Tmf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmf1
Entrez Gene ID	232286
Full Name	TATA element modulatory factor 1
Synonyms	7030402D04Rik,Gm153,Tmf1`
General protein information	Preferred Names TATA element modulatory factor 1 Names TATA element modulatory factor TMF androgen receptor coactivator 160 kDa protein androgen receptor-associated protein of 160 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030255349.1	XP_030111209.1	TATA element modulatory factor isoform X1
NM_001081111.2	NP_001074580.1	TATA element modulatory factor

Pathways from BioSystems

Homologies

	TMF1	XP_001088711.2
Danio rerio (zebrafish)	tmf1	NP_001003522.1
Canis lupus familiaris (dog)	TMF1	XP_005632331.1
Homo sapiens (human)	TMF1	NP_009045.2
Bos taurus (cattle)	TMF1	NP_001193189.1
Pan troglodytes (chimpanzee)	TMF1	XP_526227.2
Mus musculus (house mouse)	Tmf1	NP_001074580.1
Rattus norvegicus (Norway rat)	Tmf1	NP_446123.2
Gallus gallus (chicken)	TMF1	XP_423749.2

Gene Ontology
Bibliography

Related articles in PubMed

TMF/ARA160 Governs the Dynamic Spatial Orientation of the Golgi Apparatus during Sperm Development.
Elkis Y, Bel S, Rahimi R, Lerer-Goldstein T, Levin-Zaidman S, Babushkin T, Shpungin S, Nir U
PloS one10(12)e0145277(2015)

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF-/- mice.
Bel S, Elkis Y, Elifantz H, Koren O, Ben-Hamo R, Lerer-Goldshtein T, Rahimi R, Ben Horin S, Nyska A, Shpungin S, Nir U
Proceedings of the National Academy of Sciences of the United States of America111(13)4964-9(2014 Apr)

Testosterone deficiency accompanied by testicular and epididymal abnormalities in TMF(-/-) mice.
Elkis Y, Bel S, Lerer-Goldstein T, Nyska A, Creasy DM, Shpungin S, Nir U
Molecular and cellular endocrinology365(1)52-63(2013 Jan)

Loss of TMF/ARA160 protein renders colonic mucus refractory to bacterial colonization and diminishes intestinal susceptibility to acute colitis.
Bel S, Elkis Y, Lerer-Goldstein T, Nyska A, Shpungin S, Nir U
The Journal of biological chemistry287(30)25631-9(2012 Jul)

CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion.
Gu P, Min JN, Wang Y, Huang C, Peng T, Chai W, Chang S
The EMBO journal31(10)2309-21(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The microtubule interacting domain (MIT) domain in TMF, along with microtubule integrity, are required for stable association of TMF with the Golgi apparatus.
Title: TMF/ARA160 Governs the Dynamic Spatial Orientation of the Golgi Apparatus during Sperm Development.

Altered mucus secretion in TMF(-/-) mouse colons is accompanied by a reprogrammed intestinal microbiota, leading to a transmissible reduced sensitivity to induced colitis.
Title: Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF-/- mice.

Data from knockout mice suggest that TMF/ARA160 participates in control of serum testosterone levels in males, and its absence results in major testicular and epididymal defects and abnormal spermatogenesis and Leydig cell proliferation.
Title: Testosterone deficiency accompanied by testicular and epididymal abnormalities in TMF(-/-) mice.

absence of TMF/ARA160 renders the colonic mucus refractory to bacterial colonization and the large intestine less susceptible to the onset of colitis.
Title: Loss of TMF/ARA160 protein renders colonic mucus refractory to bacterial colonization and diminishes intestinal susceptibility to acute colitis.

TMF/ARA160 is a key regulator which is essential for the onset of key events in the differentiation and maturation of mammalian sperm and whose absence severely compromises their ability to fertilize ova.
Title: TMF/ARA160: A key regulator of sperm development.

The following Tmf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu89188	XM_030255349.1 Latest version!	Mus musculus TATA element modulatory factor 1 (Tmf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu19620	NM_001081111.2 Latest version!	Mus musculus TATA element modulatory factor 1 (Tmf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmf1 ( XM_030255349.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111209.1 Mus musculus TATA element modulatory factor 1 (Tmf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89188
Clone ID Related Accession (Same CDS sequence)	XM_030255349.1
Accession Version	XM_030255349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	TATA element modulatory factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGG AACCTAAAGA ACAAAAACAC GAAGACAGAC AGAGCAACAC ACCTTCTCCT 
CCTGTTAGCT CCTTCTCGTC GGGCACCTCC ACCACTAGTG ACATTGAAGT TTTAGATCAC 
GAGAGTGTAA TCAGTGAGAG CTCGGCCAGT TCGAGGCAAG AGACTTCGGA TGCAAAGTCG 
AGTCTTCACC TCATGCAGAC GTCATTTCAG CTCCTCTCTG CGTCTGCTTG TCCCGAGTAT 
AGCCGCTTAG ATGACTTCCA GAAGCTCAAT GAGAGTTGCT GCTCATCGGA TGCTTTTGAA 
AGAATAGACT CATTTAGCGT GCAGTCTCTA GATAGCCGGA GCGTAAGTGA AATCAACTCA 
GACGACGAAC TGCCAGGCAA AGGCTATGCT TTAGTACCCA TAATAGTGAG TCCTTCAACC 
CCAAAGACTA AAGTAGTAGA ATCTACTGAA GAGAATGCTG AAGAAGAGGA AGGAAACGAG 
ACATTAGTTG CGCCGTCTGA GGAAGCAGAA TTAGAGGAAA GTGGCCGAAG TGCTACTCCA 
GTGAACTGTG ACCAGCCAGA TATATTGGCT TCTCCCACAG CAGGAAGTGG CGGGCACTCT 
GCCTCTGGAC CGGCCACTGA GCAGTGTGAA GCTGTTGAAA ACCAGCCAAA AGCACCGCCA 
GAGAAAGAAG ATGTTTGCAA GACAGTTGAA TTTCTGAATG AGAAGCTAGA AAAGAGGGAG 
ACTCAGTTAT TGTCCCTTAG TAAAGAAAAG GCACTTCTGG AGGAAGCGTA TGATAACCTG 
AAGGATGAAA TGTTCAGAGT AAAAGAAGAA AGTAGTAGCA TTTCTTCCCT GAAAGATGAA 
TTTACCCAGA GAATTGCAGA AGCAGAAAAG AAAGTTCAAC TAGCCTGCAA GGAGAGAGAT 
GCTGCTAAGA AGGAAATGAA AACCATAAAG GAAGAACTTG CCACTAGATT AAATAGTAGT 
CAAACTGCAG ACCTGTTGAA GGAAAAAGAT GAACAGATTC AAGGTTTAAT GGAAGAAGGA 
GAAAAACTTT CAAAGCAGCA GCTGCATAAT TCCAATATCA TTAAGAAATT AAGAGCTAAA 
GATAAAGACA ATGAAAATGT AATTGCAAAG CTGAACAGAA AAGCTAAGGA GCTGGAAGAG 
GAGTTACAGC ACTTAAGACA GGTCCTTGAT GGCAAAGAGG AGGTTGAGAA ACAACATAGA 
GAAAATATTA AAAAACTAAA TTCTGTGGTA GAACGCCAGG AGAAAGATCT TGGCCGACTT 
CAGGTAGACA TGGATGAACT TGAAGAGAAG AGCCGAAGTA CTCAGGCTGC CCTAGATAGT 
GCGTACAGAG AACTTACCGA TCTTCACAAA GCCAATGCTG CTAAGGACAG TGAGGTACAG 
GAAGCTGCTC TGAGGCGTGA GATGAAGGCT AAAGAAGAAC TTTCTGGGGC ACTGGAGAAG 
GCCCAGGAAG AAGCCCGTCA GCAGCAAGAG GCATTAGTGC TTCAGGTGGG GGACCTCAGG 
CTAGCGCTGC AGCGGGCGGA GCAAGCAGCT GCCAGAAAAG AGGATTACTT ACGCCATGAG 
ATCAGTGAAC TTCAGCAGAG ACTCCAAGAA GCAGAGAATC GAAACCAAGA GCTGAGTCAA 
AGTGTTTCAT CAACAGCCAG ACCGTTGCTG CGACAGATAG AAAACTTACA AGCAACTCTG 
GGGTCCCAGA CGTCCTCGTG GGAGACACTG GAGAAGAGTC TTTCTGATAG GCTTGGTGAA 
TCACAGACCT TGTTGGCTGC AGCAGTTGAA AGAGAACGTG CAGCTACAGA AGAACTCCTG 
GCCAACAAAA TCCAGATGTC TTCAGTGGAG TCACAGAATA CGTTATTACG ACAGGAAAAC 
AGTAGACTTC AGGCCCAGCT AGAGTCAGAG AAAAATAAAC TAAGAAAACT AGAGGATGAA 
AATAGTCGGT ACCAAGTTGA ATTAGAAAAC CTAAAAGATG AATATGTAAG AACGCTTGAA 
GAGTCAAGGA AAGAGAAGAC ACTTTTGAGC AGTCAGTTAG AAATGGAAAG AATGAAAGTT 
GAACAAGAAA GGAAGAAAAC CATTTTCACT CAAGAAGCAC TAAAAGAAAA GGACCACAAG 
CTGTTTTCTG TTTGTAGCAC TCCCACCATG TCACGTTCCA GCTCTATAAG TGGAGTCGAT 
GCTGCAGGGC TGCAAGCGTC CTTCCTGTCT CAGGATGAGT CTCATGACCA TTCATTTGGG 
CCGATGTCTA CATCAGCCAG TGGAAGCAAC CTTTATGAAG CTGTAAGGAT GGGAGCTGGA 
TCGAGCATTA TTGAAAATCT GCAGTCTCAG CTAAAGCTGA GGGAAGGAGA AATTAGTCAT 
TTACAGCTGG AAATTAGTAA CCTAGAAAAA ACTCGATCCA TAATGTCCGA AGAGCTAGTT 
AAATTAACAA ATCAGAATGA TGAACTTGAG GAGAAAGTGA AAGAGATACC CAAGCTTCGA 
GTTCAGCTGC GAGATTTGGA TCAAAGATAC AACACTATTC TGCAGATGTA TGGAGAAAAA 
GCAGAAGAAG CAGAGGAACT TCGCTTGGAT CTTGAAGATG TAAAAAATAT GTATAAAACT 
CAAATAGATG AACTTCTAAG ACAAAGGCTC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111209.1
CDS	1160..3775
Translation	MALEPKEQKHEDRQSNTPSPPVSSFSSGTSTTSDIEVLDHESVISESSASSRQETSDAKSSLHLMQTSFQLLSASACPEYSRLDDFQKLNESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELPGKGYALVPIIVSPSTPKTKVVESTEENAEEEEGNETLVAPSEEAELEESGRSATPVNCDQPDILASPTAGSGGHSASGPATEQCEAVENQPKAPPEKEDVCKTVEFLNEKLEKRETQLLSLSKEKALLEEAYDNLKDEMFRVKEESSSISSLKDEFTQRIAEAEKKVQLACKERDAAKKEMKTIKEELATRLNSSQTADLLKEKDEQIQGLMEEGEKLSKQQLHNSNIIKKLRAKDKDNENVIAKLNRKAKELEEELQHLRQVLDGKEEVEKQHRENIKKLNSVVERQEKDLGRLQVDMDELEEKSRSTQAALDSAYRELTDLHKANAAKDSEVQEAALRREMKAKEELSGALEKAQEEARQQQEALVLQVGDLRLALQRAEQAAARKEDYLRHEISELQQRLQEAENRNQELSQSVSSTARPLLRQIENLQATLGSQTSSWETLEKSLSDRLGESQTLLAAAVERERAATEELLANKIQMSSVESQNTLLRQENSRLQAQLESEKNKLRKLEDENSRYQVELENLKDEYVRTLEESRKEKTLLSSQLEMERMKVEQERKKTIFTQEALKEKDHKLFSVCSTPTMSRSSSISGVDAAGLQASFLSQDESHDHSFGPMSTSASGSNLYEAVRMGAGSSIIENLQSQLKLREGEISHLQLEISNLEKTRSIMSEELVKLTNQNDELEEKVKEIPKLRVQLRDLDQRYNTILQMYGEKAEEAEELRLDLEDVKNMYKTQIDELLRQRLS

Target ORF information:

RefSeq Version	XM_030255349.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TATA element modulatory factor 1 (Tmf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255349.1

ORF Insert Sequence:

ATGGCTTTGG AACCTAAAGA ACAAAAACAC GAAGACAGAC AGAGCAACAC ACCTTCTCCT 
CCTGTTAGCT CCTTCTCGTC GGGCACCTCC ACCACTAGTG ACATTGAAGT TTTAGATCAC 
GAGAGTGTAA TCAGTGAGAG CTCGGCCAGT TCGAGGCAAG AGACTTCGGA TGCAAAGTCG 
AGTCTTCACC TCATGCAGAC GTCATTTCAG CTCCTCTCTG CGTCTGCTTG TCCCGAGTAT 
AGCCGCTTAG ATGACTTCCA GAAGCTCAAT GAGAGTTGCT GCTCATCGGA TGCTTTTGAA 
AGAATAGACT CATTTAGCGT GCAGTCTCTA GATAGCCGGA GCGTAAGTGA AATCAACTCA 
GACGACGAAC TGCCAGGCAA AGGCTATGCT TTAGTACCCA TAATAGTGAG TCCTTCAACC 
CCAAAGACTA AAGTAGTAGA ATCTACTGAA GAGAATGCTG AAGAAGAGGA AGGAAACGAG 
ACATTAGTTG CGCCGTCTGA GGAAGCAGAA TTAGAGGAAA GTGGCCGAAG TGCTACTCCA 
GTGAACTGTG ACCAGCCAGA TATATTGGCT TCTCCCACAG CAGGAAGTGG CGGGCACTCT 
GCCTCTGGAC CGGCCACTGA GCAGTGTGAA GCTGTTGAAA ACCAGCCAAA AGCACCGCCA 
GAGAAAGAAG ATGTTTGCAA GACAGTTGAA TTTCTGAATG AGAAGCTAGA AAAGAGGGAG 
ACTCAGTTAT TGTCCCTTAG TAAAGAAAAG GCACTTCTGG AGGAAGCGTA TGATAACCTG 
AAGGATGAAA TGTTCAGAGT AAAAGAAGAA AGTAGTAGCA TTTCTTCCCT GAAAGATGAA 
TTTACCCAGA GAATTGCAGA AGCAGAAAAG AAAGTTCAAC TAGCCTGCAA GGAGAGAGAT 
GCTGCTAAGA AGGAAATGAA AACCATAAAG GAAGAACTTG CCACTAGATT AAATAGTAGT 
CAAACTGCAG ACCTGTTGAA GGAAAAAGAT GAACAGATTC AAGGTTTAAT GGAAGAAGGA 
GAAAAACTTT CAAAGCAGCA GCTGCATAAT TCCAATATCA TTAAGAAATT AAGAGCTAAA 
GATAAAGACA ATGAAAATGT AATTGCAAAG CTGAACAGAA AAGCTAAGGA GCTGGAAGAG 
GAGTTACAGC ACTTAAGACA GGTCCTTGAT GGCAAAGAGG AGGTTGAGAA ACAACATAGA 
GAAAATATTA AAAAACTAAA TTCTGTGGTA GAACGCCAGG AGAAAGATCT TGGCCGACTT 
CAGGTAGACA TGGATGAACT TGAAGAGAAG AGCCGAAGTA CTCAGGCTGC CCTAGATAGT 
GCGTACAGAG AACTTACCGA TCTTCACAAA GCCAATGCTG CTAAGGACAG TGAGGTACAG 
GAAGCTGCTC TGAGGCGTGA GATGAAGGCT AAAGAAGAAC TTTCTGGGGC ACTGGAGAAG 
GCCCAGGAAG AAGCCCGTCA GCAGCAAGAG GCATTAGTGC TTCAGGTGGG GGACCTCAGG 
CTAGCGCTGC AGCGGGCGGA GCAAGCAGCT GCCAGAAAAG AGGATTACTT ACGCCATGAG 
ATCAGTGAAC TTCAGCAGAG ACTCCAAGAA GCAGAGAATC GAAACCAAGA GCTGAGTCAA 
AGTGTTTCAT CAACAGCCAG ACCGTTGCTG CGACAGATAG AAAACTTACA AGCAACTCTG 
GGGTCCCAGA CGTCCTCGTG GGAGACACTG GAGAAGAGTC TTTCTGATAG GCTTGGTGAA 
TCACAGACCT TGTTGGCTGC AGCAGTTGAA AGAGAACGTG CAGCTACAGA AGAACTCCTG 
GCCAACAAAA TCCAGATGTC TTCAGTGGAG TCACAGAATA CGTTATTACG ACAGGAAAAC 
AGTAGACTTC AGGCCCAGCT AGAGTCAGAG AAAAATAAAC TAAGAAAACT AGAGGATGAA 
AATAGTCGGT ACCAAGTTGA ATTAGAAAAC CTAAAAGATG AATATGTAAG AACGCTTGAA 
GAGTCAAGGA AAGAGAAGAC ACTTTTGAGC AGTCAGTTAG AAATGGAAAG AATGAAAGTT 
GAACAAGAAA GGAAGAAAAC CATTTTCACT CAAGAAGCAC TAAAAGAAAA GGACCACAAG 
CTGTTTTCTG TTTGTAGCAC TCCCACCATG TCACGTTCCA GCTCTATAAG TGGAGTCGAT 
GCTGCAGGGC TGCAAGCGTC CTTCCTGTCT CAGGATGAGT CTCATGACCA TTCATTTGGG 
CCGATGTCTA CATCAGCCAG TGGAAGCAAC CTTTATGAAG CTGTAAGGAT GGGAGCTGGA 
TCGAGCATTA TTGAAAATCT GCAGTCTCAG CTAAAGCTGA GGGAAGGAGA AATTAGTCAT 
TTACAGCTGG AAATTAGTAA CCTAGAAAAA ACTCGATCCA TAATGTCCGA AGAGCTAGTT 
AAATTAACAA ATCAGAATGA TGAACTTGAG GAGAAAGTGA AAGAGATACC CAAGCTTCGA 
GTTCAGCTGC GAGATTTGGA TCAAAGATAC AACACTATTC TGCAGATGTA TGGAGAAAAA 
GCAGAAGAAG CAGAGGAACT TCGCTTGGAT CTTGAAGATG TAAAAAATAT GTATAAAACT 
CAAATAGATG AACTTCTAAG ACAAAGGCTC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmf1 ( NM_001081111.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074580.1 Mus musculus TATA element modulatory factor 1 (Tmf1), mRNA.

Price & Availability↑

CloneID	OMu19620
Clone ID Related Accession (Same CDS sequence)	NM_001081111.2
Accession Version	NM_001081111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	TATA element modulatory factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC155724.8, AK144898.1, CN456538.1, BQ561257.1, BE627586.1 and BQ561256.1. On Jan 13, 2010 this sequence version replaced NM_001081111.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC150931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAGG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGCGAGCC GGGAATAAGT CCCCCTGTGA GTGGAGGATG GGACACTTCA 
ACCTGGGGAT TGAACTCAAC AAGCAGCGAA CCTCAGAGTC CCCCAACAGC CTCTCAAGCA 
ATTACAAAGC CAGTTCGAAG GACTGTAGTA GATGAATCTG AAAATTTCTT CAGTGCCTTT 
CTCTCTCCGT CGGATGCCCA TACCATTCAG AAGAGCCCAG TGGTATCAAA ACCTCCATCT 
AAATCCCAGC GGCCAGAGGA AGAAGTGAAA AGCAGCTTAC AAGAATCGTC ATCTCCTGGC 
CAGTCCAGAG TTTCTGAGAC AGCTGAAGTG AGAGACTCCG TGTGTGTTTC TGGAGAGACT 
TCAGCCGTGG GCACTCCCTC ACCTGTACCT GAAGACAAGC ATGAAGAAAC GGCAGGTGAA 
GAGTCTGAGG TGAAGGTGCC CACTGTGAGA TTGAAGGCGT CTGAAAACGT AGTTAATGTA 
AACACAACAG AAGATGTATC TACCACATCC ACTCAGTCTC TCACGGCAGA AACGAAGGAT 
ATGGCTTTGG AACCTAAAGA ACAAAAACAC GAAGACAGAC AGAGCAACAC ACCTTCTCCT 
CCTGTTAGCT CCTTCTCGTC GGGCACCTCC ACCACTAGTG ACATTGAAGT TTTAGATCAC 
GAGAGTGTAA TCAGTGAGAG CTCGGCCAGT TCGAGGCAAG AGACTTCGGA TGCAAAGTCG 
AGTCTTCACC TCATGCAGAC GTCATTTCAG CTCCTCTCTG CGTCTGCTTG TCCCGAGTAT 
AGCCGCTTAG ATGACTTCCA GAAGCTCAAT GAGAGTTGCT GCTCATCGGA TGCTTTTGAA 
AGAATAGACT CATTTAGCGT GCAGTCTCTA GATAGCCGGA GCGTAAGTGA AATCAACTCA 
GACGACGAAC TGCCAGGCAA AGGCTATGCT TTAGTACCCA TAATAGTGAG TCCTTCAACC 
CCAAAGACTA AAGTAGTAGA ATCTACTGAA GAGAATGCTG AAGAAGAGGA AGGAAACGAG 
ACATTAGTTG CGCCGTCTGA GGAAGCAGAA TTAGAGGAAA GTGGCCGAAG TGCTACTCCA 
GTGAACTGTG ACCAGCCAGA TATATTGGCT TCTCCCACAG CAGGAAGTGG CGGGCACTCT 
GCCTCTGGAC CGGCCACTGA GCAGTGTGAA GCTGTTGAAA ACCAGCCAAA AGCACCGCCA 
GAGAAAGAAG ATGTTTGCAA GACAGTTGAA TTTCTGAATG AGAAGCTAGA AAAGAGGGAG 
ACTCAGTTAT TGTCCCTTAG TAAAGAAAAG GCACTTCTGG AGGAAGCGTA TGATAACCTG 
AAGGATGAAA TGTTCAGAGT AAAAGAAGAA AGTAGTAGCA TTTCTTCCCT GAAAGATGAA 
TTTACCCAGA GAATTGCAGA AGCAGAAAAG AAAGTTCAAC TAGCCTGCAA GGAGAGAGAT 
GCTGCTAAGA AGGAAATGAA AACCATAAAG GAAGAACTTG CCACTAGATT AAATAGTAGT 
CAAACTGCAG ACCTGTTGAA GGAAAAAGAT GAACAGATTC AAGGTTTAAT GGAAGAAGGA 
GAAAAACTTT CAAAGCAGCA GCTGCATAAT TCCAATATCA TTAAGAAATT AAGAGCTAAA 
GATAAAGACA ATGAAAATGT AATTGCAAAG CTGAACAGAA AAGCTAAGGA GCTGGAAGAG 
GAGTTACAGC ACTTAAGACA GGTCCTTGAT GGCAAAGAGG AGGTTGAGAA ACAACATAGA 
GAAAATATTA AAAAACTAAA TTCTGTGGTA GAACGCCAGG AGAAAGATCT TGGCCGACTT 
CAGGTAGACA TGGATGAACT TGAAGAGAAG AGCCGAAGTA CTCAGGCTGC CCTAGATAGT 
GCGTACAGAG AACTTACCGA TCTTCACAAA GCCAATGCTG CTAAGGACAG TGAGGTACAG 
GAAGCTGCTC TGAGGCGTGA GATGAAGGCT AAAGAAGAAC TTTCTGGGGC ACTGGAGAAG 
GCCCAGGAAG AAGCCCGTCA GCAGCAAGAG GCATTAGTGC TTCAGGTGGG GGACCTCAGG 
CTAGCGCTGC AGCGGGCGGA GCAAGCAGCT GCCAGAAAAG AGGATTACTT ACGCCATGAG 
ATCAGTGAAC TTCAGCAGAG ACTCCAAGAA GCAGAGAATC GAAACCAAGA GCTGAGTCAA 
AGTGTTTCAT CAACAGCCAG ACCGTTGCTG CGACAGATAG AAAACTTACA AGCAACTCTG 
GGGTCCCAGA CGTCCTCGTG GGAGACACTG GAGAAGAGTC TTTCTGATAG GCTTGGTGAA 
TCACAGACCT TGTTGGCTGC AGCAGTTGAA AGAGAACGTG CAGCTACAGA AGAACTCCTG 
GCCAACAAAA TCCAGATGTC TTCAGTGGAG TCACAGAATA CGTTATTACG ACAGGAAAAC 
AGTAGACTTC AGGCCCAGCT AGAGTCAGAG AAAAATAAAC TAAGAAAACT AGAGGATGAA 
AATAGTCGGT ACCAAGTTGA ATTAGAAAAC CTAAAAGATG AATATGTAAG AACGCTTGAA 
GAGTCAAGGA AAGAGAAGAC ACTTTTGAGC AGTCAGTTAG AAATGGAAAG AATGAAAGTT 
GAACAAGAAA GGAAGAAAAC CATTTTCACT CAAGAAGCAC TAAAAGAAAA GGACCACAAG 
CTGTTTTCTG TTTGTAGCAC TCCCACCATG TCACGTTCCA GCTCTATAAG TGGAGTCGAT 
GCTGCAGGGC TGCAAGCGTC CTTCCTGTCT CAGGATGAGT CTCATGACCA TTCATTTGGG 
CCGATGTCTA CATCAGCCAG TGGAAGCAAC CTTTATGAAG CTGTAAGGAT GGGAGCTGGA 
TCGAGCATTA TTGAAAATCT GCAGTCTCAG CTAAAGCTGA GGGAAGGAGA AATTAGTCAT 
TTACAGCTGG AAATTAGTAA CCTAGAAAAA ACTCGATCCA TAATGTCCGA AGAGCTAGTT 
AAATTAACAA ATCAGAATGA TGAACTTGAG GAGAAAGTGA AAGAGATACC CAAGCTTCGA 
GTTCAGCTGC GAGATTTGGA TCAAAGATAC AACACTATTC TGCAGATGTA TGGAGAAAAA 
GCAGAAGAAG CAGAGGAACT TCGCTTGGAT CTTGAAGATG TAAAAAATAT GTATAAAACT 
CAAATAGATG AACTTCTAAG ACAAAGGCTC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074580.1
CDS	102..3377
Translation	MSWFNASQLSSFAKQALSQAQKSIDRVLDIQEEEPSAWAEAIPYGEPGISPPVSGGWDTSTWGLNSTSSEPQSPPTASQAITKPVRRTVVDESENFFSAFLSPSDAHTIQKSPVVSKPPSKSQRPEEEVKSSLQESSSPGQSRVSETAEVRDSVCVSGETSAVGTPSPVPEDKHEETAGEESEVKVPTVRLKASENVVNVNTTEDVSTTSTQSLTAETKDMALEPKEQKHEDRQSNTPSPPVSSFSSGTSTTSDIEVLDHESVISESSASSRQETSDAKSSLHLMQTSFQLLSASACPEYSRLDDFQKLNESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELPGKGYALVPIIVSPSTPKTKVVESTEENAEEEEGNETLVAPSEEAELEESGRSATPVNCDQPDILASPTAGSGGHSASGPATEQCEAVENQPKAPPEKEDVCKTVEFLNEKLEKRETQLLSLSKEKALLEEAYDNLKDEMFRVKEESSSISSLKDEFTQRIAEAEKKVQLACKERDAAKKEMKTIKEELATRLNSSQTADLLKEKDEQIQGLMEEGEKLSKQQLHNSNIIKKLRAKDKDNENVIAKLNRKAKELEEELQHLRQVLDGKEEVEKQHRENIKKLNSVVERQEKDLGRLQVDMDELEEKSRSTQAALDSAYRELTDLHKANAAKDSEVQEAALRREMKAKEELSGALEKAQEEARQQQEALVLQVGDLRLALQRAEQAAARKEDYLRHEISELQQRLQEAENRNQELSQSVSSTARPLLRQIENLQATLGSQTSSWETLEKSLSDRLGESQTLLAAAVERERAATEELLANKIQMSSVESQNTLLRQENSRLQAQLESEKNKLRKLEDENSRYQVELENLKDEYVRTLEESRKEKTLLSSQLEMERMKVEQERKKTIFTQEALKEKDHKLFSVCSTPTMSRSSSISGVDAAGLQASFLSQDESHDHSFGPMSTSASGSNLYEAVRMGAGSSIIENLQSQLKLREGEISHLQLEISNLEKTRSIMSEELVKLTNQNDELEEKVKEIPKLRVQLRDLDQRYNTILQMYGEKAEEAEELRLDLEDVKNMYKTQIDELLRQRLS

Target ORF information:

RefSeq Version	NM_001081111.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TATA element modulatory factor 1 (Tmf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081111.2

ORF Insert Sequence:

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAGG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGCGAGCC GGGAATAAGT CCCCCTGTGA GTGGAGGATG GGACACTTCA 
ACCTGGGGAT TGAACTCAAC AAGCAGCGAA CCTCAGAGTC CCCCAACAGC CTCTCAAGCA 
ATTACAAAGC CAGTTCGAAG GACTGTAGTA GATGAATCTG AAAATTTCTT CAGTGCCTTT 
CTCTCTCCGT CGGATGCCCA TACCATTCAG AAGAGCCCAG TGGTATCAAA ACCTCCATCT 
AAATCCCAGC GGCCAGAGGA AGAAGTGAAA AGCAGCTTAC AAGAATCGTC ATCTCCTGGC 
CAGTCCAGAG TTTCTGAGAC AGCTGAAGTG AGAGACTCCG TGTGTGTTTC TGGAGAGACT 
TCAGCCGTGG GCACTCCCTC ACCTGTACCT GAAGACAAGC ATGAAGAAAC GGCAGGTGAA 
GAGTCTGAGG TGAAGGTGCC CACTGTGAGA TTGAAGGCGT CTGAAAACGT AGTTAATGTA 
AACACAACAG AAGATGTATC TACCACATCC ACTCAGTCTC TCACGGCAGA AACGAAGGAT 
ATGGCTTTGG AACCTAAAGA ACAAAAACAC GAAGACAGAC AGAGCAACAC ACCTTCTCCT 
CCTGTTAGCT CCTTCTCGTC GGGCACCTCC ACCACTAGTG ACATTGAAGT TTTAGATCAC 
GAGAGTGTAA TCAGTGAGAG CTCGGCCAGT TCGAGGCAAG AGACTTCGGA TGCAAAGTCG 
AGTCTTCACC TCATGCAGAC GTCATTTCAG CTCCTCTCTG CGTCTGCTTG TCCCGAGTAT 
AGCCGCTTAG ATGACTTCCA GAAGCTCAAT GAGAGTTGCT GCTCATCGGA TGCTTTTGAA 
AGAATAGACT CATTTAGCGT GCAGTCTCTA GATAGCCGGA GCGTAAGTGA AATCAACTCA 
GACGACGAAC TGCCAGGCAA AGGCTATGCT TTAGTACCCA TAATAGTGAG TCCTTCAACC 
CCAAAGACTA AAGTAGTAGA ATCTACTGAA GAGAATGCTG AAGAAGAGGA AGGAAACGAG 
ACATTAGTTG CGCCGTCTGA GGAAGCAGAA TTAGAGGAAA GTGGCCGAAG TGCTACTCCA 
GTGAACTGTG ACCAGCCAGA TATATTGGCT TCTCCCACAG CAGGAAGTGG CGGGCACTCT 
GCCTCTGGAC CGGCCACTGA GCAGTGTGAA GCTGTTGAAA ACCAGCCAAA AGCACCGCCA 
GAGAAAGAAG ATGTTTGCAA GACAGTTGAA TTTCTGAATG AGAAGCTAGA AAAGAGGGAG 
ACTCAGTTAT TGTCCCTTAG TAAAGAAAAG GCACTTCTGG AGGAAGCGTA TGATAACCTG 
AAGGATGAAA TGTTCAGAGT AAAAGAAGAA AGTAGTAGCA TTTCTTCCCT GAAAGATGAA 
TTTACCCAGA GAATTGCAGA AGCAGAAAAG AAAGTTCAAC TAGCCTGCAA GGAGAGAGAT 
GCTGCTAAGA AGGAAATGAA AACCATAAAG GAAGAACTTG CCACTAGATT AAATAGTAGT 
CAAACTGCAG ACCTGTTGAA GGAAAAAGAT GAACAGATTC AAGGTTTAAT GGAAGAAGGA 
GAAAAACTTT CAAAGCAGCA GCTGCATAAT TCCAATATCA TTAAGAAATT AAGAGCTAAA 
GATAAAGACA ATGAAAATGT AATTGCAAAG CTGAACAGAA AAGCTAAGGA GCTGGAAGAG 
GAGTTACAGC ACTTAAGACA GGTCCTTGAT GGCAAAGAGG AGGTTGAGAA ACAACATAGA 
GAAAATATTA AAAAACTAAA TTCTGTGGTA GAACGCCAGG AGAAAGATCT TGGCCGACTT 
CAGGTAGACA TGGATGAACT TGAAGAGAAG AGCCGAAGTA CTCAGGCTGC CCTAGATAGT 
GCGTACAGAG AACTTACCGA TCTTCACAAA GCCAATGCTG CTAAGGACAG TGAGGTACAG 
GAAGCTGCTC TGAGGCGTGA GATGAAGGCT AAAGAAGAAC TTTCTGGGGC ACTGGAGAAG 
