hecw2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hecw2b
Entrez Gene ID	100331355
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b
Synonyms	hecw2
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b Names E3 ubiquitin-protein ligase HECW2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 nedl2b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003197612.4	XP_003197660.2	E3 ubiquitin-protein ligase HECW2
XM_003197612.5	XP_003197660.3	E3 ubiquitin-protein ligase HECW2

Pathways from BioSystems

Homologies

	LOC697155	XP_002808063.1
Mus musculus (house mouse)	Hecw2	NP_001001883.1
Gallus gallus (chicken)	HECW2	XP_421906.2
Bos taurus (cattle)	HECW2	XP_002685520.2
Rattus norvegicus (Norway rat)	Hecw2	NP_001101688.1
Pan troglodytes (chimpanzee)	HECW2	XP_516001.2
Canis lupus familiaris (dog)	HECW2	XP_005640790.1
Danio rerio (zebrafish)	hecw2	XP_003197660.2
Homo sapiens (human)	HECW2	NP_065811.1

Gene Ontology

The following hecw2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hecw2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05404	XM_003197612.4 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa147354	XM_003197612.5 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hecw2b ( XM_003197612.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197660.2 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.

Price & Availability↑

CloneID	ODa05404
Clone ID Related Accession (Same CDS sequence)	XM_003197612.4
Accession Version	XM_003197612.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase HECW2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003197612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA 
TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC 
AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG 
TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT 
GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC 
TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC 
GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC 
GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG 
GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT 
ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG 
TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC 
GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG 
AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT 
GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA 
CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA 
CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT 
GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA 
TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA 
GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT 
GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT 
ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT 
GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG 
CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG 
GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC 
AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT 
ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC 
GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGACTTGAC CCAAAGCATC 
AGCAGCTCTC AGCAGCCAGT TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA 
TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC 
ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC 
TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT 
CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG 
AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT 
CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC 
TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC 
AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA 
CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC 
AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG 
ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG 
CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC 
CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT 
GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG 
GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG 
TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA 
AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG 
CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC 
AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA 
CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC 
TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG 
AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT 
GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT 
TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC 
TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA 
GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC 
TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC 
CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT 
CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC 
CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG 
TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT 
GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT 
CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG 
TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG 
AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC 
GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG 
AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG 
CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG 
TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC 
TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG 
TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG 
ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG 
CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT 
TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG 
ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197660.2
CDS	2362..6780
Translation	MATGTTTTAREHLLVVRRRSPHMRYTLSPENLRSLSAQGGSGGGGSRGGAPEPMGLQRANSDTDLVTSDSRSSLTASTYQFTLGRGQNLVISWDIKEEVDATDWIGLYHIDETCPANVWDSKNRGVNGTQRGQIVWRLEPGPYFMEPETKICFKYYHGVSGALRATTPCITVKNPGVTVGAEGQVDGQSVTEHCRKLVSFTLSDIRAQGLKKGMFFNPDPYLKMSIQPGKRNGFPTFSHHGQERRTSIISNTTNPVWHGEKYTFVALMTDVLEIEVKDKFAKSRPIIKRFLGQLIIPVQRLLERHTAGDQHVSYTLCRRLPTEHVSGQLHFRVEITFNGHEDAMGSLLGASAMNGDPGSPSDDEDVLQPVSRMVRGPSPTGSEGSLHGDAFRPDECMMDPEEDRLLRDGAPDLAEGAPAHTHRQSSLNDYLDAIEAPKPPGDRPLGASSPKLRSSFPTDTRLNAMLHIDSDEDEEGHASRDTALTNGMPRPEGHASSERGKGEVHVKNRERLESEAASSATDTEATASVSSSGTTKSQNGGGAEGGQEGAAISGDLTQSISSSQQPVLRIQDEEGAAPQASSRTEGSSEAPAVSEASASAIYTVNEVGGATATNQPERMNSSEMTNQEEEDEEIWQRRRSLQASGNESQAEAMELGNEVRRTADEAQAALITDGETGACAQVNGHQPMRSLPSVRHDISRYQRVDEPLPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTITNERTEEQAAEMPPDDLTTSDLLHHSIPEYRRDGVVGPASSRSRLTLLLQSPSAKFLTSPDFFTVLHSNPSAYRMFTSNTCLKHMISKVRRDAHHFERYQHNRDLVAFLNMFANKQLDLPRGWEMKHDHTGKPFFVDHNCRATTFIDPRLPLQSARPSSLLAHRQHLSRQRSHSAGEVGDDPRHAGPAVLPRPSNTFTANRNQCQDLVPVAYNEKIVAFLRQPNIFDILQERQPELIRNHSLREKVQFIRNEGASGLARLSSDADLVILLSLFEEEVMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSKLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEYLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGANIPVSEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLGDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITSLPRAHTCFNRLDLPPYPSFSMLYEKMLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003197612.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197612.4

ORF Insert Sequence:

ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA 
TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC 
AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG 
TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT 
GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC 
TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC 
GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC 
GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG 
GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT 
ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG 
TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC 
GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG 
AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT 
GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA 
CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA 
CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT 
GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA 
TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA 
GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT 
GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT 
ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT 
GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG 
CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG 
GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC 
AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT 
ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC 
GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGACTTGAC CCAAAGCATC 
AGCAGCTCTC AGCAGCCAGT TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA 
TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC 
ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC 
TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT 
CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG 
AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT 
CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC 
TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC 
AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA 
CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC 
AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG 
ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG 
CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC 
CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT 
GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG 
GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG 
TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA 
AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG 
CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC 
AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA 
CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC 
TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG 
AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT 
GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT 
TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC 
TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA 
GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC 
TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC 
CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT 
CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC 
CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG 
TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT 
GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT 
CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG 
TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG 
AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC 
GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG 
AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG 
CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG 
TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC 
TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG 
TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG 
ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG 
CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT 
TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG 
ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw2b ( XM_003197612.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197660.3 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.

