCTTG AGAGCGCACG GTTTCCTGAA ACCCCAGCCT TTTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGTGGCCCTG CCGAAGGGTC GCAGGAATCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA TGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGTTGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCT GAGAGCCCAC TGCAAGAAGA AGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA ACACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTCCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACGA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CACAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGTATCCG CAGAACCATG ACTAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATTGATG GGGCAGGGGA GGAAGCTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGAGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTC 
GCGAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCATTCTTTG TGGACCACAA CTCCCGCACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCCACAAG TGCGCTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACATGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTTGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATACGTG CCTCCACATG CCTTACTCCA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCGCCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTGTA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCTAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGACCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCA GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAG 
CAAACAGAGA GCTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTT 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CAGTGGAAAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACAGGCACAT CCAGCATTCC CTATGAAGGA TTTGCTTCAC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCATACATGT 
TTTAACCGTC TGGATCTGCC TCCCTACCCA TCCTTTTCCA TGCTTTATGA AAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_024354993.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024210761.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

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CloneID	OPc80470
Clone ID Related Accession (Same CDS sequence)	XM_516001.5 , XM_024354993.1 , XM_516001.4
Accession Version	XM_024354993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGGTGTG CTCTAATGGG CCAGTTTCTG AGGACAGTGC TGCCGATGGA 
ACCCCCAAGC ATTCACTCAG GACTAGCTCC ACTCTGGAAA TAGACACAGA GGAATTAACC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAATGG CCACTCCAGG CCGGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGTGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACTG ACACAAGACT CAATGCCATG 
CTTCATATTG ATTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTGGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA GCTGGAGGAC AATCCTGTTG AGAATGAGGA AGCCTCCATA 
CACGAAGCTG CTTCCTTTGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAGAGCTCC 
TTACCTGCAG GCCCAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGGCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CCGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCTGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GCGTGAGCGA AGCCAGCACC AGGCCTGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CCTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA CACACGGTGC 
TCCTCTCTTG AGAGCGCACG GTTTCCTGAA ACCCCAGCCT TTTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGTGGCCCTG CCGAAGGGTC GCAGGAATCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA TGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGTTGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCT GAGAGCCCAC TGCAAGAAGA AGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA ACACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTCCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACGA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CACAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGTATCCG CAGAACCATG ACTAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATTGATG GGGCAGGGGA GGAAGCTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGAGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTC 
GCGAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCATTCTTTG TGGACCACAA CTCCCGCACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCCACAAG TGCGCTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACATGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTTGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATACGTG CCTCCACATG CCTTACTCCA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCGCCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTGTA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCTAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGACCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCA GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAG 
CAAACAGAGA GCTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTT 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CAGTGGAAAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACAGGCACAT CCAGCATTCC CTATGAAGGA TTTGCTTCAC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCATACATGT 
TTTAACCGTC TGGATCTGCC TCCCTACCCA TCCTTTTCCA TGCTTTATGA AAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024210761.1
CDS	598..5316
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_024354993.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024354993.1

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGGTGTG CTCTAATGGG CCAGTTTCTG AGGACAGTGC TGCCGATGGA 
ACCCCCAAGC ATTCACTCAG GACTAGCTCC ACTCTGGAAA TAGACACAGA GGAATTAACC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAATGG CCACTCCAGG CCGGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGTGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACTG ACACAAGACT CAATGCCATG 
CTTCATATTG ATTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTGGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA GCTGGAGGAC AATCCTGTTG AGAATGAGGA AGCCTCCATA 
CACGAAGCTG CTTCCTTTGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAGAGCTCC 
TTACCTGCAG GCCCAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGGCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CCGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCTGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GCGTGAGCGA AGCCAGCACC AGGCCTGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CCTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA CACACGGTGC 
TCCTCTCTTG AGAGCGCACG GTTTCCTGAA ACCCCAGCCT TTTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGTGGCCCTG CCGAAGGGTC GCAGGAATCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA TGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGTTGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCT GAGAGCCCAC TGCAAGAAGA AGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA ACACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTCCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACGA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CACAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGTATCCG CAGAACCATG ACTAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATTGATG GGGCAGGGGA GGAAGCTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGAGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTC 
GCGAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCATTCTTTG TGGACCACAA CTCCCGCACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCCACAAG TGCGCTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACATGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTTGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATACGTG CCTCCACATG CCTTACTCCA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCGCCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTGTA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCTAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGACCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCA GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAG 
CAAACAGAGA GCTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTT 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CAGTGGAAAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACAGGCACAT CCAGCATTCC CTATGAAGGA TTTGCTTCAC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCATACATGT 
TTTAACCGTC TGGATCTGCC TCCCTACCCA TCCTTTTCCA TGCTTTATGA AAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_516001.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_516001.2 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

