Eph cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eph
Entrez Gene ID	43803
Full Name	Eph receptor tyrosine kinase
Synonyms	CG1511,CT3831,DEK,Dek,Dek7,Deph,Dmel\CG1511,Ek7,RPTK Dek7,dek,eph,lincRNA.923
General protein information	Preferred Names Eph receptor tyrosine kinase Names CG1511 gene product from transcript CG1511-RE CG1511-PA CG1511-PB CG1511-PC CG1511-PD CG1511-PE CG1511-PF Eph kinase Eph receptor Eph-PA Eph-PB Eph-PC Eph-PD Eph-PE Eph-PF drosophila eph ephrin receptors
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102D1-102D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_166775.4	NP_726589.2	Eph receptor tyrosine kinase, isoform A
NM_166774.3	NP_726588.2	Eph receptor tyrosine kinase, isoform D
NM_166776.4	NP_726590.2	Eph receptor tyrosine kinase, isoform E
NM_166777.4	NP_726591.2	Eph receptor tyrosine kinase, isoform B
NM_079886.5	NP_524625.3	Eph receptor tyrosine kinase, isoform C
NM_001169353.2	NP_001162824.1	Eph receptor tyrosine kinase, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EPHB1	XP_001151617.1
Danio rerio (zebrafish)	ephb1	XP_002660945.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000489	XP_310604.5
	EPHB1	XP_001115263.1
Bos taurus (cattle)	EPHB1	NP_001179758.2
Mus musculus (house mouse)	Ephb1	NP_775623.3
Xenopus tropicalis (tropical clawed frog)	ephb1	XP_002935121.2
Gallus gallus (chicken)	EPHB1	XP_422685.4
Drosophila melanogaster (fruit fly)	Eph	NP_726589.2
Homo sapiens (human)	EPHB1	NP_004432.1
Canis lupus familiaris (dog)	EPHB1	XP_542791.3
Rattus norvegicus (Norway rat)	Ephb1	NP_001097998.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eph and Ephrin, the well-known axon guidance molecules, instruct the dendrodendritic segregation during the discrete olfactory map formation
Title: Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in

Eph receptors have important functions in shaping compartment boundaries in both vertebrate and insect development.
Title: An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.

we propose that Ephexin/Cdc42 couples synaptic Eph signaling to the modulation of presynaptic CaV2.1 channels
Title: A presynaptic homeostatic signaling system composed of the Eph receptor, ephexin, Cdc42, and CaV2.1 calcium channels.

Eph receptor guides specific axon branches of mushroom body neurons.
Title: Drosophila Eph receptor guides specific axon branches of mushroom body neurons.

role for Drosophila Eph receptor tyrosine kinase (EPH) signaling in the control of photoreceptor axon and cortical axon topography in the developing visual system
Title: Eph receptor tyrosine kinase-mediated formation of a topographic map in the Drosophila visual system.

The following Eph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00173	NM_001169353.2 Latest version!	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00276	NM_166776.4 Latest version!	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00276	NM_166775.4 Latest version!	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00277	NM_079886.5 Latest version!	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00278	NM_166777.4 Latest version!	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00279	NM_166774.3 Latest version!	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eph ( NM_001169353.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162824.1 Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa00173
Clone ID Related Accession (Same CDS sequence)	NM_001169353.2
Accession Version	NM_001169353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Eph receptor tyrosine kinase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001169353.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAAAA GCGCCCTCAA GTCTGCGTTT TACAAAAATC AAGCTCCAAT TAGCGAGCAA 
AACAGATCTC AAATTGTTTC GAGAATGTCA TTATTAAGGA CAATACTGCT TATTATAATT 
TCAATTCACT TTAAATTGGC ACATGCAGAT CAGGTTGTTC TATTGGATAC AACTCGAGAA 
GCTACATTAG AGTGGACCAG GTATCCATAT GGACCTCAAG CCCAAACACC AGGGTGGGTG 
GAGGAGTCAT TTACCGACTT CGTTAAGGGC ATTAACTGGA GGAGCTATGT GGTTTGCGAT 
GTGGCATATC ACAACGTCAA TAACTGGTTG TGGTCGCCGT TTATCGATCG TGGATCAGCA 
AACCGACTTT ACATTGAAAT TCAGTTCACA ATACGCGATT GTTCTTTATT CCCAGGAAAC 
GCCTTGTCAT GCAAGGAAAC ATTTAGCTTA CTTTTTTACG AGTTTGATGC GGCTACTAGA 
GAACCTCCAC CATGGCAAAC AGACAGCTAC AGGCTGATTG CTCGCATCGC GGCTGGCGAG 
GGGCGCTTCA ATCAGAACTC CGATGTAGAT ATTAACACAG AGGTGAAGAG TATAGCCGTC 
AACAAAAAAG GCGTATATTT TGCTTTCAGA GATCAGGGCG CTTGTATTAG CGTTCTGGCA 
GTAAAAGTAT ATTATATTAC GTGTCCTGCT GTTACCGAAA ACTTTGCACA CTTTAACGAA 
ACACCTACGG GAAGAGAAAT TACTATAATT GAGAAACAAA ATGGAACATG TGTCGATAAC 
GCAGAACCGT ATGAGACGCC AACTTACTTA TGTAAAGGTG ATGGAAAATG GACAATTCTT 
ACTGGTGGGT GTCGTTGCAA GGCTGGCTAT GAGCCGAATT ACACAAATAA GACTTGTACA 
GAATGTCCAC TTGGGACATT TAAATCCCCA GAGGTTACAA AATGTACTCC GTGTCCTCCC 
AATTCAAATG CTTCAAAAAC TGGTTCACCT TTCTGTAAGT GTGCGTCTGG ATTCTATAGA 
CATCCAAATG ATGGTCGACA TATGCCGTGT TATAGTCCAC CAGCTGCGCC TACTAATCTC 
ACGTTGTTGT TTGTTGATCA AACAAGTGCA ATAATATCTT GGAGCGCACC GGCCAAAAAT 
GAATCGTTTT CATCTGAAAC TAATTCTAAA ATATATCACA GTGATATTGT GTACAAGATC 
AAGTGTAATA TATGCAGCCC AAATGTGGTT TACAATCCCT CAACTGACAC ATTTAACGAA 
ACAAAAATAA CACTTACAAA TCTGGAACCG GTAACGACAT ACACTGTGCA GATACACGCA 
ATAAATAGCG TGTCTCATAT TAACGAATTT AAAAGGCACT CCAACGAGTC TTCCCTGGTG 
GCAGTCAGTG ACATAGTATT TTCAAATACG TCTTTATTAA ATATACCTTT GGATTTAAAC 
GAAGTAAAGA CTGGTCAAGC TGAAATCGTT TTTACAACTG AGTCAGTTTT GCTCTCGACA 
GTTTTTAATT TGCGTATATT GGCTATAACT AATAAAGATG CCGATCTAGA ATGGGATAAA 
CCTGTTCAAA GTGATTTTCC ACTTGAATTT TATGAAGTGC GCTGGTTTCC AAAGGTCGAA 
TTAGATGCTA TAAATAAATC AGCTTTGAAC ACAAAAGAAA CGAAGGCACA TATAGTAGGA 
CTTCTAGAAA ATACGGAGTA TGGATTTCAA GTTCGTTGTA AAACGAATAA TGGATTTGGC 
TCTTATAGTA ACATGATTTA CGCCCAAACT CTTCAATCCG TTGGTTCAGT ATATGACGAT 
TCTGTGCAAA TACGATTTAT TGCTGGAGCC ATCGTGACGG GAGTATTATT TCTAGTCATT 
TTTATAATAG CTACAGTATA TTTCATGAGA TCGAAGCATC AGGATGATCT TGATAAAAAA 
TCGACAAATC ATTTGCCATT ACCACTTGAC TACGCCAGCA ACGAAGTTAA TTCTATGGAT 
ACTACTCCAA TTGTTAAAAA ACTTCATTTA AACGTGACGA CGCCTTTATT TGGAAATAGC 
AGAAGCTACG TAGATCCGCA TACCTATGAA GATCCTAATC AAGCTATAAG GGAATTCGCT 
CGAGAAATCG ATGCAAACTA CATTACAATA GAAGCTATTA TCGGAGGTGG AGAATTTGGC 
GATGTATGTC GTGGTCGATT AAAAATACCC CCAAACTTTG TACAGGACAT TGATGTGGCA 
ATAAAGACTT TAAAACCAGG GTCTTCGGAA AAAGCGCGCT GTGATTTCTT GACAGAGGCT 
TCTATTATGG GACAGTTTGA TCATCCAAAC GTTATTTATC TACAGGGAGT AGTTACTCGG 
TCTAATCCAG TTATGATTAT TACTGAGTAC ATGGAAAATG GCAGCTTAGA CACATTTCTT 
CGGGTGAATG ATGGAAAGTT TCAAACATTA CAGCTTATAG TTATGCTTCG GGGTATAGCC 
AGCGGGATGT CATATTTAAG TGATATGAAT TATGTACACA GAGACTTGGC GGCACGTAAT 
GTTCTTGTAA ATGCCCAATT AATATGCAAA ATCGCGGACT TCGGATTGTC ACGTGAAATT 
GAAAATGCAA GTGATGCATA CACAACTCGG GGCGGTAAAA TTCCTGTACG TTGGACTGCG 
CCGGAAGCCA TTGCTTTTAG AAAATTTACT TCAGCATCGG ATGTTTGGTC GTATGGAGTA 
GTTCTATGGG AGGTCATGTC CTATGGGGAA CGTCCATATT GGAACTGGTC AAATCAGGAC 
GTGATAAAGA GTATAGAAAA GGGCTACCGT TTACCAGCTC CGATGGATTG TCCAGAAGCC 
CTTTATCAGC TAATGTTGGA TTGCTGGCAA AAGCAACGTA CTCATCGCCC CACATTTGCA 
AGTATCGTTT CAACTCTTGA CAATTTGGCA CGACAACCAC AGTCATTATT GACAACACGA 
CCCTCGCCAG AATCAGACGG TAATCATATT TTGGATGGAC AGCGCGGTCA AAATATTTTC 
ATAAGCACTG ACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162824.1
CDS	357..3374
Translation	MTKSALKSAFYKNQAPISEQNRSQIVSRMSLLRTILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIAVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENASDAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNIFISTDL

Target ORF information:

RefSeq Version	NM_001169353.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169353.2

ORF Insert Sequence:

ATGACAAAAA GCGCCCTCAA GTCTGCGTTT TACAAAAATC AAGCTCCAAT TAGCGAGCAA 
AACAGATCTC AAATTGTTTC GAGAATGTCA TTATTAAGGA CAATACTGCT TATTATAATT 
TCAATTCACT TTAAATTGGC ACATGCAGAT CAGGTTGTTC TATTGGATAC AACTCGAGAA 
GCTACATTAG AGTGGACCAG GTATCCATAT GGACCTCAAG CCCAAACACC AGGGTGGGTG 
GAGGAGTCAT TTACCGACTT CGTTAAGGGC ATTAACTGGA GGAGCTATGT GGTTTGCGAT 
GTGGCATATC ACAACGTCAA TAACTGGTTG TGGTCGCCGT TTATCGATCG TGGATCAGCA 
AACCGACTTT ACATTGAAAT TCAGTTCACA ATACGCGATT GTTCTTTATT CCCAGGAAAC 
GCCTTGTCAT GCAAGGAAAC ATTTAGCTTA CTTTTTTACG AGTTTGATGC GGCTACTAGA 
GAACCTCCAC CATGGCAAAC AGACAGCTAC AGGCTGATTG CTCGCATCGC GGCTGGCGAG 
GGGCGCTTCA ATCAGAACTC CGATGTAGAT ATTAACACAG AGGTGAAGAG TATAGCCGTC 
AACAAAAAAG GCGTATATTT TGCTTTCAGA GATCAGGGCG CTTGTATTAG CGTTCTGGCA 
GTAAAAGTAT ATTATATTAC GTGTCCTGCT GTTACCGAAA ACTTTGCACA CTTTAACGAA 
ACACCTACGG GAAGAGAAAT TACTATAATT GAGAAACAAA ATGGAACATG TGTCGATAAC 
GCAGAACCGT ATGAGACGCC AACTTACTTA TGTAAAGGTG ATGGAAAATG GACAATTCTT 
ACTGGTGGGT GTCGTTGCAA GGCTGGCTAT GAGCCGAATT ACACAAATAA GACTTGTACA 
GAATGTCCAC TTGGGACATT TAAATCCCCA GAGGTTACAA AATGTACTCC GTGTCCTCCC 
AATTCAAATG CTTCAAAAAC TGGTTCACCT TTCTGTAAGT GTGCGTCTGG ATTCTATAGA 
CATCCAAATG ATGGTCGACA TATGCCGTGT TATAGTCCAC CAGCTGCGCC TACTAATCTC 
ACGTTGTTGT TTGTTGATCA AACAAGTGCA ATAATATCTT GGAGCGCACC GGCCAAAAAT 
GAATCGTTTT CATCTGAAAC TAATTCTAAA ATATATCACA GTGATATTGT GTACAAGATC 
AAGTGTAATA TATGCAGCCC AAATGTGGTT TACAATCCCT CAACTGACAC ATTTAACGAA 
ACAAAAATAA CACTTACAAA TCTGGAACCG GTAACGACAT ACACTGTGCA GATACACGCA 
ATAAATAGCG TGTCTCATAT TAACGAATTT AAAAGGCACT CCAACGAGTC TTCCCTGGTG 
GCAGTCAGTG ACATAGTATT TTCAAATACG TCTTTATTAA ATATACCTTT GGATTTAAAC 
GAAGTAAAGA CTGGTCAAGC TGAAATCGTT TTTACAACTG AGTCAGTTTT GCTCTCGACA 
GTTTTTAATT TGCGTATATT GGCTATAACT AATAAAGATG CCGATCTAGA ATGGGATAAA 
CCTGTTCAAA GTGATTTTCC ACTTGAATTT TATGAAGTGC GCTGGTTTCC AAAGGTCGAA 
TTAGATGCTA TAAATAAATC AGCTTTGAAC ACAAAAGAAA CGAAGGCACA TATAGTAGGA 
CTTCTAGAAA ATACGGAGTA TGGATTTCAA GTTCGTTGTA AAACGAATAA TGGATTTGGC 
TCTTATAGTA ACATGATTTA CGCCCAAACT CTTCAATCCG TTGGTTCAGT ATATGACGAT 
TCTGTGCAAA TACGATTTAT TGCTGGAGCC ATCGTGACGG GAGTATTATT TCTAGTCATT 
TTTATAATAG CTACAGTATA TTTCATGAGA TCGAAGCATC AGGATGATCT TGATAAAAAA 
TCGACAAATC ATTTGCCATT ACCACTTGAC TACGCCAGCA ACGAAGTTAA TTCTATGGAT 
ACTACTCCAA TTGTTAAAAA ACTTCATTTA AACGTGACGA CGCCTTTATT TGGAAATAGC 
AGAAGCTACG TAGATCCGCA TACCTATGAA GATCCTAATC AAGCTATAAG GGAATTCGCT 
CGAGAAATCG ATGCAAACTA CATTACAATA GAAGCTATTA TCGGAGGTGG AGAATTTGGC 
GATGTATGTC GTGGTCGATT AAAAATACCC CCAAACTTTG TACAGGACAT TGATGTGGCA 
ATAAAGACTT TAAAACCAGG GTCTTCGGAA AAAGCGCGCT GTGATTTCTT GACAGAGGCT 
TCTATTATGG GACAGTTTGA TCATCCAAAC GTTATTTATC TACAGGGAGT AGTTACTCGG 
TCTAATCCAG TTATGATTAT TACTGAGTAC ATGGAAAATG GCAGCTTAGA CACATTTCTT 
CGGGTGAATG ATGGAAAGTT TCAAACATTA CAGCTTATAG TTATGCTTCG GGGTATAGCC 
AGCGGGATGT CATATTTAAG TGATATGAAT TATGTACACA GAGACTTGGC GGCACGTAAT 
GTTCTTGTAA ATGCCCAATT AATATGCAAA ATCGCGGACT TCGGATTGTC ACGTGAAATT 
GAAAATGCAA GTGATGCATA CACAACTCGG GGCGGTAAAA TTCCTGTACG TTGGACTGCG 
CCGGAAGCCA TTGCTTTTAG AAAATTTACT TCAGCATCGG ATGTTTGGTC GTATGGAGTA 
GTTCTATGGG AGGTCATGTC CTATGGGGAA CGTCCATATT GGAACTGGTC AAATCAGGAC 
GTGATAAAGA GTATAGAAAA GGGCTACCGT TTACCAGCTC CGATGGATTG TCCAGAAGCC 
CTTTATCAGC TAATGTTGGA TTGCTGGCAA AAGCAACGTA CTCATCGCCC CACATTTGCA 
AGTATCGTTT CAACTCTTGA CAATTTGGCA CGACAACCAC AGTCATTATT GACAACACGA 
CCCTCGCCAG AATCAGACGG TAATCATATT TTGGATGGAC AGCGCGGTCA AAATATTTTC 
ATAAGCACTG ACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eph ( NM_166775.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726589.2 Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00276
Clone ID Related Accession (Same CDS sequence)	NM_166775.4 , NM_166776.4
Accession Version	NM_166775.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Eph receptor tyrosine kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166775.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTGACG ACGCCTTTAT TTGGAAATAG CAGAAGCTAC 
GTAGATCCGC ATACCTATGA AGATCCTAAT CAAGCTATAA GGGAATTCGC TCGAGAAATC 
GATGCAAACT ACATTACAAT AGAAGCTATT ATCGGAGGTG GAGAATTTGG CGATGTATGT 
CGTGGTCGAT TAAAAATACC CCCAAACTTT GTACAGGACA TTGATGTGGC AATAAAGACT 
TTAAAACCAG GGTCTTCGGA AAAAGCGCGC TGTGATTTCT TGACAGAGGC TTCTATTATG 
GGACAGTTTG ATCATCCAAA CGTTATTTAT CTACAGGGAG TAGTTACTCG GTCTAATCCA 
GTTATGATTA TTACTGAGTA CATGGAAAAT GGCAGCTTAG ACACATTTCT TCGGGTGAAT 
GATGGAAAGT TTCAAACATT ACAGCTTATA GTTATGCTTC GGGGTATAGC CAGCGGGATG 
TCATATTTAA GTGATATGAA TTATGTACAC AGAGACTTGG CGGCACGTAA TGTTCTTGTA 
AATGCCCAAT TAATATGCAA AATCGCGGAC TTCGGATTGT CACGTGAAAT TGAAAATGCA 
AGTGATGCAT ACACAACTCG GGGCGGTAAA ATTCCTGTAC GTTGGACTGC GCCGGAAGCC 
ATTGCTTTTA GAAAATTTAC TTCAGCATCG GATGTTTGGT CGTATGGAGT AGTTCTATGG 
GAGGTCATGT CCTATGGGGA ACGTCCATAT TGGAACTGGT CAAATCAGGA CGTGATAAAG 
AGTATAGAAA AGGGCTACCG TTTACCAGCT CCGATGGATT GTCCAGAAGC CCTTTATCAG 
CTAATGTTGG ATTGCTGGCA AAAGCAACGT ACTCATCGCC CCACATTTGC AAGTATCGTT 
TCAACTCTTG ACAATTTGGC ACGACAACCA CAGTCATTAT TGACAACACG ACCCTCGCCA 
GAATCAGACG GTAATCATAT TTTGGATGGA CAGCGCGGTC AAAATATTTT CATAAGCACT 
GACCTTTGGT TAGAGCACAT TAAAATGTCT AGATACTGTC ATCATTTTAA AGAGGCTAAT 
TTAATAAATG CTCAACAGAT ATCCAGGTTA ACAGCTCAAC AACTTTCGGA CATGGGAATT 
ACTTTAGTTG GACATCAAAA AAAGATCTTA CATCAAGCCC GGCAACTCGA CACTATAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726589.2
CDS	332..3574
Translation	MKMVGCHPNDKKVKYDGIKSKTSSVTILTSVLNDAISALKSAFYKNQAPISEQNRSQIVSRMSLLRTILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIAVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENASDAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKILHQARQLDTII

Target ORF information:

RefSeq Version	NM_166775.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166775.4

ORF Insert Sequence:

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTGACG ACGCCTTTAT TTGGAAATAG CAGAAGCTAC 
GTAGATCCGC ATACCTATGA AGATCCTAAT CAAGCTATAA GGGAATTCGC TCGAGAAATC 
GATGCAAACT ACATTACAAT AGAAGCTATT ATCGGAGGTG GAGAATTTGG CGATGTATGT 
CGTGGTCGAT TAAAAATACC CCCAAACTTT GTACAGGACA TTGATGTGGC AATAAAGACT 
TTAAAACCAG GGTCTTCGGA AAAAGCGCGC TGTGATTTCT TGACAGAGGC TTCTATTATG 
GGACAGTTTG ATCATCCAAA CGTTATTTAT CTACAGGGAG TAGTTACTCG GTCTAATCCA 
GTTATGATTA TTACTGAGTA CATGGAAAAT GGCAGCTTAG ACACATTTCT TCGGGTGAAT 
GATGGAAAGT TTCAAACATT ACAGCTTATA GTTATGCTTC GGGGTATAGC CAGCGGGATG 
TCATATTTAA GTGATATGAA TTATGTACAC AGAGACTTGG CGGCACGTAA TGTTCTTGTA 
AATGCCCAAT TAATATGCAA AATCGCGGAC TTCGGATTGT CACGTGAAAT TGAAAATGCA 
AGTGATGCAT ACACAACTCG GGGCGGTAAA ATTCCTGTAC GTTGGACTGC GCCGGAAGCC 
ATTGCTTTTA GAAAATTTAC TTCAGCATCG GATGTTTGGT CGTATGGAGT AGTTCTATGG 
GAGGTCATGT CCTATGGGGA ACGTCCATAT TGGAACTGGT CAAATCAGGA CGTGATAAAG 
AGTATAGAAA AGGGCTACCG TTTACCAGCT CCGATGGATT GTCCAGAAGC CCTTTATCAG 
CTAATGTTGG ATTGCTGGCA AAAGCAACGT ACTCATCGCC CCACATTTGC AAGTATCGTT 
TCAACTCTTG ACAATTTGGC ACGACAACCA CAGTCATTAT TGACAACACG ACCCTCGCCA 
GAATCAGACG GTAATCATAT TTTGGATGGA CAGCGCGGTC AAAATATTTT CATAAGCACT 
GACCTTTGGT TAGAGCACAT TAAAATGTCT AGATACTGTC ATCATTTTAA AGAGGCTAAT 
TTAATAAATG CTCAACAGAT ATCCAGGTTA ACAGCTCAAC AACTTTCGGA CATGGGAATT 
ACTTTAGTTG GACATCAAAA AAAGATCTTA CATCAAGCCC GGCAACTCGA CACTATAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eph ( NM_166776.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726590.2 Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa00276
Clone ID Related Accession (Same CDS sequence)	NM_166775.4 , NM_166776.4
Accession Version	NM_166776.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Eph receptor tyrosine kinase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166776.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTGACG ACGCCTTTAT TTGGAAATAG CAGAAGCTAC 
GTAGATCCGC ATACCTATGA AGATCCTAAT CAAGCTATAA GGGAATTCGC TCGAGAAATC 
GATGCAAACT ACATTACAAT AGAAGCTATT ATCGGAGGTG GAGAATTTGG CGATGTATGT 
CGTGGTCGAT TAAAAATACC CCCAAACTTT GTACAGGACA TTGATGTGGC AATAAAGACT 
TTAAAACCAG GGTCTTCGGA AAAAGCGCGC TGTGATTTCT TGACAGAGGC TTCTATTATG 
GGACAGTTTG ATCATCCAAA CGTTATTTAT CTACAGGGAG TAGTTACTCG GTCTAATCCA 
GTTATGATTA TTACTGAGTA CATGGAAAAT GGCAGCTTAG ACACATTTCT TCGGGTGAAT 
GATGGAAAGT TTCAAACATT ACAGCTTATA GTTATGCTTC GGGGTATAGC CAGCGGGATG 
TCATATTTAA GTGATATGAA TTATGTACAC AGAGACTTGG CGGCACGTAA TGTTCTTGTA 
AATGCCCAAT TAATATGCAA AATCGCGGAC TTCGGATTGT CACGTGAAAT TGAAAATGCA 
AGTGATGCAT ACACAACTCG GGGCGGTAAA ATTCCTGTAC GTTGGACTGC GCCGGAAGCC 
ATTGCTTTTA GAAAATTTAC TTCAGCATCG GATGTTTGGT CGTATGGAGT AGTTCTATGG 
GAGGTCATGT CCTATGGGGA ACGTCCATAT TGGAACTGGT CAAATCAGGA CGTGATAAAG 
AGTATAGAAA AGGGCTACCG TTTACCAGCT CCGATGGATT GTCCAGAAGC CCTTTATCAG 
CTAATGTTGG ATTGCTGGCA AAAGCAACGT ACTCATCGCC CCACATTTGC AAGTATCGTT 
TCAACTCTTG ACAATTTGGC ACGACAACCA CAGTCATTAT TGACAACACG ACCCTCGCCA 
GAATCAGACG GTAATCATAT TTTGGATGGA CAGCGCGGTC AAAATATTTT CATAAGCACT 
GACCTTTGGT TAGAGCACAT TAAAATGTCT AGATACTGTC ATCATTTTAA AGAGGCTAAT 
TTAATAAATG CTCAACAGAT ATCCAGGTTA ACAGCTCAAC AACTTTCGGA CATGGGAATT 
ACTTTAGTTG GACATCAAAA AAAGATCTTA CATCAAGCCC GGCAACTCGA CACTATAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726590.2
CDS	332..3574
Translation	MKMVGCHPNDKKVKYDGIKSKTSSVTILTSVLNDAISALKSAFYKNQAPISEQNRSQIVSRMSLLRTILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIAVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENASDAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKILHQARQLDTII

Target ORF information:

RefSeq Version	NM_166776.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166776.4

ORF Insert Sequence:

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTGACG ACGCCTTTAT TTGGAAATAG CAGAAGCTAC 
GTAGATCCGC ATACCTATGA AGATCCTAAT CAAGCTATAA GGGAATTCGC TCGAGAAATC 
GATGCAAACT ACATTACAAT AGAAGCTATT ATCGGAGGTG GAGAATTTGG CGATGTATGT 
CGTGGTCGAT TAAAAATACC CCCAAACTTT GTACAGGACA TTGATGTGGC AATAAAGACT 
TTAAAACCAG GGTCTTCGGA AAAAGCGCGC TGTGATTTCT TGACAGAGGC TTCTATTATG 
GGACAGTTTG ATCATCCAAA CGTTATTTAT CTACAGGGAG TAGTTACTCG GTCTAATCCA 
GTTATGATTA TTACTGAGTA CATGGAAAAT GGCAGCTTAG ACACATTTCT TCGGGTGAAT 
GATGGAAAGT TTCAAACATT ACAGCTTATA GTTATGCTTC GGGGTATAGC CAGCGGGATG 
TCATATTTAA GTGATATGAA TTATGTACAC AGAGACTTGG CGGCACGTAA TGTTCTTGTA 
AATGCCCAAT TAATATGCAA AATCGCGGAC TTCGGATTGT CACGTGAAAT TGAAAATGCA 
AGTGATGCAT ACACAACTCG GGGCGGTAAA ATTCCTGTAC GTTGGACTGC GCCGGAAGCC 
ATTGCTTTTA GAAAATTTAC TTCAGCATCG GATGTTTGGT CGTATGGAGT AGTTCTATGG 
GAGGTCATGT CCTATGGGGA ACGTCCATAT TGGAACTGGT CAAATCAGGA CGTGATAAAG 
AGTATAGAAA AGGGCTACCG TTTACCAGCT CCGATGGATT GTCCAGAAGC CCTTTATCAG 
CTAATGTTGG ATTGCTGGCA AAAGCAACGT ACTCATCGCC CCACATTTGC AAGTATCGTT 
TCAACTCTTG ACAATTTGGC ACGACAACCA CAGTCATTAT TGACAACACG ACCCTCGCCA 
GAATCAGACG GTAATCATAT TTTGGATGGA CAGCGCGGTC AAAATATTTT CATAAGCACT 
GACCTTTGGT TAGAGCACAT TAAAATGTCT AGATACTGTC ATCATTTTAA AGAGGCTAAT 
TTAATAAATG CTCAACAGAT ATCCAGGTTA ACAGCTCAAC AACTTTCGGA CATGGGAATT 
ACTTTAGTTG GACATCAAAA AAAGATCTTA CATCAAGCCC GGCAACTCGA CACTATAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eph ( NM_079886.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524625.3 Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00277
Clone ID Related Accession (Same CDS sequence)	NM_079886.5
Accession Version	NM_079886.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Eph receptor tyrosine kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_079886.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTTAAT TCTATGGATA CTACTCCAAT TGTTAAAAAA 
CTTCATTTAA ACGTGACGAC GCCTTTATTT GGAAATAGCA GAAGCTACGT AGATCCGCAT 
ACCTATGAAG ATCCTAATCA AGCTATAAGG GAATTCGCTC GAGAAATCGA TGCAAACTAC 
ATTACAATAG AAGCTATTAT CGGAGGTGGA GAATTTGGCG ATGTATGTCG TGGTCGATTA 
AAAATACCCC CAAACTTTGT ACAGGACATT GATGTGGCAA TAAAGACTTT AAAACCAGGG 
TCTTCGGAAA AAGCGCGCTG TGATTTCTTG ACAGAGGCTT CTATTATGGG ACAGTTTGAT 
CATCCAAACG TTATTTATCT ACAGGGAGTA GTTACTCGGT CTAATCCAGT TATGATTATT 
ACTGAGTACA TGGAAAATGG CAGCTTAGAC ACATTTCTTC GGGTGAATGA TGGAAAGTTT 
CAAACATTAC AGCTTATAGT TATGCTTCGG GGTATAGCCA GCGGGATGTC ATATTTAAGT 
GATATGAATT ATGTACACAG AGACTTGGCG GCACGTAATG TTCTTGTAAA TGCCCAATTA 
ATATGCAAAA TCGCGGACTT CGGATTGTCA CGTGAAATTG AAAATGCAAG TGATGCATAC 
ACAACTCGGG GCGGTAAAAT TCCTGTACGT TGGACTGCGC CGGAAGCCAT TGCTTTTAGA 
AAATTTACTT CAGCATCGGA TGTTTGGTCG TATGGAGTAG TTCTATGGGA GGTCATGTCC 
TATGGGGAAC GTCCATATTG GAACTGGTCA AATCAGGACG TGATAAAGAG TATAGAAAAG 
GGCTACCGTT TACCAGCTCC GATGGATTGT CCAGAAGCCC TTTATCAGCT AATGTTGGAT 
TGCTGGCAAA AGCAACGTAC TCATCGCCCC ACATTTGCAA GTATCGTTTC AACTCTTGAC 
AATTTGGCAC GACAACCACA GTCATTATTG ACAACACGAC CCTCGCCAGA ATCAGACGGT 
AATCATATTT TGGATGGACA GCGCGGTCAA AATATTTTCA TAAGCACTGA CCTTTGGTTA 
GAGCACATTA AAATGTCTAG ATACTGTCAT CATTTTAAAG AGGCTAATTT AATAAATGCT 
CAACAGATAT CCAGGTTAAC AGCTCAACAA CTTTCGGACA TGGGAATTAC TTTAGTTGGA 
CATCAAAAAA AGATCTTACA TCAAGCCCGG CAACTCGACA CTATAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524625.3
CDS	332..3622
Translation	MKMVGCHPNDKKVKYDGIKSKTSSVTILTSVLNDAISALKSAFYKNQAPISEQNRSQIVSRMSLLRTILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIAVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENASDAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKILHQARQLDTII

Target ORF information:

RefSeq Version	NM_079886.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079886.5

ORF Insert Sequence:

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTTAAT TCTATGGATA CTACTCCAAT TGTTAAAAAA 
CTTCATTTAA ACGTGACGAC GCCTTTATTT GGAAATAGCA GAAGCTACGT AGATCCGCAT 
ACCTATGAAG ATCCTAATCA AGCTATAAGG GAATTCGCTC GAGAAATCGA TGCAAACTAC 
ATTACAATAG AAGCTATTAT CGGAGGTGGA GAATTTGGCG ATGTATGTCG TGGTCGATTA 
AAAATACCCC CAAACTTTGT ACAGGACATT GATGTGGCAA TAAAGACTTT AAAACCAGGG 
TCTTCGGAAA AAGCGCGCTG TGATTTCTTG ACAGAGGCTT CTATTATGGG ACAGTTTGAT 
CATCCAAACG TTATTTATCT ACAGGGAGTA GTTACTCGGT CTAATCCAGT TATGATTATT 
ACTGAGTACA TGGAAAATGG CAGCTTAGAC ACATTTCTTC GGGTGAATGA TGGAAAGTTT 
CAAACATTAC AGCTTATAGT TATGCTTCGG GGTATAGCCA GCGGGATGTC ATATTTAAGT 
GATATGAATT ATGTACACAG AGACTTGGCG GCACGTAATG TTCTTGTAAA TGCCCAATTA 
ATATGCAAAA TCGCGGACTT CGGATTGTCA CGTGAAATTG AAAATGCAAG TGATGCATAC 
ACAACTCGGG GCGGTAAAAT TCCTGTACGT TGGACTGCGC CGGAAGCCAT TGCTTTTAGA 
AAATTTACTT CAGCATCGGA TGTTTGGTCG TATGGAGTAG TTCTATGGGA GGTCATGTCC 
TATGGGGAAC GTCCATATTG GAACTGGTCA AATCAGGACG TGATAAAGAG TATAGAAAAG 
GGCTACCGTT TACCAGCTCC GATGGATTGT CCAGAAGCCC TTTATCAGCT AATGTTGGAT 
TGCTGGCAAA AGCAACGTAC TCATCGCCCC ACATTTGCAA GTATCGTTTC AACTCTTGAC 
AATTTGGCAC GACAACCACA GTCATTATTG ACAACACGAC CCTCGCCAGA ATCAGACGGT 
AATCATATTT TGGATGGACA GCGCGGTCAA AATATTTTCA TAAGCACTGA CCTTTGGTTA 
GAGCACATTA AAATGTCTAG ATACTGTCAT CATTTTAAAG AGGCTAATTT AATAAATGCT 
CAACAGATAT CCAGGTTAAC AGCTCAACAA CTTTCGGACA TGGGAATTAC TTTAGTTGGA 
CATCAAAAAA AGATCTTACA TCAAGCCCGG CAACTCGACA CTATAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eph ( NM_166777.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726591.2 Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant B, mRNA.