Price & Availability↑

CloneID	ODa147354
Clone ID Related Accession (Same CDS sequence)	XM_003197612.5
Accession Version	XM_003197612.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003197612.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA 
TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC 
AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG 
TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT 
GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC 
TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC 
GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC 
GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG 
GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT 
ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG 
TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC 
GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG 
AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT 
GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA 
CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA 
CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT 
GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA 
TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA 
GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT 
GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT 
ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT 
GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG 
CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG 
GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC 
AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT 
ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC 
GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGGCTTGAC CCAAAGCATC 
AGCAGCTTTC AGCAGCCAGC TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA 
TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC 
ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC 
TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT 
CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG 
AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT 
CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC 
TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC 
AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA 
CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC 
AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG 
ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG 
CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC 
CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT 
GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG 
GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG 
TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA 
AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG 
CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC 
AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA 
CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC 
TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG 
AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT 
GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT 
TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC 
TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA 
GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC 
TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC 
CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT 
CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC 
CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG 
TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT 
GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT 
CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG 
TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG 
AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC 
GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG 
AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG 
CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG 
TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC 
TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG 
TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG 
ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG 
CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT 
TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG 
ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197660.3
CDS	2364..6782
Translation	MATGTTTTAREHLLVVRRRSPHMRYTLSPENLRSLSAQGGSGGGGSRGGAPEPMGLQRANSDTDLVTSDSRSSLTASTYQFTLGRGQNLVISWDIKEEVDATDWIGLYHIDETCPANVWDSKNRGVNGTQRGQIVWRLEPGPYFMEPETKICFKYYHGVSGALRATTPCITVKNPGVTVGAEGQVDGQSVTEHCRKLVSFTLSDIRAQGLKKGMFFNPDPYLKMSIQPGKRNGFPTFSHHGQERRTSIISNTTNPVWHGEKYTFVALMTDVLEIEVKDKFAKSRPIIKRFLGQLIIPVQRLLERHTAGDQHVSYTLCRRLPTEHVSGQLHFRVEITFNGHEDAMGSLLGASAMNGDPGSPSDDEDVLQPVSRMVRGPSPTGSEGSLHGDAFRPDECMMDPEEDRLLRDGAPDLAEGAPAHTHRQSSLNDYLDAIEAPKPPGDRPLGASSPKLRSSFPTDTRLNAMLHIDSDEDEEGHASRDTALTNGMPRPEGHASSERGKGEVHVKNRERLESEAASSATDTEATASVSSSGTTKSQNGGGAEGGQEGAAISGGLTQSISSFQQPALRIQDEEGAAPQASSRTEGSSEAPAVSEASASAIYTVNEVGGATATNQPERMNSSEMTNQEEEDEEIWQRRRSLQASGNESQAEAMELGNEVRRTADEAQAALITDGETGACAQVNGHQPMRSLPSVRHDISRYQRVDEPLPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTITNERTEEQAAEMPPDDLTTSDLLHHSIPEYRRDGVVGPASSRSRLTLLLQSPSAKFLTSPDFFTVLHSNPSAYRMFTSNTCLKHMISKVRRDAHHFERYQHNRDLVAFLNMFANKQLDLPRGWEMKHDHTGKPFFVDHNCRATTFIDPRLPLQSARPSSLLAHRQHLSRQRSHSAGEVGDDPRHAGPAVLPRPSNTFTANRNQCQDLVPVAYNEKIVAFLRQPNIFDILQERQPELIRNHSLREKVQFIRNEGASGLARLSSDADLVILLSLFEEEVMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSKLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEYLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGANIPVSEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLGDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITSLPRAHTCFNRLDLPPYPSFSMLYEKMLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003197612.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197612.5

ORF Insert Sequence:

ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA 
TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC 
AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG 
TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT 
GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC 
TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC 
GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC 
GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG 
GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT 
ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG 
TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC 
GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG 
AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT 
GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA 
CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA 
CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT 
GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA 
TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA 
GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT 
GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT 
ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT 
GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG 
CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG 
GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC 
AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT 
ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC 
GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGGCTTGAC CCAAAGCATC 
AGCAGCTTTC AGCAGCCAGC TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA 
TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC 
ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC 
TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT 
CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG 
AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT 
CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC 
TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC 
AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA 
CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC 
AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG 
ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG 
CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC 
CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT 
GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG 
GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG 
TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA 
AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG 
CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC 
AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA 
CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC 
TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG 
AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT 
GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT 
TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC 
TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA 
GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC 
TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC 
CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT 
CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC 
CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG 
TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT 
GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT 
CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG 
TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG 
AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC 
GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG 
AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG 
CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG 
TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC 
TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG 
TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG 
ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG 
CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT 
TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG 
ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA