Vtcn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vtcn1
Entrez Gene ID	242122
Full Name	V-set domain containing T cell activation inhibitor 1
Synonyms	B7h4,B7s1,B7x,BC032925
General protein information	Preferred Names V-set domain containing T cell activation inhibitor 1 Names V-set domain containing T-cell activation inhibitor 1 B7 homolog 4 immune costimulatory protein B7-H4 t cell costimulatory molecule B7x vtcn1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_178594.3	NP_848709.2	V-set domain containing T-cell activation inhibitor 1 precursor
XM_030252620.1	XP_030108480.1	V-set domain containing T-cell activation inhibitor 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vtcn1	NP_001019415.1
Bos taurus (cattle)	VTCN1	XP_002686167.2
Homo sapiens (human)	VTCN1	NP_078902.2
Canis lupus familiaris (dog)	VTCN1	XP_005630720.1
Gallus gallus (chicken)	VTCN1	XP_416546.4
	LOC714123	XP_001103715.1
Mus musculus (house mouse)	Vtcn1	NP_848709.2
Xenopus tropicalis (tropical clawed frog)	vtcn1	NP_001008189.1
Pan troglodytes (chimpanzee)	VTCN1	XP_003308340.1
Danio rerio (zebrafish)	si:dkey-174k18.6	XP_003199259.1

Gene Ontology
Bibliography

Related articles in PubMed

B7-H4 enhances the differentiation of murine leukemia-initiating cells via the PTEN/AKT/RCOR2/RUNX1 pathways.
Xia F, Zhang Y, Xie L, Jiang H, Zeng H, Chen C, Liu L, He X, Hao X, Fang X, Liu X, Zhang F, Gu H, Wan J, Cheng Y, Zhang CC, Chen GQ, Lu Y, Yu Z, Zheng J
Leukemia31(10)2260-2264(2017 Oct)

Synergistic effects of host B7-H4 deficiency and gemcitabine treatment on tumor regression and anti-tumor T cell immunity in a mouse model.
Leung J, St-Onge P, Stagg J, Suh WK
Cancer immunology, immunotherapy : CII66(4)491-502(2017 Apr)

Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.
Radichev IA, Maneva-Radicheva LV, Amatya C, Salehi M, Parker C, Ellefson J, Burn P, Savinov AY
Journal of immunology (Baltimore, Md. : 1950)196(4)1495-506(2016 Feb)

Ablation of B7-H3 but Not B7-H4 Results in Highly Increased Tumor Burden in a Murine Model of Spontaneous Prostate Cancer.
Kreymborg K, Haak S, Murali R, Wei J, Waitz R, Gasteiger G, Savage PA, van den Brink MR, Allison JP
Cancer immunology research3(8)849-54(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that B7-H4 knockout hosts mount greater tumor-associated anti-tumor T cell responses and display reduced tumors
Title: Synergistic effects of host B7-H4 deficiency and gemcitabine treatment on tumor regression and anti-tumor T cell immunity in a mouse model.

Results highlight an importance of VTCN1 stabilization on cell surfaces for the restoration of altered balance of immune control during T1D.
Title: Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.

Ablation of B7-H3 but Not B7-H4 Results in Highly Increased Tumor Burden in a Murine Model of Spontaneous Prostate Cancer
Title: Ablation of B7-H3 but Not B7-H4 Results in Highly Increased Tumor Burden in a Murine Model of Spontaneous Prostate Cancer.

B7-H4 expression by nonhematopoietic cells in the tumor microenvironment promotes antitumor immunity.
Title: B7-H4 expression by nonhematopoietic cells in the tumor microenvironment promotes antitumor immunity.

that B7x can modulate kidney damage in autoimmune diseases including lupus nephritis and anti-glomerular basement membrane disease
Title: B7x/B7-H4 modulates the adaptive immune response and ameliorates renal injury in antibody-mediated nephritis.

The following Vtcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vtcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18103	NM_178594.3 Latest version!	Mus musculus V-set domain containing T cell activation inhibitor 1 (Vtcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu88226	XM_030252620.1 Latest version!	Mus musculus V-set domain containing T cell activation inhibitor 1 (Vtcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vtcn1 ( NM_178594.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848709.2 Mus musculus V-set domain containing T cell activation inhibitor 1 (Vtcn1), mRNA.

Price & Availability↑

CloneID	OMu18103
Clone ID Related Accession (Same CDS sequence)	NM_178594.3
Accession Version	NM_178594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	V-set domain containing T-cell activation inhibitor 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK145664.1. On Oct 1, 2009 this sequence version replaced NM_178594.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145664.1, AK143276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCCT TGGGGCAGAT CATCTTTTGG AGTATTATTA ACATCATCAT CATCCTGGCT 
GGGGCCATCG CACTCATCAT TGGCTTTGGC ATTTCAGGCA AGCACTTCAT CACGGTCACG 
ACCTTCACCT CAGCTGGAAA CATTGGAGAG GACGGGACCC TGAGCTGCAC TTTTGAACCT 
GACATCAAAC TCAACGGCAT CGTCATCCAG TGGCTGAAAG AAGGCATCAA AGGTTTGGTC 
CACGAGTTCA AAGAAGGCAA AGACGACCTC TCACAGCAGC ATGAGATGTT CAGAGGCCGC 
ACAGCAGTGT TTGCTGATCA GGTGGTAGTT GGCAATGCTT CCCTGAGACT GAAAAACGTG 
CAGCTCACGG ATGCTGGCAC CTACACATGT TACATCCGCA CCTCAAAAGG CAAAGGGAAT 
GCAAACCTTG AGTATAAGAC CGGAGCCTTC AGTATGCCAG AGATAAATGT GGACTATAAT 
GCCAGTTCAG AGAGTTTACG CTGCGAGGCT CCTCGGTGGT TCCCCCAGCC CACAGTGGCC 
TGGGCATCTC AAGTCGACCA AGGAGCCAAT TTCTCAGAAG TCTCCAACAC CAGCTTTGAG 
TTGAACTCTG AGAATGTGAC CATGAAGGTC GTATCTGTGC TCTACAATGT CACAATCAAC 
AACACATACT CCTGTATGAT TGAAAACGAC ATTGCCAAAG CCACCGGGGA CATCAAAGTG 
ACAGATTCAG AGGTCAAAAG GCGAAGTCAG CTGCAGTTGC TGAACTCTGG GCCTTCCCCG 
TGTGTTTTTT CTTCTGCCTT TGTGGCTGGC TGGGCACTCC TATCTCTCTC CTGTTGCCTG 
ATGCTAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848709.2
CDS	101..952
Translation	MASLGQIIFWSIINIIIILAGAIALIIGFGISGKHFITVTTFTSAGNIGEDGTLSCTFEPDIKLNGIVIQWLKEGIKGLVHEFKEGKDDLSQQHEMFRGRTAVFADQVVVGNASLRLKNVQLTDAGTYTCYIRTSKGKGNANLEYKTGAFSMPEINVDYNASSESLRCEAPRWFPQPTVAWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTDSEVKRRSQLQLLNSGPSPCVFSSAFVAGWALLSLSCCLMLR

Target ORF information:

RefSeq Version	NM_178594.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus V-set domain containing T cell activation inhibitor 1 (Vtcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178594.3

ORF Insert Sequence:

ATGGCTTCCT TGGGGCAGAT CATCTTTTGG AGTATTATTA ACATCATCAT CATCCTGGCT 
GGGGCCATCG CACTCATCAT TGGCTTTGGC ATTTCAGGCA AGCACTTCAT CACGGTCACG 
ACCTTCACCT CAGCTGGAAA CATTGGAGAG GACGGGACCC TGAGCTGCAC TTTTGAACCT 
GACATCAAAC TCAACGGCAT CGTCATCCAG TGGCTGAAAG AAGGCATCAA AGGTTTGGTC 
CACGAGTTCA AAGAAGGCAA AGACGACCTC TCACAGCAGC ATGAGATGTT CAGAGGCCGC 
ACAGCAGTGT TTGCTGATCA GGTGGTAGTT GGCAATGCTT CCCTGAGACT GAAAAACGTG 
CAGCTCACGG ATGCTGGCAC CTACACATGT TACATCCGCA CCTCAAAAGG CAAAGGGAAT 
GCAAACCTTG AGTATAAGAC CGGAGCCTTC AGTATGCCAG AGATAAATGT GGACTATAAT 
GCCAGTTCAG AGAGTTTACG CTGCGAGGCT CCTCGGTGGT TCCCCCAGCC CACAGTGGCC 
TGGGCATCTC AAGTCGACCA AGGAGCCAAT TTCTCAGAAG TCTCCAACAC CAGCTTTGAG 
TTGAACTCTG AGAATGTGAC CATGAAGGTC GTATCTGTGC TCTACAATGT CACAATCAAC 
AACACATACT CCTGTATGAT TGAAAACGAC ATTGCCAAAG CCACCGGGGA CATCAAAGTG 
ACAGATTCAG AGGTCAAAAG GCGAAGTCAG CTGCAGTTGC TGAACTCTGG GCCTTCCCCG 
TGTGTTTTTT CTTCTGCCTT TGTGGCTGGC TGGGCACTCC TATCTCTCTC CTGTTGCCTG 
ATGCTAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vtcn1 ( XM_030252620.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108480.1 Mus musculus V-set domain containing T cell activation inhibitor 1 (Vtcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu88226
Clone ID Related Accession (Same CDS sequence)	XM_030252620.1
Accession Version	XM_030252620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	V-set domain containing T-cell activation inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAG GCCGCACAGC AGTGTTTGCT GATCAGGTGG TAGTTGGCAA TGCTTCCCTG 
AGACTGAAAA ACGTGCAGCT CACGGATGCT GGCACCTACA CATGTTACAT CCGCACCTCA 
AAAGGCAAAG GGAATGCAAA CCTTGAGTAT AAGACCGGAG CCTTCAGTAT GCCAGAGATA 
AATGTGGACT ATAATGCCAG TTCAGAGAGT TTACGCTGCG AGGCTCCTCG GTGGTTCCCC 
CAGCCCACAG TGGCCTGGGC ATCTCAAGTC GACCAAGGAG CCAATTTCTC AGAAGTCTCC 
AACACCAGCT TTGAGTTGAA CTCTGAGAAT GTGACCATGA AGGTCGTATC TGTGCTCTAC 
AATGTCACAA TCAACAACAC ATACTCCTGT ATGATTGAAA ACGACATTGC CAAAGCCACC 
GGGGACATCA AAGTGACAGA TTCAGAGGTC AAAAGGCGAA GTCAGCTGCA GTTGCTGAAC 
TCTGGGCCTT CCCCGTGTGT TTTTTCTTCT GCCTTTGTGG CTGGCTGGGC ACTCCTATCT 
CTCTCCTGTT GCCTGATGCT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108480.1
CDS	329..895
Translation	MFRGRTAVFADQVVVGNASLRLKNVQLTDAGTYTCYIRTSKGKGNANLEYKTGAFSMPEINVDYNASSESLRCEAPRWFPQPTVAWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTDSEVKRRSQLQLLNSGPSPCVFSSAFVAGWALLSLSCCLMLR

Target ORF information:

RefSeq Version	XM_030252620.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus V-set domain containing T cell activation inhibitor 1 (Vtcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252620.1

ORF Insert Sequence:

ATGTTCAGAG GCCGCACAGC AGTGTTTGCT GATCAGGTGG TAGTTGGCAA TGCTTCCCTG 
AGACTGAAAA ACGTGCAGCT CACGGATGCT GGCACCTACA CATGTTACAT CCGCACCTCA 
AAAGGCAAAG GGAATGCAAA CCTTGAGTAT AAGACCGGAG CCTTCAGTAT GCCAGAGATA 
AATGTGGACT ATAATGCCAG TTCAGAGAGT TTACGCTGCG AGGCTCCTCG GTGGTTCCCC 
CAGCCCACAG TGGCCTGGGC ATCTCAAGTC GACCAAGGAG CCAATTTCTC AGAAGTCTCC 
AACACCAGCT TTGAGTTGAA CTCTGAGAAT GTGACCATGA AGGTCGTATC TGTGCTCTAC 
AATGTCACAA TCAACAACAC ATACTCCTGT ATGATTGAAA ACGACATTGC CAAAGCCACC 
GGGGACATCA AAGTGACAGA TTCAGAGGTC AAAAGGCGAA GTCAGCTGCA GTTGCTGAAC 
TCTGGGCCTT CCCCGTGTGT TTTTTCTTCT GCCTTTGTGG CTGGCTGGGC ACTCCTATCT 
CTCTCCTGTT GCCTGATGCT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

B7-H4 enhances the differentiation of murine leukemia-initiating cells via the PTEN/AKT/RCOR2/RUNX1 pathways.
Leukemia31(10)2260-2264(2017 Oct)
Xia F,Zhang Y,Xie L,Jiang H,Zeng H,Chen C,Liu L,He X,Hao X,Fang X,Liu X,Zhang F,Gu H,Wan J,Cheng Y,Zhang CC,Chen GQ,Lu Y,Yu Z,Zheng J

Synergistic effects of host B7-H4 deficiency and gemcitabine treatment on tumor regression and anti-tumor T cell immunity in a mouse model.
Cancer immunology, immunotherapy : CII66(4)491-502(2017 Apr)
Leung J,St-Onge P,Stagg J,Suh WK

Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.
Journal of immunology (Baltimore, Md. : 1950)196(4)1495-506(2016 Feb)
Radichev IA,Maneva-Radicheva LV,Amatya C,Salehi M,Parker C,Ellefson J,Burn P,Savinov AY

Ablation of B7-H3 but Not B7-H4 Results in Highly Increased Tumor Burden in a Murine Model of Spontaneous Prostate Cancer.
Cancer immunology research3(8)849-54(2015 Aug)
Kreymborg K,Haak S,Murali R,Wei J,Waitz R,Gasteiger G,Savage PA,van den Brink MR,Allison JP