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CloneID	OPc80470
Clone ID Related Accession (Same CDS sequence)	XM_516001.5 , XM_024354993.1 , XM_516001.4
Accession Version	XM_516001.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_516001.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGGTGTG CTCTAATGGG CCAGTTTCTG AGGACAGTGC TGCCGATGGA 
ACCCCCAAGC ATTCACTCAG GACTAGCTCC ACTCTGGAAA TAGACACAGA GGAATTAACC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAATGG CCACTCCAGG CCGGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGTGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACTG ACACAAGACT CAATGCCATG 
CTTCATATTG ATTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTGGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA GCTGGAGGAC AATCCTGTTG AGAATGAGGA AGCCTCCATA 
CACGAAGCTG CTTCCTTTGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAGAGCTCC 
TTACCTGCAG GCCCAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGGCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CCGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCTGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GCGTGAGCGA AGCCAGCACC AGGCCTGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CCTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA CACACGGTGC 
TCCTCTCTTG AGAGCGCACG GTTTCCTGAA ACCCCAGCCT TTTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGTGGCCCTG CCGAAGGGTC GCAGGAATCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA TGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGTTGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCT GAGAGCCCAC TGCAAGAAGA AGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA ACACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTCCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACGA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CACAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGTATCCG CAGAACCATG ACTAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATTGATG GGGCAGGGGA GGAAGCTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGAGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTC 
GCGAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCATTCTTTG TGGACCACAA CTCCCGCACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCCACAAG TGCGCTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACATGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTTGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATACGTG CCTCCACATG CCTTACTCCA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCGCCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTGTA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCTAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGACCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCA GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAG 
CAAACAGAGA GCTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTT 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CAGTGGAAAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACAGGCACAT CCAGCATTCC CTATGAAGGA TTTGCTTCAC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCATACATGT 
TTTAACCGTC TGGATCTGCC TCCCTACCCA TCCTTTTCCA TGCTTTATGA AAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_516001.2
CDS	331..5049
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_516001.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_516001.4

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGGTGTG CTCTAATGGG CCAGTTTCTG AGGACAGTGC TGCCGATGGA 
ACCCCCAAGC ATTCACTCAG GACTAGCTCC ACTCTGGAAA TAGACACAGA GGAATTAACC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAATGG CCACTCCAGG CCGGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGTGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACTG ACACAAGACT CAATGCCATG 
CTTCATATTG ATTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTGGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA GCTGGAGGAC AATCCTGTTG AGAATGAGGA AGCCTCCATA 
CACGAAGCTG CTTCCTTTGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAGAGCTCC 
TTACCTGCAG GCCCAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGGCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CCGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCTGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GCGTGAGCGA AGCCAGCACC AGGCCTGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CCTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA CACACGGTGC 
TCCTCTCTTG AGAGCGCACG GTTTCCTGAA ACCCCAGCCT TTTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGTGGCCCTG CCGAAGGGTC GCAGGAATCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA TGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGTTGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCT GAGAGCCCAC TGCAAGAAGA AGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA ACACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTCCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACGA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CACAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGTATCCG CAGAACCATG ACTAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATTGATG GGGCAGGGGA GGAAGCTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGAGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTC 
GCGAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCATTCTTTG TGGACCACAA CTCCCGCACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCCACAAG TGCGCTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACATGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTTGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATACGTG CCTCCACATG CCTTACTCCA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCGCCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTGTA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCTAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGACCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCA GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAG 
CAAACAGAGA GCTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTT 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CAGTGGAAAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACAGGCACAT CCAGCATTCC CTATGAAGGA TTTGCTTCAC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCATACATGT 
TTTAACCGTC TGGATCTGCC TCCCTACCCA TCCTTTTCCA TGCTTTATGA AAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_016950255.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016805744.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc80471
Clone ID Related Accession (Same CDS sequence)	XM_016950255.1 , XM_016950255.2
Accession Version	XM_016950255.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGGAGCT CTGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016805744.1
CDS	333..4862
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQELCP

Target ORF information:

RefSeq Version	XM_016950255.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950255.1

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGGAGCT CTGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_016950255.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016805744.1 Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc80471
Clone ID Related Accession (Same CDS sequence)	XM_016950255.1 , XM_016950255.2
Accession Version	XM_016950255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGGAGCT CTGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016805744.1
CDS	332..4861
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASIHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPESQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQELLGEVWQRRGSLEGAAAAAESPLQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRTLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQELCP

Target ORF information:

RefSeq Version	XM_016950255.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950255.2

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTGCTT TTTGTGAGGC GTCGAAATCC CCAGATGCGG 
TACACATTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TTGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAAGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAAGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGAAATG CTGTTTTCTC ATTCAGATGC CTCTCCAGAA 
GCTGTTGGCA CAATACTTGG AGTCAATTCT GTGAATGGAG ACTTAGGTAG CCCTTCCGAT 
GACGAGGACA TGCCAGGGAG CCATCACGAC AGCCAGGTGT GCTCTAATGG GCCAGTTTCT 
GAGGACAGTG CTGCCGATGG AACCCCCAAG CATTCACTCA GGACTAGCTC CACTCTGGAA 
ATAGACACAG AGGAATTAAC CTCTACTTCT TCAAGGACCT CACCTCCCAG AGGACGTCAG 
GATTCACTCA ATGATTACTT AGATGCTATC GAACACAATG GCCACTCCAG GCCGGGGACA 
GCGACCTGCT CTGAGAGGTC CATGGGTGCC TCTCCGAAAC TGAGGAGTAG TTTTCCCACT 
GACACAAGAC TCAATGCCAT GCTTCATATT GATTCAGATG AAGAAGATCA CGAGTTCCAG 
CAAGACCTGG GTTACCCATC TTCCTTGGAG GAGGAGGGAG GCCTGATCAT GTTTAGCAGG 
GCATCCAGAG CTGATGATGG AAGCCTGACA TCTCAGACAA AGCTGGAGGA CAATCCTGTT 
GAGAATGAGG AAGCCTCCAT ACACGAAGCT GCTTCCTTTG AGGATAAGCC AGAAAATCTT 
CCAGAGCTTG CAGAGAGCTC CTTACCTGCA GGCCCAGCCC CAGAGGAAGG TGAAGGCGGC 
CCAGAGTCTC AACCCAGTGC TGACCAGGGC AGTGCTGAAC TGTGTGGCTC TCAAGAGGTA 
GATCAGCCCA CAAGTGGGGC AGACACGGGG ACTTCTGATG CATCAGGAGG AAGCCGAAGA 
GCCGTCAGTG AGACCGAGTC TCTCGATCAG GGCTCTGAGC CTTCCCAGGT GTCCTCTGAA 
ACAGAACCCA GTGATCCTGC CAGGACAGAG AGCGTGAGCG AAGCCAGCAC CAGGCCTGAG 
GGAGAGAGTG ACCTGGAATG CGCTGACAGC TCCTGCAATG AGAGTGTGAC CACGCAGCTG 
TCCTCTGTGG ACACACGGTG CTCCTCTCTT GAGAGCGCAC GGTTTCCTGA AACCCCAGCC 
TTTTCTTCTC AGGAGGAGGA AGACGGAGCC TGTGCAGCAG AGCCCACCAG CAGTGGCCCT 
GCCGAAGGGT CGCAGGAATC CGTGTGCACT GCTGGTTCTT TACCTGTGGT ACAGGTGCCC 
AGTGGGGAGG ATGAAGGGCC AGGGGCAGAA TCGGCCACTG TACCTGACCA GGAGTTGCTG 
GGGGAGGTCT GGCAGCGGAG GGGGAGCCTG GAGGGAGCTG CAGCTGCTGC TGAGAGCCCA 
CTGCAAGAAG AAGGCAGTGC TGGGGAGGCC CAAGGCACCT GTGAAGGGGC AACTGCCCAG 
GAGGAGGGCG CTACTGGAGG TTCTCAAGCC AACGGCCACC AGCCACTGCG AACACTACCT 
TCAGTGCGCC AGGATGTTAG CCGGTACCAG AGGGTGGACG AGGCTCTCCC ACCAAACTGG 
GAGGCACGCA TTGACAGCCA CGGCAGGATC TTCTACGTGG ATCACGTAAA CAGAACCACG 
ACGTGGCAGC GACCGACAGC TCCCCCAGCC CCACAGGTGC TGCAGAGATC TAACTCCATA 
CAGCAAATGG AGCAGCTGAA CCGGCGATAT CAGAGTATCC GCAGAACCAT GACTAATGAG 
AGGCCTGAGG AAAATACCAA TGCTATTGAT GGGGCAGGGG AGGAAGCTGA CTTTCACCAA 
GCCAGTGCAG ATTTCCGACG GGAGAACATC CTGCCTCACT CTACCTCCAG ATCCAGGCTC 
ACGTTGCTGT TACAGTCTCC CCCCGTGAAG TTCCTCATCA GCCCAGAGTT CTTCACCGTG 
CTGCATTCTA ACCCTAGTGC CTACCGCATG TTTACAAACA ACACGTGTTT GAAGCACATG 
ATCACCAAAG TCCGGAGGGA CACCCACCAC TTTGAACGCT ACCAGCATAA CCGGGACCTT 
