Ephb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ephb1
Entrez Gene ID	270190
Full Name	Eph receptor B1
Synonyms	9330129L11,AW488255,C130099E04Rik,Cek6,Elk,Elkh,Hek6,Net
General protein information	Preferred Names Eph receptor B1 Names ephrin type-B receptor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_173447.3	NP_775623.3	ephrin type-B receptor 1 isoform 1 precursor
NM_001168296.1	NP_001161768.1	ephrin type-B receptor 1 isoform 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EPHB1	XP_001151617.1
Danio rerio (zebrafish)	ephb1	XP_002660945.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000489	XP_310604.5
	EPHB1	XP_001115263.1
Bos taurus (cattle)	EPHB1	NP_001179758.2
Mus musculus (house mouse)	Ephb1	NP_775623.3
Xenopus tropicalis (tropical clawed frog)	ephb1	XP_002935121.2
Gallus gallus (chicken)	EPHB1	XP_422685.4
Drosophila melanogaster (fruit fly)	Eph	NP_726589.2
Homo sapiens (human)	EPHB1	NP_004432.1
Canis lupus familiaris (dog)	EPHB1	XP_542791.3
Rattus norvegicus (Norway rat)	Ephb1	NP_001097998.1

Gene Ontology

The following Ephb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ephb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19053	NM_173447.3 Latest version!	Mus musculus Eph receptor B1 (Ephb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$580.30 $829.00
OMu19479	NM_001168296.1 Latest version!	Mus musculus Eph receptor B1 (Ephb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ephb1 ( NM_173447.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775623.3 Mus musculus Eph receptor B1 (Ephb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19053
Clone ID Related Accession (Same CDS sequence)	NM_173447.3
Accession Version	NM_173447.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ephrin type-B receptor 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK039530.1, AK036148.1 and BC057301.1. On Nov 28, 2009 this sequence version replaced NM_173447.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057301.1, AK033966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGG ATTGCTTGCT GCTCTTCCTC CTGGCATCTG CAGTGGCCGC GATGGAAGAG 
ACATTGATGG ACACAAGGAC AGCCACTGCA GAGTTGGGAT GGACGGCCAA CCCTGCCTCT 
GGGTGGGAAG AAGTCAGTGG CTATGATGAA AACCTGAACA CCATCCGTAC TTACCAAGTG 
TGCAACGTCT TCGAACCCAA CCAGAACAAC TGGCTGCTTA CCACCTTTAT CAACAGAAGG 
GGCGCCCATC GCATCTATAC AGAGATGCGC TTCACTGTGA GGGACTGCAG CAGCCTTCCA 
AATGTCCCAG GCTCCTGCAA GGAGACCTTC AACTTGTACT ACTATGAGAC TGACTCTGTG 
ATTGCCACCA AGAAGTCAGC CTTCTGGTCT GAAGCCCCCT ACCTCAAAGT GGACACCATT 
GCTGCAGATG AGAGCTTCTC CCAGGTGGAT TTTGGGGGAA GGTTGATGAA AGTCAACACG 
GAAGTCAGGA GCTTTGGGCC TCTTACTAGG AACGGTTTTT ACCTCGCTTT CCAGGATTAT 
GGAGCCTGTA TGTCTCTCCT TTCTGTCCGT GTCTTCTTCA AAAAGTGTCC CAGCATCGTG 
CAGAATTTTG CAGTGTTCCC AGAAACCATG ACAGGAGCAG AGAGCACATC TCTGGTGATT 
GCTCGGGGCA CATGCATCCC AAATGCGGAA GAAGTGGATG TGCCCATAAA ACTCTACTGC 
AACGGAGATG GAGAGTGGAT GGTGCCCATT GGGCGCTGTA CCTGTAAGCC TGGCTATGAG 
CCTGAGAACA GCGTGGCCTG CAAGGCCTGT CCTGCGGGGA CCTTCAAGGC CAGCCAGGAA 
GCTGAAGGCT GCTCCCACTG CCCCTCCAAC AGTCGCTCCC CTTCAGAGGC GTCTCCCATC 
TGCACCTGCC GGACTGGCTA TTACCGAGCT GACTTTGATC CACCAGAGGT GGCGTGTACT 
AGTGTCCCAT CGGGTCCTCG AAATGTCATC TCCATCGTGA ATGAGACATC TATCATTCTA 
GAGTGGCACC CTCCAAGAGA GACTGGTGGG AGAGATGACG TGACGTACAA CATCATCTGC 
AAGAAGTGCC GAGCAGACCG CCGCAGCTGC TCCCGCTGCG ATGACAATGT GGAGTTTGTC 
CCCAGGCAAC TGGGCTTGAC TGAGTGTCGT GTCTCCATCA GTAGCCTATG GGCCCACACC 
CCGTACACCT TTGATATCCA GGCCATCAAT GGAGTCTCTA GCAAGAGTCC CTTTCCCCCA 
CAGCACGTCT CTGTCAACAT CACCACAAAC CAAGCTGCCC CCTCCACTGT TCCTATCATG 
CACCAGGTCA GTGCCACCAT GAGGAGCATC ACCTTGTCAT GGCCTCAGCC GGAGCAACCC 
AATGGCATAA TCCTGGACTA TGAGATCCGG TACTATGAGA AGGAACACAA TGAGTTCAAC 
TCTTCCATGG CCAGGAGCCA GACCAACACA GCACGTATCG ATGGGCTACG GCCTGGCATG 
GTATACGTGG TCCAGGTGCG AGCTCGAACC GTGGCTGGCT ATGGCAAGTT CAGTGGCAAG 
ATGTGTTTCC AGACTCTGAC AGATGATGAT TACAAGTCGG AGCTGAGAGA GCAGCTACCC 
CTGATTGCTG GCTCGGCAGC AGCTGGAGTC GTATTTGTTG TGTCTCTGGT GGCCATCTCT 
ATTGTCTGCA GCAGGAAACG AGCTTACAGC AAAGAGGCTG CGTACAGTGA TAAGCTTCAA 
CATTACAGCA CAGGCCGAGG CTCCCCAGGG ATGAAGATCT ACATTGACCC ATTCACTTAT 
GAGGACCCCA ATGAAGCCGT CCGGGAATTT GCCAAGGAGA TTGATGTATC TTTTGTGAAA 
ATTGAAGAGG TCATCGGAGC AGGGGAGTTC GGCGAGGTGT ACAAGGGCCG TTTGAAGCTG 
CCAGGCAAGA GGGAAATCTA TGTGGCCATC AAGACCCTGA AGGCTGGGTA CTCAGAGAAA 
CAGCGTCGGG ATTTTCTGAG CGAGGCGAGC ATCATGGGCC AGTTTGACCA TCCCAACATC 
ATTCGCCTGG AGGGTGTCGT CACCAAGAGC CGGCCTGTCA TGATCATTAC GGAGTTCATG 
GAGAACGGCG CTTTAGACTC TTTCCTCCGG CAAAATGATG GACAGTTCAC CGTGATCCAG 
CTTGTGGGGA TGCTGAGGGG CATCGCTGCT GGCATGAAGT ACCTATCTGA GATGAATTAT 
GTGCACCGGG ACCTGGCTGC TAGAAACATT CTGGTCAACA GCAACCTGGT GTGCAAAGTT 
TCTGACTTTG GTCTCTCTCG CTACCTCCAG GATGACACCT CAGACCCCAC CTACACCAGC 
TCCTTGGGAG GGAAGATCCC TGTGAGATGG ACAGCTCCAG AGGCCATCGC CTACCGCAAG 
TTTACGTCAG CCAGCGATGT CTGGAGCTAT GGGATTGTCA TGTGGGAAGT GATGTCATTT 
GGAGAGAGAC CTTACTGGGA TATGTCCAAT CAAGATGTCA TCAATGCCAT TGAGCAGGAT 
TACCGGCTGC CTCCTCCTAT GGACTGCCCA GCTGCCCTGC ACCAGCTCAT GCTGGACTGT 
TGGCAGAAGG ATCGCAATAG CCGGCCCCGT TTTGCAGAGA TCGTCAACAC CCTGGACAAG 
ATGATCCGGA ACCCAGCTAG TCTCAAGACT GTGGCAACCA TCACCGCTGT GCCTTCCCAA 
CCCCTGCTTG ACCGCTCTAT CCCAGACTTC ACGGCCTTTA CCACCGTGGA TGACTGGCTA 
AGTGCCATCA AAATGGTCCA GTACAGGGAC AGCTTCCTCA CCGCAGGCTT CACCTCCCTT 
CAGCTGGTCA CCCAGATGAC ATCAGAAGAC CTCCTGAGAA TAGGGGTAAC CTTGGCAGGC 
CATCAGAAGA AGATTCTGAG TAGCATTCAC TCAATGAGGG TCCAGATGAA CCAGTCACCA 
TCGGTAATGG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775623.3
CDS	355..3309
Translation	MALDCLLLFLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAAYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGVTLAGHQKKILSSIHSMRVQMNQSPSVMA

Target ORF information:

RefSeq Version	NM_173447.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eph receptor B1 (Ephb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173447.3

ORF Insert Sequence:

ATGGCCCTGG ATTGCTTGCT GCTCTTCCTC CTGGCATCTG CAGTGGCCGC GATGGAAGAG 
ACATTGATGG ACACAAGGAC AGCCACTGCA GAGTTGGGAT GGACGGCCAA CCCTGCCTCT 
GGGTGGGAAG AAGTCAGTGG CTATGATGAA AACCTGAACA CCATCCGTAC TTACCAAGTG 
TGCAACGTCT TCGAACCCAA CCAGAACAAC TGGCTGCTTA CCACCTTTAT CAACAGAAGG 
GGCGCCCATC GCATCTATAC AGAGATGCGC TTCACTGTGA GGGACTGCAG CAGCCTTCCA 
AATGTCCCAG GCTCCTGCAA GGAGACCTTC AACTTGTACT ACTATGAGAC TGACTCTGTG 
ATTGCCACCA AGAAGTCAGC CTTCTGGTCT GAAGCCCCCT ACCTCAAAGT GGACACCATT 
GCTGCAGATG AGAGCTTCTC CCAGGTGGAT TTTGGGGGAA GGTTGATGAA AGTCAACACG 
GAAGTCAGGA GCTTTGGGCC TCTTACTAGG AACGGTTTTT ACCTCGCTTT CCAGGATTAT 
GGAGCCTGTA TGTCTCTCCT TTCTGTCCGT GTCTTCTTCA AAAAGTGTCC CAGCATCGTG 
CAGAATTTTG CAGTGTTCCC AGAAACCATG ACAGGAGCAG AGAGCACATC TCTGGTGATT 
GCTCGGGGCA CATGCATCCC AAATGCGGAA GAAGTGGATG TGCCCATAAA ACTCTACTGC 
AACGGAGATG GAGAGTGGAT GGTGCCCATT GGGCGCTGTA CCTGTAAGCC TGGCTATGAG 
CCTGAGAACA GCGTGGCCTG CAAGGCCTGT CCTGCGGGGA CCTTCAAGGC CAGCCAGGAA 
GCTGAAGGCT GCTCCCACTG CCCCTCCAAC AGTCGCTCCC CTTCAGAGGC GTCTCCCATC 
TGCACCTGCC GGACTGGCTA TTACCGAGCT GACTTTGATC CACCAGAGGT GGCGTGTACT 
AGTGTCCCAT CGGGTCCTCG AAATGTCATC TCCATCGTGA ATGAGACATC TATCATTCTA 
GAGTGGCACC CTCCAAGAGA GACTGGTGGG AGAGATGACG TGACGTACAA CATCATCTGC 
AAGAAGTGCC GAGCAGACCG CCGCAGCTGC TCCCGCTGCG ATGACAATGT GGAGTTTGTC 