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CloneID	OFa00278
Clone ID Related Accession (Same CDS sequence)	NM_166777.4
Accession Version	NM_166777.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Eph receptor tyrosine kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166777.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAAAA GCGCCCTCAA GTCTGCGTTT TACAAAAATC AAGCTCCAAT TAGCGAGCAA 
AACAGATCTC AAATTGTTTC GAGAATGTCA TTATTAAGGA CAATACTGCT TATTATAATT 
TCAATTCACT TTAAATTGGC ACATGCAGAT CAGGTTGTTC TATTGGATAC AACTCGAGAA 
GCTACATTAG AGTGGACCAG GTATCCATAT GGACCTCAAG CCCAAACACC AGGGTGGGTG 
GAGGAGTCAT TTACCGACTT CGTTAAGGGC ATTAACTGGA GGAGCTATGT GGTTTGCGAT 
GTGGCATATC ACAACGTCAA TAACTGGTTG TGGTCGCCGT TTATCGATCG TGGATCAGCA 
AACCGACTTT ACATTGAAAT TCAGTTCACA ATACGCGATT GTTCTTTATT CCCAGGAAAC 
GCCTTGTCAT GCAAGGAAAC ATTTAGCTTA CTTTTTTACG AGTTTGATGC GGCTACTAGA 
GAACCTCCAC CATGGCAAAC AGACAGCTAC AGGCTGATTG CTCGCATCGC GGCTGGCGAG 
GGGCGCTTCA ATCAGAACTC CGATGTAGAT ATTAACACAG AGGTGAAGAG TATAGCCGTC 
AACAAAAAAG GCGTATATTT TGCTTTCAGA GATCAGGGCG CTTGTATTAG CGTTCTGGCA 
GTAAAAGTAT ATTATATTAC GTGTCCTGCT GTTACCGAAA ACTTTGCACA CTTTAACGAA 
ACACCTACGG GAAGAGAAAT TACTATAATT GAGAAACAAA ATGGAACATG TGTCGATAAC 
GCAGAACCGT ATGAGACGCC AACTTACTTA TGTAAAGGTG ATGGAAAATG GACAATTCTT 
ACTGGTGGGT GTCGTTGCAA GGCTGGCTAT GAGCCGAATT ACACAAATAA GACTTGTACA 
GAATGTCCAC TTGGGACATT TAAATCCCCA GAGGTTACAA AATGTACTCC GTGTCCTCCC 
AATTCAAATG CTTCAAAAAC TGGTTCACCT TTCTGTAAGT GTGCGTCTGG ATTCTATAGA 
CATCCAAATG ATGGTCGACA TATGCCGTGT TATAGTCCAC CAGCTGCGCC TACTAATCTC 
ACGTTGTTGT TTGTTGATCA AACAAGTGCA ATAATATCTT GGAGCGCACC GGCCAAAAAT 
GAATCGTTTT CATCTGAAAC TAATTCTAAA ATATATCACA GTGATATTGT GTACAAGATC 
AAGTGTAATA TATGCAGCCC AAATGTGGTT TACAATCCCT CAACTGACAC ATTTAACGAA 
ACAAAAATAA CACTTACAAA TCTGGAACCG GTAACGACAT ACACTGTGCA GATACACGCA 
ATAAATAGCG TGTCTCATAT TAACGAATTT AAAAGGCACT CCAACGAGTC TTCCCTGGTG 
GCAGTCAGTG ACATAGTATT TTCAAATACG TCTTTATTAA ATATACCTTT GGATTTAAAC 
GAAGTAAAGA CTGGTCAAGC TGAAATCGTT TTTACAACTG AGTCAGTTTT GCTCTCGACA 
GTTTTTAATT TGCGTATATT GGCTATAACT AATAAAGATG CCGATCTAGA ATGGGATAAA 
CCTGTTCAAA GTGATTTTCC ACTTGAATTT TATGAAGTGC GCTGGTTTCC AAAGGTCGAA 
TTAGATGCTA TAAATAAATC AGCTTTGAAC ACAAAAGAAA CGAAGGCACA TATAGTAGGA 
CTTCTAGAAA ATACGGAGTA TGGATTTCAA GTTCGTTGTA AAACGAATAA TGGATTTGGC 
TCTTATAGTA ACATGATTTA CGCCCAAACT CTTCAATCCG TTGGTTCAGT ATATGACGAT 
TCTGTGCAAA TACGATTTAT TGCTGGAGCC ATCGTGACGG GAGTATTATT TCTAGTCATT 
TTTATAATAG CTACAGTATA TTTCATGAGA TCGAAGCATC AGGATGATCT TGATAAAAAA 
TCGACAAATC ATTTGCCATT ACCACTTGAC TACGCCAGCA ACGAAGTGAC GACGCCTTTA 
TTTGGAAATA GCAGAAGCTA CGTAGATCCG CATACCTATG AAGATCCTAA TCAAGCTATA 
AGGGAATTCG CTCGAGAAAT CGATGCAAAC TACATTACAA TAGAAGCTAT TATCGGAGGT 
GGAGAATTTG GCGATGTATG TCGTGGTCGA TTAAAAATAC CCCCAAACTT TGTACAGGAC 
ATTGATGTGG CAATAAAGAC TTTAAAACCA GGGTCTTCGG AAAAAGCGCG CTGTGATTTC 
TTGACAGAGG CTTCTATTAT GGGACAGTTT GATCATCCAA ACGTTATTTA TCTACAGGGA 
GTAGTTACTC GGTCTAATCC AGTTATGATT ATTACTGAGT ACATGGAAAA TGGCAGCTTA 
GACACATTTC TTCGGGTGAA TGATGGAAAG TTTCAAACAT TACAGCTTAT AGTTATGCTT 
CGGGGTATAG CCAGCGGGAT GTCATATTTA AGTGATATGA ATTATGTACA CAGAGACTTG 
GCGGCACGTA ATGTTCTTGT AAATGCCCAA TTAATATGCA AAATCGCGGA CTTCGGATTG 
TCACGTGAAA TTGAAAATGC AAGTGATGCA TACACAACTC GGGGCGGTAA AATTCCTGTA 
CGTTGGACTG CGCCGGAAGC CATTGCTTTT AGAAAATTTA CTTCAGCATC GGATGTTTGG 
TCGTATGGAG TAGTTCTATG GGAGGTCATG TCCTATGGGG AACGTCCATA TTGGAACTGG 
TCAAATCAGG ACGTGATAAA GAGTATAGAA AAGGGCTACC GTTTACCAGC TCCGATGGAT 
TGTCCAGAAG CCCTTTATCA GCTAATGTTG GATTGCTGGC AAAAGCAACG TACTCATCGC 
CCCACATTTG CAAGTATCGT TTCAACTCTT GACAATTTGG CACGACAACC ACAGTCATTA 
TTGACAACAC GACCCTCGCC AGAATCAGAC GGTAATCATA TTTTGGATGG ACAGCGCGGT 
CAAAATATTT TCATAAGCAC TGACCTTTGG TTAGAGCACA TTAAAATGTC TAGATACTGT 
CATCATTTTA AAGAGGCTAA TTTAATAAAT GCTCAACAGA TATCCAGGTT AACAGCTCAA 
CAACTTTCGG ACATGGGAAT TACTTTAGTT GGACATCAAA AAAAGATCTT ACATCAAGCC 
CGGCAACTCG ACACTATAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726591.2
CDS	357..3500
Translation	MTKSALKSAFYKNQAPISEQNRSQIVSRMSLLRTILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIAVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENASDAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKILHQARQLDTII

Target ORF information:

RefSeq Version	NM_166777.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166777.4

ORF Insert Sequence:

ATGACAAAAA GCGCCCTCAA GTCTGCGTTT TACAAAAATC AAGCTCCAAT TAGCGAGCAA 
AACAGATCTC AAATTGTTTC GAGAATGTCA TTATTAAGGA CAATACTGCT TATTATAATT 
TCAATTCACT TTAAATTGGC ACATGCAGAT CAGGTTGTTC TATTGGATAC AACTCGAGAA 
GCTACATTAG AGTGGACCAG GTATCCATAT GGACCTCAAG CCCAAACACC AGGGTGGGTG 
GAGGAGTCAT TTACCGACTT CGTTAAGGGC ATTAACTGGA GGAGCTATGT GGTTTGCGAT 
GTGGCATATC ACAACGTCAA TAACTGGTTG TGGTCGCCGT TTATCGATCG TGGATCAGCA 
AACCGACTTT ACATTGAAAT TCAGTTCACA ATACGCGATT GTTCTTTATT CCCAGGAAAC 
GCCTTGTCAT GCAAGGAAAC ATTTAGCTTA CTTTTTTACG AGTTTGATGC GGCTACTAGA 
GAACCTCCAC CATGGCAAAC AGACAGCTAC AGGCTGATTG CTCGCATCGC GGCTGGCGAG 
GGGCGCTTCA ATCAGAACTC CGATGTAGAT ATTAACACAG AGGTGAAGAG TATAGCCGTC 
AACAAAAAAG GCGTATATTT TGCTTTCAGA GATCAGGGCG CTTGTATTAG CGTTCTGGCA 
GTAAAAGTAT ATTATATTAC GTGTCCTGCT GTTACCGAAA ACTTTGCACA CTTTAACGAA 
ACACCTACGG GAAGAGAAAT TACTATAATT GAGAAACAAA ATGGAACATG TGTCGATAAC 
GCAGAACCGT ATGAGACGCC AACTTACTTA TGTAAAGGTG ATGGAAAATG GACAATTCTT 
ACTGGTGGGT GTCGTTGCAA GGCTGGCTAT GAGCCGAATT ACACAAATAA GACTTGTACA 
GAATGTCCAC TTGGGACATT TAAATCCCCA GAGGTTACAA AATGTACTCC GTGTCCTCCC 
AATTCAAATG CTTCAAAAAC TGGTTCACCT TTCTGTAAGT GTGCGTCTGG ATTCTATAGA 
CATCCAAATG ATGGTCGACA TATGCCGTGT TATAGTCCAC CAGCTGCGCC TACTAATCTC 
ACGTTGTTGT TTGTTGATCA AACAAGTGCA ATAATATCTT GGAGCGCACC GGCCAAAAAT 
GAATCGTTTT CATCTGAAAC TAATTCTAAA ATATATCACA GTGATATTGT GTACAAGATC 
AAGTGTAATA TATGCAGCCC AAATGTGGTT TACAATCCCT CAACTGACAC ATTTAACGAA 
ACAAAAATAA CACTTACAAA TCTGGAACCG GTAACGACAT ACACTGTGCA GATACACGCA 
ATAAATAGCG TGTCTCATAT TAACGAATTT AAAAGGCACT CCAACGAGTC TTCCCTGGTG 
GCAGTCAGTG ACATAGTATT TTCAAATACG TCTTTATTAA ATATACCTTT GGATTTAAAC 
GAAGTAAAGA CTGGTCAAGC TGAAATCGTT TTTACAACTG AGTCAGTTTT GCTCTCGACA 
GTTTTTAATT TGCGTATATT GGCTATAACT AATAAAGATG CCGATCTAGA ATGGGATAAA 
CCTGTTCAAA GTGATTTTCC ACTTGAATTT TATGAAGTGC GCTGGTTTCC AAAGGTCGAA 
TTAGATGCTA TAAATAAATC AGCTTTGAAC ACAAAAGAAA CGAAGGCACA TATAGTAGGA 
CTTCTAGAAA ATACGGAGTA TGGATTTCAA GTTCGTTGTA AAACGAATAA TGGATTTGGC 
TCTTATAGTA ACATGATTTA CGCCCAAACT CTTCAATCCG TTGGTTCAGT ATATGACGAT 
TCTGTGCAAA TACGATTTAT TGCTGGAGCC ATCGTGACGG GAGTATTATT TCTAGTCATT 
TTTATAATAG CTACAGTATA TTTCATGAGA TCGAAGCATC AGGATGATCT TGATAAAAAA 
TCGACAAATC ATTTGCCATT ACCACTTGAC TACGCCAGCA ACGAAGTGAC GACGCCTTTA 
TTTGGAAATA GCAGAAGCTA CGTAGATCCG CATACCTATG AAGATCCTAA TCAAGCTATA 
AGGGAATTCG CTCGAGAAAT CGATGCAAAC TACATTACAA TAGAAGCTAT TATCGGAGGT 
GGAGAATTTG GCGATGTATG TCGTGGTCGA TTAAAAATAC CCCCAAACTT TGTACAGGAC 
ATTGATGTGG CAATAAAGAC TTTAAAACCA GGGTCTTCGG AAAAAGCGCG CTGTGATTTC 
TTGACAGAGG CTTCTATTAT GGGACAGTTT GATCATCCAA ACGTTATTTA TCTACAGGGA 
GTAGTTACTC GGTCTAATCC AGTTATGATT ATTACTGAGT ACATGGAAAA TGGCAGCTTA 
GACACATTTC TTCGGGTGAA TGATGGAAAG TTTCAAACAT TACAGCTTAT AGTTATGCTT 
CGGGGTATAG CCAGCGGGAT GTCATATTTA AGTGATATGA ATTATGTACA CAGAGACTTG 
GCGGCACGTA ATGTTCTTGT AAATGCCCAA TTAATATGCA AAATCGCGGA CTTCGGATTG 
TCACGTGAAA TTGAAAATGC AAGTGATGCA TACACAACTC GGGGCGGTAA AATTCCTGTA 
CGTTGGACTG CGCCGGAAGC CATTGCTTTT AGAAAATTTA CTTCAGCATC GGATGTTTGG 
TCGTATGGAG TAGTTCTATG GGAGGTCATG TCCTATGGGG AACGTCCATA TTGGAACTGG 
TCAAATCAGG ACGTGATAAA GAGTATAGAA AAGGGCTACC GTTTACCAGC TCCGATGGAT 
TGTCCAGAAG CCCTTTATCA GCTAATGTTG GATTGCTGGC AAAAGCAACG TACTCATCGC 
CCCACATTTG CAAGTATCGT TTCAACTCTT GACAATTTGG CACGACAACC ACAGTCATTA 
TTGACAACAC GACCCTCGCC AGAATCAGAC GGTAATCATA TTTTGGATGG ACAGCGCGGT 
CAAAATATTT TCATAAGCAC TGACCTTTGG TTAGAGCACA TTAAAATGTC TAGATACTGT 
CATCATTTTA AAGAGGCTAA TTTAATAAAT GCTCAACAGA TATCCAGGTT AACAGCTCAA 
CAACTTTCGG ACATGGGAAT TACTTTAGTT GGACATCAAA AAAAGATCTT ACATCAAGCC 
CGGCAACTCG ACACTATAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eph ( NM_166774.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726588.2 Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant D, mRNA.

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CloneID	OFa00279
Clone ID Related Accession (Same CDS sequence)	NM_166774.3
Accession Version	NM_166774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Eph receptor tyrosine kinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166774.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTTAAT TCTATGGATA CTACTCCAAT TGTTAAAAAA 
CTTCATTTAA ACGTGACGAC GCCTTTATTT GGAAATAGCA GAAGCTACGT AGATCCGCAT 
ACCTATGAAG ATCCTAATCA AGCTATAAGG GAATTCGCTC GAGAAATCGA TGCAAACTAC 
ATTACAATAG AAGCTATTAT CGGAGGTGGA GAATTTGGCG ATGTATGTCG TGGTCGATTA 
AAAATACCCC CAAACTTTGT ACAGGACATT GATGTGGCAA TAAAGACTTT AAAACCAGGG 
TCTTCGGAAA AAGCGCGCTG TGATTTCTTG ACAGAGGCTT CTATTATGGG ACAGTTTGAT 
CATCCAAACG TTATTTATCT ACAGGGAGTA GTTACTCGGT CTAATCCAGT TATGATTATT 
ACTGAGTACA TGGAAAATGG CAGCTTAGAC ACATTTCTTC GGGTGAATGA TGGAAAGTTT 
CAAACATTAC AGCTTATAGT TATGCTTCGG GGTATAGCCA GCGGGATGTC ATATTTAAGT 
GATATGAATT ATGTACACAG AGACTTGGCG GCACGTAATG TTCTTGTAAA TGCCCAATTA 
ATATGCAAAA TCGCGGACTT CGGATTGTCA CGTGAAATTG AAAATGCAAG TGATGCATAC 
ACAACTCGGG GCGGTAAAAT TCCTGTACGT TGGACTGCGC CGGAAGCCAT TGCTTTTAGA 
AAATTTACTT CAGCATCGGA TGTTTGGTCG TATGGAGTAG TTCTATGGGA GGTCATGTCC 
TATGGGGAAC GTCCATATTG GAACTGGTCA AATCAGGACG TGATAAAGAG TATAGAAAAG 
GGCTACCGTT TACCAGCTCC GATGGATTGT CCAGAAGCCC TTTATCAGCT AATGTTGGAT 
TGCTGGCAAA AGCAACGTAC TCATCGCCCC ACATTTGCAA GTATCGTTTC AACTCTTGAC 
AATTTGGCAC GACAACCACA GTCATTATTG ACAACACGAC CCTCGCCAGA ATCAGACGGT 
AATCATATTT TGGATGGACA GCGCGGTCAA AATATTTTCA TAAGCACTGA CCTTTGGTTA 
GAGCACATTA AAATGTCTAG ATACTGTCAT CATTTTAAAG AGGCTAATTT AATAAATGCT 
CAACAGGTAA ACTCTTCTAA TTATCATTTT TGTATTAATC AATTTTTTAT TGTCAAATAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726588.2
CDS	332..3574
Translation	MKMVGCHPNDKKVKYDGIKSKTSSVTILTSVLNDAISALKSAFYKNQAPISEQNRSQIVSRMSLLRTILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIAVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENASDAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQVNSSNYHFCINQFFIVKY

Target ORF information:

RefSeq Version	NM_166774.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Eph receptor tyrosine kinase (Eph), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166774.3

ORF Insert Sequence:

ATGAAAATGG TTGGGTGCCA CCCAAATGAC AAAAAGGTGA AATATGATGG TATTAAAAGT 
AAAACTTCTT CCGTAACCAT TCTAACTTCC GTACTAAATG ATGCTATAAG CGCCCTCAAG 
TCTGCGTTTT ACAAAAATCA AGCTCCAATT AGCGAGCAAA ACAGATCTCA AATTGTTTCG 
AGAATGTCAT TATTAAGGAC AATACTGCTT ATTATAATTT CAATTCACTT TAAATTGGCA 
CATGCAGATC AGGTTGTTCT ATTGGATACA ACTCGAGAAG CTACATTAGA GTGGACCAGG 
TATCCATATG GACCTCAAGC CCAAACACCA GGGTGGGTGG AGGAGTCATT TACCGACTTC 
GTTAAGGGCA TTAACTGGAG GAGCTATGTG GTTTGCGATG TGGCATATCA CAACGTCAAT 
AACTGGTTGT GGTCGCCGTT TATCGATCGT GGATCAGCAA ACCGACTTTA CATTGAAATT 
CAGTTCACAA TACGCGATTG TTCTTTATTC CCAGGAAACG CCTTGTCATG CAAGGAAACA 
TTTAGCTTAC TTTTTTACGA GTTTGATGCG GCTACTAGAG AACCTCCACC ATGGCAAACA 
GACAGCTACA GGCTGATTGC TCGCATCGCG GCTGGCGAGG GGCGCTTCAA TCAGAACTCC 
GATGTAGATA TTAACACAGA GGTGAAGAGT ATAGCCGTCA ACAAAAAAGG CGTATATTTT 
GCTTTCAGAG ATCAGGGCGC TTGTATTAGC GTTCTGGCAG TAAAAGTATA TTATATTACG 
TGTCCTGCTG TTACCGAAAA CTTTGCACAC TTTAACGAAA CACCTACGGG AAGAGAAATT 
ACTATAATTG AGAAACAAAA TGGAACATGT GTCGATAACG CAGAACCGTA TGAGACGCCA 
ACTTACTTAT GTAAAGGTGA TGGAAAATGG ACAATTCTTA CTGGTGGGTG TCGTTGCAAG 
GCTGGCTATG AGCCGAATTA CACAAATAAG ACTTGTACAG AATGTCCACT TGGGACATTT 
AAATCCCCAG AGGTTACAAA ATGTACTCCG TGTCCTCCCA ATTCAAATGC TTCAAAAACT 
GGTTCACCTT TCTGTAAGTG TGCGTCTGGA TTCTATAGAC ATCCAAATGA TGGTCGACAT 
ATGCCGTGTT ATAGTCCACC AGCTGCGCCT ACTAATCTCA CGTTGTTGTT TGTTGATCAA 
ACAAGTGCAA TAATATCTTG GAGCGCACCG GCCAAAAATG AATCGTTTTC ATCTGAAACT 
AATTCTAAAA TATATCACAG TGATATTGTG TACAAGATCA AGTGTAATAT ATGCAGCCCA 
AATGTGGTTT ACAATCCCTC AACTGACACA TTTAACGAAA CAAAAATAAC ACTTACAAAT 
CTGGAACCGG TAACGACATA CACTGTGCAG ATACACGCAA TAAATAGCGT GTCTCATATT 
AACGAATTTA AAAGGCACTC CAACGAGTCT TCCCTGGTGG CAGTCAGTGA CATAGTATTT 
TCAAATACGT CTTTATTAAA TATACCTTTG GATTTAAACG AAGTAAAGAC TGGTCAAGCT 
GAAATCGTTT TTACAACTGA GTCAGTTTTG CTCTCGACAG TTTTTAATTT GCGTATATTG 
GCTATAACTA ATAAAGATGC CGATCTAGAA TGGGATAAAC CTGTTCAAAG TGATTTTCCA 
CTTGAATTTT ATGAAGTGCG CTGGTTTCCA AAGGTCGAAT TAGATGCTAT AAATAAATCA 
GCTTTGAACA CAAAAGAAAC GAAGGCACAT ATAGTAGGAC TTCTAGAAAA TACGGAGTAT 
GGATTTCAAG TTCGTTGTAA AACGAATAAT GGATTTGGCT CTTATAGTAA CATGATTTAC 
GCCCAAACTC TTCAATCCGT TGGTTCAGTA TATGACGATT CTGTGCAAAT ACGATTTATT 
GCTGGAGCCA TCGTGACGGG AGTATTATTT CTAGTCATTT TTATAATAGC TACAGTATAT 
TTCATGAGAT CGAAGCATCA GGATGATCTT GATAAAAAAT CGACAAATCA TTTGCCATTA 
CCACTTGACT ACGCCAGCAA CGAAGTTAAT TCTATGGATA CTACTCCAAT TGTTAAAAAA 
CTTCATTTAA ACGTGACGAC GCCTTTATTT GGAAATAGCA GAAGCTACGT AGATCCGCAT 
ACCTATGAAG ATCCTAATCA AGCTATAAGG GAATTCGCTC GAGAAATCGA TGCAAACTAC 
ATTACAATAG AAGCTATTAT CGGAGGTGGA GAATTTGGCG ATGTATGTCG TGGTCGATTA 
AAAATACCCC CAAACTTTGT ACAGGACATT GATGTGGCAA TAAAGACTTT AAAACCAGGG 
TCTTCGGAAA AAGCGCGCTG TGATTTCTTG ACAGAGGCTT CTATTATGGG ACAGTTTGAT 
CATCCAAACG TTATTTATCT ACAGGGAGTA GTTACTCGGT CTAATCCAGT TATGATTATT 
ACTGAGTACA TGGAAAATGG CAGCTTAGAC ACATTTCTTC GGGTGAATGA TGGAAAGTTT 
CAAACATTAC AGCTTATAGT TATGCTTCGG GGTATAGCCA GCGGGATGTC ATATTTAAGT 
GATATGAATT ATGTACACAG AGACTTGGCG GCACGTAATG TTCTTGTAAA TGCCCAATTA 
ATATGCAAAA TCGCGGACTT CGGATTGTCA CGTGAAATTG AAAATGCAAG TGATGCATAC 
ACAACTCGGG GCGGTAAAAT TCCTGTACGT TGGACTGCGC CGGAAGCCAT TGCTTTTAGA 
AAATTTACTT CAGCATCGGA TGTTTGGTCG TATGGAGTAG TTCTATGGGA GGTCATGTCC 
TATGGGGAAC GTCCATATTG GAACTGGTCA AATCAGGACG TGATAAAGAG TATAGAAAAG 
GGCTACCGTT TACCAGCTCC GATGGATTGT CCAGAAGCCC TTTATCAGCT AATGTTGGAT 
TGCTGGCAAA AGCAACGTAC TCATCGCCCC ACATTTGCAA GTATCGTTTC AACTCTTGAC 
AATTTGGCAC GACAACCACA GTCATTATTG ACAACACGAC CCTCGCCAGA ATCAGACGGT 
AATCATATTT TGGATGGACA GCGCGGTCAA AATATTTTCA TAAGCACTGA CCTTTGGTTA 
GAGCACATTA AAATGTCTAG ATACTGTCAT CATTTTAAAG AGGCTAATTT AATAAATGCT 
CAACAGGTAA ACTCTTCTAA TTATCATTTT TGTATTAATC AATTTTTTAT TGTCAAATAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eph cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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