GTGGGATTCC TCAACATGTT CGCGAACAAA CAGCTAGAGC TGCCGCGGGG ATGGGAAATG 
AAACATGATC ACCAGGGCAA GGCATTCTTT GTGGACCACA ACTCCCGCAC CACCACTTTC 
ATTGATCCCC GGCTCCCACT TCAGAGCAGT AGACCCACAA GTGCGCTGGT TCATCGGCAA 
CACCTGACAA GGCAACGCAG CCACAGTGCG GGTGAGGTAG GAGAAGATTC TCGACATGCA 
GGACCACCAG TTCTTCCCAG GCCATCCAGT ACATTCAATA CAGTCAGTAG GCCACAGTAC 
CAGGACATGG TTCCAGTGGC TTACAATGAC AAGATTGTTG CATTTCTACG TCAACCCAAT 
ATCTTTGAAA TTCTACAGGA GCGTCAACCT GATCTTACCA GGAATCACTC ACTCAGGGAG 
AAGATCCAAT TTATCCGAAC TGAAGGGACT CCAGGATTGG TGCGCCTTTC AAGCGATGCA 
GACCTTGTTA TGTTGCTGAG CTTATTTGAA GAAGAGATAA TGTCATACGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA CTGTCAGTCC CCACGTGGCT CTCCCGTGTC ATCTCCTCAG 
AACTCGCCAG GTACCCAGCG TGCCAATGCC CGGGCTCCAG CCCCTTACAA GCGGGATTTC 
GAAGCCAAAC TGAGGAACTT TTACAGGAAG TTAGAGACTA AAGGATATGG ACAAGGCCCA 
GGGAAGTTAA AGTTAATTAT CCGAAGAGAT CACTTACTAG AAGATGCTTT TAATCAGATT 
ATGGGCTACT CCAGAAAAGA CCTGCAGAGA AATAAGCTGT ATGTCACCTT CGTCGGGGAG 
GAAGGGCTGG ATTACAGTGG GCCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAACCCAT ATTATGGCTT ATTTGAATAT TCAGCCAATG ACACATACAC AGTACAAATA 
AGTCCTATGT CTGCTTTTGT AGACAATCAC CATGAATGGT TCCGATTCAG TGGTAGGATC 
CTTGGTCTTG CACTAATACA CCAGTATTTG TTGGATGCCT TCTTCACACG GCCCTTTTAT 
AAGGCTCTTC TCAGAATTCT ATGTGACCTG AGTGACCTAG AATACCTTGA TGAAGAGTTC 
CATCAGAGCC TGCAGTGGAT GAAAGACAAT GATATCCATG ACATCCTAGA CCTCACGTTC 
ACTGTGAACG AAGAAGTATT TGGGCAGATA ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TTACAGAGAA GAACAAGAAG GAGTACATCG AGAGGATGGT GAAGTGGAGG 
ATTGAGAGGG GTGTTGTACA GCAAACAGAG AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG 
GATGCCAGGC TGGTATCTGT TTTTGATGCA AGAGAACTGG AATTGGTCAT CGCAGGCACA 
GCTGAAATAG ACCTAAGTGA TTGGAGAAAC AACACAGAAT ATAGAGGAGG ATACCATGAC 
AATCATATTG TAATTCGGTG GTTCTGGGCT GCAGTGGAAA GATTCAACAA TGAACAACGA 
CTAAGGTTGT TACAGGAGCT CTGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HECW2
Entrez Gene ID	459844
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase HECW2
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

	LOC697155	XP_002808063.1
Mus musculus (house mouse)	Hecw2	NP_001001883.1
Gallus gallus (chicken)	HECW2	XP_421906.2
Bos taurus (cattle)	HECW2	XP_002685520.2
Rattus norvegicus (Norway rat)	Hecw2	NP_001101688.1
Pan troglodytes (chimpanzee)	HECW2	XP_516001.2
Canis lupus familiaris (dog)	HECW2	XP_005640790.1
Danio rerio (zebrafish)	hecw2	XP_003197660.2
Homo sapiens (human)	HECW2	NP_065811.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HECW2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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