GCCCAGGAAG AAGCCCGTCA GCAGCAAGAG GCATTAGTGC TTCAGGTGGG GGACCTCAGG 
CTAGCGCTGC AGCGGGCGGA GCAAGCAGCT GCCAGAAAAG AGGATTACTT ACGCCATGAG 
ATCAGTGAAC TTCAGCAGAG ACTCCAAGAA GCAGAGAATC GAAACCAAGA GCTGAGTCAA 
AGTGTTTCAT CAACAGCCAG ACCGTTGCTG CGACAGATAG AAAACTTACA AGCAACTCTG 
GGGTCCCAGA CGTCCTCGTG GGAGACACTG GAGAAGAGTC TTTCTGATAG GCTTGGTGAA 
TCACAGACCT TGTTGGCTGC AGCAGTTGAA AGAGAACGTG CAGCTACAGA AGAACTCCTG 
GCCAACAAAA TCCAGATGTC TTCAGTGGAG TCACAGAATA CGTTATTACG ACAGGAAAAC 
AGTAGACTTC AGGCCCAGCT AGAGTCAGAG AAAAATAAAC TAAGAAAACT AGAGGATGAA 
AATAGTCGGT ACCAAGTTGA ATTAGAAAAC CTAAAAGATG AATATGTAAG AACGCTTGAA 
GAGTCAAGGA AAGAGAAGAC ACTTTTGAGC AGTCAGTTAG AAATGGAAAG AATGAAAGTT 
GAACAAGAAA GGAAGAAAAC CATTTTCACT CAAGAAGCAC TAAAAGAAAA GGACCACAAG 
CTGTTTTCTG TTTGTAGCAC TCCCACCATG TCACGTTCCA GCTCTATAAG TGGAGTCGAT 
GCTGCAGGGC TGCAAGCGTC CTTCCTGTCT CAGGATGAGT CTCATGACCA TTCATTTGGG 
CCGATGTCTA CATCAGCCAG TGGAAGCAAC CTTTATGAAG CTGTAAGGAT GGGAGCTGGA 
TCGAGCATTA TTGAAAATCT GCAGTCTCAG CTAAAGCTGA GGGAAGGAGA AATTAGTCAT 
TTACAGCTGG AAATTAGTAA CCTAGAAAAA ACTCGATCCA TAATGTCCGA AGAGCTAGTT 
AAATTAACAA ATCAGAATGA TGAACTTGAG GAGAAAGTGA AAGAGATACC CAAGCTTCGA 
GTTCAGCTGC GAGATTTGGA TCAAAGATAC AACACTATTC TGCAGATGTA TGGAGAAAAA 
GCAGAAGAAG CAGAGGAACT TCGCTTGGAT CTTGAAGATG TAAAAAATAT GTATAAAACT 
CAAATAGATG AACTTCTAAG ACAAAGGCTC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TMF/ARA160 Governs the Dynamic Spatial Orientation of the Golgi Apparatus during Sperm Development.
PloS one10(12)e0145277(2015)
Elkis Y,Bel S,Rahimi R,Lerer-Goldstein T,Levin-Zaidman S,Babushkin T,Shpungin S,Nir U

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF-/- mice.
Proceedings of the National Academy of Sciences of the United States of America111(13)4964-9(2014 Apr)
Bel S,Elkis Y,Elifantz H,Koren O,Ben-Hamo R,Lerer-Goldshtein T,Rahimi R,Ben Horin S,Nyska A,Shpungin S,Nir U

Testosterone deficiency accompanied by testicular and epididymal abnormalities in TMF(-/-) mice.
Molecular and cellular endocrinology365(1)52-63(2013 Jan)
Elkis Y,Bel S,Lerer-Goldstein T,Nyska A,Creasy DM,Shpungin S,Nir U

Loss of TMF/ARA160 protein renders colonic mucus refractory to bacterial colonization and diminishes intestinal susceptibility to acute colitis.
The Journal of biological chemistry287(30)25631-9(2012 Jul)
Bel S,Elkis Y,Lerer-Goldstein T,Nyska A,Shpungin S,Nir U

CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion.
The EMBO journal31(10)2309-21(2012 May)
Gu P,Min JN,Wang Y,Huang C,Peng T,Chai W,Chang S