CCCAGGCAAC TGGGCTTGAC TGAGTGTCGT GTCTCCATCA GTAGCCTATG GGCCCACACC 
CCGTACACCT TTGATATCCA GGCCATCAAT GGAGTCTCTA GCAAGAGTCC CTTTCCCCCA 
CAGCACGTCT CTGTCAACAT CACCACAAAC CAAGCTGCCC CCTCCACTGT TCCTATCATG 
CACCAGGTCA GTGCCACCAT GAGGAGCATC ACCTTGTCAT GGCCTCAGCC GGAGCAACCC 
AATGGCATAA TCCTGGACTA TGAGATCCGG TACTATGAGA AGGAACACAA TGAGTTCAAC 
TCTTCCATGG CCAGGAGCCA GACCAACACA GCACGTATCG ATGGGCTACG GCCTGGCATG 
GTATACGTGG TCCAGGTGCG AGCTCGAACC GTGGCTGGCT ATGGCAAGTT CAGTGGCAAG 
ATGTGTTTCC AGACTCTGAC AGATGATGAT TACAAGTCGG AGCTGAGAGA GCAGCTACCC 
CTGATTGCTG GCTCGGCAGC AGCTGGAGTC GTATTTGTTG TGTCTCTGGT GGCCATCTCT 
ATTGTCTGCA GCAGGAAACG AGCTTACAGC AAAGAGGCTG CGTACAGTGA TAAGCTTCAA 
CATTACAGCA CAGGCCGAGG CTCCCCAGGG ATGAAGATCT ACATTGACCC ATTCACTTAT 
GAGGACCCCA ATGAAGCCGT CCGGGAATTT GCCAAGGAGA TTGATGTATC TTTTGTGAAA 
ATTGAAGAGG TCATCGGAGC AGGGGAGTTC GGCGAGGTGT ACAAGGGCCG TTTGAAGCTG 
CCAGGCAAGA GGGAAATCTA TGTGGCCATC AAGACCCTGA AGGCTGGGTA CTCAGAGAAA 
CAGCGTCGGG ATTTTCTGAG CGAGGCGAGC ATCATGGGCC AGTTTGACCA TCCCAACATC 
ATTCGCCTGG AGGGTGTCGT CACCAAGAGC CGGCCTGTCA TGATCATTAC GGAGTTCATG 
GAGAACGGCG CTTTAGACTC TTTCCTCCGG CAAAATGATG GACAGTTCAC CGTGATCCAG 
CTTGTGGGGA TGCTGAGGGG CATCGCTGCT GGCATGAAGT ACCTATCTGA GATGAATTAT 
GTGCACCGGG ACCTGGCTGC TAGAAACATT CTGGTCAACA GCAACCTGGT GTGCAAAGTT 
TCTGACTTTG GTCTCTCTCG CTACCTCCAG GATGACACCT CAGACCCCAC CTACACCAGC 
TCCTTGGGAG GGAAGATCCC TGTGAGATGG ACAGCTCCAG AGGCCATCGC CTACCGCAAG 
TTTACGTCAG CCAGCGATGT CTGGAGCTAT GGGATTGTCA TGTGGGAAGT GATGTCATTT 
GGAGAGAGAC CTTACTGGGA TATGTCCAAT CAAGATGTCA TCAATGCCAT TGAGCAGGAT 
TACCGGCTGC CTCCTCCTAT GGACTGCCCA GCTGCCCTGC ACCAGCTCAT GCTGGACTGT 
TGGCAGAAGG ATCGCAATAG CCGGCCCCGT TTTGCAGAGA TCGTCAACAC CCTGGACAAG 
ATGATCCGGA ACCCAGCTAG TCTCAAGACT GTGGCAACCA TCACCGCTGT GCCTTCCCAA 
CCCCTGCTTG ACCGCTCTAT CCCAGACTTC ACGGCCTTTA CCACCGTGGA TGACTGGCTA 
AGTGCCATCA AAATGGTCCA GTACAGGGAC AGCTTCCTCA CCGCAGGCTT CACCTCCCTT 
CAGCTGGTCA CCCAGATGAC ATCAGAAGAC CTCCTGAGAA TAGGGGTAAC CTTGGCAGGC 
CATCAGAAGA AGATTCTGAG TAGCATTCAC TCAATGAGGG TCCAGATGAA CCAGTCACCA 
TCGGTAATGG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ephb1 ( NM_001168296.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161768.1 Mus musculus Eph receptor B1 (Ephb1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19479
Clone ID Related Accession (Same CDS sequence)	NM_001168296.1
Accession Version	NM_001168296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ephrin type-B receptor 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK039530.1, AK036211.1, CT025594.13 and BC057301.1. Transcript Variant: This variant (2) lacks a coding exon compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGG ATTGCTTGCT GCTCTTCCTC CTGGCATCTG CAGTGGCCGC GATGGAAGAG 
ACATTGATGG ACACAAGGAC AGCCACTGCA GAGTTGGGAT GGACGGCCAA CCCTGCCTCT 
GGGTGGGAAG AAGTCAGTGG CTATGATGAA AACCTGAACA CCATCCGTAC TTACCAAGTG 
TGCAACGTCT TCGAACCCAA CCAGAACAAC TGGCTGCTTA CCACCTTTAT CAACAGAAGG 
GGCGCCCATC GCATCTATAC AGAGATGCGC TTCACTGTGA GGGACTGCAG CAGCCTTCCA 
AATGTCCCAG GCTCCTGCAA GGAGACCTTC AACTTGTACT ACTATGAGAC TGACTCTGTG 
ATTGCCACCA AGAAGTCAGC CTTCTGGTCT GAAGCCCCCT ACCTCAAAGT GGACACCATT 
GCTGCAGATG AGAGCTTCTC CCAGGTGGAT TTTGGGGGAA GGTTGATGAA AGTCAACACG 
GAAGTCAGGA GCTTTGGGCC TCTTACTAGG AACGGTTTTT ACCTCGCTTT CCAGGATTAT 
GGAGCCTGTA TGTCTCTCCT TTCTGTCCGT GTCTTCTTCA AAAAGTGTCC CAGCATCGTG 
CAGAATTTTG CAGTGTTCCC AGAAACCATG ACAGGAGCAG AGAGCACATC TCTGGTGATT 
GCTCGGGGCA CATGCATCCC AAATGCGGAA GAAGTGGATG TGCCCATAAA ACTCTACTGC 
AACGGAGATG GAGAGTGGAT GGTGCCCATT GGGCGCTGTA CCTGTAAGCC TGGCTATGAG 
CCTGAGAACA GCGTGGCCTG CAAGGCCTGT CCTGCGGGGA CCTTCAAGGC CAGCCAGGAA 
GCTGAAGGCT GCTCCCACTG CCCCTCCAAC AGTCGCTCCC CTTCAGAGGC GTCTCCCATC 
TGCACCTGCC GGACTGGCTA TTACCGAGCT GACTTTGATC CACCAGAGGT GGCGTGTACT 
AGTGTCCCAT CGGGTCCTCG AAATGTCATC TCCATCGTGA ATGAGACATC TATCATTCTA 
GAGTGGCACC CTCCAAGAGA GACTGGTGGG AGAGATGACG TGACGTACAA CATCATCTGC 
AAGAAGTGCC GAGCAGACCG CCGCAGCTGC TCCCGCTGCG ATGACAATGT GGAGTTTGTC 
CCCAGGCAAC TGGGCTTGAC TGAGTGTCGT GTCTCCATCA GTAGCCTATG GGCCCACACC 
CCGTACACCT TTGATATCCA GGCCATCAAT GGAGTCTCTA GCAAGAGTCC CTTTCCCCCA 
CAGCACGTCT CTGTCAACAT CACCACAAAC CAAGCTGCCC CCTCCACTGT TCCTATCATG 
CACCAGGTCA GTGCCACCAT GAGGAGCATC ACCTTGTCAT GGCCTCAGCC GGAGCAACCC 
AATGGCATAA TCCTGGACTA TGAGATCCGG TACTATGAGA AGGAACACAA TGAGTTCAAC 
TCTTCCATGG CCAGGAGCCA GACCAACACA GCACGTATCG ATGGGCTACG GCCTGGCATG 
GTATACGTGG TCCAGGTGCG AGCTCGAACC GTGGCTGGCT ATGGCAAGTT CAGTGGCAAG 
ATGTGTTTCC AGACTCTGAC AGATGATGAT TACAAGTCGG AGCTGAGAGA GCAGCTACCC 
CTGATTGCTG GCTCGGCAGC AGCTGGAGTC GTATTTGTTG TGTCTCTGGT GGCCATCTCT 
ATTGTCTGCA GCAGGAAACG AGCTTACAGC AAAGAGGCTG CGTACAGTGA TAAGCTTCAA 
CATTACAGCA CAGGCCGAGG GGAGTTCGGC GAGGTGTACA AGGGCCGTTT GAAGCTGCCA 
GGCAAGAGGG AAATCTATGT GGCCATCAAG ACCCTGAAGG CTGGGTACTC AGAGAAACAG 
CGTCGGGATT TTCTGAGCGA GGCGAGCATC ATGGGCCAGT TTGACCATCC CAACATCATT 
CGCCTGGAGG GTGTCGTCAC CAAGAGCCGG CCTGTCATGA TCATTACGGA GTTCATGGAG 
AACGGCGCTT TAGACTCTTT CCTCCGGCAA AATGATGGAC AGTTCACCGT GATCCAGCTT 
GTGGGGATGC TGAGGGGCAT CGCTGCTGGC ATGAAGTACC TATCTGAGAT GAATTATGTG 
CACCGGGACC TGGCTGCTAG AAACATTCTG GTCAACAGCA ACCTGGTGTG CAAAGTTTCT 
GACTTTGGTC TCTCTCGCTA CCTCCAGGAT GACACCTCAG ACCCCACCTA CACCAGCTCC 
TTGGGAGGGA AGATCCCTGT GAGATGGACA GCTCCAGAGG CCATCGCCTA CCGCAAGTTT 
ACGTCAGCCA GCGATGTCTG GAGCTATGGG ATTGTCATGT GGGAAGTGAT GTCATTTGGA 
GAGAGACCTT ACTGGGATAT GTCCAATCAA GATGTCATCA ATGCCATTGA GCAGGATTAC 
CGGCTGCCTC CTCCTATGGA CTGCCCAGCT GCCCTGCACC AGCTCATGCT GGACTGTTGG 
CAGAAGGATC GCAATAGCCG GCCCCGTTTT GCAGAGATCG TCAACACCCT GGACAAGATG 
ATCCGGAACC CAGCTAGTCT CAAGACTGTG GCAACCATCA CCGCTGTGCC TTCCCAACCC 
CTGCTTGACC GCTCTATCCC AGACTTCACG GCCTTTACCA CCGTGGATGA CTGGCTAAGT 
GCCATCAAAA TGGTCCAGTA CAGGGACAGC TTCCTCACCG CAGGCTTCAC CTCCCTTCAG 
CTGGTCACCC AGATGACATC AGAAGACCTC CTGAGAATAG GGGTAACCTT GGCAGGCCAT 
CAGAAGAAGA TTCTGAGTAG CATTCACTCA ATGAGGGTCC AGATGAACCA GTCACCATCG 
GTAATGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161768.1
CDS	355..3186
Translation	MALDCLLLFLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAAYSDKLQHYSTGRGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGVTLAGHQKKILSSIHSMRVQMNQSPSVMA

Target ORF information:

RefSeq Version	NM_001168296.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eph receptor B1 (Ephb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168296.1

ORF Insert Sequence:

ATGGCCCTGG ATTGCTTGCT GCTCTTCCTC CTGGCATCTG CAGTGGCCGC GATGGAAGAG 
ACATTGATGG ACACAAGGAC AGCCACTGCA GAGTTGGGAT GGACGGCCAA CCCTGCCTCT 
GGGTGGGAAG AAGTCAGTGG CTATGATGAA AACCTGAACA CCATCCGTAC TTACCAAGTG 
TGCAACGTCT TCGAACCCAA CCAGAACAAC TGGCTGCTTA CCACCTTTAT CAACAGAAGG 
GGCGCCCATC GCATCTATAC AGAGATGCGC TTCACTGTGA GGGACTGCAG CAGCCTTCCA 
AATGTCCCAG GCTCCTGCAA GGAGACCTTC AACTTGTACT ACTATGAGAC TGACTCTGTG 
ATTGCCACCA AGAAGTCAGC CTTCTGGTCT GAAGCCCCCT ACCTCAAAGT GGACACCATT 
GCTGCAGATG AGAGCTTCTC CCAGGTGGAT TTTGGGGGAA GGTTGATGAA AGTCAACACG 
GAAGTCAGGA GCTTTGGGCC TCTTACTAGG AACGGTTTTT ACCTCGCTTT CCAGGATTAT 
GGAGCCTGTA TGTCTCTCCT TTCTGTCCGT GTCTTCTTCA AAAAGTGTCC CAGCATCGTG 
CAGAATTTTG CAGTGTTCCC AGAAACCATG ACAGGAGCAG AGAGCACATC TCTGGTGATT 
GCTCGGGGCA CATGCATCCC AAATGCGGAA GAAGTGGATG TGCCCATAAA ACTCTACTGC 
AACGGAGATG GAGAGTGGAT GGTGCCCATT GGGCGCTGTA CCTGTAAGCC TGGCTATGAG 
CCTGAGAACA GCGTGGCCTG CAAGGCCTGT CCTGCGGGGA CCTTCAAGGC CAGCCAGGAA 
GCTGAAGGCT GCTCCCACTG CCCCTCCAAC AGTCGCTCCC CTTCAGAGGC GTCTCCCATC 
TGCACCTGCC GGACTGGCTA TTACCGAGCT GACTTTGATC CACCAGAGGT GGCGTGTACT 
AGTGTCCCAT CGGGTCCTCG AAATGTCATC TCCATCGTGA ATGAGACATC TATCATTCTA 
GAGTGGCACC CTCCAAGAGA GACTGGTGGG AGAGATGACG TGACGTACAA CATCATCTGC 
AAGAAGTGCC GAGCAGACCG CCGCAGCTGC TCCCGCTGCG ATGACAATGT GGAGTTTGTC 
CCCAGGCAAC TGGGCTTGAC TGAGTGTCGT GTCTCCATCA GTAGCCTATG GGCCCACACC 
CCGTACACCT TTGATATCCA GGCCATCAAT GGAGTCTCTA GCAAGAGTCC CTTTCCCCCA 
CAGCACGTCT CTGTCAACAT CACCACAAAC CAAGCTGCCC CCTCCACTGT TCCTATCATG 
CACCAGGTCA GTGCCACCAT GAGGAGCATC ACCTTGTCAT GGCCTCAGCC GGAGCAACCC 
AATGGCATAA TCCTGGACTA TGAGATCCGG TACTATGAGA AGGAACACAA TGAGTTCAAC 
TCTTCCATGG CCAGGAGCCA GACCAACACA GCACGTATCG ATGGGCTACG GCCTGGCATG 
GTATACGTGG TCCAGGTGCG AGCTCGAACC GTGGCTGGCT ATGGCAAGTT CAGTGGCAAG 
ATGTGTTTCC AGACTCTGAC AGATGATGAT TACAAGTCGG AGCTGAGAGA GCAGCTACCC 
CTGATTGCTG GCTCGGCAGC AGCTGGAGTC GTATTTGTTG TGTCTCTGGT GGCCATCTCT 
ATTGTCTGCA GCAGGAAACG AGCTTACAGC AAAGAGGCTG CGTACAGTGA TAAGCTTCAA 
CATTACAGCA CAGGCCGAGG GGAGTTCGGC GAGGTGTACA AGGGCCGTTT GAAGCTGCCA 
GGCAAGAGGG AAATCTATGT GGCCATCAAG ACCCTGAAGG CTGGGTACTC AGAGAAACAG 
CGTCGGGATT TTCTGAGCGA GGCGAGCATC ATGGGCCAGT TTGACCATCC CAACATCATT 
CGCCTGGAGG GTGTCGTCAC CAAGAGCCGG CCTGTCATGA TCATTACGGA GTTCATGGAG 
AACGGCGCTT TAGACTCTTT CCTCCGGCAA AATGATGGAC AGTTCACCGT GATCCAGCTT 
GTGGGGATGC TGAGGGGCAT CGCTGCTGGC ATGAAGTACC TATCTGAGAT GAATTATGTG 
CACCGGGACC TGGCTGCTAG AAACATTCTG GTCAACAGCA ACCTGGTGTG CAAAGTTTCT 
GACTTTGGTC TCTCTCGCTA CCTCCAGGAT GACACCTCAG ACCCCACCTA CACCAGCTCC 
TTGGGAGGGA AGATCCCTGT GAGATGGACA GCTCCAGAGG CCATCGCCTA CCGCAAGTTT 
ACGTCAGCCA GCGATGTCTG GAGCTATGGG ATTGTCATGT GGGAAGTGAT GTCATTTGGA 
GAGAGACCTT ACTGGGATAT GTCCAATCAA GATGTCATCA ATGCCATTGA GCAGGATTAC 
CGGCTGCCTC CTCCTATGGA CTGCCCAGCT GCCCTGCACC AGCTCATGCT GGACTGTTGG 
CAGAAGGATC GCAATAGCCG GCCCCGTTTT GCAGAGATCG TCAACACCCT GGACAAGATG 
ATCCGGAACC CAGCTAGTCT CAAGACTGTG GCAACCATCA CCGCTGTGCC TTCCCAACCC 
CTGCTTGACC GCTCTATCCC AGACTTCACG GCCTTTACCA CCGTGGATGA CTGGCTAAGT 
GCCATCAAAA TGGTCCAGTA CAGGGACAGC TTCCTCACCG CAGGCTTCAC CTCCCTTCAG 
CTGGTCACCC AGATGACATC AGAAGACCTC CTGAGAATAG GGGTAACCTT GGCAGGCCAT 
CAGAAGAAGA TTCTGAGTAG CATTCACTCA ATGAGGGTCC AGATGAACCA GTCACCATCG 
GTAATGGCGT GA